Rroxscaffold_1G00056980
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014415
Physical Location & Seq
Reverse (-)
78950354 .. 78954644
4291 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00056980.1

Sequence Viewer

Length: 3777 bp
ATGGCTGCAACAACAACAAAGATGTCAATTTTACCACTTTTTCTCATTCTTCTTGTCATTTCTTCTATTTTTGTGGAGGCACAGCTCGATAGCAATACGACTCTAAAAGTGCTTTTGGAGGTGAAGAAGTCGTTTTTGGAGGACCCAATATCCGAAACAGCCCTGCATGGATGGTCTGAAAGCAACCCAAATTTCTGTACTTGGAGAGGAGTGACATGCGGGTCTGACTCGGTCGGTGACTCGGTCCAAGTGGTCGGACTCAACCTTTCCGACTCGTCACTCGGCGGGTCCATATCTCCCCAACTCGGTAGTTTGAAGTACCTGCTCCACCTCGATCTCTCTTCTAACGGCCTCACGGGTCCCATCCCACCAGCCCTCTCAAACCTCTCTTCCTTGGAGTCTTTGCTCCTCTTTACGAACCGACTCAGTGGACCCATCCCGAGTGAACTCGGCTCGCTCACGAGTCTCCGGGTCATGAGAATCGGTGACAATGAGCTAACCGGGTCCATTCCAGCCTCATTTGGTAACCTTGTCAATTTGATCACCCTTGCGGTGGCGTCATGTGGTCTCAACGGTCCAATACCACCCCAACTCGGCCGGCTCGGTCAGCTGGAGAATTTAATCATCCAGCAGAACCAACTCGAGGGTCGGATTCCGGCCGAGTTGGGAAACTGTTCTAGTCTCACTGTGTTCACGGCTGCTGAAAATAAACTCAACGGCTCGATTCCCGGGGAATTGGGTCAGCTCACCAATCTCCAGCTACTCAACTTGGTCAACAACAGCCTCTCCGGGGAGATACCGAGTCAACTCGGTGGGATGAGTCAACTCGGGTACCTTAACTTTATGGGGAACCAACTTGAGGGTGCAATACCCAAGTCCTTGGCTCAATTGGGCAATCTTCAGAACCTTGATTTGTCCATTAACAAGCTCACAGGAGGAATCCCAGAGGAACTAGGTAACATGGGTCAACTGGTTTACTTGGTTTTGTCCAACAACAATCTCTCTGGTGTTATACCAAAGACTATATGCTCCAATGCAACTAGTTTGGAGCACTTGGTGATATCTGATTCTCAAATTTTCGGCGAAATACCAGCTGAGCTGAGCCAGTGTCAATCCCTGAAGCAACTTGACTTGTCCAACAATTCAATCAGTGGATCAATTCCACTCAAACTGTATGGCTTGGTGGAGTTAACTGACCTCTTGCTCCACAACAACAGCTTGGTTGGTTCAATTTCTCCTTATATAGGCAACCTCAGCAATCTTCAGACTCTTGTTTTGTATCACAACAATTTGCAGGGTCCTCTGCCTAAAGAGATTGGTATGCTTGGGCAGCTAGAGATTTTGTATCTCTATGATAACCAGCTTAGTGGTGAAATCCCACTTGAGATTGGGAATTGTTCGAGTTTGCAGATGATTGACTTCTTTGGGAACAAGTTCAGTGGGGAGATCCCAATCACTATTGGGAGGCTTAAAGATTTGAACTTTCTTCATATCAGGCAGAATGAGCTAGTGGGTGAGCTTCCTGCCACATTGGGCAACTGTCGTCAACTAACTATCCTGGACTTGGCGGATAACCGTTTGTCTGGTGGCATTCCTATTACATTTGGGTCTCTTCAAGCAATGCAGCAGTTCATGCTATACAACAATTCACTTGAAGGTAATCTTCCTGAAACATTGGTTCATATGGCGAATTTGACTAGAGTGAATTTGTCGAAAAACAAATTGAATGGAAGCATTGCTGCATTATGTAGCTCGAATTCATTCCTTTCTTTTGATGTCACTGATAATGCCTTTGATGGTGAGATTCCTCCCCATCTGGGAAATTCAACCTCCCTTGAGAGGTTGAGATTGGGGAACAACCAATTTACGGGTCCAATCCCAGTTACATTGGGAAAAATCAGTGAGCTATCACTGTTAGATGTGTCAGGGAATTCACTCACTGGACCAATCCCAGTTGAGCTTTCATCGTGCAAGAAATTGTCTCATTTTGATTTGAATGACAACCTTCTTTCTGGCATCATTCCCATGTGGCTTGGAAGTTTGCCTCAGCTAGGAGAGTTGAAGCTCTCCTCAAATCGATTCACAGGGCCACTTCCCCAAGAGTTGTTCAATTGTTCTAAACTGCTAGTGCTTTCTTTGAATGACAATTTGCTCAATGGAACCCTCCCTGCTGAGATTGGCAACCTGGGTTCTCTCAATGTCCTAAACCTCGACCACAACCAATTCTCGGGACCAATGCCCCCAGCCATTGGTAAGCTAGGCAACCTCTATGAGCTTAGGCTCTCCCAAAACAGCTTTGATGGTAACATACCCTATGAGCTTGGACAACTCCAAAATCTTCAGAGCATGTTAGACCTCAGTTCCAATAAACTGAATGGTCAGATCCCGGCATCTATTGGGACACTATCAAAACTTGAGGCGCTTAATCTATCTCACAATCAACTTAGTGGAGAAGTCCCCTCTACAATTGGTGCGATGAGTAGCTTGGGAAAGCTTAATCTCACTTACAACAATCTGGAAGGAAAATTGAGCAAGCAACTCTCACACTGGCCAGCCGAGGCCTTTGCAGGAAATTTACATCTTTGTGGGAACCCTCTTGGTAGCTGCAATGGCAGCAAGAACCAGTCAGGCCCAAATGAATTGACTGTGATAGTCATTTCTGCATTATTTACTTTAGCTGCAATTGCCCTGCTAATATTTGGAGCTGCTTCTTTCTTGAAACACAGGCGAGAAGCGCTTAGAAAAGCCAGTGAAGTGAACTGCGTGTACTCATCGAGTTCTTCTCATGCTAAGCGGAGACTGCTATTTTCAAATGGTTCTGTCAAGCCAGATTTCAAGTGGAAAGACATCATGGATTCCACCAACAATCTAAGTATAGAGTTTATGATCGGATCAGGAGGATCCGGAGTAGTCTACAGAGCTGAATTGTCTACCGGAGAAACTGTGGCAGTGAAGAAGATTTTGTACAAGGATGACCTTATGTCAAATAAAAGCTTCACAAGGGAGATTAAGACGCTTGGGAGGATAAGGCACAGGCATTTAGTGAAGCTAATGGGGTATTGTAGCAACAAAGGAGCAGGCTCCAATCTGTTGATCTATGAGTACATGGAAAACGGAAGCGTATGGGATTGGATACACAAAGAACAAGTTGACAGCAAGAAGAAGAGCCTTGACTGGGAAGCAAGGTTAAAGACTGCAGTTGGGTTAGCTCAAGGAGTGGAGTATCTCCACCATGACTGTGTGCCAAAGATCATTCACAGGGACATTAAAACAAGCAATGTCTTGCTAGACTCGAATATGGAGGCACATCTTGGGGATTTTGGCCTTGCCAAGACACTCAATGAGAATTTTGAGTCCAACACAGAATCAACTACATGGTTTGCTGGATCCTACGGTTACATAGCCCCAGAGTATGCATATTCGCTCAAGGCGACAGAAAAGAGTGATGTCTACAGCATGGGAATTGTGCTGATGGAGCTCGTCAGCGGAAAAATGCCGACGCATGCATCTTTTGGAGAGGAGATGGACATGGTGAGATGGGTGGAGACGCATATTGAAATGCAAGATCAAATTCGTGAGGAGTTGATAGACCCTGCACTGAGACCACTCTTATCCGGGGAAGAATATGCAGCATTCCAAGTACTAGAGATAGCACTGCAGTGCACAAAAACCAGCCCGGCTGAGAGGCCTTCATCACGGCAAGCCTGTGATCAGTTATTACATGTTTTCAATCATAGGATGGTGGAGTTTGATAAGACGAATGTAGATAGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1258

Amino Acids

136.51

Weight (kDa)

5.4

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 73 9.8e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 169 - 258 8.2e-07 Leucine-rich repeat region
LRR_14 PF23598 268 - 378 1.9e-07 Leucine-rich repeat region
LRR_14 PF23598 365 - 474 5.6e-09 Leucine-rich repeat region
LRR_14 PF23598 481 - 574 4.1e-07 Leucine-rich repeat region
LRR_8 PF13855 515 - 575 1.2e-07 Leucine rich repeat
LRR_14 PF23598 706 - 782 1.4e-06 Leucine-rich repeat region
LRR_14 PF23598 759 - 837 1e-06 Leucine-rich repeat region
