RLG00000020726
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0007799
Physical Location & Seq
Forward (+)
68002872 .. 68008743
5872 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020726

Sequence Viewer

Length: 2649 bp
ATGGCGTCGATCTTATTCAAGCTAGGGTTTTGCTTATCCCTGCTATGCCTTGTGATTTCGCCTGCCCAATCTGCGGTTATGAGCATAGATCTAGGCTCCGAGTGGCTCAAAGTTGCGGTAGTGAACCTAAAACGAGGGCAGAGCCCGATTTCGGTTGCTATTAATGAAATGTCTAAGCGAAAAACCCCGGTCCTGGTTGCGTTTCACTCAGGTGACCGCCTTATGGGTGAGGAAGCGGCAGGGTTGGTTGCTCGGTACCCGGAGAAAGTGTTTTCTCAGGCAAGAGAGTTGATTGGGAAGCCCTTCAGTCACGGAAAGAGCCTTTTGGACTCGTTGTACTTGCCATTCGACATTACGGAAGATTCTAGGGGAACTGTGAGTTTCAAAATTGATGACAAGGTCAGTACTTACTCGGCTGAGGAACTAGTGGCTATGATCTTGGGTTATGCTGCTAATTTAGCTGAGTTTCATTCGAAAGTGGAAATAAAGGATGCCGTTATTACTGTTCCCCCCTACTTTGGACAAGCTGAGCGGAAGGGGTTAGTCCGGGCGGCACAGTTGGCTGGAATCAATGTTCTTTCTTTGATAAATGAGCATTCTGGTGCTGCATTGCAGTATGGGATTGATAAGGATTTCTCAAATGAGTCGAGGCATGTCATTTTCTACGATATGGGCACCAGCAGTACTTATGCAGCACTTGTATATTTCTCGGCTTATAGTGCCAAGGAGTTTGGAAAGACTGTGTCGGTCAACCAATTTCAGGTCAAGGATGTTAGATGGAACCCAGAACTAGGGGGTCAGAATTTGGAACTACGGTTGGTTGAGCATTTTGCAGATGAGTTCAATAAACAAGTGGGAAATGGGGTTGATGTGAGGAAGTCTCCAAAAGCAATGGCTAAATTGAAGAAACAGGTGAAACGCACAAAAGAAATTCTGAGTGCAAACACAATGGCTCCAATATCTGTTGAATCTCTTTATGATGATCAGGACTTCAGGAGCACCATAACTCGTGAGAAGTTTGAGGAGCTCTGCGAAGATCTGTGGGAGAAATCACTTGTACCTGTTAAAGAAGTGCTTAAGCATTCTGGTTTAAAGGTGGATGAGTTATATGCAGTGGAATTGATAGGAGGTGCTACCAGAGTGCCAAAATTACAGGCTAAGCTTCAGGAATTTCTTGGAAGGAAAGAGTTGGACAGACATCTGGATGCTGATGAAGCTATAGTTCTTGGTGCCGCTTTGCATGCTGCTAATTTAAGCGATGGAATCAAACTGAATCGTAAGTTAGGAATGGTTGATGGTTCATCCTATGGGTTTGTGCTTGAGTTAGATGGCCCTGATCTTCTGAAAGATGACAGCACTAGACAGTTACTCGTCCCGCGAATGAAGAAACTCCCTAGCAAGATGTTCAGATCCTTTACCCATAGCAAAGACTTTGAGGTTTCGCTAGCATATGAGAGTGAAGCTCTTTTACCCCCCGGTGCCACCTCACCTCTATTTGCCAAGTATGCTGTGTCGGGATTGACAGATGCAAGTGAGAAGTATGCATCCCGGAATCTGTCTTCCCCAATCAAAGCAAGTCTGCATTTCTCTTTGAGTAGAAGTGGCATTTTGTCATTTGATCGTGCAGATGCTGTTGTTGAAATAACAGAATGGGTAGAAGTTCCTAAGAAGAATCTGACAGTTGAGAATGCAACTACTGTTTCCCCCAACATATCGAGCGAAACTGGTGCTCAGAACTCCTCAGCAGAAAGTAATGACAACTTGAAAACTGATGATGGGGGAAACAGTAACGCATCCAATGGTACTGTAGAAGAACAAGGGAGCACCGATCTTGGTATTGAAAAGAAGCTGAAGAAGCGGACCTTCAGAGTTGCACTGAAGATTGTTGAGAAGATGGTCGGACCAGCGATGGCTCCTTCAAAAGAATCTCTTGCTGAAGCCAAACGTAAATTAGAGGAATTAGACAAGAAGGATGCAGAGCGGAGGAGAACTGCAGAGTTGAAAAACAACTTGGAAGGATACATATATGCTACTAAAGAAAAGCTTGAAACATTGGAGGAACTTGAAAAGATTTCAACTAGTGAGGAACGCCAAACCTTCATTGGAAAGCTTGATGAGGTGCAAGAATGGCTCTACATGGATGGCGAAGATGCTACTGCTTCAGAGTTTCAGGAACGTCTAGATATGCTAAAAGCTAATGGTGACCCGATATTCTTTAGATTTAAAGAGCTTTCTGCAAGGCCAGAAGCAGTGAAACACGCTCGAAAGTACCTTGTTGAGCTGCAGCAGATTGTGAATGGCTGGGAGTCCAACAAATCTTGGCTTCCGAAAGATCGGATCACTGAGGTTTTGAGTGATGCTGATAAGTTGAAGACTTGGTTGGATGAGAAGGAGGCCGAACAGAAAAAGACTCCTGGATTTAACACGCCAGTTTTCACATCCGAAGATGTATACATGAAGGTGTTTGATCTGCAAGAAGAGGTTGACAGCATTAACAGAATTCCCAAGCCAAAGCCTAAAATTGAGAAACCGACGAGCAATGAAACAGAGAGCACTGGAGAAAAGCCTAAAGACTCAAACATGACTTCTGAAAGTTCCTCACAAGATGACAAGACTGGGCCAGAACCTGAGGGCCATGACGAGCTATGA

Protein Analysis

883

Amino Acids

98.13

Weight (kDa)

5.44

Isoelectric Point (pI)

41.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 27 - 738 9.1e-100 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 255
AccB1I GGYRCC 4 cut(s) 255, 674, 1229, 1478
AccBSI CCGCTC 2 cut(s) 532, 1981
AccI GTMKAC 1 cut(s) 2451
AccII CGCG 1 cut(s) 1378
AclWI GGATC 2 cut(s) 1404, 2345
AcsI RAATTY 4 cut(s) 802, 930, 1169, 2499
AcuI CTGAAG 8 cut(s) 289, 976, 1148, 1849, 1871, 1898, 1956, 2145
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 7 cut(s) 257, 338, 406, 685, 1059, 1804, 2270
AfiI CCNNNNNNNGG 7 cut(s) 73, 151, 193, 223, 518, 760, 791
AflII CTTAAG 1 cut(s) 1076
AhlI ACTAGT 2 cut(s) 424, 2078
AjnI CCWGG 2 cut(s) 192, 2413
AjuI GAANNNNNNNTTGG 4 cut(s) 1994, 2026, 2363, 2395
AleI CACNNNNGTG 1 cut(s) 210
AloI GAACNNNNNNTCC 2 cut(s) 558, 590
Alw21I GWGCWC 5 cut(s) 1001, 1029, 1732, 1826, 2555
Alw26I GTCTC 1 cut(s) 885
AlwI GGATC 2 cut(s) 1404, 2345
AlwNI CAGNNNCTG 2 cut(s) 1631, 2627
AoxI GGCC 5 cut(s) 1330, 2240, 2394, 2618, 2632
ApeKI GCWGC 6 cut(s) 449, 605, 692, 1244, 2281, 2284
ApoI RAATTY 4 cut(s) 802, 930, 1169, 2499
AseI ATTAAT 1 cut(s) 162
Asp700I GAANNNNTTC 1 cut(s) 302
Asp718I GGTACC 1 cut(s) 255
AspS9I GGNCC 6 cut(s) 190, 1331, 1860, 1901, 2618, 2632
AsuC2I CCSGG 5 cut(s) 188, 260, 548, 1476, 1549
AsuHPI GGTGA 5 cut(s) 224, 239, 925, 1479, 2213
AsuII TTCGAA 1 cut(s) 473
AsuNHI GCTAGC 1 cut(s) 1444
AvaII GGWCC 3 cut(s) 190, 1860, 1901
AxyI CCTNAGG 1 cut(s) 2628
BaeGI GKGCMC 1 cut(s) 677
BanI GGYRCC 4 cut(s) 255, 674, 1229, 1478
BanII GRGCYC 2 cut(s) 146, 1029
