Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0007799

Physical Location & Seq

Genomic Coordinates Forward (+)

67554629 .. 67560675

Gene Structure 6047 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G041530.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2661 bp

ATGCAATCTAGGATGGCGTCGATCTTATTGAAGCTAGGGTTTTGCTTATCCCTCCTATGCCTTGTGGTTTCGCCTGCCCAATCTGCGGTTATGAGCATAGATCTAGGCTCCGAGTGGCTCAAAGTTGCGGTAGTGAACCTAAAACGAGGGCAGAGCCCGATTTCGGTTGCTATTAATGAAATGTCGAAGCGAAAAACCCCGGTCTTGGTTGCCTTTCACTCCGGTGACCGCCTTATGGGTGAGGAAGCGGCAGGGTTGGTTGCTCGGTACCCGGAGAAAGTGTTTTCTCAGGCAAGAGAGTTGATTGGGAAGCCCTTCAGTCACGGAAAGAGCTTTTTGGACTCGTTGTACTTGCCATTCGACATTACGGAAGATTCTAGGGGAACGGTGAGTTTCAAAATTGATGACAAGGTCAGTACTTACTCGGCTGAGGAACTAGTGGCTATGATCTTGGGTTATGCTGCTGATTTAGCTGAGTTTCATTCGAAAGTGGAAATAAAGGATGCCGTTATTACTGTTCCCCCCTACTTTGGACAAGCTGAGCGGAAGGGGTTAGTCCGGGCGGCACAGTTGGCTGGAATCAATGTTCTTTCTTTGATAAATGAGCATTCTGGTGCTGCATTGCAGTATGGGATTGATAAGGATTTCTCAAATGAGTCGAGGCATGTCATTTTCTACGATATGGGCACCAGCAGTACTTATGCAGCACTTGTATATTTCTCGGCTTATAATGCCAAGGAGTTTGGAAAGACTGTGTCGGTCAACCAATTTCAGGTCAAGGATGTCAGATGGAACCCAGAACTAGGGGGTCAGAATTTGGAACTACGGTTGGTTGAGCATTTTGCAGATGAGTTCAATAAACAAGTGGGAAATGGGGTTGATGTGAGGAAGTCTCCAAAAGCAATGGCTAAATTGAAGAAACAGGTGAAACGCACAAAAGAAATTCTGAGTGCAAACACAATGGCTCCAATATCTGTTGAATCTCTTTATGATGATCGGGACTTCAGGAGCACCATAACTCGTGAGAAGTTTGAGGAGCTCTGCGAAGATCTGTGGGAGAAATCACTTGTACCTGTTAAAGAAGTGCTTAAGCATTCTGGTTTAAAGGTGGACGAGTTATATGCAGTGGAGTTGATAGGAGGTGCTACCAGAGTGCCAAAATTACAGGCTAAGCTTCAGGAATTTCTTGGAAGGAAAGAGTTAGACAGACATCTGGATGCTGATGAAGCTATAGTTCTTGGTGCCGCTTTGCATGCTGCTAATTTAAGCGATGGAATCAAACTGAATCGTAAGTTAGGAATGGTTGATGGTTCATCCTATGGGTTTGTGCTTGAGTTAGATGGCCCTGATCTTCTGAAAGATGACAGCACTAGACAGTTACTCGTACCACGAATGAAGAAACTCCCTAGCAAGATGTTCAGATCCTTTACCCATAGCAAAGACTTTGAGGTTTCACTAGCATATGAGAGTGAAGCTCTTTTACCCCCTGGTGCCACCTCTCCTCTATTTGCCAAGTATGCTGTGTCGGGATTGACAGATGCAAGTGAGAAGTATGCATCCCGGAATCTGTCTTCCCCAATCAAAGCAAGTCTGCATTTCTCTTTGAGTAGAAGTGGCATTTTGTCATTTGATCGTGCAGATGCTGTAGTTGAAATAACAGAATGGGTAGAAGTTCCTAAGAAGAATCTGACAGTTGAGAATGCAACTACTGTTTCCCCCAACATATCGAGCGAAACTGGTGCTCAGAACTCCTCAGCAGAAAGTAATGACAACTTGAAAACTGATGATGGGGGAAACAGTAACGCATCCAACGCTACTGTAGAAGAACAAGGTAGCGCCGATCTTGGTATTGAAAAGAAGCTGAAGAAGCGGACCTTCAGAGTTGCACTGAAGATTGTTGAGAAGACGGTCGGACCAGCGATGGCTCCTTCAAAAGAATCTCTTGCTGAAGCCAAACGTAAATTAGAGGAATTAGACAAGAAGGATGCAGAGCGGAGGAAAACTGCAGAGTTGAAAAACAACTTGGAAGGATACATATATGCTACTAAAGAAAAGCTTGAAACATTGGAGGAACTTGAAAAGATTTCAACTAGTGAGGAACGCCAAACCTTCATTGGAAAGCTTGATGAGGTGCAAGAATGGCTCTACATGGATGGCGAAGATGCTACTGCTTCAGAGTTTCAGGAACGTCTAGATATGCTAAAAGCTAATGGTGACCCAATATTCTTTAGATTTAAAGAGCTCTCTGCACGGCCAGAAGCAGTGAAACACGCTCGAAAGTACCTTGTTGAGCTGCAGCAGATTGTGAATGGCTGGGAGTCCAACAAATCTTGGCTTCCAAAAGATCGGATCACTGAGGTTTTGAGTGATGCTGATAAGTTGAAGACTTGGTTGGATGAGAAGGAGGCTGAACAGAAAAAGACTCCTGGATTTAATACGCCAGTTTTCACATCCGAAGATGTATACATGAAGGTGTTTGATCTGCAAGAAGAGGTTGACAGCATTAACAGAATTCCCAAGCCAAAGCCTAAAATTGAGAAACCGACGAGCAATGAAACAGAGAGCACTGGAGAAAAGCCTAAAGACTCAAACATGACTTCTGAAAGTTCCTCACAAGACGACAAGACTGGGTCAGAACCTGAGGGCCATGACGAGCTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0016020 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

