Rw7G018000

Belongs to the peptidase M10A family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0015037
Physical Location & Seq
Reverse (-)
19169597 .. 19170697
1101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G018000.1

Sequence Viewer

Length: 1101 bp
ATGTTTCCATTTCTCTGTTACCACTCTTCGTTCTCCTTCATATTATTCTTTTGCTTACTCTTTCTTTCCCGCCCATGCTTTCCCGCCAGAATCCTACCCGACCCTGCGGTAACAGTCATAACTGCGACGGCCAAAGTGTCCCACAACGCCACGTGGCACGACTTCACGAAGTTCCTGGACGCCGGGAAGGGCAGCCAGGTGGTCGGCATGTCGGAGCTCAAGAAGTACTTCAGCCGTTTCGGCTACTTGCCGGTGCCCGAAAAGACCAACTTCACCGACGTCTTCGACAAGCAGCTCGAATCGGCCCTGACTCTATACCAATCCAGGCTGAGCTTGCCCGTCACGGGAAAGCTTGACCCGGAAACCATTTCGGCCATCACTTCACCGAGATGCGGGGTCAGCGACGCGCCGCAGCAGCAACACACGCCCGCGAAGCTGCATGCGACGCGTCGTTATGCTTACTTCAACGGGAAGCCTCGCTGGGCTCCGGCGAAGTTGCTCACGTATGCCATCAACCCCAAACACGTTATCAGCTACGTGAGCTCGTCGGACATAGAGACAATATTCCACCGCGCTTTTTCTCGGTGGTCGGCTGTGATTCCGGTGAACTTCACCAAAGCCAGGGACTACTCCTCCGCAGACATCAAAATCGGGTTTTACAAAGGTGATCACGGCGACGGTGAGCCGTTCGATGGTGTCCTGGGTGTTCTAGCTCACGCTTTCTCTCCCGAGAACGGGAGGTTCCACCTCGACGCAGAGGAATCATGGGCCGTCGATTTCGAATCCGACAAGTCGAAGGTGGCCGTGGATTTGGACTCCGTGGCGACCCATGAGATTGGGCATCTTCTTGGGTTGGCTCACTCCTCGGTCAAGGATGCTGTCATGTACCCGAGTCTGAGTCCTAGGACTAAGAAAGTGGACCTCCAAATGGATGACGTGGCAGGCGTTCAGGCACTCTATGGGTCAAATCCTAATTTCAAATTTAGCTCATTGCAGTCGGAAAATTCTTACAACCACGCAGTTACTAATTTGGACACCACATTTGCGTCAAAGTGGACAATTTCTCTATCACTCATCACTTTGATTTTTCTCTTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

366

Amino Acids

40.78

Weight (kDa)

