MD17G1127600.v1.1
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007799
Physical Location & Seq
Reverse (-)
11184142 .. 11191753
7612 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1127600.v1.1.491

Sequence Viewer

Length: 2676 bp
ATGCAATCTAGGATGGCGTCGATCCTAATCAAGCTAGGGTTATTCCTATCTGTGCTTTGCCTCATGTTTTCGCCTTCTCAATGTGCGGTTATGAGTATAGATCTAGGCTCTGAATGGGTGAAAGTTGCGGTAGTGAACCTTAAGCGCGGGCAGAGCCCGATCACAGTCGCTATTAACGAGATGTCGAAGCGAAAGTCCCCTAACTTGGTTGCATTTCATTCTGGTGACCGCCTTCTAGGTGAGGAAGCAGCTGGATTGGTTGCTCGGTACCCGGAGAAAGTCTACTCTCAAACAAGGGACTTGATTGGAAAACCCTTTAGTTCCAGCAAAACCTTTTTGGATTCATTGTACTTGCCATTTGACGTTACAGAGGATACTACGGGAACTGTTATTTTCAAAATTGATGATAAAGTGACTACTTATTCAGTCGAGGAACTAGTGGCGATGATTTTAGGTTATGCTGCAAATTTGGCAGAGTTTCATTCAAAAGTGCCCGTAAGGGATGCTGTAATTTCAGTTCCTCCGTACTTTGGGCAAGCAGAGAGAAAGGGGTTACTTCGAGCGGCACAGTTGGTGGGGATTAATGTTCTTGCTTTGATAAATGAGCATTCTGGTGCAGCATTGCAGTATGGGATTGACAAGGATTTCTCAAATGAGTCAAGGCATGTAATTTTCTATGATATGGGCACCAGCAGTACCTATGCGGCACTTGTTTATTTCTCGGCATATAATGCCAAGGAGTTTGGAAAGACCGTGTCAGTCAACCAGTTTCAGGTCAAGGATGTCAGATGGAACCCAGAACTTGGGGGCCAGAACTTGGAATTACAGTTGGTGGAGTATTTTGCAGAGGAGTTCAATAAACAAGTAGGGAATGGTGTTGATGTGAGGAAGTCTCCAAAAGCAATGGCTAAATTGAAGAAACAGGTCAAGCGTACAAAAGAAATTCTAAGTGCAAACAAAATGGCTCCAATATCTGTTGAATCCCTTTATGATGATCGGGACTTCAGGAGCACGATAACTCGTGAAAAGTTTGAAGAGCTCTGTGAAGATCTGTGGGAGAAGTCGCTAGTACCTCTTAAGGAAGTGCTCAAGCATTCTGGTTTAAAGGTAGATGAGATATATGCAGTGGAACTGATAGGAGGAGCTACCAGGGTGCCAAAGTTGCAGGCTAAGCTTCAGGAATATCTTGGAAGGAAAGAGTTGGATAGACATCTGGATGCTGATGAAGCTACAGTTCTTGGTGCAGCATTATATGCTGCTAATTTAAGTGATGGAATCAAACTGAACCGGAAGTTAGGGATGATTGATGGTTCTACCTATGGGTTTGTGCTTGAGTTAGATGGCCCCGATCTTCAGAAAGAAGATAGCACTAGGCAGACACTTGTGCAACGGATGAAGAAACTCCCCAGCAAGATGTTCAGGTCCTTTATCCAGAGCAAAGATTTTGAAGTGACACTAGCGTACGAGAGTGAAGACCTTTTACCCCCTGGTGCCACCTCTCCTGTATTTGCTCAGTACTCTGTGTCCAGTTTGACAGAGACAAGTGAGAAGTATGCATCACGAAATTTGTCTTCACCCATTAAGGCAAGTCTGCATTTCTCTTTGAGTAGAAGTGGTGTTTTGTCGTTGGATCGTGCAGATGCTTTTATTGAAGTGTCAGAATGGGTAGAAGTTCCTAAGAAGAATCTGACTACTCAGGAGAATTCAACTAATGTTGCACCCAACATATCCACTGAAACTGGTGCTCAGAACACATCAAAAGAAAGCAATGGCAATACTGATGATGGTGGAAATAGTAACAGTTCCAACTCTACAGTAGAAACAGACGTCGTTATTGAAAAAAAGCTGAAAAAGCGGACCTTTAGGATTCCACTGAAGATTGTTGAGAAAACAGTCGGACCTGCGATGTCTCCTTCAAAAGAATCTCTTGCTGAAGCAAAACGTAAATTAGAAGAATTAGACAAGAAGGATGCAGAGCGGAGGAGAACGGCAGAGTTGAAAAATACCTTGGAAGGATACATATATGGTATTAAAGAAAAGTTTGAAACATCCAAGGAATATGAAAAGGTTTCAACTAGCGAGGAGCGCCAATCCTTTATTGGAAAGCTAGATGAGGTGCAAGAGTGGCTTTACACCGATGGTGAAGATGCTACTGCTTCAGAGTTTCAGGAACGCCTAGATGTGCTAAAAGCTATTGGTGATCCGATATTCTTCAGATTAAAAGAGCTTACTGCTCGGCCAGAAGCAGTGGAACACGCTCGAAAGTACCTTGTTGAAGTGCAACAGATTCTACGTGGATGGGAGTCAAACAAACCCTGGGTTCCGAAGGATCGAACAGATGAGGTTGCAAGTGATGCTGACAAGTTGAAGAAGTGGTTGGACGAGAAGGAGAATGCGCAGAAAAAGACTCCTGCACATAGCAAACCAGTATTCACATCGGATGAAGTGTTCGGGAAGTTGTTTGATCTGGAAGATAAGGTTGCTAGCGTCAACAGAATCCCCAAGCCAAAGCCTAAAATTGAGAAACCCACAAGCAATGAAACCGAGAGCAGCGGAGAAAAAGCTAAGGATTCTGATTCGAGTTCTGATAATTCTTCACAAGAGGACCAGAAAGCCGGAGACTCAGATGACTCAGCAAATGAAAAAGTTGAGTCCGAGGGTCACGATGAGCTGTGA

Protein Analysis

892

Amino Acids

99.48

Weight (kDa)

5.47

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 30 - 737 1.3e-93 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1830
Acc16I TGCGCA 1 cut(s) 2397
Acc36I ACCTGC 1 cut(s) 1909
Acc65I GGTACC 1 cut(s) 267
AccB1I GGYRCC 4 cut(s) 267, 686, 1153, 1490
AccB7I CCANNNNNTGG 2 cut(s) 803, 817
AccBSI CCGCTC 2 cut(s) 563, 1978
AccI GTMKAC 1 cut(s) 282
AccII CGCG 1 cut(s) 147
AciI CCGC 9 cut(s) 86, 128, 147, 229, 563, 704, 1855, 1978, 2553
AclWI GGATC 4 cut(s) 16, 1638, 2195, 2337
AcoI YGGCCR 1 cut(s) 2237
AcsI RAATTY 4 cut(s) 466, 942, 1564, 1702
AcuI CTGAAG 7 cut(s) 988, 1160, 1337, 1895, 1953, 2142, 2197
AcyI GRCGYC 2 cut(s) 17, 1827
AfaI GTAC 9 cut(s) 269, 350, 527, 697, 934, 1071, 1463, 1517, 2267
AfiI CCNNNNNNNGG 5 cut(s) 205, 530, 772, 803, 817
AflII CTTAAG 2 cut(s) 140, 1076
AhlI ACTAGT 1 cut(s) 436
AjnI CCWGG 3 cut(s) 1148, 1486, 2315
AjuI GAANNNNNNNTTGG 4 cut(s) 1991, 2023, 2360, 2392
AloI GAACNNNNNNTCC 2 cut(s) 2432, 2464
Alw21I GWGCWC 4 cut(s) 1013, 1041, 1089, 1747
Alw26I GTCTC 4 cut(s) 897, 1532, 1914, 2613
AlwI GGATC 4 cut(s) 16, 1638, 2195, 2337
AoxI GGCC 3 cut(s) 808, 1342, 2237
ApeKI GCWGC 6 cut(s) 248, 461, 617, 1244, 1256, 2550
ApoI RAATTY 4 cut(s) 466, 942, 1564, 1702
ArsI GACNNNNNNTTYG 2 cut(s) 179, 211
AseI ATTAAT 1 cut(s) 582
Asp718I GGTACC 1 cut(s) 267
AspLEI GCGC 3 cut(s) 147, 2088, 2398
AspS9I GGNCC 6 cut(s) 808, 1343, 1422, 1857, 1898, 2605
AsuC2I CCSGG 1 cut(s) 272
AsuHPI GGTGA 6 cut(s) 130, 236, 251, 1566, 2153, 2210
AsuNHI GCTAGC 1 cut(s) 2483
AvaII GGWCC 4 cut(s) 1422, 1857, 1898, 2605
BaeGI GKGCMC 2 cut(s) 495, 689
BanI GGYRCC 4 cut(s) 267, 686, 1153, 1490
BanII GRGCYC 2 cut(s) 158, 1041
BarI GAAGNNNNNNTAC 2 cut(s) 1464, 1496