BarI GAAGNNNNNNTAC 2 cut(s) 1452, 1484
BauI CACGAG 1 cut(s) 1008
BbsI GAAGAC 2 cut(s) 1551, 2378
Bbv12I GWGCWC 5 cut(s) 1001, 1029, 1732, 1826, 2555
BbvCI CCTCAGC 2 cut(s) 417, 1741
BbvI GCAGC 6 cut(s) 436, 592, 704, 1231, 2268, 2296
BccI CCATC 8 cut(s) 771, 1253, 1289, 1322, 1769, 1888, 1903, 2135
BceAI ACGGC 1 cut(s) 479
BcgI CGANNNNNNTGC 2 cut(s) 1709, 1743
BciT130I CCWGG 2 cut(s) 194, 2415
BciVI GTATCC 1 cut(s) 2012
BclI TGATCA 1 cut(s) 982
BcnI CCSGG 5 cut(s) 188, 260, 548, 1476, 1549
BcoDI GTCTC 1 cut(s) 885
BcuI ACTAGT 2 cut(s) 424, 2078
BfmI CTRYAG 4 cut(s) 1218, 1806, 1992, 2282
BfrI CTTAAG 1 cut(s) 1076
BfuI GTATCC 1 cut(s) 2012
BglII AGATCT 2 cut(s) 88, 1036
BisI GCNGC 9 cut(s) 237, 450, 552, 606, 693, 1233, 1245, 2282, 2285
BlpI GCTNAGC 2 cut(s) 528, 1158
BlsI GCNGC 9 cut(s) 238, 451, 553, 607, 694, 1234, 1246, 2283, 2286
BmcAI AGTACT 2 cut(s) 406, 685
Bme1390I CCNGG 7 cut(s) 188, 194, 260, 548, 1476, 1549, 2415
Bme18I GGWCC 3 cut(s) 190, 1860, 1901
BmgT120I GGNCC 6 cut(s) 190, 1331, 1860, 1901, 2618, 2632
BmiI GGNNCC 8 cut(s) 97, 257, 676, 782, 954, 1231, 1480, 1914
BmrFI CCNGG 7 cut(s) 188, 194, 260, 548, 1476, 1549, 2415
BmrI ACTGGG 1 cut(s) 2625
BmsI GCATC 9 cut(s) 481, 1195, 1516, 1553, 1618, 1802, 1963, 2140, 2347
BmtI GCTAGC 1 cut(s) 1448
BmuI ACTGGG 1 cut(s) 2625
BpiI GAAGAC 2 cut(s) 1551, 2378
BplI GAGNNNNNCTC 4 cut(s) 865, 897, 1447, 1479
BpmI CTGGAG 1 cut(s) 2577
Bpu10I CCTNAGC 2 cut(s) 417, 1741
Bpu1102I GCTNAGC 2 cut(s) 528, 1158
Bpu14I TTCGAA 1 cut(s) 473
BpuEI CTTGAG 1 cut(s) 1340
BpuMI CCSGG 5 cut(s) 188, 260, 548, 1476, 1549
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 186, 723, 1474
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 937, 967
Bsc4I CCNNNNNNNGG 7 cut(s) 73, 151, 193, 223, 518, 760, 791
Bse1I ACTGG 4 cut(s) 1729, 2429, 2560, 2620
Bse21I CCTNAGG 1 cut(s) 2628
Bse3DI GCAATG 3 cut(s) 608, 897, 2545
BseBI CCWGG 2 cut(s) 194, 2415
BseDI CCNNGG 3 cut(s) 186, 723, 1474
BseLI CCNNNNNNNGG 7 cut(s) 73, 151, 193, 223, 518, 760, 791
BseMI GCAATG 3 cut(s) 608, 897, 2545
BseNI ACTGG 4 cut(s) 1729, 2429, 2560, 2620
BseRI GAGGAG 3 cut(s) 1037, 1729, 1999
BseSI GKGCMC 1 cut(s) 677
BseXI GCAGC 6 cut(s) 436, 592, 704, 1231, 2268, 2296
BseYI CCCAGC 1 cut(s) 2301
BsgI GTGCAG 1 cut(s) 1644
