MD10G1225500.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014415
Physical Location & Seq
Reverse (-)
32283607 .. 32287692
4086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1225500.v1.1.491

Sequence Viewer

Length: 3693 bp
ATGGCTTTAGTAAGAGATGCAATTTTGCCTCTTTTTCTTGCTCTGTTTCTGCATCTTTCATTAGGGTTTATGTTCTGCGAGGGGAAGCTCGATGGCAATACAACTTTGAGTGTGCTTTTGGAGGTGAAGAGGGCAATTGTGGAAGACCCAGAAAATGTTTTGCATGGCTGGTCCGAAAGCGACCCAAATTTCTGTACTTGGAGAGGTGTGACTTGTGCGCTTGACTCGCTGGACAACTCGGTCCAAGCGGTAGGGCTCAACCTTTCCGGCTCGTCACTCGCCGGGTCGATATCGCCCTCACTCGGTAGTTTGCAAAACCTGCTCCACCTTGATCTCTCTTCTAACCGCCTCTCGGGTCCCATCCCACCAACCCTCTCTAACCTCACGTCCTTGGAATCTTTGCTTCTCTTTTCAAACCAGCTCACTGGGGACATCCCGAATCATCTCAGCTCGCTGACGAGTCTCCAAGTGATGAGAATCGGCGACAATGGGCTCACTGGATCCATTCCCGCCACTTTTGGCAAACTTGTCAATTTGGTCACGCTTGGTTTGGCCTCATGCAGCCTCAACGGTCCAATACCTCCCCAACTCGGCCGACTCGGCCTACTGGAGAACTTAATCCTGCAGCTCAATCAACTCGAAGGTTTGATTCCTGCTGAGTTGGGGAACTGCTCCAGCCTCACTATCTTCACAGCTGCCAACAATACCCTCAACGGAACAATCCCCGCGCAGCTCGGCCGTCTCGGTAACCTCCAGCTCCTGAACTTGGGAAACAACAGCCTCACCGGCCAAATCCCGAGTCAACTCGGTAAACTGAGTCAACTCGGATACATGAATCTCATGGGGAACCAACTTGAAGGTCCAATACCAAAGTCCATATCTCAACTGGGAAATCTTCAGAGTCTTGATTTATCCGTGAACAAGCTCACAGGAGGTATTCCGGCCGAATTTGGCACCATGTCTCAGCTGGCAACCTTAGTTTTATCAAACAACAGTCTTTCCGGCGTCATCCCAAGAAATCTATGTTCCAACACAACAAGTTTGGAGCTCCTAATGATTTCAGGTTCGGGACTTGTCGGCGAGATCCCAGCAGAATTAAGCCAATGCCGATCAATGAAGCAGCTCGACTTGTCCAATAACTTGATCAACGGGTCAATCCCAGATGAGATTTATGGACTAGTGGAGCTGACTGATCTGTTGCTCCACAACAACAGCTTGGTTGGTTCGATTTCTCCGTTGATATCAAACCTCAGCAATCTGCAGTCACTTATTCTGTACCATAACAACTTGGTGGGCCCTCTGCCGAGCGAGATTGGAATGCTTGGGGAGCTCGAGATTCTATATCTCTACGACAATCAGCTCTCTGGAGAAATCCCAATGGAGATTGGCAACTGTTCGAGCTTGCAGATGATCGATTTTTTTGGAAATCGGTTCAGCGGGGAGATCCCGGTTACTATTGGGAGGCTCAAAGATTTGAACTTACTTCATATCAGGCAGAATGAGTTGGTGGGTGAGATTCCCGCCACATTGGGGAACTGTCACAAGCTGACTATTCTTGACTTGGCGGATAACCGTTTATCTGGCGGGATACCGATAACTTTCGGGTCCCTTGGAGCTATGGAGCAGTTCATGCTGTACAACAACTCACTTGAAGGTAATCTTCCTGATACATTGGCTAATATGGCGAATTTGACTAGAGTGAATTTATCGGGAAACAAGTTGAATGGAAGCATTGCTGCATTGTGTAGCTCCAGTTCGTTTCTTTCGTTTGATGTCACTGACAATGCATTTGATCATGAGATTCCTTCCCAGCTCGGAAATTCGAAATCTCTCCAGAGGTTGAGATTAGGGAACAATCAGTTTACTGGTAAAATCCCACACACATTGGGGAATATCAGTGAGCTATCACTGCTAGATGTGTCCGGAAATTCACTCACAGGATCAATACCGGACGAGCTTTCATCGTGCAAGAAACTGTCCCACATTGACTTGAACGACAACCTTCTTTCAGGCACAATTCCGCAGTGGTTTGGAGGTTTGCCTCAGTTAGGAGAGCTGAAGCTGTCCTCAAATCGCTTCACCGGGGCTCTTCCGATTGAATTGTTCAATTGTTCTAAACTGCTAGTGCTTTCCATGAGTGAGAATTCTCTCATTGGAACCCTCCCTGCTGAGATTGGTAACCTGGAATCGCTTAATGTCCTAAACCTCGACCACAACCATTTTTCAGGTCCAATCCCTCCTGCCATTGGGAAACTAGGCAAGCTGTATGAGCTTCGGCTCTCACAGAACAGATTTGATGGTGACTTGCCTTATGAGCTTGGACAACTCCAAAATCTTCAGAGCATTTTAGACCTTAGTTACAACAATTTGAGTGGTCAGATTCCGGCCTCTATTGGGACTCTGTCAAAACTTGAAGTGCTTGATCTATCTCACAATCAACTAATTGGCGAAGTCCCTTCCCCAGTTGGTGGGATGATTAGCTTGGGAAAGCTTAATCTGTCTTACAACAATCTGCAAGGAAAGTTGAGCAAGCAACTCTCGCACTGGCCGGCTGAGGCTTTTGAGGGAAACTTGGATCTTTGTGGAAGCCCTCTTGGAAGCTGCATTGGCAGAAAGCAGCAGTCTGATCCAACAGAATTGGCAGTGGTAGCCCTTGCTGCAATATGTACTCTATCTGCAATTGCTCTACTGATATTCGGAGCTGCTTCTCTCCTGAAACACAAGCGAGAAGCTTTTCGGAAAGCCCGTGAAATGAACTTCTTGTACTCGTCCAGTTCTTCCCATGCTCAGCGAAGACTGCTTTTCTCAAACGGTTCTGTCAAGCCAGATATCAAATGGAAAGACATCATGGACGCCACAAAGAATCTGAGCAACGAATTTGTAATTGGCTCAGGAGGGTCGGGAGTAATCTACAAAGCTGAACTGCGAACAGGAGAAACTGTGGCAGTCAAGAAGATTTTGTATAAAGATGATCTTATGGCGAACAAAACCTTCACTAGGGAGATTAAGACACTTGGGAGGATAAGACACCGGCATCTGGTGAAGTTAATGGGGTACTGCAGCAACAAAGGAGCAGGATCCAATCTGTTAATATACGAGTACATGGAGAACGGAAGTGTGTATGATTGGATACACCAAGAGCAAAGTAACAAGAAGAAGAAGAGCCTTGATTGGGAGGCACGGCTAAAGATTGCGGTTGGATTAGCTCAAGGAGTGGAGTATCTCCACCATGACTGCGTGCCAAAGATCATCCATAGGGACATTAAGTCGAGCAATATTTTGCTAGATTCGAACATGGAGGCACATCTTGGGGATTTCGGCCTTGCCAAGACACTTAACGAGAACAACGAGTCTAACACAGAATCTAATACATGGTTTGCTGGATCCTACGGGTACATAGCACCAGAGTATGCTTATTCACTCAAGGCATCAGAAAAGAGTGATGTCTACAGCATGGGGATTGTGCTAATGGAGCTTGATTCTACTCGTGAAGAGTTGATAGACCCTGCACTGAAACCACTCTTGTCCGGAGAAGAAAACACCGCGTTCCAGGTTCTTGAAATTGCACTGCAGTGCACAAAAACCAGCCCGGCAGAGAGGCCTTCGTCACGAGAAGCGTGTGATCAACTACTACATGTGTTTAATCATCGGTCGGTGGAGTTTGAGAAGACGAATTTAGATTCTTACACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1231

Amino Acids

133.08

Weight (kDa)

5.38

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 72 2.5e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 226 - 377 7.8e-10 Leucine-rich repeat region
LRR_14 PF23598 365 - 473 4.9e-06 Leucine-rich repeat region
LRR_8 PF13855 515 - 574 6.4e-07 Leucine rich repeat
LRR_8 PF13855 654 - 693 1e-06 Leucine rich repeat
LRR_14 PF23598 708 - 784 3.6e-08 Leucine-rich repeat region
LRR_8 PF13855 754 - 814 1.3e-06 Leucine rich repeat
Pkinase PF00069 961 - 1192 1.3e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 961 - 1205 5.7e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014415)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20140
fragaria_vesca FvH4_3g13270 FvH4_3g13270 FvH4_3g13270
