MD05G1355800.v1.1

Dehydrogenase reductase SDR family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015374
Physical Location & Seq
Forward (+)
47193832 .. 47195989
2158 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1355800.v1.1.491

Sequence Viewer

Length: 771 bp
ATGGAGAAATCGAAGATTGGGAGGAGGTTCCAAGGCAAGGTAGCGATTGTGACGGCTTCCACTCAGGGCATCGGCTTCGGCATCGCCGAGCGCCTGGGGTTGGAAGGCGCCTCCGTCGTCATCTCCTCCCGCAAGCAGAAAAACGTTGATGAAGCAGTTGAGAAACTTAAAGCTGAAGGAATTGAGGCGTTGGGAGTAGTTTGCCACGTTTCGAATGAGCAACAAAGGAAGAATCTCATCAACAAGACTGTGCAGAAATACGGAAAAATAGATGTGGTTGTATCAAATGCTGCTGCCAATCCATCAGTTGACCCCATATTGCAAACCCAAGAATCTGTGCTTGACAAGCTATGGGAAATCAATGTCAAAGCTACTATACTACTTCTCAAGGATGCATCTCCGCACTTGACGAAGGGTTCTTCCGTTGTTATCATTTCCTCAATTGCTGGCTACAATCCACCAGGAGCTGCCATGGCTATGTATGGGGTCACTAAAACAGCCCTTCTTGGGTTAACCAAGGCCCTTGCCGGTGACATGGCGCCAGATACTCGTGTAAATTGTGTTGCTCCGGGTTTTGTTCCTACAAACTTTGCTTCATACATTACAGAAACTGATACCGTGAGGAAGGCCCTCGAGGAGAAGACGTTACTCAACAGGCTCGGTACCACTGGAGACATGGCTGCTGCCGCTGCCTTTTTGGCATCCGATGATGCTTCTTACATAACCGGAGAAACTATAGTAGTGGCCGGAGGGATGCCCTCTAGACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000253 GO:0001101 GO:0001523 GO:0003674 GO:0003824 GO:0004033 GO:0004090 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005783 GO:0005789 GO:0005829 GO:0006066 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007275 GO:0008104 GO:0008106 GO:0008150 GO:0008152 GO:0008202 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009850 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010243 GO:0010817 GO:0012505 GO:0014070 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016049 GO:0016101 GO:0016491 GO:0016614 GO:0016616 GO:0016651 GO:0016655 GO:0018455 GO:0019752 GO:0021700 GO:0022607 GO:0022622 GO:0030154 GO:0031907 GO:0031974 GO:0031984 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0034308 GO:0034613 GO:0034641 GO:0034754 GO:0040007 GO:0042175 GO:0042180 GO:0042221 GO:0042430 GO:0042445 GO:0042572 GO:0042579 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050896 GO:0051179 GO:0051234 GO:0051259 GO:0051262 GO:0051641 GO:0051649 GO:0052650 GO:0055114 GO:0060560 GO:0065003 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080024 GO:0080026 GO:0080147 GO:0090558 GO:0090627 GO:0098827 GO:0099402 GO:1901360 GO:1901564 GO:1901615 GO:1901698 GO:1901700 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 ko04146 map00830 map01100 map04146
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

257

Amino Acids

26.93

Weight (kDa)

8.75

Isoelectric Point (pI)

