AT4G16400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016551
Physical Location & Seq
Forward (+)
9260564 .. 9262670
2107 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G16400.1

Sequence Viewer

Length: 657 bp
ATGGGAAACTGCATTCACACGTTGCTCTGCAAGCATGGAAACAAGGTTACAGATGTCTGGTCGGATGATGAGGCATCAACAGAGAAGATCAAGGAGTCACCTCAGGATGCAGCTGAGCAGAAGAAAACAGGGACAAGGCGGTGCATAAAGATCACTCTGACTAGGAAACAGCTCGAGCAGCTGCTGCAACAAAATCCTCAAGGCGTTTCATTTCAGCGGAAATGGAAGCCATCTTTGCAGACAATAGTAGAGAAGATCTTGTGGCTGCTGATATGTAAAGTTACAGCTCTTGTAATGGAGTTTTTGGATTCAACATCTCTCCATTTCTCTCCTCTCTCTCTTACCAAGTTCAATCACAAGGAAATGGAGATCCAGGCAAAGCTCATGGAATACAGATTCCATTTCATGGTGACAATCATGGTGAGCGTGATTGTGGCGTCTCTCGTGTATGCAGCACCAAGGATTCTGAACATTTTAGCCTATTTCTGGCCTCTGTTTGCGTCAACAGCCGCCTTCTTAGCGATGGCAATCACCTTTGGTGGCTTTCAACAGCTTTCAGAAGAAGCCACCGGCGAGAGACTGATGGATTACGTCGCCGGACGGCCTGAGGATTCTCACAAAAACAACTTGAGAGACGATATAGAAGCTACCACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

218

Amino Acids

24.86

Weight (kDa)

7.7

Isoelectric Point (pI)

