FvH4_3g13270
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014415
Physical Location & Seq
Forward (+)
7925597 .. 7930911
5315 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g13270.t3

Sequence Viewer

Length: 3786 bp
ATGGCTTCAACAACAACAACAACAACAAAGATGTCATCTCTACCGCTTCTTCTCATTCTCCTTGTCATTTCTTCTATTCTTGTTGAGGCAGAGCTCGACAGCAACACGACTCTAAAAGTTCTTTTGAAGGTGAAGAAGTCGTTTTTGGAAGACCCAACATCCGAAACAGCCTTGCATGGATGGTCTGAAAGCAACCCGAATTTCTGTACTTGGAGAGGAGTGACATGCGGGTCTGACTCGGTCGGTGACTCGCTCCAAGTGACCGGACTCAACCTCTCCGACTCGTCACTCGGAGGGTCGATATCTCCCCAACTCGGTAGTTTGAAGTACCTGCTCCACCTCGACCTCTCTTCTAACGGCCTCAAGGGTCCCATTCCACCAGCCCTCTCAAACCTCTCTTCCTTGGAATCTTTGCTCCTCTTCTCCAACCAACTCGGTGGCCCCATCCCGAGTGAACTCGGCTCGCTGACGAGTCTCCGAGTCATGAGAATCGGTGACAATGAGCTAACCGGGTCCATTCCGGCCTCATTTGGTAACCTTGTGAATTTGGTCACTCTTGGGCTCGCGTCATGTGGTCTCAGCGGTCCAATACCGCCCCAACTCGGCCGGCTCGATCAGCTGGAGAATTTAATCCTCCAGCTGAACCAACTCGAGGGTCCAATCCCGGCCGAGTTGGGAAACTGCACTAGCCTCACTGTGTTCACAGCTGCTCAAAATAAACTCAACAGCTCGATTCCCCGGGAATTGGGCCAGCTCACCAATCTGCAGCTACTCAATTTGGTCAACAACAGCCTCTCCGGCGAGATACCGAGTCAACTCGGTGGGATGAGTCAACTCGAGTACCTGAATTTGATGGGGAACCAACTGGAGGGTGCAATACCAAAGTCCTTGGCTCAATTGGGCAATCTTCAGAATCTTGATTTGTCTGTTAACAAGCTCACAGGAGGAATCCCAGAGGAACTAGGTAACATGAGTCAGCTGGTTTACTTGGTTTTGTCCAACAACAATCTCTCTGGTGTTGTACCAAAGACTATATGCTCCAATGCAACAAGTTTGGAGCACTTGATGATATCTGATGCTCAAATTTTCGGCGAAATCCCTGCTGGGCTGAGCCAGTGTCAATCCCTGAAGCAACTTGACTTGTCCAACAATTCAATGAATGGATCAATTCCACTTGAGCTTTATAGCTTGGTGGAGTTAACTGACCTCTTGCTCCACAACAACAGCTTGGTTGGTTCAATTTCTCCTTATATAGGCAACCTCAGCAATCTTCTGAGTCTTGTTTTGTATCACAACAATTTGCAGGGTCCTCTGCCGAAAGAAATTGGTATGCTTGGGCAGCTTGAGATTTTGTATCTCTACGATAACCAGCTCAGTGGTGAAATACCAATTGAGATTGGAAATTGTTCGAGTTTGCAGATGATTGATTTCTATGGAAACAAGTTCAGTGGGGAGATTCCGATCACTATTGGGAGGCTCAAGGATTTGAACTTTCTTCATATCAGGCAGAATGAGCTAGTGGGTGAGCTTCCTGCCACATTGGGAAACTGTCATCAACTAACTATCCTGGACTTGGCGGATAACCATTTGTCTGGTGGCATTCCTATTACATTTGGGTCTCTTCAAGCAATGGAGCAGTTCATGCTGTACAACAATTCACTTGAAGGTAATCTTCCTGAAACATTGGTTAATATGGTGAATTTGACTAGAGTGAATCTGTCAAAAAACAAATTGAATGGAAGCATTGCTGCATTGTGTAGCTCGAATTCATTCCTTTCTTTTGATGTCACTGACAATGCATTTGATGGTGAGATTCCTCCCCATCTGGGTAATTCAACCTCCCTTGAGAGGTTGAGATTGGGGAACAACCAGTTTACGGGTCCAATCCCGGTGACATTGGGAAAAATCAGTGAGCTATCGCTGCTAGATGTGTCAGGGAATTCACTCACCGGATCCGTGCCAGCTGAGTTTTCATCCTGCAAGAAACTGTCCCATTTTGATTTGAACAACAACCTTCTGTCTGGCACTATCCCAGTATGGCTTGGAAGTTTGCCTCAGCTAGGAGAATTGAAGCTTTCCTCGAATCACTTCACAGGGACACTTCCCCAAGAGTTGTTCAATTGTTCTAAACTGCTAGTGCTTTCTTTGAATGACAATTTGCTCAATGGAACCCTCCCTGCTGAGATTGGTAACTTGGATTCTCTTAATGTCTTAAACCTCAACCACAACCAATTCTCGGGACCAATCCCTCCAGCCATTGGGAAGCTAGGCAATCTCTATGAGCTTAAGCTCTCACAGAACAGCTTTGATGGTGACATACCCTATGAGGTCGGACAACTCCAAAATCTTCAGAGCATGTTAGACCTCAGTTACAATAAACTGAGTGGTCAGATCCCGGCCTCTATTGGGACACTCTCAAAACTTGAGGCACTTGATCTATCTCACAATCAACTTAATGGAGAAGTCCCCTCTACTATTGGTGCGATGAGTAGCTTGGGAAAGCTTAATCTCACTTACAACAATCTGGAAGGAAAACTAAGCAAGCAACTCTCACACTGGCCAGCCGAGGCCTTTGTAGGGAATTTACATCTTTGTGGGAACCCTCTTGGTAGCTGCAATGGCAGCAAGAACCAGTCAGGTCCAAATGAAGCGACTGTGGTCGTCATCTCTGCATTATGCACATTAGCTGCAATTGCTCTGCTAATATTTGGAGCTGCTTCTTTCTTGAAACACAAGCGAGAAGCTTTTAGAAAATCCAGTGAAGTGAACTATGTGTACTCATCGAGTTCTTCTCATACTAAGCGGAGACTGCTATTTTCAAATGGTTCTGTCAAGCCAGATTTCAAGTGGAAAGATATCATGGACGCCACCAACAATCTAAGTAAAGAGTTTGTGATTGGATCAGGAGGATCAGGAGTAGTGTACAGAGCTGAATTGGCTACTGGAGAAATTGTGGCAGTGAAGAAGATTTTGTACAAGGATGACCTTATGTCAAATAAAAGCTTCACAAGGGAGATTAAGACGCTTGGGAGGATAAGGCACAGGCATTTAGTGAAGCTAATGGGGTACTGTAGCAACAAAGGAGCAGGCTCCAATCTGCTGATCTATGAGTACATGGAAAACGGAAGCGTATGGGATTGGATACACCAAGAACAAGTTAACAGCAAGAAGAAGAGCCTTGATTGGGAAGCAAGGTTAAAGATTGCAGTTGGGCTAGCTCAAGGAGTGGAGTATCTCCACCATGACTGTGCGCCAAAGATCATTCACAGGGACATCAAATCCAGCAATGTCTTGCTAGACTCGAATATGGAGGCACATCTTGGGGATTTTGGCCTTGCCAAGACACTCGATGACAACTTTGAGTCTAACACAGAATCAAATACATGGTTTGCTGGATCCTATGGTTACATAGCACCAGAGTATGCATATTCGCTCAAGGCCACAGAAAAGAGTGATGTCTACAGCATGGGAATTGTGTTAATGGAGATTGTCAGCGGAAAAATGCCGACAGACACGTCTTTCGGAGTGGAGATGGACATGGTGAGATGGGTGGAGACGCATATTGAAATGCAAGGTCATGCTCGTGAGGAATTGATAGACCCTGCACTGAGACCACTCTTATCCGGGGAAGAATCTGCAGCGTTCCAAGTGCTGGAGATAGCACTGCAGTGCACAAAGACCAGCCCGGCAGAGAGGCCTTCATCACGGGAAGCTTGTGATCAGCTACTACATGTTTTCAATCATAGGATGGCGGAGTTTGATAAGACGAACATAGATAAGGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1262

Amino Acids

136.53

Weight (kDa)

5.26

Isoelectric Point (pI)

34.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 39 - 76 8e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 152 - 261 2e-09 Leucine-rich repeat region
LRR_8 PF13855 253 - 313 1.5e-06 Leucine rich repeat
LRR_14 PF23598 271 - 358 2.5e-07 Leucine-rich repeat region
LRR_14 PF23598 369 - 477 1.5e-06 Leucine-rich repeat region
LRR_14 PF23598 484 - 577 2.1e-06 Leucine-rich repeat region
LRR_8 PF13855 519 - 578 3.1e-06 Leucine rich repeat
