MD06G1057300.v1.1

Belongs to the peptidase M10A family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015037
Physical Location & Seq
Forward (+)
8773122 .. 8774219
1098 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1057300.v1.1.491

Sequence Viewer

Length: 1098 bp
ATGTTCCCATTTTTTAGGTACAACTCTGTCTTCTTCTTCTTCATATGTCTTTGCTTCCTCTGTTTTTCCCGCCCTGCCTTTCCCGCCAGAATCTTACCGGACTCAGTACCTGTATTAACTGATGCCTCCGACGTCCACAATGCCACATGGCACGGCTTCTCAAGGTTCCTAGACGCCGGCAGAGGAAGCCTAGTCAGCGGCATGTCGGAGCTCAAGAACTACATGCACCGCTTCGGCTACTTACCCAATTCCAACTTCACCGATTTCTTCGACACCGAATTTGAGTCGGCCCTGACGCTATACCAGTCCAAGCTGAGCCTGCCCGTTACAGGCAAGCTGGACACTGACACAATCTCAGCGATCATTTCGCCTAGATGTGGTGTTAGTGATGCGTCGCAACATACTGCCTTGCATGCGACCCGTCACTTCGCCTACTTCTATGGCAAGCCGAGGTGGGAGCGCCCTTCCCCGCTCACTTACGCTTTCTCCCCCAACCACACGGTCAGTTACCTGAACCCATCGGATGTTCGAGACATTTTTTGCCGCGCTTTTTCGCGGTGGTCGGCGGTTATCCCCGTGAACTTCACCGAGACGCAGAACTACACTTCCGCGGATATTAAAATAGGGTTTTACCGCGGCGACCATGGAGACGGGAAGCCGTTCGATGGGGTGTTAGGTGTGCTGGCGCATGCTTTTTCGCCCGAAAACGGGCAGTTCCACCTTGACGAGGCGGAATCGTGGGCCATTGATTTCAAAACGGATAAGTCAAAGGAGGCGATTGATTTGGAATCGGTGGCGACTCATGAGATAGGTCATGTACTCGGGCTGGCTCACTCCTCGGTCAAGGAGGCCGTGATGTACCCGAGCCTGCGTCCTCGGACCAAGAAGGTGAACCTCAGGGTTGATGACGTGGAAGGTGTCCAAGCTTTGTACGGTTCAAACCCTAATTTCAGGTTTAGCTCATTGCTGAGTGAAAATTCTTACAACCATGCAGTTTCTTTGGACCCGAGGTCTTCCTCTAAGTGGTCAGTTTCTTTAACACTCACCATCACTTTGGTATTAATTTTAGGCCTTTGGCAACCTGAGAATTATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

366

Amino Acids

41.14

Weight (kDa)

6.26

Isoelectric Point (pI)

