Rh6CG006300

Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0014109
Physical Location & Seq
Forward (+)
795586 .. 799000
3415 bp
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UTR
Exon/CDS
Intron
Rh6CG006300.1

Sequence Viewer

Length: 318 bp
ATGGATTTCAAGCAAGAAGAGATTAAGTCTGTAACTATGGACCCTACAACTAAGGGGATCAAGGATTCATCTGTTCAGATGAAGGTGTTGTTCTTTGCCAGGGCTCGGGACCTCACTGGCCTCACAGAGATTCCATTGGAGGTATCATCTGGCAGTACAGCCGATGATTGTTTAAATAAGCTCATTTCCATTTTTCCAAGCTTGGAAGAGATCCGTGGGTGCATGGTTCTTGCTCTTAACGAAGAGTACACAACTGAGTCGGCACTTGTTAAAGACAAGGATGAGTTGGCCATTATACCTCCAATCAGTGGGGGCTAA

Protein Analysis

105

Amino Acids

11.48

Weight (kDa)

4.49

Isoelectric Point (pI)

41.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiS PF02597 29 - 105 3.5e-14 ThiS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014109)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10100 AT4G10100 AT4G10100
fragaria_vesca FvH4_2g00750
malus_domestica MD05G1003200.v1.1 MD10G1004200.v1.1
prunus_persica Prupe.8G006600_v2.0.a1
pyrus_communis pycom05g00290
rosa_chinensis RchiOBHm_Chr6g0251131
rosa_laevigata RLG00000015481
rosa_roxburghii Rroxscaffold_7G00217010
rosa_rugosa Rorug05G0500600
rosa_samantha Rh6AG009500 Rh6BG002400 Rh6CG006300 Rh6DG006600
rosa_wichuraiana Rw6G000950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 308
AclWI GGATC 2 cut(s) 65, 205
AcoI YGGCCR 1 cut(s) 288
AfaI GTAC 2 cut(s) 157, 248
AfiI CCNNNNNNNGG 2 cut(s) 105, 308
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 1 cut(s) 98
AluBI AGCT 2 cut(s) 181, 201
AluI AGCT 2 cut(s) 181, 201
AlwI GGATC 2 cut(s) 65, 205
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 2 cut(s) 118, 288
AspS9I GGNCC 2 cut(s) 40, 109
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 2 cut(s) 40, 109
BalI TGGCCA 1 cut(s) 290
BanII GRGCYC 1 cut(s) 106
BciT130I CCWGG 1 cut(s) 100
Bme1390I CCNGG 1 cut(s) 100
Bme18I GGWCC 2 cut(s) 40, 109
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 2 cut(s) 40, 109
BmiI GGNNCC 2 cut(s) 42, 110
BmrFI CCNGG 1 cut(s) 100
BsaJI CCNNGG 2 cut(s) 99, 214
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 308
Bse1I ACTGG 1 cut(s) 121
BseBI CCWGG 1 cut(s) 100
BseDI CCNNGG 2 cut(s) 99, 214
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 2 cut(s) 105, 308
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 1 cut(s) 121
BshFI GGCC 2 cut(s) 120, 290
BsiHKCI CYCGRG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 105, 308
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 2 cut(s) 120, 290
BsoBI CYCGRG 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 2 cut(s) 57, 210
BspANI GGCC 2 cut(s) 120, 290
BspCNI CTCAG 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 42, 110
BspPI GGATC 2 cut(s) 65, 205
BsrI ACTGG 1 cut(s) 121
BssECI CCNNGG 2 cut(s) 99, 214
BssMI GATC 2 cut(s) 57, 210
Bst2UI CCWGG 1 cut(s) 100
Bst6I CTCTTC 3 cut(s) 12, 201, 237
BstDEI CTNAG 2 cut(s) 51, 255
BstDSI CCRYGG 1 cut(s) 214
BstF5I GGATG 1 cut(s) 286