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 2 cut(s) 1479, 1563
Bbv12I GWGCWC 4 cut(s) 1013, 1041, 1089, 1747
BbvI GCAGC 6 cut(s) 260, 448, 629, 1243, 1256, 2562
BccI CCATC 8 cut(s) 7, 783, 1265, 1301, 1334, 1778, 2132, 2292
BceAI ACGGC 1 cut(s) 2004
BciT130I CCWGG 3 cut(s) 1150, 1488, 2317
BciVI GTATCC 2 cut(s) 367, 2009
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 4 cut(s) 897, 1532, 1914, 2613
BcuI ACTAGT 1 cut(s) 436
BfmI CTRYAG 2 cut(s) 1230, 1812
BfoI RGCGCY 1 cut(s) 2089
BfrI CTTAAG 2 cut(s) 140, 1076
BfuAI ACCTGC 1 cut(s) 1909
BfuI GTATCC 2 cut(s) 367, 2009
BglII AGATCT 2 cut(s) 100, 1048
BisI GCNGC 8 cut(s) 249, 462, 564, 618, 705, 1245, 1257, 2551
BlpI GCTNAGC 1 cut(s) 1170
BlsI GCNGC 8 cut(s) 250, 463, 565, 619, 706, 1246, 1258, 2552
BmcAI AGTACT 1 cut(s) 1517
Bme1390I CCNGG 4 cut(s) 272, 1150, 1488, 2317
Bme18I GGWCC 4 cut(s) 1422, 1857, 1898, 2605
BmgT120I GGNCC 6 cut(s) 808, 1343, 1422, 1857, 1898, 2605
BmiI GGNNCC 9 cut(s) 269, 688, 794, 809, 966, 1155, 1345, 1492, 2322
BmrFI CCNGG 4 cut(s) 272, 1150, 1488, 2317
BmsI GCATC 7 cut(s) 493, 1207, 1565, 1630, 1960, 2137, 2344
BmtI GCTAGC 1 cut(s) 2487
BpiI GAAGAC 2 cut(s) 1479, 1563
BplI GAGNNNNNCTC 2 cut(s) 877, 909
Bpu10I CCTNAGC 1 cut(s) 2565
Bpu1102I GCTNAGC 1 cut(s) 1170
BpuEI CTTGAG 2 cut(s) 1073, 1352
BpuMI CCSGG 1 cut(s) 272
BsaAI YACGTR 1 cut(s) 2294
BsaBI GATNNNNATC 2 cut(s) 26, 1209
BsaHI GRCGYC 2 cut(s) 17, 1827
BsaJI CCNNGG 8 cut(s) 735, 1149, 1486, 2007, 2052, 2315, 2316, 2655
BsaWI WCCGGW 1 cut(s) 1287
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 530, 772, 803, 817
Bse1I ACTGG 4 cut(s) 766, 1527, 1744, 2426
Bse3DI GCAATG 4 cut(s) 620, 909, 1774, 2542
Bse8I GATNNNNATC 2 cut(s) 26, 1209
BseBI CCWGG 3 cut(s) 1150, 1488, 2317
BseDI CCNNGG 8 cut(s) 735, 1149, 1486, 2007, 2052, 2315, 2316, 2655
BseJI GATNNNNATC 2 cut(s) 26, 1209
BseLI CCNNNNNNNGG 5 cut(s) 205, 530, 772, 803, 817
BseMI GCAATG 4 cut(s) 620, 909, 1774, 2542
BseMII CTCAG 5 cut(s) 1526, 1709, 1760, 2637, 2646
BseNI ACTGG 4 cut(s) 766, 1527, 1744, 2426
BseRI GAGGAG 4 cut(s) 863, 1155, 1996, 2096
BseSI GKGCMC 2 cut(s) 495, 689
BseXI GCAGC 6 cut(s) 260, 448, 629, 1243, 1256, 2562
BseYI CCCAGC 1 cut(s) 1406
BsgI GTGCAG 4 cut(s) 636, 1263, 1656, 2397
Bsh1236I CGCG 1 cut(s) 147
BshFI GGCC 3 cut(s) 810, 1344, 2239
BshNI GGYRCC 4 cut(s) 267, 686, 1153, 1490
