FvH4_6g39720
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0007799
Physical Location & Seq
Forward (+)
31363160 .. 31369802
6643 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g39720.t5

Sequence Viewer

Length: 2598 bp
ATGCAATCTAGAATGGCGTCGATCTTGTACAAGCTAGGGTTATGCTTATCGCTGCTATGCCTTGTGATTTCACCTGCCCAATCCGCGGTTATGAGCATAGATCTAGGCTCCGAGTGGCTCAAAGTCGCTGTAGTGAACCTGAAACGCGGGCAGAGCCCGATTTCGGTTGCTATTAATGAAATGTCGAAGCGGAAAACCCCGGTTTTGGTTGCGTTTCACTCCGGTGACCGCCTTATGGGTGAGGAAGCGGCGGGGTTGGTTGCCAGGTACCCTGAGAAAGTGTTTTCTCAGGCAAGAGAGTTGATTGGGAAGCCCTTCGGTCATGGCAAGAACTTTTTGGACTCGTTGTACTTGCCATTCGATGTTACGGAAGATTCCAGGGGAACTGTGAGTTTCAAAATTGATGACAAGGTCACTACTTACTCGGCCGAGGAAATAGTGGCTATGATATTGGGTTATGCTGCTAATTTAGCTGAGTTTCATTCAAAAGTAGAAATAAAGGATGCTGTTATTACTGTTCCCCCTTACTTTGGACAAGCCGAGCGGAAGGGACTAGTCCGGGCCGCACAGTTGGCTGGAATCAATGTTCTTTCTTTGATAAACGAGCATTCTGGAGCTGCATTGCAGTATGGGATTGATAAGAATTTTGAGAATAAATCGAGGCATGTCATTTTCTACGATATGGGTACCAGCAGTACTTATGCAGCACTTGTATATTTCTCGGCTTATAATACCAAGGAGTTTGGAAAGACTGTGTCGGTCAACCAATTTCAGGTGAAGGATGTCAGATGGAACCCAGAACTAGGGGGACAGAATTTGGAACTACGGTTGGTTGAGCATTTTGCGGACGAGTTCAATAAACAAGTGGGAAATGGGGTTGATGTGAGGAAATCTCCAAAAGCAATGGCTAAATTGAAGAAGCAGGTGAAGCGCACAAAAGAAATTCTGAGTGCAAACACAATGGCTCCAATATCTGTTGAATCTCTTTATGATGATCGGGACTTCAGGAGCACCATAACTCGTGAGAAGTTTGAGGAGCTCTGTGAAGATCTGTGGGAGAAATCACTTGTACCTGTTAAAGAAGTGCTTAAGCATTCTGGTTTAAAGGTGGACGAGTTATACGCAGTGGAGTTGATAGGAGGTGCTACCAGAGTGCCTAAATTACAGGCTAAGCTTCAGGAATTTCTTGGAAGGAAAGAGTTGGACAGACATCTGGATGCTGATGAAGCTATAGTTCTTGGTGCTGCCTTGCATGCTGCTAATTTGAGCGATGGAATAAAGCTGAATCGTAAGTTGGGAATGGTTGATGGTTCATCCTATGGGTTTGTGCTCGAGTTAGATGGCCCTGATCTTCTGAAAGATGATAGCACCAGACAGTTACTCGTACCACGAATGAAGAAACTCCCTAGCAAGATGTTCAGATTCTTTACCCATAGCAAAGACTTTGAGGTTTCACTATCATATGAGAGTGAAGATCTTTTACCTCCTGGTGCCACCTCTCCTCTATTTGCCAAGTATGCAGTCTTGGGATTGACAGATGCAAGCGAAAAGTATGCATCCCGGAATCTGTCTTCCCCAATTAAAACTAGTCTGCATTTCTCTTTGAGTAGAAGTGTTCCTAAGAAGAATCTGACAGTTGAGAATGCATCTACTGTTTCCCCCAACATATCGAGTGAAACTGGTGGTCAGAACTCCTCAGCAGAAAGTGATGACAATACTGATGATGGGGGAAATGGTAATGCATCCAACTCTACTGCAGAAGTACAAGGTAGTGCCGATCTTGGTATTGAAAAAAAGCTGAAGAAGCGGACATTTAGAGTTCCGCTGAAGATTGTTGAAAAGACCGTCGGACCAGCAATGGCTCTTTCCAAAGAATCTCTTGCTCAAGCGAAACTTAAATTAGAAGAATTGGACAAGAAGGATGCAGAGCGAAGGAGAACAGCAGAGTTGAAAAACAATTTGGAAGGATACATATATGCTACTAAAGAAAAGCTTGAAACATCTGAGGAATTTGAAAAGATTTCAACTAGCGAGGAACGCCAAACATTCATTGGAAAGCTTGATGAGGTGCAAGAATGGCTCTACATGGATGGTGAAGACGCTACTGCTTCAGAGTTTCAGGAACGCCTAGATATGCTAAAAGCTAAGGGTGACCCGATATTCTTCAGATTTAAAGAGCTCTCTGCACTGCCAGAAGCAGTGAAACACGCCCGAAAGTACCTTGTTGAGCTGCAGCAGATTGTAAACGGCTGGGAGTCCAAAAAAGATTGGCTTCCGAAAGATCGTATTACTGAGGTTTTGAGTGACGCTGACAAGTTGAAGACTTGGTTGGATGAGAAGGAGGCCGAACAGAAAAAGACTCCTGGATTTAACACGCCAGCTTTCACATCTGAAGATGTATACATGAAGGTGTTTGATGTACAAGAGAAGGTTGACAGCATTAACAGAATTCCCAAGCCAAAGCCTAAAATTGAGAAACCGACGAGCAATGAAACAGAGAGCACCGGAGAAAAAGCCAAAGACTCAAACACGACTTCTGAAAGTTCCTCACAAGACGACAAGACTGAGTCAGAACGTGAGGGCCATGATGAGCTATGA

Protein Analysis

866

Amino Acids

96.47

Weight (kDa)

5.81

Isoelectric Point (pI)

39.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 31 - 740 6.7e-99 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
AarI CACCTGC 2 cut(s) 82, 913
Acc36I ACCTGC 2 cut(s) 82, 913
Acc65I GGTACC 2 cut(s) 267, 686
AccB1I GGYRCC 3 cut(s) 267, 686, 1490
AccBSI CCGCTC 1 cut(s) 544
AccI GTMKAC 1 cut(s) 2400
AccII CGCG 2 cut(s) 86, 147
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 6 cut(s) 643, 814, 942, 1181, 2009, 2448
AcuI CTGAAG 7 cut(s) 988, 1160, 1820, 1847, 2094, 2149, 2412
AcyI GRCGYC 1 cut(s) 17
AfiI CCNNNNNNNGG 7 cut(s) 85, 163, 205, 235, 530, 772, 803
AflII CTTAAG 1 cut(s) 1088
AhlI ACTAGT 2 cut(s) 553, 1586
AjnI CCWGG 4 cut(s) 263, 377, 1486, 2362
AjuI GAANNNNNNNTTGG 6 cut(s) 1277, 1309, 1943, 1975, 2312, 2344
AleI CACNNNNGTG 1 cut(s) 222
AloI GAACNNNNNNTCC 2 cut(s) 570, 602
Alw21I GWGCWC 5 cut(s) 1013, 1041, 1332, 2181, 2504
Ama87I CYCGRG 1 cut(s) 1331
AoxI GGCC 5 cut(s) 426, 561, 1342, 2343, 2581
ApeKI GCWGC 8 cut(s) 52, 461, 617, 704, 1244, 1256, 2230, 2233
ApoI RAATTY 6 cut(s) 643, 814, 942, 1181, 2009, 2448
AseI ATTAAT 1 cut(s) 174
Asp700I GAANNNNTTC 1 cut(s) 314
Asp718I GGTACC 2 cut(s) 267, 686
AspLEI GCGC 1 cut(s) 933
AspS9I GGNCC 4 cut(s) 561, 1343, 1850, 2581
AsuC2I CCSGG 3 cut(s) 200, 560, 1561
