MD04G1063000.v1.1

Belongs to the peptidase M10A family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015037
Physical Location & Seq
Forward (+)
8325353 .. 8326444
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1063000.v1.1.491

Sequence Viewer

Length: 1092 bp
ATGTTCCCATTTTTCAGGTACAACTCTTTCTTCTTCTTCATATGCCTATGCTTCCTCTGTTTTTCCCCCCCTGCCTTTCCCGCCAGAATTTTACCGGACTCAGTAACTGTATTAACCGCCGCCTCCGACGTCCACAACGCCACATGGCACGACTTCTCAAGGTTCCTAGACGCCGGGAAAGGAAGCCTAGTTAGCGGCATGTCCGAGCTCAAGAACTACTTCCACCGTTTTGGCTACTTACCCAATTCCAACTTCACTGATTTTTTCGACACAGAATTTGAGTCCGCCTTGACTCTATACCAGTCCAAGCTGAGCCTGCCCGTCACAGGCAAGCTGGACACAGACACCATCTCGGCGATAATTTCACCGAGGTGTGGTGTCAGTGACATGTCGCAACATACGGCTCTGCATGCGACGCGTCACTTCGCCTACTTCTACGGCAAGCCGAGGTGGGCGCGCCCTTCCCCCGTATCGCTCACATACGCTTTCTCCCCCAATCACATGGTGAGTTACCTGAACCCATCAGATGTTCGAGACATTTTTCACCGCGCTTTTTCGCGGTGGTCGGCGGTGATCCCTGTGAACTTCACCGAGACCGACGACTACGCTTCCGCGGATATTAAAATAGGGTTTTACCGCGGCGACCACGGGGACGGGGAGCCGTTCGACGGGGTGTTGGGGGTGCTGGGGCATGCTTTTTCGCCCGAGAACGGGAGGTTCCACCTGGACGCGGCGGAATCATGGGCCATTGATTTCAAAACGGATAAGTCAAAGGTGGCTATTGATTTGGAATCGGTGGCGACTCATGAGATAGGGCATGTACTCGGGCTGGCTCACGCCTCGGTCAAGGAGGCCGTTATGTACCCGAGCCTGAGTCCTCGGACCAAGAAGGTGGACCTCAAGATTGATGACGTGGAAGGTGTCCAAGCTTTGTATGGGTCCAACCCTAATTTCAAATTTGGCTCATTGCAGAGTGAAAATTCTTACAACCATGCAGTTTCTTTGAAGTCAAGGTCTTCCTTTAGGTGGTCCATTTTGTTAACACTCACCACCACTTTGGTATTGCTCTTAGGCCTTAATTTGGCCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

364

Amino Acids

40.5

Weight (kDa)

