FvH4_5g19070

Belongs to the peptidase M10A family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0015037
Physical Location & Seq
Reverse (-)
10983741 .. 10984868
1128 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g19070.t1

Sequence Viewer

Length: 1128 bp
ATGATCGGCTCACATCCCAAAATAACCAACACCATGCTTGCGGTTTCAAGTTACAACTCTTCGTTCTCCGTGTTATTCTTCTGCTTAGTCTTCCTTTCCCGCCCATGCTTTCCCGCCAGAATCCTACCCGACCCCGCAGTAACCGTCTTAACTGCCACCGCCAACGTGTCCCCCAACGCCGCGTGGCACGACTTCACGAAGTTCCTTGACGCCGGGAAGGGGAGCCAGCTGGTCGGCATGTCGGAGCTCAAGAAGTACTTCCACCGTTTCGGCTATTTACCGGTGCCTGACACGACCAACTTCACCGACCTCTTCGACAAGCAGTTTGAATCGGCTCTGACTCTATATCAATCCAAGCTGAGCTTGCCTGTCACGGGTAAGCTCGACTCGGACACCATCTCGGCCATCACCTCGCCGAGATGCGGCGTGAGCGACGCGGGGCTGCGCATGCCTGCTGCAAAGCTGCACACGACGCGTCGCTTCGCCTACTTCTACGGGAAGCCTCGTTGGGCTCCCGCGAAGTCGCTCACGTACGCCTTCAGCCCCAACCACGTAATCAGCTACGTGAGCTCGTCGGACATAGAGGCAATATTCCACCGCGCCTTCTCTCGGTGGTCTGCGGTGATTCCGGTCAACTTTACAAAAGCCAAAGACTATTCCTCCGCGGACATCAAAATCGGGTTTTACAAAGGCGATCACGGCGACGGCGAGCCGTTCGATGGTGTGTTGGGTGTTCTAGCTCACGCTTTCTCTCCCGAGAACGGGAGGTTCCACCTCGACGCGGCGGAAACTTGGGCCGTCGATTTCGAATCGGACAAGTCGAAGGTGGCCGTGGATTTGGAGTCCGTGGCAACGCATGAGATTGGGCATGTACTTGGGTTGGCTCACTCCTCGGTCAAGGAAGCTGTCATGTACCCGAGTCTGAGTCCTAGGACTAAGAAAGTGGACCTCAAGATGGATGACGTGGAAGGCGTTCAGGCACTGTACGGGTCAAACCCTAATTTCAAATATAGCTCATTGCAGTCGGAAAATTCTTACAACCACGCAGTTACTAATTTGGACACCACATTTGCTTCTAAGTGGACAATTTCTCTATCACTCATCACTTTGATTTTTCTCTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

376

Amino Acids

41.37

Weight (kDa)

7.22

Isoelectric Point (pI)

36.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 81 - 136 1.2e-09 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 170 - 330 1.7e-52 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16640
fragaria_vesca FvH4_5g19070
malus_domestica MD04G1063000.v1.1 MD06G1057300.v1.1
prunus_persica Prupe.5G068200_v2.0.a1
pyrus_communis pycom04g05670
rosa_chinensis RchiOBHm_Chr7g0203521
rosa_laevigata RLG00000003523
rosa_multiflora Rmu_sc0022597.1_g000010
rosa_roxburghii Rroxscaffold_3G00253740
rosa_rugosa Rorug07G0076400 Rorug07G0076500
rosa_samantha Rh7AG205900 Rh7CG218700 Rh7DG213100
rosa_wichuraiana Rw7G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 446
AccB1I GGYRCC 1 cut(s) 283
AccII CGCG 7 cut(s) 182, 437, 475, 518, 600, 665, 782
AcoI YGGCCR 2 cut(s) 402, 828
AcsI RAATTY 1 cut(s) 1030
AcuI CTGAAG 1 cut(s) 523
AcyI GRCGYC 1 cut(s) 210
AfaI GTAC 5 cut(s) 257, 533, 873, 914, 986
AfiI CCNNNNNNNGG 9 cut(s) 219, 374, 422, 609, 719, 761, 762, 781, 955
AflIII ACRYGT 2 cut(s) 165, 473
AgeI ACCGGT 1 cut(s) 280
AgsI TTSAA 3 cut(s) 48, 329, 1006
AjiI CACGTC 1 cut(s) 964
Alw21I GWGCWC 2 cut(s) 249, 572
AlwNI CAGNNNCTG 1 cut(s) 982
Ama87I CYCGRG 2 cut(s) 755, 916
AoxI GGCC 3 cut(s) 402, 795, 828
ApeKI GCWGC 3 cut(s) 442, 455, 463
ApoI RAATTY 1 cut(s) 1030
ArsI GACNNNNNNTTYG 4 cut(s) 308, 340, 1052, 1084
AsiGI ACCGGT 1 cut(s) 280
Asp700I GAANNNNTTC 2 cut(s) 257, 972
AspA2I CCTAGG 1 cut(s) 929
AspLEI GCGC 2 cut(s) 447, 602
AspS9I GGNCC 2 cut(s) 795, 946
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 3 cut(s) 295, 400, 634
AsuII TTCGAA 1 cut(s) 807
AvaI CYCGRG 2 cut(s) 755, 916
AvaII GGWCC 1 cut(s) 946
AvrII CCTAGG 1 cut(s) 929
BanI GGYRCC 1 cut(s) 283
BanII GRGCYC 3 cut(s) 249, 514, 572
BbsI GAAGAC 1 cut(s) 82
Bbv12I GWGCWC 2 cut(s) 249, 572
BbvI GCAGC 3 cut(s) 429, 442, 450
BccI CCATC 4 cut(s) 404, 413, 713, 949
BceAI ACGGC 5 cut(s) 697, 715, 721, 782, 815
BcnI CCSGG 1 cut(s) 214
BfaI CTAG 2 cut(s) 737, 930
BisI GCNGC 6 cut(s) 180, 424, 443, 456, 464, 783
BlnI CCTAGG 1 cut(s) 929
BlpI GCTNAGC 1 cut(s) 359
BlsI GCNGC 6 cut(s) 181, 425, 444, 457, 465, 784
BmcAI AGTACT 1 cut(s) 257
Bme1390I CCNGG 1 cut(s) 214
Bme18I GGWCC 1 cut(s) 946
BmeT110I CYCGRG 2 cut(s) 755, 916
BmgBI CACGTC 1 cut(s) 964
BmgT120I GGNCC 2 cut(s) 795, 946
BmiI GGNNCC 4 cut(s) 224, 285, 513, 770
BmrFI CCNGG 1 cut(s) 214
BmsI GCATC 1 cut(s) 410
BpiI GAAGAC 1 cut(s) 82
Bpu1102I GCTNAGC 1 cut(s) 359
Bpu14I TTCGAA 1 cut(s) 807
BpuEI CTTGAG 2 cut(s) 233, 935
BpuMI CCSGG 1 cut(s) 214
BsaAI YACGTR 3 cut(s) 531, 553, 565
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 5 cut(s) 663, 831, 846, 891, 929
BsaWI WCCGGW 2 cut(s) 280, 628
BsaXI ACNNNNNCTCC 2 cut(s) 644, 674
Bsc4I CCNNNNNNNGG 9 cut(s) 219, 374, 422, 609, 719, 761, 762, 781, 955