27.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 204 5.8e-42 short chain dehydrogenase
SDR PF23441 15 - 251 8.3e-15 SDR-like rossmann domain
KR PF08659 16 - 151 4.9e-09 KR domain
adh_short_C2 PF13561 19 - 252 1.3e-59 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015374)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05530
fragaria_vesca FvH4_3g01160
malus_domestica MD05G1355400.v1.1 MD05G1355800.v1.1 MD10G1329100.v1.1
prunus_persica Prupe.4G012900_v2.0.a1
pyrus_communis pycom10g27890
rosa_chinensis RchiOBHm_Chr5g0001721
rosa_laevigata RLG00000030962
rosa_multiflora Rmu_ssc0000237.1_g000021
rosa_roxburghii Rroxscaffold_1G00071180 Rroxscaffold_1G00074590
rosa_rugosa Rorug04G0392200
rosa_samantha Rh5AG013600 Rh5CG014600
rosa_wichuraiana Rw5G001320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 632
Acc65I GGTACC 1 cut(s) 662
AccB1I GGYRCC 3 cut(s) 107, 538, 662
AciI CCGC 3 cut(s) 130, 401, 687
AclI AACGTT 1 cut(s) 144
AcoI YGGCCR 1 cut(s) 744
AcuI CTGAAG 1 cut(s) 195
AcyI GRCGYC 2 cut(s) 108, 539
AfaI GTAC 1 cut(s) 664
AfiI CCNNNNNNNGG 3 cut(s) 37, 100, 507
AjnI CCWGG 2 cut(s) 93, 460
AluBI AGCT 4 cut(s) 173, 349, 371, 467
AluI AGCT 4 cut(s) 173, 349, 371, 467
Alw26I GTCTC 1 cut(s) 666
AlwNI CAGNNNCTG 2 cut(s) 467, 611
Ama87I CYCGRG 1 cut(s) 632
AoxI GGCC 3 cut(s) 519, 627, 744
ApeKI GCWGC 6 cut(s) 290, 293, 467, 680, 683, 689
Asp718I GGTACC 1 cut(s) 662
AspLEI GCGC 3 cut(s) 93, 110, 541
AspS9I GGNCC 2 cut(s) 520, 628
AsuC2I CCSGG 1 cut(s) 570
AsuHPI GGTGA 1 cut(s) 542
AsuII TTCGAA 1 cut(s) 212
AvaI CYCGRG 1 cut(s) 632
BaeI ACNNNNGTAYC 2 cut(s) 537, 570
BanI GGYRCC 3 cut(s) 107, 538, 662
BauI CACGAG 1 cut(s) 549
BbsI GAAGAC 1 cut(s) 647
BbvI GCAGC 6 cut(s) 277, 280, 454, 667, 670, 676
BccI CCATC 1 cut(s) 310
BceAI ACGGC 1 cut(s) 69
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 2 cut(s) 95, 462
BcnI CCSGG 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 666
BfaI CTAG 2 cut(s) 762, 769
BfmI CTRYAG 1 cut(s) 735
BfoI RGCGCY 3 cut(s) 94, 111, 542
BglI GCCNNNNNGGC 1 cut(s) 698
BisI GCNGC 7 cut(s) 291, 294, 468, 681, 684, 687, 690
BlsI GCNGC 7 cut(s) 292, 295, 469, 682, 685, 688, 691
Bme1390I CCNGG 3 cut(s) 95, 462, 570
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 2 cut(s) 520, 628
BmiI GGNNCC 4 cut(s) 29, 109, 540, 664
BmrFI CCNGG 3 cut(s) 95, 462, 570
BmsI GCATC 7 cut(s) 78, 90, 382, 404, 700, 710, 744
