AT4G16660
HSP70 Family

heat shock protein 70

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007799
Physical Location & Seq
Forward (+)
9376699 .. 9381507
4809 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G16660.2

Sequence Viewer

Length: 2604 bp
ATGGGGAAGATATTTAGCTGGTTAGTGGTGTTATTGTCATTGATCTCATTAGTCCCTGTTCCATCGGAATCGGCTGTTTTGAGTGTAGATCTAGGTTCAGAATGGGTTAAAGTTGCCGTAGTTAATCTGAAACGAGGACAGAGTCCAATCTCTGTAGCCATTAATGAGATGTCCAAGAGGAAATCCCCAGCTTTAGTAGCATTTCAATCTGGAGACCGGTTACTAGGCGAGGAGGCTGCGGGAATAACGGCTCGTTATCCGAATAAAGTTTATTCACAACTGAGGGATATGGTGGGAAAACCTTTCAAACATGTTAAGGATTTCATTGATTCTGTCTACTTGCCTTTTGATATTGTAGAAGATTCTAGAGGTGCAGTTGGTATTAAGATCGATGACGGTTCGACTGTTTACTCAGTGGAGGAGTTGTTAGCTATGATCTTGGGTTATGCCTCAAATCTAGCTGAGTTCCATGCTAAAATCCCAGTGAAGGATATGGTTGTTTCAGTTCCACCTTACTTTGGACAGGCTGAGAGACGGGGTTTGATCCAGGCTTCTCAGTTGGCTGGGGTTAATGTCCTCTCCCTGGTTAATGAGCATTCCGGTGCTGCTTTGCAATATGGGATTGATAAGGATTTTGCTAATGGGTCGAGACATGTCATATTTTATGACATGGGTTCAAGTAGTACTTACGCAGCCCTTGTCTATTACTCAGCTTACAGTGAGAAGGAATATGGCAAGACTGTTTCTGTCAACCAATTTCAGGTCAAGGATGTTAGATGGGACTTGGGACTTGGAGGACAGAGTATGGAGATGCGCTTGGTAGAGCACTTTGCAGATGAGTTTAATAAACAGCTCGGTAATGGAGTTGATGTGAGGAAGTTCCCCAAAGCAATGGCTAAATTGAAAAAACAAGTCAAGCGTACAAAGGAAATTTTGAGTGCAAACACTGCGGCTCCAATATCTGTGGAATCTCTTCATGATGATCGTGACTTCAGGAGCACAATTACCCGTGAGAAGTTTGAGGAACTGTGTAAAGATCTGTGGGAGAGATCTCTTACACCTTTAAAAGATGTGCTCAAGCATTCAGGTTTGAAGATTGATGATATATCTGCAGTGGAACTAATAGGAGGAGCTACTAGAGTTCCCAAACTACAGAGCACAATCCAGGAATTTATTGGGAAACAACAGTTAGACAAACATCTAGACGCTGATGAGGCTATTGTCCTCGGCTCAGCACTACATGCTGCTAACTTGAGCGATGGAATTAAATTGAAACGTAGGCTAGGTATAGTTGATGGTTCCCCCTATGGTTTTCTGGTTGAGTTGGAAGGCCCTAATGTTAAGAAAGATGAGAGCACAAAGCAGCAACTCGTACCACGGATGAAAAAGCTACCCAGCAAGATGTTCAGATCCTTTGTCCTTGACAAAGATTTTGACGTGTCACTTGCTTATGAGTCTGAGGGTATTCTACCACCAGGAACCACCTCCCCTGTGTTTGCACAGTATTCTGTCTCTGGTTTGGCGGATGCATCTGAGAAATATTCTTCTCGGAATCTATCAGCACCTATCAAGGCAAATCTGCATTTCTCTCTAAGTAGAAGTGGGATTCTCAGTCTAGATCGAGGAGATGCTGTAATTGAAATCACAGAATGGGTAGACGTTCCTAAGAAGAACGTGACTATTGATAGTAACACAACTACATCAACAGGCAATGCCACTGACGAGAATTCACAAGAAAATAAAGAAGACCTGCAAACCGATGCTGAAAACAGCACTGCTTCAAATACAACAGCAGAAGAGCCAGCTGTTGCTTCTCTGGGCACAGAAAAAAAGCTGAAGAAGCGGACATTCAGAATACCTCTGAAGGTAGTTGAGAAAACTGTTGGACCTGGAGCACCATTTTCGAAAGAGTCTCTTGCTGAAGCTAAGATAAAATTAGAAGCCTTGGACAAGAAAGATAGGGAAAGAAGAAGAACGGCTGAGTTAAAAAACAACCTTGAATCTTATATATATGCTACCAAAGAGAAGCTGGAAACACCCGAGTTTGAAAAGATATCCACCCAAGAAGAGCGCAAGGCGTTTGTTGAAAAGCTTGATGAGGTGCAAGATTGGCTTTACATGGATGGAGAAGATGCTAATGCCACAGAGTTTGAGAAGCGGCTTGACTCACTTAAAGCCATCGGCAGTCCCATATCTTTCCGATCAGAGGAGCTTACAGCACGACCAGTAGCAATTGAATATGCTAGGAAATACCTAACTGAACTCAAAGAGATCATAAAAGAATGGGAGACGAACAAAACTTGGCTTCCAAAAGAAAAAATCGACGAGGTCTCAAAGGAAGCAGAGAAAGTAAAAAGCTGGTTAGATAAGAATGTGGCTGAGCAAGAAAAGACTTCTCTGTGGAGCAAACCGGTGTTCACGTCCACTGAAGTGTACGCTAAAGTATTTACTCTACAAGACAAGGTCACAAAGGTGAATAAGATCCCAAAGCCAAAGCCAAAGATAGAGAAAGTAACCAAGACGGAGAACACAACAAAGGAGGAGGAACAATCAAAATCCTCTGATGAAGCTGCCAAAGAAGAAGAAAGTCACGACGAGCTTTGA

Protein Analysis

867

Amino Acids

96.72

Weight (kDa)

5.86

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 51 - 755 2.6e-95 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1758
AccI GTMKAC 2 cut(s) 336, 1656
AciI CCGC 5 cut(s) 239, 950, 1523, 1843, 2158
AclWI GGATC 3 cut(s) 538, 1404, 2476
AcsI RAATTY 3 cut(s) 930, 1169, 1726
AcuI CTGAAG 5 cut(s) 976, 1856, 1883, 1941, 2448
AfaI GTAC 4 cut(s) 685, 922, 1374, 2435
AfiI CCNNNNNNNGG 5 cut(s) 487, 518, 583, 760, 2206
AflIII ACRYGT 3 cut(s) 310, 652, 1437
AgeI ACCGGT 2 cut(s) 216, 2410
AjiI CACGTC 2 cut(s) 1438, 2421
AjnI CCWGG 5 cut(s) 546, 582, 1164, 1474, 1888
AleI CACNNNNGTG 2 cut(s) 2429, 2471
Alw21I GWGCWC 6 cut(s) 828, 1001, 1077, 1160, 1358, 1897
Alw26I GTCTC 7 cut(s) 207, 526, 643, 1516, 1917, 2282, 2335
AlwI GGATC 3 cut(s) 538, 1404, 2476
Ama87I CYCGRG 1 cut(s) 2039
AoxI GGCC 1 cut(s) 1330
ApeKI GCWGC 6 cut(s) 236, 605, 692, 1244, 1363, 2570
ApoI RAATTY 3 cut(s) 930, 1169, 1726
ArsI GACNNNNNNTTYG 2 cut(s) 1415, 1447
AseI ATTAAT 1 cut(s) 162
AsiGI ACCGGT 2 cut(s) 216, 2410
Asp700I GAANNNNTTC 1 cut(s) 972
AspLEI GCGC 2 cut(s) 816, 2073
AspS9I GGNCC 2 cut(s) 1331, 1886
AsuHPI GGTGA 1 cut(s) 2485
AsuII TTCGAA 1 cut(s) 1904
AvaI CYCGRG 1 cut(s) 2039
AvaII GGWCC 1 cut(s) 1886
BaeGI GKGCMC 1 cut(s) 1823
BbsI GAAGAC 1 cut(s) 1752
Bbv12I GWGCWC 6 cut(s) 828, 1001, 1077, 1160, 1358, 1897
BbvI GCAGC 6 cut(s) 223, 592, 704, 1231, 1375, 2557
BccI CCATC 6 cut(s) 70, 771, 1253, 1289, 2117, 2186
BceAI ACGGC 3 cut(s) 101, 264, 1992
BcgI CGANNNNNNTGC 4 cut(s) 218, 252, 1884, 1918