LRR_8 PF13855 783 - 839 3.9e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 960 - 1236 5e-42 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 962 - 1238 7.1e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014415)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20140
fragaria_vesca FvH4_3g13270 FvH4_3g13270 FvH4_3g13270
malus_domestica MD05G1243600.v1.1 MD10G1225500.v1.1
prunus_persica Prupe.4G120100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0021741
rosa_laevigata RLG00000032624
rosa_multiflora Rmu_ssc0000233.1_g000008
rosa_roxburghii Rroxscaffold_1G00056980
rosa_rugosa Rorug05G0068900
rosa_samantha Rh5AG157400 Rh5BG157100 Rh5CG171200 Rh5DG157300
rosa_wichuraiana Rw5G014030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 220
Acc36I ACCTGC 1 cut(s) 330
Acc65I GGTACC 1 cut(s) 831
AccB1I GGYRCC 1 cut(s) 831
AccB7I CCANNNNNTGG 1 cut(s) 2249
AccI GTMKAC 3 cut(s) 2913, 2930, 3450
AccIII TCCGGA 1 cut(s) 2903
AciI CCGC 6 cut(s) 219, 285, 551, 1568, 2794, 3486
AclWI GGATC 8 cut(s) 1162, 1441, 2377, 2895, 2899, 2908, 3381, 3394
AcoI YGGCCR 3 cut(s) 595, 657, 2549
AcuI CTGAAG 4 cut(s) 884, 1139, 1247, 2324
AcyI GRCGYC 1 cut(s) 557
AdeI CACNNNGTG 1 cut(s) 1057
AfaI GTAC 7 cut(s) 199, 320, 833, 2768, 2966, 3104, 3642
AfeI AGCGCT 1 cut(s) 2736
AflIII ACRYGT 1 cut(s) 3721
AhdI GACNNNNNGTC 1 cut(s) 2980
AhlI ACTAGT 1 cut(s) 1040
AjnI CCWGG 2 cut(s) 1557, 2184
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AleI CACNNNNGTG 1 cut(s) 3658
Alw21I GWGCWC 3 cut(s) 1053, 3480, 3665
Alw26I GTCTC 8 cut(s) 470, 572, 686, 1614, 1986, 2791, 3539, 3595
Alw44I GTGCAC 1 cut(s) 3661
AlwI GGATC 8 cut(s) 1162, 1441, 2377, 2895, 2899, 2908, 3381, 3394
Ama87I CYCGRG 5 cut(s) 439, 641, 728, 827, 2227
Aor13HI TCCGGA 1 cut(s) 2903
Aor51HI AGCGCT 1 cut(s) 2736
AoxI GGCC 9 cut(s) 349, 595, 657, 2087, 2549, 2559, 2629, 3322, 3686
ApaLI GTGCAC 1 cut(s) 3661
ArsI GACNNNNNNTTYG 2 cut(s) 1258, 1290
Asp700I GAANNNNTTC 3 cut(s) 673, 1433, 1760
Asp718I GGTACC 1 cut(s) 831
AspLEI GCGC 2 cut(s) 2422, 2737
AsuC2I CCSGG 8 cut(s) 470, 502, 729, 730, 790, 2387, 3616, 3677
AvaI CYCGRG 5 cut(s) 439, 641, 728, 827, 2227
BaeGI GKGCMC 1 cut(s) 3665
BaeI ACNNNNGTAYC 2 cut(s) 815, 848
BalI TGGCCA 1 cut(s) 2551
BamHI GGATCC 2 cut(s) 2900, 3386
BanI GGYRCC 1 cut(s) 831
BanII GRGCYC 1 cut(s) 3480
BarI GAAGNNNNNNTAC 2 cut(s) 2948, 2980
BauI CACGAG 1 cut(s) 460