HSP70

Protein Analysis

886

Amino Acids

98.59

Weight (kDa)

5.48

Isoelectric Point (pI)

41.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HSP70	PF00012	31 - 742	1.9e-99	Hsp70 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007799)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16660 AT4G16660
fragaria_vesca	FvH4_6g39720 FvH4_6g39720 FvH4_6g39720 FvH4_6g39720 FvH4_6g39720
malus_domestica	MD09G1137300.v1.1 MD17G1127600.v1.1
prunus_persica	Prupe.3G187600_v2.0.a1 Prupe.3G187600_v2.0.a1
pyrus_communis	pycom09g05840 pycom17g11990
rosa_chinensis	RchiOBHm_Chr2g0153811
rosa_laevigata	RLG00000020726
rosa_multiflora	Rmu_sc0013018.1_g000001
rosa_roxburghii	Rroxscaffold_2G00094740
rosa_rugosa	Rorug02G0442100.1 Rorug02G0442200
rosa_samantha	Rh2AG506000 Rh2BG515500 Rh2CG491500 Rh2DG528000
rosa_wichuraiana	Rw2G041530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	729
Acc65I	GGTACC	1 cut(s)	267
AccB1I	GGYRCC	4 cut(s)	267, 686, 1241, 1490
AccBSI	CCGCTC	2 cut(s)	544, 1993
AccI	GTMKAC	1 cut(s)	2463
AciI	CCGC	9 cut(s)	86, 128, 229, 248, 544, 563, 1245, 1870, 1993
AclWI	GGATC	2 cut(s)	1416, 2357
AcoI	YGGCCR	1 cut(s)	2252
AcsI	RAATTY	4 cut(s)	814, 942, 1181, 2511
AcuI	CTGAAG	8 cut(s)	301, 988, 1160, 1861, 1883, 1910, 1968, 2157
AcyI	GRCGYC	1 cut(s)	17
AfaI	GTAC	7 cut(s)	269, 350, 418, 697, 1071, 1386, 2282
AfiI	CCNNNNNNNGG	7 cut(s)	85, 163, 205, 235, 530, 772, 803
AflII	CTTAAG	1 cut(s)	1088
AhlI	ACTAGT	2 cut(s)	436, 2090
AjnI	CCWGG	2 cut(s)	1486, 2425
AjuI	GAANNNNNNNTTGG	4 cut(s)	2006, 2038, 2375, 2407
AleI	CACNNNNGTG	1 cut(s)	222
AloI	GAACNNNNNNTCC	2 cut(s)	570, 602
Alw21I	GWGCWC	5 cut(s)	1013, 1041, 1744, 2244, 2567
Alw26I	GTCTC	1 cut(s)	897
AlwI	GGATC	2 cut(s)	1416, 2357
AlwNI	CAGNNNCTG	2 cut(s)	1643, 2639
AoxI	GGCC	3 cut(s)	1342, 2252, 2644
ApeKI	GCWGC	6 cut(s)	461, 617, 704, 1256, 2293, 2296
ApoI	RAATTY	4 cut(s)	814, 942, 1181, 2511
AseI	ATTAAT	1 cut(s)	174
Asp700I	GAANNNNTTC	1 cut(s)	314
Asp718I	GGTACC	1 cut(s)	267
AspLEI	GCGC	1 cut(s)	1838
AspS9I	GGNCC	4 cut(s)	1343, 1872, 1913, 2644
AsuC2I	CCSGG	4 cut(s)	200, 272, 560, 1561
AsuHPI	GGTGA	5 cut(s)	236, 251, 400, 937, 2225
AsuII	TTCGAA	1 cut(s)	485
AvaII	GGWCC	2 cut(s)	1872, 1913
AxyI	CCTNAGG	1 cut(s)	2640
BaeGI	GKGCMC	1 cut(s)	689
BanI	GGYRCC	4 cut(s)	267, 686, 1241, 1490