BstKTI GATC 2 cut(s) 60, 213
BstMBI GATC 2 cut(s) 57, 210
BstNI CCWGG 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
BsuRI GGCC 2 cut(s) 120, 290
BtgI CCRYGG 1 cut(s) 214
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 2 cut(s) 114, 313
Cfr13I GGNCC 2 cut(s) 40, 109
Csp6I GTAC 2 cut(s) 156, 247
CviAII CATG 1 cut(s) 223
CviJI RGCY 7 cut(s) 104, 120, 161, 181, 201, 290, 315
CviKI_1 RGCY 7 cut(s) 104, 120, 161, 181, 201, 290, 315
CviQI GTAC 2 cut(s) 156, 247
DdeI CTNAG 2 cut(s) 51, 255
DpnI GATC 2 cut(s) 59, 212
DpnII GATC 2 cut(s) 57, 210
DraI TTTAAA 1 cut(s) 174
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 3 cut(s) 12, 201, 237
EarI CTCTTC 3 cut(s) 12, 201, 237
Eco24I GRGCYC 1 cut(s) 106
Eco47I GGWCC 2 cut(s) 40, 109
Eco88I CYCGRG 1 cut(s) 105
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 106
FaeI CATG 1 cut(s) 226
FaiI YATR 3 cut(s) 38, 224, 296
FaqI GGGAC 1 cut(s) 122
FatI CATG 1 cut(s) 222
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 1 cut(s) 106
HaeIII GGCC 2 cut(s) 120, 290
Hin1II CATG 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 3 cut(s) 65, 130, 257
Hpy166II GTNNAC 1 cut(s) 249
Hpy188I TCNGA 1 cut(s) 78
Hpy188III TCNNGA 1 cut(s) 107
Hpy8I GTNNAC 1 cut(s) 249
HpyAV CCTTC 1 cut(s) 76
HpyCH4V TGCA 1 cut(s) 222
HpyF3I CTNAG 2 cut(s) 51, 255
Hsp92II CATG 1 cut(s) 226
Kzo9I GATC 2 cut(s) 57, 210
LpnPI CCDG 4 cut(s) 85, 102, 112, 135
MaeIII GTNAC 1 cut(s) 31
MalI GATC 2 cut(s) 59, 212
MboI GATC 2 cut(s) 57, 210
MboII GAAGA 3 cut(s) 29, 218, 254
MflI RGATCY 1 cut(s) 210
MhlI GDGCHC 1 cut(s) 106
MlsI TGGCCA 1 cut(s) 290
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 1 cut(s) 266
MnlI CCTC 4 cut(s) 122, 131, 133, 309
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
MseI TTAA 4 cut(s) 24, 173, 237, 270
Msp20I TGGCCA 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 100
MvaI CCWGG 1 cut(s) 100
NdeII GATC 2 cut(s) 57, 210
NlaIII CATG 1 cut(s) 226
NlaIV GGNNCC 2 cut(s) 42, 110
PfeI GAWTC 2 cut(s) 65, 130
PflMI CCANNNNNTGG 1 cut(s) 308
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 98
PspGI CCWGG 1 cut(s) 98
PspN4I GGNNCC 2 cut(s) 42, 110
PspPI GGNCC 2 cut(s) 40, 109
PspPPI RGGWCCY 1 cut(s) 109
PsuI RGATCY 1 cut(s) 210
RsaI GTAC 2 cut(s) 157, 248
RsaNI GTAC 2 cut(s) 156, 247
SaqAI TTAA 4 cut(s) 24, 173, 237, 270
Sau3AI GATC 2 cut(s) 57, 210
Sau96I GGNCC 2 cut(s) 40, 109
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 1 cut(s) 100
SduI GDGCHC 1 cut(s) 106
SetI ASST 6 cut(s) 87, 114, 144, 183, 203, 301
SinI GGWCC 2 cut(s) 40, 109
StyD4I CCNGG 1 cut(s) 98
TatI WGTACW 2 cut(s) 155, 246
TfiI GAWTC 2 cut(s) 65, 130
Tru1I TTAA 4 cut(s) 24, 173, 237, 270
Tru9I TTAA 4 cut(s) 24, 173, 237, 270
TscAI CASTG 2 cut(s) 121, 313
TspDTI ATGAA 2 cut(s) 57, 95
TspGWI ACGGA 1 cut(s) 203
TspRI CASTG 2 cut(s) 121, 313
Van91I CCANNNNNTGG 1 cut(s) 308
VpaK11BI GGWCC 2 cut(s) 40, 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.