pycom04g05670

Belongs to the peptidase M10A family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0015037
Physical Location & Seq
Forward (+)
5457152 .. 5458243
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g05670.1

Sequence Viewer

Length: 1092 bp
ATGTTCCCATTTTTCAGGTACAACTCTTTCTTCTTCTTCATATGCCTCTGCTTCCTCTGTTTTTCCCGCCCTTCCTTTCCAGCCAGAATTTTACCGGACTCAGTAACTGTATTAACCGCCGCATCCGACGTCCACAACGCCACATGGCACGACTTCTCAAGGTTTCTAGACGCCGGGAAAGGAAGCCTAGTCAGTGGCATGTCCGAGCTTAAGAACTACTTCCACCGTTTTGGCTACTTACCCAATTCCAACTTCACTGATTTTTTTGACATAGAACTTGAGTCCGCCTTGACGCTATACCAGTCCAAGCTGAGCTTGCCCATCACAGGCAAGCTGGACACGGACACCATCTCGGCGATCATTTCACCGAGGTGTGGTGTCAGTGACACGTCGCAACATACGGCTCTGCATGCGACGCGTCACTTCGCCTACTTCTACGGCAAGCCGAGGTGGGCGCGCCCTTCCCCCGTATCGCTCACATACGCTTTCTCCCCCAATCACATGGTCAGTTATCTGAACCCATCAGATGTTCGAGACATTTTTCACCGCGCTTTTTCGCGGTGGTCGGCGGTGATCCCTGTGAACTTCACTGAGACCGACGACTACGCTTCCGCGGATATCAAAATAGGGTTTTACCGCGGCGACCACGGTGACGGGGAGCCGTTCGACGGGGTGTTGGGGGTGCTGGGGCATGCTTTTTCGCCCGAGAACGGGAGGTTCCACCTGGACGCGGCGGAATCGTGGGCCGTTGATTTCAAAACGGATAAGTCAAAGGTGGCGATTGATTTGGAATCGGTGGCGACTCATGAGATAGGGCATGTACTCGGGCTGGCTCACACCTCGGTCAAGGAGGCCGTTATGTACCCGAGCCTGAGTCCTCGGACCAAGAAGGTGGACCTCAAGATTGATGACGTGGAAGGTGTCCAAGCTTTGTATGGGTCAAACCCTAATTTCAAGTTTAGCTCATTGCAGAGTGAAAATTCTTACAATCATGCGGTTTCTTTGGAGTCAAGGTCTTCCTCTAAGTGGTCCATTTTTTTAACACTCACCACCACTTTGGTATTGCTCTTAGGCCTTAATTTGGCCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

364

Amino Acids

40.54

Weight (kDa)