42.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16400
fragaria_vesca FvH4_3g00490
malus_domestica MD00G1056900.v1.1
prunus_persica Prupe.4G005500_v2.0.a1
pyrus_communis pycom05g32680
rosa_chinensis RchiOBHm_Chr5g0000661
rosa_multiflora Rmu_sc0011992.1_g000011
rosa_roxburghii Rroxscaffold_1G00075540
rosa_rugosa Rorug04G0385600
rosa_samantha Rh5AG005700 Rh5BG007300 Rh5CG006100 Rh5DG005900
rosa_wichuraiana Rw5G000560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 406
AciI CCGC 3 cut(s) 139, 217, 510
AclWI GGATC 1 cut(s) 364
AcyI GRCGYC 1 cut(s) 437
AfiI CCNNNNNNNGG 2 cut(s) 406, 486
AflIII ACRYGT 1 cut(s) 18
AgsI TTSAA 3 cut(s) 312, 352, 548
AjnI CCWGG 1 cut(s) 372
AluBI AGCT 7 cut(s) 113, 172, 181, 287, 382, 553, 647
AluI AGCT 7 cut(s) 113, 172, 181, 287, 382, 553, 647
Alw26I GTCTC 3 cut(s) 444, 571, 627
AlwI GGATC 1 cut(s) 364
AlwNI CAGNNNCTG 1 cut(s) 184
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 2 cut(s) 488, 602
ApeKI GCWGC 6 cut(s) 110, 178, 181, 184, 265, 452
AsuHPI GGTGA 4 cut(s) 90, 421, 433, 523
AvaI CYCGRG 1 cut(s) 173
AxyI CCTNAGG 2 cut(s) 102, 606
BauI CACGAG 1 cut(s) 443
BbvI GCAGC 6 cut(s) 122, 168, 171, 190, 252, 464
BccI CCATC 3 cut(s) 238, 517, 577
BceAI ACGGC 1 cut(s) 617
BciT130I CCWGG 1 cut(s) 374
BcoDI GTCTC 3 cut(s) 444, 571, 627
BfaI CTAG 1 cut(s) 162
BglII AGATCT 1 cut(s) 255
BisI GCNGC 7 cut(s) 111, 179, 182, 185, 266, 453, 510
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 7 cut(s) 112, 180, 183, 186, 267, 454, 511
Bme1390I CCNGG 1 cut(s) 374
BmeT110I CYCGRG 1 cut(s) 173
BmrFI CCNGG 1 cut(s) 374
BmsI GCATC 2 cut(s) 83, 97
Bpu1102I GCTNAGC 1 cut(s) 114
BpuEI CTTGAG 2 cut(s) 183, 649
BsaBI GATNNNNATC 1 cut(s) 527
BsaHI GRCGYC 1 cut(s) 437
BsaJI CCNNGG 1 cut(s) 458
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 486
Bse118I RCCGGY 1 cut(s) 569
Bse21I CCTNAGG 2 cut(s) 102, 606
Bse8I GATNNNNATC 1 cut(s) 527
BseBI CCWGG 1 cut(s) 374
BseDI CCNNGG 1 cut(s) 458
BseGI GGATG 2 cut(s) 70, 112
BseJI GATNNNNATC 1 cut(s) 527
BseLI CCNNNNNNNGG 2 cut(s) 406, 486
BseMII CTCAG 3 cut(s) 105, 116, 597
BseRI GAGGAG 1 cut(s) 321
BseXI GCAGC 6 cut(s) 122, 168, 171, 190, 252, 464
BshFI GGCC 2 cut(s) 490, 604
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 2 cut(s) 570, 597
BslFI GGGAC 1 cut(s) 145
BslI CCNNNNNNNGG 2 cut(s) 406, 486
BsmAI GTCTC 3 cut(s) 444, 571, 627
BsmBI CGTCTC 2 cut(s) 444, 627
BsmFI GGGAC 1 cut(s) 145
BsmI GAATGC 1 cut(s) 12
BsnI GGCC 2 cut(s) 490, 604
BsoBI CYCGRG 1 cut(s) 173
Bsp143I GATC 4 cut(s) 87, 150, 255, 369
Bsp1720I GCTNAGC 1 cut(s) 114
BspACI CCGC 3 cut(s) 139, 217, 510
BspANI GGCC 2 cut(s) 490, 604
BspCNI CTCAG 3 cut(s) 106, 115, 598
BspPI GGATC 1 cut(s) 364
BsrFI RCCGGY 1 cut(s) 569
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 1 cut(s) 458
BssMI GATC 4 cut(s) 87, 150, 255, 369
BssNI GRCGYC 1 cut(s) 437
BssSI CACGAG 1 cut(s) 443
BssT1I CCWWGG 1 cut(s) 458
Bst2BI CACGAG 1 cut(s) 443
Bst2UI CCWGG 1 cut(s) 374
BstACI GRCGYC 1 cut(s) 437
BstAPI GCANNNNNTGC 1 cut(s) 184
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 4 cut(s) 102, 114, 517, 606
BstF5I GGATG 2 cut(s) 70, 112
BstKTI GATC 4 cut(s) 90, 153, 258, 372
BstMAI GTCTC 3 cut(s) 444, 571, 627
BstMBI GATC 4 cut(s) 87, 150, 255, 369
BstMWI GCNNNNNNNGC 6 cut(s) 31, 178, 184, 235, 506, 518
BstNI CCWGG 1 cut(s) 374
BstSCI CCNGG 1 cut(s) 372
BstV1I GCAGC 6 cut(s) 122, 168, 171, 190, 252, 464
BstX2I RGATCY 2 cut(s) 255, 369
BstYI RGATCY 2 cut(s) 255, 369
Bsu36I CCTNAGG 2 cut(s) 102, 606
BsuRI GGCC 2 cut(s) 490, 604
BtgZI GCGATG 1 cut(s) 536
BtsCI GGATG 2 cut(s) 70, 112
Cac8I GCNNGC 1 cut(s) 32
CaiI CAGNNNCTG 1 cut(s) 184
