MD05G1003200.v1.1

Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014109
Physical Location & Seq
Forward (+)
1104594 .. 1106974
2381 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1003200.v1.1.491

Sequence Viewer

Length: 318 bp
ATGGATTTCAAACAAGAAGAGAGTAAGACGGTAACCATGGACTCTACAAGTCAGGGGATTGAGGGTTCATCTGTTAAGATAAAGGTGTTGTTCTTTGCCAGGGCTCGTGGCCTAACTGGCTTGAGTGATATGCCACTGGAGGTGTCCTCTGGTAGTACTGCCCTTGATTGTTTAGATAAGCTCATCGCCATGTTTCCAAGCTTGACAGAGATCCGCGGATGCATGGTGCTTGCTCTTAATGAGGCGTACACAGCTGATTCTGCAGTCGTCAAAGACAAGGATGAGTTGGCCATAATACCTCCTATAAGTGGTGGCTGA

Protein Analysis

106

Amino Acids

11.16

Weight (kDa)

4.56

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiS PF02597 29 - 105 8.6e-13 ThiS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014109)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10100 AT4G10100 AT4G10100
fragaria_vesca FvH4_2g00750
malus_domestica MD05G1003200.v1.1 MD10G1004200.v1.1
prunus_persica Prupe.8G006600_v2.0.a1
pyrus_communis pycom05g00290
rosa_chinensis RchiOBHm_Chr6g0251131
rosa_laevigata RLG00000015481
rosa_roxburghii Rroxscaffold_7G00217010
rosa_rugosa Rorug05G0500600
rosa_samantha Rh6AG009500 Rh6BG002400 Rh6CG006300 Rh6DG006600
rosa_wichuraiana Rw6G000950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 216
AciI CCGC 2 cut(s) 214, 216
AclWI GGATC 1 cut(s) 205
AcoI YGGCCR 1 cut(s) 288
AfaI GTAC 2 cut(s) 157, 248
AfiI CCNNNNNNNGG 1 cut(s) 308
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 1 cut(s) 98
AloI GAACNNNNNNTCC 2 cut(s) 49, 81
AluBI AGCT 3 cut(s) 181, 201, 254
AluI AGCT 3 cut(s) 181, 201, 254
AlwI GGATC 1 cut(s) 205
AoxI GGCC 2 cut(s) 109, 288
BalI TGGCCA 1 cut(s) 290
BanII GRGCYC 1 cut(s) 106
BauI CACGAG 1 cut(s) 105
BciT130I CCWGG 1 cut(s) 100
BfmI CTRYAG 1 cut(s) 261
BglI GCCNNNNNGGC 1 cut(s) 117
BmcAI AGTACT 1 cut(s) 157
Bme1390I CCNGG 1 cut(s) 100
BmrFI CCNGG 1 cut(s) 100
BmsI GCATC 1 cut(s) 209
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpmI CTGGAG 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 142
BsaJI CCNNGG 3 cut(s) 36, 99, 214
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse1I ACTGG 2 cut(s) 121, 141
BseBI CCWGG 1 cut(s) 100
BseDI CCNNGG 3 cut(s) 36, 99, 214
BseGI GGATG 2 cut(s) 224, 286
BseLI CCNNNNNNNGG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 121, 141
Bsh1236I CGCG 1 cut(s) 216
BshFI GGCC 2 cut(s) 111, 290
BslI CCNNNNNNNGG 1 cut(s) 308
BsnI GGCC 2 cut(s) 111, 290
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 1 cut(s) 210
Bsp19I CCATGG 1 cut(s) 36
BspACI CCGC 2 cut(s) 214, 216
BspANI GGCC 2 cut(s) 111, 290
BspFNI CGCG 1 cut(s) 216
BspMAI CTGCAG 1 cut(s) 265
BspPI GGATC 1 cut(s) 205
BsrI ACTGG 2 cut(s) 121, 141
BssECI CCNNGG 3 cut(s) 36, 99, 214
BssMI GATC 1 cut(s) 210
BssSI CACGAG 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 36
Bst2BI CACGAG 1 cut(s) 105
Bst2UI CCWGG 1 cut(s) 100
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 12
BstC8I GCNNGC 1 cut(s) 231
BstDSI CCRYGG 2 cut(s) 36, 214
BstEII GGTNACC 1 cut(s) 31
BstF5I GGATG 2 cut(s) 224, 286
BstFNI CGCG 1 cut(s) 216
BstKTI GATC 1 cut(s) 213
BstMBI GATC 1 cut(s) 210
BstMWI GCNNNNNNNGC 3 cut(s) 117, 251, 260
BstNI CCWGG 1 cut(s) 100
BstPI GGTNACC 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 98