6.19

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 67 - 120 1.1e-08 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 155 - 313 3.6e-51 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16640
fragaria_vesca FvH4_5g19070
malus_domestica MD04G1063000.v1.1 MD06G1057300.v1.1
prunus_persica Prupe.5G068200_v2.0.a1
pyrus_communis pycom04g05670
rosa_chinensis RchiOBHm_Chr7g0203521
rosa_laevigata RLG00000003523
rosa_multiflora Rmu_sc0022597.1_g000010
rosa_roxburghii Rroxscaffold_3G00253740
rosa_rugosa Rorug07G0076400 Rorug07G0076500
rosa_samantha Rh7AG205900 Rh7CG218700 Rh7DG213100
rosa_wichuraiana Rw7G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 132
AccII CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
AclWI GGATC 1 cut(s) 568
AcsI RAATTY 2 cut(s) 87, 979
AcyI GRCGYC 2 cut(s) 129, 171
AfaI GTAC 3 cut(s) 20, 822, 863
AfiI CCNNNNNNNGG 8 cut(s) 326, 374, 668, 710, 711, 730, 1026, 1081
AflII CTTAAG 1 cut(s) 209
AflIII ACRYGT 2 cut(s) 387, 416
AgsI TTSAA 2 cut(s) 757, 955
AjiI CACGTC 2 cut(s) 390, 913
AjnI CCWGG 1 cut(s) 723
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 6 cut(s) 208, 310, 315, 334, 929, 963
AluI AGCT 6 cut(s) 208, 310, 315, 334, 929, 963
Alw26I GTCTC 2 cut(s) 528, 587
AlwI GGATC 1 cut(s) 568
AlwNI CAGNNNCTG 1 cut(s) 107
Ama87I CYCGRG 3 cut(s) 704, 824, 865
AoxI GGCC 4 cut(s) 744, 852, 1072, 1083
ApoI RAATTY 2 cut(s) 87, 979
AscI GGCGCGCC 1 cut(s) 455
Asp700I GAANNNNTTC 1 cut(s) 218
AspLEI GCGC 3 cut(s) 457, 459, 551
AspS9I GGNCC 4 cut(s) 744, 882, 895, 1029
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 5 cut(s) 357, 536, 583, 662, 1039
AvaI CYCGRG 3 cut(s) 704, 824, 865
AvaII GGWCC 3 cut(s) 882, 895, 1029
BbsI GAAGAC 1 cut(s) 1008
BccI CCATC 3 cut(s) 329, 356, 529
BceAI ACGGC 5 cut(s) 417, 454, 646, 731, 839
BciT130I CCWGG 1 cut(s) 725
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 2 cut(s) 528, 587
BfaI CTAG 2 cut(s) 167, 188
BfrI CTTAAG 1 cut(s) 209
BisI GCNGC 3 cut(s) 120, 640, 732
BlpI GCTNAGC 1 cut(s) 311
BlsI GCNGC 3 cut(s) 121, 641, 733
Bme1390I CCNGG 2 cut(s) 175, 725
Bme18I GGWCC 3 cut(s) 882, 895, 1029
BmeT110I CYCGRG 3 cut(s) 704, 824, 865
BmgBI CACGTC 2 cut(s) 390, 913
BmgT120I GGNCC 4 cut(s) 744, 882, 895, 1029
BmiI GGNNCC 2 cut(s) 660, 719
BmrFI CCNGG 2 cut(s) 175, 725
BmsI GCATC 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 1008
Bpu1102I GCTNAGC 1 cut(s) 311
BpuEI CTTGAG 3 cut(s) 142, 299, 884
BpuMI CCSGG 1 cut(s) 175
BsaHI GRCGYC 2 cut(s) 129, 171
BsaI GGTCTC 1 cut(s) 587
BsaJI CCNNGG 7 cut(s) 368, 446, 612, 637, 646, 840, 878
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 4 cut(s) 473, 503, 998, 1028