BciT130I CCWGG 5 cut(s) 548, 584, 1166, 1476, 1890
BcoDI GTCTC 7 cut(s) 207, 526, 643, 1516, 1917, 2282, 2335
BfaI CTAG 9 cut(s) 92, 224, 366, 458, 1137, 1202, 1283, 1616, 2244
BfmI CTRYAG 3 cut(s) 153, 1110, 1151
BfuAI ACCTGC 1 cut(s) 1758
BglII AGATCT 3 cut(s) 88, 1036, 1049
BisI GCNGC 8 cut(s) 237, 606, 693, 951, 1245, 1364, 2159, 2571
BlpI GCTNAGC 2 cut(s) 1231, 2379
BlsI GCNGC 8 cut(s) 238, 607, 694, 952, 1246, 1365, 2160, 2572
BmcAI AGTACT 1 cut(s) 685
Bme1390I CCNGG 5 cut(s) 548, 584, 1166, 1476, 1890
Bme18I GGWCC 1 cut(s) 1886
BmeT110I CYCGRG 1 cut(s) 2039
BmgBI CACGTC 2 cut(s) 1438, 2421
BmgT120I GGNCC 2 cut(s) 1331, 1886
BmiI GGNNCC 3 cut(s) 954, 1300, 1480
BmrFI CCNGG 5 cut(s) 548, 584, 1166, 1476, 1890
BmrI ACTGGG 1 cut(s) 476
BmsI GCATC 6 cut(s) 801, 1516, 1538, 1618, 1750, 2122
BmuI ACTGGG 1 cut(s) 476
BpiI GAAGAC 1 cut(s) 1752
BpmI CTGGAG 2 cut(s) 231, 1911
Bpu1102I GCTNAGC 2 cut(s) 1231, 2379
Bpu14I TTCGAA 1 cut(s) 1904
BpuEI CTTGAG 2 cut(s) 1061, 1273
Bsa29I ATCGAT 1 cut(s) 390
BsaI GGTCTC 2 cut(s) 207, 2335
BsaJI CCNNGG 4 cut(s) 582, 1225, 1376, 1944
BsaWI WCCGGW 3 cut(s) 216, 599, 2410
BsaXI ACNNNNNCTCC 8 cut(s) 410, 440, 855, 885, 937, 967, 1617, 1647
Bsc4I CCNNNNNNNGG 5 cut(s) 487, 518, 583, 760, 2206
Bse118I RCCGGY 2 cut(s) 216, 2410
Bse1I ACTGG 2 cut(s) 482, 2225
Bse3DI GCAATG 2 cut(s) 897, 1717
BseBI CCWGG 5 cut(s) 548, 584, 1166, 1476, 1890
BseCI ATCGAT 1 cut(s) 390
BseDI CCNNGG 4 cut(s) 582, 1225, 1376, 1944
BseGI GGATG 4 cut(s) 775, 1386, 1531, 2128
BseLI CCNNNNNNNGG 5 cut(s) 487, 518, 583, 760, 2206
BseMI GCAATG 2 cut(s) 897, 1717
BseNI ACTGG 2 cut(s) 482, 2225
BseRI GAGGAG 6 cut(s) 245, 434, 1143, 1638, 2222, 2555
BseSI GKGCMC 1 cut(s) 1823
BseXI GCAGC 6 cut(s) 223, 592, 704, 1231, 1375, 2557
BseYI CCCAGC 3 cut(s) 187, 563, 1394
BsgI GTGCAG 1 cut(s) 393
BshFI GGCC 1 cut(s) 1332
BshTI ACCGGT 2 cut(s) 216, 2410
BshVI ATCGAT 1 cut(s) 390
BsiHKAI GWGCWC 6 cut(s) 828, 1001, 1077, 1160, 1358, 1897
BsiHKCI CYCGRG 1 cut(s) 2039
BsiSI CCGG 3 cut(s) 217, 600, 2411
BslFI GGGAC 4 cut(s) 38, 794, 801, 2172
BslI CCNNNNNNNGG 5 cut(s) 487, 518, 583, 760, 2206
BsmAI GTCTC 7 cut(s) 207, 526, 643, 1516, 1917, 2282, 2335
BsmBI CGTCTC 2 cut(s) 526, 2282