BstSFI CTRYAG 1 cut(s) 261
BstUI CGCG 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
BsuRI GGCC 2 cut(s) 111, 290
BtgI CCRYGG 2 cut(s) 36, 214
BtgZI GCGATG 1 cut(s) 169
BtsCI GGATG 2 cut(s) 224, 286
BtsIMutI CAGTG 1 cut(s) 134
Cac8I GCNNGC 1 cut(s) 231
Cfr42I CCGCGG 1 cut(s) 217
Csp6I GTAC 2 cut(s) 156, 247
CviAII CATG 3 cut(s) 37, 190, 223
CviJI RGCY 8 cut(s) 104, 111, 120, 181, 201, 254, 290, 315
CviKI_1 RGCY 8 cut(s) 104, 111, 120, 181, 201, 254, 290, 315
CviQI GTAC 2 cut(s) 156, 247
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 106
Eco91I GGTNACC 1 cut(s) 31
EcoO65I GGTNACC 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 36
EcoT22I ATGCAT 1 cut(s) 224
EcoT38I GRGCYC 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 3 cut(s) 40, 193, 226
FaiI YATR 6 cut(s) 38, 131, 191, 224, 293, 305
FatI CATG 3 cut(s) 36, 189, 222
FokI GGATG 2 cut(s) 231, 293
FriOI GRGCYC 1 cut(s) 106
GsuI CTGGAG 1 cut(s) 158
HaeIII GGCC 2 cut(s) 111, 290
Hin1II CATG 3 cut(s) 40, 193, 226
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 2 cut(s) 41, 257
Hpy166II GTNNAC 1 cut(s) 249
Hpy8I GTNNAC 1 cut(s) 249
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 2 cut(s) 222, 263
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 251, 260
Hsp92II CATG 3 cut(s) 40, 193, 226
KspI CCGCGG 1 cut(s) 217
Kzo9I GATC 1 cut(s) 210
LpnPI CCDG 6 cut(s) 38, 85, 102, 112, 122, 135
LweI GCATC 1 cut(s) 209
MaeIII GTNAC 1 cut(s) 31
MalI GATC 1 cut(s) 212
MboI GATC 1 cut(s) 210
MboII GAAGA 1 cut(s) 29
MflI RGATCY 1 cut(s) 210
MhlI GDGCHC 1 cut(s) 106
MlsI TGGCCA 1 cut(s) 290
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 5 cut(s) 55, 133, 157, 235, 309
Mox20I TGGCCA 1 cut(s) 290
Mph1103I ATGCAT 1 cut(s) 224
MscI TGGCCA 1 cut(s) 290
MseI TTAA 2 cut(s) 75, 237
MslI CAYNNNNRTG 1 cut(s) 188
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 2 cut(s) 216, 254
MspR9I CCNGG 1 cut(s) 100
MvaI CCWGG 1 cut(s) 100
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 3 cut(s) 117, 251, 260
NcoI CCATGG 1 cut(s) 36
NdeII GATC 1 cut(s) 210
NlaIII CATG 3 cut(s) 40, 193, 226
NsiI ATGCAT 1 cut(s) 224
PfeI GAWTC 1 cut(s) 257
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 98
PspEI GGTNACC 1 cut(s) 31
PspGI CCWGG 1 cut(s) 98
PstI CTGCAG 1 cut(s) 265
PsuI RGATCY 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 2 cut(s) 157, 248
RsaNI GTAC 2 cut(s) 156, 247
RseI CAYNNNNRTG 1 cut(s) 188
SacII CCGCGG 1 cut(s) 217
SaqAI TTAA 2 cut(s) 75, 237
Sau3AI GATC 1 cut(s) 210
ScaI AGTACT 1 cut(s) 157
SchI GAGTC 1 cut(s) 35
ScrFI CCNGG 1 cut(s) 100
SduI GDGCHC 1 cut(s) 106
SetI ASST 6 cut(s) 87, 144, 183, 203, 256, 301
SfaNI GCATC 1 cut(s) 209
SfcI CTRYAG 1 cut(s) 261
Sfr303I CCGCGG 1 cut(s) 217
SgrBI CCGCGG 1 cut(s) 217
SmiMI CAYNNNNRTG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
SsiI CCGC 2 cut(s) 214, 216
StyD4I CCNGG 1 cut(s) 98
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 31
TatI WGTACW 1 cut(s) 155
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 2 cut(s) 75, 237
Tru9I TTAA 2 cut(s) 75, 237
TscAI CASTG 1 cut(s) 141
TspDTI ATGAA 1 cut(s) 57
TspRI CASTG 1 cut(s) 141
ZrmI AGTACT 1 cut(s) 157
Zsp2I ATGCAT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.