Bsc4I CCNNNNNNNGG 8 cut(s) 326, 374, 668, 710, 711, 730, 1026, 1081
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 965
BseBI CCWGG 1 cut(s) 725
BseDI CCNNGG 7 cut(s) 368, 446, 612, 637, 646, 840, 878
BseGI GGATG 1 cut(s) 122
BseLI CCNNNNNNNGG 8 cut(s) 326, 374, 668, 710, 711, 730, 1026, 1081
BseMI GCAATG 1 cut(s) 965
BseMII CTCAG 4 cut(s) 114, 302, 582, 863
BseNI ACTGG 1 cut(s) 301
BsePI GCGCGC 1 cut(s) 455
BseYI CCCAGC 1 cut(s) 685
Bsh1236I CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
BshFI GGCC 4 cut(s) 746, 854, 1074, 1085
BsiHKCI CYCGRG 3 cut(s) 704, 824, 865
BsiSI CCGG 2 cut(s) 95, 174
BslI CCNNNNNNNGG 8 cut(s) 326, 374, 668, 710, 711, 730, 1026, 1081
BsmAI GTCTC 2 cut(s) 528, 587
BsnI GGCC 4 cut(s) 746, 854, 1074, 1085
Bso31I GGTCTC 1 cut(s) 587
BsoBI CYCGRG 3 cut(s) 704, 824, 865
Bsp143I GATC 2 cut(s) 357, 573
Bsp1720I GCTNAGC 1 cut(s) 311
BspANI GGCC 4 cut(s) 746, 854, 1074, 1085
BspCNI CTCAG 4 cut(s) 113, 303, 583, 864
BspFNI CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
BspHI TCATGA 1 cut(s) 805
BspLI GGNNCC 2 cut(s) 660, 719
BspPI GGATC 1 cut(s) 568
BspTI CTTAAG 1 cut(s) 209
BspTNI GGTCTC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 965
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 7 cut(s) 368, 446, 612, 637, 646, 840, 878
BssHII GCGCGC 1 cut(s) 455
BssMI GATC 2 cut(s) 357, 573
BssNI GRCGYC 2 cut(s) 129, 171
Bst2UI CCWGG 1 cut(s) 725
Bst4CI ACNGT 3 cut(s) 109, 227, 650
BstACI GRCGYC 2 cut(s) 129, 171
BstAFI CTTAAG 1 cut(s) 209
BstC8I GCNNGC 7 cut(s) 317, 332, 411, 443, 457, 693, 831
BstDEI CTNAG 6 cut(s) 100, 311, 591, 872, 1023, 1069
BstDSI CCRYGG 3 cut(s) 612, 637, 646
BstF5I GGATG 1 cut(s) 122
BstFNI CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
BstHHI GCGC 3 cut(s) 457, 459, 551
BstKTI GATC 2 cut(s) 360, 576
BstMAI GTCTC 2 cut(s) 528, 587
BstMBI GATC 2 cut(s) 357, 573
BstMWI GCNNNNNNNGC 3 cut(s) 316, 410, 415
BstNI CCWGG 1 cut(s) 725
BstNSI RCATGY 4 cut(s) 202, 413, 695, 821
BstSCI CCNGG 2 cut(s) 173, 723
BstUI CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
BstV2I GAAGAC 1 cut(s) 1008
BstXI CCANNNNNNTGG 4 cut(s) 230, 502, 892, 1057
BsuRI GGCC 4 cut(s) 746, 854, 1074, 1085
BtgI CCRYGG 3 cut(s) 612, 637, 646
BtrI CACGTC 2 cut(s) 390, 913
BtsCI GGATG 1 cut(s) 122
BtsIMutI CAGTG 4 cut(s) 199, 255, 388, 588
Cac8I GCNNGC 7 cut(s) 317, 332, 411, 443, 457, 693, 831
CaiI CAGNNNCTG 1 cut(s) 107
CciI TCATGA 1 cut(s) 805
CfoI GCGC 3 cut(s) 457, 459, 551
Cfr13I GGNCC 4 cut(s) 744, 882, 895, 1029
Cfr42I CCGCGG 2 cut(s) 615, 640
CseI GACGC 5 cut(s) 179, 301, 407, 424, 737
Csp6I GTAC 3 cut(s) 19, 821, 862
