RLG00000032624
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014415
Physical Location & Seq
Forward (+)
17071793 .. 17076286
4494 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032624

Sequence Viewer

Length: 3777 bp
ATGGCTGCAACAAGAACAAAGATGTCAATTTTACCACTTTTTCTTATTCTTCTTGTCATTTCTTCTATTTTTGTGGAGGCAGAGCTTGATAGCAATACGACTCTAAAAGTGCTTTTGGAGGTGAAGAAGTCGTTTTTGGAGGACCCAATATCCGAAACAGCCTTGCATGGATGGTCTGAAAGCAACCCAAATTTCTGTACTTGGAGAGGACTGACATGCGGGTCTGACTCGGTCGATGACTCGGTCCAAGTGGTCGGACTCAACCTTTCCGACTCGTCACTCCGCGGGTCAATATCTCCCCAACTCGGTAGTTTGAAGTACCTGCTCCACCTTGATCTCTCTTCTAACGGCCTCACGGGTCCCATCCCACCAGCCCTCTCAAACCTCTCTTCCTTGGAATCTTTGCTCCTCTTTACGAACCGACTCGGTGGACCCATCCCGAGTGAACTCGGCTTGCTCACGAGTCTCCGAGTCATGAGAATCGGTGACAATGAGCTAACCGGGTCCATTCCAGCCTCATTTGGTAACCTTGTCAATTTGGTCACCCTTGCGGTGGCATCATGTGGTCTCAACGGTCCAATACCACCCCAACTCGGCCGGCTCGGTCAGCTGGAGAATTTAATCCTCCAGCAGAACCAACTCGAGGGTCCAATCCCCGCCGAGTTGGGGAACTGTTCTAGTCTCACTGTGTTCACGGCTGCTGAAAATAAACTCAACGGCTCGATTCCCCGGGAATTGGGTCAGCTCAACAATCTCCAGCTACTCAATTTGGTCAACAACAGCCTCTCCGGGGAGATACCGAGTCAACTCGGTGGGATGAGTCAACTCGGGTACCTGAACTTTATGGGGAACCAACTTGAGGGTGCAATACCCAAGTCCTTGGCTCAATTGGGCAATCTTCAGAATCTTGATTTGTCCATTAACAAGCTCACAGGAGGAATCCCAGAGGAACTAGGTAACATGGGTCAACTGGTTTACTTGGTTTTGTCCAACAACAATCTCTCTGGTGTTATACCAAAGACTATATGCTCCAATGCAACTAGTTTGGAGCACTTGGTGATATCCGATTCTCAAATTTTCGGCGAAATCCCAGCTGAGCTGAGCCAGTGTCAATCCCTGAAGCAACTTGACTTGTCCAACAATTCAATCACTGGATCAATTCCACTCGAACTGTATGGCTTGGTGGGGTTAACTGACCTCTTGCTCCACAACAACAGCTTGGTTGGTTCAATTTCTCCTTATATAGGCAACCTCAGCAACCTCCAGACTCTTGTTTTGTATCACAACAATTTGCAGGGTCCCCTGCCGAAAGAGATTGGTATGCTGGGGCAGCTAGAGATTTTGTATCTCTATGATAACCGGCTAAGTGGTGAAATCCCAATTGAGATAGGGAATTGTTCGAGTTTGCAGATGATTGACTTCTTTGGGAACAAGTTCAGTGGGGAGATTCCAATCACTATTGGGAGGCTCAAAGATTTGAACTTTCTTCATATCAGACAGAATGAGCTAGTGGGTGAGCTTCCTGCCACATTGGGCAACTGTCGCCAACTAACTATCCTGGACTTGGCGGATAACCGTTTGTCTGGTGGCATTCCTATTACATTTGGGTCTCTTCAAGCAATGCAGCAGTTCATGCTATACAACAATTCACTTGAAGGTAATCTTCCTGAAACATTGGTTGATATGGCGAATTTGACTAGAGTGAATTTGTCGAAAAACAAATTGAATGGAAGCATTGCTGCATTATGTAGCTCGAATTCATTCCTTTCTTTTGACGTCACTGACAATGCCTTTGATGGTGAGATTCCTCCCCATCTGGGAAATTCAACCACCCTTGAGAGGTTGCGATTGGGGAACAACCAATTTACGGGTCCAATCCCAGTTACATTGGGAAAAATCAGTGAGCTATCACTGTTAGATGTGTCAGGGAATTCACTCACTGGACCAATCCCAGCTGAGCTTTCATCGTGCAAGAAATTGTCTCATTTTGATTTGAATGACAACCTTCTTTCTGGCATCATTCCCATGTGGCTTGGAAGTTTGCCTCAGCTAGGAGAGTTGAAGCTCTCCTCAAATCGATTCACAGGGCCACTTCCCCAAGAGTTGTTCAATTGTTCTAAACTGCTAGTGCTTTCTTTGAATGACAATTTGCTCAATGGAACCCTCCCTGCTGAGATTGGCAACCTGGGTTCTCTCAATGTCCTAAACCTCGACCACAACCAATTCTCGGGACCAATGCCCCCAGCCATTGGTAAGCTAGGCAACCTCTATGAGCTTAGGCTCTCCCAAAACAGCTTTGATGGTGACATACCCTATGAGCTTGGACAACTCCAAAATCTTCAAAGCATGTTAGACCTCAGTTCCAATAAACTTAATGGTCAGATCCCGGCATCTATTGGGACACTATCAAAACTTGAGGCGCTTAATCTATCTCACAATCAACTTAGTGGAGAAGTCCCCTCTACAATTGGTGCGATGAGTAGCTTGGGAAAGCTTAATCTCACTTACAACAATCTGGAAGGAAAATTGAGCAAGCAACTCTCACACTGGCCAGCCGAGGCCTTTGCAGGAAATTCACATCTTTGTGGGAACCCTCTTGGTAGCTGCAATGGCAGCAAGAACCAGTCAGGCCCAAATGAATTGACTGTGATTGTCATTTCTGCATTATGTACTTTAGCTGCAATTGCCCTGCTAATATTTGGGGCTGCTTCTTTCTTGAAACACAGGCGAGAAGCACTTAGAAAAGCCAGTGAAGTGAACTGCGTGTACTCATCGAGTTCTTCTCATGCTAAGCGGAGACTGCTATTTTCAAATGGTTCTGTCAAGCCAGATTTCAAGTGGAAAGACATCATGGATTCCACCAACAATCTAAGTAAAGAGTTTATGATCGGATCAGGAGGATCCGGAGTAGTCTACAGAGCTGAATTGTCTACCGGAGAAATTGTGGCAGTGAAGAAGATGTTGTACAAGGATGACCTTATGTCAAATAAAAGCTTCACAAGGGAGATTAAGACGCTTGGGAGGATAAGGCACAGGCATTTAGTGAAGCTAATGGGGCATTGTAGCAACAAAGGAGCAGGCTCCAATCTGTTGATCTATGAGTACATGGAAAACGGAAGCGTATGGGATTGGATACACAAAGAACAAGTTAACAGCAAGAAGAAGAGCCTTGATTGGGAAGCAAGGTTAAAGATTGCAGTTGGGTTAGCTCAAGGAGTGGAGTATCTCCACCATGACTGTGTGCCAAAGATCATTCACAGGGACATTAAATCAAGCAATGTCTTGCTAGACTCAAATATGGAGGCACATCTTGGGGATTTTGGCCTTGCCAAGACACTCAATGAGAATTTTGAGTCCAACACAGAATCAACTACATGGTTTGCTGGATCCTACGGTTACATAGCCCCAGAGTATGCATATTCGCTCAAGGCGACAGAAAAGAGTGATGTCTACAGCATGGGAATTGTGCTAATGGAGCTCGTCAGCGGAAAAATGCCAACGGATGCATCTTTTGGAGAGGAGATGGACATGGTGAGATGGGTGGAGACGCATATTGAAATGCAAGATCAAATTCGTGAGGAGTTGATAGACCCTGCACTGAGACCACTCTTATCCGGGGAAGAATATGCAGCATTCCAAGTACTAGAGATAGCACTGCAGTGCACAAAAACCAGCCCGGCAGAGAGGCCTTCATCACGGCAAGCCTGTGATCAGTTATTACATGTTTTCAATCATAGGATGGTGGAGTTTGATAAGATGAATGTAGATAGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1259