LRR_8 PF13855 638 - 697 2.7e-07 Leucine rich repeat
LRR_8 PF13855 685 - 745 7.4e-06 Leucine rich repeat
LRR_14 PF23598 709 - 785 2.1e-06 Leucine-rich repeat region
LRR_8 PF13855 758 - 818 9.4e-07 Leucine rich repeat
LRR_14 PF23598 763 - 840 6.5e-07 Leucine-rich repeat region
LRR_8 PF13855 786 - 842 2.5e-06 Leucine rich repeat
Pkinase PF00069 965 - 1241 2.5e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 965 - 1240 6.8e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014415)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20140
fragaria_vesca FvH4_3g13270 FvH4_3g13270 FvH4_3g13270
malus_domestica MD05G1243600.v1.1 MD10G1225500.v1.1
prunus_persica Prupe.4G120100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0021741
rosa_laevigata RLG00000032624
rosa_multiflora Rmu_ssc0000233.1_g000008
rosa_roxburghii Rroxscaffold_1G00056980
rosa_rugosa Rorug05G0068900
rosa_samantha Rh5AG157400 Rh5BG157100 Rh5CG171200 Rh5DG157300
rosa_wichuraiana Rw5G014030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 229, 3514
Acc36I ACCTGC 1 cut(s) 339
AccB7I CCANNNNNTGG 2 cut(s) 2258, 3652
AccI GTMKAC 1 cut(s) 3459
AccII CGCG 1 cut(s) 566
AciI CCGC 8 cut(s) 44, 228, 582, 593, 1577, 2803, 3495, 3752
AclWI GGATC 8 cut(s) 1171, 1947, 1960, 2386, 2908, 2917, 3390, 3403
AcoI YGGCCR 3 cut(s) 604, 666, 2558
AcsI RAATTY 9 cut(s) 199, 544, 625, 847, 1083, 1699, 1765, 1939, 2581
AcuI CTGAAG 3 cut(s) 893, 1148, 2333
AcyI GRCGYC 1 cut(s) 2865
AflII CTTAAG 1 cut(s) 2285
AflIII ACRYGT 2 cut(s) 3513, 3730
AhdI GACNNNNNGTC 1 cut(s) 2989
AjiI CACGTC 1 cut(s) 3516
AjnI CCWGG 1 cut(s) 1566
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
AleI CACNNNNGTG 1 cut(s) 3667
Alw21I GWGCWC 3 cut(s) 96, 1062, 3674
Alw26I GTCTC 6 cut(s) 479, 581, 1623, 2800, 3548, 3604
Alw44I GTGCAC 1 cut(s) 3670
AlwI GGATC 8 cut(s) 1171, 1947, 1960, 2386, 2908, 2917, 3390, 3403
Ama87I CYCGRG 5 cut(s) 448, 650, 738, 836, 2236
ApaLI GTGCAC 1 cut(s) 3670
ApoI RAATTY 9 cut(s) 199, 544, 625, 847, 1083, 1699, 1765, 1939, 2581
ArsI GACNNNNNNTTYG 4 cut(s) 1784, 1816, 3503, 3535
Asp700I GAANNNNTTC 3 cut(s) 1405, 1769, 2087
AspLEI GCGC 1 cut(s) 3253
AsuC2I CCSGG 8 cut(s) 511, 665, 739, 740, 1889, 2396, 3625, 3686
AsuNHI GCTAGC 1 cut(s) 3214
AvaI CYCGRG 5 cut(s) 448, 650, 738, 836, 2236
AvaII GGWCC 8 cut(s) 368, 513, 584, 656, 1307, 1880, 2240, 2639