AsuHPI GGTGA 7 cut(s) 63, 236, 251, 787, 937, 2105, 2162
AvaI CYCGRG 1 cut(s) 1331
AvaII GGWCC 1 cut(s) 1850
BanI GGYRCC 3 cut(s) 267, 686, 1490
BanII GRGCYC 3 cut(s) 158, 1041, 2181
BarI GAAGNNNNNNTAC 2 cut(s) 1464, 1496
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 3 cut(s) 1563, 2103, 2327
Bbv12I GWGCWC 5 cut(s) 1013, 1041, 1332, 2181, 2504
BbvCI CCTCAGC 1 cut(s) 1696
BbvI GCAGC 8 cut(s) 39, 448, 604, 716, 1231, 1243, 2217, 2245
BccI CCATC 6 cut(s) 783, 1265, 1301, 1334, 1718, 2084
BceAI ACGGC 1 cut(s) 2263
BcgI CGANNNNNNTGC 2 cut(s) 1535, 1569
BciT130I CCWGG 4 cut(s) 265, 379, 1488, 2364
BciVI GTATCC 1 cut(s) 1961
BcnI CCSGG 3 cut(s) 200, 560, 1561
BcuI ACTAGT 2 cut(s) 553, 1586
BfaI CTAG 9 cut(s) 9, 35, 104, 554, 803, 1407, 1587, 2028, 2129
BfmI CTRYAG 4 cut(s) 129, 1230, 1755, 2231
BfrI CTTAAG 1 cut(s) 1088
BfuAI ACCTGC 2 cut(s) 82, 913
BfuI GTATCC 1 cut(s) 1961
BglII AGATCT 3 cut(s) 100, 1048, 1474
BlpI GCTNAGC 1 cut(s) 1170
BmcAI AGTACT 1 cut(s) 697
Bme1390I CCNGG 7 cut(s) 200, 265, 379, 560, 1488, 1561, 2364
Bme18I GGWCC 1 cut(s) 1850
BmeT110I CYCGRG 1 cut(s) 1331
BmgT120I GGNCC 4 cut(s) 561, 1343, 1850, 2581
BmiI GGNNCC 6 cut(s) 109, 269, 688, 794, 966, 1492
BmrFI CCNGG 7 cut(s) 200, 265, 379, 560, 1488, 1561, 2364
BmsI GCATC 7 cut(s) 493, 1207, 1528, 1565, 1655, 1751, 1912
BpiI GAAGAC 3 cut(s) 1563, 2103, 2327
BplI GAGNNNNNCTC 2 cut(s) 877, 909
BpmI CTGGAG 1 cut(s) 633
Bpu10I CCTNAGC 2 cut(s) 1696, 2145
Bpu1102I GCTNAGC 1 cut(s) 1170
BpuEI CTTGAG 1 cut(s) 1869
BpuMI CCSGG 3 cut(s) 200, 560, 1561
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 5 cut(s) 84, 198, 378, 429, 735
BsaWI WCCGGW 2 cut(s) 221, 2504
BsaXI ACNNNNNCTCC 2 cut(s) 949, 979
Bsc4I CCNNNNNNNGG 7 cut(s) 85, 163, 205, 235, 530, 772, 803
Bse1I ACTGG 1 cut(s) 1684
Bse3DI GCAATG 4 cut(s) 620, 909, 1863, 2494
BseBI CCWGG 4 cut(s) 265, 379, 1488, 2364
BseDI CCNNGG 5 cut(s) 84, 198, 378, 429, 735
BseGI GGATG 9 cut(s) 508, 787, 1222, 1313, 1556, 1742, 1927, 2095, 2338
BseLI CCNNNNNNNGG 7 cut(s) 85, 163, 205, 235, 530, 772, 803
BseMI GCAATG 4 cut(s) 620, 909, 1863, 2494
