MD09G1137300.v1.1
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0007799
Physical Location & Seq
Reverse (-)
10631461 .. 10638000
6540 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1137300.v1.1.491

Sequence Viewer

Length: 2661 bp
ATGGCGTCGATCTTATTCAAGCTAGGGTTGTTCTTATCGGTCCTTTGCATTGTGTTTTCGCCTTCTCAATGTGCGGTTATGAGTATAGATCTAGGCTCCGAATGGGTGAAAGTTGCGGTAGTAAACCTAAAGCGCGGGCAGAGCCCGATCACAGTCGCTATCAATGAGATGTCAAAACGAAAATCCCCCAACTTGGTTGCATTTCACTCCGGTGATCGTCTTCTTGGGGAGGAAGCAGCTGGATTGATTGCTCGGTACCCGGAAAAAGTCTATTCTCAAACAAGGGACTTGATTGGAAAACCCTTTAGTTCCAGTAAAAGCCTATTGGATTCATTGTACTTGCCATTCGACGTTACAGAGGATACTACGGGAACTGTTAGTTTCAAAATTGATGATAAAGTGACTACTTATTCAGTCGAGGAACTAACGGCAATGGTCTTAGGTTATGCTGCAAATTTGGCAGAGTTTCATTCGAAAGTGCCTGTAAAGGATGCTGTAATTTCAGTTCCTCCTTACTTTGGGCAAGCAGAGAGAAAGGGGTTACTCCGAGCGGCACAGTTGGCGGGGATTAATGTTCTTGCTTTGATAAATGAGCATTCTGGTGCAGCATTGCAGTATGGGATTGATAAGGATTTCTCAAATGAGTCAAGGCATATAATTTTCTATGATATGGGCACCAGCAGTACCTATGCGGCACTTGTTTATTTCTCGGCATATAATACCAAGGAGTTTGGTAAGACCGTTTCAGTCAACCAGTTTCAGGTCAAGGATGTCAGATGGGACCCACAACTTGGGGGCCAGAACATGGAATTACGGTTGGTGGAGCATTTTGCAGATGAGTTCAATAAACAAGTAGGGAATGGTGTTGATGTGAGGAAGTCCCCAAAAGCAATGGCTAAATTGAAGAAACAGGTCAAGCGTACAAAAGAAATTCTAAGTGCAAACAAAATGGCACCAATATCTGTTGAATCCCTTTATGATGATCGGGACTTCAGGAGTACGATAACTCGTGAAAAGTTTGAGGAGCTCTGCGAAGATCTGTGGGAGAAGTCACTTATACCTCTTAAAGAAGTGCTCAAATATTCTGGTTTAAAGGTAGATGAGATATATGCAGTGGAACTGATAGGAGGAGCTACCAGGGTGCCAAAGCTGCAGGCTAAGCTTCAGGAATATCTTGGAAGGAAAGAGTTGGATAGACATCTGGATGCTGATGAAGCTATAGTTCTAGGTGCAGCATTATATGCTGCTAATTTAAGTGATGGAATCAAGCTGAACCGTAAGTTAGGGATGATTGATGGTTCTACCTTTGGGTTTGTGCTTGAGTTAGATGGCCCCGATCTTCAGAAAGAAGATAGTACTAGGCAGACACTTGTGCAACGGATGAAGAAACTCCCCAGCAAGATGTTCAGGTCCTTTATCCAGAGCAAAGATTTTGAAGTGTCGCTAGCGTACGAGAGTGAAGATCTTTTACCCCCTGGTGCCACCTCTCCTGTATTTGCTCAGTACTCTGTGTCCAGTTTGACAGAGACAAGTGAGAAGTATGCATCACGGAATTTGTCTTCACCCATTAAAGCAAGTCTGCATTTCTCTTTGAGTAGAAGTGGTGTTTTGTCGTTGGATCGTGCAGACGCTGTTATTGAAGTGTCAGAATGGGTGGAAGTTCCTAAGAAGAATCTGAGTGTGGAGAATTCAACTAATGTTGCACCAAACATTTCGACTGAAACTGGTGCTCAGAACTCTTCAGAAGACAGCAATGGCAATACTAATGATGGTGGAAATAGTAACACTTCCAACTCTACTGTAGAAGCGGATGTTGTTATTGAAAAAAAGTTGAAAAAGCGGACCTTTAGGATTCCACTGAAGATTGTTGAGAAAACAGTCGGACCTGCAATGTCTCCTTCAAAAGAATCTCTTGCTGAAGCAAAGCGTAAATTAGAAGAATTAGACAAGAAGGATGCAGAGAGGAGGAGAACGGCAGAGTTGAAAAATAACTTGGAAGGATACATATATGGTACTAAAGAAAAGTTTGAAACATCCGGGGAATTTGAAAAAATTTCAACTAGCGAGGAACGCCAATCCTTTATTGGAAAGCTAGATGAGGTGCAAGAGTGGCTTTACACCGATGGTGAAGATGCTACTGCTTCAGAGTTTCAGGAACGCCTAGAGATGCTAAAAGCTATTGGTGATCCAATATTCTTCAGATTCAAAGAGCTTACCGCACGGCCAGAAGCAGTGGAACATGCTCGAAAGTACCTTGTTGAGGTGCAACAGATTCTAAGTGCATGGGAGTCGAACAAACCCTGGATTCCGAAAGATCGAACAGATGAGGTTGCGAGTGATGCTGACAAGTTGAAGAAGTGGTTGGACGAGAGCGAGGATGAGCAGAAAAAGACTCCTGCACATAGCAAACCAGCATTCACATCGGATGAAATGTTTGGGAAGGTGTTTGATCTGGAAGACAAGGTTGCCAGCGTCAACAGAATCCCCAAGCCAAAGCCTAAAATTGAGAAACCCACAAGCAATGAAACCGAGAGCAGCGGAGAAAAAGCCAAAGATTCTGACTCAAGTTCTGATAATTCCTCACAAGATGACCAGAAGGCCGGAGACTTAGATGACTCAACAAATGAAAAAGTTGAGCCAGCGGGTCACGATGAGCTATGA

Protein Analysis

887

Amino Acids

98.66

Weight (kDa)

5.29

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 26 - 732 1.5e-96 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1894
Acc65I GGTACC 1 cut(s) 255
AccB1I GGYRCC 5 cut(s) 255, 674, 952, 1141, 1478
AccB7I CCANNNNNTGG 2 cut(s) 791, 805
AccBSI CCGCTC 1 cut(s) 551
AccII CGCG 1 cut(s) 135
AclWI GGATC 2 cut(s) 1626, 2180
AcoI YGGCCR 1 cut(s) 2222
AcsI RAATTY 6 cut(s) 454, 930, 1552, 1687, 2042, 2052
AcuI CTGAAG 8 cut(s) 976, 1148, 1325, 1725, 1880, 1938, 2127, 2182
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 6 cut(s) 193, 518, 760, 791, 805, 2260
AjnI CCWGG 3 cut(s) 1136, 1474, 2300
AjuI GAANNNNNNNTTGG 4 cut(s) 1976, 2008, 2345, 2377
AleI CACNNNNGTG 1 cut(s) 210
Alw21I GWGCWC 3 cut(s) 1029, 1077, 1732
Alw26I GTCTC 3 cut(s) 1520, 1899, 2598
AlwI GGATC 2 cut(s) 1626, 2180
AlwNI CAGNNNCTG 1 cut(s) 1631
AoxI GGCC 4 cut(s) 796, 1330, 2222, 2598
ApeKI GCWGC 7 cut(s) 236, 449, 605, 1150, 1232, 1244, 2535
ApoI RAATTY 6 cut(s) 454, 930, 1552, 1687, 2042, 2052
AseI ATTAAT 1 cut(s) 570
Asp718I GGTACC 1 cut(s) 255
AspLEI GCGC 1 cut(s) 135
AspS9I GGNCC 7 cut(s) 40, 781, 796, 1331, 1410, 1842, 1883
AsuC2I CCSGG 2 cut(s) 260, 2038
AsuHPI GGTGA 5 cut(s) 118, 224, 1554, 2138, 2195
AsuII TTCGAA 1 cut(s) 473
AsuNHI GCTAGC 1 cut(s) 1444
AvaII GGWCC 5 cut(s) 40, 781, 1410, 1842, 1883
BaeGI GKGCMC 1 cut(s) 677
BanI GGYRCC 5 cut(s) 255, 674, 952, 1141, 1478
