Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0014415

Physical Location & Seq

Genomic Coordinates Forward (+)

10883921 .. 10888270

Gene Structure 4350 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G20140.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3750 bp

ATGCAACCACTAGTTCTTCTCCTCCTCTTCATCCTCTGTTTTTCCGGTTTAGGTCAACCGGGTATCATCAACAACGATCTTCAGACTCTTCTCGAGGTGAAGAAATCTTTGGTCACGAATCCACAAGAAGACGATCCTCTCCGACAATGGAACTCTGATAACATCAACTACTGTTCTTGGACCGGAGTCACATGCGACAATACCGGTTTATTTCGTGTAATTGCTCTAAACCTTACCGGTTTAGGATTAACCGGTTCAATTTCACCTTGGTTTGGCCGGTTTGACAACCTAATCCACCTTGATCTATCTTCCAACAATCTAGTTGGTCCCATCCCAACTGCTCTCTCCAACCTTACTTCCTTGGAGTCTCTGTTTCTCTTTTCTAACCAACTCACCGGCGAGATTCCGAGCCAGCTCGGCTCCCTCGTTAATATCCGGTCGCTAAGGATCGGAGACAATGAGCTTGTTGGAGATATCCCGGAGACGTTAGGGAACTTGGTGAATCTCCAGATGCTTGCTCTGGCTTCTTGTAGACTCACCGGTCCTATACCGAGTCAGCTCGGTCGTCTCGTCCGAGTTCAGTCGTTGATATTGCAAGATAACTATCTCGAAGGACCGATTCCGGCTGAGTTAGGAAACTGCTCTGATCTCACTGTGTTCACTGCGGCGGAGAACATGCTCAACGGGACGATACCGGCGGAGCTTGGACGCCTTGAAAACCTCGAAATACTCAACCTGGCGAACAACAGTCTCACCGGAGAGATACCGAGTCAATTAGGTGAAATGAGCCAACTCCAGTATCTTAGCTTGATGGCAAATCAACTTCAAGGTCTTATCCCCAAGAGTTTGGCTGATCTGGGAAATCTTCAAACTCTTGATTTATCGGCGAATAATCTTACCGGAGAGATACCTGAAGAATTCTGGAACATGAGTCAGCTTCTTGATTTGGTTCTAGCAAACAATCATCTCTCTGGTTCATTACCAAAGAGCATTTGTTCTAACAACACAAACTTGGAGCAACTGGTTTTGTCTGGAACTCAGCTTTCTGGTGAAATCCCTGTGGAATTAAGCAAATGTCAATCGCTTAAGCAGCTTGATTTGTCGAACAATTCTCTCGCTGGCTCGATCCCTGAAGCATTGTTTGAGCTAGTCGAACTCACAGATCTTTATCTCCACAATAATACCTTGGAGGGTACGTTATCTCCTTCGATATCAAACCTCACTAATCTACAGTGGCTTGTGTTGTATCACAATAACTTGGAAGGCAAGTTACCGAAGGAGATCAGCGCACTCAGAAAATTAGAGGTTCTGTTTCTGTATGAGAATCGTTTCTCAGGGGAAATCCCACAGGAGATTGGAAACTGCACAAGCTTGAAGATGATTGATATGTTCGGGAATCATTTCGAAGGAGAGATACCTCCTTCAATAGGAAGATTAAAAGAGCTTAACTTGCTTCACTTGAGACAGAACGAGCTTGTGGGTGGACTTCCCGCCAGTTTAGGTAACTGTCATCAACTGAACATCTTGGATTTGGCGGATAATCAGCTCTCGGGATCAATTCCTTCATCCTTCGGGTTCTTAAAGGGCTTGGAACAGTTGATGCTTTACAACAATTCTCTTCAAGGAAATCTTCCTGATTCACTTATCAGTCTGAGGAACCTCACCAGAATCAATCTCTCTCACAACAGGCTCAATGGTACGATTCATCCATTATGTGGTTCGAGCTCATATCTCTCATTCGATGTCACCAACAACGGGTTTGAAGATGAAATCCCCCTGGAGCTAGGAAACTCTCAAAATCTTGATCGTCTCAGGTTAGGCAAGAATCAATTGACAGGAAAAATCCCTTGGACGTTGGGGAAAATCCGCGAGCTGTCCTTGTTAGATATGTCAAGCAACGCTCTCACAGGAACCATTCCGCTACAGCTCGTCTTGTGCAAGAAACTGACACACATCGATCTCAATAACAATTTTCTATCAGGTCCTATACCTCCATGGCTTGGAAAACTTTCACAGTTAGGAGAGCTCAAGCTTTCTTCGAATCAGTTTGTTGAGTCTTTACCTACTGAACTGTTCAACTGTACAAAGCTGCTTGTGTTGTCTCTTGATGGAAATTCGCTTAATGGGTCGATTCCACAAGAGATTGGTAACTTGGGAGCTCTCAATGTCCTTAACCTTGATAAGAACCAGTTTTCAGGTTCACTTCCTCAAGCTATGGGAAAGCTCAGCAAGCTGTATGAGCTTCGGTTATCAAGAAACAGCTTAACAGGAGAAATCCCAGTTGAGATTGGACAGCTTCAGGATCTTCAAAGTGCTTTAGACCTCAGCTACAATAACTTCACTGGAGATATACCTTCGACAATTGGGACATTATCAAAGCTGGAAACACTTGATCTTTCTCACAATCAGCTTACAGGGGAAGTTCCTGGCTCGGTTGGGGATATGAAGAGTTTGGGATATCTCAATGTTTCTTTCAATAACCTCGGAGGGAAGCTCAAGAAACAGTTCTCAAGATGGCCAGCTGATTCCTTTTTAGGCAACACAGGTCTTTGTGGAAGCCCACTAAGTCGCTGCAACAGAGTTAGAAGTAATAACAAGCAACAAGGTCTTAGTGCCAGATCAGTTGTGATAATATCAGCAATCTCAGCATTGACGGCGATTGGCTTGATGATACTTGTAATCGCTCTCTTCTTCAAACAAAGGCATGATTTCTTCAAGAAAGTGGGTCATGGAAGCACTGCGTACACATCGTCAAGCTCTTCTTCACAAGCTACACACAAGCCTCTCTTTCGAAACGGAGCTTCAAAGTCCGATATCAGGTGGGAGGACATCATGGAAGCTACACATAATCTAAGCGAGGAGTTCATGATTGGATCAGGAGGCTCTGGGAAGGTTTACAAGGCGGAGCTTGAAAATGGTGAGACTGTTGCAGTGAAAAAGATTCTCTGGAAGGATGATCTCATGTCAAACAAGAGCTTCAGCAGAGAAGTTAAGACGCTTGGAAGAATCAGGCATAGGCATCTGGTTAAGCTAATGGGTTACTGCAGCAGCAAGTCAGAAGGATTGAATCTGTTGATATATGAGTACATGAAGAACGGAAGCATCTGGGATTGGCTTCATGAGGACAAACCCGTGCTCGAAAAAAAGAAGAAGCTTTTAGACTGGGAAGCAAGATTGAGAATAGCAGTAGGATTGGCTCAAGGAGTAGAGTACCTTCATCATGACTGTGTTCCTCCTATCGTTCACCGTGATATCAAATCCAGTAATGTGCTCCTCGATTCAAACATGGAAGCGCATTTAGGAGATTTCGGTCTTGCCAAGGTCTTAACTGAGAACTGTGATACCAACACAGATTCAAATACCTGGTTTGCATGCTCTTATGGCTACATTGCTCCAGAGTATGCTTACTCGTTGAAGGCGACTGAGAAGAGCGACGTTTACAGTATGGGGATCGTGTTGATGGAGATTGTGACTGGGAAAATGCCAACCGACTCAGTGTTTGGTGCAGAGATGGACATGGTGAGATGGGTTGAAACACATCTTGAAGTAGCGGGTTCTGCGAGAGATAAACTCATAGATCCAAAGCTTAAGCCACTTCTGCCATTTGAGGAAGACGCAGCTTGTCAGGTGCTTGAGATTGCACTTCAGTGCACAAAAACTTCTCCCCAAGAGAGACCATCTTCTAGGCAAGCTTGTGATAGTCTTCTCCATGTCTACAACAACAGAACGGCCGGTTATAAGAAGCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001704 GO:0001706 GO:0003006 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006810 GO:0006833 GO:0006950 GO:0007275 GO:0007369 GO:0007389 GO:0007492 GO:0008104 GO:0008150 GO:0009505 GO:0009531 GO:0009553 GO:0009611 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009798 GO:0009888 GO:0009987 GO:0010154 GO:0010453 GO:0010470 GO:0016020 GO:0022414 GO:0022603 GO:0030104 GO:0030154 GO:0030312 GO:0032501 GO:0032502 GO:0033036 GO:0035987 GO:0042044 GO:0042592 GO:0042659 GO:0044426 GO:0044462 GO:0044464 GO:0045184 GO:0045595 GO:0045995 GO:0048226 GO:0048229 GO:0048316 GO:0048598 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051239 GO:0051302 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0061458 GO:0065001 GO:0065007 GO:0065008 GO:0071944 GO:0090558 GO:0090708 GO:0098771 GO:0099402 GO:1903224 GO:1905392 GO:1905421 GO:2000026 GO:2000067 GO:2000280

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1249

Amino Acids

137.29

Weight (kDa)

5.46

Isoelectric Point (pI)

