AT4G05530

Enoyl-(Acyl carrier protein) reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015374
Physical Location & Seq
Forward (+)
2816317 .. 2818518
2202 bp
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UTR
Exon/CDS
Intron
AT4G05530.1

Sequence Viewer

Length: 765 bp
ATGGAGAAGAAGCTACCGAGAAGATTGGAAGGCAAAGTCGCGATTGTGACGGCTTCGACGCAAGGGATTGGTTTCGGAATCACTGAGCGTTTCGGTCTCGAAGGCGCTTCCGTCGTCGTCTCTTCCCGCAAACAGGCAAATGTTGATGAGGCAGTAGCAAAACTCAAATCCAAAGGGATTGATGCATATGGAATCGTCTGTCATGTTTCCAATGCTCAACATCGCCGCAATCTTGTCGAAAAGACGGTTCAGAAATATGGGAAGATAGATATCGTTGTCTGTAACGCTGCTGCCAATCCATCTACAGACCCAATCTTGTCTAGCAAAGAAGCTGTTCTTGACAAGCTTTGGGAAATCAATGTCAAATCCTCTATACTTCTCCTGCAGGATATGGCTCCTCACCTTGAGAAGGGTTCTTCTGTTATCTTCATAACTTCTATTGCTGGATTTTCACCACAAGGAGCTATGGCTATGTATGGTGTCACTAAAACTGCTCTTCTCGGACTAACTAAGGCGCTTGCTGCTGAGATGGCCCCCGATACACGTGTGAACGCAGTTGCACCCGGTTTTGTGCCCACACACTTTGCTTCTTTCATCACCGGTAGCTCTGAAGTGAGGGAAGGTATTGAAGAGAAGACTCTGCTTAACAGGTTGGGAACAACGGGAGACATGGCTGCTGCGGCTGCTTTCTTAGCATCAGATGATTCTTCTTACATCACTGGAGAAACTTTGGTTGTCGCTGGAGGAATGCCTTCAAGACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000253 GO:0001101 GO:0001523 GO:0003674 GO:0003824 GO:0004033 GO:0004090 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005783 GO:0005789 GO:0005829 GO:0006066 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007275 GO:0008104 GO:0008106 GO:0008150 GO:0008152 GO:0008202 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009850 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010243 GO:0010817 GO:0012505 GO:0014070 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016049 GO:0016101 GO:0016491 GO:0016614 GO:0016616 GO:0016651 GO:0016655 GO:0018455 GO:0019752 GO:0021700 GO:0022607 GO:0022622 GO:0030154 GO:0031907 GO:0031974 GO:0031984 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0034308 GO:0034613 GO:0034641 GO:0034754 GO:0040007 GO:0042175 GO:0042180 GO:0042221 GO:0042430 GO:0042445 GO:0042572 GO:0042579 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050896 GO:0051179 GO:0051234 GO:0051259 GO:0051262 GO:0051641 GO:0051649 GO:0052650 GO:0055114 GO:0060560 GO:0065003 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080024 GO:0080026 GO:0080147 GO:0090558 GO:0090627 GO:0098827 GO:0099402 GO:1901360 GO:1901564 GO:1901615 GO:1901698 GO:1901700 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 ko04146 map00830 map01100 map04146
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

254

Amino Acids

26.76

Weight (kDa)

8.46

Isoelectric Point (pI)

