MD05G1243600.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0014415
Physical Location & Seq
Reverse (-)
37615347 .. 37619542
4196 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1243600.v1.1.491

Sequence Viewer

Length: 3774 bp
ATGGCTTTAGTAACAGATGCAATTTTGCCGCTTTTTCTTGCTCTGTTTCTGCATCTTTCATTAGGGCTTATGTCCTGTGAGGGAAAGCTCGACGGAAATACAACTTTGAGCGTTCTTTTGGAGGTGAAGAGGGCAATTGAGGAAGACCCAAAAAATGTTTTGCATGGATGGTCCGAAAGCGACCAAAATTTTTGTACTTGGAGGGGTGTGACTTGTGGGTTTGACTCGGTGGACAACTCGGTCCAAGTGGTGGGGCTCAACCTTTCCGACTCGTCACTCGCCGGGTTGATATCTCCCTCACTCGGTAGTTTGCAAAACCTGCTCCACCTTGATCTTTCTTCTAACGGCCTCTCGGGTCCCATCCCGCCAACCCTCTCTAACCTCACGTCCTTGGAATCTTTACTTCTCTTATCGAACCAGCTCAGTGGACCCATTCCGTCTCAGCTCGGCTCGCTGACGAGTCTCCGAGTCATGAGAATCGGCGACAATGGGCTCACTGGGTCCATTCCCGCCACTTTGGGCAATCTTGTCAATTTGGTCACGCTTGGTTTGGCCTCATGCAGCCTCAACGGTACAATACCTCCCCAACTCGGCCGACTCGGCCTACTGGAGAACTTAATCCTGCAGCTCAATCAGCTCGAGGGTCCGATTCCGGCCGAGTTGGGGAACTGTTCCAGCCTCACTGTATTCACAGCTGCTAACAATACACTCAACGGAACAATCCCCGCGGAGCTCGGCCGTCTCAGCAACCTCGAGCTCCTGAATTTCGGCAACAACAGCCTCACTGGCCAAATCCCAAGTCAACTTGGTGAACTGAGTCAACTCGAATACATGAATTTCATGGGGAACCAACTTGAAGGTCCAGTCCCAAAGTCCATATCTCAACTGGGCAATCTTCAGAGTCTTGATTTATCCATGAACAAGCTCACAGGAGGAGTTCCCGACGTATTCGGCACCATGTCCCAGCTGGCAACCTTAGTTTTATCAAACAACAATCTTTCCGGTGTCATACCAAGAAATCTATGTTCCAACACAACAAGTTTGGAGCACCTGATGATTTCGGATTCCAGAATTTTCGGCGAAATCCCAGCAGAATTAGGCCAATGCCGATCAATGAAGCAGCTCGACTTGTCCAATAACTTGCTCAACGGGTCAATCCCAGATGATATTTATGGACTGGTGGAGCTGACTGATCTATTGCTCCACAACAACAGCTTGGTGGGTTCGATTTCTCCAATGTTATCAAACCTCAGCAATCTGCAGTCACTTGTTCTGTACCACAATAATTTGGTGGGTCCTCTGCCGAACGAGATTGGAATGCTTGGGGAGCTTGAGATTCTGTATCTGTACGACAACCAGCTCTCCGGAGAAATCCCAATGGAGATTGGCAACTGTTCAAGCTTGCAGATGATCGATTTTTTCGGAAACCGGTTTAGTGGGGAGATTCCGATTACCATTGGGAGGCTCAAAGATTTGAACTTTCTTCATATCAGGCAGAACGAGCTGGTGGGTGAGATTCCCGCCCTATTGGGGAACTGTCACAAGTTGACTATTCTGGACTTGGCGGATAACCGTTTATCCGGTGGGATACCGATAACTTTCGGGTCCCTTCAAGCAATGGAGCAGTTCATGCTGTACAACAACTCGCTTGAAGGTAATCTTCCTGATACATTGGCTTATATGGCAAATTTGACTAGAGTGAATTTGTCGAGAAACAAGTTGAATGGGAGCATTGCTGCATTGTGTGGCTCGAGTTCGTTTCTTTCGTTTGATGTGACTGACAATGCATTTGATCATGAGATTCCTTCCCGGCTAGGAAATTCGAAATCTCTTGAAAGGTTGAGATTGGGGAAGAATCAGTTTACTGGTAAAATCCCATACGCATTGGGGAATATCAGTGAGCTATCACTGCTGGATGTGTCCGGAAATTCGCTCACTGGATCAATACCGGATGAGCTTTCATTCTGCATGAAACTGTCCCACATTGACTTGAACAACAACCTTCTTTCGGGCACAGTTCCGTGGTGGTTTGGAGGTTTGCCTCAGTTAGGAGAGCTGAAGCTGTCCTCGAATCGCTTCACCGGGCCTCTTCCTAATGAATTGTTCAATTGTTCTAAACTGCTAGTGCTTTCTATGAGTGAGAATTCTCTCAATGGAACCCTCCCTGCTGGGATTGGTAACCTGGAATTGCTTAACGTCCTAAACCTCGACCACAACCAGTTTTCGGGGCCAATTCCTCCAGCTATTGGGAAACTAGGCAAGCTGTATGAGCTTCAGCTCTCACAGAACAGATTTGATGGTGACATACCCTATGAGGTTGGACAACTGCAAAATCTTCAGAGCATTTTAGACCTCAGTTACAATAATTTGAGTGGTCAGATTCCAGCCTCTATTGGATCACTGTCGAAACTTGAAGCCCTCGATCTATCTCACAACCAACTTGTTGGAGAAGTTCCTTCCCCGGTTGGTGGGATGATCAGCTTGGGAAAGCTTAATCTCTCTTACAACAACCTACAGGGAAAGTTGAGCAAGCAACTCTCGCACTGGCCAGCTGATGCCTTCGCAGGAAACTTTCATCTTTGTGGAAGCCCTCTTGGAAGCTGCATTGGCAGGAAGAAGCAGACGGGGCCTAGAGAATCGGTGGTGGTAGCCATTGCTGCCATATGTACTCTATCTGCAATCGCTCTGCTGATATTTGGAGCTGCTTCTCTCTTGAAACACAAGCGAAAAGATTTTCGAAAAGCCAGTGACATGAACTGCCTGTACTCGACCAGCTCTTCCCATGCTCAGCGAAGACTGCTCTTCGCAAATAATTCTGTCAAGCCGGATATCAAATGGAAAGACATCATGGACGCCACGAAGAATCTGAGCAACGAGTTTGTAATTGGCTCAGGAGGGTCGGGAGTAATCTACAAAGCTGAACTGCGCACTGGAGAAACTGTGGCAGTAAAAAAGATTTTGTATAAAGACGATCTTATGGCAAATAAAAGCTTCACGACGGAGATTAAGACGCTTGGGAGGATAAGACACCGGCATCTGGTGAAGTTAATGGGGTACTGCAGCGACAAAGGAGCAGGCTCCAATCTGTTGATTTACGAGTACATGGAGAATGGAAGCGTGTATGATTGGATACACCAACAACAAAGTAACAAGAAGAAGAGCCTTGATTGGGAGGCGAGGCTAAAGATTGCGGTTGGATTAGCTCAAGGAGTGGAGTATCTCCACCATGACTGTGTGCCAAAGATCATTCACAGGGACATTAAGTCGAGCAATGTCCTGCTAGATTCCAACATGGAAGCACATCTTGGGGATTTTGGCCTTGCCAAGACACTTAACGAGACTTATGAGTCCAACACAGAATCGAATACATGGTTCGCTGGATCCTACGGTTACATAGCACCAGAGTATGCTTATTCACTCAAGGCATCAGAAAAGAGTGATGTCTACAGCATGGGGATTGTGCTTATGGAGCTTGTTAGCGGGAGAATGCCGACAGATTCATCTTTCGGCATGGACATAGACATGGTGAGATGGGTCGAGAGGCATATCGAAATGCAGGATTCTACTCGTGAAGCGTTGATAGACCCTGCACTGAAACCACTATTATCCGGAGAAGAAAATGCCGCATTCCAGGTTCTTGAAATTGCACTGCAGTGCACGAAAACCAGCCCAGCAGAGAGGCCTTCGTCACGACAGGCGTGTGATCAACTACTTCATGTCTTAAATCATCGGACGGTGGAGTTTGAGAAGAAGAATTTAGACTCTTATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1258

Amino Acids

136.51

Weight (kDa)

5.38

Isoelectric Point (pI)

35.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 72 4.3e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 266 - 377 1e-08 Leucine-rich repeat region
LRR_8 PF13855 298 - 357 5e-07 Leucine rich repeat
LRR_8 PF13855 347 - 406 1.3e-06 Leucine rich repeat
LRR_14 PF23598 437 - 569 7.2e-08 Leucine-rich repeat region
LRR_14 PF23598 480 - 706 1.2e-06 Leucine-rich repeat region
LRR_8 PF13855 515 - 574 4.1e-07 Leucine rich repeat
LRR_14 PF23598 706 - 782 2.8e-06 Leucine-rich repeat region
LRR_14 PF23598 749 - 836 1.2e-06 Leucine-rich repeat region
LRR_8 PF13855 754 - 814 1.3e-07 Leucine rich repeat
