MD05G1355400.v1.1

Dehydrogenase reductase SDR family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0015374
Physical Location & Seq
Forward (+)
47169780 .. 47173519
3740 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1355400.v1.1.491

Sequence Viewer

Length: 771 bp
ATGGAGAAATCGAAGATTGGGATGAGGTTCCAAGGCAAGGTGGCGATTGTGACGACTTCCACTCAGGGCATCGGCTTCGGTATCGCCGAGTGCCTAGGTTTGGAAGGCGCCTCCGTCGTCATCTCCTCCCGCAAGCAGAAAAACGTTGATGAAGCAGTTGAGAAACTTAAAGCTCAAGGAATTGAGGCGTTGGGAGTCGTTTGCCACGTTTCGAATGAGCAACAAAGGAAGAATCTCATCAACAAGACTGTGCAGAAATACGGAAAAATAGATGTGGTTGTATCAAATGCTGCTGCCAATCCATCAGTTGACCCCATATTGCAAACCCAAGAATCTGTGCTTGACAAGCTATGGGAAATCAATGTCAAAGCTACTATACTACTTCTCAAGGATGCATCTCCGCACTTGACGAAGGGTTCTTCTGTTGTTATCATTTCCTCAATTGCTGGCTACAATCCACCGGGAGCTGCCATGGCTATGTACGGGGTCACTAAAACAGTCCTTCTTGGGCTAACCAAGGCTCTTGCCGGGGACATGGCGCCAGATACTCTTGTAAATTGTGTTGCTCCGGGTTTTGTTCCTACAAACTTTGCTTCATACATTACAGAAACCGATGCCGTGAGGAAGGCCCTCGAGGAGAAGACGTTACTCAACAGGCTCGGTACCACTGGAGACATGGCTGCTGCCGCTGCCTTTTTGGCATCCGATGATGCTTCTTACATAACCGGAGAAACCATAGTTGTGGCCGGAGGGATGCCCTCTAGACTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000253 GO:0001101 GO:0001523 GO:0003674 GO:0003824 GO:0004033 GO:0004090 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005777 GO:0005782 GO:0005783 GO:0005789 GO:0005829 GO:0006066 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006720 GO:0006721 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007275 GO:0008104 GO:0008106 GO:0008150 GO:0008152 GO:0008202 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009850 GO:0009888 GO:0009987 GO:0010015 GO:0010033 GO:0010053 GO:0010054 GO:0010243 GO:0010817 GO:0012505 GO:0014070 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016049 GO:0016101 GO:0016491 GO:0016614 GO:0016616 GO:0016651 GO:0016655 GO:0018455 GO:0019752 GO:0021700 GO:0022607 GO:0022622 GO:0030154 GO:0031907 GO:0031974 GO:0031984 GO:0032501 GO:0032502 GO:0033036 GO:0033365 GO:0034308 GO:0034613 GO:0034641 GO:0034754 GO:0040007 GO:0042175 GO:0042180 GO:0042221 GO:0042430 GO:0042445 GO:0042572 GO:0042579 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044255 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048364 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050896 GO:0051179 GO:0051234 GO:0051259 GO:0051262 GO:0051641 GO:0051649 GO:0052650 GO:0055114 GO:0060560 GO:0065003 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071695 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594 GO:0072662 GO:0072663 GO:0080024 GO:0080026 GO:0080147 GO:0090558 GO:0090627 GO:0098827 GO:0099402 GO:1901360 GO:1901564 GO:1901615 GO:1901698 GO:1901700 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 ko04146 map00830 map01100 map04146
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

257

Amino Acids

26.84

Weight (kDa)

7.65

Isoelectric Point (pI)