BsiHKAI GWGCWC 4 cut(s) 1013, 1041, 1089, 1747
BsiSI CCGG 3 cut(s) 272, 1288, 2616
BsiWI CGTACG 1 cut(s) 1461
BslFI GGGAC 3 cut(s) 181, 311, 1013
BslI CCNNNNNNNGG 5 cut(s) 205, 530, 772, 803, 817
BsmAI GTCTC 4 cut(s) 897, 1532, 1914, 2613
BsmFI GGGAC 3 cut(s) 181, 311, 1013
BsmI GAATGC 3 cut(s) 607, 1093, 2398
BsnI GGCC 3 cut(s) 810, 1344, 2239
Bsp1286I GDGCHC 7 cut(s) 158, 495, 689, 1013, 1041, 1089, 1747
Bsp1720I GCTNAGC 1 cut(s) 1170
BspACI CCGC 9 cut(s) 86, 128, 147, 229, 563, 704, 1855, 1978, 2553
BspANI GGCC 3 cut(s) 810, 1344, 2239
BspCNI CTCAG 5 cut(s) 1525, 1708, 1759, 2636, 2645
BspFNI CGCG 1 cut(s) 147
BspLI GGNNCC 9 cut(s) 269, 688, 794, 809, 966, 1155, 1345, 1492, 2322
BspMI ACCTGC 1 cut(s) 1909
BspOI GCTAGC 1 cut(s) 2487
BspPI GGATC 4 cut(s) 16, 1638, 2195, 2337
BspQI GCTCTTC 1 cut(s) 1029
BspT107I GGYRCC 4 cut(s) 267, 686, 1153, 1490
BspTI CTTAAG 2 cut(s) 140, 1076
BsrBI CCGCTC 2 cut(s) 563, 1978
BsrDI GCAATG 4 cut(s) 620, 909, 1774, 2542
BsrI ACTGG 4 cut(s) 766, 1527, 1744, 2426
BssECI CCNNGG 8 cut(s) 735, 1149, 1486, 2007, 2052, 2315, 2316, 2655
BssNI GRCGYC 2 cut(s) 17, 1827
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 3 cut(s) 735, 2007, 2052
Bst2BI CACGAG 1 cut(s) 1020
Bst2UI CCWGG 3 cut(s) 1150, 1488, 2317
Bst4CI ACNGT 9 cut(s) 166, 388, 570, 754, 828, 1234, 1802, 1816, 1894
Bst6I CTCTTC 1 cut(s) 1029
BstACI GRCGYC 2 cut(s) 17, 1827
BstAFI CTTAAG 2 cut(s) 140, 1076
BstAPI GCANNNNNTGC 3 cut(s) 731, 1253, 2354
BstBAI YACGTR 1 cut(s) 2294
BstC8I GCNNGC 4 cut(s) 149, 537, 1167, 2485
BstDEI CTNAG 9 cut(s) 947, 1170, 1512, 1677, 1695, 1746, 2565, 2623, 2632
BstEII GGTNACC 1 cut(s) 224
BstFNI CGCG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 2089
BstHHI GCGC 3 cut(s) 147, 2088, 2398
BstMAI GTCTC 4 cut(s) 897, 1532, 1914, 2613
BstNI CCWGG 3 cut(s) 1150, 1488, 2317
BstNSI RCATGY 1 cut(s) 668
BstPI GGTNACC 1 cut(s) 224
BstSCI CCNGG 4 cut(s) 270, 1148, 1486, 2315
BstSFI CTRYAG 2 cut(s) 1230, 1812
BstSLI GKGCMC 2 cut(s) 495, 689
BstUI CGCG 1 cut(s) 147
BstV1I GCAGC 6 cut(s) 260, 448, 629, 1243, 1256, 2562
BstV2I GAAGAC 2 cut(s) 1479, 1563
BstX2I RGATCY 2 cut(s) 100, 1048
BstYI RGATCY 2 cut(s) 100, 1048
BsuI GTATCC 2 cut(s) 367, 2009
BsuRI GGCC 3 cut(s) 810, 1344, 2239
BtgZI GCGATG 2 cut(s) 458, 1919
BtsI GCAGTG 2 cut(s) 1131, 2253
BtsIMutI CAGTG 4 cut(s) 1131, 1731, 1871, 2253
BveI ACCTGC 1 cut(s) 1909