Bbv12I GWGCWC 3 cut(s) 1053, 3480, 3665
BbvCI CCTCAGC 2 cut(s) 1253, 2046
BbvI GCAGC 9 cut(s) 685, 1342, 1636, 1726, 2591, 2625, 2665, 2692, 3641
BceAI ACGGC 4 cut(s) 364, 711, 733, 3713
BcgI CGANNNNNNTGC 2 cut(s) 2546, 2580
BciT130I CCWGG 2 cut(s) 1559, 2186
BciVI GTATCC 1 cut(s) 3126
BclI TGATCA 2 cut(s) 540, 3709
BcnI CCSGG 8 cut(s) 470, 502, 729, 730, 790, 2387, 3616, 3677
BcoDI GTCTC 8 cut(s) 470, 572, 686, 1614, 1986, 2791, 3539, 3595
BcuI ACTAGT 1 cut(s) 1040
BfmI CTRYAG 4 cut(s) 2914, 3195, 3451, 3656
BfoI RGCGCY 2 cut(s) 2423, 2738
BfuAI ACCTGC 1 cut(s) 330
BfuI GTATCC 1 cut(s) 3126
BlpI GCTNAGC 3 cut(s) 1095, 1100, 2790
BmcAI AGTACT 1 cut(s) 3642
BmeRI GACNNNNNGTC 1 cut(s) 2980
BmeT110I CYCGRG 5 cut(s) 439, 641, 728, 827, 2227
BmrI ACTGGG 3 cut(s) 1874, 1946, 3184
BmsI GCATC 3 cut(s) 2025, 2399, 3515
BmuI ACTGGG 3 cut(s) 1874, 1946, 3184
BplI GAGNNNNNCTC 2 cut(s) 2767, 2799
BpmI CTGGAG 2 cut(s) 632, 740
Bpu10I CCTNAGC 3 cut(s) 1253, 2046, 2276
Bpu1102I GCTNAGC 3 cut(s) 1095, 1100, 2790
BpuEI CTTGAG 6 cut(s) 878, 1403, 1856, 2435, 3195, 3410
BpuMI CCSGG 8 cut(s) 470, 502, 729, 730, 790, 2387, 3616, 3677
Bsa29I ATCGAT 1 cut(s) 2077
BsaBI GATNNNNATC 1 cut(s) 1451
BsaHI GRCGYC 1 cut(s) 557
BsaI GGTCTC 3 cut(s) 572, 1614, 3595
BsaJI CCNNGG 8 cut(s) 393, 728, 729, 789, 879, 2185, 2556, 3615
BsaWI WCCGGW 2 cut(s) 2903, 2933
BsaXI ACNNNNNCTCC 4 cut(s) 770, 800, 2928, 2958
Bse118I RCCGGY 1 cut(s) 597
Bse1I ACTGG 9 cut(s) 975, 1105, 1880, 1945, 1952, 2552, 2623, 2748, 3179
Bse3DI GCAATG 4 cut(s) 1626, 1734, 2614, 3283
Bse8I GATNNNNATC 1 cut(s) 1451
BseAI TCCGGA 1 cut(s) 2903
BseBI CCWGG 2 cut(s) 1559, 2186
BseCI ATCGAT 1 cut(s) 2077
BseDI CCNNGG 8 cut(s) 393, 728, 729, 789, 879, 2185, 2556, 3615
BseGI GGATG 7 cut(s) 176, 363, 435, 624, 822, 2977, 3744
BseJI GATNNNNATC 1 cut(s) 1451
BseMI GCAATG 4 cut(s) 1626, 1734, 2614, 3283
BseMII CTCAG 9 cut(s) 439, 1086, 1091, 1267, 2060, 2163, 2371, 3590, 3672
BseNI ACTGG 9 cut(s) 975, 1105, 1880, 1945, 1952, 2552, 2623, 2748, 3179
BseRI GAGGAG 5 cut(s) 222, 398, 2059, 3533, 3593
BseSI GKGCMC 1 cut(s) 3665
BseX3I CGGCCG 2 cut(s) 595, 657
BseXI GCAGC 9 cut(s) 685, 1342, 1636, 1726, 2591, 2625, 2665, 2692, 3641
BseYI CCCAGC 1 cut(s) 2242
BsgI GTGCAG 1 cut(s) 3579
Bsh1285I CGRYCG 3 cut(s) 234, 598, 660
BshFI GGCC 9 cut(s) 351, 597, 659, 2089, 2551, 2561, 2631, 3324, 3688