27.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 204 1.7e-38 short chain dehydrogenase
SDR PF23441 16 - 251 1.1e-13 SDR-like rossmann domain
KR PF08659 16 - 168 2.2e-09 KR domain
adh_short_C2 PF13561 21 - 252 3.8e-56 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015374)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G05530
fragaria_vesca FvH4_3g01160
malus_domestica MD05G1355400.v1.1 MD05G1355800.v1.1 MD10G1329100.v1.1
prunus_persica Prupe.4G012900_v2.0.a1
pyrus_communis pycom10g27890
rosa_chinensis RchiOBHm_Chr5g0001721
rosa_laevigata RLG00000030962
rosa_multiflora Rmu_ssc0000237.1_g000021
rosa_roxburghii Rroxscaffold_1G00071180 Rroxscaffold_1G00074590
rosa_rugosa Rorug04G0392200
rosa_samantha Rh5AG013600 Rh5CG014600
rosa_wichuraiana Rw5G001320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 632
Acc65I GGTACC 1 cut(s) 662
AccB1I GGYRCC 3 cut(s) 107, 538, 662
AciI CCGC 3 cut(s) 130, 401, 687
AclI AACGTT 1 cut(s) 144
AcoI YGGCCR 1 cut(s) 744
AcyI GRCGYC 2 cut(s) 108, 539
AfaI GTAC 2 cut(s) 482, 664
AfiI CCNNNNNNNGG 2 cut(s) 37, 100
AluBI AGCT 4 cut(s) 173, 349, 371, 467
AluI AGCT 4 cut(s) 173, 349, 371, 467
Alw26I GTCTC 1 cut(s) 666
Ama87I CYCGRG 1 cut(s) 632
AoxI GGCC 2 cut(s) 627, 744
ApeKI GCWGC 6 cut(s) 290, 293, 467, 680, 683, 689
Asp718I GGTACC 1 cut(s) 662
AspA2I CCTAGG 1 cut(s) 94
AspLEI GCGC 2 cut(s) 110, 541
AspS9I GGNCC 1 cut(s) 628
AsuC2I CCSGG 3 cut(s) 462, 529, 570
AsuII TTCGAA 1 cut(s) 212
AvaI CYCGRG 1 cut(s) 632
AvrII CCTAGG 1 cut(s) 94
BaeI ACNNNNGTAYC 2 cut(s) 537, 570
BanI GGYRCC 3 cut(s) 107, 538, 662
BbsI GAAGAC 1 cut(s) 647
BbvI GCAGC 6 cut(s) 277, 280, 454, 667, 670, 676
BccI CCATC 1 cut(s) 310
BceAI ACGGC 1 cut(s) 602
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BcnI CCSGG 3 cut(s) 462, 529, 570
BcoDI GTCTC 1 cut(s) 666
BfaI CTAG 3 cut(s) 95, 762, 769
BfoI RGCGCY 2 cut(s) 111, 542
BglI GCCNNNNNGGC 1 cut(s) 698
BisI GCNGC 7 cut(s) 291, 294, 468, 681, 684, 687, 690
BlnI CCTAGG 1 cut(s) 94
BlsI GCNGC 7 cut(s) 292, 295, 469, 682, 685, 688, 691
Bme1390I CCNGG 3 cut(s) 462, 529, 570
BmeT110I CYCGRG 1 cut(s) 632
BmgT120I GGNCC 1 cut(s) 628
BmiI GGNNCC 4 cut(s) 29, 109, 540, 664
BmrFI CCNGG 3 cut(s) 462, 529, 570
BmsI GCATC 7 cut(s) 78, 382, 404, 604, 700, 710, 744
BpiI GAAGAC 1 cut(s) 647
BpmI CTGGAG 1 cut(s) 690
Bpu14I TTCGAA 1 cut(s) 212
BpuEI CTTGAG 2 cut(s) 159, 371
BpuMI CCSGG 3 cut(s) 462, 529, 570
BsaHI GRCGYC 2 cut(s) 108, 539
BsaJI CCNNGG 5 cut(s) 31, 94, 471, 516, 528
BsaWI WCCGGW 1 cut(s) 725
BsaXI ACNNNNNCTCC 2 cut(s) 629, 659
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 100
Bse1I ACTGG 1 cut(s) 673
BseDI CCNNGG 5 cut(s) 31, 94, 471, 516, 528
BseGI GGATG 4 cut(s) 27, 397, 701, 759
BseLI CCNNNNNNNGG 2 cut(s) 37, 100
BseMII CTCAG 1 cut(s) 77
BseNI ACTGG 1 cut(s) 673
BseRI GAGGAG 2 cut(s) 115, 650
BseXI GCAGC 6 cut(s) 277, 280, 454, 667, 670, 676
BsgI GTGCAG 1 cut(s) 272
BshFI GGCC 2 cut(s) 629, 746
BshNI GGYRCC 3 cut(s) 107, 538, 662