Bsh1236I CGCG 1 cut(s) 1378
BshFI GGCC 5 cut(s) 1332, 2242, 2396, 2620, 2634
BshNI GGYRCC 4 cut(s) 255, 674, 1229, 1478
BsiHKAI GWGCWC 5 cut(s) 1001, 1029, 1732, 1826, 2555
BsiSI CCGG 5 cut(s) 188, 260, 547, 1476, 1549
BslFI GGGAC 1 cut(s) 1358
BslI CCNNNNNNNGG 7 cut(s) 73, 151, 193, 223, 518, 760, 791
BsmAI GTCTC 1 cut(s) 885
BsmFI GGGAC 1 cut(s) 1358
BsmI GAATGC 3 cut(s) 595, 1081, 1693
BsnI GGCC 5 cut(s) 1332, 2242, 2396, 2620, 2634
Bsp119I TTCGAA 1 cut(s) 473
Bsp1286I GDGCHC 7 cut(s) 146, 677, 1001, 1029, 1732, 1826, 2555
Bsp1720I GCTNAGC 2 cut(s) 528, 1158
BspANI GGCC 5 cut(s) 1332, 2242, 2396, 2620, 2634
BspFNI CGCG 1 cut(s) 1378
BspLI GGNNCC 8 cut(s) 97, 257, 676, 782, 954, 1231, 1480, 1914
BspMAI CTGCAG 2 cut(s) 1996, 2286
BspOI GCTAGC 1 cut(s) 1448
BspPI GGATC 2 cut(s) 1404, 2345
BspT104I TTCGAA 1 cut(s) 473
BspT107I GGYRCC 4 cut(s) 255, 674, 1229, 1478
BspTI CTTAAG 1 cut(s) 1076
BsrBI CCGCTC 2 cut(s) 532, 1981
BsrDI GCAATG 3 cut(s) 608, 897, 2545
BsrI ACTGG 4 cut(s) 1729, 2429, 2560, 2620
BssECI CCNNGG 3 cut(s) 186, 723, 1474
BssNAI GTATAC 1 cut(s) 2452
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1008
BssT1I CCWWGG 1 cut(s) 723
Bst1107I GTATAC 1 cut(s) 2452
Bst2BI CACGAG 1 cut(s) 1008
Bst2UI CCWGG 2 cut(s) 194, 2415
Bst6I CTCTTC 1 cut(s) 2472
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1076
BstBI TTCGAA 1 cut(s) 473
BstC8I GCNNGC 3 cut(s) 63, 1242, 1446
BstEII GGTNACC 2 cut(s) 212, 2201
BstFNI CGCG 1 cut(s) 1378
BstMAI GTCTC 1 cut(s) 885
BstMWI GCNNNNNNNGC 9 cut(s) 71, 458, 560, 719, 1214, 1241, 1505, 1855, 2128
BstNI CCWGG 2 cut(s) 194, 2415
BstNSI RCATGY 2 cut(s) 656, 1244
BstPI GGTNACC 2 cut(s) 212, 2201
BstSCI CCNGG 7 cut(s) 186, 192, 258, 546, 1474, 1547, 2413
BstSFI CTRYAG 4 cut(s) 1218, 1806, 1992, 2282
BstSLI GKGCMC 1 cut(s) 677
BstUI CGCG 1 cut(s) 1378
BstV1I GCAGC 6 cut(s) 436, 592, 704, 1231, 2268, 2296
BstV2I GAAGAC 2 cut(s) 1551, 2378
BstX2I RGATCY 3 cut(s) 88, 1036, 1409
BstYI RGATCY 3 cut(s) 88, 1036, 1409
BstZ17I GTATAC 1 cut(s) 2452
Bsu36I CCTNAGG 1 cut(s) 2628
BsuI GTATCC 1 cut(s) 2012
BsuRI GGCC 5 cut(s) 1332, 2242, 2396, 2620, 2634
BtgZI GCGATG 2 cut(s) 1272, 1922
BtsI GCAGTG 2 cut(s) 1119, 2256
BtsIMutI CAGTG 5 cut(s) 1119, 1874, 2256, 2340, 2553