BsmFI GGGAC 4 cut(s) 38, 794, 801, 2172
BsmI GAATGC 2 cut(s) 595, 1081
BsnI GGCC 1 cut(s) 1332
Bso31I GGTCTC 2 cut(s) 207, 2335
BsoBI CYCGRG 1 cut(s) 2039
Bsp119I TTCGAA 1 cut(s) 1904
Bsp1286I GDGCHC 7 cut(s) 828, 1001, 1077, 1160, 1358, 1823, 1897
Bsp1720I GCTNAGC 2 cut(s) 1231, 2379
BspACI CCGC 5 cut(s) 239, 950, 1523, 1843, 2158
BspANI GGCC 1 cut(s) 1332
BspDI ATCGAT 1 cut(s) 390
BspHI TCATGA 1 cut(s) 976
BspLI GGNNCC 3 cut(s) 954, 1300, 1480
BspMAI CTGCAG 1 cut(s) 1114
BspMI ACCTGC 1 cut(s) 1758
BspPI GGATC 3 cut(s) 538, 1404, 2476
BspQI GCTCTTC 2 cut(s) 1791, 2061
BspT104I TTCGAA 1 cut(s) 1904
BspTNI GGTCTC 2 cut(s) 207, 2335
BsrDI GCAATG 2 cut(s) 897, 1717
BsrFI RCCGGY 2 cut(s) 216, 2410
BsrI ACTGG 2 cut(s) 482, 2225
BssAI RCCGGY 2 cut(s) 216, 2410
BssECI CCNNGG 4 cut(s) 582, 1225, 1376, 1944
BssT1I CCWWGG 1 cut(s) 1944
Bst2UI CCWGG 5 cut(s) 548, 584, 1166, 1476, 1890
Bst4CI ACNGT 8 cut(s) 398, 406, 719, 742, 1029, 1188, 1503, 1882
Bst6I CTCTTC 3 cut(s) 978, 1791, 2061
BstAPI GCANNNNNTGC 2 cut(s) 947, 1241
BstBI TTCGAA 1 cut(s) 1904
BstC8I GCNNGC 1 cut(s) 1803
BstDSI CCRYGG 1 cut(s) 1376
BstF5I GGATG 4 cut(s) 775, 1386, 1531, 2128
BstHHI GCGC 2 cut(s) 816, 2073
BstMAI GTCTC 7 cut(s) 207, 526, 643, 1516, 1917, 2282, 2335
BstMWI GCNNNNNNNGC 6 cut(s) 197, 947, 1214, 1241, 1840, 2110
BstNI CCWGG 5 cut(s) 548, 584, 1166, 1476, 1890
BstNSI RCATGY 3 cut(s) 314, 656, 1244
BstSCI CCNGG 5 cut(s) 546, 582, 1164, 1474, 1888
BstSFI CTRYAG 3 cut(s) 153, 1110, 1151
BstSLI GKGCMC 1 cut(s) 1823
BstV1I GCAGC 6 cut(s) 223, 592, 704, 1231, 1375, 2557
BstV2I GAAGAC 1 cut(s) 1752
BstX2I RGATCY 5 cut(s) 88, 1036, 1049, 1409, 2481
BstXI CCANNNNNNTGG 1 cut(s) 892
BstYI RGATCY 5 cut(s) 88, 1036, 1049, 1409, 2481
Bsu15I ATCGAT 1 cut(s) 390
BsuRI GGCC 1 cut(s) 1332
BsuTUI ATCGAT 1 cut(s) 390
BtgI CCRYGG 1 cut(s) 1376
BtgZI GCGATG 1 cut(s) 1272
BtrI CACGTC 2 cut(s) 1438, 2421
BtsCI GGATG 4 cut(s) 775, 1386, 1531, 2128
BtsI GCAGTG 3 cut(s) 945, 1119, 1773
BtsIMutI CAGTG 8 cut(s) 420, 489, 724, 945, 1119, 1716, 1773, 2424
BveI ACCTGC 1 cut(s) 1758
Cac8I GCNNGC 1 cut(s) 1803
CciI TCATGA 1 cut(s) 976
CfoI GCGC 2 cut(s) 816, 2073
Cfr10I RCCGGY 2 cut(s) 216, 2410