BseMII CTCAG 8 cut(s) 264, 302, 465, 938, 1710, 1995, 2283, 2556
BseNI ACTGG 1 cut(s) 1684
BseRI GAGGAG 3 cut(s) 1049, 1491, 1684
BseX3I CGGCCG 1 cut(s) 426
BseXI GCAGC 8 cut(s) 39, 448, 604, 716, 1231, 1243, 2217, 2245
BseYI CCCAGC 1 cut(s) 2250
BsgI GTGCAG 1 cut(s) 2169
Bsh1236I CGCG 2 cut(s) 86, 147
Bsh1285I CGRYCG 1 cut(s) 429
BshFI GGCC 5 cut(s) 428, 563, 1344, 2345, 2583
BshNI GGYRCC 3 cut(s) 267, 686, 1490
BsiEI CGRYCG 1 cut(s) 429
BsiHKAI GWGCWC 5 cut(s) 1013, 1041, 1332, 2181, 2504
BsiHKCI CYCGRG 1 cut(s) 1331
BsiSI CCGG 5 cut(s) 200, 222, 559, 1561, 2505
BslFI GGGAC 3 cut(s) 564, 822, 1013
BslI CCNNNNNNNGG 7 cut(s) 85, 163, 205, 235, 530, 772, 803
BsmFI GGGAC 3 cut(s) 564, 822, 1013
BsmI GAATGC 3 cut(s) 607, 1093, 1648
BsnI GGCC 5 cut(s) 428, 563, 1344, 2345, 2583
BsoBI CYCGRG 1 cut(s) 1331
Bsp1286I GDGCHC 6 cut(s) 158, 1013, 1041, 1332, 2181, 2504
Bsp1407I TGTACA 2 cut(s) 27, 2419
Bsp143I GATC 8 cut(s) 21, 100, 994, 1048, 1348, 1474, 1777, 2281
Bsp1720I GCTNAGC 1 cut(s) 1170
BspANI GGCC 5 cut(s) 428, 563, 1344, 2345, 2583
BspCNI CTCAG 8 cut(s) 265, 301, 466, 939, 1709, 1996, 2284, 2557
BspFNI CGCG 2 cut(s) 86, 147
BspLI GGNNCC 6 cut(s) 109, 269, 688, 794, 966, 1492
BspMAI CTGCAG 2 cut(s) 1759, 2235
BspMI ACCTGC 2 cut(s) 82, 913
BspT107I GGYRCC 3 cut(s) 267, 686, 1490
BspTI CTTAAG 1 cut(s) 1088
BsrBI CCGCTC 1 cut(s) 544
BsrDI GCAATG 4 cut(s) 620, 909, 1863, 2494
BsrGI TGTACA 2 cut(s) 27, 2419
BsrI ACTGG 1 cut(s) 1684
BssECI CCNNGG 5 cut(s) 84, 198, 378, 429, 735
BssMI GATC 8 cut(s) 21, 100, 994, 1048, 1348, 1474, 1777, 2281
BssNAI GTATAC 1 cut(s) 2401
BssNI GRCGYC 1 cut(s) 17
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 1 cut(s) 735
Bst1107I GTATAC 1 cut(s) 2401
Bst2BI CACGAG 1 cut(s) 1020
Bst2UI CCWGG 4 cut(s) 265, 379, 1488, 2364
Bst4CI ACNGT 9 cut(s) 388, 517, 570, 754, 828, 1377, 1636, 1654, 1846
BstACI GRCGYC 1 cut(s) 17
BstAFI CTTAAG 1 cut(s) 1088
BstAUI TGTACA 2 cut(s) 27, 2419
BstC8I GCNNGC 4 cut(s) 149, 1254, 1543, 2379
BstDSI CCRYGG 1 cut(s) 84
BstEII GGTNACC 2 cut(s) 224, 2150
BstF5I GGATG 9 cut(s) 508, 787, 1222, 1313, 1556, 1742, 1927, 2095, 2338