Cac8I GCNNGC 4 cut(s) 149, 537, 1167, 2485
CfoI GCGC 3 cut(s) 147, 2088, 2398
Cfr13I GGNCC 6 cut(s) 808, 1343, 1422, 1857, 1898, 2605
CseI GACGC 2 cut(s) 6, 2476
Csp6I GTAC 9 cut(s) 268, 349, 526, 696, 933, 1070, 1462, 1516, 2266
CviAII CATG 2 cut(s) 64, 665
CviQI GTAC 9 cut(s) 268, 349, 526, 696, 933, 1070, 1462, 1516, 2266
DdeI CTNAG 9 cut(s) 947, 1170, 1512, 1677, 1695, 1746, 2565, 2623, 2632
DraI TTTAAA 1 cut(s) 1104
EaeI YGGCCR 1 cut(s) 2237
Eam1104I CTCTTC 1 cut(s) 1029
EarI CTCTTC 1 cut(s) 1029
Ecl136II GAGCTC 1 cut(s) 1039
Eco130I CCWWGG 3 cut(s) 735, 2007, 2052
Eco24I GRGCYC 2 cut(s) 158, 1041
Eco47I GGWCC 4 cut(s) 1422, 1857, 1898, 2605
Eco53kI GAGCTC 1 cut(s) 1039
Eco57I CTGAAG 7 cut(s) 988, 1160, 1337, 1895, 1953, 2142, 2197
Eco91I GGTNACC 1 cut(s) 224
EcoICRI GAGCTC 1 cut(s) 1039
EcoO109I RGGNCCY 1 cut(s) 1422
EcoO65I GGTNACC 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 1702
EcoRII CCWGG 3 cut(s) 1148, 1486, 2315
EcoT14I CCWWGG 3 cut(s) 735, 2007, 2052
EcoT22I ATGCAT 1 cut(s) 1558
EcoT38I GRGCYC 2 cut(s) 158, 1041
ErhI CCWWGG 3 cut(s) 735, 2007, 2052
FaeI CATG 2 cut(s) 67, 668
FalI AAGNNNNNCTT 6 cut(s) 1844, 1876, 1911, 1943, 2112, 2144
FaqI GGGAC 3 cut(s) 181, 311, 1013
FatI CATG 2 cut(s) 63, 664
FauI CCCGC 1 cut(s) 140
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 8 cut(s) 249, 462, 564, 618, 705, 1245, 1257, 2551
FriOI GRGCYC 2 cut(s) 158, 1041
Fsp4HI GCNGC 8 cut(s) 249, 462, 564, 618, 705, 1245, 1257, 2551
FspI TGCGCA 1 cut(s) 2397
GlaI GCGC 3 cut(s) 146, 2087, 2397
GluI GCNGC 8 cut(s) 249, 462, 564, 618, 705, 1245, 1257, 2551
GsaI CCCAGC 1 cut(s) 1410
HaeII RGCGCY 1 cut(s) 2089
HaeIII GGCC 3 cut(s) 810, 1344, 2239
HapII CCGG 3 cut(s) 272, 1288, 2616
HgaI GACGC 2 cut(s) 6, 2476
HhaI GCGC 3 cut(s) 147, 2088, 2398
Hin1I GRCGYC 2 cut(s) 17, 1827
Hin1II CATG 2 cut(s) 67, 668
Hin6I GCGC 3 cut(s) 145, 2086, 2396
HinP1I GCGC 3 cut(s) 145, 2086, 2396
HincII GTYRAC 2 cut(s) 763, 2491
HindII GTYRAC 2 cut(s) 763, 2491
HindIII AAGCTT 1 cut(s) 1172
HpaII CCGG 3 cut(s) 272, 1288, 2616
HphI GGTGA 6 cut(s) 130, 236, 251, 1566, 2153, 2210
Hpy166II GTNNAC 4 cut(s) 136, 283, 763, 2491
Hpy8I GTNNAC 4 cut(s) 136, 283, 763, 2491
Hpy99I CGWCG 2 cut(s) 22, 1832
HpyAV CCTTC 8 cut(s) 84, 242, 1185, 1923, 1960, 2006, 2320, 2380
HpyCH4III ACNGT 9 cut(s) 166, 388, 570, 754, 828, 1234, 1802, 1816, 1894