Cfr13I GGNCC 2 cut(s) 1331, 1886
ClaI ATCGAT 1 cut(s) 390
CseI GACGC 1 cut(s) 1214
Csp6I GTAC 4 cut(s) 684, 921, 1373, 2434
CspAI ACCGGT 2 cut(s) 216, 2410
CspCI CAANNNNNGTGG 4 cut(s) 945, 980, 2131, 2166
CviAII CATG 7 cut(s) 311, 470, 653, 670, 977, 1241, 2119
CviQI GTAC 4 cut(s) 684, 921, 1373, 2434
DraI TTTAAA 1 cut(s) 1065
Eam1104I CTCTTC 3 cut(s) 978, 1791, 2061
EarI CTCTTC 3 cut(s) 978, 1791, 2061
EciI GGCGGA 1 cut(s) 1538
Eco130I CCWWGG 1 cut(s) 1944
Eco31I GGTCTC 2 cut(s) 207, 2335
Eco32I GATATC 1 cut(s) 2055
Eco47I GGWCC 1 cut(s) 1886
Eco57I CTGAAG 5 cut(s) 976, 1856, 1883, 1941, 2448
Eco88I CYCGRG 1 cut(s) 2039
EcoO109I RGGNCCY 1 cut(s) 1331
EcoRI GAATTC 1 cut(s) 1726
EcoRII CCWGG 5 cut(s) 546, 582, 1164, 1474, 1888
EcoRV GATATC 1 cut(s) 2055
EcoT14I CCWWGG 1 cut(s) 1944
EcoT22I ATGCAT 1 cut(s) 1531
ErhI CCWWGG 1 cut(s) 1944
Esp3I CGTCTC 2 cut(s) 526, 2282
FaeI CATG 7 cut(s) 314, 473, 656, 673, 980, 1244, 2122
FalI AAGNNNNNCTT 6 cut(s) 670, 702, 1899, 1931, 2097, 2129
FaqI GGGAC 4 cut(s) 38, 794, 801, 2172
FatI CATG 7 cut(s) 310, 469, 652, 669, 976, 1240, 2118
FauI CCCGC 1 cut(s) 232
FblI GTMKAC 2 cut(s) 336, 1656
Fnu4HI GCNGC 8 cut(s) 237, 606, 693, 951, 1245, 1364, 2159, 2571
FokI GGATG 4 cut(s) 782, 1393, 1538, 2135
Fsp4HI GCNGC 8 cut(s) 237, 606, 693, 951, 1245, 1364, 2159, 2571
FspBI CTAG 9 cut(s) 92, 224, 366, 458, 1137, 1202, 1283, 1616, 2244
GlaI GCGC 2 cut(s) 815, 2072
GluI GCNGC 8 cut(s) 237, 606, 693, 951, 1245, 1364, 2159, 2571
GsaI CCCAGC 3 cut(s) 191, 567, 1398
GsuI CTGGAG 2 cut(s) 231, 1911
HaeIII GGCC 1 cut(s) 1332
HapII CCGG 3 cut(s) 217, 600, 2411
HgaI GACGC 1 cut(s) 1214
HhaI GCGC 2 cut(s) 816, 2073
Hin1II CATG 7 cut(s) 314, 473, 656, 673, 980, 1244, 2122
Hin6I GCGC 2 cut(s) 814, 2071
HinP1I GCGC 2 cut(s) 814, 2071
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HindIII AAGCTT 1 cut(s) 2090
HpaII CCGG 3 cut(s) 217, 600, 2411
HphI GGTGA 1 cut(s) 2485
Hpy166II GTNNAC 7 cut(s) 337, 409, 751, 1657, 2418, 2424, 2434
Hpy188III TCNNGA 9 cut(s) 210, 366, 648, 977, 986, 994, 1202, 1616, 2591
Hpy8I GTNNAC 7 cut(s) 337, 409, 751, 1657, 2418, 2424, 2434
Hpy99I CGWCG 2 cut(s) 2327, 2597
HpyAV CCTTC 4 cut(s) 481, 718, 1322, 1858