BanII	GRGCYC	3 cut(s)	158, 1041, 2244
BarI	GAAGNNNNNNTAC	2 cut(s)	1464, 1496
BauI	CACGAG	1 cut(s)	1020
BbsI	GAAGAC	3 cut(s)	1563, 1910, 2390
Bbv12I	GWGCWC	5 cut(s)	1013, 1041, 1744, 2244, 2567
BbvCI	CCTCAGC	2 cut(s)	429, 1753
BbvI	GCAGC	6 cut(s)	448, 604, 716, 1243, 2280, 2308
BccI	CCATC	8 cut(s)	7, 783, 1265, 1301, 1334, 1781, 1915, 2147
BceAI	ACGGC	2 cut(s)	491, 2267
BcgI	CGANNNNNNTGC	4 cut(s)	1103, 1137, 1721, 1755
BciT130I	CCWGG	2 cut(s)	1488, 2427
BciVI	GTATCC	1 cut(s)	2024
BcnI	CCSGG	4 cut(s)	200, 272, 560, 1561
BcoDI	GTCTC	1 cut(s)	897
BcuI	ACTAGT	2 cut(s)	436, 2090
BfmI	CTRYAG	5 cut(s)	1230, 1644, 1818, 2004, 2294
BfoI	RGCGCY	1 cut(s)	1839
BfrI	CTTAAG	1 cut(s)	1088
BfuI	GTATCC	1 cut(s)	2024
BglII	AGATCT	2 cut(s)	100, 1048
BisI	GCNGC	9 cut(s)	249, 462, 564, 618, 705, 1245, 1257, 2294, 2297
BlpI	GCTNAGC	2 cut(s)	540, 1170
BlsI	GCNGC	9 cut(s)	250, 463, 565, 619, 706, 1246, 1258, 2295, 2298
BmcAI	AGTACT	2 cut(s)	418, 697
Bme1390I	CCNGG	6 cut(s)	200, 272, 560, 1488, 1561, 2427
Bme18I	GGWCC	2 cut(s)	1872, 1913
BmgT120I	GGNCC	4 cut(s)	1343, 1872, 1913, 2644
BmiI	GGNNCC	8 cut(s)	109, 269, 688, 794, 966, 1243, 1492, 1926
BmrFI	CCNGG	6 cut(s)	200, 272, 560, 1488, 1561, 2427
BmrI	ACTGGG	1 cut(s)	2637
BmsI	GCATC	9 cut(s)	493, 1207, 1528, 1565, 1630, 1814, 1975, 2152, 2359
BmuI	ACTGGG	1 cut(s)	2637
BpiI	GAAGAC	3 cut(s)	1563, 1910, 2390
BplI	GAGNNNNNCTC	4 cut(s)	877, 909, 1459, 1491
BpmI	CTGGAG	1 cut(s)	2589
Bpu10I	CCTNAGC	2 cut(s)	429, 1753
Bpu1102I	GCTNAGC	2 cut(s)	540, 1170
Bpu14I	TTCGAA	1 cut(s)	485
BpuEI	CTTGAG	1 cut(s)	1352
BpuMI	CCSGG	4 cut(s)	200, 272, 560, 1561
BsaHI	GRCGYC	1 cut(s)	17
BsaJI	CCNNGG	3 cut(s)	198, 735, 1486
BsaWI	WCCGGW	1 cut(s)	221
BsaXI	ACNNNNNCTCC	4 cut(s)	266, 296, 949, 979
Bsc4I	CCNNNNNNNGG	7 cut(s)	85, 163, 205, 235, 530, 772, 803
Bse1I	ACTGG	4 cut(s)	1741, 2441, 2572, 2632
Bse21I	CCTNAGG	1 cut(s)	2640
Bse3DI	GCAATG	3 cut(s)	620, 909, 2557
BseBI	CCWGG	2 cut(s)	1488, 2427
BseDI	CCNNGG	3 cut(s)	198, 735, 1486
BseLI	CCNNNNNNNGG	7 cut(s)	85, 163, 205, 235, 530, 772, 803
BseMI	GCAATG	3 cut(s)	620, 909, 2557
BseMII	CTCAG	9 cut(s)	302, 420, 465, 531, 938, 1757, 1767, 2346, 2631
BseNI	ACTGG	4 cut(s)	1741, 2441, 2572, 2632
BseRI	GAGGAG	3 cut(s)	1049, 1491, 1741
BseSI	GKGCMC	1 cut(s)	689
BseXI	GCAGC	6 cut(s)	448, 604, 716, 1243, 2280, 2308