Pkinase PF00069 961 - 1235 6.4e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 961 - 1235 1.3e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014415)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20140
fragaria_vesca FvH4_3g13270 FvH4_3g13270 FvH4_3g13270
malus_domestica MD05G1243600.v1.1 MD10G1225500.v1.1
prunus_persica Prupe.4G120100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0021741
rosa_laevigata RLG00000032624
rosa_multiflora Rmu_ssc0000233.1_g000008
rosa_roxburghii Rroxscaffold_1G00056980
rosa_rugosa Rorug05G0068900
rosa_samantha Rh5AG157400 Rh5BG157100 Rh5CG171200 Rh5DG157300
rosa_wichuraiana Rw5G014030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 239, 3346
Acc16I TGCGCA 1 cut(s) 2927
Acc36I ACCTGC 1 cut(s) 327
AccB1I GGYRCC 1 cut(s) 953
AccB7I CCANNNNNTGG 2 cut(s) 250, 2246
AccI GTMKAC 1 cut(s) 3444
AccII CGCG 1 cut(s) 728
AccIII TCCGGA 3 cut(s) 1364, 1922, 3608
AclWI GGATC 4 cut(s) 1948, 2404, 3375, 3388
AcoI YGGCCR 5 cut(s) 592, 654, 736, 787, 2546
AcuI CTGAAG 4 cut(s) 881, 2078, 2258, 2321
AcyI GRCGYC 1 cut(s) 2853
AfaI GTAC 9 cut(s) 196, 574, 1277, 1349, 1637, 2668, 2765, 3056, 3101
AgeI ACCGGT 1 cut(s) 1428
AhdI GACNNNNNGTC 1 cut(s) 3262
AjiI CACGTC 1 cut(s) 387
AjnI CCWGG 2 cut(s) 2181, 3630
AjuI GAANNNNNNNTTGG 2 cut(s) 3288, 3320
AleI CACNNNNGTG 1 cut(s) 3652
Alw21I GWGCWC 4 cut(s) 735, 759, 1050, 3659
Alw26I GTCTC 4 cut(s) 444, 467, 746, 3332
Alw44I GTGCAC 1 cut(s) 3655
AlwI GGATC 4 cut(s) 1948, 2404, 3375, 3388
Ama87I CYCGRG 4 cut(s) 352, 638, 752, 1750
Aor13HI TCCGGA 3 cut(s) 1364, 1922, 3608
ApaLI GTGCAC 1 cut(s) 3655
ApeKI GCWGC 9 cut(s) 561, 625, 695, 1120, 1736, 2601, 2657, 2702, 3060
ArsI GACNNNNNNTTYG 4 cut(s) 784, 816, 1772, 1804
AsiGI ACCGGT 1 cut(s) 1428
Asp700I GAANNNNTTC 1 cut(s) 2075
AspLEI GCGC 1 cut(s) 2928
AsuC2I CCSGG 4 cut(s) 283, 1810, 2083, 2462
AsuHPI GGTGA 7 cut(s) 136, 821, 1523, 2071, 2312, 3052, 3538
AsuII TTCGAA 2 cut(s) 1823, 2737
AvaI CYCGRG 4 cut(s) 352, 638, 752, 1750
AvaII GGWCC 9 cut(s) 171, 241, 356, 428, 501, 644, 860, 1295, 1605
BaeGI GKGCMC 2 cut(s) 2015, 3659
BalI TGGCCA 2 cut(s) 789, 2548
BamHI GGATCC 1 cut(s) 3380
BanI GGYRCC 1 cut(s) 953
BanII GRGCYC 4 cut(s) 258, 495, 735, 759
BauI CACGAG 1 cut(s) 3567
BbsI GAAGAC 2 cut(s) 150, 2800
Bbv12I GWGCWC 4 cut(s) 735, 759, 1050, 3659
BbvCI CCTCAGC 1 cut(s) 1250
BbvI GCAGC 9 cut(s) 573, 637, 682, 1132, 1723, 2588, 2644, 2689, 3072
BccI CCATC 4 cut(s) 162, 368, 2291, 3525