HpyCH4IV ACGT 4 cut(s) 363, 1827, 1942, 2293
HpyF3I CTNAG 9 cut(s) 947, 1170, 1512, 1677, 1695, 1746, 2565, 2623, 2632
HpySE526I ACGT 4 cut(s) 363, 1827, 1942, 2293
Hsp92I GRCGYC 2 cut(s) 17, 1827
Hsp92II CATG 2 cut(s) 67, 668
HspAI GCGC 3 cut(s) 145, 2086, 2396
KpnI GGTACC 1 cut(s) 271
LguI GCTCTTC 1 cut(s) 1029
LmnI GCTCC 4 cut(s) 970, 1008, 1142, 2083
Lsp1109I GCAGC 6 cut(s) 260, 448, 629, 1243, 1256, 2562
LweI GCATC 7 cut(s) 493, 1207, 1565, 1630, 1960, 2137, 2344
MaeII ACGT 4 cut(s) 363, 1827, 1942, 2293
MaeIII GTNAC 7 cut(s) 224, 364, 412, 552, 1450, 1796, 2660
MbiI CCGCTC 2 cut(s) 563, 1978
MflI RGATCY 2 cut(s) 100, 1048
MhlI GDGCHC 7 cut(s) 158, 495, 689, 1013, 1041, 1089, 1747
MlyI GAGTC 6 cut(s) 665, 2312, 2401, 2615, 2624, 2660
MmeI TCCRAC 5 cut(s) 1182, 1608, 1830, 1876, 2358
Mph1103I ATGCAT 1 cut(s) 1558
MseI TTAA 9 cut(s) 141, 174, 582, 1077, 1103, 1265, 1581, 2031, 2219
MslI CAYNNNNRTG 3 cut(s) 222, 612, 1215
MspA1I CMGCKG 2 cut(s) 251, 2553
MspCI CTTAAG 2 cut(s) 140, 1076
MspI CCGG 3 cut(s) 272, 1288, 2616
MspR9I CCNGG 4 cut(s) 272, 1150, 1488, 2317
Mva1269I GAATGC 3 cut(s) 607, 1093, 2398
MvaI CCWGG 3 cut(s) 1150, 1488, 2317
MvnI CGCG 1 cut(s) 147
NciI CCSGG 1 cut(s) 272
NheI GCTAGC 1 cut(s) 2483
NlaIII CATG 2 cut(s) 67, 668
NlaIV GGNNCC 9 cut(s) 269, 688, 794, 809, 966, 1155, 1345, 1492, 2322
NmeAIII GCCGAG 2 cut(s) 701, 2215
NmuCI GTSAC 4 cut(s) 224, 412, 1450, 2660
NsbI TGCGCA 1 cut(s) 2397
NsiI ATGCAT 1 cut(s) 1558
NspI RCATGY 1 cut(s) 668
PasI CCCWGGG 1 cut(s) 2316
PciSI GCTCTTC 1 cut(s) 1029
PcsI WCGNNNNNNNCGW 1 cut(s) 174
PctI GAATGC 3 cut(s) 607, 1093, 2398
Pfl23II CGTACG 1 cut(s) 1461
PflFI GACNNNGTC 1 cut(s) 754
PflMI CCANNNNNTGG 2 cut(s) 803, 817
PkrI GCNGC 8 cut(s) 250, 463, 565, 619, 706, 1246, 1258, 2552
PleI GAGTC 6 cut(s) 664, 2311, 2401, 2615, 2624, 2659
PpsI GAGTC 6 cut(s) 664, 2311, 2401, 2615, 2624, 2659
Ppu21I YACGTR 1 cut(s) 2294
PpuMI RGGWCCY 1 cut(s) 1422
PshBI ATTAAT 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 1041
Psp5II RGGWCCY 1 cut(s) 1422
Psp6I CCWGG 3 cut(s) 1148, 1486, 2315
PspEI GGTNACC 1 cut(s) 224
PspFI CCCAGC 1 cut(s) 1406
PspGI CCWGG 3 cut(s) 1148, 1486, 2315
PspLI CGTACG 1 cut(s) 1461
PspN4I GGNNCC 9 cut(s) 269, 688, 794, 809, 966, 1155, 1345, 1492, 2322
PspPI GGNCC 6 cut(s) 808, 1343, 1422, 1857, 1898, 2605
PspPPI RGGWCCY 1 cut(s) 1422