6.3

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 67 - 120 4.1e-10 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 155 - 313 7.3e-51 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16640
fragaria_vesca FvH4_5g19070
malus_domestica MD04G1063000.v1.1 MD06G1057300.v1.1
prunus_persica Prupe.5G068200_v2.0.a1
pyrus_communis pycom04g05670
rosa_chinensis RchiOBHm_Chr7g0203521
rosa_laevigata RLG00000003523
rosa_multiflora Rmu_sc0022597.1_g000010
rosa_roxburghii Rroxscaffold_3G00253740
rosa_rugosa Rorug07G0076400 Rorug07G0076500
rosa_samantha Rh7AG205900 Rh7CG218700 Rh7DG213100
rosa_wichuraiana Rw7G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 132
AccII CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
AclWI GGATC 1 cut(s) 568
AcoI YGGCCR 1 cut(s) 1083
AcsI RAATTY 4 cut(s) 87, 275, 956, 979
AcyI GRCGYC 2 cut(s) 129, 171
AdeI CACNNNGTG 1 cut(s) 505
AfaI GTAC 3 cut(s) 20, 822, 863
AfiI CCNNNNNNNGG 8 cut(s) 326, 374, 668, 710, 711, 730, 1026, 1081
AflIII ACRYGT 2 cut(s) 387, 416
AgsI TTSAA 3 cut(s) 757, 955, 1006
AjiI CACGTC 1 cut(s) 913
AjnI CCWGG 1 cut(s) 723
AjuI GAANNNNNNNTTGG 4 cut(s) 236, 268, 935, 967
AleI CACNNNNGTG 1 cut(s) 370
AluBI AGCT 4 cut(s) 208, 310, 334, 929
AluI AGCT 4 cut(s) 208, 310, 334, 929
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 2 cut(s) 528, 587
AlwI GGATC 1 cut(s) 568
AlwNI CAGNNNCTG 1 cut(s) 107
Ama87I CYCGRG 3 cut(s) 704, 824, 865
AoxI GGCC 4 cut(s) 744, 852, 1072, 1083
ApoI RAATTY 4 cut(s) 87, 275, 956, 979
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
AscI GGCGCGCC 1 cut(s) 455
Asp700I GAANNNNTTC 1 cut(s) 218
AspLEI GCGC 3 cut(s) 457, 459, 551
AspS9I GGNCC 5 cut(s) 744, 882, 895, 939, 1029
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 6 cut(s) 357, 517, 536, 580, 583, 1039
AvaI CYCGRG 3 cut(s) 704, 824, 865
AvaII GGWCC 4 cut(s) 882, 895, 939, 1029
BalI TGGCCA 1 cut(s) 1085
BanII GRGCYC 1 cut(s) 210
BbsI GAAGAC 1 cut(s) 1008
Bbv12I GWGCWC 1 cut(s) 210
BccI CCATC 2 cut(s) 356, 529
BceAI ACGGC 4 cut(s) 417, 454, 646, 839
BciT130I CCWGG 1 cut(s) 725
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 2 cut(s) 528, 587
BfaI CTAG 2 cut(s) 167, 188
BisI GCNGC 4 cut(s) 120, 196, 640, 732
BlpI GCTNAGC 1 cut(s) 311
BlsI GCNGC 4 cut(s) 121, 197, 641, 733
Bme1390I CCNGG 2 cut(s) 175, 725
Bme18I GGWCC 4 cut(s) 882, 895, 939, 1029
BmeT110I CYCGRG 3 cut(s) 704, 824, 865
BmgBI CACGTC 1 cut(s) 913
BmgT120I GGNCC 5 cut(s) 744, 882, 895, 939, 1029
BmiI GGNNCC 4 cut(s) 164, 660, 719, 940
BmrFI CCNGG 2 cut(s) 175, 725
BpiI GAAGAC 1 cut(s) 1008
Bpu1102I GCTNAGC 1 cut(s) 311
BpuEI CTTGAG 3 cut(s) 142, 194, 884
BpuMI CCSGG 1 cut(s) 175
BsaHI GRCGYC 2 cut(s) 129, 171
BsaI GGTCTC 1 cut(s) 587