BanII GRGCYC 2 cut(s) 146, 1029
BarI GAAGNNNNNNTAC 2 cut(s) 1452, 1484
BauI CACGAG 1 cut(s) 1008
BbsI GAAGAC 4 cut(s) 212, 1551, 1752, 2463
Bbv12I GWGCWC 3 cut(s) 1029, 1077, 1732
BbvI GCAGC 7 cut(s) 248, 436, 617, 1137, 1231, 1244, 2547
BccI CCATC 6 cut(s) 771, 1253, 1289, 1322, 1763, 2117
BceAI ACGGC 3 cut(s) 444, 1989, 2237
BcgI CGANNNNNNTGC 4 cut(s) 2271, 2305, 2403, 2437
BciT130I CCWGG 3 cut(s) 1138, 1476, 2302
BciVI GTATCC 2 cut(s) 355, 1994
BcnI CCSGG 2 cut(s) 260, 2038
BcoDI GTCTC 3 cut(s) 1520, 1899, 2598
BfaI CTAG 8 cut(s) 23, 92, 1226, 1359, 1445, 2061, 2093, 2162
BfmI CTRYAG 3 cut(s) 1151, 1218, 1800
BfuAI ACCTGC 1 cut(s) 1894
BfuI GTATCC 2 cut(s) 355, 1994
BglII AGATCT 3 cut(s) 88, 1036, 1462
BisI GCNGC 9 cut(s) 237, 450, 552, 606, 693, 1151, 1233, 1245, 2536
BlpI GCTNAGC 1 cut(s) 1158
BlsI GCNGC 9 cut(s) 238, 451, 553, 607, 694, 1152, 1234, 1246, 2537
BmcAI AGTACT 2 cut(s) 1357, 1505
Bme1390I CCNGG 5 cut(s) 260, 1138, 1476, 2038, 2302
Bme18I GGWCC 5 cut(s) 40, 781, 1410, 1842, 1883
BmgT120I GGNCC 7 cut(s) 40, 781, 796, 1331, 1410, 1842, 1883
BmrFI CCNGG 5 cut(s) 260, 1138, 1476, 2038, 2302
BmsI GCATC 7 cut(s) 481, 1195, 1553, 1945, 2122, 2157, 2329
BmtI GCTAGC 1 cut(s) 1448
BpiI GAAGAC 4 cut(s) 212, 1551, 1752, 2463
Bpu1102I GCTNAGC 1 cut(s) 1158
Bpu14I TTCGAA 1 cut(s) 473
BpuEI CTTGAG 2 cut(s) 1340, 2548
BpuMI CCSGG 2 cut(s) 260, 2038
BsaBI GATNNNNATC 1 cut(s) 1197
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 5 cut(s) 723, 1137, 1474, 2037, 2300
BsaWI WCCGGW 1 cut(s) 209
Bsc4I CCNNNNNNNGG 6 cut(s) 193, 518, 760, 791, 805, 2260
Bse1I ACTGG 4 cut(s) 312, 754, 1515, 1729
Bse3DI GCAATG 6 cut(s) 438, 608, 897, 1759, 1896, 2527
Bse8I GATNNNNATC 1 cut(s) 1197
BseBI CCWGG 3 cut(s) 1138, 1476, 2302
BseDI CCNNGG 5 cut(s) 723, 1137, 1474, 2037, 2300
BseJI GATNNNNATC 1 cut(s) 1197
BseLI CCNNNNNNNGG 6 cut(s) 193, 518, 760, 791, 805, 2260
BseMI GCAATG 6 cut(s) 438, 608, 897, 1759, 1896, 2527
BseMII CTCAG 3 cut(s) 1514, 1667, 1745
BseNI ACTGG 4 cut(s) 312, 754, 1515, 1729
BseRI GAGGAG 4 cut(s) 1037, 1143, 1978, 1981
BseSI GKGCMC 1 cut(s) 677
BseXI GCAGC 7 cut(s) 248, 436, 617, 1137, 1231, 1244, 2547