Cfr10I RCCGGY 1 cut(s) 569
CseI GACGC 2 cut(s) 426, 489
CviAII CATG 4 cut(s) 35, 385, 406, 418
DdeI CTNAG 4 cut(s) 102, 114, 517, 606
DpnI GATC 4 cut(s) 89, 152, 257, 371
DpnII GATC 4 cut(s) 87, 150, 255, 369
Eco130I CCWWGG 1 cut(s) 458
Eco81I CCTNAGG 2 cut(s) 102, 606
Eco88I CYCGRG 1 cut(s) 173
EcoRII CCWGG 1 cut(s) 372
EcoT14I CCWWGG 1 cut(s) 458
ErhI CCWWGG 1 cut(s) 458
Esp3I CGTCTC 2 cut(s) 444, 627
FaeI CATG 4 cut(s) 38, 388, 409, 421
FaiI YATR 8 cut(s) 36, 146, 274, 386, 407, 419, 450, 641
FaqI GGGAC 1 cut(s) 145
FatI CATG 4 cut(s) 34, 384, 405, 417
Fnu4HI GCNGC 7 cut(s) 111, 179, 182, 185, 266, 453, 510
FokI GGATG 2 cut(s) 77, 119
Fsp4HI GCNGC 7 cut(s) 111, 179, 182, 185, 266, 453, 510
FspBI CTAG 1 cut(s) 162
GluI GCNGC 7 cut(s) 111, 179, 182, 185, 266, 453, 510
HaeIII GGCC 2 cut(s) 490, 604
HapII CCGG 2 cut(s) 570, 597
HgaI GACGC 2 cut(s) 426, 489
Hin1I GRCGYC 1 cut(s) 437
Hin1II CATG 4 cut(s) 38, 388, 409, 421
HincII GTYRAC 1 cut(s) 504
HindII GTYRAC 1 cut(s) 504
HinfI GANTC 5 cut(s) 95, 308, 396, 463, 611
HpaII CCGG 2 cut(s) 570, 597
HphI GGTGA 4 cut(s) 90, 421, 433, 523
Hpy166II GTNNAC 1 cut(s) 504
Hpy188I TCNGA 4 cut(s) 64, 159, 468, 559
Hpy188III TCNNGA 1 cut(s) 104
Hpy8I GTNNAC 1 cut(s) 504
Hpy99I CGWCG 1 cut(s) 596
HpyAV CCTTC 1 cut(s) 523
HpyCH4IV ACGT 2 cut(s) 20, 591
HpyCH4V TGCA 7 cut(s) 12, 30, 110, 144, 187, 238, 452
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 178, 184, 235, 506, 518
HpyF3I CTNAG 4 cut(s) 102, 114, 517, 606
HpySE526I ACGT 2 cut(s) 20, 591
Hsp92I GRCGYC 1 cut(s) 437
Hsp92II CATG 4 cut(s) 38, 388, 409, 421
Kzo9I GATC 4 cut(s) 87, 150, 255, 369
LpnPI CCDG 9 cut(s) 43, 89, 114, 359, 386, 472, 583, 610, 618
Lsp1109I GCAGC 6 cut(s) 122, 168, 171, 190, 252, 464
LweI GCATC 2 cut(s) 83, 97
MaeI CTAG 1 cut(s) 162
MaeII ACGT 2 cut(s) 20, 591
MaeIII GTNAC 4 cut(s) 46, 96, 280, 409
MalI GATC 4 cut(s) 89, 152, 257, 371
MboI GATC 4 cut(s) 87, 150, 255, 369
MboII GAAGA 4 cut(s) 97, 133, 265, 572
MflI RGATCY 2 cut(s) 255, 369
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 1 cut(s) 42
MnlI CCTC 6 cut(s) 64, 111, 207, 342, 501, 601
MspA1I CMGCKG 3 cut(s) 113, 181, 217
MspI CCGG 2 cut(s) 570, 597
MspR9I CCNGG 1 cut(s) 374
Mva1269I GAATGC 1 cut(s) 12
MvaI CCWGG 1 cut(s) 374
MwoI GCNNNNNNNGC 6 cut(s) 31, 178, 184, 235, 506, 518
NdeII GATC 4 cut(s) 87, 150, 255, 369
NlaIII CATG 4 cut(s) 38, 388, 409, 421
NmuCI GTSAC 2 cut(s) 96, 409
PaeR7I CTCGAG 1 cut(s) 173
PctI GAATGC 1 cut(s) 12
PfeI GAWTC 4 cut(s) 308, 396, 463, 611
PflMI CCANNNNNTGG 1 cut(s) 406
PkrI GCNGC 7 cut(s) 112, 180, 183, 186, 267, 454, 511
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
Psp6I CCWGG 1 cut(s) 372
PspGI CCWGG 1 cut(s) 372
PspXI VCTCGAGB 1 cut(s) 173
PstNI CAGNNNCTG 1 cut(s) 184
PsuI RGATCY 2 cut(s) 255, 369
PvuII CAGCTG 2 cut(s) 113, 181
SatI GCNGC 7 cut(s) 111, 179, 182, 185, 266, 453, 510
Sau3AI GATC 4 cut(s) 87, 150, 255, 369
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 1 cut(s) 374
SfaNI GCATC 2 cut(s) 83, 97
Sfr274I CTCGAG 1 cut(s) 173
SgrAI CRCCGGYG 1 cut(s) 569
SlaI CTCGAG 1 cut(s) 173
SmlI CTYRAG 3 cut(s) 173, 198, 628
SmoI CTYRAG 3 cut(s) 173, 198, 628
SsiI CCGC 3 cut(s) 139, 217, 510
SspMI CTAG 1 cut(s) 162
StyD4I CCNGG 1 cut(s) 372
StyI CCWWGG 1 cut(s) 458
TaiI ACGT 2 cut(s) 23, 594
TaqI TCGA 1 cut(s) 174
TauI GCSGC 1 cut(s) 512
TfiI GAWTC 4 cut(s) 308, 396, 463, 611
TseFI GTSAC 2 cut(s) 96, 409
TseI GCWGC 6 cut(s) 110, 178, 181, 184, 265, 452
Tsp45I GTSAC 2 cut(s) 96, 409
TspDTI ATGAA 2 cut(s) 198, 394
Van91I CCANNNNNTGG 1 cut(s) 406
XhoI CTCGAG 1 cut(s) 173
XspI CTAG 1 cut(s) 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.