malus_domestica MD05G1243600.v1.1 MD10G1225500.v1.1
prunus_persica Prupe.4G120100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0021741
rosa_laevigata RLG00000032624
rosa_multiflora Rmu_ssc0000233.1_g000008
rosa_roxburghii Rroxscaffold_1G00056980
rosa_rugosa Rorug05G0068900
rosa_samantha Rh5AG157400 Rh5BG157100 Rh5CG171200 Rh5DG157300
rosa_wichuraiana Rw5G014030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
Acc36I ACCTGC 1 cut(s) 327
AccB1I GGYRCC 1 cut(s) 953
AccB7I CCANNNNNTGG 1 cut(s) 2468
AccI GTMKAC 1 cut(s) 3447
AccII CGCG 2 cut(s) 728, 3545
AccIII TCCGGA 2 cut(s) 1922, 3527
AcoI YGGCCR 5 cut(s) 592, 736, 787, 942, 2546
AcsI RAATTY 9 cut(s) 187, 947, 1687, 1702, 1819, 1927, 2143, 2876, 3673
AcuI CTGAAG 3 cut(s) 881, 2078, 2321
AcyI GRCGYC 2 cut(s) 1005, 2853
AfaI GTAC 8 cut(s) 196, 1277, 1637, 2668, 2765, 3056, 3101, 3395
AflIII ACRYGT 1 cut(s) 3634
AhdI GACNNNNNGTC 1 cut(s) 3265
AhlI ACTAGT 1 cut(s) 1177
AjiI CACGTC 1 cut(s) 387
AjnI CCWGG 2 cut(s) 2181, 3549
AjuI GAANNNNNNNTTGG 2 cut(s) 2868, 2900
AleI CACNNNNGTG 1 cut(s) 3571
Alw21I GWGCWC 3 cut(s) 1050, 1332, 3578
Alw26I GTCTC 3 cut(s) 467, 746, 966
Alw44I GTGCAC 1 cut(s) 3574
AlwNI CAGNNNCTG 1 cut(s) 760
Ama87I CYCGRG 3 cut(s) 352, 796, 1331
Aor13HI TCCGGA 2 cut(s) 1922, 3527
ApaI GGGCCC 1 cut(s) 1298
ApaLI GTGCAC 1 cut(s) 3574
ApoI RAATTY 9 cut(s) 187, 947, 1687, 1702, 1819, 1927, 2143, 2876, 3673
ArsI GACNNNNNNTTYG 4 cut(s) 784, 816, 1772, 1804
Asp700I GAANNNNTTC 1 cut(s) 2733
AspLEI GCGC 2 cut(s) 220, 730
AspS9I GGNCC 9 cut(s) 171, 241, 356, 572, 860, 1294, 1295, 1605, 2228
AsuC2I CCSGG 4 cut(s) 283, 1448, 2083, 3590
AsuHPI GGTGA 6 cut(s) 136, 775, 1523, 2071, 2312, 3052
AsuII TTCGAA 2 cut(s) 1823, 3290
AvaI CYCGRG 3 cut(s) 352, 796, 1331
AvaII GGWCC 7 cut(s) 171, 241, 356, 572, 860, 1605, 2228
BaeGI GKGCMC 2 cut(s) 1298, 3578
BamHI GGATCC 3 cut(s) 500, 3077, 3383
BanI GGYRCC 1 cut(s) 953
BanII GRGCYC 6 cut(s) 258, 495, 1050, 1298, 1332, 2089
BarI GAAGNNNNNNTAC 2 cut(s) 849, 881
BauI CACGAG 2 cut(s) 3486, 3609
BbsI GAAGAC 3 cut(s) 150, 2800, 3674
Bbv12I GWGCWC 3 cut(s) 1050, 1332, 3578
BbvCI CCTCAGC 2 cut(s) 1250, 2553
BccI CCATC 3 cut(s) 86, 368, 2291
BceAI ACGGC 2 cut(s) 723, 3197
BciT130I CCWGG 2 cut(s) 2183, 3551
BciVI GTATCC 3 cut(s) 821, 1581, 3123
BclI TGATCA 3 cut(s) 1143, 1792, 3622
BcnI CCSGG 4 cut(s) 283, 1448, 2083, 3590
BcoDI GTCTC 3 cut(s) 467, 746, 966