Bse118I RCCGGY 1 cut(s) 280
Bse3DI GCAATG 1 cut(s) 1016
BseDI CCNNGG 5 cut(s) 663, 831, 846, 891, 929
BseGI GGATG 2 cut(s) 13, 964
BseLI CCNNNNNNNGG 9 cut(s) 219, 374, 422, 609, 719, 761, 762, 781, 955
BseMI GCAATG 1 cut(s) 1016
BseMII CTCAG 2 cut(s) 350, 914
BseRI GAGGAG 1 cut(s) 880
BseXI GCAGC 3 cut(s) 429, 442, 450
BsgI GTGCAG 1 cut(s) 449
Bsh1236I CGCG 7 cut(s) 182, 437, 475, 518, 600, 665, 782
BshFI GGCC 3 cut(s) 404, 797, 830
BshNI GGYRCC 1 cut(s) 283
BshTI ACCGGT 1 cut(s) 280
BsiHKAI GWGCWC 2 cut(s) 249, 572
BsiHKCI CYCGRG 2 cut(s) 755, 916
BsiSI CCGG 3 cut(s) 213, 281, 629
BsiWI CGTACG 1 cut(s) 531
BslFI GGGAC 1 cut(s) 154
BslI CCNNNNNNNGG 9 cut(s) 219, 374, 422, 609, 719, 761, 762, 781, 955
BsmFI GGGAC 1 cut(s) 154
BsnI GGCC 3 cut(s) 404, 797, 830
BsoBI CYCGRG 2 cut(s) 755, 916
Bsp119I TTCGAA 1 cut(s) 807
Bsp1286I GDGCHC 3 cut(s) 249, 514, 572
Bsp143I GATC 2 cut(s) 3, 694
Bsp1720I GCTNAGC 1 cut(s) 359
BspANI GGCC 3 cut(s) 404, 797, 830
BspCNI CTCAG 2 cut(s) 351, 915
BspFNI CGCG 7 cut(s) 182, 437, 475, 518, 600, 665, 782
BspLI GGNNCC 4 cut(s) 224, 285, 513, 770
BspT104I TTCGAA 1 cut(s) 807
BspT107I GGYRCC 1 cut(s) 283
BsrDI GCAATG 1 cut(s) 1016
BsrFI RCCGGY 1 cut(s) 280
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 5 cut(s) 663, 831, 846, 891, 929
BssMI GATC 2 cut(s) 3, 694
BssNI GRCGYC 1 cut(s) 210
BssT1I CCWWGG 1 cut(s) 929
Bst4CI ACNGT 3 cut(s) 145, 266, 984
Bst6I CTCTTC 3 cut(s) 64, 317, 1124
BstACI GRCGYC 1 cut(s) 210
BstBAI YACGTR 3 cut(s) 531, 553, 565
BstBI TTCGAA 1 cut(s) 807
BstC8I GCNNGC 6 cut(s) 39, 227, 365, 449, 453, 710
BstDEI CTNAG 5 cut(s) 85, 359, 923, 936, 1077
BstDSI CCRYGG 3 cut(s) 663, 831, 846
BstF5I GGATG 2 cut(s) 13, 964
BstFNI CGCG 7 cut(s) 182, 437, 475, 518, 600, 665, 782
BstHHI GCGC 2 cut(s) 447, 602
BstKTI GATC 2 cut(s) 6, 697
BstMBI GATC 2 cut(s) 3, 694
BstMWI GCNNNNNNNGC 6 cut(s) 364, 429, 448, 472, 567, 699
BstNSI RCATGY 3 cut(s) 241, 451, 872
BstSCI CCNGG 1 cut(s) 212
BstUI CGCG 7 cut(s) 182, 437, 475, 518, 600, 665, 782
BstV1I GCAGC 3 cut(s) 429, 442, 450
BstV2I GAAGAC 1 cut(s) 82
BsuRI GGCC 3 cut(s) 404, 797, 830
BtgI CCRYGG 3 cut(s) 663, 831, 846
BtrI CACGTC 1 cut(s) 964
BtsCI GGATG 2 cut(s) 13, 964
BtsIMutI CAGTG 1 cut(s) 980
Cac8I GCNNGC 6 cut(s) 39, 227, 365, 449, 453, 710
CaiI CAGNNNCTG 1 cut(s) 982
CfoI GCGC 2 cut(s) 447, 602
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 2 cut(s) 795, 946
Cfr42I CCGCGG 1 cut(s) 666
CseI GACGC 5 cut(s) 218, 443, 464, 481, 788
Csp6I GTAC 5 cut(s) 256, 532, 872, 913, 985
CspAI ACCGGT 1 cut(s) 280
CviAII CATG 7 cut(s) 34, 105, 238, 448, 857, 869, 910
CviQI GTAC 5 cut(s) 256, 532, 872, 913, 985
DdeI CTNAG 5 cut(s) 85, 359, 923, 936, 1077
DpnI GATC 2 cut(s) 5, 696