36.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	24 - 66	4.7e-12	Leucine rich repeat N-terminal domain
LRR_8	PF13855	90 - 131	3.1e-06	Leucine rich repeat
LRR_14	PF23598	137 - 247	3.6e-08	Leucine-rich repeat region
LRR_14	PF23598	257 - 383	9.2e-11	Leucine-rich repeat region
LRR_8	PF13855	264 - 323	1.3e-09	Leucine rich repeat
LRR_8	PF13855	337 - 396	8.6e-06	Leucine rich repeat
LRR_14	PF23598	354 - 464	2.3e-09	Leucine-rich repeat region
LRR_14	PF23598	471 - 560	2.4e-06	Leucine-rich repeat region
LRR_8	PF13855	505 - 564	1.3e-07	Leucine rich repeat
LRR_14	PF23598	693 - 771	1.1e-07	Leucine-rich repeat region
LRR_8	PF13855	773 - 828	7.9e-10	Leucine rich repeat
Pkinase	PF00069	956 - 1233	1.1e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	956 - 1231	1.2e-42	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014415)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20140
fragaria_vesca	FvH4_3g13270 FvH4_3g13270 FvH4_3g13270
malus_domestica	MD05G1243600.v1.1 MD10G1225500.v1.1
prunus_persica	Prupe.4G120100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0021741
rosa_laevigata	RLG00000032624
rosa_multiflora	Rmu_ssc0000233.1_g000008
rosa_roxburghii	Rroxscaffold_1G00056980
rosa_rugosa	Rorug05G0068900
rosa_samantha	Rh5AG157400 Rh5BG157100 Rh5CG171200 Rh5DG157300
rosa_wichuraiana	Rw5G014030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3738
AasI	GACNNNNNNGTC	1 cut(s)	540
AccB7I	CCANNNNNTGG	2 cut(s)	1715, 2000
AccI	GTMKAC	2 cut(s)	532, 3714
AccII	CGCG	1 cut(s)	1869
AciI	CCGC	9 cut(s)	665, 668, 698, 1491, 1535, 1867, 1919, 2903, 3551
AclWI	GGATC	8 cut(s)	128, 455, 1120, 1561, 2310, 2881, 3458, 3572
AcoI	YGGCCR	3 cut(s)	274, 2516, 3729
AcsI	RAATTY	2 cut(s)	917, 2113
AcuI	CTGAAG	6 cut(s)	65, 933, 1152, 2282, 2962, 3629
AcyI	GRCGYC	1 cut(s)	709
AfaI	GTAC	6 cut(s)	1195, 1699, 2083, 2744, 3086, 3212
AfiI	CCNNNNNNNGG	6 cut(s)	272, 1427, 1715, 1755, 2000, 2817
AflII	CTTAAG	2 cut(s)	1085, 3587
AgeI	ACCGGT	4 cut(s)	203, 236, 251, 539
AhlI	ACTAGT	1 cut(s)	10
AjnI	CCWGG	4 cut(s)	735, 1776, 2425, 3362
AjuI	GAANNNNNNNTTGG	4 cut(s)	92, 124, 1831, 1863
AleI	CACNNNNGTG	1 cut(s)	3646
Alw21I	GWGCWC	6 cut(s)	1727, 2028, 2161, 3138, 3273, 3653
Alw44I	GTGCAC	1 cut(s)	3649
AlwI	GGATC	8 cut(s)	128, 455, 1120, 1561, 2310, 2881, 3458, 3572
Ama87I	CYCGRG	2 cut(s)	92, 1549
AoxI	GGCC	3 cut(s)	274, 2516, 3729
ApaLI	GTGCAC	1 cut(s)	3649
ApeKI	GCWGC	6 cut(s)	1090, 2089, 2571, 3045, 3048, 3617
ApoI	RAATTY	2 cut(s)	917, 2113
ArsI	GACNNNNNNTTYG	2 cut(s)	3482, 3514
AsiGI	ACCGGT	4 cut(s)	203, 236, 251, 539
Asp700I	GAANNNNTTC	4 cut(s)	1328, 1400, 2008, 2504
AspLEI	GCGC	2 cut(s)	1289, 3295