BceAI ACGGC 2 cut(s) 361, 723
BciT130I CCWGG 2 cut(s) 2183, 3632
BciVI GTATCC 2 cut(s) 1581, 3123
BclI TGATCA 3 cut(s) 1792, 2475, 3703
BcnI CCSGG 4 cut(s) 283, 1810, 2083, 2462
BcoDI GTCTC 4 cut(s) 444, 467, 746, 3332
BfaI CTAG 6 cut(s) 1695, 1814, 2123, 2255, 2631, 3281
BfmI CTRYAG 6 cut(s) 623, 1259, 2513, 3058, 3445, 3650
BfuAI ACCTGC 1 cut(s) 327
BfuI GTATCC 2 cut(s) 1581, 3123
BglI GCCNNNNNGGC 2 cut(s) 600, 786
BlpI GCTNAGC 1 cut(s) 2787
Bme1390I CCNGG 6 cut(s) 283, 1810, 2083, 2183, 2462, 3632
Bme18I GGWCC 9 cut(s) 171, 241, 356, 428, 501, 644, 860, 1295, 1605
BmeRI GACNNNNNGTC 1 cut(s) 3262
BmeT110I CYCGRG 4 cut(s) 352, 638, 752, 1750
BmgBI CACGTC 1 cut(s) 387
BmrFI CCNGG 6 cut(s) 283, 1810, 2083, 2183, 2462, 3632
BmrI ACTGGG 2 cut(s) 507, 896
BmsI GCATC 5 cut(s) 7, 61, 2545, 3043, 3434
BmuI ACTGGG 2 cut(s) 507, 896
BpiI GAAGAC 2 cut(s) 150, 2800
BpmI CTGGAG 3 cut(s) 629, 2223, 2952
Bpu10I CCTNAGC 2 cut(s) 1250, 2890
Bpu1102I GCTNAGC 1 cut(s) 2787
Bpu14I TTCGAA 2 cut(s) 1823, 2737
BpuEI CTTGAG 3 cut(s) 1352, 3189, 3404
BpuMI CCSGG 4 cut(s) 283, 1810, 2083, 2462
Bsa29I ATCGAT 1 cut(s) 1413
BsaHI GRCGYC 1 cut(s) 2853
BsaJI CCNNGG 4 cut(s) 390, 726, 2021, 2460
BsaWI WCCGGW 7 cut(s) 1001, 1364, 1428, 1580, 1922, 1948, 3608
BsaXI ACNNNNNCTCC 6 cut(s) 565, 595, 1346, 1376, 2925, 2955
Bse118I RCCGGY 2 cut(s) 1428, 3030
Bse3DI GCAATG 4 cut(s) 1623, 1731, 2652, 3277
BseAI TCCGGA 3 cut(s) 1364, 1922, 3608
BseBI CCWGG 2 cut(s) 2183, 3632
BseCI ATCGAT 1 cut(s) 1413
BseDI CCNNGG 4 cut(s) 390, 726, 2021, 2460
BseGI GGATG 5 cut(s) 173, 360, 1921, 1957, 2478
BseMI GCAATG 4 cut(s) 1623, 1731, 2652, 3277
BseRI GAGGAG 1 cut(s) 948
BseSI GKGCMC 2 cut(s) 2015, 3659
BseX3I CGGCCG 3 cut(s) 592, 654, 736
BseXI GCAGC 9 cut(s) 573, 637, 682, 1132, 1723, 2588, 2644, 2689, 3072
BseYI CCCAGC 4 cut(s) 963, 1087, 2168, 3670
BsgI GTGCAG 1 cut(s) 3573
Bsh1236I CGCG 1 cut(s) 728
Bsh1285I CGRYCG 3 cut(s) 595, 657, 739
BshNI GGYRCC 1 cut(s) 953
BshTI ACCGGT 1 cut(s) 1428
BshVI ATCGAT 1 cut(s) 1413
BsiEI CGRYCG 3 cut(s) 595, 657, 739
BsiHKAI GWGCWC 4 cut(s) 735, 759, 1050, 3659
BsiHKCI CYCGRG 4 cut(s) 352, 638, 752, 1750
BslFI GGGAC 6 cut(s) 342, 851, 946, 1591, 1963, 3269
BsmAI GTCTC 4 cut(s) 444, 467, 746, 3332
BsmBI CGTCTC 2 cut(s) 444, 746
BsmFI GGGAC 6 cut(s) 342, 851, 946, 1591, 1963, 3269
BsmI GAATGC 3 cut(s) 1323, 3492, 3626
BsoBI CYCGRG 4 cut(s) 352, 638, 752, 1750
