AT4G16640

Belongs to the peptidase M10A family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0015037
Physical Location & Seq
Reverse (-)
9367147 .. 9368852
1706 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G16640.1

Sequence Viewer

Length: 1095 bp
ATGTCTCGTAATTTAATCTATAGAAGAAACAGAGCTCTCTGTTTTGTTTTAATCTTGTTCTGCTTCCCTTACCGGTTTGGCGCTAGAAACACGCCGGAGGCGGAACAATCCACAGCAAAAGCAACTCAAATAATACACGTCAGCAATTCCACGTGGCATGATTTCTCTCGTCTTGTAGATGTCCAAATAGGTAGCCACGTCAGCGGCGTATCAGAGCTCAAAAGATACCTCCACCGATTCGGTTACGTCAATGATGGCTCCGAAATATTTTCCGACGTGTTCGATGGTCCTCTGGAATCGGCAATCTCTCTGTATCAAGAAAATCTCGGTTTACCAATAACCGGAAGACTCGACACGAGTACAGTTACTCTCATGTCGTTACCGCGATGTGGCGTTAGCGATACGCACATGACCATCAACAACGATTTCCTCCACACAACGGCGCATTATACGTATTTCAACGGTAAACCGAAATGGAACCGTGATACGCTAACCTACGCTATCTCCAAAACTCACAAACTCGATTACTTGACGTCAGAAGACGTCAAAACCGTTTTCCGGCGAGCTTTTTCACAGTGGTCAAGCGTGATTCCGGTGAGTTTCGAGGAAGTCGACGATTTCACGACGGCTGATTTAAAGATCGGATTCTACGCTGGTGATCACGGTGACGGGCTTCCGTTTGACGGTGTACTTGGAACTTTAGCACACGCTTTTGCGCCGGAGAACGGGAGGCTTCACCTCGACGCGGCGGAGACGTGGATCGTCGACGATGACTTGAAAGGATCTTCAGAGGTGGCCGTTGACTTGGAGTCTGTGGCGACTCACGAGATCGGTCACTTGTTGGGATTAGGACATAGCTCGCAGGAGTCGGCGGTTATGTATCCGAGTCTCCGACCGAGGACCAAGAAAGTTGACCTTACGGTTGATGACGTGGCAGGTGTACTTAAGCTATATGGTCCGAATCCTAAACTACGGTTGGATTCACTAACGCAGTCGGAAGATTCTATTAAAAACGGCACCGTATCACATAGATTCTTGTCGGGGAATTTTATCGGTTATGTTCTGTTGGTTGTTGGTTTGATTCTTTTCCTATAG

Protein Analysis

364

Amino Acids

40.53

Weight (kDa)

5.81

Isoelectric Point (pI)

