pycom05g30130

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0007930
Physical Location & Seq
Forward (+)
30350612 .. 30352031
1420 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g30130.1

Sequence Viewer

Length: 1329 bp
ATGGCGTCACTCCACCCCTCCGAGTTGGACTCCGCCGCCACCGACTCGGTCGCCTCGACCCCGCGCTCGGACCACCCGTCTCACGATCTCAACTCGCGTGTACGGTTCATGTGCAGCTTCGGCGGCAAGATCTTGCCTCGTCCGCACGACAATCAGCTTCGCTATGTCGGAGGCGATACCCGAATTGTCGCCGTCCAGCGCGCCACCTCTTTCTCCGCCCTTCTCTCGAAGCTTTCTAAACTCTCAGGGTTGAGTGACGTGACGGTGAAGTACCAGCTGCCGAACGAGGACCTCGACTCGTTGATCTCTGTCACCACCGACGAGGACGTCGACAACATGATGGAGGAGTACGACCGCATCGCACAGAATCAAAACTCAAAGTCGGCGCGGCTCCGCCTCTTTCTCTTCGTCAAAGGCAGCGACGATCCGATTAATTCGCGATCCTCCAGCATCAGCTCGCTCCTCGACGGCTCCGCCAAGCGTGAGCTCTGGTTCCTCGACGCCCTCAACAGCGGCGCGTCCAATTCCGCGATGCTCGAGCGGAACCGCTCCGAAGCTTCTTCGATCTTCTCCGAAGTGCCTGACTACTTATTCGGGTTGGACAATTCCGACGACACTCACTCTCGCGGCGGCGAGTTCAAACCGAAGGCCCGACCCGTATTGCACGACAACGTGTCGGTTTCGGATCCGGGTTCACCTGCCCCGGTAACTTCCTCGCCGTTCTGCTCGACGTCGTCTGTGCCATCTGTGCCGTCGATGCCCAGTCTACCACCGGTGAAAACCCGACCCGATTCCAATCAAGCCATCGAGACGAAAGAGAATCAATCGGAGGGATTTTCCGAGACGGTTGAGTTGCCCGTGTCGCAAGCAACTGGGTTCGTGGGCAACCCCGGGGTGCATTATATGTCGGATCCTAATTACCAGGGGCATATGGTTCAGCCAGTACCCGTTTACTACTATCCGGGTCAGGTTCCACCCACAACCGTACAGGTCCAGCCGTTTCCGATTCAGAGTCAGTATGTCCATCAGCAATACCACCAAGTAGGGAGTCAGATTCCAGTGGGGTACCATAACCCAATACCAGGTATGGGTCAAATATATGGTGGAGGGTTGAGGCCAGGGGCGGCAATGGACCCGCGTGACGTGTCAGCTCGGGTGGTTTCCAACGGAGTGAGCCAGCAGCAGCAGCAGGTGTTTTATGGAGTGAGGAATGCTGCGGTGGTTCCGCCGTATAATCCAGGTATGGTGGTGCAATCGGAGGAGGAATGGCAAGGACCCAGACCCGGACCCGAGATGAATAAGGGTCGGGCTCCGAATGCATCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

443

Amino Acids

47.96

Weight (kDa)

5.47

Isoelectric Point (pI)

54.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 49 - 137 1.8e-20 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 706, 1180
AasI GACNNNNNNGTC 1 cut(s) 326
AatII GACGTC 2 cut(s) 330, 734
Acc36I ACCTGC 2 cut(s) 706, 1180
Acc65I GGTACC 1 cut(s) 1065
AccB1I GGYRCC 1 cut(s) 1065
AccBSI CCGCTC 2 cut(s) 541, 549
AccI GTMKAC 2 cut(s) 330, 766
AccII CGCG 9 cut(s) 64, 97, 201, 388, 439, 518, 530, 627, 1138
AclWI GGATC 6 cut(s) 419, 435, 680, 693, 905, 918
AcyI GRCGYC 4 cut(s) 5, 327, 501, 731
AfaI GTAC 6 cut(s) 102, 272, 350, 945, 987, 1067