BsaJI CCNNGG 7 cut(s) 368, 446, 612, 637, 646, 840, 878
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bsc4I CCNNNNNNNGG 8 cut(s) 326, 374, 668, 710, 711, 730, 1026, 1081
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 965
BseBI CCWGG 1 cut(s) 725
BseDI CCNNGG 7 cut(s) 368, 446, 612, 637, 646, 840, 878
BseLI CCNNNNNNNGG 8 cut(s) 326, 374, 668, 710, 711, 730, 1026, 1081
BseMI GCAATG 1 cut(s) 965
BseMII CTCAG 3 cut(s) 114, 302, 863
BseNI ACTGG 1 cut(s) 301
BsePI GCGCGC 1 cut(s) 455
BseYI CCCAGC 1 cut(s) 685
Bsh1236I CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
BshFI GGCC 4 cut(s) 746, 854, 1074, 1085
BsiHKAI GWGCWC 1 cut(s) 210
BsiHKCI CYCGRG 3 cut(s) 704, 824, 865
BsiSI CCGG 2 cut(s) 95, 174
BslFI GGGAC 1 cut(s) 665
BslI CCNNNNNNNGG 8 cut(s) 326, 374, 668, 710, 711, 730, 1026, 1081
BsmAI GTCTC 2 cut(s) 528, 587
BsmFI GGGAC 1 cut(s) 665
BsnI GGCC 4 cut(s) 746, 854, 1074, 1085
Bso31I GGTCTC 1 cut(s) 587
BsoBI CYCGRG 3 cut(s) 704, 824, 865
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 1 cut(s) 573
Bsp1720I GCTNAGC 1 cut(s) 311
BspANI GGCC 4 cut(s) 746, 854, 1074, 1085
BspCNI CTCAG 3 cut(s) 113, 303, 864
BspFNI CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
BspHI TCATGA 1 cut(s) 805
BspLI GGNNCC 4 cut(s) 164, 660, 719, 940
BspPI GGATC 1 cut(s) 568
BspTNI GGTCTC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 965
BsrI ACTGG 1 cut(s) 301
BssECI CCNNGG 7 cut(s) 368, 446, 612, 637, 646, 840, 878
BssHII GCGCGC 1 cut(s) 455
BssMI GATC 1 cut(s) 573
BssNI GRCGYC 2 cut(s) 129, 171
Bst2UI CCWGG 1 cut(s) 725
Bst4CI ACNGT 2 cut(s) 109, 227
BstACI GRCGYC 2 cut(s) 129, 171
BstC8I GCNNGC 7 cut(s) 317, 332, 411, 443, 457, 693, 831
BstDEI CTNAG 4 cut(s) 100, 311, 872, 1069
BstDSI CCRYGG 3 cut(s) 612, 637, 646
BstFNI CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
BstHHI GCGC 3 cut(s) 457, 459, 551
BstKTI GATC 1 cut(s) 576
BstMAI GTCTC 2 cut(s) 528, 587
BstMBI GATC 1 cut(s) 573
BstMWI GCNNNNNNNGC 5 cut(s) 80, 192, 316, 410, 415
BstNI CCWGG 1 cut(s) 725
BstNSI RCATGY 5 cut(s) 202, 391, 413, 695, 821
BstSCI CCNGG 2 cut(s) 173, 723
BstUI CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
BstV2I GAAGAC 1 cut(s) 1008
BstXI CCANNNNNNTGG 4 cut(s) 230, 502, 892, 1057
BsuRI GGCC 4 cut(s) 746, 854, 1074, 1085
BtgI CCRYGG 3 cut(s) 612, 637, 646
BtrI CACGTC 1 cut(s) 913
BtsIMutI CAGTG 2 cut(s) 255, 388
Cac8I GCNNGC 7 cut(s) 317, 332, 411, 443, 457, 693, 831
CaiI CAGNNNCTG 1 cut(s) 107
CciI TCATGA 1 cut(s) 805
CfoI GCGC 3 cut(s) 457, 459, 551
Cfr13I GGNCC 5 cut(s) 744, 882, 895, 939, 1029
Cfr42I CCGCGG 2 cut(s) 615, 640
CseI GACGC 4 cut(s) 179, 407, 424, 737
Csp6I GTAC 3 cut(s) 19, 821, 862
CviQI GTAC 3 cut(s) 19, 821, 862
DdeI CTNAG 4 cut(s) 100, 311, 872, 1069