Cac8I GCNNGC 3 cut(s) 63, 1242, 1446
CaiI CAGNNNCTG 2 cut(s) 1631, 2627
Cfr13I GGNCC 6 cut(s) 190, 1331, 1860, 1901, 2618, 2632
Csp6I GTAC 7 cut(s) 256, 337, 405, 684, 1058, 1803, 2269
CviAII CATG 6 cut(s) 653, 1241, 2137, 2455, 2581, 2636
CviQI GTAC 7 cut(s) 256, 337, 405, 684, 1058, 1803, 2269
DraI TTTAAA 2 cut(s) 1092, 2224
Eam1104I CTCTTC 1 cut(s) 2472
EarI CTCTTC 1 cut(s) 2472
Ecl136II GAGCTC 1 cut(s) 1027
Eco130I CCWWGG 1 cut(s) 723
Eco24I GRGCYC 2 cut(s) 146, 1029
Eco47I GGWCC 3 cut(s) 190, 1860, 1901
Eco53kI GAGCTC 1 cut(s) 1027
Eco57I CTGAAG 8 cut(s) 289, 976, 1148, 1849, 1871, 1898, 1956, 2145
Eco81I CCTNAGG 1 cut(s) 2628
Eco91I GGTNACC 2 cut(s) 212, 2201
EcoICRI GAGCTC 1 cut(s) 1027
EcoO65I GGTNACC 2 cut(s) 212, 2201
EcoRI GAATTC 1 cut(s) 2499
EcoRII CCWGG 2 cut(s) 192, 2413
EcoT14I CCWWGG 1 cut(s) 723
EcoT22I ATGCAT 1 cut(s) 1546
EcoT38I GRGCYC 2 cut(s) 146, 1029
ErhI CCWWGG 1 cut(s) 723
FaeI CATG 6 cut(s) 656, 1244, 2140, 2458, 2584, 2639
FalI AAGNNNNNCTT 8 cut(s) 1059, 1091, 1847, 1879, 1914, 1946, 2028, 2060
FaqI GGGAC 1 cut(s) 1358
FatI CATG 6 cut(s) 652, 1240, 2136, 2454, 2580, 2635
FauI CCCGC 1 cut(s) 1383
FauNDI CATATG 1 cut(s) 1450
FbaI TGATCA 1 cut(s) 982
FblI GTMKAC 1 cut(s) 2451
Fnu4HI GCNGC 9 cut(s) 237, 450, 552, 606, 693, 1233, 1245, 2282, 2285
FriOI GRGCYC 2 cut(s) 146, 1029
Fsp4HI GCNGC 9 cut(s) 237, 450, 552, 606, 693, 1233, 1245, 2282, 2285
GluI GCNGC 9 cut(s) 237, 450, 552, 606, 693, 1233, 1245, 2282, 2285
GsaI CCCAGC 1 cut(s) 2305
GsuI CTGGAG 1 cut(s) 2577
HaeIII GGCC 5 cut(s) 1332, 2242, 2396, 2620, 2634
HapII CCGG 5 cut(s) 188, 260, 547, 1476, 1549
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 6 cut(s) 656, 1244, 2140, 2458, 2584, 2639
HincII GTYRAC 2 cut(s) 751, 2485
HindII GTYRAC 2 cut(s) 751, 2485
HindIII AAGCTT 3 cut(s) 1160, 2042, 2108
HpaII CCGG 5 cut(s) 188, 260, 547, 1476, 1549
HphI GGTGA 5 cut(s) 224, 239, 925, 1479, 2213
Hpy166II GTNNAC 4 cut(s) 124, 751, 2452, 2485
Hpy188III TCNNGA 8 cut(s) 986, 994, 1010, 1166, 1202, 1515, 2171, 2180
Hpy8I GTNNAC 4 cut(s) 124, 751, 2452, 2485
Hpy99I CGWCG 2 cut(s) 10, 2536
HpyCH4IV ACGT 2 cut(s) 1945, 2176
HpyF10VI GCNNNNNNNGC 9 cut(s) 71, 458, 560, 719, 1214, 1241, 1505, 1855, 2128
HpySE526I ACGT 2 cut(s) 1945, 2176