7.24

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 71 - 126 1.3e-11 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 161 - 321 1.7e-51 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16640
fragaria_vesca FvH4_5g19070
malus_domestica MD04G1063000.v1.1 MD06G1057300.v1.1
prunus_persica Prupe.5G068200_v2.0.a1
pyrus_communis pycom04g05670
rosa_chinensis RchiOBHm_Chr7g0203521
rosa_laevigata RLG00000003523
rosa_multiflora Rmu_sc0022597.1_g000010
rosa_roxburghii Rroxscaffold_3G00253740
rosa_rugosa Rorug07G0076400 Rorug07G0076500
rosa_samantha Rh7AG205900 Rh7CG218700 Rh7DG213100
rosa_wichuraiana Rw7G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 253
AccII CGCG 4 cut(s) 407, 431, 448, 573
AciI CCGC 8 cut(s) 70, 84, 107, 393, 410, 429, 571, 636
AcoI YGGCCR 3 cut(s) 129, 372, 801
AcsI RAATTY 2 cut(s) 980, 1003
AcuI CTGAAG 1 cut(s) 214
AcvI CACGTG 1 cut(s) 153
AcyI GRCGYC 2 cut(s) 180, 279
AfaI GTAC 2 cut(s) 227, 887
AfiI CCNNNNNNNGG 7 cut(s) 344, 392, 621, 692, 734, 735, 928
AflIII ACRYGT 2 cut(s) 446, 523
AgsI TTSAA 2 cut(s) 466, 979
AjiI CACGTC 1 cut(s) 937
AjnI CCWGG 5 cut(s) 174, 195, 323, 620, 699
AluBI AGCT 9 cut(s) 217, 295, 333, 352, 436, 534, 543, 713, 987
AluI AGCT 9 cut(s) 217, 295, 333, 352, 436, 534, 543, 713, 987
Alw21I GWGCWC 2 cut(s) 219, 545
Alw26I GTCTC 1 cut(s) 551
Ama87I CYCGRG 2 cut(s) 728, 889
AoxI GGCC 5 cut(s) 129, 303, 372, 768, 801
ApeKI GCWGC 5 cut(s) 192, 292, 412, 415, 436
ApoI RAATTY 2 cut(s) 980, 1003
ArsI GACNNNNNNTTYG 2 cut(s) 1025, 1057
Asp700I GAANNNNTTC 1 cut(s) 227
AspA2I CCTAGG 1 cut(s) 902
AspLEI GCGC 2 cut(s) 409, 575
AspS9I GGNCC 3 cut(s) 304, 768, 919
AsuC2I CCSGG 2 cut(s) 184, 359
AsuHPI GGTGA 6 cut(s) 265, 375, 604, 616, 677, 692
AsuII TTCGAA 1 cut(s) 780
AvaI CYCGRG 2 cut(s) 728, 889
AvaII GGWCC 1 cut(s) 919
AvrII CCTAGG 1 cut(s) 902
BaeGI GKGCMC 1 cut(s) 258
BanI GGYRCC 1 cut(s) 253
BanII GRGCYC 3 cut(s) 219, 487, 545
BbrPI CACGTG 1 cut(s) 153
BbsI GAAGAC 1 cut(s) 274
Bbv12I GWGCWC 2 cut(s) 219, 545
BbvI GCAGC 5 cut(s) 204, 304, 423, 424, 427
BccI CCATC 3 cut(s) 383, 518, 686
BceAI ACGGC 6 cut(s) 144, 219, 670, 688, 755, 788
BciT130I CCWGG 5 cut(s) 176, 197, 325, 622, 701
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 2 cut(s) 184, 359
BcoDI GTCTC 1 cut(s) 551
BfaI CTAG 2 cut(s) 710, 903
BglI GCCNNNNNGGC 1 cut(s) 240
BisI GCNGC 6 cut(s) 193, 293, 410, 413, 416, 437
BlnI CCTAGG 1 cut(s) 902
BlpI GCTNAGC 1 cut(s) 329
BlsI GCNGC 6 cut(s) 194, 294, 411, 414, 417, 438
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 7 cut(s) 176, 184, 197, 325, 359, 622, 701
Bme18I GGWCC 1 cut(s) 919
BmeT110I CYCGRG 2 cut(s) 728, 889
BmgBI CACGTC 1 cut(s) 937
BmgT120I GGNCC 3 cut(s) 304, 768, 919
BmiI GGNNCC 3 cut(s) 255, 486, 743
BmrFI CCNGG 7 cut(s) 176, 184, 197, 325, 359, 622, 701
BmsI GCATC 3 cut(s) 380, 850, 865
BpiI GAAGAC 1 cut(s) 274
Bpu1102I GCTNAGC 1 cut(s) 329
Bpu14I TTCGAA 1 cut(s) 780