33.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 12 - 196 5.7e-41 short chain dehydrogenase
SDR PF23441 13 - 213 1.8e-11 SDR-like rossmann domain
KR PF08659 15 - 179 2.2e-07 KR domain
adh_short_C2 PF13561 18 - 250 1.1e-58 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015374)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05530
fragaria_vesca FvH4_3g01160
malus_domestica MD05G1355400.v1.1 MD05G1355800.v1.1 MD10G1329100.v1.1
prunus_persica Prupe.4G012900_v2.0.a1
pyrus_communis pycom10g27890
rosa_chinensis RchiOBHm_Chr5g0001721
rosa_laevigata RLG00000030962
rosa_multiflora Rmu_ssc0000237.1_g000021
rosa_roxburghii Rroxscaffold_1G00071180 Rroxscaffold_1G00074590
rosa_rugosa Rorug04G0392200
rosa_samantha Rh5AG013600 Rh5CG014600
rosa_wichuraiana Rw5G001320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 41
AciI CCGC 3 cut(s) 127, 226, 680
AcuI CTGAAG 1 cut(s) 630
AcvI CACGTG 1 cut(s) 545
AfiI CCNNNNNNNGG 2 cut(s) 133, 409
AflIII ACRYGT 2 cut(s) 542, 544
AgeI ACCGGT 1 cut(s) 599
AgsI TTSAA 2 cut(s) 629, 756
AluBI AGCT 5 cut(s) 13, 332, 346, 464, 606
AluI AGCT 5 cut(s) 13, 332, 346, 464, 606
Alw26I GTCTC 3 cut(s) 101, 124, 660
AoxI GGCC 1 cut(s) 531
ApeKI GCWGC 6 cut(s) 287, 290, 521, 674, 677, 683
AsiGI ACCGGT 1 cut(s) 599
Asp700I GAANNNNTTC 2 cut(s) 333, 751
AspLEI GCGC 2 cut(s) 107, 517
AspS9I GGNCC 1 cut(s) 532
AsuC2I CCSGG 1 cut(s) 564
AsuHPI GGTGA 3 cut(s) 392, 444, 589
BaeGI GKGCMC 1 cut(s) 576
BaeI ACNNNNGTAYC 2 cut(s) 531, 564
BbrPI CACGTG 1 cut(s) 545
BbsI GAAGAC 1 cut(s) 641
BbvI GCAGC 6 cut(s) 274, 277, 508, 661, 664, 670
BccI CCATC 2 cut(s) 307, 523
BceAI ACGGC 1 cut(s) 66
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BcnI CCSGG 1 cut(s) 564
BcoDI GTCTC 3 cut(s) 101, 124, 660
BfaI CTAG 1 cut(s) 321
BfmI CTRYAG 2 cut(s) 303, 383
BfoI RGCGCY 2 cut(s) 108, 518
BisI GCNGC 8 cut(s) 226, 288, 291, 522, 675, 678, 681, 684
BlsI GCNGC 8 cut(s) 227, 289, 292, 523, 676, 679, 682, 685
Bme1390I CCNGG 1 cut(s) 564
BmgT120I GGNCC 1 cut(s) 532
BmiI GGNNCC 2 cut(s) 396, 534
BmrFI CCNGG 1 cut(s) 564
BmsI GCATC 2 cut(s) 172, 704
BpiI GAAGAC 1 cut(s) 641
BpmI CTGGAG 2 cut(s) 741, 762
BpuEI CTTGAG 1 cut(s) 425
BpuMI CCSGG 1 cut(s) 564
BsaAI YACGTR 1 cut(s) 545
BsaBI GATNNNNATC 1 cut(s) 269
BsaI GGTCTC 1 cut(s) 101
BsaWI WCCGGW 1 cut(s) 599
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 409
Bse118I RCCGGY 1 cut(s) 599
Bse1I ACTGG 1 cut(s) 724
Bse8I GATNNNNATC 1 cut(s) 269
BseJI GATNNNNATC 1 cut(s) 269
BseLI CCNNNNNNNGG 2 cut(s) 133, 409
BseMII CTCAG 2 cut(s) 75, 516
BseNI ACTGG 1 cut(s) 724
BseRI GAGGAG 1 cut(s) 387
BseSI GKGCMC 1 cut(s) 576
BseXI GCAGC 6 cut(s) 274, 277, 508, 661, 664, 670
Bsh1236I CGCG 1 cut(s) 41
BshFI GGCC 1 cut(s) 533
BshTI ACCGGT 1 cut(s) 599
BsiSI CCGG 2 cut(s) 564, 600