Bsp119I TTCGAA 2 cut(s) 1823, 2737
Bsp1286I GDGCHC 7 cut(s) 258, 495, 735, 759, 1050, 2015, 3659
Bsp13I TCCGGA 3 cut(s) 1364, 1922, 3608
Bsp1407I TGTACA 1 cut(s) 1635
Bsp1720I GCTNAGC 1 cut(s) 2787
BspDI ATCGAT 1 cut(s) 1413
BspEI TCCGGA 3 cut(s) 1364, 1922, 3608
BspFNI CGCG 1 cut(s) 728
BspHI TCATGA 2 cut(s) 471, 1795
BspMAI CTGCAG 4 cut(s) 627, 1263, 3062, 3654
BspMI ACCTGC 1 cut(s) 327
BspPI GGATC 4 cut(s) 1948, 2404, 3375, 3388
BspQI GCTCTTC 3 cut(s) 2782, 2807, 3152
BspT104I TTCGAA 2 cut(s) 1823, 2737
BspT107I GGYRCC 1 cut(s) 953
BsrDI GCAATG 4 cut(s) 1623, 1731, 2652, 3277
BsrFI RCCGGY 2 cut(s) 1428, 3030
BsrGI TGTACA 1 cut(s) 1635
BssAI RCCGGY 2 cut(s) 1428, 3030
BssECI CCNNGG 4 cut(s) 390, 726, 2021, 2460
BssNI GRCGYC 1 cut(s) 2853
BssSI CACGAG 1 cut(s) 3567
BssT1I CCWWGG 1 cut(s) 390
Bst2BI CACGAG 1 cut(s) 3567
Bst2UI CCWGG 2 cut(s) 2183, 3632
Bst6I CTCTTC 5 cut(s) 122, 2094, 2782, 2807, 3152
BstACI GRCGYC 1 cut(s) 2853
BstAPI GCANNNNNTGC 2 cut(s) 319, 1630
BstAUI TGTACA 1 cut(s) 1635
BstBI TTCGAA 2 cut(s) 1823, 2737
BstC8I GCNNGC 7 cut(s) 452, 969, 1403, 2261, 2531, 2550, 3076
BstDSI CCRYGG 2 cut(s) 726, 2021
BstEII GGTNACC 1 cut(s) 2177
BstENI CCTNNNNNAGG 1 cut(s) 2046
BstF5I GGATG 5 cut(s) 173, 360, 1921, 1957, 2478
BstFNI CGCG 1 cut(s) 728
BstHHI GCGC 1 cut(s) 2928
BstMAI GTCTC 4 cut(s) 444, 467, 746, 3332
BstMCI CGRYCG 3 cut(s) 595, 657, 739
BstNI CCWGG 2 cut(s) 2183, 3632
BstPI GGTNACC 1 cut(s) 2177
BstSCI CCNGG 6 cut(s) 281, 1808, 2081, 2181, 2460, 3630
BstSFI CTRYAG 6 cut(s) 623, 1259, 2513, 3058, 3445, 3650
BstSLI GKGCMC 2 cut(s) 2015, 3659
BstUI CGCG 1 cut(s) 728
BstV1I GCAGC 9 cut(s) 573, 637, 682, 1132, 1723, 2588, 2644, 2689, 3072
BstV2I GAAGAC 2 cut(s) 150, 2800
BstX2I RGATCY 1 cut(s) 3380
BstXI CCANNNNNNTGG 2 cut(s) 425, 2444
BstYI RGATCY 1 cut(s) 3380
BstZI CGGCCG 3 cut(s) 592, 654, 736
Bsu15I ATCGAT 1 cut(s) 1413
BsuI GTATCC 2 cut(s) 1581, 3123
BsuTUI ATCGAT 1 cut(s) 1413
BtgI CCRYGG 2 cut(s) 726, 2021
BtrI CACGTC 1 cut(s) 387
BtsCI GGATG 5 cut(s) 173, 360, 1921, 1957, 2478
BtsI GCAGTG 3 cut(s) 1907, 3647, 3659
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 7 cut(s) 452, 969, 1403, 2261, 2531, 2550, 3076
CciI TCATGA 2 cut(s) 471, 1795
CfoI GCGC 1 cut(s) 2928
Cfr10I RCCGGY 2 cut(s) 1428, 3030
Cfr42I CCGCGG 1 cut(s) 729
ClaI ATCGAT 1 cut(s) 1413
CseI GACGC 2 cut(s) 2861, 3019
Csp6I GTAC 9 cut(s) 195, 573, 1276, 1348, 1636, 2667, 2764, 3055, 3100