CviAII CATG 8 cut(s) 144, 199, 410, 502, 692, 806, 818, 992
CviQI GTAC 3 cut(s) 19, 821, 862
DdeI CTNAG 6 cut(s) 100, 311, 591, 872, 1023, 1069
DpnI GATC 2 cut(s) 359, 575
DpnII GATC 2 cut(s) 357, 573
EciI GGCGGA 2 cut(s) 274, 749
Eco147I AGGCCT 1 cut(s) 1074
Eco31I GGTCTC 1 cut(s) 587
Eco32I GATATC 1 cut(s) 619
Eco47I GGWCC 3 cut(s) 882, 895, 1029
Eco88I CYCGRG 3 cut(s) 704, 824, 865
EcoRII CCWGG 1 cut(s) 723
EcoRV GATATC 1 cut(s) 619
FaeI CATG 8 cut(s) 147, 202, 413, 505, 695, 809, 821, 995
FalI AAGNNNNNCTT 4 cut(s) 203, 235, 299, 331
FatI CATG 8 cut(s) 143, 198, 409, 501, 691, 805, 817, 991
FauI CCCGC 1 cut(s) 74
FauNDI CATATG 1 cut(s) 41
Fnu4HI GCNGC 3 cut(s) 120, 640, 732
FokI GGATG 1 cut(s) 109
Fsp4HI GCNGC 3 cut(s) 120, 640, 732
FspBI CTAG 2 cut(s) 167, 188
GlaI GCGC 3 cut(s) 456, 458, 550
GluI GCNGC 3 cut(s) 120, 640, 732
GsaI CCCAGC 1 cut(s) 689
HaeIII GGCC 4 cut(s) 746, 854, 1074, 1085
HapII CCGG 2 cut(s) 95, 174
HgaI GACGC 5 cut(s) 179, 301, 407, 424, 737
HhaI GCGC 3 cut(s) 457, 459, 551
Hin1I GRCGYC 2 cut(s) 129, 171
Hin1II CATG 8 cut(s) 147, 202, 413, 505, 695, 809, 821, 995
Hin6I GCGC 3 cut(s) 455, 457, 549
HinP1I GCGC 3 cut(s) 455, 457, 549
HindIII AAGCTT 1 cut(s) 927
HinfI GANTC 7 cut(s) 98, 281, 737, 791, 802, 874, 1007
HpaII CCGG 2 cut(s) 95, 174
HphI GGTGA 5 cut(s) 357, 536, 583, 662, 1039
Hpy166II GTNNAC 3 cut(s) 133, 583, 895
Hpy188I TCNGA 5 cut(s) 127, 205, 516, 526, 882
Hpy188III TCNNGA 4 cut(s) 167, 533, 806, 901
Hpy8I GTNNAC 3 cut(s) 133, 583, 895
Hpy99I CGWCG 5 cut(s) 131, 394, 418, 602, 671
HpyAV CCTTC 4 cut(s) 81, 471, 883, 911
HpyCH4III ACNGT 3 cut(s) 109, 227, 650
HpyCH4IV ACGT 3 cut(s) 129, 389, 912
HpyCH4V TGCA 2 cut(s) 409, 970
HpyF10VI GCNNNNNNNGC 3 cut(s) 316, 410, 415
HpyF3I CTNAG 6 cut(s) 100, 311, 591, 872, 1023, 1069
HpySE526I ACGT 3 cut(s) 129, 389, 912
Hsp92I GRCGYC 2 cut(s) 129, 171
Hsp92II CATG 8 cut(s) 147, 202, 413, 505, 695, 809, 821, 995
HspAI GCGC 3 cut(s) 455, 457, 549
KspI CCGCGG 2 cut(s) 615, 640
Kzo9I GATC 2 cut(s) 357, 573
LmnI GCTCC 1 cut(s) 658
LweI GCATC 1 cut(s) 131
MaeI CTAG 2 cut(s) 167, 188
MaeII ACGT 3 cut(s) 129, 389, 912
MaeIII GTNAC 4 cut(s) 103, 383, 419, 650
MalI GATC 2 cut(s) 359, 575
MboI GATC 2 cut(s) 357, 573
MboII GAAGA 4 cut(s) 22, 25, 28, 1008
MluCI AATT 5 cut(s) 87, 244, 949, 979, 1078
MluI ACGCGT 1 cut(s) 416
MlyI GAGTC 5 cut(s) 92, 290, 796, 883, 1016
MmeI TCCRAC 2 cut(s) 150, 273
MroXI GAANNNNTTC 1 cut(s) 218
MseI TTAA 4 cut(s) 113, 210, 1040, 1077
MslI CAYNNNNRTG 1 cut(s) 370
MspA1I CMGCKG 2 cut(s) 614, 639
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 2 cut(s) 95, 174
MspR9I CCNGG 2 cut(s) 175, 725