HpyCH4III ACNGT 8 cut(s) 398, 406, 719, 742, 1029, 1188, 1503, 1882
HpyCH4IV ACGT 5 cut(s) 1276, 1437, 1659, 1674, 2420
HpyF10VI GCNNNNNNNGC 6 cut(s) 197, 947, 1214, 1241, 1840, 2110
HpySE526I ACGT 5 cut(s) 1276, 1437, 1659, 1674, 2420
Hsp92II CATG 7 cut(s) 314, 473, 656, 673, 980, 1244, 2122
HspAI GCGC 2 cut(s) 814, 2071
LguI GCTCTTC 2 cut(s) 1791, 2061
LmnI GCTCC 6 cut(s) 958, 996, 1130, 1892, 2209, 2403
Lsp1109I GCAGC 6 cut(s) 223, 592, 704, 1231, 1375, 2557
LweI GCATC 6 cut(s) 801, 1516, 1538, 1618, 1750, 2122
MaeI CTAG 9 cut(s) 92, 224, 366, 458, 1137, 1202, 1283, 1616, 2244
MaeII ACGT 5 cut(s) 1276, 1437, 1659, 1674, 2420
MaeIII GTNAC 8 cut(s) 219, 986, 1440, 1675, 1688, 2464, 2512, 2588
MfeI CAATTG 1 cut(s) 2232
MflI RGATCY 5 cut(s) 88, 1036, 1049, 1409, 2481
MhlI GDGCHC 7 cut(s) 828, 1001, 1077, 1160, 1358, 1823, 1897
MlyI GAGTC 4 cut(s) 151, 1463, 1919, 2159
MmeI TCCRAC 2 cut(s) 1305, 1864
Mph1103I ATGCAT 1 cut(s) 1531
MroXI GAANNNNTTC 1 cut(s) 972
MslI CAYNNNNRTG 3 cut(s) 600, 2429, 2471
MspA1I CMGCKG 1 cut(s) 1805
MspI CCGG 3 cut(s) 217, 600, 2411
MspR9I CCNGG 5 cut(s) 548, 584, 1166, 1476, 1890
MunI CAATTG 1 cut(s) 2232
Mva1269I GAATGC 2 cut(s) 595, 1081
MvaI CCWGG 5 cut(s) 548, 584, 1166, 1476, 1890
MwoI GCNNNNNNNGC 6 cut(s) 197, 947, 1214, 1241, 1840, 2110
NlaIII CATG 7 cut(s) 314, 473, 656, 673, 980, 1244, 2122
NlaIV GGNNCC 3 cut(s) 954, 1300, 1480
NmeAIII GCCGAG 1 cut(s) 1206
NmuCI GTSAC 5 cut(s) 986, 1440, 1675, 2464, 2588
NsiI ATGCAT 1 cut(s) 1531
NspI RCATGY 3 cut(s) 314, 656, 1244
NspV TTCGAA 1 cut(s) 1904
OliI CACNNNNGTG 2 cut(s) 2429, 2471
PagI TCATGA 1 cut(s) 976
PciI ACATGT 2 cut(s) 310, 652
PciSI GCTCTTC 2 cut(s) 1791, 2061
PctI GAATGC 2 cut(s) 595, 1081
PdmI GAANNNNTTC 1 cut(s) 972
PfeI GAWTC 7 cut(s) 68, 329, 362, 968, 1552, 1606, 2000
PflFI GACNNNGTC 3 cut(s) 141, 2327, 2462
PfoI TCCNGGA 1 cut(s) 1164
PinAI ACCGGT 2 cut(s) 216, 2410
PkrI GCNGC 8 cut(s) 238, 607, 694, 952, 1246, 1365, 2160, 2572
PleI GAGTC 4 cut(s) 150, 1462, 1918, 2159
PpsI GAGTC 4 cut(s) 150, 1462, 1918, 2159
PscI ACATGT 2 cut(s) 310, 652
PshBI ATTAAT 1 cut(s) 162
Psp6I CCWGG 5 cut(s) 546, 582, 1164, 1474, 1888
PspFI CCCAGC 3 cut(s) 187, 563, 1394
PspGI CCWGG 5 cut(s) 546, 582, 1164, 1474, 1888