AfiI CCNNNNNNNGG 4 cut(s) 67, 1082, 1088, 1283
AflIII ACRYGT 2 cut(s) 672, 1143
AgeI ACCGGT 1 cut(s) 772
AgsI TTSAA 1 cut(s) 640
AhdI GACNNNNNGTC 2 cut(s) 76, 673
AjiI CACGTC 2 cut(s) 259, 1144
AjnI CCWGG 4 cut(s) 921, 1081, 1117, 1237
AluBI AGCT 8 cut(s) 117, 157, 232, 277, 456, 487, 557, 1151
AluI AGCT 8 cut(s) 117, 157, 232, 277, 456, 487, 557, 1151
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 3 cut(s) 84, 803, 836
AlwI GGATC 6 cut(s) 419, 435, 680, 693, 905, 918
Ama87I CYCGRG 4 cut(s) 536, 890, 1152, 1289
AoxI GGCC 2 cut(s) 648, 1115
ApeKI GCWGC 7 cut(s) 114, 277, 417, 1180, 1183, 1186, 1214
ArsI GACNNNNNNTTYG 2 cut(s) 575, 607
AseI ATTAAT 1 cut(s) 432
AsiGI ACCGGT 1 cut(s) 772
Asp718I GGTACC 1 cut(s) 1065
AspLEI GCGC 5 cut(s) 66, 201, 203, 388, 518
AspS9I GGNCC 7 cut(s) 70, 289, 649, 991, 1132, 1274, 1286
AsuC2I CCSGG 6 cut(s) 690, 704, 891, 892, 963, 1284
AsuHPI GGTGA 4 cut(s) 277, 304, 687, 787
AvaI CYCGRG 4 cut(s) 536, 890, 1152, 1289
AvaII GGWCC 6 cut(s) 70, 289, 991, 1132, 1274, 1286
BamHI GGATCC 2 cut(s) 685, 910
BanI GGYRCC 1 cut(s) 1065
BanII GRGCYC 2 cut(s) 489, 1312
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 7 cut(s) 126, 264, 429, 1192, 1195, 1198, 1201
BccI CCATC 4 cut(s) 334, 751, 812, 1032
BceAI ACGGC 6 cut(s) 176, 484, 703, 736, 982, 1213
BciT130I CCWGG 4 cut(s) 923, 1083, 1119, 1239
BcnI CCSGG 6 cut(s) 690, 704, 891, 892, 963, 1284
BcoDI GTCTC 3 cut(s) 84, 803, 836
BfuAI ACCTGC 2 cut(s) 706, 1180
BglII AGATCT 1 cut(s) 129
Bme18I GGWCC 6 cut(s) 70, 289, 991, 1132, 1274, 1286
BmeRI GACNNNNNGTC 2 cut(s) 76, 673
BmeT110I CYCGRG 4 cut(s) 536, 890, 1152, 1289
BmgBI CACGTC 2 cut(s) 259, 1144
BmgT120I GGNCC 7 cut(s) 70, 289, 649, 991, 1132, 1274, 1286
BmrI ACTGGG 2 cut(s) 756, 882
BmsI GCATC 4 cut(s) 366, 459, 522, 747
BmuI ACTGGG 2 cut(s) 756, 882
BplI GAGNNNNNCTC 2 cut(s) 14, 46
BpmI CTGGAG 1 cut(s) 430
BpuMI CCSGG 6 cut(s) 690, 704, 891, 892, 963, 1284
BsaBI GATNNNNATC 1 cut(s) 795
BsaHI GRCGYC 4 cut(s) 5, 327, 501, 731
BsaJI CCNNGG 6 cut(s) 702, 889, 890, 891, 922, 1118
BsaWI WCCGGW 1 cut(s) 772
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 1082, 1088, 1283
Bse118I RCCGGY 1 cut(s) 772
Bse1I ACTGG 4 cut(s) 762, 877, 941, 1058
Bse3DI GCAATG 1 cut(s) 1134