Amino Acids

136.66

Weight (kDa)

5.34

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 73 4.1e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 149 - 258 6.5e-08 Leucine-rich repeat region
LRR_14 PF23598 268 - 378 1.3e-07 Leucine-rich repeat region
LRR_8 PF13855 348 - 407 2.1e-06 Leucine rich repeat
LRR_14 PF23598 365 - 474 2.4e-08 Leucine-rich repeat region
LRR_14 PF23598 481 - 571 9.4e-07 Leucine-rich repeat region
LRR_8 PF13855 515 - 575 1.3e-07 Leucine rich repeat
LRR_14 PF23598 706 - 782 1.6e-06 Leucine-rich repeat region
LRR_14 PF23598 759 - 837 1e-06 Leucine-rich repeat region
LRR_8 PF13855 783 - 839 4.5e-06 Leucine rich repeat
Pkinase PF00069 962 - 1238 2.5e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 962 - 1236 1.2e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014415)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20140
fragaria_vesca FvH4_3g13270 FvH4_3g13270 FvH4_3g13270
malus_domestica MD05G1243600.v1.1 MD10G1225500.v1.1
prunus_persica Prupe.4G120100_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0021741
rosa_laevigata RLG00000032624
rosa_multiflora Rmu_ssc0000233.1_g000008
rosa_roxburghii Rroxscaffold_1G00056980
rosa_rugosa Rorug05G0068900
rosa_samantha Rh5AG157400 Rh5BG157100 Rh5CG171200 Rh5DG157300
rosa_wichuraiana Rw5G014030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 220
AatII GACGTC 1 cut(s) 1779
Acc36I ACCTGC 1 cut(s) 330
Acc65I GGTACC 1 cut(s) 831
AccB1I GGYRCC 1 cut(s) 831
AccB7I CCANNNNNTGG 1 cut(s) 2249
AccI GTMKAC 3 cut(s) 2913, 2930, 3450
AccII CGCG 1 cut(s) 285
AccIII TCCGGA 1 cut(s) 2903
AciI CCGC 8 cut(s) 219, 283, 285, 551, 657, 1568, 2794, 3486
AclWI GGATC 7 cut(s) 1162, 2377, 2895, 2899, 2908, 3381, 3394
AcoI YGGCCR 2 cut(s) 595, 2549
AcuI CTGAAG 2 cut(s) 884, 1139
AcyI GRCGYC 1 cut(s) 1776
AdeI CACNNNGTG 1 cut(s) 1057
AfaI GTAC 8 cut(s) 199, 320, 833, 2671, 2768, 2966, 3104, 3642
AflIII ACRYGT 1 cut(s) 3721
AhdI GACNNNNNGTC 1 cut(s) 2980
AhlI ACTAGT 1 cut(s) 1040
AjnI CCWGG 2 cut(s) 1557, 2184
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
AleI CACNNNNGTG 1 cut(s) 3658
Alw21I GWGCWC 3 cut(s) 1053, 3480, 3665
Alw26I GTCTC 8 cut(s) 470, 572, 686, 1614, 1986, 2791, 3539, 3595