BseYI CCCAGC 1 cut(s) 1394
BsgI GTGCAG 4 cut(s) 624, 1251, 1644, 2382
Bsh1236I CGCG 1 cut(s) 135
BshFI GGCC 4 cut(s) 798, 1332, 2224, 2600
BshNI GGYRCC 5 cut(s) 255, 674, 952, 1141, 1478
BsiHKAI GWGCWC 3 cut(s) 1029, 1077, 1732
BsiSI CCGG 4 cut(s) 210, 260, 2037, 2601
BsiWI CGTACG 1 cut(s) 1449
BslFI GGGAC 4 cut(s) 299, 794, 865, 1001
BslI CCNNNNNNNGG 6 cut(s) 193, 518, 760, 791, 805, 2260
BsmAI GTCTC 3 cut(s) 1520, 1899, 2598
BsmFI GGGAC 4 cut(s) 299, 794, 865, 1001
BsmI GAATGC 2 cut(s) 595, 2414
BsnI GGCC 4 cut(s) 798, 1332, 2224, 2600
Bsp119I TTCGAA 1 cut(s) 473
Bsp1286I GDGCHC 5 cut(s) 146, 677, 1029, 1077, 1732
Bsp1720I GCTNAGC 1 cut(s) 1158
BspANI GGCC 4 cut(s) 798, 1332, 2224, 2600
BspCNI CTCAG 3 cut(s) 1513, 1668, 1744
BspFNI CGCG 1 cut(s) 135
BspMAI CTGCAG 1 cut(s) 1155
BspMI ACCTGC 1 cut(s) 1894
BspOI GCTAGC 1 cut(s) 1448
BspPI GGATC 2 cut(s) 1626, 2180
BspT104I TTCGAA 1 cut(s) 473
BspT107I GGYRCC 5 cut(s) 255, 674, 952, 1141, 1478
BsrBI CCGCTC 1 cut(s) 551
BsrDI GCAATG 6 cut(s) 438, 608, 897, 1759, 1896, 2527
BsrI ACTGG 4 cut(s) 312, 754, 1515, 1729
BssECI CCNNGG 5 cut(s) 723, 1137, 1474, 2037, 2300
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 1008
BssT1I CCWWGG 1 cut(s) 723
Bst2BI CACGAG 1 cut(s) 1008
Bst2UI CCWGG 3 cut(s) 1138, 1476, 2302
Bst4CI ACNGT 8 cut(s) 154, 376, 558, 742, 816, 1277, 1801, 1879
Bst6I CTCTTC 1 cut(s) 1744
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1241
BstBI TTCGAA 1 cut(s) 473
BstC8I GCNNGC 6 cut(s) 137, 525, 1155, 1446, 2470, 2640
BstDEI CTNAG 9 cut(s) 439, 935, 1158, 1500, 1665, 1676, 1731, 2276, 2608
BstENI CCTNNNNNAGG 1 cut(s) 2258
BstFNI CGCG 1 cut(s) 135
BstHHI GCGC 1 cut(s) 135
BstMAI GTCTC 3 cut(s) 1520, 1899, 2598
BstNI CCWGG 3 cut(s) 1138, 1476, 2302
BstNSI RCATGY 1 cut(s) 2243
BstSCI CCNGG 5 cut(s) 258, 1136, 1474, 2036, 2300
BstSFI CTRYAG 3 cut(s) 1151, 1218, 1800
BstSLI GKGCMC 1 cut(s) 677
BstUI CGCG 1 cut(s) 135
BstV1I GCAGC 7 cut(s) 248, 436, 617, 1137, 1231, 1244, 2547
BstV2I GAAGAC 4 cut(s) 212, 1551, 1752, 2463
BstX2I RGATCY 3 cut(s) 88, 1036, 1462
BstYI RGATCY 3 cut(s) 88, 1036, 1462
BsuI GTATCC 2 cut(s) 355, 1994
BsuRI GGCC 4 cut(s) 798, 1332, 2224, 2600