BaeGI GKGCMC 1 cut(s) 3674
BaeI ACNNNNGTAYC 2 cut(s) 824, 857
BalI TGGCCA 1 cut(s) 2560
BamHI GGATCC 2 cut(s) 1952, 3395
BanII GRGCYC 2 cut(s) 96, 564
BarI GAAGNNNNNNTAC 2 cut(s) 2957, 2989
BauI CACGAG 1 cut(s) 3582
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 3 cut(s) 96, 1062, 3674
BbvCI CCTCAGC 2 cut(s) 1262, 2055
BccI CCATC 9 cut(s) 174, 452, 847, 1799, 1830, 2303, 3526, 3540, 3742
BceAI ACGGC 1 cut(s) 373
BcgI CGANNNNNNTGC 2 cut(s) 1082, 1116
BciT130I CCWGG 1 cut(s) 1568
BciVI GTATCC 1 cut(s) 3135
BclI TGATCA 1 cut(s) 3718
BcnI CCSGG 8 cut(s) 511, 665, 739, 740, 1889, 2396, 3625, 3686
BcoDI GTCTC 6 cut(s) 479, 581, 1623, 2800, 3548, 3604
BfmI CTRYAG 5 cut(s) 764, 3070, 3460, 3636, 3665
BfrI CTTAAG 1 cut(s) 2285
BfuAI ACCTGC 1 cut(s) 339
BfuI GTATCC 1 cut(s) 3135
BglI GCCNNNNNGGC 1 cut(s) 798
BlpI GCTNAGC 1 cut(s) 1109
Bme1390I CCNGG 9 cut(s) 511, 665, 739, 740, 1568, 1889, 2396, 3625, 3686
Bme18I GGWCC 8 cut(s) 368, 513, 584, 656, 1307, 1880, 2240, 2639
BmeRI GACNNNNNGTC 1 cut(s) 2989
BmeT110I CYCGRG 5 cut(s) 448, 650, 738, 836, 2236
BmgBI CACGTC 1 cut(s) 3516
BmrFI CCNGG 9 cut(s) 511, 665, 739, 740, 1568, 1889, 2396, 3625, 3686
BmrI ACTGGG 1 cut(s) 2027
BmsI GCATC 1 cut(s) 1066
BmtI GCTAGC 1 cut(s) 3218
BmuI ACTGGG 1 cut(s) 2027
BoxI GACNNNNGTC 1 cut(s) 2657
BpiI GAAGAC 1 cut(s) 156
BplI GAGNNNNNCTC 2 cut(s) 2776, 2808
BpmI CTGGAG 6 cut(s) 620, 641, 887, 2235, 2964, 3674
Bpu10I CCTNAGC 2 cut(s) 1262, 2055
Bpu1102I GCTNAGC 1 cut(s) 1109
BpuEI CTTGAG 8 cut(s) 347, 1196, 1364, 1463, 1865, 2444, 3204, 3419
BpuMI CCSGG 8 cut(s) 511, 665, 739, 740, 1889, 2396, 3625, 3686
BsaBI GATNNNNATC 1 cut(s) 1460
BsaHI GRCGYC 1 cut(s) 2865
BsaI GGTCTC 3 cut(s) 581, 1623, 3604
BsaJI CCNNGG 6 cut(s) 402, 737, 738, 888, 2565, 3624
BsaWI WCCGGW 2 cut(s) 263, 1949
BsaXI ACNNNNNCTCC 4 cut(s) 779, 809, 2937, 2967
Bse118I RCCGGY 1 cut(s) 606
Bse1I ACTGG 8 cut(s) 870, 1114, 1870, 2033, 2561, 2632, 2757, 2947
Bse3DI GCAATG 4 cut(s) 1635, 1743, 2623, 3292
Bse8I GATNNNNATC 1 cut(s) 1460
BseBI CCWGG 1 cut(s) 1568
BseDI CCNNGG 6 cut(s) 402, 737, 738, 888, 2565, 3624
BseGI GGATG 7 cut(s) 158, 185, 444, 831, 1973, 2986, 3753
BseJI GATNNNNATC 1 cut(s) 1460
BseMI GCAATG 4 cut(s) 1635, 1743, 2623, 3292
BseNI ACTGG 8 cut(s) 870, 1114, 1870, 2033, 2561, 2632, 2757, 2947