DpnI GATC 1 cut(s) 575
DpnII GATC 1 cut(s) 573
DraIII CACNNNGTG 1 cut(s) 505
EaeI YGGCCR 1 cut(s) 1083
EciI GGCGGA 2 cut(s) 274, 749
Ecl136II GAGCTC 1 cut(s) 208
Eco147I AGGCCT 1 cut(s) 1074
Eco24I GRGCYC 1 cut(s) 210
Eco31I GGTCTC 1 cut(s) 587
Eco47I GGWCC 4 cut(s) 882, 895, 939, 1029
Eco53kI GAGCTC 1 cut(s) 208
Eco88I CYCGRG 3 cut(s) 704, 824, 865
EcoICRI GAGCTC 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 723
EcoT38I GRGCYC 1 cut(s) 210
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 1 cut(s) 665
FauI CCCGC 1 cut(s) 88
FauNDI CATATG 1 cut(s) 41
Fnu4HI GCNGC 4 cut(s) 120, 196, 640, 732
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 4 cut(s) 120, 196, 640, 732
FspBI CTAG 2 cut(s) 167, 188
GlaI GCGC 3 cut(s) 456, 458, 550
GluI GCNGC 4 cut(s) 120, 196, 640, 732
GsaI CCCAGC 1 cut(s) 689
HaeIII GGCC 4 cut(s) 746, 854, 1074, 1085
HapII CCGG 2 cut(s) 95, 174
HgaI GACGC 4 cut(s) 179, 407, 424, 737
HhaI GCGC 3 cut(s) 457, 459, 551
Hin1I GRCGYC 2 cut(s) 129, 171
Hin6I GCGC 3 cut(s) 455, 457, 549
HinP1I GCGC 3 cut(s) 455, 457, 549
HincII GTYRAC 1 cut(s) 1041
HindII GTYRAC 1 cut(s) 1041
HindIII AAGCTT 1 cut(s) 927
HinfI GANTC 7 cut(s) 98, 281, 292, 737, 791, 802, 874
HpaI GTTAAC 1 cut(s) 1041
HpaII CCGG 2 cut(s) 95, 174
HphI GGTGA 6 cut(s) 357, 517, 536, 580, 583, 1039
Hpy166II GTNNAC 4 cut(s) 133, 583, 895, 1041
Hpy188I TCNGA 4 cut(s) 127, 205, 526, 882
Hpy188III TCNNGA 4 cut(s) 211, 533, 806, 901
Hpy8I GTNNAC 4 cut(s) 133, 583, 895, 1041
Hpy99I CGWCG 4 cut(s) 131, 418, 602, 671
HpyAV CCTTC 3 cut(s) 471, 883, 911
HpyCH4III ACNGT 2 cut(s) 109, 227
HpyCH4IV ACGT 2 cut(s) 129, 912
HpyCH4V TGCA 3 cut(s) 409, 970, 995
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 192, 316, 410, 415
HpyF3I CTNAG 4 cut(s) 100, 311, 872, 1069
HpySE526I ACGT 2 cut(s) 129, 912
Hsp92I GRCGYC 2 cut(s) 129, 171
HspAI GCGC 3 cut(s) 455, 457, 549
KspAI GTTAAC 1 cut(s) 1041
KspI CCGCGG 2 cut(s) 615, 640
Kzo9I GATC 1 cut(s) 573
LmnI GCTCC 1 cut(s) 658
MaeI CTAG 2 cut(s) 167, 188
MaeII ACGT 2 cut(s) 129, 912
MaeIII GTNAC 5 cut(s) 103, 322, 383, 419, 509
MalI GATC 1 cut(s) 575
MboI GATC 1 cut(s) 573
MboII GAAGA 4 cut(s) 22, 25, 28, 1008
MhlI GDGCHC 1 cut(s) 210
MlsI TGGCCA 1 cut(s) 1085
MluCI AATT 8 cut(s) 87, 244, 275, 360, 949, 956, 979, 1078
MluI ACGCGT 1 cut(s) 416
MluNI TGGCCA 1 cut(s) 1085
MlyI GAGTC 5 cut(s) 92, 286, 290, 796, 883
MmeI TCCRAC 3 cut(s) 150, 273, 966
MnlI CCTC 9 cut(s) 65, 133, 363, 441, 708, 844, 850, 888, 908
Mox20I TGGCCA 1 cut(s) 1085
MroXI GAANNNNTTC 1 cut(s) 218
MscI TGGCCA 1 cut(s) 1085
MseI TTAA 4 cut(s) 113, 621, 1040, 1077
MslI CAYNNNNRTG 1 cut(s) 370
Msp20I TGGCCA 1 cut(s) 1085
MspA1I CMGCKG 2 cut(s) 614, 639
MspI CCGG 2 cut(s) 95, 174