PspN4I GGNNCC 3 cut(s) 954, 1300, 1480
PspPI GGNCC 2 cut(s) 1331, 1886
PstI CTGCAG 1 cut(s) 1114
PsuI RGATCY 5 cut(s) 88, 1036, 1049, 1409, 2481
PsyI GACNNNGTC 3 cut(s) 141, 2327, 2462
PvuII CAGCTG 1 cut(s) 1805
RsaI GTAC 4 cut(s) 685, 922, 1374, 2435
RsaNI GTAC 4 cut(s) 684, 921, 1373, 2434
RseI CAYNNNNRTG 3 cut(s) 600, 2429, 2471
SapI GCTCTTC 2 cut(s) 1791, 2061
SatI GCNGC 8 cut(s) 237, 606, 693, 951, 1245, 1364, 2159, 2571
Sau96I GGNCC 2 cut(s) 1331, 1886
ScaI AGTACT 1 cut(s) 685
SchI GAGTC 4 cut(s) 151, 1463, 1919, 2159
ScrFI CCNGG 5 cut(s) 548, 584, 1166, 1476, 1890
SduI GDGCHC 7 cut(s) 828, 1001, 1077, 1160, 1358, 1823, 1897
SfaNI GCATC 6 cut(s) 801, 1516, 1538, 1618, 1750, 2122
SfcI CTRYAG 3 cut(s) 153, 1110, 1151
SfuI TTCGAA 1 cut(s) 1904
SinI GGWCC 1 cut(s) 1886
SmiMI CAYNNNNRTG 3 cut(s) 600, 2429, 2471
SmlI CTYRAG 2 cut(s) 1076, 1252
SmoI CTYRAG 2 cut(s) 1076, 1252
SsiI CCGC 5 cut(s) 239, 950, 1523, 1843, 2158
SspI AATATT 1 cut(s) 1541
SspMI CTAG 9 cut(s) 92, 224, 366, 458, 1137, 1202, 1283, 1616, 2244
StyD4I CCNGG 5 cut(s) 546, 582, 1164, 1474, 1888
StyI CCWWGG 1 cut(s) 1944
TaaI ACNGT 8 cut(s) 398, 406, 719, 742, 1029, 1188, 1503, 1882
TaiI ACGT 5 cut(s) 1279, 1440, 1662, 1677, 2423
TaqI TCGA 6 cut(s) 390, 401, 647, 1621, 1904, 2322
TatI WGTACW 1 cut(s) 683
TauI GCSGC 2 cut(s) 953, 2161
TfiI GAWTC 7 cut(s) 68, 329, 362, 968, 1552, 1606, 2000
TscAI CASTG 8 cut(s) 420, 489, 724, 952, 1119, 1723, 1780, 2431
TseFI GTSAC 5 cut(s) 986, 1440, 1675, 2464, 2588
TseI GCWGC 6 cut(s) 236, 605, 692, 1244, 1363, 2570
Tsp45I GTSAC 5 cut(s) 986, 1440, 1675, 2464, 2588
TspDTI ATGAA 4 cut(s) 313, 965, 1397, 2580
TspGWI ACGGA 2 cut(s) 1393, 2537
TspRI CASTG 8 cut(s) 420, 489, 724, 952, 1119, 1723, 1780, 2431
Tth111I GACNNNGTC 3 cut(s) 141, 2327, 2462
VpaK11BI GGWCC 1 cut(s) 1886
VspI ATTAAT 1 cut(s) 162
XapI RAATTY 3 cut(s) 930, 1169, 1726
XbaI TCTAGA 3 cut(s) 365, 1201, 1615
XceI RCATGY 3 cut(s) 314, 656, 1244
XcmI CCANNNNNNNNNTGG 2 cut(s) 1172, 2026
XmiI GTMKAC 2 cut(s) 336, 1656
XmnI GAANNNNTTC 1 cut(s) 972
XspI CTAG 9 cut(s) 92, 224, 366, 458, 1137, 1202, 1283, 1616, 2244
ZrmI AGTACT 1 cut(s) 685
Zsp2I ATGCAT 1 cut(s) 1531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.