MD10G1004200.v1.1

Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014109
Physical Location & Seq
Forward (+)
720010 .. 722977
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1004200.v1.1.491

Sequence Viewer

Length: 318 bp
ATGGATTCCAAACAAGAAGAGATGAAGACGGCAGCCACAGACTCTACAAGTAAGGGGATCGAGGGTTCATCTGTTAAGATAAAGGTGCTGTTCTTTGCGAGGGCTCGTGATCTAACTGGCTTGAGTGATACGCAACTGGAGGTGTCGTCTGGTAGTACTGCACTTGATTGTTTAGATAAGCTCATCACCATGTTTCCAAGCTTGACAGAGATCCGCGGGTGCATGGTGCTTGCTCTTAATGAGGCGTACACAGCTGATTCTGCGGTCGTTAAAGACAAGGATGAGTTGGCCATAATACCTCCAATAAGTGGTGGATAA

Protein Analysis

106

Amino Acids

11.15

Weight (kDa)

4.59

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiS PF02597 29 - 105 2.5e-14 ThiS family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014109)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G10100 AT4G10100 AT4G10100
fragaria_vesca FvH4_2g00750
malus_domestica MD05G1003200.v1.1 MD10G1004200.v1.1
prunus_persica Prupe.8G006600_v2.0.a1
pyrus_communis pycom05g00290
rosa_chinensis RchiOBHm_Chr6g0251131
rosa_laevigata RLG00000015481
rosa_roxburghii Rroxscaffold_7G00217010
rosa_rugosa Rorug05G0500600
rosa_samantha Rh6AG009500 Rh6BG002400 Rh6CG006300 Rh6DG006600
rosa_wichuraiana Rw6G000950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 308
AccII CGCG 1 cut(s) 216
AciI CCGC 3 cut(s) 214, 216, 263
AclWI GGATC 2 cut(s) 65, 205
AcoI YGGCCR 1 cut(s) 288
AfaI GTAC 2 cut(s) 157, 248
AfiI CCNNNNNNNGG 1 cut(s) 308
AloI GAACNNNNNNTCC 2 cut(s) 49, 81
AluBI AGCT 3 cut(s) 181, 201, 254
AluI AGCT 3 cut(s) 181, 201, 254
AlwI GGATC 2 cut(s) 65, 205
AoxI GGCC 1 cut(s) 288
ApeKI GCWGC 1 cut(s) 32
AsuHPI GGTGA 1 cut(s) 178
BalI TGGCCA 1 cut(s) 290
BanII GRGCYC 1 cut(s) 106
BauI CACGAG 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 32
BbvI GCAGC 1 cut(s) 44
BceAI ACGGC 1 cut(s) 45
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
BmcAI AGTACT 1 cut(s) 157
BpiI GAAGAC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 142
BsaJI CCNNGG 1 cut(s) 214
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse1I ACTGG 2 cut(s) 121, 141
BseDI CCNNGG 1 cut(s) 214
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 121, 141
BseXI GCAGC 1 cut(s) 44
BsgI GTGCAG 1 cut(s) 144
Bsh1236I CGCG 1 cut(s) 216
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 1 cut(s) 290
BsiEI CGRYCG 1 cut(s) 267
BslI CCNNNNNNNGG 1 cut(s) 308
BsnI GGCC 1 cut(s) 290
Bsp1286I GDGCHC 1 cut(s) 106
Bsp143I GATC 3 cut(s) 57, 109, 210
BspACI CCGC 3 cut(s) 214, 216, 263
BspANI GGCC 1 cut(s) 290
BspFNI CGCG 1 cut(s) 216
BspPI GGATC 2 cut(s) 65, 205
BsrI ACTGG 2 cut(s) 121, 141
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 3 cut(s) 57, 109, 210
BssSI CACGAG 1 cut(s) 105
Bst2BI CACGAG 1 cut(s) 105
Bst6I CTCTTC 1 cut(s) 12
BstC8I GCNNGC 1 cut(s) 231
BstDSI CCRYGG 1 cut(s) 214
BstF5I GGATG 1 cut(s) 286
BstFNI CGCG 1 cut(s) 216
BstKTI GATC 3 cut(s) 60, 112, 213
BstMBI GATC 3 cut(s) 57, 109, 210