PsrI GAACNNNNNNTAC 2 cut(s) 501, 533
PsuI RGATCY 2 cut(s) 100, 1048
PsyI GACNNNGTC 1 cut(s) 754
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 9 cut(s) 269, 350, 527, 697, 934, 1071, 1463, 1517, 2267
RsaNI GTAC 9 cut(s) 268, 349, 526, 696, 933, 1070, 1462, 1516, 2266
RseI CAYNNNNRTG 3 cut(s) 222, 612, 1215
SacI GAGCTC 1 cut(s) 1041
SapI GCTCTTC 1 cut(s) 1029
SaqAI TTAA 9 cut(s) 141, 174, 582, 1077, 1103, 1265, 1581, 2031, 2219
SatI GCNGC 8 cut(s) 249, 462, 564, 618, 705, 1245, 1257, 2551
Sau96I GGNCC 6 cut(s) 808, 1343, 1422, 1857, 1898, 2605
ScaI AGTACT 1 cut(s) 1517
SchI GAGTC 6 cut(s) 665, 2312, 2401, 2615, 2624, 2660
ScrFI CCNGG 4 cut(s) 272, 1150, 1488, 2317
SduI GDGCHC 7 cut(s) 158, 495, 689, 1013, 1041, 1089, 1747
SfaNI GCATC 7 cut(s) 493, 1207, 1565, 1630, 1960, 2137, 2344
SfcI CTRYAG 2 cut(s) 1230, 1812
SinI GGWCC 4 cut(s) 1422, 1857, 1898, 2605
SmiMI CAYNNNNRTG 3 cut(s) 222, 612, 1215
SmlI CTYRAG 4 cut(s) 140, 1076, 1088, 1331
SmoI CTYRAG 4 cut(s) 140, 1076, 1088, 1331
SpeI ACTAGT 1 cut(s) 436
SsiI CCGC 9 cut(s) 86, 128, 147, 229, 563, 704, 1855, 1978, 2553
SstI GAGCTC 1 cut(s) 1041
StyD4I CCNGG 4 cut(s) 270, 1148, 1486, 2315
StyI CCWWGG 3 cut(s) 735, 2007, 2052
TaaI ACNGT 9 cut(s) 166, 388, 570, 754, 828, 1234, 1802, 1816, 1894
TaiI ACGT 4 cut(s) 366, 1830, 1945, 2296
TaqI TCGA 7 cut(s) 20, 185, 429, 559, 2260, 2332, 2579
TatI WGTACW 2 cut(s) 348, 1515
TauI GCSGC 2 cut(s) 566, 707
Tru1I TTAA 9 cut(s) 141, 174, 582, 1077, 1103, 1265, 1581, 2031, 2219
Tru9I TTAA 9 cut(s) 141, 174, 582, 1077, 1103, 1265, 1581, 2031, 2219
TscAI CASTG 4 cut(s) 1131, 1738, 1878, 2253
TseFI GTSAC 4 cut(s) 224, 412, 1450, 2660
TseI GCWGC 6 cut(s) 248, 461, 617, 1244, 1256, 2550
Tsp45I GTSAC 4 cut(s) 224, 412, 1450, 2660
TspDTI ATGAA 9 cut(s) 206, 333, 470, 1239, 1409, 2076, 2457, 2553, 2655
TspGWI ACGGA 2 cut(s) 513, 1405
TspRI CASTG 4 cut(s) 1131, 1738, 1878, 2253
Tth111I GACNNNGTC 1 cut(s) 754
Van91I CCANNNNNTGG 2 cut(s) 803, 817
Vha464I CTTAAG 2 cut(s) 140, 1076
VpaK11BI GGWCC 4 cut(s) 1422, 1857, 1898, 2605
VspI ATTAAT 1 cut(s) 582
XapI RAATTY 4 cut(s) 466, 942, 1564, 1702
XceI RCATGY 1 cut(s) 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 2096
XmiI GTMKAC 1 cut(s) 282
ZraI GACGTC 1 cut(s) 1828
ZrmI AGTACT 1 cut(s) 1517
Zsp2I ATGCAT 1 cut(s) 1558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.