FvH4_2g00750

Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014109
Physical Location & Seq
Forward (+)
771558 .. 782575
11018 bp
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UTR
Exon/CDS
Intron
FvH4_2g00750.t1

Sequence Viewer

Length: 318 bp
ATGGATTTCAAGCAAGAAGAAATTAATTCCGTAACTATGGACCCTAAAACTAAGAGGATTCAGGATTCGTCTGTTAAGATGAAGGTGTTGTTTTTTGCCAGGGCTCGGGACCTTACTGGCTTCACTGAGATGCCACTGGAGGTATCATCTGGTAGTACAGCCAATGATTGTTTAAATAAGCTCATTTCCATTTTCCCTAGCTTGGAAGAGATCAGGGGGTGCATGGTGCTTGCTCTTAATGAAGAGTACACAACTGAGTCGGCAGTTGTGCAAGACAAAGATGAGTTGGCCATCATACCTCCAATCAGTGGTGGCTAA

Protein Analysis

106

Amino Acids

11.63

Weight (kDa)

4.57

Isoelectric Point (pI)

57.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiS PF02597 29 - 105 1.5e-14 ThiS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014109)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10100 AT4G10100 AT4G10100
fragaria_vesca FvH4_2g00750
malus_domestica MD05G1003200.v1.1 MD10G1004200.v1.1
prunus_persica Prupe.8G006600_v2.0.a1
pyrus_communis pycom05g00290
rosa_chinensis RchiOBHm_Chr6g0251131
rosa_laevigata RLG00000015481
rosa_roxburghii Rroxscaffold_7G00217010
rosa_rugosa Rorug05G0500600
rosa_samantha Rh6AG009500 Rh6BG002400 Rh6CG006300 Rh6DG006600
rosa_wichuraiana Rw6G000950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 308
AcoI YGGCCR 1 cut(s) 288
AfaI GTAC 2 cut(s) 157, 248
AfiI CCNNNNNNNGG 3 cut(s) 105, 202, 308
AgsI TTSAA 1 cut(s) 10
AjnI CCWGG 1 cut(s) 98
AluBI AGCT 2 cut(s) 181, 201
AluI AGCT 2 cut(s) 181, 201
Ama87I CYCGRG 1 cut(s) 105
AoxI GGCC 1 cut(s) 288
AseI ATTAAT 1 cut(s) 24
AspS9I GGNCC 2 cut(s) 40, 109
AvaI CYCGRG 1 cut(s) 105
AvaII GGWCC 2 cut(s) 40, 109
BalI TGGCCA 1 cut(s) 290
BanII GRGCYC 1 cut(s) 106
BccI CCATC 1 cut(s) 299
BciT130I CCWGG 1 cut(s) 100
BfaI CTAG 1 cut(s) 198
Bme1390I CCNGG 1 cut(s) 100
Bme18I GGWCC 2 cut(s) 40, 109
BmeT110I CYCGRG 1 cut(s) 105
BmgT120I GGNCC 2 cut(s) 40, 109
BmiI GGNNCC 2 cut(s) 42, 110
BmrFI CCNGG 1 cut(s) 100
BmsI GCATC 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 99
Bsc4I CCNNNNNNNGG 3 cut(s) 105, 202, 308
Bse1I ACTGG 2 cut(s) 121, 141
BseBI CCWGG 1 cut(s) 100
BseDI CCNNGG 1 cut(s) 99
BseLI CCNNNNNNNGG 3 cut(s) 105, 202, 308
BseMII CTCAG 2 cut(s) 117, 246
BseNI ACTGG 2 cut(s) 121, 141
BshFI GGCC 1 cut(s) 290
BsiHKCI CYCGRG 1 cut(s) 105
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 3 cut(s) 105, 202, 308
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 1 cut(s) 290
BsoBI CYCGRG 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 1 cut(s) 210
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 2 cut(s) 118, 247
BspLI GGNNCC 2 cut(s) 42, 110
BsrI ACTGG 2 cut(s) 121, 141
BssECI CCNNGG 1 cut(s) 99
BssMI GATC 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 100
Bst6I CTCTTC 2 cut(s) 201, 237
BstC8I GCNNGC 1 cut(s) 231
BstDEI CTNAG 3 cut(s) 51, 126, 255
BstKTI GATC 1 cut(s) 213
BstMBI GATC 1 cut(s) 210
BstNI CCWGG 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 98
BsuRI GGCC 1 cut(s) 290
BtsIMutI CAGTG 3 cut(s) 123, 134, 313