BseRI GAGGAG 2 cut(s) 231, 407
BseSI GKGCMC 1 cut(s) 3674
BseX3I CGGCCG 2 cut(s) 604, 666
BseYI CCCAGC 1 cut(s) 1103
BsgI GTGCAG 2 cut(s) 667, 3588
Bsh1236I CGCG 1 cut(s) 566
Bsh1285I CGRYCG 3 cut(s) 243, 607, 669
BsiEI CGRYCG 3 cut(s) 243, 607, 669
BsiHKAI GWGCWC 3 cut(s) 96, 1062, 3674
BsiHKCI CYCGRG 5 cut(s) 448, 650, 738, 836, 2236
BslFI GGGAC 7 cut(s) 354, 1975, 2110, 2253, 2422, 2450, 3284
BsmAI GTCTC 6 cut(s) 479, 581, 1623, 2800, 3548, 3604
BsmBI CGTCTC 1 cut(s) 3548
BsmFI GGGAC 7 cut(s) 354, 1975, 2110, 2253, 2422, 2450, 3284
BsmI GAATGC 1 cut(s) 1599
Bso31I GGTCTC 3 cut(s) 581, 1623, 3604
BsoBI CYCGRG 5 cut(s) 448, 650, 738, 836, 2236
Bsp1286I GDGCHC 4 cut(s) 96, 564, 1062, 3674
Bsp1407I TGTACA 3 cut(s) 1647, 2922, 2973
Bsp1720I GCTNAGC 1 cut(s) 1109
BspACI CCGC 8 cut(s) 44, 228, 582, 593, 1577, 2803, 3495, 3752
BspFNI CGCG 1 cut(s) 566
BspHI TCATGA 1 cut(s) 483
BspMAI CTGCAG 3 cut(s) 768, 3640, 3669
BspMI ACCTGC 1 cut(s) 339
BspOI GCTAGC 1 cut(s) 3218
BspPI GGATC 8 cut(s) 1171, 1947, 1960, 2386, 2908, 2917, 3390, 3403
BspQI GCTCTTC 1 cut(s) 3167
BspTI CTTAAG 1 cut(s) 2285
BspTNI GGTCTC 3 cut(s) 581, 1623, 3604
BsrDI GCAATG 4 cut(s) 1635, 1743, 2623, 3292
BsrFI RCCGGY 1 cut(s) 606
BsrGI TGTACA 3 cut(s) 1647, 2922, 2973
BsrI ACTGG 8 cut(s) 870, 1114, 1870, 2033, 2561, 2632, 2757, 2947
BssAI RCCGGY 1 cut(s) 606
BssECI CCNNGG 6 cut(s) 402, 737, 738, 888, 2565, 3624
BssNI GRCGYC 1 cut(s) 2865
BssSI CACGAG 1 cut(s) 3582
BssT1I CCWWGG 2 cut(s) 402, 888
Bst2BI CACGAG 1 cut(s) 3582
Bst2UI CCWGG 1 cut(s) 1568
Bst4CI ACNGT 6 cut(s) 697, 1550, 1989, 2656, 3071, 3248
Bst6I CTCTTC 5 cut(s) 355, 403, 425, 1626, 3167
BstACI GRCGYC 1 cut(s) 2865
BstAFI CTTAAG 1 cut(s) 2285
BstAPI GCANNNNNTGC 1 cut(s) 1642
BstAUI TGTACA 3 cut(s) 1647, 2922, 2973
BstC8I GCNNGC 9 cut(s) 464, 564, 608, 752, 1962, 2543, 2562, 3088, 3216
BstEII GGTNACC 1 cut(s) 533
BstENI CCTNNNNNAGG 2 cut(s) 1251, 2058
BstF5I GGATG 7 cut(s) 158, 185, 444, 831, 1973, 2986, 3753
BstFNI CGCG 1 cut(s) 566
BstHHI GCGC 1 cut(s) 3253
BstMAI GTCTC 6 cut(s) 479, 581, 1623, 2800, 3548, 3604
BstMCI CGRYCG 3 cut(s) 243, 607, 669
BstNI CCWGG 1 cut(s) 1568
BstNSI RCATGY 3 cut(s) 228, 2359, 3734
BstPAI GACNNNNGTC 1 cut(s) 2657
BstPI GGTNACC 1 cut(s) 533
BstSCI CCNGG 9 cut(s) 509, 663, 737, 738, 1566, 1887, 2394, 3623, 3684