Bse8I GATNNNNATC 1 cut(s) 795
BseBI CCWGG 4 cut(s) 923, 1083, 1119, 1239
BseDI CCNNGG 6 cut(s) 702, 889, 890, 891, 922, 1118
BseJI GATNNNNATC 1 cut(s) 795
BseLI CCNNNNNNNGG 4 cut(s) 67, 1082, 1088, 1283
BseMI GCAATG 1 cut(s) 1134
BseMII CTCAG 1 cut(s) 258
BseNI ACTGG 4 cut(s) 762, 877, 941, 1058
BsePI GCGCGC 1 cut(s) 199
BseRI GAGGAG 3 cut(s) 359, 452, 1274
BseXI GCAGC 7 cut(s) 126, 264, 429, 1192, 1195, 1198, 1201
BsgI GTGCAG 1 cut(s) 133
Bsh1236I CGCG 9 cut(s) 64, 97, 201, 388, 439, 518, 530, 627, 1138
Bsh1285I CGRYCG 2 cut(s) 51, 355
BshFI GGCC 2 cut(s) 650, 1117
BshNI GGYRCC 1 cut(s) 1065
BshTI ACCGGT 1 cut(s) 772
BsiEI CGRYCG 2 cut(s) 51, 355
BsiHKAI GWGCWC 1 cut(s) 489
BsiHKCI CYCGRG 4 cut(s) 536, 890, 1152, 1289
BsiSI CCGG 6 cut(s) 689, 704, 773, 891, 962, 1284
BslI CCNNNNNNNGG 4 cut(s) 67, 1082, 1088, 1283
BsmAI GTCTC 3 cut(s) 84, 803, 836
BsmBI CGTCTC 3 cut(s) 84, 803, 836
BsmI GAATGC 2 cut(s) 1216, 1321
BsnI GGCC 2 cut(s) 650, 1117
BsoBI CYCGRG 4 cut(s) 536, 890, 1152, 1289
Bsp1286I GDGCHC 2 cut(s) 489, 1312
Bsp143I GATC 8 cut(s) 85, 129, 303, 424, 440, 564, 685, 910
Bsp68I TCGCGA 1 cut(s) 439
BspANI GGCC 2 cut(s) 650, 1117
BspCNI CTCAG 1 cut(s) 257
BspFNI CGCG 9 cut(s) 64, 97, 201, 388, 439, 518, 530, 627, 1138
BspMI ACCTGC 2 cut(s) 706, 1180
BspPI GGATC 6 cut(s) 419, 435, 680, 693, 905, 918
BspT107I GGYRCC 1 cut(s) 1065
BsrBI CCGCTC 2 cut(s) 541, 549
BsrDI GCAATG 1 cut(s) 1134
BsrFI RCCGGY 1 cut(s) 772
BsrI ACTGG 4 cut(s) 762, 877, 941, 1058
BssAI RCCGGY 1 cut(s) 772
BssECI CCNNGG 6 cut(s) 702, 889, 890, 891, 922, 1118
BssHII GCGCGC 1 cut(s) 199
BssMI GATC 8 cut(s) 85, 129, 303, 424, 440, 564, 685, 910
BssNI GRCGYC 4 cut(s) 5, 327, 501, 731
Bst2UI CCWGG 4 cut(s) 923, 1083, 1119, 1239
Bst4CI ACNGT 4 cut(s) 105, 265, 847, 985
Bst6I CTCTTC 1 cut(s) 410
BstACI GRCGYC 4 cut(s) 5, 327, 501, 731
BstC8I GCNNGC 4 cut(s) 201, 458, 867, 1178
BstDEI CTNAG 1 cut(s) 244
BstFNI CGCG 9 cut(s) 64, 97, 201, 388, 439, 518, 530, 627, 1138
BstHHI GCGC 5 cut(s) 66, 201, 203, 388, 518
BstKTI GATC 8 cut(s) 88, 132, 306, 427, 443, 567, 688, 913
BstMAI GTCTC 3 cut(s) 84, 803, 836
BstMBI GATC 8 cut(s) 85, 129, 303, 424, 440, 564, 685, 910
BstMCI CGRYCG 2 cut(s) 51, 355
BstMWI GCNNNNNNNGC 8 cut(s) 120, 123, 142, 748, 757, 862, 1186, 1316