39.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 68 - 121 6.7e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 151 - 312 3.7e-53 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16640
fragaria_vesca FvH4_5g19070
malus_domestica MD04G1063000.v1.1 MD06G1057300.v1.1
prunus_persica Prupe.5G068200_v2.0.a1
pyrus_communis pycom04g05670
rosa_chinensis RchiOBHm_Chr7g0203521
rosa_laevigata RLG00000003523
rosa_multiflora Rmu_sc0022597.1_g000010
rosa_roxburghii Rroxscaffold_3G00253740
rosa_rugosa Rorug07G0076400 Rorug07G0076500
rosa_samantha Rh7AG205900 Rh7CG218700 Rh7DG213100
rosa_wichuraiana Rw7G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AccBSI CCGCTC 1 cut(s) 472
AccII CGCG 4 cut(s) 546, 556, 611, 636
AcsI RAATTY 2 cut(s) 278, 976
AcyI GRCGYC 2 cut(s) 132, 174
AfaI GTAC 5 cut(s) 20, 108, 819, 860, 932
AfiI CCNNNNNNNGG 7 cut(s) 329, 377, 665, 707, 708, 727, 1023
AgsI TTSAA 2 cut(s) 754, 939
AhdI GACNNNNNGTC 1 cut(s) 1009
AjiI CACGTC 1 cut(s) 910
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AloI GAACNNNNNNTCC 2 cut(s) 590, 622
AluBI AGCT 5 cut(s) 211, 313, 337, 926, 960
AluI AGCT 5 cut(s) 211, 313, 337, 926, 960
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 3 cut(s) 525, 584, 642
AlwNI CAGNNNCTG 1 cut(s) 110
Ama87I CYCGRG 3 cut(s) 821, 862, 1006
AoxI GGCC 4 cut(s) 288, 741, 849, 1069
ApoI RAATTY 2 cut(s) 278, 976
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AseI ATTAAT 1 cut(s) 1061
Asp700I GAANNNNTTC 1 cut(s) 659
AspLEI GCGC 3 cut(s) 462, 548, 688
AspS9I GGNCC 4 cut(s) 289, 741, 879, 1003
AsuHPI GGTGA 4 cut(s) 250, 577, 901, 1036
AvaI CYCGRG 3 cut(s) 821, 862, 1006
AvaII GGWCC 2 cut(s) 879, 1003
AxyI CCTNAGG 1 cut(s) 896
BanII GRGCYC 1 cut(s) 213
BbsI GAAGAC 2 cut(s) 22, 1005
Bbv12I GWGCWC 1 cut(s) 213
BccI CCATC 3 cut(s) 526, 659, 1055
BceAI ACGGC 3 cut(s) 169, 643, 836
BcoDI GTCTC 3 cut(s) 525, 584, 642
BfaI CTAG 3 cut(s) 170, 191, 372
BfoI RGCGCY 1 cut(s) 463
BisI GCNGC 3 cut(s) 199, 544, 637
BlpI GCTNAGC 1 cut(s) 314
BlsI GCNGC 3 cut(s) 200, 545, 638
Bme18I GGWCC 2 cut(s) 879, 1003
BmeRI GACNNNNNGTC 1 cut(s) 1009
BmeT110I CYCGRG 3 cut(s) 821, 862, 1006
BmgBI CACGTC 1 cut(s) 910
BmgT120I GGNCC 4 cut(s) 289, 741, 879, 1003
BmiI GGNNCC 2 cut(s) 167, 1005
BmsI GCATC 2 cut(s) 112, 379
BpiI GAAGAC 2 cut(s) 22, 1005
Bpu1102I GCTNAGC 1 cut(s) 314
BpuEI CTTGAG 2 cut(s) 145, 197
BsaHI GRCGYC 2 cut(s) 132, 174
BsaJI CCNNGG 7 cut(s) 449, 609, 634, 643, 837, 875, 1007
BsaWI WCCGGW 1 cut(s) 97
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 7 cut(s) 329, 377, 665, 707, 708, 727, 1023
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 304