BcuI ACTAGT 1 cut(s) 1177
BfaI CTAG 7 cut(s) 1178, 1695, 1913, 2123, 2255, 2997, 3284
BfmI CTRYAG 5 cut(s) 623, 1259, 3058, 3448, 3569
BfuAI ACCTGC 1 cut(s) 327
BfuI GTATCC 3 cut(s) 821, 1581, 3123
BglI GCCNNNNNGGC 2 cut(s) 600, 786
BlpI GCTNAGC 1 cut(s) 2787
Bme1390I CCNGG 6 cut(s) 283, 1448, 2083, 2183, 3551, 3590
Bme18I GGWCC 7 cut(s) 171, 241, 356, 572, 860, 1605, 2228
BmeRI GACNNNNNGTC 1 cut(s) 3265
BmeT110I CYCGRG 3 cut(s) 352, 796, 1331
BmgBI CACGTC 1 cut(s) 387
BmgT120I GGNCC 9 cut(s) 171, 241, 356, 572, 860, 1294, 1295, 1605, 2228
BmrFI CCNGG 6 cut(s) 283, 1448, 2083, 2183, 3551, 3590
BmrI ACTGGG 3 cut(s) 435, 896, 2456
BmsI GCATC 4 cut(s) 7, 61, 3043, 3437
BmuI ACTGGG 3 cut(s) 435, 896, 2456
BpiI GAAGAC 3 cut(s) 150, 2800, 3674
BpmI CTGGAG 6 cut(s) 629, 658, 737, 1386, 1735, 1817
Bpu10I CCTNAGC 3 cut(s) 1250, 2553, 2890
Bpu1102I GCTNAGC 1 cut(s) 2787
Bpu14I TTCGAA 2 cut(s) 1823, 3290
BpuEI CTTGAG 2 cut(s) 3192, 3407
BpuMI CCSGG 4 cut(s) 283, 1448, 2083, 3590
Bsa29I ATCGAT 1 cut(s) 1413
BsaBI GATNNNNATC 1 cut(s) 476
BsaHI GRCGYC 2 cut(s) 1005, 2853
BsaJI CCNNGG 3 cut(s) 390, 1609, 2082
BsaWI WCCGGW 3 cut(s) 1922, 1948, 3527
BsaXI ACNNNNNCTCC 2 cut(s) 2925, 2955
Bse118I RCCGGY 3 cut(s) 785, 2548, 3030
Bse1I ACTGG 9 cut(s) 430, 502, 612, 891, 1752, 1870, 2462, 2549, 2772
Bse3DI GCAATG 1 cut(s) 1731
Bse8I GATNNNNATC 1 cut(s) 476
BseAI TCCGGA 2 cut(s) 1922, 3527
BseBI CCWGG 2 cut(s) 2183, 3551
BseCI ATCGAT 1 cut(s) 1413
BseDI CCNNGG 3 cut(s) 390, 1609, 2082
BseGI GGATG 5 cut(s) 360, 432, 1008, 2478, 3249
BseJI GATNNNNATC 1 cut(s) 476
BseMI GCAATG 1 cut(s) 1731
BseNI ACTGG 9 cut(s) 430, 502, 612, 891, 1752, 1870, 2462, 2549, 2772
BseSI GKGCMC 2 cut(s) 1298, 3578
BseX3I CGGCCG 3 cut(s) 592, 736, 942
BseYI CCCAGC 2 cut(s) 1087, 1809
BsgI GTGCAG 1 cut(s) 3492
Bsh1236I CGCG 2 cut(s) 728, 3545
Bsh1285I CGRYCG 4 cut(s) 595, 739, 945, 3653
BshNI GGYRCC 1 cut(s) 953
BshVI ATCGAT 1 cut(s) 1413
BsiEI CGRYCG 4 cut(s) 595, 739, 945, 3653
BsiHKAI GWGCWC 3 cut(s) 1050, 1332, 3578
BsiHKCI CYCGRG 3 cut(s) 352, 796, 1331
BslFI GGGAC 8 cut(s) 342, 443, 1083, 1591, 1963, 2410, 2438, 3272
BsmAI GTCTC 3 cut(s) 467, 746, 966
BsmBI CGTCTC 1 cut(s) 746
BsmFI GGGAC 8 cut(s) 342, 443, 1083, 1591, 1963, 2410, 2438, 3272
BsmI GAATGC 1 cut(s) 1323
BsoBI CYCGRG 3 cut(s) 352, 796, 1331
Bsp119I TTCGAA 2 cut(s) 1823, 3290
Bsp120I GGGCCC 1 cut(s) 1294