BstFNI CGCG 2 cut(s) 86, 147
BstHHI GCGC 1 cut(s) 933
BstKTI GATC 8 cut(s) 24, 103, 997, 1051, 1351, 1477, 1780, 2284
BstMBI GATC 8 cut(s) 21, 100, 994, 1048, 1348, 1474, 1777, 2281
BstMCI CGRYCG 1 cut(s) 429
BstNI CCWGG 4 cut(s) 265, 379, 1488, 2364
BstNSI RCATGY 2 cut(s) 668, 1256
BstPI GGTNACC 2 cut(s) 224, 2150
BstSCI CCNGG 7 cut(s) 198, 263, 377, 558, 1486, 1559, 2362
BstSFI CTRYAG 4 cut(s) 129, 1230, 1755, 2231
BstUI CGCG 2 cut(s) 86, 147
BstV1I GCAGC 8 cut(s) 39, 448, 604, 716, 1231, 1243, 2217, 2245
BstV2I GAAGAC 3 cut(s) 1563, 2103, 2327
BstX2I RGATCY 3 cut(s) 100, 1048, 1474
BstYI RGATCY 3 cut(s) 100, 1048, 1474
BstZ17I GTATAC 1 cut(s) 2401
BstZI CGGCCG 1 cut(s) 426
BsuI GTATCC 1 cut(s) 1961
BsuRI GGCC 5 cut(s) 428, 563, 1344, 2345, 2583
BtgI CCRYGG 1 cut(s) 84
BtgZI GCGATG 1 cut(s) 1284
BtsCI GGATG 9 cut(s) 508, 787, 1222, 1313, 1556, 1742, 1927, 2095, 2338
BtsI GCAGTG 3 cut(s) 1131, 2186, 2205
BtsIMutI CAGTG 3 cut(s) 1131, 2186, 2205
BveI ACCTGC 2 cut(s) 82, 913
Cac8I GCNNGC 4 cut(s) 149, 1254, 1543, 2379
CfoI GCGC 1 cut(s) 933
Cfr13I GGNCC 4 cut(s) 561, 1343, 1850, 2581
Cfr42I CCGCGG 1 cut(s) 87
CseI GACGC 3 cut(s) 6, 2108, 2315
CviAII CATG 6 cut(s) 323, 665, 1253, 2086, 2404, 2585
DpnI GATC 8 cut(s) 23, 102, 996, 1050, 1350, 1476, 1779, 2283
DpnII GATC 8 cut(s) 21, 100, 994, 1048, 1348, 1474, 1777, 2281
DraI TTTAAA 2 cut(s) 1104, 2173
EaeI YGGCCR 1 cut(s) 426
EagI CGGCCG 1 cut(s) 426
Ecl136II GAGCTC 2 cut(s) 1039, 2179
EclXI CGGCCG 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 735
Eco24I GRGCYC 3 cut(s) 158, 1041, 2181
Eco47I GGWCC 1 cut(s) 1850
Eco52I CGGCCG 1 cut(s) 426
Eco53kI GAGCTC 2 cut(s) 1039, 2179
Eco57I CTGAAG 7 cut(s) 988, 1160, 1820, 1847, 2094, 2149, 2412
Eco88I CYCGRG 1 cut(s) 1331
Eco91I GGTNACC 2 cut(s) 224, 2150
EcoICRI GAGCTC 2 cut(s) 1039, 2179
EcoO65I GGTNACC 2 cut(s) 224, 2150
EcoRI GAATTC 1 cut(s) 2448
EcoRII CCWGG 4 cut(s) 263, 377, 1486, 2362
EcoT14I CCWWGG 1 cut(s) 735
EcoT22I ATGCAT 3 cut(s) 1558, 1648, 1744
EcoT38I GRGCYC 3 cut(s) 158, 1041, 2181
ErhI CCWWGG 1 cut(s) 735