BpiI GAAGAC 1 cut(s) 647
BpmI CTGGAG 1 cut(s) 690
Bpu14I TTCGAA 1 cut(s) 212
BpuEI CTTGAG 1 cut(s) 371
BpuMI CCSGG 1 cut(s) 570
BsaHI GRCGYC 2 cut(s) 108, 539
BsaJI CCNNGG 4 cut(s) 31, 94, 471, 516
BsaWI WCCGGW 1 cut(s) 725
BsaXI ACNNNNNCTCC 2 cut(s) 629, 659
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 100, 507
Bse118I RCCGGY 1 cut(s) 527
Bse1I ACTGG 1 cut(s) 673
BseBI CCWGG 2 cut(s) 95, 462
BseDI CCNNGG 4 cut(s) 31, 94, 471, 516
BseGI GGATG 3 cut(s) 397, 701, 759
BseLI CCNNNNNNNGG 3 cut(s) 37, 100, 507
BseMII CTCAG 1 cut(s) 77
BseNI ACTGG 1 cut(s) 673
BseRI GAGGAG 3 cut(s) 37, 115, 650
BseXI GCAGC 6 cut(s) 277, 280, 454, 667, 670, 676
BsgI GTGCAG 1 cut(s) 272
BshFI GGCC 3 cut(s) 521, 629, 746
BshNI GGYRCC 3 cut(s) 107, 538, 662
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 4 cut(s) 528, 569, 726, 747
BslI CCNNNNNNNGG 3 cut(s) 37, 100, 507
BsmAI GTCTC 1 cut(s) 666
BsnI GGCC 3 cut(s) 521, 629, 746
BsoBI CYCGRG 1 cut(s) 632
Bsp119I TTCGAA 1 cut(s) 212
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 3 cut(s) 130, 401, 687
BspANI GGCC 3 cut(s) 521, 629, 746
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 4 cut(s) 29, 109, 540, 664
BspT104I TTCGAA 1 cut(s) 212
BspT107I GGYRCC 3 cut(s) 107, 538, 662
BsrFI RCCGGY 1 cut(s) 527
BsrI ACTGG 1 cut(s) 673
BssAI RCCGGY 1 cut(s) 527
BssECI CCNNGG 4 cut(s) 31, 94, 471, 516
BssNI GRCGYC 2 cut(s) 108, 539
BssSI CACGAG 1 cut(s) 549
BssT1I CCWWGG 3 cut(s) 31, 471, 516
Bst2BI CACGAG 1 cut(s) 549
Bst2UI CCWGG 2 cut(s) 95, 462
Bst4CI ACNGT 2 cut(s) 250, 619
BstACI GRCGYC 2 cut(s) 108, 539
BstBI TTCGAA 1 cut(s) 212
BstC8I GCNNGC 2 cut(s) 134, 448
BstDEI CTNAG 1 cut(s) 63
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 3 cut(s) 397, 701, 759
BstH2I RGCGCY 3 cut(s) 94, 111, 542
BstHHI GCGC 3 cut(s) 93, 110, 541
BstMAI GTCTC 1 cut(s) 666
BstMWI GCNNNNNNNGC 5 cut(s) 346, 473, 686, 689, 698
BstNI CCWGG 2 cut(s) 95, 462
BstSCI CCNGG 3 cut(s) 93, 460, 568
BstSFI CTRYAG 1 cut(s) 735
BstV1I GCAGC 6 cut(s) 277, 280, 454, 667, 670, 676
BstV2I GAAGAC 1 cut(s) 647
BsuRI GGCC 3 cut(s) 521, 629, 746
BtgI CCRYGG 1 cut(s) 471
BtgZI GCGATG 1 cut(s) 67
BtsCI GGATG 3 cut(s) 397, 701, 759
BtsIMutI CAGTG 1 cut(s) 666
Cac8I GCNNGC 2 cut(s) 134, 448
CaiI CAGNNNCTG 2 cut(s) 467, 611
CfoI GCGC 3 cut(s) 93, 110, 541
Cfr10I RCCGGY 1 cut(s) 527
Cfr13I GGNCC 2 cut(s) 520, 628
Csp6I GTAC 1 cut(s) 663
CviAII CATG 3 cut(s) 472, 535, 676