DpnII GATC 2 cut(s) 3, 694
EaeI YGGCCR 2 cut(s) 402, 828
Eam1104I CTCTTC 3 cut(s) 64, 317, 1124
EarI CTCTTC 3 cut(s) 64, 317, 1124
EciI GGCGGA 1 cut(s) 800
Ecl136II GAGCTC 2 cut(s) 247, 570
Eco130I CCWWGG 1 cut(s) 929
Eco24I GRGCYC 3 cut(s) 249, 514, 572
Eco47I GGWCC 1 cut(s) 946
Eco53kI GAGCTC 2 cut(s) 247, 570
Eco57I CTGAAG 1 cut(s) 523
Eco88I CYCGRG 2 cut(s) 755, 916
EcoICRI GAGCTC 2 cut(s) 247, 570
EcoT14I CCWWGG 1 cut(s) 929
EcoT38I GRGCYC 3 cut(s) 249, 514, 572
ErhI CCWWGG 1 cut(s) 929
FaeI CATG 7 cut(s) 37, 108, 241, 451, 860, 872, 913
FalI AAGNNNNNCTT 4 cut(s) 242, 274, 347, 379
FaqI GGGAC 1 cut(s) 154
FatI CATG 7 cut(s) 33, 104, 237, 447, 856, 868, 909
FauI CCCGC 5 cut(s) 107, 121, 142, 430, 523
Fnu4HI GCNGC 6 cut(s) 180, 424, 443, 456, 464, 783
FokI GGATG 1 cut(s) 971
FriOI GRGCYC 3 cut(s) 249, 514, 572
Fsp4HI GCNGC 6 cut(s) 180, 424, 443, 456, 464, 783
FspBI CTAG 2 cut(s) 737, 930
FspI TGCGCA 1 cut(s) 446
GlaI GCGC 2 cut(s) 446, 601
GluI GCNGC 6 cut(s) 180, 424, 443, 456, 464, 783
HaeIII GGCC 3 cut(s) 404, 797, 830
HapII CCGG 3 cut(s) 213, 281, 629
HgaI GACGC 5 cut(s) 218, 443, 464, 481, 788
HhaI GCGC 2 cut(s) 447, 602
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 7 cut(s) 37, 108, 241, 451, 860, 872, 913
Hin6I GCGC 2 cut(s) 445, 600
HinP1I GCGC 2 cut(s) 445, 600
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HinfI GANTC 9 cut(s) 120, 329, 340, 386, 625, 809, 842, 919, 925
HpaII CCGG 3 cut(s) 213, 281, 629
HphI GGTGA 3 cut(s) 295, 400, 634
Hpy166II GTNNAC 3 cut(s) 634, 946, 1083
Hpy188I TCNGA 8 cut(s) 244, 339, 391, 577, 814, 924, 1027, 1127
Hpy188III TCNNGA 4 cut(s) 196, 250, 755, 952
Hpy8I GTNNAC 3 cut(s) 634, 946, 1083
Hpy99I CGWCG 7 cut(s) 437, 475, 480, 577, 707, 782, 803
HpyAV CCTTC 5 cut(s) 211, 547, 613, 817, 962
HpyCH4III ACNGT 3 cut(s) 145, 266, 984
HpyCH4IV ACGT 5 cut(s) 165, 530, 552, 564, 963
HpyCH4V TGCA 3 cut(s) 458, 466, 1021
HpyF10VI GCNNNNNNNGC 6 cut(s) 364, 429, 448, 472, 567, 699
HpyF3I CTNAG 5 cut(s) 85, 359, 923, 936, 1077
HpySE526I ACGT 5 cut(s) 165, 530, 552, 564, 963
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 7 cut(s) 37, 108, 241, 451, 860, 872, 913
HspAI GCGC 2 cut(s) 445, 600
KspI CCGCGG 1 cut(s) 666
Kzo9I GATC 2 cut(s) 3, 694
LmnI GCTCC 3 cut(s) 222, 244, 517
Lsp1109I GCAGC 3 cut(s) 429, 442, 450
LweI GCATC 1 cut(s) 410
MaeI CTAG 2 cut(s) 737, 930
MaeII ACGT 5 cut(s) 165, 530, 552, 564, 963
MaeIII GTNAC 4 cut(s) 50, 139, 370, 1048
MalI GATC 2 cut(s) 5, 696
MboI GATC 2 cut(s) 3, 694
MboII GAAGA 6 cut(s) 51, 70, 82, 304, 1111, 1114
MhlI GDGCHC 3 cut(s) 249, 514, 572
MluCI AATT 4 cut(s) 1000, 1030, 1054, 1086
MluI ACGCGT 1 cut(s) 473
MlyI GAGTC 5 cut(s) 334, 380, 851, 928, 934
MmeI TCCRAC 3 cut(s) 222, 555, 1005