BsiHKCI CYCGRG 1 cut(s) 632
BsiSI CCGG 5 cut(s) 461, 528, 569, 726, 747
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 2 cut(s) 37, 100
BsmAI GTCTC 1 cut(s) 666
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 2 cut(s) 629, 746
BsoBI CYCGRG 1 cut(s) 632
Bsp119I TTCGAA 1 cut(s) 212
Bsp19I CCATGG 1 cut(s) 471
BspACI CCGC 3 cut(s) 130, 401, 687
BspANI GGCC 2 cut(s) 629, 746
BspCNI CTCAG 1 cut(s) 76
BspLI GGNNCC 4 cut(s) 29, 109, 540, 664
BspT104I TTCGAA 1 cut(s) 212
BspT107I GGYRCC 3 cut(s) 107, 538, 662
BsrI ACTGG 1 cut(s) 673
BssECI CCNNGG 5 cut(s) 31, 94, 471, 516, 528
BssNI GRCGYC 2 cut(s) 108, 539
BssT1I CCWWGG 4 cut(s) 31, 94, 471, 516
Bst4CI ACNGT 2 cut(s) 250, 499
BstACI GRCGYC 2 cut(s) 108, 539
BstBI TTCGAA 1 cut(s) 212
BstC8I GCNNGC 2 cut(s) 134, 448
BstDEI CTNAG 1 cut(s) 63
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 4 cut(s) 27, 397, 701, 759
BstH2I RGCGCY 2 cut(s) 111, 542
BstHHI GCGC 2 cut(s) 110, 541
BstMAI GTCTC 1 cut(s) 666
BstMWI GCNNNNNNNGC 5 cut(s) 346, 473, 686, 689, 698
BstSCI CCNGG 3 cut(s) 460, 527, 568
BstV1I GCAGC 6 cut(s) 277, 280, 454, 667, 670, 676
BstV2I GAAGAC 1 cut(s) 647
BstXI CCANNNNNNTGG 1 cut(s) 742
BsuRI GGCC 2 cut(s) 629, 746
BtgI CCRYGG 1 cut(s) 471
BtsCI GGATG 4 cut(s) 27, 397, 701, 759
BtsIMutI CAGTG 1 cut(s) 666
Cac8I GCNNGC 2 cut(s) 134, 448
CfoI GCGC 2 cut(s) 110, 541
Cfr13I GGNCC 1 cut(s) 628
Csp6I GTAC 2 cut(s) 481, 663
CviAII CATG 3 cut(s) 472, 535, 676
CviQI GTAC 2 cut(s) 481, 663
DdeI CTNAG 1 cut(s) 63
DinI GGCGCC 2 cut(s) 109, 540
EaeI YGGCCR 1 cut(s) 744
Eco130I CCWWGG 4 cut(s) 31, 94, 471, 516
Eco88I CYCGRG 1 cut(s) 632
EcoO109I RGGNCCY 1 cut(s) 628
EcoT14I CCWWGG 4 cut(s) 31, 94, 471, 516
EcoT22I ATGCAT 1 cut(s) 397
EgeI GGCGCC 2 cut(s) 109, 540
EheI GGCGCC 2 cut(s) 109, 540
ErhI CCWWGG 4 cut(s) 31, 94, 471, 516
FaeI CATG 3 cut(s) 475, 538, 679
FaqI GGGAC 1 cut(s) 545
FatI CATG 3 cut(s) 471, 534, 675
FauI CCCGC 1 cut(s) 137
Fnu4HI GCNGC 7 cut(s) 291, 294, 468, 681, 684, 687, 690
FokI GGATG 4 cut(s) 34, 404, 688, 766
Fsp4HI GCNGC 7 cut(s) 291, 294, 468, 681, 684, 687, 690
FspBI CTAG 3 cut(s) 95, 762, 769
GlaI GCGC 2 cut(s) 109, 540
GluI GCNGC 7 cut(s) 291, 294, 468, 681, 684, 687, 690
GsuI CTGGAG 1 cut(s) 690
HaeII RGCGCY 2 cut(s) 111, 542
HaeIII GGCC 2 cut(s) 629, 746
HapII CCGG 5 cut(s) 461, 528, 569, 726, 747
HhaI GCGC 2 cut(s) 110, 541
Hin1I GRCGYC 2 cut(s) 108, 539
Hin1II CATG 3 cut(s) 475, 538, 679
Hin6I GCGC 2 cut(s) 108, 539
HinP1I GCGC 2 cut(s) 108, 539
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 4 cut(s) 195, 232, 332, 765
HpaII CCGG 5 cut(s) 461, 528, 569, 726, 747
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 1 cut(s) 706
Hpy188III TCNNGA 1 cut(s) 762
Hpy8I GTNNAC 1 cut(s) 310
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 4 cut(s) 98, 406, 512, 619
HpyCH4III ACNGT 2 cut(s) 250, 499
HpyCH4IV ACGT 3 cut(s) 144, 207, 644
HpyCH4V TGCA 3 cut(s) 253, 322, 395
HpyF10VI GCNNNNNNNGC 5 cut(s) 346, 473, 686, 689, 698