FaeI CATG 6 cut(s) 326, 668, 1256, 2089, 2407, 2588
FaqI GGGAC 3 cut(s) 564, 822, 1013
FatI CATG 6 cut(s) 322, 664, 1252, 2085, 2403, 2584
FauI CCCGC 2 cut(s) 140, 244
FauNDI CATATG 1 cut(s) 1462
FblI GTMKAC 1 cut(s) 2400
FokI GGATG 9 cut(s) 515, 794, 1229, 1300, 1543, 1729, 1934, 2102, 2345
FriOI GRGCYC 3 cut(s) 158, 1041, 2181
FspBI CTAG 9 cut(s) 9, 35, 104, 554, 803, 1407, 1587, 2028, 2129
GlaI GCGC 1 cut(s) 932
GsaI CCCAGC 1 cut(s) 2254
GsuI CTGGAG 1 cut(s) 633
HaeIII GGCC 5 cut(s) 428, 563, 1344, 2345, 2583
HapII CCGG 5 cut(s) 200, 222, 559, 1561, 2505
HgaI GACGC 3 cut(s) 6, 2108, 2315
HhaI GCGC 1 cut(s) 933
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 6 cut(s) 326, 668, 1256, 2089, 2407, 2588
Hin6I GCGC 1 cut(s) 931
HinP1I GCGC 1 cut(s) 931
HincII GTYRAC 2 cut(s) 763, 2434
HindII GTYRAC 2 cut(s) 763, 2434
HindIII AAGCTT 3 cut(s) 1172, 1991, 2057
HpaII CCGG 5 cut(s) 200, 222, 559, 1561, 2505
HphI GGTGA 7 cut(s) 63, 236, 251, 787, 937, 2105, 2162
Hpy166II GTNNAC 6 cut(s) 136, 763, 1111, 2245, 2401, 2434
Hpy188III TCNNGA 8 cut(s) 9, 612, 998, 1006, 1022, 1178, 1214, 2120
Hpy8I GTNNAC 6 cut(s) 136, 763, 1111, 2245, 2401, 2434
Hpy99I CGWCG 3 cut(s) 22, 1850, 2485
HpyCH4III ACNGT 9 cut(s) 388, 517, 570, 754, 828, 1377, 1636, 1654, 1846
HpyCH4IV ACGT 1 cut(s) 2575
HpySE526I ACGT 1 cut(s) 2575
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 6 cut(s) 326, 668, 1256, 2089, 2407, 2588
HspAI GCGC 1 cut(s) 931
KpnI GGTACC 2 cut(s) 271, 690
KspI CCGCGG 1 cut(s) 87
Kzo9I GATC 8 cut(s) 21, 100, 994, 1048, 1348, 1474, 1777, 2281
LmnI GCTCC 5 cut(s) 113, 614, 970, 1008, 1036
Lsp1109I GCAGC 8 cut(s) 39, 448, 604, 716, 1231, 1243, 2217, 2245
LweI GCATC 7 cut(s) 493, 1207, 1528, 1565, 1655, 1751, 1912
MaeI CTAG 9 cut(s) 9, 35, 104, 554, 803, 1407, 1587, 2028, 2129
MaeII ACGT 1 cut(s) 2575
MaeIII GTNAC 6 cut(s) 224, 364, 412, 1377, 2150, 2303
MalI GATC 8 cut(s) 23, 102, 996, 1050, 1350, 1476, 1779, 2283
MbiI CCGCTC 1 cut(s) 544
MboI GATC 8 cut(s) 21, 100, 994, 1048, 1348, 1474, 1777, 2281
MflI RGATCY 3 cut(s) 100, 1048, 1474
MhlI GDGCHC 6 cut(s) 158, 1013, 1041, 1332, 2181, 2504