AspS9I	GGNCC	5 cut(s)	180, 326, 542, 614, 1982
AsuC2I	CCSGG	2 cut(s)	60, 479
AsuII	TTCGAA	3 cut(s)	1404, 2039, 2791
AvaI	CYCGRG	2 cut(s)	92, 1549
AvaII	GGWCC	5 cut(s)	180, 326, 542, 614, 1982
BaeGI	GKGCMC	1 cut(s)	3653
BaeI	ACNNNNGTAYC	2 cut(s)	782, 815
BalI	TGGCCA	1 cut(s)	2518
BanII	GRGCYC	3 cut(s)	1727, 2028, 2161
BarI	GAAGNNNNNNTAC	6 cut(s)	1254, 1286, 1398, 1430, 3198, 3230
BbsI	GAAGAC	3 cut(s)	135, 3618, 3695
Bbv12I	GWGCWC	6 cut(s)	1727, 2028, 2161, 3138, 3273, 3653
BbvCI	CCTCAGC	1 cut(s)	2324
BbvI	GCAGC	6 cut(s)	1102, 2076, 2558, 3057, 3060, 3629
BccI	CCATC	8 cut(s)	338, 805, 2102, 2508, 3454, 3505, 3519, 3685
BceAI	ACGGC	2 cut(s)	2670, 3744
BciT130I	CCWGG	4 cut(s)	737, 1778, 2427, 3364
BcnI	CCSGG	2 cut(s)	60, 479
BcuI	ACTAGT	1 cut(s)	10
BfaI	CTAG	6 cut(s)	11, 320, 953, 1148, 1784, 3684
BfmI	CTRYAG	3 cut(s)	1229, 1922, 3043
BfrI	CTTAAG	2 cut(s)	1085, 3587
BglI	GCCNNNNNGGC	1 cut(s)	417
BglII	AGATCT	1 cut(s)	1162
BisI	GCNGC	7 cut(s)	666, 1091, 2090, 2572, 3046, 3049, 3618
BlpI	GCTNAGC	1 cut(s)	2225
BlsI	GCNGC	7 cut(s)	667, 1092, 2091, 2573, 3047, 3050, 3619
Bme1390I	CCNGG	6 cut(s)	60, 479, 737, 1778, 2427, 3364
Bme18I	GGWCC	5 cut(s)	180, 326, 542, 614, 1982
BmeT110I	CYCGRG	2 cut(s)	92, 1549
BmgT120I	GGNCC	5 cut(s)	180, 326, 542, 614, 1982
BmiI	GGNNCC	4 cut(s)	328, 421, 1658, 1912
BmrFI	CCNGG	6 cut(s)	60, 479, 737, 1778, 2427, 3364
BmrI	ACTGGG	3 cut(s)	2273, 3172, 3483
BmsI	GCATC	4 cut(s)	501, 1590, 3028, 3111
BmuI	ACTGGG	3 cut(s)	2273, 3172, 3483
BoxI	GACNNNNGTC	1 cut(s)	185
BpiI	GAAGAC	3 cut(s)	135, 3618, 3695
BplI	GAGNNNNNCTC	4 cut(s)	2478, 2510, 3555, 3587
BpmI	CTGGAG	5 cut(s)	491, 779, 1799, 2364, 3378
Bpu10I	CCTNAGC	2 cut(s)	443, 2324
Bpu1102I	GCTNAGC	1 cut(s)	2225
Bpu14I	TTCGAA	3 cut(s)	1404, 2039, 2791
BpuEI	CTTGAG	7 cut(s)	1480, 2012, 2193, 2480, 2494, 3183, 3653
BpuMI	CCSGG	2 cut(s)	60, 479
Bsa29I	ATCGAT	1 cut(s)	1956
BsaBI	GATNNNNATC	2 cut(s)	603, 1167
BsaHI	GRCGYC	1 cut(s)	709
BsaI	GGTCTC	1 cut(s)	3667
BsaJI	CCNNGG	8 cut(s)	266, 360, 1185, 1776, 1847, 1994, 2482, 3318
BsaWI	WCCGGW	9 cut(s)	44, 182, 203, 236, 251, 435, 539, 755, 899
BsaXI	ACNNNNNCTCC	6 cut(s)	356, 386, 1181, 1186, 1211, 1216
Bsc4I	CCNNNNNNNGG	6 cut(s)	272, 1427, 1715, 1755, 2000, 2817
Bse118I	RCCGGY	8 cut(s)	203, 236, 251, 276, 395, 539, 694, 3731
Bse1I	ACTGG	9 cut(s)	796, 1026, 1494, 2188, 2279, 2347, 3167, 3261, 3478
Bse3DI	GCAATG	1 cut(s)	3387
Bse8I	GATNNNNATC	2 cut(s)	603, 1167
BseBI	CCWGG	4 cut(s)	737, 1778, 2427, 3364
BseCI	ATCGAT	1 cut(s)	1956
BseDI	CCNNGG	8 cut(s)	266, 360, 1185, 1776, 1847, 1994, 2482, 3318
BseGI	GGATG	5 cut(s)	30, 330, 1565, 1705, 2959