BpuEI CTTGAG 1 cut(s) 203
BpuMI CCSGG 2 cut(s) 184, 359
BsaAI YACGTR 3 cut(s) 153, 504, 538
BsaHI GRCGYC 2 cut(s) 180, 279
BsaJI CCNNGG 6 cut(s) 621, 700, 804, 819, 864, 902
BsaWI WCCGGW 1 cut(s) 601
BsaXI ACNNNNNCTCC 2 cut(s) 617, 647
Bsc4I CCNNNNNNNGG 7 cut(s) 344, 392, 621, 692, 734, 735, 928
Bse118I RCCGGY 1 cut(s) 250
Bse3DI GCAATG 1 cut(s) 989
BseBI CCWGG 5 cut(s) 176, 197, 325, 622, 701
BseDI CCNNGG 6 cut(s) 621, 700, 804, 819, 864, 902
BseGI GGATG 2 cut(s) 880, 937
BseLI CCNNNNNNNGG 7 cut(s) 344, 392, 621, 692, 734, 735, 928
BseMI GCAATG 1 cut(s) 989
BseMII CTCAG 2 cut(s) 320, 887
BseRI GAGGAG 2 cut(s) 622, 853
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 5 cut(s) 204, 304, 423, 424, 427
BseYI CCCAGC 1 cut(s) 480
Bsh1236I CGCG 4 cut(s) 407, 431, 448, 573
BshFI GGCC 5 cut(s) 131, 305, 374, 770, 803
BshNI GGYRCC 1 cut(s) 253
BsiHKAI GWGCWC 2 cut(s) 219, 545
BsiHKCI CYCGRG 2 cut(s) 728, 889
BsiSI CCGG 5 cut(s) 183, 251, 359, 488, 602
BslFI GGGAC 2 cut(s) 124, 638
BslI CCNNNNNNNGG 7 cut(s) 344, 392, 621, 692, 734, 735, 928
BsmAI GTCTC 1 cut(s) 551
BsmFI GGGAC 2 cut(s) 124, 638
BsnI GGCC 5 cut(s) 131, 305, 374, 770, 803
BsoBI CYCGRG 2 cut(s) 728, 889
Bsp119I TTCGAA 1 cut(s) 780
Bsp1286I GDGCHC 4 cut(s) 219, 258, 487, 545
Bsp143I GATC 1 cut(s) 667
Bsp1720I GCTNAGC 1 cut(s) 329
BspACI CCGC 8 cut(s) 70, 84, 107, 393, 410, 429, 571, 636
BspANI GGCC 5 cut(s) 131, 305, 374, 770, 803
BspCNI CTCAG 2 cut(s) 321, 888
BspFNI CGCG 4 cut(s) 407, 431, 448, 573
BspLI GGNNCC 3 cut(s) 255, 486, 743
BspT104I TTCGAA 1 cut(s) 780
BspT107I GGYRCC 1 cut(s) 253
BsrDI GCAATG 1 cut(s) 989
BsrFI RCCGGY 1 cut(s) 250
BssAI RCCGGY 1 cut(s) 250
BssECI CCNNGG 6 cut(s) 621, 700, 804, 819, 864, 902
BssMI GATC 1 cut(s) 667
BssNI GRCGYC 2 cut(s) 180, 279
BssT1I CCWWGG 1 cut(s) 902
Bst2UI CCWGG 5 cut(s) 176, 197, 325, 622, 701
Bst4CI ACNGT 2 cut(s) 115, 680
Bst6I CTCTTC 1 cut(s) 31
BstACI GRCGYC 2 cut(s) 180, 279
BstBAI YACGTR 3 cut(s) 153, 504, 538
BstBI TTCGAA 1 cut(s) 780
BstC8I GCNNGC 4 cut(s) 335, 429, 441, 943
BstDEI CTNAG 3 cut(s) 329, 896, 909
BstDSI CCRYGG 2 cut(s) 804, 819
BstF5I GGATG 2 cut(s) 880, 937
BstFNI CGCG 4 cut(s) 407, 431, 448, 573
BstHHI GCGC 2 cut(s) 409, 575
BstKTI GATC 1 cut(s) 670
BstMAI GTCTC 1 cut(s) 551
BstMBI GATC 1 cut(s) 667
BstMWI GCNNNNNNNGC 8 cut(s) 240, 334, 399, 415, 424, 433, 445, 540
BstNI CCWGG 5 cut(s) 176, 197, 325, 622, 701
BstNSI RCATGY 2 cut(s) 211, 443
BstSCI CCNGG 7 cut(s) 174, 182, 195, 323, 357, 620, 699
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 4 cut(s) 407, 431, 448, 573
BstV1I GCAGC 5 cut(s) 204, 304, 423, 424, 427
BstV2I GAAGAC 1 cut(s) 274
BstXI CCANNNNNNTGG 1 cut(s) 836
BsuRI GGCC 5 cut(s) 131, 305, 374, 770, 803
BtgI CCRYGG 2 cut(s) 804, 819
BtrI CACGTC 1 cut(s) 937
BtsCI GGATG 2 cut(s) 880, 937
Cac8I GCNNGC 4 cut(s) 335, 429, 441, 943
CfoI GCGC 2 cut(s) 409, 575
Cfr10I RCCGGY 1 cut(s) 250