32.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 68 - 125 1.2e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 158 - 319 7.8e-53 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16640
fragaria_vesca FvH4_5g19070
malus_domestica MD04G1063000.v1.1 MD06G1057300.v1.1
prunus_persica Prupe.5G068200_v2.0.a1
pyrus_communis pycom04g05670
rosa_chinensis RchiOBHm_Chr7g0203521
rosa_laevigata RLG00000003523
rosa_multiflora Rmu_sc0022597.1_g000010
rosa_roxburghii Rroxscaffold_3G00253740
rosa_rugosa Rorug07G0076400 Rorug07G0076500
rosa_samantha Rh7AG205900 Rh7CG218700 Rh7DG213100
rosa_wichuraiana Rw7G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 926
AatII GACGTC 2 cut(s) 536, 546
Acc36I ACCTGC 1 cut(s) 926
AccB1I GGYRCC 1 cut(s) 1016
AccI GTMKAC 2 cut(s) 612, 765
AccII CGCG 2 cut(s) 385, 746
AciI CCGC 6 cut(s) 101, 204, 383, 746, 749, 872
AclWI GGATC 2 cut(s) 767, 790
AcoI YGGCCR 1 cut(s) 795
AcsI RAATTY 1 cut(s) 1045
AcuI CTGAAG 1 cut(s) 771
AcvI CACGTG 1 cut(s) 153
AcyI GRCGYC 2 cut(s) 533, 543
AfaI GTAC 3 cut(s) 361, 690, 942
AfiI CCNNNNNNNGG 7 cut(s) 341, 389, 439, 558, 683, 725, 745
AflII CTTAAG 1 cut(s) 944
AflIII ACRYGT 2 cut(s) 136, 276
AgeI ACCGGT 1 cut(s) 72
AgsI TTSAA 2 cut(s) 460, 778
AhdI GACNNNNNGTC 1 cut(s) 808
AjiI CACGTC 5 cut(s) 139, 199, 277, 756, 931
AluBI AGCT 5 cut(s) 35, 217, 566, 858, 949
AluI AGCT 5 cut(s) 35, 217, 566, 858, 949
Alw21I GWGCWC 2 cut(s) 37, 219
Alw26I GTCTC 3 cut(s) 9, 746, 893
AlwI GGATC 2 cut(s) 767, 790
AoxI GGCC 1 cut(s) 795
ApoI RAATTY 1 cut(s) 1045
AsiGI ACCGGT 1 cut(s) 72
AspLEI GCGC 3 cut(s) 83, 445, 718
AspS9I GGNCC 3 cut(s) 287, 900, 956
AsuHPI GGTGA 4 cut(s) 607, 668, 677, 728
AvaII GGWCC 3 cut(s) 287, 900, 956
BanI GGYRCC 1 cut(s) 1016
BanII GRGCYC 2 cut(s) 37, 219
BauI CACGAG 2 cut(s) 355, 824
BbrPI CACGTG 1 cut(s) 153
BbsI GAAGAC 2 cut(s) 352, 546
Bbv12I GWGCWC 2 cut(s) 37, 219
BccI CCATC 3 cut(s) 248, 278, 422
BceAI ACGGC 4 cut(s) 456, 642, 782, 1030
BciVI GTATCC 1 cut(s) 891
BclI TGATCA 1 cut(s) 658
BcoDI GTCTC 3 cut(s) 9, 746, 893
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 2 cut(s) 19, 1091
BfoI RGCGCY 1 cut(s) 84
BfrI CTTAAG 1 cut(s) 944
BfuAI ACCTGC 1 cut(s) 926
BfuI GTATCC 1 cut(s) 891
BisI GCNGC 2 cut(s) 205, 747
BlsI GCNGC 2 cut(s) 206, 748
Bme18I GGWCC 3 cut(s) 287, 900, 956
BmeRI GACNNNNNGTC 1 cut(s) 808