CviQI GTAC 1 cut(s) 663
DdeI CTNAG 1 cut(s) 63
DinI GGCGCC 2 cut(s) 109, 540
EaeI YGGCCR 1 cut(s) 744
Eco130I CCWWGG 3 cut(s) 31, 471, 516
Eco57I CTGAAG 1 cut(s) 195
Eco88I CYCGRG 1 cut(s) 632
EcoO109I RGGNCCY 2 cut(s) 520, 628
EcoRII CCWGG 2 cut(s) 93, 460
EcoT14I CCWWGG 3 cut(s) 31, 471, 516
EcoT22I ATGCAT 1 cut(s) 397
EgeI GGCGCC 2 cut(s) 109, 540
EheI GGCGCC 2 cut(s) 109, 540
ErhI CCWWGG 3 cut(s) 31, 471, 516
FaeI CATG 3 cut(s) 475, 538, 679
FatI CATG 3 cut(s) 471, 534, 675
FauI CCCGC 1 cut(s) 137
Fnu4HI GCNGC 7 cut(s) 291, 294, 468, 681, 684, 687, 690
FokI GGATG 3 cut(s) 404, 688, 766
Fsp4HI GCNGC 7 cut(s) 291, 294, 468, 681, 684, 687, 690
FspBI CTAG 2 cut(s) 762, 769
GlaI GCGC 3 cut(s) 92, 109, 540
GluI GCNGC 7 cut(s) 291, 294, 468, 681, 684, 687, 690
GsuI CTGGAG 1 cut(s) 690
HaeII RGCGCY 3 cut(s) 94, 111, 542
HaeIII GGCC 3 cut(s) 521, 629, 746
HapII CCGG 4 cut(s) 528, 569, 726, 747
HhaI GCGC 3 cut(s) 93, 110, 541
Hin1I GRCGYC 2 cut(s) 108, 539
Hin1II CATG 3 cut(s) 475, 538, 679
Hin6I GCGC 3 cut(s) 91, 108, 539
HinP1I GCGC 3 cut(s) 91, 108, 539
HincII GTYRAC 2 cut(s) 310, 513
HindII GTYRAC 2 cut(s) 310, 513
HinfI GANTC 3 cut(s) 232, 332, 765
HpaI GTTAAC 1 cut(s) 513
HpaII CCGG 4 cut(s) 528, 569, 726, 747
HphI GGTGA 1 cut(s) 542
Hpy166II GTNNAC 2 cut(s) 310, 513
Hpy188I TCNGA 1 cut(s) 706
Hpy188III TCNNGA 1 cut(s) 762
Hpy8I GTNNAC 2 cut(s) 310, 513
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 5 cut(s) 98, 170, 406, 512, 619
HpyCH4III ACNGT 2 cut(s) 250, 619
HpyCH4IV ACGT 3 cut(s) 144, 207, 644
HpyCH4V TGCA 3 cut(s) 253, 322, 395
HpyF10VI GCNNNNNNNGC 5 cut(s) 346, 473, 686, 689, 698
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 3 cut(s) 144, 207, 644
Hsp92I GRCGYC 2 cut(s) 108, 539
Hsp92II CATG 3 cut(s) 475, 538, 679
HspAI GCGC 3 cut(s) 91, 108, 539
KasI GGCGCC 2 cut(s) 107, 538
KpnI GGTACC 1 cut(s) 666
KspAI GTTAAC 1 cut(s) 513
LmnI GCTCC 2 cut(s) 464, 571
Lsp1109I GCAGC 6 cut(s) 277, 280, 454, 667, 670, 676
LweI GCATC 7 cut(s) 78, 90, 382, 404, 700, 710, 744
MaeI CTAG 2 cut(s) 762, 769
MaeII ACGT 3 cut(s) 144, 207, 644
MaeIII GTNAC 4 cut(s) 49, 487, 530, 645
MboII GAAGA 4 cut(s) 25, 241, 411, 652
MfeI CAATTG 1 cut(s) 441
MluCI AATT 3 cut(s) 180, 441, 556
Mly113I GGCGCC 2 cut(s) 108, 539
MlyI GAGTC 1 cut(s) 759
MmeI TCCRAC 1 cut(s) 81
Mph1103I ATGCAT 1 cut(s) 397
MseI TTAA 2 cut(s) 168, 512
MslI CAYNNNNRTG 1 cut(s) 476