Cfr13I GGNCC 3 cut(s) 304, 768, 919
CseI GACGC 6 cut(s) 188, 413, 437, 454, 761, 1035
Csp6I GTAC 2 cut(s) 226, 886
CviAII CATG 6 cut(s) 75, 208, 440, 765, 830, 883
CviQI GTAC 2 cut(s) 226, 886
DdeI CTNAG 3 cut(s) 329, 896, 909
DpnI GATC 1 cut(s) 669
DpnII GATC 1 cut(s) 667
EaeI YGGCCR 3 cut(s) 129, 372, 801
Eam1104I CTCTTC 1 cut(s) 31
EarI CTCTTC 1 cut(s) 31
Ecl136II GAGCTC 2 cut(s) 217, 543
Eco130I CCWWGG 1 cut(s) 902
Eco24I GRGCYC 3 cut(s) 219, 487, 545
Eco47I GGWCC 1 cut(s) 919
Eco53kI GAGCTC 2 cut(s) 217, 543
Eco57I CTGAAG 1 cut(s) 214
Eco72I CACGTG 1 cut(s) 153
Eco88I CYCGRG 2 cut(s) 728, 889
EcoICRI GAGCTC 2 cut(s) 217, 543
EcoRII CCWGG 5 cut(s) 174, 195, 323, 620, 699
EcoT14I CCWWGG 1 cut(s) 902
EcoT38I GRGCYC 3 cut(s) 219, 487, 545
ErhI CCWWGG 1 cut(s) 902
FaeI CATG 6 cut(s) 78, 211, 443, 768, 833, 886
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 254, 286
FaqI GGGAC 2 cut(s) 124, 638
FatI CATG 6 cut(s) 74, 207, 439, 764, 829, 882
FauI CCCGC 4 cut(s) 77, 91, 386, 436
FbaI TGATCA 1 cut(s) 667
Fnu4HI GCNGC 6 cut(s) 193, 293, 410, 413, 416, 437
FokI GGATG 2 cut(s) 887, 944
FriOI GRGCYC 3 cut(s) 219, 487, 545
Fsp4HI GCNGC 6 cut(s) 193, 293, 410, 413, 416, 437
FspBI CTAG 2 cut(s) 710, 903
GlaI GCGC 2 cut(s) 408, 574
GluI GCNGC 6 cut(s) 193, 293, 410, 413, 416, 437
GsaI CCCAGC 1 cut(s) 484
HaeIII GGCC 5 cut(s) 131, 305, 374, 770, 803
HapII CCGG 5 cut(s) 183, 251, 359, 488, 602
HgaI GACGC 6 cut(s) 188, 413, 437, 454, 761, 1035
HhaI GCGC 2 cut(s) 409, 575
Hin1I GRCGYC 2 cut(s) 180, 279
Hin1II CATG 6 cut(s) 78, 211, 443, 768, 833, 886
Hin6I GCGC 2 cut(s) 407, 573
HinP1I GCGC 2 cut(s) 407, 573
HindIII AAGCTT 1 cut(s) 350
HinfI GANTC 9 cut(s) 90, 299, 310, 598, 761, 782, 815, 892, 898
HpaII CCGG 5 cut(s) 183, 251, 359, 488, 602
HphI GGTGA 6 cut(s) 265, 375, 604, 616, 677, 692
Hpy166II GTNNAC 3 cut(s) 607, 919, 1056
Hpy188I TCNGA 5 cut(s) 214, 550, 787, 897, 1000
Hpy188III TCNNGA 3 cut(s) 166, 220, 728
Hpy8I GTNNAC 3 cut(s) 607, 919, 1056
Hpy99I CGWCG 9 cut(s) 130, 281, 407, 448, 453, 550, 680, 755, 776
HpyAV CCTTC 3 cut(s) 46, 181, 790
HpyCH4III ACNGT 2 cut(s) 115, 680
HpyCH4IV ACGT 6 cut(s) 152, 279, 503, 525, 537, 936
HpyCH4V TGCA 2 cut(s) 439, 994
HpyF10VI GCNNNNNNNGC 8 cut(s) 240, 334, 399, 415, 424, 433, 445, 540
HpyF3I CTNAG 3 cut(s) 329, 896, 909
HpySE526I ACGT 6 cut(s) 152, 279, 503, 525, 537, 936
Hsp92I GRCGYC 2 cut(s) 180, 279
Hsp92II CATG 6 cut(s) 78, 211, 443, 768, 833, 886
HspAI GCGC 2 cut(s) 407, 573
Ksp22I TGATCA 1 cut(s) 667
Kzo9I GATC 1 cut(s) 667
LmnI GCTCC 2 cut(s) 214, 490
Lsp1109I GCAGC 5 cut(s) 204, 304, 423, 424, 427
LweI GCATC 3 cut(s) 380, 850, 865
MaeI CTAG 2 cut(s) 710, 903
MaeII ACGT 6 cut(s) 152, 279, 503, 525, 537, 936
MaeIII GTNAC 4 cut(s) 17, 109, 340, 1021
MalI GATC 1 cut(s) 669
MboI GATC 1 cut(s) 667
MboII GAAGA 3 cut(s) 18, 274, 836
MhlI GDGCHC 4 cut(s) 219, 258, 487, 545
MluCI AATT 5 cut(s) 973, 980, 1003, 1027, 1059