BseYI	CCCAGC	1 cut(s)	2313
BsgI	GTGCAG	2 cut(s)	1656, 2232
Bsh1285I	CGRYCG	1 cut(s)	1911
BshFI	GGCC	3 cut(s)	1344, 2254, 2646
BshNI	GGYRCC	4 cut(s)	267, 686, 1241, 1490
BsiEI	CGRYCG	1 cut(s)	1911
BsiHKAI	GWGCWC	5 cut(s)	1013, 1041, 1744, 2244, 2567
BsiSI	CCGG	5 cut(s)	200, 222, 272, 559, 1561
BslFI	GGGAC	1 cut(s)	1013
BslI	CCNNNNNNNGG	7 cut(s)	85, 163, 205, 235, 530, 772, 803
BsmAI	GTCTC	1 cut(s)	897
BsmFI	GGGAC	1 cut(s)	1013
BsmI	GAATGC	3 cut(s)	607, 1093, 1705
BsnI	GGCC	3 cut(s)	1344, 2254, 2646
Bsp119I	TTCGAA	1 cut(s)	485
Bsp1286I	GDGCHC	7 cut(s)	158, 689, 1013, 1041, 1744, 2244, 2567
Bsp1720I	GCTNAGC	2 cut(s)	540, 1170
BspACI	CCGC	9 cut(s)	86, 128, 229, 248, 544, 563, 1245, 1870, 1993
BspANI	GGCC	3 cut(s)	1344, 2254, 2646
BspCNI	CTCAG	9 cut(s)	301, 421, 466, 532, 939, 1756, 1766, 2347, 2632
BspLI	GGNNCC	8 cut(s)	109, 269, 688, 794, 966, 1243, 1492, 1926
BspMAI	CTGCAG	2 cut(s)	2008, 2298
BspPI	GGATC	2 cut(s)	1416, 2357
BspT104I	TTCGAA	1 cut(s)	485
BspT107I	GGYRCC	4 cut(s)	267, 686, 1241, 1490
BspTI	CTTAAG	1 cut(s)	1088
BsrBI	CCGCTC	2 cut(s)	544, 1993
BsrDI	GCAATG	3 cut(s)	620, 909, 2557
BsrI	ACTGG	4 cut(s)	1741, 2441, 2572, 2632
BssECI	CCNNGG	3 cut(s)	198, 735, 1486
BssNAI	GTATAC	1 cut(s)	2464
BssNI	GRCGYC	1 cut(s)	17
BssSI	CACGAG	1 cut(s)	1020
BssT1I	CCWWGG	1 cut(s)	735
Bst1107I	GTATAC	1 cut(s)	2464
Bst2BI	CACGAG	1 cut(s)	1020
Bst2UI	CCWGG	2 cut(s)	1488, 2427
Bst6I	CTCTTC	1 cut(s)	2484
BstACI	GRCGYC	1 cut(s)	17
BstAFI	CTTAAG	1 cut(s)	1088
BstBI	TTCGAA	1 cut(s)	485
BstC8I	GCNNGC	2 cut(s)	75, 1254
BstEII	GGTNACC	2 cut(s)	224, 2213
BstH2I	RGCGCY	1 cut(s)	1839
BstHHI	GCGC	1 cut(s)	1838
BstMAI	GTCTC	1 cut(s)	897
BstMCI	CGRYCG	1 cut(s)	1911
BstNI	CCWGG	2 cut(s)	1488, 2427
BstNSI	RCATGY	2 cut(s)	668, 1256
BstPI	GGTNACC	2 cut(s)	224, 2213
BstSCI	CCNGG	6 cut(s)	198, 270, 558, 1486, 1559, 2425
BstSFI	CTRYAG	5 cut(s)	1230, 1644, 1818, 2004, 2294
BstSLI	GKGCMC	1 cut(s)	689
BstV1I	GCAGC	6 cut(s)	448, 604, 716, 1243, 2280, 2308
BstV2I	GAAGAC	3 cut(s)	1563, 1910, 2390
BstX2I	RGATCY	3 cut(s)	100, 1048, 1421
BstYI	RGATCY	3 cut(s)	100, 1048, 1421
BstZ17I	GTATAC	1 cut(s)	2464
Bsu36I	CCTNAGG	1 cut(s)	2640
BsuI	GTATCC	1 cut(s)	2024
BsuRI	GGCC	3 cut(s)	1344, 2254, 2646
BtgZI	GCGATG	2 cut(s)	1284, 1934
BtsI	GCAGTG	2 cut(s)	1131, 2268