MvaI CCWGG 1 cut(s) 725
MvnI CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
MwoI GCNNNNNNNGC 3 cut(s) 316, 410, 415
NciI CCSGG 1 cut(s) 175
NdeI CATATG 1 cut(s) 41
NdeII GATC 2 cut(s) 357, 573
NlaIII CATG 8 cut(s) 147, 202, 413, 505, 695, 809, 821, 995
NlaIV GGNNCC 2 cut(s) 660, 719
NmeAIII GCCGAG 2 cut(s) 332, 471
NmuCI GTSAC 3 cut(s) 383, 419, 650
NspI RCATGY 4 cut(s) 202, 413, 695, 821
OliI CACNNNNGTG 1 cut(s) 370
PaeI GCATGC 2 cut(s) 413, 695
PagI TCATGA 1 cut(s) 805
PalAI GGCGCGCC 1 cut(s) 455
PauI GCGCGC 1 cut(s) 455
PceI AGGCCT 1 cut(s) 1074
PdmI GAANNNNTTC 1 cut(s) 218
PfeI GAWTC 2 cut(s) 737, 791
PkrI GCNGC 3 cut(s) 121, 641, 733
PleI GAGTC 5 cut(s) 92, 289, 796, 882, 1015
PpsI GAGTC 5 cut(s) 92, 289, 796, 882, 1015
Psp6I CCWGG 1 cut(s) 723
PspFI CCCAGC 1 cut(s) 685
PspGI CCWGG 1 cut(s) 723
PspN4I GGNNCC 2 cut(s) 660, 719
PspPI GGNCC 4 cut(s) 744, 882, 895, 1029
PstNI CAGNNNCTG 1 cut(s) 107
PteI GCGCGC 1 cut(s) 455
RsaI GTAC 3 cut(s) 20, 822, 863
RsaNI GTAC 3 cut(s) 19, 821, 862
RseI CAYNNNNRTG 1 cut(s) 370
SacII CCGCGG 2 cut(s) 615, 640
SaqAI TTAA 4 cut(s) 113, 210, 1040, 1077
SatI GCNGC 3 cut(s) 120, 640, 732
Sau3AI GATC 2 cut(s) 357, 573
Sau96I GGNCC 4 cut(s) 744, 882, 895, 1029
SchI GAGTC 5 cut(s) 92, 290, 796, 883, 1016
ScrFI CCNGG 2 cut(s) 175, 725
SfaNI GCATC 1 cut(s) 131
Sfr303I CCGCGG 2 cut(s) 615, 640
SgrBI CCGCGG 2 cut(s) 615, 640
SgsI GGCGCGCC 1 cut(s) 455
SinI GGWCC 3 cut(s) 882, 895, 1029
SmiMI CAYNNNNRTG 1 cut(s) 370
SmlI CTYRAG 4 cut(s) 157, 209, 278, 899
SmoI CTYRAG 4 cut(s) 157, 209, 278, 899
SphI GCATGC 2 cut(s) 413, 695
Sse9I AATT 5 cut(s) 87, 244, 949, 979, 1078
SseBI AGGCCT 1 cut(s) 1074
SspMI CTAG 2 cut(s) 167, 188
StuI AGGCCT 1 cut(s) 1074
StyD4I CCNGG 2 cut(s) 173, 723
TaaI ACNGT 3 cut(s) 109, 227, 650
TaiI ACGT 3 cut(s) 132, 392, 915
TaqI TCGA 2 cut(s) 532, 666
TaqII GACCGA 2 cut(s) 611, 832
TasI AATT 5 cut(s) 87, 244, 949, 979, 1078
TatI WGTACW 1 cut(s) 820
TauI GCSGC 3 cut(s) 122, 642, 734
TfiI GAWTC 2 cut(s) 737, 791
Tru1I TTAA 4 cut(s) 113, 210, 1040, 1077
Tru9I TTAA 4 cut(s) 113, 210, 1040, 1077
TscAI CASTG 4 cut(s) 199, 262, 388, 595
TseFI GTSAC 3 cut(s) 383, 419, 650
Tsp45I GTSAC 3 cut(s) 383, 419, 650
TspDTI ATGAA 1 cut(s) 28
TspGWI ACGGA 2 cut(s) 356, 776
TspRI CASTG 4 cut(s) 199, 262, 388, 595
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 3 cut(s) 882, 895, 1029
XapI RAATTY 2 cut(s) 87, 979
XbaI TCTAGA 1 cut(s) 166
XceI RCATGY 4 cut(s) 202, 413, 695, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 932
XmnI GAANNNNTTC 1 cut(s) 218
XspI CTAG 2 cut(s) 167, 188
ZraI GACGTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.