BseJI	GATNNNNATC	2 cut(s)	603, 1167
BseLI	CCNNNNNNNGG	6 cut(s)	272, 1427, 1715, 1755, 2000, 2817
BseMI	GCAATG	1 cut(s)	3387
BseNI	ACTGG	9 cut(s)	796, 1026, 1494, 2188, 2279, 2347, 3167, 3261, 3478
BseRI	GAGGAG	4 cut(s)	11, 14, 2873, 3263
BseSI	GKGCMC	1 cut(s)	3653
BseX3I	CGGCCG	1 cut(s)	3729
BseXI	GCAGC	6 cut(s)	1102, 2076, 2558, 3057, 3060, 3629
BsgI	GTGCAG	2 cut(s)	1348, 3525
Bsh1236I	CGCG	1 cut(s)	1869
Bsh1285I	CGRYCG	3 cut(s)	440, 565, 3732
BshFI	GGCC	3 cut(s)	276, 2518, 3731
BshTI	ACCGGT	4 cut(s)	203, 236, 251, 539
BshVI	ATCGAT	1 cut(s)	1956
BsiEI	CGRYCG	3 cut(s)	440, 565, 3732
BsiHKAI	GWGCWC	6 cut(s)	1727, 2028, 2161, 3138, 3273, 3653
BsiHKCI	CYCGRG	2 cut(s)	92, 1549
BslFI	GGGAC	3 cut(s)	312, 700, 2380
BslI	CCNNNNNNNGG	6 cut(s)	272, 1427, 1715, 1755, 2000, 2817
BsmBI	CGTCTC	3 cut(s)	476, 572, 1814
BsmFI	GGGAC	3 cut(s)	312, 700, 2380
BsnI	GGCC	3 cut(s)	276, 2518, 3731
Bso31I	GGTCTC	1 cut(s)	3667
BsoBI	CYCGRG	2 cut(s)	92, 1549
Bsp119I	TTCGAA	3 cut(s)	1404, 2039, 2791
Bsp1286I	GDGCHC	6 cut(s)	1727, 2028, 2161, 3138, 3273, 3653
Bsp1407I	TGTACA	1 cut(s)	2081
Bsp1720I	GCTNAGC	1 cut(s)	2225
Bsp19I	CCATGG	1 cut(s)	1994
BspACI	CCGC	9 cut(s)	665, 668, 698, 1491, 1535, 1867, 1919, 2903, 3551
BspANI	GGCC	3 cut(s)	276, 2518, 3731
BspDI	ATCGAT	1 cut(s)	1956
BspFNI	CGCG	1 cut(s)	1869
BspHI	TCATGA	3 cut(s)	2865, 3118, 3220
BspLI	GGNNCC	4 cut(s)	328, 421, 1658, 1912
BspMAI	CTGCAG	1 cut(s)	3047
BspPI	GGATC	8 cut(s)	128, 455, 1120, 1561, 2310, 2881, 3458, 3572
BspQI	GCTCTTC	2 cut(s)	2764, 3422
BspT104I	TTCGAA	3 cut(s)	1404, 2039, 2791
BspTI	CTTAAG	2 cut(s)	1085, 3587
BspTNI	GGTCTC	1 cut(s)	3667
BsrDI	GCAATG	1 cut(s)	3387
BsrFI	RCCGGY	8 cut(s)	203, 236, 251, 276, 395, 539, 694, 3731
BsrGI	TGTACA	1 cut(s)	2081
BsrI	ACTGG	9 cut(s)	796, 1026, 1494, 2188, 2279, 2347, 3167, 3261, 3478
BssAI	RCCGGY	8 cut(s)	203, 236, 251, 276, 395, 539, 694, 3731
BssECI	CCNNGG	8 cut(s)	266, 360, 1185, 1776, 1847, 1994, 2482, 3318
BssNI	GRCGYC	1 cut(s)	709
BssT1I	CCWWGG	6 cut(s)	266, 360, 1185, 1847, 1994, 3318
Bst2UI	CCWGG	4 cut(s)	737, 1778, 2427, 3364
Bst6I	CTCTTC	7 cut(s)	32, 93, 1623, 2441, 2693, 2764, 3422
BstACI	GRCGYC	1 cut(s)	709
BstAFI	CTTAAG	2 cut(s)	1085, 3587
BstAUI	TGTACA	1 cut(s)	2081
BstBI	TTCGAA	3 cut(s)	1404, 2039, 2791
BstC8I	GCNNGC	8 cut(s)	413, 516, 1120, 1871, 2231, 2520, 3373, 3690
BstDSI	CCRYGG	1 cut(s)	1994
BstENI	CCTNNNNNAGG	1 cut(s)	1425
BstF5I	GGATG	5 cut(s)	30, 330, 1565, 1705, 2959
BstFNI	CGCG	1 cut(s)	1869
BstHHI	GCGC	2 cut(s)	1289, 3295
BstMCI	CGRYCG	3 cut(s)	440, 565, 3732
BstNI	CCWGG	4 cut(s)	737, 1778, 2427, 3364