CspAI ACCGGT 1 cut(s) 1428
CviQI GTAC 9 cut(s) 195, 573, 1276, 1348, 1636, 2667, 2764, 3055, 3100
DrdI GACNNNNNNGTC 2 cut(s) 239, 3346
DriI GACNNNNNGTC 1 cut(s) 3262
DseDI GACNNNNNNGTC 2 cut(s) 239, 3346
EaeI YGGCCR 5 cut(s) 592, 654, 736, 787, 2546
EagI CGGCCG 3 cut(s) 592, 654, 736
Eam1104I CTCTTC 5 cut(s) 122, 2094, 2782, 2807, 3152
Eam1105I GACNNNNNGTC 1 cut(s) 3262
EarI CTCTTC 5 cut(s) 122, 2094, 2782, 2807, 3152
EciI GGCGGA 1 cut(s) 1580
Ecl136II GAGCTC 2 cut(s) 733, 757
EclXI CGGCCG 3 cut(s) 592, 654, 736
Eco130I CCWWGG 1 cut(s) 390
Eco147I AGGCCT 1 cut(s) 3682
Eco24I GRGCYC 4 cut(s) 258, 495, 735, 759
Eco32I GATATC 2 cut(s) 291, 2830
Eco47I GGWCC 9 cut(s) 171, 241, 356, 428, 501, 644, 860, 1295, 1605
Eco52I CGGCCG 3 cut(s) 592, 654, 736
Eco53kI GAGCTC 2 cut(s) 733, 757
Eco57I CTGAAG 4 cut(s) 881, 2078, 2258, 2321
Eco88I CYCGRG 4 cut(s) 352, 638, 752, 1750
Eco91I GGTNACC 1 cut(s) 2177
EcoICRI GAGCTC 2 cut(s) 733, 757
EcoNI CCTNNNNNAGG 1 cut(s) 2046
EcoO109I RGGNCCY 4 cut(s) 356, 1295, 1605, 2627
EcoO65I GGTNACC 1 cut(s) 2177
EcoRI GAATTC 1 cut(s) 2143
EcoRII CCWGG 2 cut(s) 2181, 3630
EcoRV GATATC 2 cut(s) 291, 2830
EcoT14I CCWWGG 1 cut(s) 390
EcoT22I ATGCAT 2 cut(s) 1789, 3772
EcoT38I GRGCYC 4 cut(s) 258, 495, 735, 759
ErhI CCWWGG 1 cut(s) 390
Esp3I CGTCTC 2 cut(s) 444, 746
FalI AAGNNNNNCTT 6 cut(s) 1644, 1676, 2958, 2990, 3288, 3320
FaqI GGGAC 6 cut(s) 342, 851, 946, 1591, 1963, 3269
FauI CCCGC 5 cut(s) 372, 517, 733, 1528, 3473
FauNDI CATATG 1 cut(s) 2663
FbaI TGATCA 3 cut(s) 1792, 2475, 3703
FblI GTMKAC 1 cut(s) 3444
FokI GGATG 5 cut(s) 180, 347, 1928, 1964, 2485
FriOI GRGCYC 4 cut(s) 258, 495, 735, 759
FspBI CTAG 6 cut(s) 1695, 1814, 2123, 2255, 2631, 3281
FspI TGCGCA 1 cut(s) 2927
GlaI GCGC 1 cut(s) 2927
GsaI CCCAGC 4 cut(s) 967, 1091, 2172, 3674
GsuI CTGGAG 3 cut(s) 629, 2223, 2952
HgaI GACGC 2 cut(s) 2861, 3019
HhaI GCGC 1 cut(s) 2928
Hin1I GRCGYC 1 cut(s) 2853
Hin6I GCGC 1 cut(s) 2926
HinP1I GCGC 1 cut(s) 2926
HincII GTYRAC 3 cut(s) 803, 821, 1548
HindII GTYRAC 3 cut(s) 803, 821, 1548
HindIII AAGCTT 3 cut(s) 1399, 2489, 2989
HphI GGTGA 7 cut(s) 136, 821, 1523, 2071, 2312, 3052, 3538
Hpy166II GTNNAC 9 cut(s) 232, 428, 803, 812, 821, 1548, 1863, 3445, 3657
Hpy8I GTNNAC 9 cut(s) 232, 428, 803, 812, 821, 1548, 1863, 3445, 3657
Hpy99I CGWCG 3 cut(s) 95, 947, 3001
HpyAV CCTTC 8 cut(s) 851, 1619, 1646, 1815, 2012, 2466, 2569, 3693