BmgBI CACGTC 5 cut(s) 139, 199, 277, 756, 931
BmgT120I GGNCC 3 cut(s) 287, 900, 956
BmiI GGNNCC 3 cut(s) 259, 479, 1018
BpiI GAAGAC 2 cut(s) 352, 546
BsaAI YACGTR 2 cut(s) 153, 453
BsaHI GRCGYC 2 cut(s) 533, 543
BsaJI CCNNGG 1 cut(s) 896
BsaWI WCCGGW 3 cut(s) 72, 341, 592
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 7 cut(s) 341, 389, 439, 558, 683, 725, 745
Bse118I RCCGGY 1 cut(s) 72
BseDI CCNNGG 1 cut(s) 896
BseLI CCNNNNNNNGG 7 cut(s) 341, 389, 439, 558, 683, 725, 745
Bsh1236I CGCG 2 cut(s) 385, 746
Bsh1285I CGRYCG 1 cut(s) 896
BshFI GGCC 1 cut(s) 797
BshNI GGYRCC 1 cut(s) 1016
BshTI ACCGGT 1 cut(s) 72
BsiEI CGRYCG 1 cut(s) 896
BsiHKAI GWGCWC 2 cut(s) 37, 219
BsiSI CCGG 6 cut(s) 73, 95, 342, 559, 593, 719
BslI CCNNNNNNNGG 7 cut(s) 341, 389, 439, 558, 683, 725, 745
BsmAI GTCTC 3 cut(s) 9, 746, 893
BsmBI CGTCTC 1 cut(s) 746
BsnI GGCC 1 cut(s) 797
Bsp1286I GDGCHC 2 cut(s) 37, 219
Bsp143I GATC 5 cut(s) 639, 658, 759, 782, 828
BspACI CCGC 6 cut(s) 101, 204, 383, 746, 749, 872
BspANI GGCC 1 cut(s) 797
BspFNI CGCG 2 cut(s) 385, 746
BspLI GGNNCC 3 cut(s) 259, 479, 1018
BspMI ACCTGC 1 cut(s) 926
BspPI GGATC 2 cut(s) 767, 790
BspT107I GGYRCC 1 cut(s) 1016
BspTI CTTAAG 1 cut(s) 944
BsrFI RCCGGY 1 cut(s) 72
BssAI RCCGGY 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 896
BssMI GATC 5 cut(s) 639, 658, 759, 782, 828
BssNI GRCGYC 2 cut(s) 533, 543
BssSI CACGAG 2 cut(s) 355, 824
Bst2BI CACGAG 2 cut(s) 355, 824
BstACI GRCGYC 2 cut(s) 533, 543
BstAFI CTTAAG 1 cut(s) 944
BstBAI YACGTR 2 cut(s) 153, 453
BstC8I GCNNGC 2 cut(s) 564, 860
BstFNI CGCG 2 cut(s) 385, 746
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 3 cut(s) 83, 445, 718
BstKTI GATC 5 cut(s) 642, 661, 762, 785, 831
BstMAI GTCTC 3 cut(s) 9, 746, 893
BstMBI GATC 5 cut(s) 639, 658, 759, 782, 828
BstMCI CGRYCG 1 cut(s) 896
BstMWI GCNNNNNNNGC 1 cut(s) 201
BstSFI CTRYAG 2 cut(s) 19, 1091
BstSNI TACGTA 1 cut(s) 453
BstUI CGCG 2 cut(s) 385, 746
BstV2I GAAGAC 2 cut(s) 352, 546
BstX2I RGATCY 1 cut(s) 782
BstYI RGATCY 1 cut(s) 782
BsuI GTATCC 1 cut(s) 891
BsuRI GGCC 1 cut(s) 797
BtgZI GCGATG 1 cut(s) 400
BtrI CACGTC 5 cut(s) 139, 199, 277, 756, 931
BtsIMutI CAGTG 1 cut(s) 581
BveI ACCTGC 1 cut(s) 926
Cac8I GCNNGC 2 cut(s) 564, 860
CfoI GCGC 3 cut(s) 83, 445, 718
Cfr10I RCCGGY 1 cut(s) 72
Cfr13I GGNCC 3 cut(s) 287, 900, 956