BtsIMutI	CAGTG	5 cut(s)	1131, 1886, 2268, 2352, 2565
Cac8I	GCNNGC	2 cut(s)	75, 1254
CaiI	CAGNNNCTG	2 cut(s)	1643, 2639
CfoI	GCGC	1 cut(s)	1838
Cfr13I	GGNCC	4 cut(s)	1343, 1872, 1913, 2644
CseI	GACGC	1 cut(s)	6
Csp6I	GTAC	7 cut(s)	268, 349, 417, 696, 1070, 1385, 2281
CviAII	CATG	6 cut(s)	665, 1253, 2149, 2467, 2593, 2648
CviQI	GTAC	7 cut(s)	268, 349, 417, 696, 1070, 1385, 2281
DraI	TTTAAA	2 cut(s)	1104, 2236
EaeI	YGGCCR	1 cut(s)	2252
Eam1104I	CTCTTC	1 cut(s)	2484
EarI	CTCTTC	1 cut(s)	2484
Ecl136II	GAGCTC	2 cut(s)	1039, 2242
Eco130I	CCWWGG	1 cut(s)	735
Eco24I	GRGCYC	3 cut(s)	158, 1041, 2244
Eco47I	GGWCC	2 cut(s)	1872, 1913
Eco53kI	GAGCTC	2 cut(s)	1039, 2242
Eco57I	CTGAAG	8 cut(s)	301, 988, 1160, 1861, 1883, 1910, 1968, 2157
Eco81I	CCTNAGG	1 cut(s)	2640
Eco91I	GGTNACC	2 cut(s)	224, 2213
EcoICRI	GAGCTC	2 cut(s)	1039, 2242
EcoO65I	GGTNACC	2 cut(s)	224, 2213
EcoRI	GAATTC	1 cut(s)	2511
EcoRII	CCWGG	2 cut(s)	1486, 2425
EcoT14I	CCWWGG	1 cut(s)	735
EcoT22I	ATGCAT	1 cut(s)	1558
EcoT38I	GRGCYC	3 cut(s)	158, 1041, 2244
ErhI	CCWWGG	1 cut(s)	735
FaeI	CATG	6 cut(s)	668, 1256, 2152, 2470, 2596, 2651
FalI	AAGNNNNNCTT	8 cut(s)	1071, 1103, 1859, 1891, 1926, 1958, 2040, 2072
FaqI	GGGAC	1 cut(s)	1013
FatI	CATG	6 cut(s)	664, 1252, 2148, 2466, 2592, 2647
FauNDI	CATATG	1 cut(s)	1462
FblI	GTMKAC	1 cut(s)	2463
Fnu4HI	GCNGC	9 cut(s)	249, 462, 564, 618, 705, 1245, 1257, 2294, 2297
FriOI	GRGCYC	3 cut(s)	158, 1041, 2244
Fsp4HI	GCNGC	9 cut(s)	249, 462, 564, 618, 705, 1245, 1257, 2294, 2297
GlaI	GCGC	1 cut(s)	1837
GluI	GCNGC	9 cut(s)	249, 462, 564, 618, 705, 1245, 1257, 2294, 2297
GsaI	CCCAGC	1 cut(s)	2317
GsuI	CTGGAG	1 cut(s)	2589
HaeII	RGCGCY	1 cut(s)	1839
HaeIII	GGCC	3 cut(s)	1344, 2254, 2646
HapII	CCGG	5 cut(s)	200, 222, 272, 559, 1561
HgaI	GACGC	1 cut(s)	6
HhaI	GCGC	1 cut(s)	1838
Hin1I	GRCGYC	1 cut(s)	17
Hin1II	CATG	6 cut(s)	668, 1256, 2152, 2470, 2596, 2651
Hin6I	GCGC	1 cut(s)	1836
HinP1I	GCGC	1 cut(s)	1836
HincII	GTYRAC	2 cut(s)	763, 2497
HindII	GTYRAC	2 cut(s)	763, 2497
HindIII	AAGCTT	3 cut(s)	1172, 2054, 2120
HpaII	CCGG	5 cut(s)	200, 222, 272, 559, 1561
HphI	GGTGA	5 cut(s)	236, 251, 400, 937, 2225
Hpy166II	GTNNAC	5 cut(s)	136, 763, 1111, 2464, 2497
Hpy188III	TCNNGA	8 cut(s)	998, 1006, 1022, 1178, 1214, 1527, 2183, 2192
Hpy8I	GTNNAC	5 cut(s)	136, 763, 1111, 2464, 2497