HpyCH4IV ACGT 3 cut(s) 386, 945, 2196
HpySE526I ACGT 3 cut(s) 386, 945, 2196
Hsp92I GRCGYC 1 cut(s) 2853
HspAI GCGC 1 cut(s) 2926
KflI GGGWCCC 2 cut(s) 356, 1605
Kpn2I TCCGGA 3 cut(s) 1364, 1922, 3608
Ksp22I TGATCA 3 cut(s) 1792, 2475, 3703
KspI CCGCGG 1 cut(s) 729
LguI GCTCTTC 3 cut(s) 2782, 2807, 3152
Lsp1109I GCAGC 9 cut(s) 573, 637, 682, 1132, 1723, 2588, 2644, 2689, 3072
LweI GCATC 5 cut(s) 7, 61, 2545, 3043, 3434
MaeI CTAG 6 cut(s) 1695, 1814, 2123, 2255, 2631, 3281
MaeII ACGT 3 cut(s) 386, 945, 2196
MfeI CAATTG 2 cut(s) 135, 2107
MflI RGATCY 1 cut(s) 3380
MhlI GDGCHC 7 cut(s) 258, 495, 735, 759, 1050, 2015, 3659
MlsI TGGCCA 2 cut(s) 789, 2548
MluNI TGGCCA 2 cut(s) 789, 2548
MlyI GAGTC 9 cut(s) 218, 263, 469, 477, 591, 826, 910, 3356, 3755
MmeI TCCRAC 7 cut(s) 291, 1053, 2299, 2425, 3175, 3312, 3375
Mox20I TGGCCA 2 cut(s) 789, 2548
Mph1103I ATGCAT 2 cut(s) 1789, 3772
MroI TCCGGA 3 cut(s) 1364, 1922, 3608
MroXI GAANNNNTTC 1 cut(s) 2075
MscI TGGCCA 2 cut(s) 789, 2548
MseI TTAA 8 cut(s) 617, 2193, 2493, 3006, 3047, 3261, 3333, 3722
MslI CAYNNNNRTG 4 cut(s) 2580, 3231, 3521, 3652
Msp20I TGGCCA 2 cut(s) 789, 2548
MspA1I CMGCKG 4 cut(s) 695, 728, 967, 2552
MspR9I CCNGG 6 cut(s) 283, 1810, 2083, 2183, 2462, 3632
MunI CAATTG 2 cut(s) 135, 2107
Mva1269I GAATGC 3 cut(s) 1323, 3492, 3626
MvaI CCWGG 2 cut(s) 2183, 3632
MvnI CGCG 1 cut(s) 728
NciI CCSGG 4 cut(s) 283, 1810, 2083, 2462
NdeI CATATG 1 cut(s) 2663
NmeAIII GCCGAG 5 cut(s) 426, 570, 579, 682, 714
NmuCI GTSAC 9 cut(s) 208, 273, 538, 1263, 1538, 1774, 2300, 2747, 3687
NsbI TGCGCA 1 cut(s) 2927
NsiI ATGCAT 2 cut(s) 1789, 3772
NspV TTCGAA 2 cut(s) 1823, 2737
OliI CACNNNNGTG 1 cut(s) 3652
PaeR7I CTCGAG 3 cut(s) 638, 752, 1750
PagI TCATGA 2 cut(s) 471, 1795
PceI AGGCCT 1 cut(s) 3682
PciSI GCTCTTC 3 cut(s) 2782, 2807, 3152
PcsI WCGNNNNNNNCGW 1 cut(s) 1763
PctI GAATGC 3 cut(s) 1323, 3492, 3626
PdmI GAANNNNTTC 1 cut(s) 2075
PflMI CCANNNNNTGG 2 cut(s) 250, 2246
PinAI ACCGGT 1 cut(s) 1428
PleI GAGTC 9 cut(s) 218, 263, 468, 476, 591, 825, 909, 3355, 3755
PpsI GAGTC 9 cut(s) 218, 263, 468, 476, 591, 825, 909, 3355, 3755
PpuMI RGGWCCY 3 cut(s) 356, 1295, 1605
Psp124BI GAGCTC 2 cut(s) 735, 759
Psp5II RGGWCCY 3 cut(s) 356, 1295, 1605
Psp6I CCWGG 2 cut(s) 2181, 3630
PspEI GGTNACC 1 cut(s) 2177
PspFI CCCAGC 4 cut(s) 963, 1087, 2168, 3670
PspGI CCWGG 2 cut(s) 2181, 3630
PspPPI RGGWCCY 3 cut(s) 356, 1295, 1605