MlyI GAGTC 5 cut(s) 335, 2264, 2353, 2516, 2576
MmeI TCCRAC 4 cut(s) 1182, 1770, 1828, 2310
Mph1103I ATGCAT 3 cut(s) 1558, 1648, 1744
MroXI GAANNNNTTC 1 cut(s) 314
MseI TTAA 9 cut(s) 174, 1077, 1089, 1103, 1581, 1896, 2172, 2370, 2442
MslI CAYNNNNRTG 3 cut(s) 222, 1215, 2408
MspA1I CMGCKG 2 cut(s) 86, 1825
MspCI CTTAAG 1 cut(s) 1088
MspI CCGG 5 cut(s) 200, 222, 559, 1561, 2505
MspR9I CCNGG 7 cut(s) 200, 265, 379, 560, 1488, 1561, 2364
Mva1269I GAATGC 3 cut(s) 607, 1093, 1648
MvaI CCWGG 4 cut(s) 265, 379, 1488, 2364
MvnI CGCG 2 cut(s) 86, 147
NciI CCSGG 3 cut(s) 200, 560, 1561
NdeI CATATG 1 cut(s) 1462
NdeII GATC 8 cut(s) 21, 100, 994, 1048, 1348, 1474, 1777, 2281
NlaIII CATG 6 cut(s) 326, 668, 1256, 2089, 2407, 2588
NlaIV GGNNCC 6 cut(s) 109, 269, 688, 794, 966, 1492
NmeAIII GCCGAG 4 cut(s) 404, 454, 565, 701
NmuCI GTSAC 4 cut(s) 224, 412, 2150, 2303
NsiI ATGCAT 3 cut(s) 1558, 1648, 1744
NspI RCATGY 2 cut(s) 668, 1256
OliI CACNNNNGTG 1 cut(s) 222
PaeI GCATGC 1 cut(s) 1256
PaeR7I CTCGAG 1 cut(s) 1331
PaqCI CACCTGC 2 cut(s) 82, 913
PctI GAATGC 3 cut(s) 607, 1093, 1648
PdmI GAANNNNTTC 1 cut(s) 314
PfeI GAWTC 8 cut(s) 374, 579, 980, 1285, 1422, 1564, 1627, 1874
PflFI GACNNNGTC 3 cut(s) 410, 754, 2566
PfoI TCCNGGA 2 cut(s) 1559, 2362
PleI GAGTC 5 cut(s) 335, 2263, 2353, 2516, 2575
PpsI GAGTC 5 cut(s) 335, 2263, 2353, 2516, 2575
PshBI ATTAAT 1 cut(s) 174
PsiI TTATAA 1 cut(s) 729
Psp124BI GAGCTC 2 cut(s) 1041, 2181
Psp6I CCWGG 4 cut(s) 263, 377, 1486, 2362
PspEI GGTNACC 2 cut(s) 224, 2150
PspFI CCCAGC 1 cut(s) 2250
PspGI CCWGG 4 cut(s) 263, 377, 1486, 2362
PspN4I GGNNCC 6 cut(s) 109, 269, 688, 794, 966, 1492
PspPI GGNCC 4 cut(s) 561, 1343, 1850, 2581
PspXI VCTCGAGB 1 cut(s) 1331
PstI CTGCAG 2 cut(s) 1759, 2235
PsuI RGATCY 3 cut(s) 100, 1048, 1474
PsyI GACNNNGTC 3 cut(s) 410, 754, 2566
RseI CAYNNNNRTG 3 cut(s) 222, 1215, 2408
SacI GAGCTC 2 cut(s) 1041, 2181
SacII CCGCGG 1 cut(s) 87
SaqAI TTAA 9 cut(s) 174, 1077, 1089, 1103, 1581, 1896, 2172, 2370, 2442
Sau3AI GATC 8 cut(s) 21, 100, 994, 1048, 1348, 1474, 1777, 2281
Sau96I GGNCC 4 cut(s) 561, 1343, 1850, 2581