Cac8I GCNNGC 1 cut(s) 231
Cfr13I GGNCC 2 cut(s) 40, 109
Csp6I GTAC 2 cut(s) 156, 247
CviAII CATG 1 cut(s) 223
CviJI RGCY 7 cut(s) 104, 120, 161, 181, 201, 290, 315
CviKI_1 RGCY 7 cut(s) 104, 120, 161, 181, 201, 290, 315
CviQI GTAC 2 cut(s) 156, 247
DdeI CTNAG 3 cut(s) 51, 126, 255
DpnI GATC 1 cut(s) 212
DpnII GATC 1 cut(s) 210
DraI TTTAAA 1 cut(s) 174
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 2 cut(s) 201, 237
EarI CTCTTC 2 cut(s) 201, 237
Eco24I GRGCYC 1 cut(s) 106
Eco47I GGWCC 2 cut(s) 40, 109
Eco88I CYCGRG 1 cut(s) 105
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 98
EcoT38I GRGCYC 1 cut(s) 106
FaeI CATG 1 cut(s) 226
FaiI YATR 3 cut(s) 38, 224, 296
FaqI GGGAC 1 cut(s) 122
FatI CATG 1 cut(s) 222
FriOI GRGCYC 1 cut(s) 106
FspBI CTAG 1 cut(s) 198
GsuI CTGGAG 1 cut(s) 158
HaeIII GGCC 1 cut(s) 290
Hin1II CATG 1 cut(s) 226
HinfI GANTC 3 cut(s) 58, 65, 257
Hpy166II GTNNAC 1 cut(s) 249
Hpy188III TCNNGA 2 cut(s) 62, 107
Hpy8I GTNNAC 1 cut(s) 249
HpyAV CCTTC 1 cut(s) 76
HpyCH4V TGCA 2 cut(s) 222, 271
HpyF3I CTNAG 3 cut(s) 51, 126, 255
Hsp92II CATG 1 cut(s) 226
Kzo9I GATC 1 cut(s) 210
LpnPI CCDG 7 cut(s) 47, 85, 102, 112, 122, 135, 199
LweI GCATC 1 cut(s) 120
MaeI CTAG 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 31
MalI GATC 1 cut(s) 212
MboI GATC 1 cut(s) 210
MboII GAAGA 3 cut(s) 29, 218, 254
MhlI GDGCHC 1 cut(s) 106
MlsI TGGCCA 1 cut(s) 290
MluCI AATT 2 cut(s) 21, 25
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 1 cut(s) 266
MnlI CCTC 3 cut(s) 48, 133, 309
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
MseI TTAA 4 cut(s) 24, 75, 173, 237
MslI CAYNNNNRTG 1 cut(s) 128
Msp20I TGGCCA 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 100
MvaI CCWGG 1 cut(s) 100
NdeII GATC 1 cut(s) 210
NlaIII CATG 1 cut(s) 226
NlaIV GGNNCC 2 cut(s) 42, 110
PfeI GAWTC 2 cut(s) 58, 65
PflMI CCANNNNNTGG 1 cut(s) 308
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 109
PshBI ATTAAT 1 cut(s) 24
Psp5II RGGWCCY 1 cut(s) 109
Psp6I CCWGG 1 cut(s) 98
PspGI CCWGG 1 cut(s) 98
PspN4I GGNNCC 2 cut(s) 42, 110
PspPI GGNCC 2 cut(s) 40, 109
PspPPI RGGWCCY 1 cut(s) 109
RsaI GTAC 2 cut(s) 157, 248
RsaNI GTAC 2 cut(s) 156, 247
RseI CAYNNNNRTG 1 cut(s) 128
SaqAI TTAA 4 cut(s) 24, 75, 173, 237
Sau3AI GATC 1 cut(s) 210
Sau96I GGNCC 2 cut(s) 40, 109
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 1 cut(s) 100
SduI GDGCHC 1 cut(s) 106
SetI ASST 6 cut(s) 87, 114, 144, 183, 203, 301
SfaNI GCATC 1 cut(s) 120
SinI GGWCC 2 cut(s) 40, 109
SmiMI CAYNNNNRTG 1 cut(s) 128
Sse9I AATT 2 cut(s) 21, 25
SspMI CTAG 1 cut(s) 198
StyD4I CCNGG 1 cut(s) 98
TasI AATT 2 cut(s) 21, 25
TatI WGTACW 2 cut(s) 155, 246
TfiI GAWTC 2 cut(s) 58, 65
Tru1I TTAA 4 cut(s) 24, 75, 173, 237
Tru9I TTAA 4 cut(s) 24, 75, 173, 237
TscAI CASTG 3 cut(s) 130, 141, 313
TspDTI ATGAA 2 cut(s) 95, 255
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 3 cut(s) 130, 141, 313
Van91I CCANNNNNTGG 1 cut(s) 308
VpaK11BI GGWCC 2 cut(s) 40, 109
VspI ATTAAT 1 cut(s) 24
XspI CTAG 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.