BshNI GGYRCC 1 cut(s) 831
BshVI ATCGAT 1 cut(s) 2077
BsiEI CGRYCG 3 cut(s) 234, 598, 660
BsiHKAI GWGCWC 3 cut(s) 1053, 3480, 3665
BsiHKCI CYCGRG 5 cut(s) 439, 641, 728, 827, 2227
BslFI GGGAC 5 cut(s) 345, 2244, 2413, 2441, 3275
BsmAI GTCTC 8 cut(s) 470, 572, 686, 1614, 1986, 2791, 3539, 3595
BsmBI CGTCTC 1 cut(s) 3539
BsmFI GGGAC 5 cut(s) 345, 2244, 2413, 2441, 3275
BsmI GAATGC 2 cut(s) 1590, 3632
BsnI GGCC 9 cut(s) 351, 597, 659, 2089, 2551, 2561, 2631, 3324, 3688
Bso31I GGTCTC 3 cut(s) 572, 1614, 3595
BsoBI CYCGRG 5 cut(s) 439, 641, 728, 827, 2227
Bsp1286I GDGCHC 3 cut(s) 1053, 3480, 3665
Bsp13I TCCGGA 1 cut(s) 2903
Bsp1407I TGTACA 1 cut(s) 2964
Bsp1720I GCTNAGC 3 cut(s) 1095, 1100, 2790
BspACI CCGC 6 cut(s) 219, 285, 551, 1568, 2794, 3486
BspANI GGCC 9 cut(s) 351, 597, 659, 2089, 2551, 2561, 2631, 3324, 3688
BspCNI CTCAG 9 cut(s) 438, 1087, 1092, 1266, 2059, 2164, 2370, 3591, 3673
BspDI ATCGAT 1 cut(s) 2077
BspEI TCCGGA 1 cut(s) 2903
BspHI TCATGA 1 cut(s) 474
BspMAI CTGCAG 2 cut(s) 3199, 3660
BspMI ACCTGC 1 cut(s) 330
BspPI GGATC 8 cut(s) 1162, 1441, 2377, 2895, 2899, 2908, 3381, 3394
BspQI GCTCTTC 1 cut(s) 3158
BspT107I GGYRCC 1 cut(s) 831
BspTNI GGTCTC 3 cut(s) 572, 1614, 3595
BsrDI GCAATG 4 cut(s) 1626, 1734, 2614, 3283
BsrFI RCCGGY 1 cut(s) 597
BsrGI TGTACA 1 cut(s) 2964
BsrI ACTGG 9 cut(s) 975, 1105, 1880, 1945, 1952, 2552, 2623, 2748, 3179
BssAI RCCGGY 1 cut(s) 597
BssECI CCNNGG 8 cut(s) 393, 728, 729, 789, 879, 2185, 2556, 3615
BssNI GRCGYC 1 cut(s) 557
BssSI CACGAG 1 cut(s) 460
BssT1I CCWWGG 2 cut(s) 393, 879
Bst2BI CACGAG 1 cut(s) 460
Bst2UI CCWGG 2 cut(s) 1559, 2186
Bst6I CTCTTC 4 cut(s) 346, 394, 1617, 3158
BstACI GRCGYC 1 cut(s) 557
BstAPI GCANNNNNTGC 1 cut(s) 1633
BstAUI TGTACA 1 cut(s) 2964
BstC8I GCNNGC 7 cut(s) 455, 599, 2534, 2553, 3079, 3504, 3702
BstEII GGTNACC 1 cut(s) 524
BstENI CCTNNNNNAGG 2 cut(s) 1242, 2049
BstF5I GGATG 7 cut(s) 176, 363, 435, 624, 822, 2977, 3744
BstH2I RGCGCY 2 cut(s) 2423, 2738
BstHHI GCGC 2 cut(s) 2422, 2737
BstMAI GTCTC 8 cut(s) 470, 572, 686, 1614, 1986, 2791, 3539, 3595
BstMCI CGRYCG 3 cut(s) 234, 598, 660
BstNI CCWGG 2 cut(s) 1559, 2186
BstNSI RCATGY 4 cut(s) 219, 2350, 3506, 3725
BstPI GGTNACC 1 cut(s) 524
BstSFI CTRYAG 4 cut(s) 2914, 3195, 3451, 3656
BstSLI GKGCMC 1 cut(s) 3665
BstV1I GCAGC 9 cut(s) 685, 1342, 1636, 1726, 2591, 2625, 2665, 2692, 3641
