AT4G10100

Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014109
Physical Location & Seq
Forward (+)
6308683 .. 6309546
864 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G10100.1

Sequence Viewer

Length: 291 bp
ATGGACAAAGAAGTTACAAAGATTGAAAGTGATGACACTTCATCGGTGGAGATCAAAGTGTTGTTATTCGCCAGAGCACGAGAGCTCACAGGTGTGCCTGATCTAACACTGAAGATGCCATCAGGTAGTACAACACAGAAATGCCTGGATGAGTTGGTGCTTAAGTTTCCAAGCTTGGAAGAGGTACGTAGCTGTGTTGTTCTCGCTTTGAACGAGGAATATACAACCGATTCCGCCATTGTTCAACATAGAGATGAGTTAGCCATCATACCTCCGATAAGCGGCGGCTAA

Protein Analysis

96

Amino Acids

10.53

Weight (kDa)

4.57

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiS PF02597 20 - 96 3.3e-16 ThiS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014109)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10100 AT4G10100 AT4G10100
fragaria_vesca FvH4_2g00750
malus_domestica MD05G1003200.v1.1 MD10G1004200.v1.1
prunus_persica Prupe.8G006600_v2.0.a1
pyrus_communis pycom05g00290
rosa_chinensis RchiOBHm_Chr6g0251131
rosa_laevigata RLG00000015481
rosa_roxburghii Rroxscaffold_7G00217010
rosa_rugosa Rorug05G0500600
rosa_samantha Rh6AG009500 Rh6BG002400 Rh6CG006300 Rh6DG006600
rosa_wichuraiana Rw6G000950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 234, 282, 285
AcuI CTGAAG 1 cut(s) 131
AfaI GTAC 2 cut(s) 130, 186
AfiI CCNNNNNNNGG 1 cut(s) 281
AflII CTTAAG 1 cut(s) 161
AgsI TTSAA 3 cut(s) 26, 211, 245
AjnI CCWGG 1 cut(s) 144
AleI CACNNNNGTG 1 cut(s) 92
AluBI AGCT 3 cut(s) 85, 174, 192
AluI AGCT 3 cut(s) 85, 174, 192
Alw21I GWGCWC 2 cut(s) 79, 87
BanII GRGCYC 1 cut(s) 87
BauI CACGAG 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 79, 87
BccI CCATC 2 cut(s) 127, 272
BciT130I CCWGG 1 cut(s) 146
BfrI CTTAAG 1 cut(s) 161
BisI GCNGC 2 cut(s) 283, 286
BlsI GCNGC 2 cut(s) 284, 287
Bme1390I CCNGG 1 cut(s) 146
BmrFI CCNGG 1 cut(s) 146
BmsI GCATC 1 cut(s) 105
BsaAI YACGTR 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 281
BseBI CCWGG 1 cut(s) 146
BseGI GGATG 1 cut(s) 154
BseLI CCNNNNNNNGG 1 cut(s) 281
BsiHKAI GWGCWC 2 cut(s) 79, 87
BslI CCNNNNNNNGG 1 cut(s) 281
Bsp1286I GDGCHC 2 cut(s) 79, 87
Bsp143I GATC 2 cut(s) 51, 100
BspACI CCGC 3 cut(s) 234, 282, 285
BspTI CTTAAG 1 cut(s) 161
BssMI GATC 2 cut(s) 51, 100
BssSI CACGAG 1 cut(s) 78
Bst2BI CACGAG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 146
Bst6I CTCTTC 1 cut(s) 174
BstAFI CTTAAG 1 cut(s) 161
BstBAI YACGTR 1 cut(s) 188
BstF5I GGATG 1 cut(s) 154
BstKTI GATC 2 cut(s) 54, 103
BstMBI GATC 2 cut(s) 51, 100
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 144
BstSNI TACGTA 1 cut(s) 188
BtsCI GGATG 1 cut(s) 154
BtsIMutI CAGTG 1 cut(s) 107
Csp6I GTAC 2 cut(s) 129, 185
CviJI RGCY 5 cut(s) 85, 174, 192, 263, 288
CviKI_1 RGCY 5 cut(s) 85, 174, 192, 263, 288
CviQI GTAC 2 cut(s) 129, 185
DpnI GATC 2 cut(s) 53, 102
DpnII GATC 2 cut(s) 51, 100
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
EciI GGCGGA 1 cut(s) 223
Ecl136II GAGCTC 1 cut(s) 85
Eco105I TACGTA 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 131
EcoICRI GAGCTC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 144
EcoT38I GRGCYC 1 cut(s) 87
FaiI YATR 3 cut(s) 222, 249, 269
Fnu4HI GCNGC 2 cut(s) 283, 286
FokI GGATG 1 cut(s) 161
FriOI GRGCYC 1 cut(s) 87
Fsp4HI GCNGC 2 cut(s) 283, 286
GluI GCNGC 2 cut(s) 283, 286
HindIII AAGCTT 1 cut(s) 172
HinfI GANTC 1 cut(s) 230
Hpy188I TCNGA 1 cut(s) 276
HpyCH4IV ACGT 1 cut(s) 187
HpySE526I ACGT 1 cut(s) 187
Kzo9I GATC 2 cut(s) 51, 100
LpnPI CCDG 6 cut(s) 75, 85, 108, 111, 131, 158
LweI GCATC 1 cut(s) 105
MaeII ACGT 1 cut(s) 187
MaeIII GTNAC 1 cut(s) 13
MalI GATC 2 cut(s) 53, 102
MboI GATC 2 cut(s) 51, 100
MboII GAAGA 2 cut(s) 124, 191
MhlI GDGCHC 2 cut(s) 79, 87
MnlI CCTC 3 cut(s) 175, 208, 282
MseI TTAA 1 cut(s) 162
MslI CAYNNNNRTG 3 cut(s) 92, 139, 252
MspCI CTTAAG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 146
MvaI CCWGG 1 cut(s) 146
NdeII GATC 2 cut(s) 51, 100
OliI CACNNNNGTG 1 cut(s) 92
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 1 cut(s) 230
PkrI GCNGC 2 cut(s) 284, 287
Ppu21I YACGTR 1 cut(s) 188
Psp124BI GAGCTC 1 cut(s) 87
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
RsaI GTAC 2 cut(s) 130, 186
RsaNI GTAC 2 cut(s) 129, 185
RseI CAYNNNNRTG 3 cut(s) 92, 139, 252
SacI GAGCTC 1 cut(s) 87
SaqAI TTAA 1 cut(s) 162
SatI GCNGC 2 cut(s) 283, 286
Sau3AI GATC 2 cut(s) 51, 100
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 2 cut(s) 79, 87
SetI ASST 8 cut(s) 87, 94, 127, 176, 186, 190, 194, 274
SfaNI GCATC 1 cut(s) 105
SmiMI CAYNNNNRTG 3 cut(s) 92, 139, 252
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
SnaBI TACGTA 1 cut(s) 188
SsiI CCGC 3 cut(s) 234, 282, 285
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 144
TaiI ACGT 1 cut(s) 190
TatI WGTACW 1 cut(s) 128
TauI GCSGC 2 cut(s) 285, 288
TfiI GAWTC 1 cut(s) 230
Tru1I TTAA 1 cut(s) 162
Tru9I TTAA 1 cut(s) 162
TscAI CASTG 1 cut(s) 114
TspDTI ATGAA 1 cut(s) 30
TspRI CASTG 1 cut(s) 114
Vha464I CTTAAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.