BstNI CCWGG 4 cut(s) 923, 1083, 1119, 1239
BstUI CGCG 9 cut(s) 64, 97, 201, 388, 439, 518, 530, 627, 1138
BstV1I GCAGC 7 cut(s) 126, 264, 429, 1192, 1195, 1198, 1201
BstX2I RGATCY 3 cut(s) 129, 685, 910
BstYI RGATCY 3 cut(s) 129, 685, 910
BsuRI GGCC 2 cut(s) 650, 1117
BtgZI GCGATG 2 cut(s) 343, 545
BtrI CACGTC 2 cut(s) 259, 1144
BtsIMutI CAGTG 1 cut(s) 1065
BtuMI TCGCGA 1 cut(s) 439
BveI ACCTGC 2 cut(s) 706, 1180
Cac8I GCNNGC 4 cut(s) 201, 458, 867, 1178
CfoI GCGC 5 cut(s) 66, 201, 203, 388, 518
Cfr10I RCCGGY 1 cut(s) 772
Cfr13I GGNCC 7 cut(s) 70, 289, 649, 991, 1132, 1274, 1286
Cfr9I CCCGGG 1 cut(s) 890
CseI GACGC 2 cut(s) 507, 509
CsiI ACCWGGT 1 cut(s) 1081
Csp6I GTAC 6 cut(s) 101, 271, 349, 944, 986, 1066
CspAI ACCGGT 1 cut(s) 772
CviAII CATG 2 cut(s) 109, 337
CviQI GTAC 6 cut(s) 101, 271, 349, 944, 986, 1066
DdeI CTNAG 1 cut(s) 244
DpnI GATC 8 cut(s) 87, 131, 305, 426, 442, 566, 687, 912
DpnII GATC 8 cut(s) 85, 129, 303, 424, 440, 564, 685, 910
DrdI GACNNNNNNGTC 1 cut(s) 326
DriI GACNNNNNGTC 2 cut(s) 76, 673
DseDI GACNNNNNNGTC 1 cut(s) 326
Eam1104I CTCTTC 1 cut(s) 410
Eam1105I GACNNNNNGTC 2 cut(s) 76, 673
EarI CTCTTC 1 cut(s) 410
EciI GGCGGA 5 cut(s) 22, 205, 383, 463, 1215
Ecl136II GAGCTC 1 cut(s) 487
Eco24I GRGCYC 2 cut(s) 489, 1312
Eco47I GGWCC 6 cut(s) 70, 289, 991, 1132, 1274, 1286
Eco53kI GAGCTC 1 cut(s) 487
Eco88I CYCGRG 4 cut(s) 536, 890, 1152, 1289
EcoICRI GAGCTC 1 cut(s) 487
EcoO109I RGGNCCY 2 cut(s) 289, 1274
EcoRII CCWGG 4 cut(s) 921, 1081, 1117, 1237
EcoT22I ATGCAT 1 cut(s) 1321
EcoT38I GRGCYC 2 cut(s) 489, 1312
Esp3I CGTCTC 3 cut(s) 84, 803, 836
FaeI CATG 2 cut(s) 112, 340
FatI CATG 2 cut(s) 108, 336
FauI CCCGC 2 cut(s) 69, 1143
FauNDI CATATG 1 cut(s) 930
FblI GTMKAC 2 cut(s) 330, 766
FriOI GRGCYC 2 cut(s) 489, 1312
GlaI GCGC 5 cut(s) 65, 200, 202, 387, 517
GsuI CTGGAG 1 cut(s) 430
HaeIII GGCC 2 cut(s) 650, 1117
HapII CCGG 6 cut(s) 689, 704, 773, 891, 962, 1284
HgaI GACGC 2 cut(s) 507, 509
HhaI GCGC 5 cut(s) 66, 201, 203, 388, 518
Hin1I GRCGYC 4 cut(s) 5, 327, 501, 731
Hin1II CATG 2 cut(s) 112, 340
Hin6I GCGC 5 cut(s) 64, 199, 201, 386, 516
HinP1I GCGC 5 cut(s) 64, 199, 201, 386, 516
HincII GTYRAC 1 cut(s) 331
HindII GTYRAC 1 cut(s) 331
HindIII AAGCTT 2 cut(s) 230, 555