Hpy99I	CGWCG	2 cut(s)	22, 2548
HpyCH4IV	ACGT	2 cut(s)	1957, 2188
HpySE526I	ACGT	2 cut(s)	1957, 2188
Hsp92I	GRCGYC	1 cut(s)	17
Hsp92II	CATG	6 cut(s)	668, 1256, 2152, 2470, 2596, 2651
HspAI	GCGC	1 cut(s)	1836
KpnI	GGTACC	1 cut(s)	271
LmnI	GCTCC	5 cut(s)	113, 970, 1008, 1036, 1930
Lsp1109I	GCAGC	6 cut(s)	448, 604, 716, 1243, 2280, 2308
LweI	GCATC	9 cut(s)	493, 1207, 1528, 1565, 1630, 1814, 1975, 2152, 2359
MaeII	ACGT	2 cut(s)	1957, 2188
MaeIII	GTNAC	5 cut(s)	224, 320, 1377, 1799, 2213
MbiI	CCGCTC	2 cut(s)	544, 1993
MflI	RGATCY	3 cut(s)	100, 1048, 1421
MhlI	GDGCHC	7 cut(s)	158, 689, 1013, 1041, 1744, 2244, 2567
MlyI	GAGTC	5 cut(s)	335, 665, 2327, 2416, 2579
MmeI	TCCRAC	4 cut(s)	1833, 1891, 2346, 2373
Mph1103I	ATGCAT	1 cut(s)	1558
MroXI	GAANNNNTTC	1 cut(s)	314
MseI	TTAA	8 cut(s)	174, 1077, 1089, 1103, 1265, 2235, 2433, 2505
MslI	CAYNNNNRTG	4 cut(s)	222, 612, 1215, 2471
MspCI	CTTAAG	1 cut(s)	1088
MspI	CCGG	5 cut(s)	200, 222, 272, 559, 1561
MspR9I	CCNGG	6 cut(s)	200, 272, 560, 1488, 1561, 2427
Mva1269I	GAATGC	3 cut(s)	607, 1093, 1705
MvaI	CCWGG	2 cut(s)	1488, 2427
NciI	CCSGG	4 cut(s)	200, 272, 560, 1561
NdeI	CATATG	1 cut(s)	1462
NlaIII	CATG	6 cut(s)	668, 1256, 2152, 2470, 2596, 2651
NlaIV	GGNNCC	8 cut(s)	109, 269, 688, 794, 966, 1243, 1492, 1926
NmeAIII	GCCGAG	2 cut(s)	404, 701
NmuCI	GTSAC	3 cut(s)	224, 320, 2213
NsiI	ATGCAT	1 cut(s)	1558
NspI	RCATGY	2 cut(s)	668, 1256
NspV	TTCGAA	1 cut(s)	485
OliI	CACNNNNGTG	1 cut(s)	222
PaeI	GCATGC	1 cut(s)	1256
PctI	GAATGC	3 cut(s)	607, 1093, 1705
PdmI	GAANNNNTTC	1 cut(s)	314
PfeI	GAWTC	8 cut(s)	374, 579, 980, 1275, 1285, 1564, 1684, 1937
PflFI	GACNNNGTC	3 cut(s)	410, 754, 2629
PfoI	TCCNGGA	2 cut(s)	1559, 2425
PkrI	GCNGC	9 cut(s)	250, 463, 565, 619, 706, 1246, 1258, 2295, 2298
PleI	GAGTC	5 cut(s)	335, 664, 2326, 2416, 2579
PpsI	GAGTC	5 cut(s)	335, 664, 2326, 2416, 2579
PshBI	ATTAAT	1 cut(s)	174
PsiI	TTATAA	1 cut(s)	729
Psp124BI	GAGCTC	2 cut(s)	1041, 2244
Psp6I	CCWGG	2 cut(s)	1486, 2425
PspEI	GGTNACC	2 cut(s)	224, 2213
PspFI	CCCAGC	1 cut(s)	2313
PspGI	CCWGG	2 cut(s)	1486, 2425
PspN4I	GGNNCC	8 cut(s)	109, 269, 688, 794, 966, 1243, 1492, 1926
PspPI	GGNCC	4 cut(s)	1343, 1872, 1913, 2644
PstI	CTGCAG	2 cut(s)	2008, 2298
PstNI	CAGNNNCTG	2 cut(s)	1643, 2639
PsuI	RGATCY	3 cut(s)	100, 1048, 1421
PsyI	GACNNNGTC	3 cut(s)	410, 754, 2629