BstSFI CTRYAG 5 cut(s) 764, 3070, 3460, 3636, 3665
BstSLI GKGCMC 1 cut(s) 3674
BstUI CGCG 1 cut(s) 566
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 3 cut(s) 1952, 2391, 3395
BstXI CCANNNNNNTGG 3 cut(s) 437, 1376, 1592
BstYI RGATCY 3 cut(s) 1952, 2391, 3395
BstZI CGGCCG 2 cut(s) 604, 666
BsuI GTATCC 1 cut(s) 3135
BtgZI GCGATG 1 cut(s) 2498
BtrI CACGTC 1 cut(s) 3516
BtsCI GGATG 7 cut(s) 158, 185, 444, 831, 1973, 2986, 3753
BtsI GCAGTG 3 cut(s) 2964, 3662, 3674
BveI ACCTGC 1 cut(s) 339
Cac8I GCNNGC 9 cut(s) 464, 564, 608, 752, 1962, 2543, 2562, 3088, 3216
CciI TCATGA 1 cut(s) 483
CfoI GCGC 1 cut(s) 3253
Cfr10I RCCGGY 1 cut(s) 606
Cfr9I CCCGGG 1 cut(s) 738
CseI GACGC 4 cut(s) 555, 2873, 3031, 3565
CspCI CAANNNNNGTGG 2 cut(s) 1429, 1464
DrdI GACNNNNNNGTC 2 cut(s) 229, 3514
DriI GACNNNNNGTC 1 cut(s) 2989
DseDI GACNNNNNNGTC 2 cut(s) 229, 3514
EaeI YGGCCR 3 cut(s) 604, 666, 2558
EagI CGGCCG 2 cut(s) 604, 666
Eam1104I CTCTTC 5 cut(s) 355, 403, 425, 1626, 3167
Eam1105I GACNNNNNGTC 1 cut(s) 2989
EarI CTCTTC 5 cut(s) 355, 403, 425, 1626, 3167
EciI GGCGGA 2 cut(s) 1592, 3767
Ecl136II GAGCTC 1 cut(s) 94
EclXI CGGCCG 2 cut(s) 604, 666
Eco130I CCWWGG 2 cut(s) 402, 888
Eco147I AGGCCT 2 cut(s) 2570, 3697
Eco24I GRGCYC 2 cut(s) 96, 564
Eco31I GGTCTC 3 cut(s) 581, 1623, 3604
Eco32I GATATC 3 cut(s) 303, 1071, 2857
Eco47I GGWCC 8 cut(s) 368, 513, 584, 656, 1307, 1880, 2240, 2639
Eco52I CGGCCG 2 cut(s) 604, 666
Eco53kI GAGCTC 1 cut(s) 94
Eco57I CTGAAG 3 cut(s) 893, 1148, 2333
Eco88I CYCGRG 5 cut(s) 448, 650, 738, 836, 2236
Eco91I GGTNACC 1 cut(s) 533
EcoICRI GAGCTC 1 cut(s) 94
EcoNI CCTNNNNNAGG 2 cut(s) 1251, 2058
EcoO109I RGGNCCY 2 cut(s) 368, 1307
EcoO65I GGTNACC 1 cut(s) 533
EcoRI GAATTC 2 cut(s) 1765, 1939
EcoRII CCWGG 1 cut(s) 1566
EcoRV GATATC 3 cut(s) 303, 1071, 2857
EcoT14I CCWWGG 2 cut(s) 402, 888
EcoT22I ATGCAT 2 cut(s) 1801, 3427
EcoT38I GRGCYC 2 cut(s) 96, 564
ErhI CCWWGG 2 cut(s) 402, 888
Esp3I CGTCTC 1 cut(s) 3548
FalI AAGNNNNNCTT 2 cut(s) 1656, 1688
FaqI GGGAC 7 cut(s) 354, 1975, 2110, 2253, 2422, 2450, 3284
FauI CCCGC 1 cut(s) 221
FbaI TGATCA 1 cut(s) 3718
FblI GTMKAC 1 cut(s) 3459
FokI GGATG 7 cut(s) 145, 192, 431, 838, 1960, 2993, 3760
FriOI GRGCYC 2 cut(s) 96, 564
GlaI GCGC 1 cut(s) 3252
GsaI CCCAGC 1 cut(s) 1107