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 2 cut(s) 251, 260
BstUI CGCG 1 cut(s) 216
BstV1I GCAGC 1 cut(s) 44
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 1 cut(s) 210
BstYI RGATCY 1 cut(s) 210
BsuRI GGCC 1 cut(s) 290
BtgI CCRYGG 1 cut(s) 214
BtsCI GGATG 1 cut(s) 286
Cac8I GCNNGC 1 cut(s) 231
Cfr42I CCGCGG 1 cut(s) 217
Csp6I GTAC 2 cut(s) 156, 247
CviAII CATG 2 cut(s) 190, 223
CviJI RGCY 7 cut(s) 35, 104, 120, 181, 201, 254, 290
CviKI_1 RGCY 7 cut(s) 35, 104, 120, 181, 201, 254, 290
CviQI GTAC 2 cut(s) 156, 247
DpnI GATC 3 cut(s) 59, 111, 212
DpnII GATC 3 cut(s) 57, 109, 210
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco24I GRGCYC 1 cut(s) 106
EcoT38I GRGCYC 1 cut(s) 106
FaeI CATG 2 cut(s) 193, 226
FaiI YATR 3 cut(s) 191, 224, 293
FatI CATG 2 cut(s) 189, 222
FauI CCCGC 1 cut(s) 209
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 1 cut(s) 106
Fsp4HI GCNGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 33
GsuI CTGGAG 1 cut(s) 158
HaeIII GGCC 1 cut(s) 290
Hin1II CATG 2 cut(s) 193, 226
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 3 cut(s) 5, 41, 257
HphI GGTGA 1 cut(s) 178
Hpy166II GTNNAC 1 cut(s) 249
Hpy188III TCNNGA 1 cut(s) 107
Hpy8I GTNNAC 1 cut(s) 249
HpyCH4V TGCA 2 cut(s) 161, 222
HpyF10VI GCNNNNNNNGC 2 cut(s) 251, 260
Hsp92II CATG 2 cut(s) 193, 226
KspI CCGCGG 1 cut(s) 217
Kzo9I GATC 3 cut(s) 57, 109, 210
LpnPI CCDG 3 cut(s) 102, 122, 135
Lsp1109I GCAGC 1 cut(s) 44
MalI GATC 3 cut(s) 59, 111, 212
MboI GATC 3 cut(s) 57, 109, 210
MboII GAAGA 2 cut(s) 29, 37
MflI RGATCY 1 cut(s) 210
MhlI GDGCHC 1 cut(s) 106
MlsI TGGCCA 1 cut(s) 290
MluNI TGGCCA 1 cut(s) 290
MlyI GAGTC 1 cut(s) 35
MnlI CCTC 5 cut(s) 55, 93, 133, 235, 309
Mox20I TGGCCA 1 cut(s) 290
MscI TGGCCA 1 cut(s) 290
MseI TTAA 3 cut(s) 75, 237, 270
MslI CAYNNNNRTG 1 cut(s) 188
Msp20I TGGCCA 1 cut(s) 290
MspA1I CMGCKG 2 cut(s) 216, 254
MvnI CGCG 1 cut(s) 216
MwoI GCNNNNNNNGC 2 cut(s) 251, 260
NdeII GATC 3 cut(s) 57, 109, 210
NlaIII CATG 2 cut(s) 193, 226
PfeI GAWTC 2 cut(s) 5, 257
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 1 cut(s) 35
PpsI GAGTC 1 cut(s) 35
PsuI RGATCY 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 254
RsaI GTAC 2 cut(s) 157, 248
RsaNI GTAC 2 cut(s) 156, 247
RseI CAYNNNNRTG 1 cut(s) 188
SacII CCGCGG 1 cut(s) 217
SaqAI TTAA 3 cut(s) 75, 237, 270
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 3 cut(s) 57, 109, 210
ScaI AGTACT 1 cut(s) 157
SchI GAGTC 1 cut(s) 35
SduI GDGCHC 1 cut(s) 106
SetI ASST 6 cut(s) 87, 144, 183, 203, 256, 301
Sfr303I CCGCGG 1 cut(s) 217
SgrBI CCGCGG 1 cut(s) 217
SmiMI CAYNNNNRTG 1 cut(s) 188
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
SsiI CCGC 3 cut(s) 214, 216, 263
TaqI TCGA 1 cut(s) 60
TatI WGTACW 1 cut(s) 155
TfiI GAWTC 2 cut(s) 5, 257
Tru1I TTAA 3 cut(s) 75, 237, 270
Tru9I TTAA 3 cut(s) 75, 237, 270
TseI GCWGC 1 cut(s) 32
TspDTI ATGAA 2 cut(s) 38, 57
Van91I CCANNNNNTGG 1 cut(s) 308
ZrmI AGTACT 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.