CseI GACGC 1 cut(s) 752
Csp6I GTAC 3 cut(s) 360, 689, 941
CspAI ACCGGT 1 cut(s) 72
CviAII CATG 3 cut(s) 158, 373, 409
CviQI GTAC 3 cut(s) 360, 689, 941
DpnI GATC 5 cut(s) 641, 660, 761, 784, 830
DpnII GATC 5 cut(s) 639, 658, 759, 782, 828
DraI TTTAAA 1 cut(s) 636
DriI GACNNNNNGTC 1 cut(s) 808
EaeI YGGCCR 1 cut(s) 795
Eam1105I GACNNNNNGTC 1 cut(s) 808
EciI GGCGGA 2 cut(s) 116, 764
Ecl136II GAGCTC 2 cut(s) 35, 217
Eco105I TACGTA 1 cut(s) 453
Eco24I GRGCYC 2 cut(s) 37, 219
Eco47I GGWCC 3 cut(s) 287, 900, 956
Eco53kI GAGCTC 2 cut(s) 35, 217
Eco57I CTGAAG 1 cut(s) 771
Eco72I CACGTG 1 cut(s) 153
EcoICRI GAGCTC 2 cut(s) 35, 217
EcoT38I GRGCYC 2 cut(s) 37, 219
Esp3I CGTCTC 1 cut(s) 746
FaeI CATG 3 cut(s) 161, 376, 412
FalI AAGNNNNNCTT 2 cut(s) 900, 932
FatI CATG 3 cut(s) 157, 372, 408
FbaI TGATCA 1 cut(s) 658
FblI GTMKAC 2 cut(s) 612, 765
Fnu4HI GCNGC 2 cut(s) 205, 747
FriOI GRGCYC 2 cut(s) 37, 219
Fsp4HI GCNGC 2 cut(s) 205, 747
FspBI CTAG 1 cut(s) 84
GlaI GCGC 3 cut(s) 82, 444, 717
GluI GCNGC 2 cut(s) 205, 747
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 1 cut(s) 797
HapII CCGG 6 cut(s) 73, 95, 342, 559, 593, 719
HgaI GACGC 1 cut(s) 752
HhaI GCGC 3 cut(s) 83, 445, 718
Hin1I GRCGYC 2 cut(s) 533, 543
Hin1II CATG 3 cut(s) 161, 376, 412
Hin6I GCGC 3 cut(s) 81, 443, 716
HinP1I GCGC 3 cut(s) 81, 443, 716
HincII GTYRAC 4 cut(s) 613, 766, 802, 913
HindII GTYRAC 4 cut(s) 613, 766, 802, 913
HpaII CCGG 6 cut(s) 73, 95, 342, 559, 593, 719
HphI GGTGA 4 cut(s) 607, 668, 677, 728
Hpy166II GTNNAC 8 cut(s) 332, 467, 613, 689, 766, 802, 913, 941
Hpy188III TCNNGA 4 cut(s) 293, 317, 622, 824
Hpy8I GTNNAC 8 cut(s) 332, 467, 613, 689, 766, 802, 913, 941
Hpy99I CGWCG 6 cut(s) 278, 617, 628, 746, 767, 770
HpyF10VI GCNNNNNNNGC 1 cut(s) 201
Hsp92I GRCGYC 2 cut(s) 533, 543
Hsp92II CATG 3 cut(s) 161, 376, 412
HspAI GCGC 3 cut(s) 81, 443, 716
Ksp22I TGATCA 1 cut(s) 658
Kzo9I GATC 5 cut(s) 639, 658, 759, 782, 828
LmnI GCTCC 1 cut(s) 263
MaeI CTAG 1 cut(s) 84
MaeIII GTNAC 5 cut(s) 242, 364, 378, 665, 833
MalI GATC 5 cut(s) 641, 660, 761, 784, 830
MboI GATC 5 cut(s) 639, 658, 759, 782, 828
MboII GAAGA 5 cut(s) 36, 357, 551, 777, 1010
MflI RGATCY 1 cut(s) 782
MhlI GDGCHC 2 cut(s) 37, 219
MluCI AATT 3 cut(s) 10, 145, 1045
MlyI GAGTC 5 cut(s) 342, 814, 818, 875, 895
MmeI TCCRAC 4 cut(s) 297, 916, 957, 975