ScaI AGTACT 1 cut(s) 697
SchI GAGTC 5 cut(s) 335, 2264, 2353, 2516, 2576
ScrFI CCNGG 7 cut(s) 200, 265, 379, 560, 1488, 1561, 2364
SduI GDGCHC 6 cut(s) 158, 1013, 1041, 1332, 2181, 2504
SfaNI GCATC 7 cut(s) 493, 1207, 1528, 1565, 1655, 1751, 1912
SfcI CTRYAG 4 cut(s) 129, 1230, 1755, 2231
Sfr274I CTCGAG 1 cut(s) 1331
Sfr303I CCGCGG 1 cut(s) 87
SgrBI CCGCGG 1 cut(s) 87
SinI GGWCC 1 cut(s) 1850
SlaI CTCGAG 1 cut(s) 1331
SmiMI CAYNNNNRTG 3 cut(s) 222, 1215, 2408
SmlI CTYRAG 3 cut(s) 1088, 1331, 1884
SmoI CTYRAG 3 cut(s) 1088, 1331, 1884
SpeI ACTAGT 2 cut(s) 553, 1586
SphI GCATGC 1 cut(s) 1256
SspMI CTAG 9 cut(s) 9, 35, 104, 554, 803, 1407, 1587, 2028, 2129
SstI GAGCTC 2 cut(s) 1041, 2181
StyD4I CCNGG 7 cut(s) 198, 263, 377, 558, 1486, 1559, 2362
StyI CCWWGG 1 cut(s) 735
TaaI ACNGT 9 cut(s) 388, 517, 570, 754, 828, 1377, 1636, 1654, 1846
TaiI ACGT 1 cut(s) 2578
TaqI TCGA 6 cut(s) 20, 185, 360, 659, 1332, 1670
TaqII GACCGA 2 cut(s) 308, 748
TatI WGTACW 5 cut(s) 27, 348, 695, 1762, 2419
TauI GCSGC 2 cut(s) 251, 566
TfiI GAWTC 8 cut(s) 374, 579, 980, 1285, 1422, 1564, 1627, 1874
Tru1I TTAA 9 cut(s) 174, 1077, 1089, 1103, 1581, 1896, 2172, 2370, 2442
Tru9I TTAA 9 cut(s) 174, 1077, 1089, 1103, 1581, 1896, 2172, 2370, 2442
TscAI CASTG 3 cut(s) 1131, 2193, 2205
TseFI GTSAC 4 cut(s) 224, 412, 2150, 2303
TseI GCWGC 8 cut(s) 52, 461, 617, 704, 1244, 1256, 2230, 2233
Tsp45I GTSAC 4 cut(s) 224, 412, 2150, 2303
TspDTI ATGAA 8 cut(s) 192, 470, 1239, 1302, 1409, 2038, 2420, 2505
TspGWI ACGGA 1 cut(s) 383
TspRI CASTG 3 cut(s) 1131, 2193, 2205
Tth111I GACNNNGTC 3 cut(s) 410, 754, 2566
Vha464I CTTAAG 1 cut(s) 1088
VpaK11BI GGWCC 1 cut(s) 1850
VspI ATTAAT 1 cut(s) 174
XapI RAATTY 6 cut(s) 643, 814, 942, 1181, 2009, 2448
XbaI TCTAGA 1 cut(s) 8
XceI RCATGY 2 cut(s) 668, 1256
XcmI CCANNNNNNNNNTGG 1 cut(s) 2048
XhoI CTCGAG 1 cut(s) 1331
XmiI GTMKAC 1 cut(s) 2400
XmnI GAANNNNTTC 1 cut(s) 314
XspI CTAG 9 cut(s) 9, 35, 104, 554, 803, 1407, 1587, 2028, 2129
ZrmI AGTACT 1 cut(s) 697
Zsp2I ATGCAT 3 cut(s) 1558, 1648, 1744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.