Bsp1286I GDGCHC 7 cut(s) 258, 495, 1050, 1298, 1332, 2089, 3578
Bsp13I TCCGGA 2 cut(s) 1922, 3527
Bsp1407I TGTACA 1 cut(s) 1635
Bsp1720I GCTNAGC 1 cut(s) 2787
BspDI ATCGAT 1 cut(s) 1413
BspEI TCCGGA 2 cut(s) 1922, 3527
BspFNI CGCG 2 cut(s) 728, 3545
BspHI TCATGA 1 cut(s) 1795
BspMAI CTGCAG 4 cut(s) 627, 1263, 3062, 3573
BspMI ACCTGC 1 cut(s) 327
BspQI GCTCTTC 2 cut(s) 2094, 3155
BspT104I TTCGAA 2 cut(s) 1823, 3290
BspT107I GGYRCC 1 cut(s) 953
BsrDI GCAATG 1 cut(s) 1731
BsrFI RCCGGY 3 cut(s) 785, 2548, 3030
BsrGI TGTACA 1 cut(s) 1635
BsrI ACTGG 9 cut(s) 430, 502, 612, 891, 1752, 1870, 2462, 2549, 2772
BssAI RCCGGY 3 cut(s) 785, 2548, 3030
BssECI CCNNGG 3 cut(s) 390, 1609, 2082
BssNI GRCGYC 2 cut(s) 1005, 2853
BssSI CACGAG 2 cut(s) 3486, 3609
BssT1I CCWWGG 2 cut(s) 390, 1609
Bst2BI CACGAG 2 cut(s) 3486, 3609
Bst2UI CCWGG 2 cut(s) 2183, 3551
Bst4CI ACNGT 8 cut(s) 572, 995, 1394, 1538, 1574, 1977, 2813, 2941
Bst6I CTCTTC 5 cut(s) 122, 343, 2094, 3155, 3486
BstACI GRCGYC 2 cut(s) 1005, 2853
BstAPI GCANNNNNTGC 2 cut(s) 319, 1630
BstAUI TGTACA 1 cut(s) 1635
BstBI TTCGAA 2 cut(s) 1823, 3290
BstC8I GCNNGC 7 cut(s) 452, 969, 1403, 2261, 2531, 2550, 3239
BstEII GGTNACC 2 cut(s) 746, 2177
BstENI CCTNNNNNAGG 2 cut(s) 2046, 2995
BstF5I GGATG 5 cut(s) 360, 432, 1008, 2478, 3249
BstFNI CGCG 2 cut(s) 728, 3545
BstHHI GCGC 2 cut(s) 220, 730
BstMAI GTCTC 3 cut(s) 467, 746, 966
BstMCI CGRYCG 4 cut(s) 595, 739, 945, 3653
BstNI CCWGG 2 cut(s) 2183, 3551
BstNSI RCATGY 1 cut(s) 3638
BstPI GGTNACC 2 cut(s) 746, 2177
BstSCI CCNGG 6 cut(s) 281, 1446, 2081, 2181, 3549, 3588
BstSFI CTRYAG 5 cut(s) 623, 1259, 3058, 3448, 3569
BstSLI GKGCMC 2 cut(s) 1298, 3578
BstUI CGCG 2 cut(s) 728, 3545
BstV2I GAAGAC 3 cut(s) 150, 2800, 3674
BstX2I RGATCY 6 cut(s) 500, 1083, 1443, 2575, 3077, 3383
BstXI CCANNNNNNTGG 1 cut(s) 425
BstYI RGATCY 6 cut(s) 500, 1083, 1443, 2575, 3077, 3383
BstZI CGGCCG 3 cut(s) 592, 736, 942
Bsu15I ATCGAT 1 cut(s) 1413
BsuI GTATCC 3 cut(s) 821, 1581, 3123
BsuTUI ATCGAT 1 cut(s) 1413
BtrI CACGTC 1 cut(s) 387
BtsCI GGATG 5 cut(s) 360, 432, 1008, 2478, 3249
BtsI GCAGTG 5 cut(s) 1907, 2030, 2649, 3566, 3578
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 7 cut(s) 452, 969, 1403, 2261, 2531, 2550, 3239
CaiI CAGNNNCTG 1 cut(s) 760
CciI TCATGA 1 cut(s) 1795
CfoI GCGC 2 cut(s) 220, 730
Cfr10I RCCGGY 3 cut(s) 785, 2548, 3030