BstX2I RGATCY 4 cut(s) 1446, 2382, 2900, 3386
BstXI CCANNNNNNTGG 2 cut(s) 880, 1367
BstYI RGATCY 4 cut(s) 1446, 2382, 2900, 3386
BstZI CGGCCG 2 cut(s) 595, 657
Bsu15I ATCGAT 1 cut(s) 2077
BsuI GTATCC 1 cut(s) 3126
BsuRI GGCC 9 cut(s) 351, 597, 659, 2089, 2551, 2561, 2631, 3324, 3688
BsuTUI ATCGAT 1 cut(s) 2077
BtgZI GCGATG 1 cut(s) 2489
BtsCI GGATG 7 cut(s) 176, 363, 435, 624, 822, 2977, 3744
BtsI GCAGTG 3 cut(s) 2955, 3653, 3665
BveI ACCTGC 1 cut(s) 330
Cac8I GCNNGC 7 cut(s) 455, 599, 2534, 2553, 3079, 3504, 3702
CciI TCATGA 1 cut(s) 474
CfoI GCGC 2 cut(s) 2422, 2737
Cfr10I RCCGGY 1 cut(s) 597
Cfr9I CCCGGG 1 cut(s) 728
ClaI ATCGAT 1 cut(s) 2077
CseI GACGC 4 cut(s) 546, 3022, 3508, 3556
Csp6I GTAC 7 cut(s) 198, 319, 832, 2767, 2965, 3103, 3641
CspCI CAANNNNNGTGG 2 cut(s) 1420, 1455
CviQI GTAC 7 cut(s) 198, 319, 832, 2767, 2965, 3103, 3641
DraIII CACNNNGTG 1 cut(s) 1057
DrdI GACNNNNNNGTC 1 cut(s) 220
DriI GACNNNNNGTC 1 cut(s) 2980
DseDI GACNNNNNNGTC 1 cut(s) 220
EaeI YGGCCR 3 cut(s) 595, 657, 2549
EagI CGGCCG 2 cut(s) 595, 657
Eam1104I CTCTTC 4 cut(s) 346, 394, 1617, 3158
Eam1105I GACNNNNNGTC 1 cut(s) 2980
EarI CTCTTC 4 cut(s) 346, 394, 1617, 3158
EciI GGCGGA 1 cut(s) 1583
Ecl136II GAGCTC 1 cut(s) 3478
EclXI CGGCCG 2 cut(s) 595, 657
Eco130I CCWWGG 2 cut(s) 393, 879
Eco147I AGGCCT 2 cut(s) 2561, 3688
Eco24I GRGCYC 1 cut(s) 3480
Eco31I GGTCTC 3 cut(s) 572, 1614, 3595
Eco32I GATATC 1 cut(s) 1062
Eco47III AGCGCT 1 cut(s) 2736
Eco52I CGGCCG 2 cut(s) 595, 657
Eco53kI GAGCTC 1 cut(s) 3478
Eco57I CTGAAG 4 cut(s) 884, 1139, 1247, 2324
Eco88I CYCGRG 5 cut(s) 439, 641, 728, 827, 2227
Eco91I GGTNACC 1 cut(s) 524
EcoICRI GAGCTC 1 cut(s) 3478
EcoNI CCTNNNNNAGG 2 cut(s) 1242, 2049
EcoO109I RGGNCCY 3 cut(s) 142, 359, 1298
EcoO65I GGTNACC 1 cut(s) 524
EcoRI GAATTC 2 cut(s) 1756, 1930
EcoRII CCWGG 2 cut(s) 1557, 2184
EcoRV GATATC 1 cut(s) 1062
EcoT14I CCWWGG 2 cut(s) 393, 879
EcoT22I ATGCAT 2 cut(s) 3418, 3508
EcoT38I GRGCYC 1 cut(s) 3480
ErhI CCWWGG 2 cut(s) 393, 879
Esp3I CGTCTC 1 cut(s) 3539
FalI AAGNNNNNCTT 2 cut(s) 1647, 1679
FaqI GGGAC 5 cut(s) 345, 2244, 2413, 2441, 3275
FauI CCCGC 2 cut(s) 212, 278
FauNDI CATATG 1 cut(s) 1683
FbaI TGATCA 2 cut(s) 540, 3709
FblI GTMKAC 3 cut(s) 2913, 2930, 3450
FokI GGATG 7 cut(s) 183, 350, 422, 611, 829, 2984, 3751
FriOI GRGCYC 1 cut(s) 3480