BslI CCNNNNNNNGG 2 cut(s) 133, 409
BsmAI GTCTC 3 cut(s) 101, 124, 660
BsmBI CGTCTC 1 cut(s) 124
BsmI GAATGC 1 cut(s) 753
BsnI GGCC 1 cut(s) 533
Bso31I GGTCTC 1 cut(s) 101
Bsp1286I GDGCHC 1 cut(s) 576
Bsp68I TCGCGA 1 cut(s) 41
BspACI CCGC 3 cut(s) 127, 226, 680
BspANI GGCC 1 cut(s) 533
BspCNI CTCAG 2 cut(s) 76, 517
BspFNI CGCG 1 cut(s) 41
BspLI GGNNCC 2 cut(s) 396, 534
BspMAI CTGCAG 1 cut(s) 387
BspQI GCTCTTC 1 cut(s) 501
BspTNI GGTCTC 1 cut(s) 101
BsrFI RCCGGY 1 cut(s) 599
BsrI ACTGG 1 cut(s) 724
BssAI RCCGGY 1 cut(s) 599
Bst4CI ACNGT 1 cut(s) 247
Bst6I CTCTTC 3 cut(s) 127, 501, 624
BstBAI YACGTR 1 cut(s) 545
BstC8I GCNNGC 1 cut(s) 519
BstDEI CTNAG 4 cut(s) 84, 510, 525, 691
BstENI CCTNNNNNAGG 1 cut(s) 407
BstFNI CGCG 1 cut(s) 41
BstH2I RGCGCY 2 cut(s) 108, 518
BstHHI GCGC 2 cut(s) 107, 517
BstMAI GTCTC 3 cut(s) 101, 124, 660
BstMWI GCNNNNNNNGC 5 cut(s) 521, 530, 680, 683, 692
BstSCI CCNGG 1 cut(s) 562
BstSFI CTRYAG 2 cut(s) 303, 383
BstSLI GKGCMC 1 cut(s) 576
BstUI CGCG 1 cut(s) 41
BstV1I GCAGC 6 cut(s) 274, 277, 508, 661, 664, 670
BstV2I GAAGAC 1 cut(s) 641
BsuRI GGCC 1 cut(s) 533
BtgZI GCGATG 1 cut(s) 206
BtsIMutI CAGTG 2 cut(s) 81, 717
BtuMI TCGCGA 1 cut(s) 41
Cac8I GCNNGC 1 cut(s) 519
CfoI GCGC 2 cut(s) 107, 517
Cfr10I RCCGGY 1 cut(s) 599
Cfr13I GGNCC 1 cut(s) 532
CseI GACGC 1 cut(s) 67
CspAI ACCGGT 1 cut(s) 599
CspCI CAANNNNNGTGG 2 cut(s) 565, 600
CviAII CATG 2 cut(s) 203, 670
DdeI CTNAG 4 cut(s) 84, 510, 525, 691
Eam1104I CTCTTC 3 cut(s) 127, 501, 624
EarI CTCTTC 3 cut(s) 127, 501, 624
Eco31I GGTCTC 1 cut(s) 101
Eco32I GATATC 1 cut(s) 271
Eco57I CTGAAG 1 cut(s) 630
Eco72I CACGTG 1 cut(s) 545
EcoNI CCTNNNNNAGG 1 cut(s) 407
EcoRV GATATC 1 cut(s) 271
EcoT22I ATGCAT 1 cut(s) 187
Esp3I CGTCTC 1 cut(s) 124
FaeI CATG 2 cut(s) 206, 673
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FatI CATG 2 cut(s) 202, 669
FauI CCCGC 1 cut(s) 134
FauNDI CATATG 1 cut(s) 187
Fnu4HI GCNGC 8 cut(s) 226, 288, 291, 522, 675, 678, 681, 684
Fsp4HI GCNGC 8 cut(s) 226, 288, 291, 522, 675, 678, 681, 684
FspBI CTAG 1 cut(s) 321
GlaI GCGC 2 cut(s) 106, 516
GluI GCNGC 8 cut(s) 226, 288, 291, 522, 675, 678, 681, 684
GsuI CTGGAG 2 cut(s) 741, 762
HaeII RGCGCY 2 cut(s) 108, 518
HaeIII GGCC 1 cut(s) 533
HapII CCGG 2 cut(s) 564, 600
HgaI GACGC 1 cut(s) 67
HhaI GCGC 2 cut(s) 107, 517
Hin1II CATG 2 cut(s) 206, 673
Hin6I GCGC 2 cut(s) 105, 515
HinP1I GCGC 2 cut(s) 105, 515
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 5 cut(s) 78, 192, 637, 704, 759
HpaII CCGG 2 cut(s) 564, 600
HphI GGTGA 3 cut(s) 392, 444, 589
Hpy166II GTNNAC 1 cut(s) 550
Hpy188I TCNGA 5 cut(s) 77, 252, 503, 610, 700
Hpy188III TCNNGA 4 cut(s) 40, 98, 338, 756
Hpy8I GTNNAC 1 cut(s) 550
Hpy99I CGWCG 3 cut(s) 61, 116, 119
HpyAV CCTTC 5 cut(s) 23, 95, 403, 614, 762