PspXI VCTCGAGB 3 cut(s) 638, 752, 1750
PsrI GAACNNNNNNTAC 2 cut(s) 2747, 2779
PstI CTGCAG 4 cut(s) 627, 1263, 3062, 3654
PsuI RGATCY 1 cut(s) 3380
PvuII CAGCTG 3 cut(s) 695, 967, 2552
RsaI GTAC 9 cut(s) 196, 574, 1277, 1349, 1637, 2668, 2765, 3056, 3101
RsaNI GTAC 9 cut(s) 195, 573, 1276, 1348, 1636, 2667, 2764, 3055, 3100
RseI CAYNNNNRTG 4 cut(s) 2580, 3231, 3521, 3652
SacI GAGCTC 2 cut(s) 735, 759
SacII CCGCGG 1 cut(s) 729
SapI GCTCTTC 3 cut(s) 2782, 2807, 3152
SaqAI TTAA 8 cut(s) 617, 2193, 2493, 3006, 3047, 3261, 3333, 3722
SchI GAGTC 9 cut(s) 218, 263, 469, 477, 591, 826, 910, 3356, 3755
ScrFI CCNGG 6 cut(s) 283, 1810, 2083, 2183, 2462, 3632
SduI GDGCHC 7 cut(s) 258, 495, 735, 759, 1050, 2015, 3659
SfaNI GCATC 5 cut(s) 7, 61, 2545, 3043, 3434
SfcI CTRYAG 6 cut(s) 623, 1259, 2513, 3058, 3445, 3650
SfiI GGCCNNNNNGGCC 1 cut(s) 600
Sfr274I CTCGAG 3 cut(s) 638, 752, 1750
Sfr303I CCGCGG 1 cut(s) 729
SfuI TTCGAA 2 cut(s) 1823, 2737
SgrBI CCGCGG 1 cut(s) 729
SinI GGWCC 9 cut(s) 171, 241, 356, 428, 501, 644, 860, 1295, 1605
SlaI CTCGAG 3 cut(s) 638, 752, 1750
SmiMI CAYNNNNRTG 4 cut(s) 2580, 3231, 3521, 3652
SmlI CTYRAG 6 cut(s) 638, 752, 1331, 1750, 3204, 3419
SmoI CTYRAG 6 cut(s) 638, 752, 1331, 1750, 3204, 3419
SseBI AGGCCT 1 cut(s) 3682
SspMI CTAG 6 cut(s) 1695, 1814, 2123, 2255, 2631, 3281
SstI GAGCTC 2 cut(s) 735, 759
StuI AGGCCT 1 cut(s) 3682
StyD4I CCNGG 6 cut(s) 281, 1808, 2081, 2181, 2460, 3630
StyI CCWWGG 1 cut(s) 390
TaiI ACGT 3 cut(s) 389, 948, 2199
TaqII GACCGA 1 cut(s) 229
TatI WGTACW 5 cut(s) 194, 1635, 2666, 2763, 3099
TauI GCSGC 2 cut(s) 31, 3626
Tru1I TTAA 8 cut(s) 617, 2193, 2493, 3006, 3047, 3261, 3333, 3722
Tru9I TTAA 8 cut(s) 617, 2193, 2493, 3006, 3047, 3261, 3333, 3722
TseFI GTSAC 9 cut(s) 208, 273, 538, 1263, 1538, 1774, 2300, 2747, 3687
TseI GCWGC 9 cut(s) 561, 625, 695, 1120, 1736, 2601, 2657, 2702, 3060
Tsp45I GTSAC 9 cut(s) 208, 273, 538, 1263, 1538, 1774, 2300, 2747, 3687
TspGWI ACGGA 5 cut(s) 108, 426, 729, 2010, 3014
Van91I CCANNNNNTGG 2 cut(s) 250, 2246
VneI GTGCAC 1 cut(s) 3655
VpaK11BI GGWCC 9 cut(s) 171, 241, 356, 428, 501, 644, 860, 1295, 1605
XagI CCTNNNNNAGG 1 cut(s) 2046
XcmI CCANNNNNNNNNTGG 2 cut(s) 883, 964
XhoI CTCGAG 3 cut(s) 638, 752, 1750
XmiI GTMKAC 1 cut(s) 3444
XmnI GAANNNNTTC 1 cut(s) 2075
XspI CTAG 6 cut(s) 1695, 1814, 2123, 2255, 2631, 3281
Zsp2I ATGCAT 2 cut(s) 1789, 3772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.