GsuI CTGGAG 6 cut(s) 620, 641, 887, 2235, 2964, 3674
HgaI GACGC 4 cut(s) 555, 2873, 3031, 3565
HhaI GCGC 1 cut(s) 3253
Hin1I GRCGYC 1 cut(s) 2865
Hin6I GCGC 1 cut(s) 3251
HinP1I GCGC 1 cut(s) 3251
HincII GTYRAC 6 cut(s) 784, 815, 833, 931, 1200, 3160
HindII GTYRAC 6 cut(s) 784, 815, 833, 931, 1200, 3160
HindIII AAGCTT 5 cut(s) 2072, 2501, 2742, 3001, 3711
HpaI GTTAAC 3 cut(s) 931, 1200, 3160
HpyAV CCTTC 5 cut(s) 121, 1658, 2024, 2522, 3708
HpyCH4III ACNGT 6 cut(s) 697, 1550, 1989, 2656, 3071, 3248
HpyCH4IV ACGT 1 cut(s) 3515
HpySE526I ACGT 1 cut(s) 3515
Hsp92I GRCGYC 1 cut(s) 2865
HspAI GCGC 1 cut(s) 3251
KflI GGGWCCC 1 cut(s) 368
KroI GCCGGC 1 cut(s) 606
KroNI GCCGGC 1 cut(s) 608
Ksp22I TGATCA 1 cut(s) 3718
KspAI GTTAAC 3 cut(s) 931, 1200, 3160
LguI GCTCTTC 1 cut(s) 3167
LweI GCATC 1 cut(s) 1066
MaeII ACGT 1 cut(s) 3515
MfeI CAATTG 4 cut(s) 896, 1389, 2119, 2691
MflI RGATCY 3 cut(s) 1952, 2391, 3395
MhlI GDGCHC 4 cut(s) 96, 564, 1062, 3674
MlsI TGGCCA 1 cut(s) 2560
MluNI TGGCCA 1 cut(s) 2560
MmeI TCCRAC 5 cut(s) 303, 450, 1023, 1170, 2311
Mox20I TGGCCA 1 cut(s) 2560
Mph1103I ATGCAT 2 cut(s) 1801, 3427
MroNI GCCGGC 1 cut(s) 606
MroXI GAANNNNTTC 3 cut(s) 1405, 1769, 2087
MscI TGGCCA 1 cut(s) 2560
MslI CAYNNNNRTG 4 cut(s) 2592, 3246, 3582, 3667
Msp20I TGGCCA 1 cut(s) 2560
MspA1I CMGCKG 7 cut(s) 582, 619, 640, 707, 979, 1964, 3495
MspCI CTTAAG 1 cut(s) 2285
MspR9I CCNGG 9 cut(s) 511, 665, 739, 740, 1568, 1889, 2396, 3625, 3686
MunI CAATTG 4 cut(s) 896, 1389, 2119, 2691
Mva1269I GAATGC 1 cut(s) 1599
MvaI CCWGG 1 cut(s) 1568
MvnI CGCG 1 cut(s) 566
NaeI GCCGGC 1 cut(s) 608
NciI CCSGG 8 cut(s) 511, 665, 739, 740, 1889, 2396, 3625, 3686
NgoMIV GCCGGC 1 cut(s) 606
NheI GCTAGC 1 cut(s) 3214
NmeAIII GCCGAG 4 cut(s) 438, 582, 694, 2590
NmuCI GTSAC 9 cut(s) 220, 245, 259, 285, 494, 550, 1786, 1891, 2312
NsiI ATGCAT 2 cut(s) 1801, 3427
NspI RCATGY 3 cut(s) 228, 2359, 3734
OliI CACNNNNGTG 1 cut(s) 3667
PaeR7I CTCGAG 2 cut(s) 650, 836
PagI TCATGA 1 cut(s) 483
PceI AGGCCT 2 cut(s) 2570, 3697
PciI ACATGT 1 cut(s) 3730
PciSI GCTCTTC 1 cut(s) 3167
PcsI WCGNNNNNNNCGW 1 cut(s) 609
PctI GAATGC 1 cut(s) 1599
PdiI GCCGGC 1 cut(s) 608
PdmI GAANNNNTTC 3 cut(s) 1405, 1769, 2087
PflFI GACNNNGTC 1 cut(s) 239
PflMI CCANNNNNTGG 2 cut(s) 2258, 3652
PfoI TCCNGGA 1 cut(s) 1566