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 3 cut(s) 144, 207, 644
Hsp92I GRCGYC 2 cut(s) 108, 539
Hsp92II CATG 3 cut(s) 475, 538, 679
HspAI GCGC 2 cut(s) 108, 539
KasI GGCGCC 2 cut(s) 107, 538
KpnI GGTACC 1 cut(s) 666
LmnI GCTCC 2 cut(s) 464, 571
Lsp1109I GCAGC 6 cut(s) 277, 280, 454, 667, 670, 676
LweI GCATC 7 cut(s) 78, 382, 404, 604, 700, 710, 744
MaeI CTAG 3 cut(s) 95, 762, 769
MaeII ACGT 3 cut(s) 144, 207, 644
MaeIII GTNAC 3 cut(s) 49, 487, 645
MboII GAAGA 4 cut(s) 25, 241, 411, 652
MfeI CAATTG 1 cut(s) 441
MluCI AATT 3 cut(s) 180, 441, 556
Mly113I GGCGCC 2 cut(s) 108, 539
MlyI GAGTC 2 cut(s) 204, 759
Mph1103I ATGCAT 1 cut(s) 397
MseI TTAA 1 cut(s) 168
MslI CAYNNNNRTG 2 cut(s) 476, 740
MspA1I CMGCKG 1 cut(s) 689
MspI CCGG 5 cut(s) 461, 528, 569, 726, 747
MspR9I CCNGG 3 cut(s) 462, 529, 570
MunI CAATTG 1 cut(s) 441
MwoI GCNNNNNNNGC 5 cut(s) 346, 473, 686, 689, 698
NarI GGCGCC 2 cut(s) 108, 539
NciI CCSGG 3 cut(s) 462, 529, 570
NcoI CCATGG 1 cut(s) 471
NlaIII CATG 3 cut(s) 475, 538, 679
NlaIV GGNNCC 4 cut(s) 29, 109, 540, 664
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 2 cut(s) 49, 487
NsiI ATGCAT 1 cut(s) 397
NspV TTCGAA 1 cut(s) 212
PaeR7I CTCGAG 1 cut(s) 632
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 204
PfeI GAWTC 2 cut(s) 232, 332
PkrI GCNGC 7 cut(s) 292, 295, 469, 682, 685, 688, 691
PleI GAGTC 2 cut(s) 203, 759
PluTI GGCGCC 2 cut(s) 111, 542
PpsI GAGTC 2 cut(s) 203, 759
Psp1406I AACGTT 1 cut(s) 144
PspN4I GGNNCC 4 cut(s) 29, 109, 540, 664
PspPI GGNCC 1 cut(s) 628
PspXI VCTCGAGB 1 cut(s) 632
RsaI GTAC 2 cut(s) 482, 664
RsaNI GTAC 2 cut(s) 481, 663
RseI CAYNNNNRTG 2 cut(s) 476, 740
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 7 cut(s) 291, 294, 468, 681, 684, 687, 690
Sau96I GGNCC 1 cut(s) 628
SchI GAGTC 2 cut(s) 204, 759
ScrFI CCNGG 3 cut(s) 462, 529, 570
SfaNI GCATC 7 cut(s) 78, 382, 404, 604, 700, 710, 744
SfoI GGCGCC 2 cut(s) 109, 540
Sfr274I CTCGAG 1 cut(s) 632
SfuI TTCGAA 1 cut(s) 212
SlaI CTCGAG 1 cut(s) 632
SmiMI CAYNNNNRTG 2 cut(s) 476, 740
SmlI CTYRAG 3 cut(s) 174, 386, 632
SmoI CTYRAG 3 cut(s) 174, 386, 632
Sse9I AATT 3 cut(s) 180, 441, 556
SsiI CCGC 3 cut(s) 130, 401, 687
SspDI GGCGCC 2 cut(s) 107, 538
SspMI CTAG 3 cut(s) 95, 762, 769
StyD4I CCNGG 3 cut(s) 460, 527, 568
StyI CCWWGG 4 cut(s) 31, 94, 471, 516
TaaI ACNGT 2 cut(s) 250, 499
TaiI ACGT 3 cut(s) 147, 210, 647
TaqI TCGA 3 cut(s) 11, 212, 633
TasI AATT 3 cut(s) 180, 441, 556
TauI GCSGC 1 cut(s) 689
TfiI GAWTC 2 cut(s) 232, 332
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TscAI CASTG 1 cut(s) 673
TseFI GTSAC 2 cut(s) 49, 487
TseI GCWGC 6 cut(s) 290, 293, 467, 680, 683, 689
Tsp45I GTSAC 2 cut(s) 49, 487
TspDTI ATGAA 2 cut(s) 165, 585
TspGWI ACGGA 2 cut(s) 103, 276
TspRI CASTG 1 cut(s) 673
XbaI TCTAGA 1 cut(s) 761
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XhoI CTCGAG 1 cut(s) 632
XmaJI CCTAGG 1 cut(s) 94
XspI CTAG 3 cut(s) 95, 762, 769
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.