Cfr13I GGNCC 9 cut(s) 171, 241, 356, 572, 860, 1294, 1295, 1605, 2228
ClaI ATCGAT 1 cut(s) 1413
CseI GACGC 2 cut(s) 994, 2861
Csp6I GTAC 8 cut(s) 195, 1276, 1636, 2667, 2764, 3055, 3100, 3394
CspCI CAANNNNNGTGG 4 cut(s) 1866, 1901, 2353, 2388
CviQI GTAC 8 cut(s) 195, 1276, 1636, 2667, 2764, 3055, 3100, 3394
DrdI GACNNNNNNGTC 1 cut(s) 239
DriI GACNNNNNGTC 1 cut(s) 3265
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 5 cut(s) 592, 736, 787, 942, 2546
EagI CGGCCG 3 cut(s) 592, 736, 942
Eam1104I CTCTTC 5 cut(s) 122, 343, 2094, 3155, 3486
Eam1105I GACNNNNNGTC 1 cut(s) 3265
EarI CTCTTC 5 cut(s) 122, 343, 2094, 3155, 3486
EciI GGCGGA 1 cut(s) 1580
Ecl136II GAGCTC 2 cut(s) 1048, 1330
EclXI CGGCCG 3 cut(s) 592, 736, 942
Eco130I CCWWGG 2 cut(s) 390, 1609
Eco147I AGGCCT 1 cut(s) 3601
Eco24I GRGCYC 6 cut(s) 258, 495, 1050, 1298, 1332, 2089
Eco32I GATATC 3 cut(s) 291, 1242, 2830
Eco47I GGWCC 7 cut(s) 171, 241, 356, 572, 860, 1605, 2228
Eco52I CGGCCG 3 cut(s) 592, 736, 942
Eco53kI GAGCTC 2 cut(s) 1048, 1330
Eco57I CTGAAG 3 cut(s) 881, 2078, 2321
Eco88I CYCGRG 3 cut(s) 352, 796, 1331
Eco91I GGTNACC 2 cut(s) 746, 2177
EcoICRI GAGCTC 2 cut(s) 1048, 1330
EcoNI CCTNNNNNAGG 2 cut(s) 2046, 2995
EcoO109I RGGNCCY 3 cut(s) 356, 1295, 1605
EcoO65I GGTNACC 2 cut(s) 746, 2177
EcoRI GAATTC 1 cut(s) 2143
EcoRII CCWGG 2 cut(s) 2181, 3549
EcoRV GATATC 3 cut(s) 291, 1242, 2830
EcoT14I CCWWGG 2 cut(s) 390, 1609
EcoT22I ATGCAT 1 cut(s) 1789
EcoT38I GRGCYC 6 cut(s) 258, 495, 1050, 1298, 1332, 2089
ErhI CCWWGG 2 cut(s) 390, 1609
Esp3I CGTCTC 1 cut(s) 746
FalI AAGNNNNNCTT 4 cut(s) 1644, 1676, 2958, 2990
FaqI GGGAC 8 cut(s) 342, 443, 1083, 1591, 1963, 2410, 2438, 3272
FauI CCCGC 5 cut(s) 517, 733, 1430, 1528, 1577
FbaI TGATCA 3 cut(s) 1143, 1792, 3622
FblI GTMKAC 1 cut(s) 3447
FokI GGATG 5 cut(s) 347, 419, 995, 2485, 3236
FriOI GRGCYC 6 cut(s) 258, 495, 1050, 1298, 1332, 2089
FspBI CTAG 7 cut(s) 1178, 1695, 1913, 2123, 2255, 2997, 3284
GlaI GCGC 2 cut(s) 219, 729
GsaI CCCAGC 2 cut(s) 1091, 1813
GsuI CTGGAG 6 cut(s) 629, 658, 737, 1386, 1735, 1817
HgaI GACGC 2 cut(s) 994, 2861
HhaI GCGC 2 cut(s) 220, 730
Hin1I GRCGYC 2 cut(s) 1005, 2853
Hin6I GCGC 2 cut(s) 218, 728
HinP1I GCGC 2 cut(s) 218, 728
HincII GTYRAC 2 cut(s) 803, 821
HindII GTYRAC 2 cut(s) 803, 821
HindIII AAGCTT 2 cut(s) 2489, 2730
HphI GGTGA 6 cut(s) 136, 775, 1523, 2071, 2312, 3052
Hpy166II GTNNAC 7 cut(s) 803, 812, 821, 919, 1863, 3448, 3576