Bse21I CCTNAGG 1 cut(s) 896
Bse3DI GCAATG 1 cut(s) 962
BseDI CCNNGG 7 cut(s) 449, 609, 634, 643, 837, 875, 1007
BseGI GGATG 1 cut(s) 529
BseLI CCNNNNNNNGG 7 cut(s) 329, 377, 665, 707, 708, 727, 1023
BseMI GCAATG 1 cut(s) 962
BseMII CTCAG 6 cut(s) 117, 305, 369, 910, 959, 1074
BseNI ACTGG 1 cut(s) 304
BseRI GAGGAG 1 cut(s) 826
Bsh1236I CGCG 4 cut(s) 546, 556, 611, 636
BshFI GGCC 4 cut(s) 290, 743, 851, 1071
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 3 cut(s) 821, 862, 1006
BsiSI CCGG 2 cut(s) 98, 177
BslI CCNNNNNNNGG 7 cut(s) 329, 377, 665, 707, 708, 727, 1023
BsmAI GTCTC 3 cut(s) 525, 584, 642
BsmBI CGTCTC 2 cut(s) 584, 642
BsnI GGCC 4 cut(s) 290, 743, 851, 1071
BsoBI CYCGRG 3 cut(s) 821, 862, 1006
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 1 cut(s) 360
Bsp1720I GCTNAGC 1 cut(s) 314
Bsp19I CCATGG 1 cut(s) 643
BspANI GGCC 4 cut(s) 290, 743, 851, 1071
BspCNI CTCAG 6 cut(s) 116, 306, 368, 909, 960, 1075
BspFNI CGCG 4 cut(s) 546, 556, 611, 636
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 2 cut(s) 167, 1005
BsrBI CCGCTC 1 cut(s) 472
BsrDI GCAATG 1 cut(s) 962
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 7 cut(s) 449, 609, 634, 643, 837, 875, 1007
BssMI GATC 1 cut(s) 360
BssNI GRCGYC 2 cut(s) 132, 174
BssT1I CCWWGG 1 cut(s) 643
Bst4CI ACNGT 2 cut(s) 502, 935
BstACI GRCGYC 2 cut(s) 132, 174
BstC8I GCNNGC 9 cut(s) 178, 320, 335, 414, 446, 684, 690, 828, 869
BstDEI CTNAG 7 cut(s) 103, 314, 355, 896, 968, 1020, 1083
BstDSI CCRYGG 3 cut(s) 609, 634, 643
BstENI CCTNNNNNAGG 1 cut(s) 725
BstF5I GGATG 1 cut(s) 529
BstFNI CGCG 4 cut(s) 546, 556, 611, 636
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 3 cut(s) 462, 548, 688
BstKTI GATC 1 cut(s) 363
BstMAI GTCTC 3 cut(s) 525, 584, 642
BstMBI GATC 1 cut(s) 360
BstMWI GCNNNNNNNGC 5 cut(s) 83, 186, 195, 319, 413
BstNSI RCATGY 4 cut(s) 205, 226, 416, 692
BstUI CGCG 4 cut(s) 546, 556, 611, 636
BstV2I GAAGAC 2 cut(s) 22, 1005
BstXI CCANNNNNNTGG 1 cut(s) 1054
Bsu36I CCTNAGG 1 cut(s) 896
BsuRI GGCC 4 cut(s) 290, 743, 851, 1071
BtgI CCRYGG 3 cut(s) 609, 634, 643
BtrI CACGTC 1 cut(s) 910
BtsCI GGATG 1 cut(s) 529
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 9 cut(s) 178, 320, 335, 414, 446, 684, 690, 828, 869
CaiI CAGNNNCTG 1 cut(s) 110
CciI TCATGA 1 cut(s) 802
CfoI GCGC 3 cut(s) 462, 548, 688
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 4 cut(s) 289, 741, 879, 1003
Cfr42I CCGCGG 2 cut(s) 612, 637
CseI GACGC 5 cut(s) 182, 304, 381, 601, 860
Csp6I GTAC 5 cut(s) 19, 107, 818, 859, 931
CviAII CATG 9 cut(s) 147, 202, 223, 413, 644, 689, 803, 815, 989
CviQI GTAC 5 cut(s) 19, 107, 818, 859, 931