PpuMI RGGWCCY 2 cut(s) 368, 1307
PscI ACATGT 1 cut(s) 3730
PshAI GACNNNNGTC 1 cut(s) 2657
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 2 cut(s) 368, 1307
Psp6I CCWGG 1 cut(s) 1566
PspEI GGTNACC 1 cut(s) 533
PspFI CCCAGC 1 cut(s) 1103
PspGI CCWGG 1 cut(s) 1566
PspPPI RGGWCCY 2 cut(s) 368, 1307
PspXI VCTCGAGB 2 cut(s) 650, 836
PsrI GAACNNNNNNTAC 2 cut(s) 2759, 2791
PstI CTGCAG 3 cut(s) 768, 3640, 3669
PsuI RGATCY 3 cut(s) 1952, 2391, 3395
PsyI GACNNNGTC 1 cut(s) 239
PvuII CAGCTG 5 cut(s) 619, 640, 707, 979, 1964
RseI CAYNNNNRTG 4 cut(s) 2592, 3246, 3582, 3667
SacI GAGCTC 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 3167
ScrFI CCNGG 9 cut(s) 511, 665, 739, 740, 1568, 1889, 2396, 3625, 3686
SduI GDGCHC 4 cut(s) 96, 564, 1062, 3674
SfaNI GCATC 1 cut(s) 1066
SfcI CTRYAG 5 cut(s) 764, 3070, 3460, 3636, 3665
Sfr274I CTCGAG 2 cut(s) 650, 836
SinI GGWCC 8 cut(s) 368, 513, 584, 656, 1307, 1880, 2240, 2639
SlaI CTCGAG 2 cut(s) 650, 836
SmaI CCCGGG 1 cut(s) 740
SmiMI CAYNNNNRTG 4 cut(s) 2592, 3246, 3582, 3667
SseBI AGGCCT 2 cut(s) 2570, 3697
SsiI CCGC 8 cut(s) 44, 228, 582, 593, 1577, 2803, 3495, 3752
SspI AATATT 1 cut(s) 2706
SstI GAGCTC 1 cut(s) 96
StuI AGGCCT 2 cut(s) 2570, 3697
StyD4I CCNGG 9 cut(s) 509, 663, 737, 738, 1566, 1887, 2394, 3623, 3684
StyI CCWWGG 2 cut(s) 402, 888
TaaI ACNGT 6 cut(s) 697, 1550, 1989, 2656, 3071, 3248
TaiI ACGT 1 cut(s) 3518
TaqII GACCGA 1 cut(s) 229
TatI WGTACW 6 cut(s) 206, 1647, 2775, 2922, 2973, 3111
TseFI GTSAC 9 cut(s) 220, 245, 259, 285, 494, 550, 1786, 1891, 2312
Tsp45I GTSAC 9 cut(s) 220, 245, 259, 285, 494, 550, 1786, 1891, 2312
TspDTI ATGAA 7 cut(s) 1172, 1487, 1630, 1758, 1962, 2661, 3690
TspGWI ACGGA 2 cut(s) 1945, 3138
TspMI CCCGGG 1 cut(s) 738
Tth111I GACNNNGTC 1 cut(s) 239
Van91I CCANNNNNTGG 2 cut(s) 2258, 3652
Vha464I CTTAAG 1 cut(s) 2285
VneI GTGCAC 1 cut(s) 3670
VpaK11BI GGWCC 8 cut(s) 368, 513, 584, 656, 1307, 1880, 2240, 2639
XagI CCTNNNNNAGG 2 cut(s) 1251, 2058
XapI RAATTY 9 cut(s) 199, 544, 625, 847, 1083, 1699, 1765, 1939, 2581
XceI RCATGY 3 cut(s) 228, 2359, 3734
XcmI CCANNNNNNNNNTGG 2 cut(s) 1592, 2844
XhoI CTCGAG 2 cut(s) 650, 836
XmaI CCCGGG 1 cut(s) 738
XmiI GTMKAC 1 cut(s) 3459
XmnI GAANNNNTTC 3 cut(s) 1405, 1769, 2087
Zsp2I ATGCAT 2 cut(s) 1801, 3427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.