GlaI GCGC 2 cut(s) 2421, 2736
GsaI CCCAGC 1 cut(s) 2246
GsuI CTGGAG 2 cut(s) 632, 740
HaeII RGCGCY 2 cut(s) 2423, 2738
HaeIII GGCC 9 cut(s) 351, 597, 659, 2089, 2551, 2561, 2631, 3324, 3688
HgaI GACGC 4 cut(s) 546, 3022, 3508, 3556
HhaI GCGC 2 cut(s) 2422, 2737
Hin1I GRCGYC 1 cut(s) 557
Hin6I GCGC 2 cut(s) 2420, 2735
HinP1I GCGC 2 cut(s) 2420, 2735
HincII GTYRAC 7 cut(s) 775, 806, 824, 968, 1191, 1547, 3151
HindII GTYRAC 7 cut(s) 775, 806, 824, 968, 1191, 1547, 3151
HindIII AAGCTT 2 cut(s) 2492, 2992
HpaI GTTAAC 1 cut(s) 1191
Hpy99I CGWCG 1 cut(s) 3502
HpyAV CCTTC 4 cut(s) 1649, 2015, 2513, 3699
Hsp92I GRCGYC 1 cut(s) 557
HspAI GCGC 2 cut(s) 2420, 2735
KflI GGGWCCC 1 cut(s) 359
Kpn2I TCCGGA 1 cut(s) 2903
KpnI GGTACC 1 cut(s) 835
KroI GCCGGC 1 cut(s) 597
KroNI GCCGGC 1 cut(s) 599
Ksp22I TGATCA 2 cut(s) 540, 3709
KspAI GTTAAC 1 cut(s) 1191
LguI GCTCTTC 1 cut(s) 3158
LmnI GCTCC 9 cut(s) 330, 411, 1034, 1048, 1209, 2702, 3074, 3086, 3475
Lsp1109I GCAGC 9 cut(s) 685, 1342, 1636, 1726, 2591, 2625, 2665, 2692, 3641
LweI GCATC 3 cut(s) 2025, 2399, 3515
MfeI CAATTG 4 cut(s) 887, 2110, 2466, 2682
MflI RGATCY 4 cut(s) 1446, 2382, 2900, 3386
MhlI GDGCHC 3 cut(s) 1053, 3480, 3665
MlsI TGGCCA 1 cut(s) 2551
MluNI TGGCCA 1 cut(s) 2551
MmeI TCCRAC 6 cut(s) 235, 294, 629, 1014, 1161, 3381
Mox20I TGGCCA 1 cut(s) 2551
Mph1103I ATGCAT 2 cut(s) 3418, 3508
MroI TCCGGA 1 cut(s) 2903
MroNI GCCGGC 1 cut(s) 597
MroXI GAANNNNTTC 3 cut(s) 673, 1433, 1760
MscI TGGCCA 1 cut(s) 2551
MslI CAYNNNNRTG 4 cut(s) 2024, 2583, 3237, 3658
Msp20I TGGCCA 1 cut(s) 2551
MspA1I CMGCKG 3 cut(s) 610, 1094, 3486
MunI CAATTG 4 cut(s) 887, 2110, 2466, 2682
Mva1269I GAATGC 2 cut(s) 1590, 3632
MvaI CCWGG 2 cut(s) 1559, 2186
NaeI GCCGGC 1 cut(s) 599
NciI CCSGG 8 cut(s) 470, 502, 729, 730, 790, 2387, 3616, 3677
NdeI CATATG 1 cut(s) 1683
NgoMIV GCCGGC 1 cut(s) 597
NmeAIII GCCGAG 5 cut(s) 261, 429, 573, 685, 2581
NmuCI GTSAC 5 cut(s) 211, 236, 276, 485, 1777
NsiI ATGCAT 2 cut(s) 3418, 3508
NspI RCATGY 4 cut(s) 219, 2350, 3506, 3725
OliI CACNNNNGTG 1 cut(s) 3658
PaeI GCATGC 1 cut(s) 3506
PaeR7I CTCGAG 1 cut(s) 641
PagI TCATGA 1 cut(s) 474
PceI AGGCCT 2 cut(s) 2561, 3688
PciI ACATGT 1 cut(s) 3721
PciSI GCTCTTC 1 cut(s) 3158
PcsI WCGNNNNNNNCGW 1 cut(s) 3428
PctI GAATGC 2 cut(s) 1590, 3632
PdiI GCCGGC 1 cut(s) 599
PdmI GAANNNNTTC 3 cut(s) 673, 1433, 1760