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 6 cut(s) 656, 1244, 2140, 2458, 2584, 2639
KpnI GGTACC 1 cut(s) 259
Ksp22I TGATCA 1 cut(s) 982
LmnI GCTCC 6 cut(s) 101, 958, 996, 1024, 1821, 1918
Lsp1109I GCAGC 6 cut(s) 436, 592, 704, 1231, 2268, 2296
LweI GCATC 9 cut(s) 481, 1195, 1516, 1553, 1618, 1802, 1963, 2140, 2347
MaeII ACGT 2 cut(s) 1945, 2176
MaeIII GTNAC 5 cut(s) 212, 308, 1365, 1787, 2201
MbiI CCGCTC 2 cut(s) 532, 1981
MflI RGATCY 3 cut(s) 88, 1036, 1409
MhlI GDGCHC 7 cut(s) 146, 677, 1001, 1029, 1732, 1826, 2555
MlyI GAGTC 5 cut(s) 323, 653, 2315, 2404, 2567
MmeI TCCRAC 4 cut(s) 1170, 1879, 2334, 2361
Mph1103I ATGCAT 1 cut(s) 1546
MroXI GAANNNNTTC 1 cut(s) 302
MseI TTAA 8 cut(s) 162, 1065, 1077, 1091, 1253, 2223, 2421, 2493
MslI CAYNNNNRTG 4 cut(s) 210, 600, 1203, 2459
MspCI CTTAAG 1 cut(s) 1076
MspI CCGG 5 cut(s) 188, 260, 547, 1476, 1549
MspR9I CCNGG 7 cut(s) 188, 194, 260, 548, 1476, 1549, 2415
Mva1269I GAATGC 3 cut(s) 595, 1081, 1693
MvaI CCWGG 2 cut(s) 194, 2415
MvnI CGCG 1 cut(s) 1378
MwoI GCNNNNNNNGC 9 cut(s) 71, 458, 560, 719, 1214, 1241, 1505, 1855, 2128
NciI CCSGG 5 cut(s) 188, 260, 548, 1476, 1549
NdeI CATATG 1 cut(s) 1450
NheI GCTAGC 1 cut(s) 1444
NlaIII CATG 6 cut(s) 656, 1244, 2140, 2458, 2584, 2639
NlaIV GGNNCC 8 cut(s) 97, 257, 676, 782, 954, 1231, 1480, 1914
NmeAIII GCCGAG 2 cut(s) 392, 689
NmuCI GTSAC 3 cut(s) 212, 308, 2201
NsiI ATGCAT 1 cut(s) 1546
NspI RCATGY 2 cut(s) 656, 1244
NspV TTCGAA 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 210
PaeI GCATGC 1 cut(s) 1244
PctI GAATGC 3 cut(s) 595, 1081, 1693
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 8 cut(s) 362, 567, 968, 1263, 1273, 1552, 1672, 1925
PflFI GACNNNGTC 2 cut(s) 398, 742
PfoI TCCNGGA 2 cut(s) 1547, 2413
PkrI GCNGC 9 cut(s) 238, 451, 553, 607, 694, 1234, 1246, 2283, 2286
PleI GAGTC 5 cut(s) 323, 652, 2314, 2404, 2567
PpsI GAGTC 5 cut(s) 323, 652, 2314, 2404, 2567
PshBI ATTAAT 1 cut(s) 162
Psp124BI GAGCTC 1 cut(s) 1029
Psp6I CCWGG 2 cut(s) 192, 2413
PspEI GGTNACC 2 cut(s) 212, 2201
PspFI CCCAGC 1 cut(s) 2301
PspGI CCWGG 2 cut(s) 192, 2413
PspN4I GGNNCC 8 cut(s) 97, 257, 676, 782, 954, 1231, 1480, 1914
PspPI GGNCC 6 cut(s) 190, 1331, 1860, 1901, 2618, 2632
PstI CTGCAG 2 cut(s) 1996, 2286