MnlI CCTC 9 cut(s) 91, 239, 300, 440, 598, 723, 749, 784, 891
MseI TTAA 5 cut(s) 14, 50, 635, 945, 1008
MspA1I CMGCKG 1 cut(s) 204
MspCI CTTAAG 1 cut(s) 944
MspI CCGG 6 cut(s) 73, 95, 342, 559, 593, 719
MvnI CGCG 2 cut(s) 385, 746
MwoI GCNNNNNNNGC 1 cut(s) 201
NdeII GATC 5 cut(s) 639, 658, 759, 782, 828
NlaIII CATG 3 cut(s) 161, 376, 412
NlaIV GGNNCC 3 cut(s) 259, 479, 1018
NmuCI GTSAC 2 cut(s) 665, 833
PaqCI CACCTGC 1 cut(s) 926
PcsI WCGNNNNNNNCGW 3 cut(s) 204, 549, 609
PfeI GAWTC 9 cut(s) 237, 296, 589, 645, 961, 980, 1001, 1032, 1081
PinAI ACCGGT 1 cut(s) 72
PkrI GCNGC 2 cut(s) 206, 748
PleI GAGTC 5 cut(s) 342, 814, 817, 874, 894
PmaCI CACGTG 1 cut(s) 153
PmlI CACGTG 1 cut(s) 153
PpsI GAGTC 5 cut(s) 342, 814, 817, 874, 894
Ppu21I YACGTR 2 cut(s) 153, 453
Psp124BI GAGCTC 2 cut(s) 37, 219
PspCI CACGTG 1 cut(s) 153
PspN4I GGNNCC 3 cut(s) 259, 479, 1018
PspPI GGNCC 3 cut(s) 287, 900, 956
PsuI RGATCY 1 cut(s) 782
RsaI GTAC 3 cut(s) 361, 690, 942
RsaNI GTAC 3 cut(s) 360, 689, 941
SacI GAGCTC 2 cut(s) 37, 219
SalI GTCGAC 2 cut(s) 611, 764
SaqAI TTAA 5 cut(s) 14, 50, 635, 945, 1008
SatI GCNGC 2 cut(s) 205, 747
Sau3AI GATC 5 cut(s) 639, 658, 759, 782, 828
Sau96I GGNCC 3 cut(s) 287, 900, 956
SchI GAGTC 5 cut(s) 342, 814, 818, 875, 895
SduI GDGCHC 2 cut(s) 37, 219
SfcI CTRYAG 2 cut(s) 19, 1091
SgrDI CGTCGACG 1 cut(s) 764
SinI GGWCC 3 cut(s) 287, 900, 956
SmlI CTYRAG 1 cut(s) 944
SmoI CTYRAG 1 cut(s) 944
SnaBI TACGTA 1 cut(s) 453
Sse9I AATT 3 cut(s) 10, 145, 1045
SsiI CCGC 6 cut(s) 101, 204, 383, 746, 749, 872
SspI AATATT 1 cut(s) 267
SspMI CTAG 1 cut(s) 84
SstI GAGCTC 2 cut(s) 37, 219
TaqI TCGA 7 cut(s) 282, 351, 522, 603, 612, 741, 765
TaqII GACCGA 2 cut(s) 821, 910
TasI AATT 3 cut(s) 10, 145, 1045
TatI WGTACW 3 cut(s) 359, 688, 940
TauI GCSGC 2 cut(s) 207, 749
TfiI GAWTC 9 cut(s) 237, 296, 589, 645, 961, 980, 1001, 1032, 1081
Tru1I TTAA 5 cut(s) 14, 50, 635, 945, 1008
Tru9I TTAA 5 cut(s) 14, 50, 635, 945, 1008
TscAI CASTG 1 cut(s) 581
TseFI GTSAC 2 cut(s) 665, 833
Tsp45I GTSAC 2 cut(s) 665, 833
TspGWI ACGGA 1 cut(s) 666
TspRI CASTG 1 cut(s) 581
Vha464I CTTAAG 1 cut(s) 944
VpaK11BI GGWCC 3 cut(s) 287, 900, 956
XapI RAATTY 1 cut(s) 1045
XmiI GTMKAC 2 cut(s) 612, 765
XspI CTAG 1 cut(s) 84
ZraI GACGTC 2 cut(s) 534, 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.