BtsI GCAGTG 2 cut(s) 1119, 2238
BtsIMutI CAGTG 3 cut(s) 1119, 1856, 2238
BveI ACCTGC 1 cut(s) 1894
Cac8I GCNNGC 6 cut(s) 137, 525, 1155, 1446, 2470, 2640
CaiI CAGNNNCTG 1 cut(s) 1631
CfoI GCGC 1 cut(s) 135
Cfr13I GGNCC 7 cut(s) 40, 781, 796, 1331, 1410, 1842, 1883
CseI GACGC 2 cut(s) 1637, 2461
CviAII CATG 3 cut(s) 805, 2240, 2283
DdeI CTNAG 9 cut(s) 439, 935, 1158, 1500, 1665, 1676, 1731, 2276, 2608
DraI TTTAAA 1 cut(s) 1092
EaeI YGGCCR 1 cut(s) 2222
Eam1104I CTCTTC 1 cut(s) 1744
EarI CTCTTC 1 cut(s) 1744
Ecl136II GAGCTC 1 cut(s) 1027
Eco130I CCWWGG 1 cut(s) 723
Eco24I GRGCYC 2 cut(s) 146, 1029
Eco47I GGWCC 5 cut(s) 40, 781, 1410, 1842, 1883
Eco53kI GAGCTC 1 cut(s) 1027
Eco57I CTGAAG 8 cut(s) 976, 1148, 1325, 1725, 1880, 1938, 2127, 2182
EcoICRI GAGCTC 1 cut(s) 1027
EcoNI CCTNNNNNAGG 1 cut(s) 2258
EcoO109I RGGNCCY 2 cut(s) 781, 1410
EcoRI GAATTC 1 cut(s) 1687
EcoRII CCWGG 3 cut(s) 1136, 1474, 2300
EcoT14I CCWWGG 1 cut(s) 723
EcoT22I ATGCAT 1 cut(s) 1546
EcoT38I GRGCYC 2 cut(s) 146, 1029
ErhI CCWWGG 1 cut(s) 723
FaeI CATG 3 cut(s) 808, 2243, 2286
FalI AAGNNNNNCTT 6 cut(s) 1829, 1861, 1896, 1928, 2097, 2129
FaqI GGGAC 4 cut(s) 299, 794, 865, 1001
FatI CATG 3 cut(s) 804, 2239, 2282
FauI CCCGC 3 cut(s) 128, 556, 2635
Fnu4HI GCNGC 9 cut(s) 237, 450, 552, 606, 693, 1151, 1233, 1245, 2536
FriOI GRGCYC 2 cut(s) 146, 1029
Fsp4HI GCNGC 9 cut(s) 237, 450, 552, 606, 693, 1151, 1233, 1245, 2536
FspBI CTAG 8 cut(s) 23, 92, 1226, 1359, 1445, 2061, 2093, 2162
GlaI GCGC 1 cut(s) 134
GluI GCNGC 9 cut(s) 237, 450, 552, 606, 693, 1151, 1233, 1245, 2536
GsaI CCCAGC 1 cut(s) 1398
HaeIII GGCC 4 cut(s) 798, 1332, 2224, 2600
HapII CCGG 4 cut(s) 210, 260, 2037, 2601
HgaI GACGC 2 cut(s) 1637, 2461
HhaI GCGC 1 cut(s) 135
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 808, 2243, 2286
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HincII GTYRAC 2 cut(s) 751, 2476
HindII GTYRAC 2 cut(s) 751, 2476
HindIII AAGCTT 1 cut(s) 1160
HpaII CCGG 4 cut(s) 210, 260, 2037, 2601
HphI GGTGA 5 cut(s) 118, 224, 1554, 2138, 2195
Hpy166II GTNNAC 3 cut(s) 124, 751, 2476
Hpy188III TCNNGA 9 cut(s) 986, 994, 1010, 1166, 1202, 1420, 2153, 2453, 2648