HpaII CCGG 6 cut(s) 689, 704, 773, 891, 962, 1284
HphI GGTGA 4 cut(s) 277, 304, 687, 787
Hpy166II GTNNAC 5 cut(s) 101, 331, 695, 767, 952
Hpy188III TCNNGA 5 cut(s) 83, 226, 438, 808, 1326
Hpy8I GTNNAC 5 cut(s) 101, 331, 695, 767, 952
Hpy99I CGWCG 9 cut(s) 323, 332, 425, 470, 503, 614, 733, 736, 757
HpyAV CCTTC 2 cut(s) 230, 640
HpyCH4III ACNGT 4 cut(s) 105, 265, 847, 985
HpyCH4IV ACGT 5 cut(s) 258, 327, 672, 731, 1143
HpyCH4V TGCA 5 cut(s) 114, 664, 898, 1252, 1319
HpyF10VI GCNNNNNNNGC 8 cut(s) 120, 123, 142, 748, 757, 862, 1186, 1316
HpyF3I CTNAG 1 cut(s) 244
HpySE526I ACGT 5 cut(s) 258, 327, 672, 731, 1143
Hsp92I GRCGYC 4 cut(s) 5, 327, 501, 731
Hsp92II CATG 2 cut(s) 112, 340
HspAI GCGC 5 cut(s) 64, 199, 201, 386, 516
KpnI GGTACC 1 cut(s) 1069
Kzo9I GATC 8 cut(s) 85, 129, 303, 424, 440, 564, 685, 910
LmnI GCTCC 5 cut(s) 396, 465, 476, 554, 1315
Lsp1109I GCAGC 7 cut(s) 126, 264, 429, 1192, 1195, 1198, 1201
LweI GCATC 4 cut(s) 366, 459, 522, 747
MabI ACCWGGT 1 cut(s) 1081
MaeII ACGT 5 cut(s) 258, 327, 672, 731, 1143
MaeIII GTNAC 6 cut(s) 6, 254, 259, 310, 706, 1139
MalI GATC 8 cut(s) 87, 131, 305, 426, 442, 566, 687, 912
MbiI CCGCTC 2 cut(s) 541, 549
MboI GATC 8 cut(s) 85, 129, 303, 424, 440, 564, 685, 910
MboII GAAGA 3 cut(s) 397, 552, 559
MflI RGATCY 3 cut(s) 129, 685, 910
MhlI GDGCHC 2 cut(s) 489, 1312
MluCI AATT 5 cut(s) 183, 433, 523, 604, 916
MlyI GAGTC 5 cut(s) 23, 38, 290, 1021, 1057
MmeI TCCRAC 6 cut(s) 6, 148, 579, 633, 888, 1188
Mph1103I ATGCAT 1 cut(s) 1321
MseI TTAA 1 cut(s) 432
MspA1I CMGCKG 2 cut(s) 277, 513
MspI CCGG 6 cut(s) 689, 704, 773, 891, 962, 1284
Mva1269I GAATGC 2 cut(s) 1216, 1321
MvaI CCWGG 4 cut(s) 923, 1083, 1119, 1239
MvnI CGCG 9 cut(s) 64, 97, 201, 388, 439, 518, 530, 627, 1138
MwoI GCNNNNNNNGC 8 cut(s) 120, 123, 142, 748, 757, 862, 1186, 1316
NciI CCSGG 6 cut(s) 690, 704, 891, 892, 963, 1284
NdeI CATATG 1 cut(s) 930
NdeII GATC 8 cut(s) 85, 129, 303, 424, 440, 564, 685, 910
NlaIII CATG 2 cut(s) 112, 340
NmuCI GTSAC 5 cut(s) 6, 254, 259, 310, 1139
NruI TCGCGA 1 cut(s) 439
NsiI ATGCAT 1 cut(s) 1321
PaeR7I CTCGAG 1 cut(s) 536
PaqCI CACCTGC 2 cut(s) 706, 1180
PauI GCGCGC 1 cut(s) 199
PcsI WCGNNNNNNNCGW 4 cut(s) 53, 74, 291, 327
PctI GAATGC 2 cut(s) 1216, 1321