MspA1I CMGCKG 1 cut(s) 689
MspI CCGG 4 cut(s) 528, 569, 726, 747
MspR9I CCNGG 3 cut(s) 95, 462, 570
MunI CAATTG 1 cut(s) 441
MvaI CCWGG 2 cut(s) 95, 462
MwoI GCNNNNNNNGC 5 cut(s) 346, 473, 686, 689, 698
NarI GGCGCC 2 cut(s) 108, 539
NciI CCSGG 1 cut(s) 570
NcoI CCATGG 1 cut(s) 471
NlaIII CATG 3 cut(s) 475, 538, 679
NlaIV GGNNCC 4 cut(s) 29, 109, 540, 664
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 3 cut(s) 49, 487, 530
NsiI ATGCAT 1 cut(s) 397
NspV TTCGAA 1 cut(s) 212
PaeR7I CTCGAG 1 cut(s) 632
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PfeI GAWTC 2 cut(s) 232, 332
PkrI GCNGC 7 cut(s) 292, 295, 469, 682, 685, 688, 691
PleI GAGTC 1 cut(s) 759
PluTI GGCGCC 2 cut(s) 111, 542
PpsI GAGTC 1 cut(s) 759
Psp1406I AACGTT 1 cut(s) 144
Psp6I CCWGG 2 cut(s) 93, 460
PspGI CCWGG 2 cut(s) 93, 460
PspN4I GGNNCC 4 cut(s) 29, 109, 540, 664
PspPI GGNCC 2 cut(s) 520, 628
PspXI VCTCGAGB 1 cut(s) 632
PstNI CAGNNNCTG 2 cut(s) 467, 611
RsaI GTAC 1 cut(s) 664
RsaNI GTAC 1 cut(s) 663
RseI CAYNNNNRTG 1 cut(s) 476
SaqAI TTAA 2 cut(s) 168, 512
SatI GCNGC 7 cut(s) 291, 294, 468, 681, 684, 687, 690
Sau96I GGNCC 2 cut(s) 520, 628
SchI GAGTC 1 cut(s) 759
ScrFI CCNGG 3 cut(s) 95, 462, 570
SetI ASST 9 cut(s) 29, 42, 147, 175, 210, 351, 373, 469, 647
SfaNI GCATC 7 cut(s) 78, 90, 382, 404, 700, 710, 744
SfcI CTRYAG 1 cut(s) 735
SfoI GGCGCC 2 cut(s) 109, 540
Sfr274I CTCGAG 1 cut(s) 632
SfuI TTCGAA 1 cut(s) 212
SlaI CTCGAG 1 cut(s) 632
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 2 cut(s) 386, 632
SmoI CTYRAG 2 cut(s) 386, 632
Sse9I AATT 3 cut(s) 180, 441, 556
SsiI CCGC 3 cut(s) 130, 401, 687
SspDI GGCGCC 2 cut(s) 107, 538
SspMI CTAG 2 cut(s) 762, 769
StyD4I CCNGG 3 cut(s) 93, 460, 568
StyI CCWWGG 3 cut(s) 31, 471, 516
TaaI ACNGT 2 cut(s) 250, 619
TaiI ACGT 3 cut(s) 147, 210, 647
TaqI TCGA 3 cut(s) 11, 212, 633
TasI AATT 3 cut(s) 180, 441, 556
TauI GCSGC 1 cut(s) 689
TfiI GAWTC 2 cut(s) 232, 332
Tru1I TTAA 2 cut(s) 168, 512
Tru9I TTAA 2 cut(s) 168, 512
TscAI CASTG 1 cut(s) 673
TseFI GTSAC 3 cut(s) 49, 487, 530
TseI GCWGC 6 cut(s) 290, 293, 467, 680, 683, 689
Tsp45I GTSAC 3 cut(s) 49, 487, 530
TspDTI ATGAA 2 cut(s) 165, 585
TspGWI ACGGA 3 cut(s) 103, 276, 412
TspRI CASTG 1 cut(s) 673
XbaI TCTAGA 1 cut(s) 761
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XhoI CTCGAG 1 cut(s) 632
XspI CTAG 2 cut(s) 762, 769
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.