MspR9I CCNGG 2 cut(s) 175, 725
MvaI CCWGG 1 cut(s) 725
MvnI CGCG 7 cut(s) 418, 457, 549, 559, 614, 639, 731
MwoI GCNNNNNNNGC 5 cut(s) 80, 192, 316, 410, 415
NciI CCSGG 1 cut(s) 175
NdeI CATATG 1 cut(s) 41
NdeII GATC 1 cut(s) 573
NlaIV GGNNCC 4 cut(s) 164, 660, 719, 940
NmeAIII GCCGAG 2 cut(s) 332, 471
NmuCI GTSAC 3 cut(s) 322, 383, 419
NspI RCATGY 5 cut(s) 202, 391, 413, 695, 821
OliI CACNNNNGTG 1 cut(s) 370
PaeI GCATGC 2 cut(s) 413, 695
PagI TCATGA 1 cut(s) 805
PalAI GGCGCGCC 1 cut(s) 455
PauI GCGCGC 1 cut(s) 455
PceI AGGCCT 1 cut(s) 1074
PciI ACATGT 1 cut(s) 387
PdmI GAANNNNTTC 1 cut(s) 218
PfeI GAWTC 2 cut(s) 737, 791
PkrI GCNGC 4 cut(s) 121, 197, 641, 733
PleI GAGTC 5 cut(s) 92, 286, 289, 796, 882
PpsI GAGTC 5 cut(s) 92, 286, 289, 796, 882
PscI ACATGT 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 723
PspFI CCCAGC 1 cut(s) 685
PspGI CCWGG 1 cut(s) 723
PspN4I GGNNCC 4 cut(s) 164, 660, 719, 940
PspPI GGNCC 5 cut(s) 744, 882, 895, 939, 1029
PstNI CAGNNNCTG 1 cut(s) 107
PteI GCGCGC 1 cut(s) 455
RsaI GTAC 3 cut(s) 20, 822, 863
RsaNI GTAC 3 cut(s) 19, 821, 862
RseI CAYNNNNRTG 1 cut(s) 370
SacI GAGCTC 1 cut(s) 210
SacII CCGCGG 2 cut(s) 615, 640
SaqAI TTAA 4 cut(s) 113, 621, 1040, 1077
SatI GCNGC 4 cut(s) 120, 196, 640, 732
Sau3AI GATC 1 cut(s) 573
Sau96I GGNCC 5 cut(s) 744, 882, 895, 939, 1029
SchI GAGTC 5 cut(s) 92, 286, 290, 796, 883
ScrFI CCNGG 2 cut(s) 175, 725
SduI GDGCHC 1 cut(s) 210
Sfr303I CCGCGG 2 cut(s) 615, 640
SgrBI CCGCGG 2 cut(s) 615, 640
SgsI GGCGCGCC 1 cut(s) 455
SinI GGWCC 4 cut(s) 882, 895, 939, 1029
SmiMI CAYNNNNRTG 1 cut(s) 370
SmlI CTYRAG 3 cut(s) 157, 209, 899
SmoI CTYRAG 3 cut(s) 157, 209, 899
SphI GCATGC 2 cut(s) 413, 695
Sse9I AATT 8 cut(s) 87, 244, 275, 360, 949, 956, 979, 1078
SseBI AGGCCT 1 cut(s) 1074
SspMI CTAG 2 cut(s) 167, 188
SstI GAGCTC 1 cut(s) 210
StuI AGGCCT 1 cut(s) 1074
StyD4I CCNGG 2 cut(s) 173, 723
TaaI ACNGT 2 cut(s) 109, 227
TaiI ACGT 2 cut(s) 132, 915
TaqI TCGA 3 cut(s) 267, 532, 666
TaqII GACCGA 2 cut(s) 611, 832
TasI AATT 8 cut(s) 87, 244, 275, 360, 949, 956, 979, 1078
TatI WGTACW 1 cut(s) 820
TauI GCSGC 4 cut(s) 122, 198, 642, 734
TfiI GAWTC 2 cut(s) 737, 791
Tru1I TTAA 4 cut(s) 113, 621, 1040, 1077
Tru9I TTAA 4 cut(s) 113, 621, 1040, 1077
TscAI CASTG 2 cut(s) 262, 388
TseFI GTSAC 3 cut(s) 322, 383, 419
Tsp45I GTSAC 3 cut(s) 322, 383, 419
TspDTI ATGAA 1 cut(s) 28
TspGWI ACGGA 1 cut(s) 776
TspRI CASTG 2 cut(s) 262, 388
VpaK11BI GGWCC 4 cut(s) 882, 895, 939, 1029
XapI RAATTY 4 cut(s) 87, 275, 956, 979
XceI RCATGY 5 cut(s) 202, 391, 413, 695, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 932
XmnI GAANNNNTTC 1 cut(s) 218
XspI CTAG 2 cut(s) 167, 188
ZraI GACGTC 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.