PfeI GAWTC 5 cut(s) 367, 791, 820, 1006, 1054
PflFI GACNNNGTC 2 cut(s) 47, 733
PinAI ACCGGT 1 cut(s) 772
PleI GAGTC 5 cut(s) 23, 38, 290, 1020, 1056
PpsI GAGTC 5 cut(s) 23, 38, 290, 1020, 1056
PpuMI RGGWCCY 2 cut(s) 289, 1274
PshBI ATTAAT 1 cut(s) 432
Psp124BI GAGCTC 1 cut(s) 489
Psp5II RGGWCCY 2 cut(s) 289, 1274
Psp6I CCWGG 4 cut(s) 921, 1081, 1117, 1237
PspGI CCWGG 4 cut(s) 921, 1081, 1117, 1237
PspPI GGNCC 7 cut(s) 70, 289, 649, 991, 1132, 1274, 1286
PspPPI RGGWCCY 2 cut(s) 289, 1274
PspXI VCTCGAGB 1 cut(s) 536
PsuI RGATCY 3 cut(s) 129, 685, 910
PsyI GACNNNGTC 2 cut(s) 47, 733
PteI GCGCGC 1 cut(s) 199
PvuII CAGCTG 1 cut(s) 277
RruI TCGCGA 1 cut(s) 439
RsaI GTAC 6 cut(s) 102, 272, 350, 945, 987, 1067
RsaNI GTAC 6 cut(s) 101, 271, 349, 944, 986, 1066
SacI GAGCTC 1 cut(s) 489
SalI GTCGAC 1 cut(s) 329
SaqAI TTAA 1 cut(s) 432
Sau3AI GATC 8 cut(s) 85, 129, 303, 424, 440, 564, 685, 910
Sau96I GGNCC 7 cut(s) 70, 289, 649, 991, 1132, 1274, 1286
SchI GAGTC 5 cut(s) 23, 38, 290, 1021, 1057
SduI GDGCHC 2 cut(s) 489, 1312
SexAI ACCWGGT 1 cut(s) 1081
SfaNI GCATC 4 cut(s) 366, 459, 522, 747
Sfr274I CTCGAG 1 cut(s) 536
SgrAI CRCCGGYG 1 cut(s) 772
SinI GGWCC 6 cut(s) 70, 289, 991, 1132, 1274, 1286
SlaI CTCGAG 1 cut(s) 536
SmaI CCCGGG 1 cut(s) 892
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 5 cut(s) 183, 433, 523, 604, 916
SstI GAGCTC 1 cut(s) 489
TaaI ACNGT 4 cut(s) 105, 265, 847, 985
TaiI ACGT 5 cut(s) 261, 330, 675, 734, 1146
TaqII GACCGA 1 cut(s) 37
TasI AATT 5 cut(s) 183, 433, 523, 604, 916
TauI GCSGC 7 cut(s) 38, 126, 391, 516, 630, 633, 1127
TfiI GAWTC 5 cut(s) 367, 791, 820, 1006, 1054
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TscAI CASTG 1 cut(s) 1065
TseFI GTSAC 5 cut(s) 6, 254, 259, 310, 1139
TseI GCWGC 7 cut(s) 114, 277, 417, 1180, 1183, 1186, 1214
Tsp45I GTSAC 5 cut(s) 6, 254, 259, 310, 1139
TspDTI ATGAA 2 cut(s) 97, 1310
TspGWI ACGGA 1 cut(s) 1182
TspMI CCCGGG 1 cut(s) 890
TspRI CASTG 1 cut(s) 1065
Tth111I GACNNNGTC 2 cut(s) 47, 733
VpaK11BI GGWCC 6 cut(s) 70, 289, 991, 1132, 1274, 1286
VspI ATTAAT 1 cut(s) 432
XhoI CTCGAG 1 cut(s) 536
XmaI CCCGGG 1 cut(s) 890
XmiI GTMKAC 2 cut(s) 330, 766
ZraI GACGTC 2 cut(s) 328, 732
Zsp2I ATGCAT 1 cut(s) 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.