Hpy8I GTNNAC 7 cut(s) 803, 812, 821, 919, 1863, 3448, 3576
HpyAV CCTTC 8 cut(s) 635, 851, 1646, 1815, 2012, 2466, 3001, 3612
HpyCH4III ACNGT 8 cut(s) 572, 995, 1394, 1538, 1574, 1977, 2813, 2941
HpyCH4IV ACGT 1 cut(s) 386
HpySE526I ACGT 1 cut(s) 386
Hsp92I GRCGYC 2 cut(s) 1005, 2853
HspAI GCGC 2 cut(s) 218, 728
KflI GGGWCCC 2 cut(s) 356, 1605
Kpn2I TCCGGA 2 cut(s) 1922, 3527
KroI GCCGGC 1 cut(s) 2548
KroNI GCCGGC 1 cut(s) 2550
Ksp22I TGATCA 3 cut(s) 1143, 1792, 3622
LguI GCTCTTC 2 cut(s) 2094, 3155
LweI GCATC 4 cut(s) 7, 61, 3043, 3437
MaeI CTAG 7 cut(s) 1178, 1695, 1913, 2123, 2255, 2997, 3284
MaeII ACGT 1 cut(s) 386
MfeI CAATTG 3 cut(s) 135, 2107, 2679
MflI RGATCY 6 cut(s) 500, 1083, 1443, 2575, 3077, 3383
MhlI GDGCHC 7 cut(s) 258, 495, 1050, 1298, 1332, 2089, 3578
MlyI GAGTC 8 cut(s) 218, 469, 591, 808, 826, 910, 2392, 3359
MmeI TCCRAC 3 cut(s) 1053, 2654, 3178
Mph1103I ATGCAT 1 cut(s) 1789
MroI TCCGGA 2 cut(s) 1922, 3527
MroNI GCCGGC 1 cut(s) 2548
MroXI GAANNNNTTC 1 cut(s) 2733
MslI CAYNNNNRTG 1 cut(s) 3571
MspA1I CMGCKG 3 cut(s) 695, 967, 1437
MspR9I CCNGG 6 cut(s) 283, 1448, 2083, 2183, 3551, 3590
MunI CAATTG 3 cut(s) 135, 2107, 2679
Mva1269I GAATGC 1 cut(s) 1323
MvaI CCWGG 2 cut(s) 2183, 3551
MvnI CGCG 2 cut(s) 728, 3545
NaeI GCCGGC 1 cut(s) 2550
NciI CCSGG 4 cut(s) 283, 1448, 2083, 3590
NgoMIV GCCGGC 1 cut(s) 2548
NmeAIII GCCGAG 4 cut(s) 570, 579, 714, 1329
NmuCI GTSAC 8 cut(s) 208, 273, 538, 1263, 1538, 1774, 2300, 3606
NsiI ATGCAT 1 cut(s) 1789
NspI RCATGY 1 cut(s) 3638
NspV TTCGAA 2 cut(s) 1823, 3290
OliI CACNNNNGTG 1 cut(s) 3571
PaeR7I CTCGAG 1 cut(s) 1331
PagI TCATGA 1 cut(s) 1795
PceI AGGCCT 1 cut(s) 3601
PciI ACATGT 1 cut(s) 3634
PciSI GCTCTTC 2 cut(s) 2094, 3155
PcsI WCGNNNNNNNCGW 4 cut(s) 1232, 1763, 2743, 3345
PctI GAATGC 1 cut(s) 1323
PdiI GCCGGC 1 cut(s) 2550
PdmI GAANNNNTTC 1 cut(s) 2733
PflFI GACNNNGTC 1 cut(s) 2401
PflMI CCANNNNNTGG 1 cut(s) 2468
PleI GAGTC 8 cut(s) 218, 468, 591, 807, 825, 909, 2392, 3358
PpsI GAGTC 8 cut(s) 218, 468, 591, 807, 825, 909, 2392, 3358
PpuMI RGGWCCY 2 cut(s) 356, 1605
PscI ACATGT 1 cut(s) 3634
Psp124BI GAGCTC 2 cut(s) 1050, 1332
Psp5II RGGWCCY 2 cut(s) 356, 1605
Psp6I CCWGG 2 cut(s) 2181, 3549
PspEI GGTNACC 2 cut(s) 746, 2177
PspFI CCCAGC 2 cut(s) 1087, 1809
PspGI CCWGG 2 cut(s) 2181, 3549
PspOMI GGGCCC 1 cut(s) 1294
PspPI GGNCC 9 cut(s) 171, 241, 356, 572, 860, 1294, 1295, 1605, 2228