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 544
HpyCH4V TGCA 3 cut(s) 185, 385, 560
HpyF10VI GCNNNNNNNGC 5 cut(s) 521, 530, 680, 683, 692
HpyF3I CTNAG 4 cut(s) 84, 510, 525, 691
HpySE526I ACGT 1 cut(s) 544
Hsp92II CATG 2 cut(s) 206, 673
HspAI GCGC 2 cut(s) 105, 515
LguI GCTCTTC 1 cut(s) 501
LmnI GCTCC 2 cut(s) 400, 461
LpnPI CCDG 9 cut(s) 119, 371, 395, 429, 577, 613, 634, 705, 726
Lsp1109I GCAGC 6 cut(s) 274, 277, 508, 661, 664, 670
LweI GCATC 2 cut(s) 172, 704
MaeI CTAG 1 cut(s) 321
MaeII ACGT 1 cut(s) 544
MaeIII GTNAC 3 cut(s) 46, 281, 481
MhlI GDGCHC 1 cut(s) 576
MlyI GAGTC 2 cut(s) 631, 753
MnlI CCTC 5 cut(s) 142, 379, 408, 609, 737
Mph1103I ATGCAT 1 cut(s) 187
MroXI GAANNNNTTC 2 cut(s) 333, 751
MseI TTAA 1 cut(s) 645
MspI CCGG 2 cut(s) 564, 600
MspR9I CCNGG 1 cut(s) 564
Mva1269I GAATGC 1 cut(s) 753
MvnI CGCG 1 cut(s) 41
MwoI GCNNNNNNNGC 5 cut(s) 521, 530, 680, 683, 692
NciI CCSGG 1 cut(s) 564
NdeI CATATG 1 cut(s) 187
NlaIII CATG 2 cut(s) 206, 673
NlaIV GGNNCC 2 cut(s) 396, 534
NmuCI GTSAC 2 cut(s) 46, 481
NruI TCGCGA 1 cut(s) 41
NsiI ATGCAT 1 cut(s) 187
PciSI GCTCTTC 1 cut(s) 501
PctI GAATGC 1 cut(s) 753
PdmI GAANNNNTTC 2 cut(s) 333, 751
PfeI GAWTC 3 cut(s) 78, 192, 704
PinAI ACCGGT 1 cut(s) 599
PkrI GCNGC 8 cut(s) 227, 289, 292, 523, 676, 679, 682, 685
PleI GAGTC 2 cut(s) 631, 753
PmaCI CACGTG 1 cut(s) 545
PmlI CACGTG 1 cut(s) 545
PpsI GAGTC 2 cut(s) 631, 753
Ppu21I YACGTR 1 cut(s) 545
PspCI CACGTG 1 cut(s) 545
PspN4I GGNNCC 2 cut(s) 396, 534
PspPI GGNCC 1 cut(s) 532
PstI CTGCAG 1 cut(s) 387
RruI TCGCGA 1 cut(s) 41
SapI GCTCTTC 1 cut(s) 501
SaqAI TTAA 1 cut(s) 645
SatI GCNGC 8 cut(s) 226, 288, 291, 522, 675, 678, 681, 684
Sau96I GGNCC 1 cut(s) 532
SbfI CCTGCAGG 1 cut(s) 387
SchI GAGTC 2 cut(s) 631, 753
ScrFI CCNGG 1 cut(s) 564
SdaI CCTGCAGG 1 cut(s) 387
SduI GDGCHC 1 cut(s) 576
SetI ASST 9 cut(s) 15, 334, 348, 405, 466, 547, 608, 625, 653
SfaNI GCATC 2 cut(s) 172, 704
SfcI CTRYAG 2 cut(s) 303, 383
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
Sse8387I CCTGCAGG 1 cut(s) 387
SsiI CCGC 3 cut(s) 127, 226, 680
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 1 cut(s) 562
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 547
TaqI TCGA 3 cut(s) 56, 99, 237
TaqII GACCGA 1 cut(s) 83
TauI GCSGC 2 cut(s) 228, 683
TfiI GAWTC 3 cut(s) 78, 192, 704
Tru1I TTAA 1 cut(s) 645
Tru9I TTAA 1 cut(s) 645
TscAI CASTG 2 cut(s) 88, 724
TseFI GTSAC 2 cut(s) 46, 481
TseI GCWGC 6 cut(s) 287, 290, 521, 674, 677, 683
Tsp45I GTSAC 2 cut(s) 46, 481
TspDTI ATGAA 2 cut(s) 418, 583
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 2 cut(s) 88, 724
XagI CCTNNNNNAGG 1 cut(s) 407
XmnI GAANNNNTTC 2 cut(s) 333, 751
XspI CTAG 1 cut(s) 321
Zsp2I ATGCAT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.