DdeI CTNAG 7 cut(s) 103, 314, 355, 896, 968, 1020, 1083
DpnI GATC 1 cut(s) 362
DpnII GATC 1 cut(s) 360
DriI GACNNNNNGTC 1 cut(s) 1009
Eam1105I GACNNNNNGTC 1 cut(s) 1009
EciI GGCGGA 1 cut(s) 746
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 643
Eco147I AGGCCT 1 cut(s) 1071
Eco24I GRGCYC 1 cut(s) 213
Eco47I GGWCC 2 cut(s) 879, 1003
Eco53kI GAGCTC 1 cut(s) 211
Eco81I CCTNAGG 1 cut(s) 896
Eco88I CYCGRG 3 cut(s) 821, 862, 1006
EcoICRI GAGCTC 1 cut(s) 211
EcoNI CCTNNNNNAGG 1 cut(s) 725
EcoT14I CCWWGG 1 cut(s) 643
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 643
Esp3I CGTCTC 2 cut(s) 584, 642
FaeI CATG 9 cut(s) 150, 205, 226, 416, 647, 692, 806, 818, 992
FatI CATG 9 cut(s) 146, 201, 222, 412, 643, 688, 802, 814, 988
FauI CCCGC 3 cut(s) 77, 91, 477
FauNDI CATATG 1 cut(s) 44
Fnu4HI GCNGC 3 cut(s) 199, 544, 637
FokI GGATG 1 cut(s) 536
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 199, 544, 637
FspBI CTAG 3 cut(s) 170, 191, 372
GlaI GCGC 3 cut(s) 461, 547, 687
GluI GCNGC 3 cut(s) 199, 544, 637
HaeII RGCGCY 1 cut(s) 463
HaeIII GGCC 4 cut(s) 290, 743, 851, 1071
HapII CCGG 2 cut(s) 98, 177
HgaI GACGC 5 cut(s) 182, 304, 381, 601, 860
HhaI GCGC 3 cut(s) 462, 548, 688
Hin1I GRCGYC 2 cut(s) 132, 174
Hin1II CATG 9 cut(s) 150, 205, 226, 416, 647, 692, 806, 818, 992
Hin6I GCGC 3 cut(s) 460, 546, 686
HinP1I GCGC 3 cut(s) 460, 546, 686
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 6 cut(s) 90, 101, 284, 734, 788, 799
HpaII CCGG 2 cut(s) 98, 177
HphI GGTGA 4 cut(s) 250, 577, 901, 1036
Hpy166II GTNNAC 3 cut(s) 136, 580, 892
Hpy188I TCNGA 4 cut(s) 130, 208, 523, 879
Hpy188III TCNNGA 3 cut(s) 214, 530, 803
Hpy8I GTNNAC 3 cut(s) 136, 580, 892
Hpy99I CGWCG 2 cut(s) 134, 397
HpyAV CCTTC 3 cut(s) 474, 880, 908
HpyCH4III ACNGT 2 cut(s) 502, 935
HpyCH4IV ACGT 2 cut(s) 132, 909
HpyCH4V TGCA 3 cut(s) 226, 412, 992
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 186, 195, 319, 413
HpyF3I CTNAG 7 cut(s) 103, 314, 355, 896, 968, 1020, 1083
HpySE526I ACGT 2 cut(s) 132, 909
Hsp92I GRCGYC 2 cut(s) 132, 174
Hsp92II CATG 9 cut(s) 150, 205, 226, 416, 647, 692, 806, 818, 992
HspAI GCGC 3 cut(s) 460, 546, 686
KroI GCCGGC 1 cut(s) 176
KroNI GCCGGC 1 cut(s) 178
KspI CCGCGG 2 cut(s) 612, 637
Kzo9I GATC 1 cut(s) 360
LmnI GCTCC 2 cut(s) 208, 457
LweI GCATC 2 cut(s) 112, 379
MaeI CTAG 3 cut(s) 170, 191, 372
MaeII ACGT 2 cut(s) 132, 909
MaeIII GTNAC 3 cut(s) 325, 422, 506
MalI GATC 1 cut(s) 362
MbiI CCGCTC 1 cut(s) 472
MboI GATC 1 cut(s) 360
MboII GAAGA 6 cut(s) 22, 25, 28, 31, 259, 1005
MhlI GDGCHC 1 cut(s) 213
MluCI AATT 6 cut(s) 247, 278, 946, 976, 1062, 1087