PspPPI RGGWCCY 2 cut(s) 356, 1605
PsrI GAACNNNNNNTAC 4 cut(s) 1738, 1770, 2747, 2779
PstI CTGCAG 4 cut(s) 627, 1263, 3062, 3573
PstNI CAGNNNCTG 1 cut(s) 760
PsuI RGATCY 6 cut(s) 500, 1083, 1443, 2575, 3077, 3383
PsyI GACNNNGTC 1 cut(s) 2401
PvuII CAGCTG 2 cut(s) 695, 967
RsaI GTAC 8 cut(s) 196, 1277, 1637, 2668, 2765, 3056, 3101, 3395
RsaNI GTAC 8 cut(s) 195, 1276, 1636, 2667, 2764, 3055, 3100, 3394
RseI CAYNNNNRTG 1 cut(s) 3571
SacI GAGCTC 2 cut(s) 1050, 1332
SapI GCTCTTC 2 cut(s) 2094, 3155
Sau96I GGNCC 9 cut(s) 171, 241, 356, 572, 860, 1294, 1295, 1605, 2228
SchI GAGTC 8 cut(s) 218, 469, 591, 808, 826, 910, 2392, 3359
ScrFI CCNGG 6 cut(s) 283, 1448, 2083, 2183, 3551, 3590
SduI GDGCHC 7 cut(s) 258, 495, 1050, 1298, 1332, 2089, 3578
SfaNI GCATC 4 cut(s) 7, 61, 3043, 3437
SfcI CTRYAG 5 cut(s) 623, 1259, 3058, 3448, 3569
SfiI GGCCNNNNNGGCC 1 cut(s) 600
Sfr274I CTCGAG 1 cut(s) 1331
SfuI TTCGAA 2 cut(s) 1823, 3290
SinI GGWCC 7 cut(s) 171, 241, 356, 572, 860, 1605, 2228
SlaI CTCGAG 1 cut(s) 1331
SmiMI CAYNNNNRTG 1 cut(s) 3571
SmlI CTYRAG 3 cut(s) 1331, 3207, 3422
SmoI CTYRAG 3 cut(s) 1331, 3207, 3422
SpeI ACTAGT 1 cut(s) 1177
SseBI AGGCCT 1 cut(s) 3601
SspI AATATT 1 cut(s) 3277
SspMI CTAG 7 cut(s) 1178, 1695, 1913, 2123, 2255, 2997, 3284
SstI GAGCTC 2 cut(s) 1050, 1332
StuI AGGCCT 1 cut(s) 3601
StyD4I CCNGG 6 cut(s) 281, 1446, 2081, 2181, 3549, 3588
StyI CCWWGG 2 cut(s) 390, 1609
TaaI ACNGT 8 cut(s) 572, 995, 1394, 1538, 1574, 1977, 2813, 2941
TaiI ACGT 1 cut(s) 389
TaqII GACCGA 2 cut(s) 229, 3639
TatI WGTACW 5 cut(s) 194, 1635, 2666, 2763, 3099
TseFI GTSAC 8 cut(s) 208, 273, 538, 1263, 1538, 1774, 2300, 3606
Tsp45I GTSAC 8 cut(s) 208, 273, 538, 1263, 1538, 1774, 2300, 3606
TspDTI ATGAA 7 cut(s) 48, 848, 1130, 1475, 1618, 1950, 2768
TspGWI ACGGA 4 cut(s) 729, 904, 1224, 3126
Tth111I GACNNNGTC 1 cut(s) 2401
Van91I CCANNNNNTGG 1 cut(s) 2468
VneI GTGCAC 1 cut(s) 3574
VpaK11BI GGWCC 7 cut(s) 171, 241, 356, 572, 860, 1605, 2228
XagI CCTNNNNNAGG 2 cut(s) 2046, 2995
XapI RAATTY 9 cut(s) 187, 947, 1687, 1702, 1819, 1927, 2143, 2876, 3673
XceI RCATGY 1 cut(s) 3638
XcmI CCANNNNNNNNNTGG 3 cut(s) 883, 964, 2832
XhoI CTCGAG 1 cut(s) 1331
XmiI GTMKAC 1 cut(s) 3447
XmnI GAANNNNTTC 1 cut(s) 2733
XspI CTAG 7 cut(s) 1178, 1695, 1913, 2123, 2255, 2997, 3284
Zsp2I ATGCAT 1 cut(s) 1789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.