BstNSI	RCATGY	3 cut(s)	195, 679, 3375
BstPAI	GACNNNNGTC	1 cut(s)	185
BstSCI	CCNGG	6 cut(s)	58, 477, 735, 1776, 2425, 3362
BstSFI	CTRYAG	3 cut(s)	1229, 1922, 3043
BstSLI	GKGCMC	1 cut(s)	3653
BstUI	CGCG	1 cut(s)	1869
BstV1I	GCAGC	6 cut(s)	1102, 2076, 2558, 3057, 3060, 3629
BstV2I	GAAGAC	3 cut(s)	135, 3618, 3695
BstX2I	RGATCY	3 cut(s)	1162, 2302, 3577
BstXI	CCANNNNNNTGG	1 cut(s)	847
BstYI	RGATCY	3 cut(s)	1162, 2302, 3577
BstZI	CGGCCG	1 cut(s)	3729
Bsu15I	ATCGAT	1 cut(s)	1956
BsuRI	GGCC	3 cut(s)	276, 2518, 3731
BsuTUI	ATCGAT	1 cut(s)	1956
BtgI	CCRYGG	1 cut(s)	1994
BtsCI	GGATG	5 cut(s)	30, 330, 1565, 1705, 2959
BtsI	GCAGTG	3 cut(s)	660, 2736, 2937
BtsIMutI	CAGTG	8 cut(s)	651, 660, 1238, 2340, 2736, 2937, 3501, 3653
Cac8I	GCNNGC	8 cut(s)	413, 516, 1120, 1871, 2231, 2520, 3373, 3690
CciI	TCATGA	3 cut(s)	2865, 3118, 3220
CfoI	GCGC	2 cut(s)	1289, 3295
Cfr10I	RCCGGY	8 cut(s)	203, 236, 251, 276, 395, 539, 694, 3731
Cfr13I	GGNCC	5 cut(s)	180, 326, 542, 614, 1982
ClaI	ATCGAT	1 cut(s)	1956
CseI	GACGC	3 cut(s)	717, 3004, 3623
CsiI	ACCWGGT	1 cut(s)	3362
Csp6I	GTAC	6 cut(s)	1194, 1698, 2082, 2743, 3085, 3211
CspAI	ACCGGT	4 cut(s)	203, 236, 251, 539
CviQI	GTAC	6 cut(s)	1194, 1698, 2082, 2743, 3085, 3211
DrdI	GACNNNNNNGTC	1 cut(s)	540
DseDI	GACNNNNNNGTC	1 cut(s)	540
EaeI	YGGCCR	3 cut(s)	274, 2516, 3729
EagI	CGGCCG	1 cut(s)	3729
Eam1104I	CTCTTC	7 cut(s)	32, 93, 1623, 2441, 2693, 2764, 3422
EarI	CTCTTC	7 cut(s)	32, 93, 1623, 2441, 2693, 2764, 3422
EciI	GGCGGA	4 cut(s)	683, 713, 1550, 2918
Ecl136II	GAGCTC	3 cut(s)	1725, 2026, 2159
EclXI	CGGCCG	1 cut(s)	3729
Eco130I	CCWWGG	6 cut(s)	266, 360, 1185, 1847, 1994, 3318
Eco24I	GRGCYC	3 cut(s)	1727, 2028, 2161
Eco31I	GGTCTC	1 cut(s)	3667
Eco32I	GATATC	5 cut(s)	475, 1212, 2459, 2815, 3253
Eco47I	GGWCC	5 cut(s)	180, 326, 542, 614, 1982
Eco52I	CGGCCG	1 cut(s)	3729
Eco53kI	GAGCTC	3 cut(s)	1725, 2026, 2159
Eco57I	CTGAAG	6 cut(s)	65, 933, 1152, 2282, 2962, 3629
Eco88I	CYCGRG	2 cut(s)	92, 1549
EcoICRI	GAGCTC	3 cut(s)	1725, 2026, 2159
EcoNI	CCTNNNNNAGG	1 cut(s)	1425
EcoO109I	RGGNCCY	1 cut(s)	1982
EcoRI	GAATTC	1 cut(s)	917
EcoRII	CCWGG	4 cut(s)	735, 1776, 2425, 3362
EcoRV	GATATC	5 cut(s)	475, 1212, 2459, 2815, 3253
EcoT14I	CCWWGG	6 cut(s)	266, 360, 1185, 1847, 1994, 3318
EcoT38I	GRGCYC	3 cut(s)	1727, 2028, 2161
ErhI	CCWWGG	6 cut(s)	266, 360, 1185, 1847, 1994, 3318
Esp3I	CGTCTC	3 cut(s)	476, 572, 1814
FalI	AAGNNNNNCTT	6 cut(s)	1614, 1646, 2746, 2778, 2771, 2803
FaqI	GGGAC	3 cut(s)	312, 700, 2380
FauI	CCCGC	2 cut(s)	1498, 3544
FblI	GTMKAC	2 cut(s)	532, 3714
Fnu4HI	GCNGC	7 cut(s)	666, 1091, 2090, 2572, 3046, 3049, 3618