MnlI CCTC 9 cut(s) 320, 421, 513, 577, 670, 759, 785, 901, 959
MroXI GAANNNNTTC 2 cut(s) 257, 972
MseI TTAA 1 cut(s) 149
MspA1I CMGCKG 2 cut(s) 229, 665
MspI CCGG 3 cut(s) 213, 281, 629
MspR9I CCNGG 1 cut(s) 214
MvnI CGCG 7 cut(s) 182, 437, 475, 518, 600, 665, 782
MwoI GCNNNNNNNGC 6 cut(s) 364, 429, 448, 472, 567, 699
NciI CCSGG 1 cut(s) 214
NdeII GATC 2 cut(s) 3, 694
NlaIII CATG 7 cut(s) 37, 108, 241, 451, 860, 872, 913
NlaIV GGNNCC 4 cut(s) 224, 285, 513, 770
NmeAIII GCCGAG 2 cut(s) 380, 441
NmuCI GTSAC 1 cut(s) 370
NsbI TGCGCA 1 cut(s) 446
NspI RCATGY 3 cut(s) 241, 451, 872
NspV TTCGAA 1 cut(s) 807
PaeI GCATGC 1 cut(s) 451
PcsI WCGNNNNNNNCGW 3 cut(s) 705, 818, 969
PdmI GAANNNNTTC 2 cut(s) 257, 972
PfeI GAWTC 4 cut(s) 120, 329, 625, 809
Pfl23II CGTACG 1 cut(s) 531
PinAI ACCGGT 1 cut(s) 280
PkrI GCNGC 6 cut(s) 181, 425, 444, 457, 465, 784
PleI GAGTC 5 cut(s) 334, 380, 850, 927, 933
PpsI GAGTC 5 cut(s) 334, 380, 850, 927, 933
Ppu21I YACGTR 3 cut(s) 531, 553, 565
Psp124BI GAGCTC 2 cut(s) 249, 572
PspLI CGTACG 1 cut(s) 531
PspN4I GGNNCC 4 cut(s) 224, 285, 513, 770
PspPI GGNCC 2 cut(s) 795, 946
PstNI CAGNNNCTG 1 cut(s) 982
PvuII CAGCTG 1 cut(s) 229
RsaI GTAC 5 cut(s) 257, 533, 873, 914, 986
RsaNI GTAC 5 cut(s) 256, 532, 872, 913, 985
SacI GAGCTC 2 cut(s) 249, 572
SacII CCGCGG 1 cut(s) 666
SaqAI TTAA 1 cut(s) 149
SatI GCNGC 6 cut(s) 180, 424, 443, 456, 464, 783
Sau3AI GATC 2 cut(s) 3, 694
Sau96I GGNCC 2 cut(s) 795, 946
ScaI AGTACT 1 cut(s) 257
SchI GAGTC 5 cut(s) 334, 380, 851, 928, 934
ScrFI CCNGG 1 cut(s) 214
SduI GDGCHC 3 cut(s) 249, 514, 572
SfaNI GCATC 1 cut(s) 410
Sfr303I CCGCGG 1 cut(s) 666
SfuI TTCGAA 1 cut(s) 807
SgrBI CCGCGG 1 cut(s) 666
SinI GGWCC 1 cut(s) 946
SmlI CTYRAG 2 cut(s) 248, 950
SmoI CTYRAG 2 cut(s) 248, 950
SphI GCATGC 1 cut(s) 451
Sse9I AATT 4 cut(s) 1000, 1030, 1054, 1086
SspI AATATT 1 cut(s) 591
SspMI CTAG 2 cut(s) 737, 930
SstI GAGCTC 2 cut(s) 249, 572
StyD4I CCNGG 1 cut(s) 212
StyI CCWWGG 1 cut(s) 929
TaaI ACNGT 3 cut(s) 145, 266, 984
TaiI ACGT 5 cut(s) 168, 533, 555, 567, 966
TaqI TCGA 7 cut(s) 315, 384, 717, 777, 801, 807, 821
TaqII GACCGA 1 cut(s) 883
TasI AATT 4 cut(s) 1000, 1030, 1054, 1086
TatI WGTACW 2 cut(s) 255, 871
TauI GCSGC 3 cut(s) 182, 426, 785
TfiI GAWTC 4 cut(s) 120, 329, 625, 809
Tru1I TTAA 1 cut(s) 149
Tru9I TTAA 1 cut(s) 149
TscAI CASTG 1 cut(s) 987
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 3 cut(s) 442, 455, 463
Tsp45I GTSAC 1 cut(s) 370
TspGWI ACGGA 2 cut(s) 58, 835
TspRI CASTG 1 cut(s) 987
VpaK11BI GGWCC 1 cut(s) 946
XapI RAATTY 1 cut(s) 1030
XceI RCATGY 3 cut(s) 241, 451, 872
XmaJI CCTAGG 1 cut(s) 929
XmnI GAANNNNTTC 2 cut(s) 257, 972
XspI CTAG 2 cut(s) 737, 930
ZrmI AGTACT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.