MlyI GAGTC 3 cut(s) 95, 293, 793
MmeI TCCRAC 3 cut(s) 153, 186, 276
MroNI GCCGGC 1 cut(s) 176
MroXI GAANNNNTTC 1 cut(s) 659
MseI TTAA 4 cut(s) 116, 618, 1037, 1061
MspA1I CMGCKG 3 cut(s) 198, 611, 636
MspI CCGG 2 cut(s) 98, 177
MvnI CGCG 4 cut(s) 546, 556, 611, 636
MwoI GCNNNNNNNGC 5 cut(s) 83, 186, 195, 319, 413
NaeI GCCGGC 1 cut(s) 178
NcoI CCATGG 1 cut(s) 643
NdeI CATATG 1 cut(s) 44
NdeII GATC 1 cut(s) 360
NgoMIV GCCGGC 1 cut(s) 176
NlaIII CATG 9 cut(s) 150, 205, 226, 416, 647, 692, 806, 818, 992
NlaIV GGNNCC 2 cut(s) 167, 1005
NmeAIII GCCGAG 1 cut(s) 474
NmuCI GTSAC 1 cut(s) 422
NspI RCATGY 4 cut(s) 205, 226, 416, 692
PaeI GCATGC 2 cut(s) 416, 692
PagI TCATGA 1 cut(s) 802
PceI AGGCCT 1 cut(s) 1071
PdiI GCCGGC 1 cut(s) 178
PdmI GAANNNNTTC 1 cut(s) 659
PfeI GAWTC 3 cut(s) 90, 734, 788
PkrI GCNGC 3 cut(s) 200, 545, 638
PleI GAGTC 3 cut(s) 95, 292, 793
PpsI GAGTC 3 cut(s) 95, 292, 793
PshBI ATTAAT 1 cut(s) 1061
Psp124BI GAGCTC 1 cut(s) 213
PspN4I GGNNCC 2 cut(s) 167, 1005
PspPI GGNCC 4 cut(s) 289, 741, 879, 1003
PstNI CAGNNNCTG 1 cut(s) 110
RsaI GTAC 5 cut(s) 20, 108, 819, 860, 932
RsaNI GTAC 5 cut(s) 19, 107, 818, 859, 931
SacI GAGCTC 1 cut(s) 213
SacII CCGCGG 2 cut(s) 612, 637
SaqAI TTAA 4 cut(s) 116, 618, 1037, 1061
SatI GCNGC 3 cut(s) 199, 544, 637
Sau3AI GATC 1 cut(s) 360
Sau96I GGNCC 4 cut(s) 289, 741, 879, 1003
SchI GAGTC 3 cut(s) 95, 293, 793
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 2 cut(s) 112, 379
Sfr303I CCGCGG 2 cut(s) 612, 637
SgrBI CCGCGG 2 cut(s) 612, 637
SinI GGWCC 2 cut(s) 879, 1003
SmlI CTYRAG 2 cut(s) 160, 212
SmoI CTYRAG 2 cut(s) 160, 212
SphI GCATGC 2 cut(s) 416, 692
Sse9I AATT 6 cut(s) 247, 278, 946, 976, 1062, 1087
SseBI AGGCCT 1 cut(s) 1071
SspMI CTAG 3 cut(s) 170, 191, 372
SstI GAGCTC 1 cut(s) 213
StuI AGGCCT 1 cut(s) 1071
StyI CCWWGG 1 cut(s) 643
TaaI ACNGT 2 cut(s) 502, 935
TaiI ACGT 2 cut(s) 135, 912
TaqI TCGA 3 cut(s) 270, 529, 663
TaqII GACCGA 1 cut(s) 829
TasI AATT 6 cut(s) 247, 278, 946, 976, 1062, 1087
TatI WGTACW 1 cut(s) 817
TauI GCSGC 3 cut(s) 201, 546, 639
TfiI GAWTC 3 cut(s) 90, 734, 788
Tru1I TTAA 4 cut(s) 116, 618, 1037, 1061
Tru9I TTAA 4 cut(s) 116, 618, 1037, 1061
TscAI CASTG 1 cut(s) 349
TseFI GTSAC 1 cut(s) 422
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 1 cut(s) 31
TspGWI ACGGA 1 cut(s) 773
TspRI CASTG 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 879, 1003
VspI ATTAAT 1 cut(s) 1061
XagI CCTNNNNNAGG 1 cut(s) 725
XapI RAATTY 2 cut(s) 278, 976
XceI RCATGY 4 cut(s) 205, 226, 416, 692
XmnI GAANNNNTTC 1 cut(s) 659
XspI CTAG 3 cut(s) 170, 191, 372
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.