FokI	GGATG	5 cut(s)	17, 317, 1552, 1692, 2966
FriOI	GRGCYC	3 cut(s)	1727, 2028, 2161
Fsp4HI	GCNGC	7 cut(s)	666, 1091, 2090, 2572, 3046, 3049, 3618
FspBI	CTAG	6 cut(s)	11, 320, 953, 1148, 1784, 3684
GlaI	GCGC	2 cut(s)	1288, 3294
GluI	GCNGC	7 cut(s)	666, 1091, 2090, 2572, 3046, 3049, 3618
GsuI	CTGGAG	5 cut(s)	491, 779, 1799, 2364, 3378
HaeIII	GGCC	3 cut(s)	276, 2518, 3731
HgaI	GACGC	3 cut(s)	717, 3004, 3623
HhaI	GCGC	2 cut(s)	1289, 3295
Hin1I	GRCGYC	1 cut(s)	709
Hin6I	GCGC	2 cut(s)	1287, 3293
HinP1I	GCGC	2 cut(s)	1287, 3293
HincII	GTYRAC	1 cut(s)	56
HindII	GTYRAC	1 cut(s)	56
HindIII	AAGCTT	5 cut(s)	1369, 2030, 3152, 3584, 3690
Hpy99I	CGWCG	1 cut(s)	3437
HpyCH4IV	ACGT	4 cut(s)	485, 1196, 1853, 3435
HpySE526I	ACGT	4 cut(s)	485, 1196, 1853, 3435
Hsp92I	GRCGYC	1 cut(s)	709
HspAI	GCGC	2 cut(s)	1287, 3293
LguI	GCTCTTC	2 cut(s)	2764, 3422
LmnI	GCTCC	9 cut(s)	425, 700, 1015, 1780, 2156, 2798, 2905, 3276, 3397
Lsp1109I	GCAGC	6 cut(s)	1102, 2076, 2558, 3057, 3060, 3629
LweI	GCATC	4 cut(s)	501, 1590, 3028, 3111
MabI	ACCWGGT	1 cut(s)	3362
MaeI	CTAG	6 cut(s)	11, 320, 953, 1148, 1784, 3684
MaeII	ACGT	4 cut(s)	485, 1196, 1853, 3435
MaeIII	GTNAC	8 cut(s)	112, 187, 1269, 1502, 1744, 2147, 3038, 3469
MfeI	CAATTG	2 cut(s)	1829, 2361
MflI	RGATCY	3 cut(s)	1162, 2302, 3577
MhlI	GDGCHC	6 cut(s)	1727, 2028, 2161, 3138, 3273, 3653
MlsI	TGGCCA	1 cut(s)	2518
MluNI	TGGCCA	1 cut(s)	2518
MlyI	GAGTC	9 cut(s)	79, 195, 374, 528, 562, 778, 940, 2063, 3485
MmeI	TCCRAC	4 cut(s)	166, 336, 372, 448
Mox20I	TGGCCA	1 cut(s)	2518
MroXI	GAANNNNTTC	4 cut(s)	1328, 1400, 2008, 2504
MscI	TGGCCA	1 cut(s)	2518
MslI	CAYNNNNRTG	2 cut(s)	3225, 3646
Msp20I	TGGCCA	1 cut(s)	2518
MspA1I	CMGCKG	1 cut(s)	2522
MspCI	CTTAAG	2 cut(s)	1085, 3587
MspR9I	CCNGG	6 cut(s)	60, 479, 737, 1778, 2427, 3364
MunI	CAATTG	2 cut(s)	1829, 2361
MvaI	CCWGG	4 cut(s)	737, 1778, 2427, 3364
MvnI	CGCG	1 cut(s)	1869
NciI	CCSGG	2 cut(s)	60, 479
NcoI	CCATGG	1 cut(s)	1994
NlaIV	GGNNCC	4 cut(s)	328, 421, 1658, 1912
NmeAIII	GCCGAG	1 cut(s)	396
NmuCI	GTSAC	4 cut(s)	112, 187, 1744, 3469
NspI	RCATGY	3 cut(s)	195, 679, 3375
NspV	TTCGAA	3 cut(s)	1404, 2039, 2791
OliI	CACNNNNGTG	1 cut(s)	3646
PaeI	GCATGC	1 cut(s)	3375
PaeR7I	CTCGAG	1 cut(s)	92
PagI	TCATGA	3 cut(s)	2865, 3118, 3220
PciSI	GCTCTTC	2 cut(s)	2764, 3422
PcsI	WCGNNNNNNNCGW	4 cut(s)	423, 567, 571, 3416
PdmI	GAANNNNTTC	4 cut(s)	1328, 1400, 2008, 2504
PflMI	CCANNNNNTGG	2 cut(s)	1715, 2000
PfoI	TCCNGGA	1 cut(s)	477
PinAI	ACCGGT	4 cut(s)	203, 236, 251, 539
PkrI	GCNGC	7 cut(s)	667, 1092, 2091, 2573, 3047, 3050, 3619