PstNI CAGNNNCTG 2 cut(s) 1631, 2627
PsuI RGATCY 3 cut(s) 88, 1036, 1409
PsyI GACNNNGTC 2 cut(s) 398, 742
RsaI GTAC 7 cut(s) 257, 338, 406, 685, 1059, 1804, 2270
RsaNI GTAC 7 cut(s) 256, 337, 405, 684, 1058, 1803, 2269
RseI CAYNNNNRTG 4 cut(s) 210, 600, 1203, 2459
SacI GAGCTC 1 cut(s) 1029
SaqAI TTAA 8 cut(s) 162, 1065, 1077, 1091, 1253, 2223, 2421, 2493
SatI GCNGC 9 cut(s) 237, 450, 552, 606, 693, 1233, 1245, 2282, 2285
Sau96I GGNCC 6 cut(s) 190, 1331, 1860, 1901, 2618, 2632
ScaI AGTACT 2 cut(s) 406, 685
SchI GAGTC 5 cut(s) 323, 653, 2315, 2404, 2567
ScrFI CCNGG 7 cut(s) 188, 194, 260, 548, 1476, 1549, 2415
SduI GDGCHC 7 cut(s) 146, 677, 1001, 1029, 1732, 1826, 2555
SfaNI GCATC 9 cut(s) 481, 1195, 1516, 1553, 1618, 1802, 1963, 2140, 2347
SfcI CTRYAG 4 cut(s) 1218, 1806, 1992, 2282
SfuI TTCGAA 1 cut(s) 473
SinI GGWCC 3 cut(s) 190, 1860, 1901
SmiMI CAYNNNNRTG 4 cut(s) 210, 600, 1203, 2459
SmlI CTYRAG 2 cut(s) 1076, 1319
SmoI CTYRAG 2 cut(s) 1076, 1319
SpeI ACTAGT 2 cut(s) 424, 2078
SphI GCATGC 1 cut(s) 1244
SstI GAGCTC 1 cut(s) 1029
StyD4I CCNGG 7 cut(s) 186, 192, 258, 546, 1474, 1547, 2413
StyI CCWWGG 1 cut(s) 723
TaiI ACGT 2 cut(s) 1948, 2179
TaqI TCGA 6 cut(s) 8, 348, 473, 647, 1715, 2263
TaqII GACCGA 1 cut(s) 736
TatI WGTACW 3 cut(s) 336, 404, 683
TauI GCSGC 3 cut(s) 239, 554, 1235
TfiI GAWTC 8 cut(s) 362, 567, 968, 1263, 1273, 1552, 1672, 1925
Tru1I TTAA 8 cut(s) 162, 1065, 1077, 1091, 1253, 2223, 2421, 2493
Tru9I TTAA 8 cut(s) 162, 1065, 1077, 1091, 1253, 2223, 2421, 2493
TscAI CASTG 5 cut(s) 1119, 1881, 2256, 2347, 2560
TseFI GTSAC 3 cut(s) 212, 308, 2201
TseI GCWGC 6 cut(s) 449, 605, 692, 1244, 2281, 2284
Tsp45I GTSAC 3 cut(s) 212, 308, 2201
TspDTI ATGAA 8 cut(s) 180, 458, 1227, 1290, 1397, 2089, 2471, 2556
TspGWI ACGGA 2 cut(s) 327, 371
TspRI CASTG 5 cut(s) 1119, 1881, 2256, 2347, 2560
Tth111I GACNNNGTC 2 cut(s) 398, 742
Vha464I CTTAAG 1 cut(s) 1076
VpaK11BI GGWCC 3 cut(s) 190, 1860, 1901
VspI ATTAAT 1 cut(s) 162
XapI RAATTY 4 cut(s) 802, 930, 1169, 2499
XbaI TCTAGA 1 cut(s) 2179
XceI RCATGY 2 cut(s) 656, 1244
XcmI CCANNNNNNNNNTGG 1 cut(s) 2099
XmiI GTMKAC 1 cut(s) 2451
XmnI GAANNNNTTC 1 cut(s) 302
ZrmI AGTACT 2 cut(s) 406, 685
Zsp2I ATGCAT 1 cut(s) 1546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.