Hpy8I GTNNAC 3 cut(s) 124, 751, 2476
Hpy99I CGWCG 2 cut(s) 10, 353
HpyAV CCTTC 7 cut(s) 72, 1173, 1908, 1945, 1991, 2434, 2590
HpyCH4III ACNGT 8 cut(s) 154, 376, 558, 742, 816, 1277, 1801, 1879
HpyCH4IV ACGT 1 cut(s) 351
HpyF3I CTNAG 9 cut(s) 439, 935, 1158, 1500, 1665, 1676, 1731, 2276, 2608
HpySE526I ACGT 1 cut(s) 351
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 808, 2243, 2286
HspAI GCGC 1 cut(s) 133
KflI GGGWCCC 1 cut(s) 781
KpnI GGTACC 1 cut(s) 259
LmnI GCTCC 4 cut(s) 101, 823, 1024, 1130
Lsp1109I GCAGC 7 cut(s) 248, 436, 617, 1137, 1231, 1244, 2547
LweI GCATC 7 cut(s) 481, 1195, 1553, 1945, 2122, 2157, 2329
MaeI CTAG 8 cut(s) 23, 92, 1226, 1359, 1445, 2061, 2093, 2162
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 6 cut(s) 352, 400, 540, 1050, 1781, 2645
MbiI CCGCTC 1 cut(s) 551
MflI RGATCY 3 cut(s) 88, 1036, 1462
MhlI GDGCHC 5 cut(s) 146, 677, 1029, 1077, 1732
MlyI GAGTC 5 cut(s) 653, 2297, 2386, 2555, 2609
MmeI TCCRAC 5 cut(s) 1170, 1596, 1815, 1861, 2343
Mph1103I ATGCAT 1 cut(s) 1546
MseI TTAA 5 cut(s) 570, 1065, 1091, 1253, 1569
MslI CAYNNNNRTG 3 cut(s) 210, 600, 1203
MspA1I CMGCKG 3 cut(s) 239, 2538, 2642
MspI CCGG 4 cut(s) 210, 260, 2037, 2601
MspR9I CCNGG 5 cut(s) 260, 1138, 1476, 2038, 2302
Mva1269I GAATGC 2 cut(s) 595, 2414
MvaI CCWGG 3 cut(s) 1138, 1476, 2302
MvnI CGCG 1 cut(s) 135
NciI CCSGG 2 cut(s) 260, 2038
NheI GCTAGC 1 cut(s) 1444
NlaIII CATG 3 cut(s) 808, 2243, 2286
NmeAIII GCCGAG 1 cut(s) 689
NmuCI GTSAC 3 cut(s) 400, 1050, 2645
NsiI ATGCAT 1 cut(s) 1546
NspI RCATGY 1 cut(s) 2243
NspV TTCGAA 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 210
PctI GAATGC 2 cut(s) 595, 2414
Pfl23II CGTACG 1 cut(s) 1449
PflMI CCANNNNNTGG 2 cut(s) 791, 805
PkrI GCNGC 9 cut(s) 238, 451, 553, 607, 694, 1152, 1234, 1246, 2537
PleI GAGTC 5 cut(s) 652, 2296, 2386, 2555, 2609
PpsI GAGTC 5 cut(s) 652, 2296, 2386, 2555, 2609
PpuMI RGGWCCY 2 cut(s) 781, 1410
PshBI ATTAAT 1 cut(s) 570
Psp124BI GAGCTC 1 cut(s) 1029
Psp5II RGGWCCY 2 cut(s) 781, 1410
Psp6I CCWGG 3 cut(s) 1136, 1474, 2300
PspFI CCCAGC 1 cut(s) 1394
PspGI CCWGG 3 cut(s) 1136, 1474, 2300
PspLI CGTACG 1 cut(s) 1449
PspPI GGNCC 7 cut(s) 40, 781, 796, 1331, 1410, 1842, 1883