PleI	GAGTC	9 cut(s)	79, 194, 373, 528, 561, 777, 939, 2062, 3485
PpsI	GAGTC	9 cut(s)	79, 194, 373, 528, 561, 777, 939, 2062, 3485
PpuMI	RGGWCCY	1 cut(s)	1982
PshAI	GACNNNNGTC	1 cut(s)	185
PsiI	TTATAA	1 cut(s)	3738
Psp124BI	GAGCTC	3 cut(s)	1727, 2028, 2161
Psp5II	RGGWCCY	1 cut(s)	1982
Psp6I	CCWGG	4 cut(s)	735, 1776, 2425, 3362
PspGI	CCWGG	4 cut(s)	735, 1776, 2425, 3362
PspN4I	GGNNCC	4 cut(s)	328, 421, 1658, 1912
PspPI	GGNCC	5 cut(s)	180, 326, 542, 614, 1982
PspPPI	RGGWCCY	1 cut(s)	1982
PstI	CTGCAG	1 cut(s)	3047
PsuI	RGATCY	3 cut(s)	1162, 2302, 3577
PvuII	CAGCTG	1 cut(s)	2522
RsaI	GTAC	6 cut(s)	1195, 1699, 2083, 2744, 3086, 3212
RsaNI	GTAC	6 cut(s)	1194, 1698, 2082, 2743, 3085, 3211
RseI	CAYNNNNRTG	2 cut(s)	3225, 3646
SacI	GAGCTC	3 cut(s)	1727, 2028, 2161
SapI	GCTCTTC	2 cut(s)	2764, 3422
SatI	GCNGC	7 cut(s)	666, 1091, 2090, 2572, 3046, 3049, 3618
Sau96I	GGNCC	5 cut(s)	180, 326, 542, 614, 1982
SchI	GAGTC	9 cut(s)	79, 195, 374, 528, 562, 778, 940, 2063, 3485
ScrFI	CCNGG	6 cut(s)	60, 479, 737, 1778, 2427, 3364
SduI	GDGCHC	6 cut(s)	1727, 2028, 2161, 3138, 3273, 3653
SexAI	ACCWGGT	1 cut(s)	3362
SfaNI	GCATC	4 cut(s)	501, 1590, 3028, 3111
SfcI	CTRYAG	3 cut(s)	1229, 1922, 3043
Sfr274I	CTCGAG	1 cut(s)	92
SfuI	TTCGAA	3 cut(s)	1404, 2039, 2791
SgrAI	CRCCGGYG	1 cut(s)	395
SinI	GGWCC	5 cut(s)	180, 326, 542, 614, 1982
SlaI	CTCGAG	1 cut(s)	92
SmiMI	CAYNNNNRTG	2 cut(s)	3225, 3646
SpeI	ACTAGT	1 cut(s)	10
SphI	GCATGC	1 cut(s)	3375
SsiI	CCGC	9 cut(s)	665, 668, 698, 1491, 1535, 1867, 1919, 2903, 3551
SspMI	CTAG	6 cut(s)	11, 320, 953, 1148, 1784, 3684
SstI	GAGCTC	3 cut(s)	1727, 2028, 2161
StyD4I	CCNGG	6 cut(s)	58, 477, 735, 1776, 2425, 3362
StyI	CCWWGG	6 cut(s)	266, 360, 1185, 1847, 1994, 3318
TaiI	ACGT	4 cut(s)	488, 1199, 1856, 3438
TaqII	GACCGA	3 cut(s)	551, 631, 3299
TatI	WGTACW	2 cut(s)	2081, 3084
TauI	GCSGC	1 cut(s)	668
TscAI	CASTG	8 cut(s)	658, 667, 1238, 2347, 2743, 2937, 3501, 3653
TseFI	GTSAC	4 cut(s)	112, 187, 1744, 3469
TseI	GCWGC	6 cut(s)	1090, 2089, 2571, 3045, 3048, 3617
Tsp45I	GTSAC	4 cut(s)	112, 187, 1744, 3469
TspGWI	ACGGA	2 cut(s)	2811, 3111
TspRI	CASTG	8 cut(s)	658, 667, 1238, 2347, 2743, 2937, 3501, 3653
Van91I	CCANNNNNTGG	2 cut(s)	1715, 2000
Vha464I	CTTAAG	2 cut(s)	1085, 3587
VneI	GTGCAC	1 cut(s)	3649
VpaK11BI	GGWCC	5 cut(s)	180, 326, 542, 614, 1982
XagI	CCTNNNNNAGG	1 cut(s)	1425
XapI	RAATTY	2 cut(s)	917, 2113
XceI	RCATGY	3 cut(s)	195, 679, 3375
XhoI	CTCGAG	1 cut(s)	92
XmiI	GTMKAC	2 cut(s)	532, 3714
XmnI	GAANNNNTTC	4 cut(s)	1328, 1400, 2008, 2504
XspI	CTAG	6 cut(s)	11, 320, 953, 1148, 1784, 3684

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.