MluI ACGCGT 1 cut(s) 446
MlyI GAGTC 4 cut(s) 304, 809, 901, 907
MmeI TCCRAC 4 cut(s) 192, 528, 810, 978
MnlI CCTC 7 cut(s) 486, 643, 732, 751, 758, 874, 932
MroXI GAANNNNTTC 1 cut(s) 227
MslI CAYNNNNRTG 1 cut(s) 388
MspI CCGG 5 cut(s) 183, 251, 359, 488, 602
MspR9I CCNGG 7 cut(s) 176, 184, 197, 325, 359, 622, 701
MvaI CCWGG 5 cut(s) 176, 197, 325, 622, 701
MvnI CGCG 4 cut(s) 407, 431, 448, 573
MwoI GCNNNNNNNGC 8 cut(s) 240, 334, 399, 415, 424, 433, 445, 540
NciI CCSGG 2 cut(s) 184, 359
NdeII GATC 1 cut(s) 667
NlaIII CATG 6 cut(s) 78, 211, 443, 768, 833, 886
NlaIV GGNNCC 3 cut(s) 255, 486, 743
NmuCI GTSAC 1 cut(s) 340
NspI RCATGY 2 cut(s) 211, 443
NspV TTCGAA 1 cut(s) 780
PaeI GCATGC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 942
PdmI GAANNNNTTC 1 cut(s) 227
PfeI GAWTC 5 cut(s) 90, 299, 598, 761, 782
PfoI TCCNGGA 1 cut(s) 174
PkrI GCNGC 6 cut(s) 194, 294, 411, 414, 417, 438
PleI GAGTC 4 cut(s) 304, 809, 900, 906
PmaCI CACGTG 1 cut(s) 153
PmlI CACGTG 1 cut(s) 153
PpsI GAGTC 4 cut(s) 304, 809, 900, 906
Ppu21I YACGTR 3 cut(s) 153, 504, 538
Psp124BI GAGCTC 2 cut(s) 219, 545
Psp6I CCWGG 5 cut(s) 174, 195, 323, 620, 699
PspCI CACGTG 1 cut(s) 153
PspFI CCCAGC 1 cut(s) 480
PspGI CCWGG 5 cut(s) 174, 195, 323, 620, 699
PspN4I GGNNCC 3 cut(s) 255, 486, 743
PspPI GGNCC 3 cut(s) 304, 768, 919
RsaI GTAC 2 cut(s) 227, 887
RsaNI GTAC 2 cut(s) 226, 886
RseI CAYNNNNRTG 1 cut(s) 388
SacI GAGCTC 2 cut(s) 219, 545
SatI GCNGC 6 cut(s) 193, 293, 410, 413, 416, 437
Sau3AI GATC 1 cut(s) 667
Sau96I GGNCC 3 cut(s) 304, 768, 919
ScaI AGTACT 1 cut(s) 227
SchI GAGTC 4 cut(s) 304, 809, 901, 907
ScrFI CCNGG 7 cut(s) 176, 184, 197, 325, 359, 622, 701
SduI GDGCHC 4 cut(s) 219, 258, 487, 545
SfaNI GCATC 3 cut(s) 380, 850, 865
SfuI TTCGAA 1 cut(s) 780
SinI GGWCC 1 cut(s) 919
SmiMI CAYNNNNRTG 1 cut(s) 388
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
SphI GCATGC 1 cut(s) 443
Sse9I AATT 5 cut(s) 973, 980, 1003, 1027, 1059
SsiI CCGC 8 cut(s) 70, 84, 107, 393, 410, 429, 571, 636
SspI AATATT 1 cut(s) 564
SspMI CTAG 2 cut(s) 710, 903
SstI GAGCTC 2 cut(s) 219, 545
StyD4I CCNGG 7 cut(s) 174, 182, 195, 323, 357, 620, 699
StyI CCWWGG 1 cut(s) 902
TaaI ACNGT 2 cut(s) 115, 680
TaiI ACGT 6 cut(s) 155, 282, 506, 528, 540, 939
TaqI TCGA 7 cut(s) 285, 297, 690, 750, 774, 780, 794
TaqII GACCGA 1 cut(s) 856
TasI AATT 5 cut(s) 973, 980, 1003, 1027, 1059
TatI WGTACW 1 cut(s) 225
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 5 cut(s) 90, 299, 598, 761, 782
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 5 cut(s) 192, 292, 412, 415, 436
Tsp45I GTSAC 1 cut(s) 340
TspDTI ATGAA 1 cut(s) 28
TspGWI ACGGA 1 cut(s) 808
VpaK11BI GGWCC 1 cut(s) 919
XapI RAATTY 2 cut(s) 980, 1003
XceI RCATGY 2 cut(s) 211, 443
XmaJI CCTAGG 1 cut(s) 902
XmnI GAANNNNTTC 1 cut(s) 227
XspI CTAG 2 cut(s) 710, 903
ZraI GACGTC 1 cut(s) 280
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.