PspPPI RGGWCCY 2 cut(s) 781, 1410
PsrI GAACNNNNNNTAC 2 cut(s) 489, 521
PstI CTGCAG 1 cut(s) 1155
PstNI CAGNNNCTG 1 cut(s) 1631
PsuI RGATCY 3 cut(s) 88, 1036, 1462
PvuII CAGCTG 1 cut(s) 239
RseI CAYNNNNRTG 3 cut(s) 210, 600, 1203
SacI GAGCTC 1 cut(s) 1029
SaqAI TTAA 5 cut(s) 570, 1065, 1091, 1253, 1569
SatI GCNGC 9 cut(s) 237, 450, 552, 606, 693, 1151, 1233, 1245, 2536
Sau96I GGNCC 7 cut(s) 40, 781, 796, 1331, 1410, 1842, 1883
ScaI AGTACT 2 cut(s) 1357, 1505
SchI GAGTC 5 cut(s) 653, 2297, 2386, 2555, 2609
ScrFI CCNGG 5 cut(s) 260, 1138, 1476, 2038, 2302
SduI GDGCHC 5 cut(s) 146, 677, 1029, 1077, 1732
SfaNI GCATC 7 cut(s) 481, 1195, 1553, 1945, 2122, 2157, 2329
SfcI CTRYAG 3 cut(s) 1151, 1218, 1800
SfuI TTCGAA 1 cut(s) 473
SinI GGWCC 5 cut(s) 40, 781, 1410, 1842, 1883
SmiMI CAYNNNNRTG 3 cut(s) 210, 600, 1203
SmlI CTYRAG 2 cut(s) 1319, 2563
SmoI CTYRAG 2 cut(s) 1319, 2563
SspI AATATT 2 cut(s) 1082, 2193
SspMI CTAG 8 cut(s) 23, 92, 1226, 1359, 1445, 2061, 2093, 2162
SstI GAGCTC 1 cut(s) 1029
StyD4I CCNGG 5 cut(s) 258, 1136, 1474, 2036, 2300
StyI CCWWGG 1 cut(s) 723
TaaI ACNGT 8 cut(s) 154, 376, 558, 742, 816, 1277, 1801, 1879
TaiI ACGT 1 cut(s) 354
TaqI TCGA 8 cut(s) 8, 348, 417, 473, 1715, 2245, 2291, 2317
TaqII GACCGA 1 cut(s) 28
TatI WGTACW 3 cut(s) 336, 1355, 1503
TauI GCSGC 2 cut(s) 554, 695
Tru1I TTAA 5 cut(s) 570, 1065, 1091, 1253, 1569
Tru9I TTAA 5 cut(s) 570, 1065, 1091, 1253, 1569
TscAI CASTG 3 cut(s) 1119, 1863, 2238
TseFI GTSAC 3 cut(s) 400, 1050, 2645
TseI GCWGC 7 cut(s) 236, 449, 605, 1150, 1232, 1244, 2535
Tsp45I GTSAC 3 cut(s) 400, 1050, 2645
TspDTI ATGAA 7 cut(s) 321, 458, 1227, 1397, 2442, 2538, 2640
TspGWI ACGGA 2 cut(s) 1393, 1564
TspRI CASTG 3 cut(s) 1119, 1863, 2238
Van91I CCANNNNNTGG 2 cut(s) 791, 805
VpaK11BI GGWCC 5 cut(s) 40, 781, 1410, 1842, 1883
VspI ATTAAT 1 cut(s) 570
XagI CCTNNNNNAGG 1 cut(s) 2258
XapI RAATTY 6 cut(s) 454, 930, 1552, 1687, 2042, 2052
XceI RCATGY 1 cut(s) 2243
XcmI CCANNNNNNNNNTGG 1 cut(s) 2081
XspI CTAG 8 cut(s) 23, 92, 1226, 1359, 1445, 2061, 2093, 2162
ZrmI AGTACT 2 cut(s) 1357, 1505
Zsp2I ATGCAT 1 cut(s) 1546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.