PfeI GAWTC 9 cut(s) 480, 652, 724, 939, 1067, 1804, 2079, 2855, 3365
PflFI GACNNNGTC 2 cut(s) 230, 242
PflMI CCANNNNNTGG 1 cut(s) 2249
PfoI TCCNGGA 1 cut(s) 1557
PpuMI RGGWCCY 3 cut(s) 142, 359, 1298
PscI ACATGT 1 cut(s) 3721
Psp124BI GAGCTC 1 cut(s) 3480
Psp5II RGGWCCY 3 cut(s) 142, 359, 1298
Psp6I CCWGG 2 cut(s) 1557, 2184
PspEI GGTNACC 1 cut(s) 524
PspFI CCCAGC 1 cut(s) 2242
PspGI CCWGG 2 cut(s) 1557, 2184
PspPPI RGGWCCY 3 cut(s) 142, 359, 1298
PspXI VCTCGAGB 1 cut(s) 641
PsrI GAACNNNNNNTAC 2 cut(s) 2750, 2782
PstI CTGCAG 2 cut(s) 3199, 3660
PsuI RGATCY 4 cut(s) 1446, 2382, 2900, 3386
PsyI GACNNNGTC 2 cut(s) 230, 242
PvuII CAGCTG 2 cut(s) 610, 1094
RsaI GTAC 7 cut(s) 199, 320, 833, 2768, 2966, 3104, 3642
RsaNI GTAC 7 cut(s) 198, 319, 832, 2767, 2965, 3103, 3641
RseI CAYNNNNRTG 4 cut(s) 2024, 2583, 3237, 3658
SacI GAGCTC 1 cut(s) 3480
SapI GCTCTTC 1 cut(s) 3158
ScaI AGTACT 1 cut(s) 3642
SduI GDGCHC 3 cut(s) 1053, 3480, 3665
SfaNI GCATC 3 cut(s) 2025, 2399, 3515
SfcI CTRYAG 4 cut(s) 2914, 3195, 3451, 3656
Sfr274I CTCGAG 1 cut(s) 641
SlaI CTCGAG 1 cut(s) 641
SmaI CCCGGG 1 cut(s) 730
SmiMI CAYNNNNRTG 4 cut(s) 2024, 2583, 3237, 3658
SmlI CTYRAG 7 cut(s) 641, 857, 1382, 1835, 2414, 3210, 3425
SmoI CTYRAG 7 cut(s) 641, 857, 1382, 1835, 2414, 3210, 3425
SpeI ACTAGT 1 cut(s) 1040
SphI GCATGC 1 cut(s) 3506
SseBI AGGCCT 2 cut(s) 2561, 3688
SsiI CCGC 6 cut(s) 219, 285, 551, 1568, 2794, 3486
SspI AATATT 1 cut(s) 2697
SstI GAGCTC 1 cut(s) 3480
StuI AGGCCT 2 cut(s) 2561, 3688
StyI CCWWGG 2 cut(s) 393, 879
TaqII GACCGA 3 cut(s) 220, 232, 593
TatI WGTACW 5 cut(s) 197, 2766, 2964, 3102, 3640
TfiI GAWTC 9 cut(s) 480, 652, 724, 939, 1067, 1804, 2079, 2855, 3365
TseFI GTSAC 5 cut(s) 211, 236, 276, 485, 1777
Tsp45I GTSAC 5 cut(s) 211, 236, 276, 485, 1777
TspDTI ATGAA 7 cut(s) 1478, 1621, 1670, 1749, 1953, 2652, 3681
TspGWI ACGGA 1 cut(s) 3129
TspMI CCCGGG 1 cut(s) 728
Tth111I GACNNNGTC 2 cut(s) 230, 242
Van91I CCANNNNNTGG 1 cut(s) 2249
VneI GTGCAC 1 cut(s) 3661
XagI CCTNNNNNAGG 2 cut(s) 1242, 2049
XceI RCATGY 4 cut(s) 219, 2350, 3506, 3725
XcmI CCANNNNNNNNNTGG 1 cut(s) 2835
XhoI CTCGAG 1 cut(s) 641
XmaI CCCGGG 1 cut(s) 728
XmiI GTMKAC 3 cut(s) 2913, 2930, 3450
XmnI GAANNNNTTC 3 cut(s) 673, 1433, 1760
ZrmI AGTACT 1 cut(s) 3642
Zsp2I ATGCAT 2 cut(s) 3418, 3508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.