RsaI	GTAC	7 cut(s)	269, 350, 418, 697, 1071, 1386, 2282
RsaNI	GTAC	7 cut(s)	268, 349, 417, 696, 1070, 1385, 2281
RseI	CAYNNNNRTG	4 cut(s)	222, 612, 1215, 2471
SacI	GAGCTC	2 cut(s)	1041, 2244
SaqAI	TTAA	8 cut(s)	174, 1077, 1089, 1103, 1265, 2235, 2433, 2505
SatI	GCNGC	9 cut(s)	249, 462, 564, 618, 705, 1245, 1257, 2294, 2297
Sau96I	GGNCC	4 cut(s)	1343, 1872, 1913, 2644
ScaI	AGTACT	2 cut(s)	418, 697
SchI	GAGTC	5 cut(s)	335, 665, 2327, 2416, 2579
ScrFI	CCNGG	6 cut(s)	200, 272, 560, 1488, 1561, 2427
SduI	GDGCHC	7 cut(s)	158, 689, 1013, 1041, 1744, 2244, 2567
SfaNI	GCATC	9 cut(s)	493, 1207, 1528, 1565, 1630, 1814, 1975, 2152, 2359
SfcI	CTRYAG	5 cut(s)	1230, 1644, 1818, 2004, 2294
SfuI	TTCGAA	1 cut(s)	485
SinI	GGWCC	2 cut(s)	1872, 1913
SmiMI	CAYNNNNRTG	4 cut(s)	222, 612, 1215, 2471
SmlI	CTYRAG	2 cut(s)	1088, 1331
SmoI	CTYRAG	2 cut(s)	1088, 1331
SpeI	ACTAGT	2 cut(s)	436, 2090
SphI	GCATGC	1 cut(s)	1256
SsiI	CCGC	9 cut(s)	86, 128, 229, 248, 544, 563, 1245, 1870, 1993
SspI	AATATT	1 cut(s)	2223
SstI	GAGCTC	2 cut(s)	1041, 2244
StyD4I	CCNGG	6 cut(s)	198, 270, 558, 1486, 1559, 2425
StyI	CCWWGG	1 cut(s)	735
TaiI	ACGT	2 cut(s)	1960, 2191
TaqI	TCGA	7 cut(s)	20, 185, 360, 485, 659, 1727, 2275
TaqII	GACCGA	1 cut(s)	748
TatI	WGTACW	3 cut(s)	348, 416, 695
TauI	GCSGC	3 cut(s)	251, 566, 1247
TfiI	GAWTC	8 cut(s)	374, 579, 980, 1275, 1285, 1564, 1684, 1937
Tru1I	TTAA	8 cut(s)	174, 1077, 1089, 1103, 1265, 2235, 2433, 2505
Tru9I	TTAA	8 cut(s)	174, 1077, 1089, 1103, 1265, 2235, 2433, 2505
TscAI	CASTG	5 cut(s)	1131, 1893, 2268, 2359, 2572
TseFI	GTSAC	3 cut(s)	224, 320, 2213
TseI	GCWGC	6 cut(s)	461, 617, 704, 1256, 2293, 2296
Tsp45I	GTSAC	3 cut(s)	224, 320, 2213
TspDTI	ATGAA	8 cut(s)	192, 470, 1239, 1302, 1409, 2101, 2483, 2568
TspGWI	ACGGA	2 cut(s)	339, 383
TspRI	CASTG	5 cut(s)	1131, 1893, 2268, 2359, 2572
Tth111I	GACNNNGTC	3 cut(s)	410, 754, 2629
Vha464I	CTTAAG	1 cut(s)	1088
VpaK11BI	GGWCC	2 cut(s)	1872, 1913
VspI	ATTAAT	1 cut(s)	174
XapI	RAATTY	4 cut(s)	814, 942, 1181, 2511
XbaI	TCTAGA	1 cut(s)	2191
XceI	RCATGY	2 cut(s)	668, 1256
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2111
XmiI	GTMKAC	1 cut(s)	2463
XmnI	GAANNNNTTC	1 cut(s)	314
ZrmI	AGTACT	2 cut(s)	418, 697
Zsp2I	ATGCAT	1 cut(s)	1558

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.