Alw44I GTGCAC 1 cut(s) 3661
AlwI GGATC 7 cut(s) 1162, 2377, 2895, 2899, 2908, 3381, 3394
Ama87I CYCGRG 5 cut(s) 439, 641, 729, 827, 2227
Aor13HI TCCGGA 1 cut(s) 2903
AoxI GGCC 8 cut(s) 349, 595, 2087, 2549, 2559, 2629, 3322, 3686
ApaLI GTGCAC 1 cut(s) 3661
ArsI GACNNNNNNTTYG 4 cut(s) 1258, 1290, 1775, 1807
Asp700I GAANNNNTTC 2 cut(s) 1433, 1760
Asp718I GGTACC 1 cut(s) 831
AspLEI GCGC 1 cut(s) 2422
AsuC2I CCSGG 7 cut(s) 502, 730, 731, 790, 2387, 3616, 3677
AsuHPI GGTGA 9 cut(s) 133, 497, 535, 1069, 1382, 1526, 1811, 2315, 3544
AvaI CYCGRG 5 cut(s) 439, 641, 729, 827, 2227
BaeGI GKGCMC 1 cut(s) 3665
BaeI ACNNNNGTAYC 2 cut(s) 815, 848
BalI TGGCCA 1 cut(s) 2551
BamHI GGATCC 2 cut(s) 2900, 3386
BanI GGYRCC 1 cut(s) 831
BanII GRGCYC 1 cut(s) 3480
BarI GAAGNNNNNNTAC 2 cut(s) 2948, 2980
BauI CACGAG 1 cut(s) 460
Bbv12I GWGCWC 3 cut(s) 1053, 3480, 3665
BbvCI CCTCAGC 2 cut(s) 1253, 2046
BbvI GCAGC 9 cut(s) 685, 1342, 1636, 1726, 2591, 2625, 2665, 2692, 3641
BccI CCATC 9 cut(s) 165, 371, 443, 1790, 1821, 2294, 3517, 3531, 3733
BceAI ACGGC 4 cut(s) 364, 711, 733, 3713
BcgI CGANNNNNNTGC 2 cut(s) 2546, 2580
BciT130I CCWGG 2 cut(s) 1559, 2186
BciVI GTATCC 1 cut(s) 3126
BclI TGATCA 1 cut(s) 3709
BcnI CCSGG 7 cut(s) 502, 730, 731, 790, 2387, 3616, 3677
BcoDI GTCTC 8 cut(s) 470, 572, 686, 1614, 1986, 2791, 3539, 3595
BcuI ACTAGT 1 cut(s) 1040
BfmI CTRYAG 3 cut(s) 2914, 3451, 3656
BfoI RGCGCY 1 cut(s) 2423
BfuAI ACCTGC 1 cut(s) 330
BfuI GTATCC 1 cut(s) 3126
BlpI GCTNAGC 4 cut(s) 1095, 1100, 1956, 2790
BmcAI AGTACT 1 cut(s) 3642
Bme1390I CCNGG 9 cut(s) 502, 730, 731, 790, 1559, 2186, 2387, 3616, 3677
BmeRI GACNNNNNGTC 1 cut(s) 2980
BmeT110I CYCGRG 5 cut(s) 439, 641, 729, 827, 2227
BmrFI CCNGG 9 cut(s) 502, 730, 731, 790, 1559, 2186, 2387, 3616, 3677
BmrI ACTGGG 1 cut(s) 1874
BmsI GCATC 5 cut(s) 566, 2025, 2399, 3493, 3515
BmuI ACTGGG 1 cut(s) 1874
BplI GAGNNNNNCTC 2 cut(s) 2767, 2799
BpmI CTGGAG 4 cut(s) 611, 632, 740, 1247
Bpu10I CCTNAGC 3 cut(s) 1253, 2046, 2276
Bpu1102I GCTNAGC 4 cut(s) 1095, 1100, 1956, 2790
BpuEI CTTGAG 5 cut(s) 878, 1856, 2435, 3195, 3410
BpuMI CCSGG 7 cut(s) 502, 730, 731, 790, 2387, 3616, 3677
Bsa29I ATCGAT 1 cut(s) 2077
BsaBI GATNNNNATC 1 cut(s) 1451
BsaHI GRCGYC 1 cut(s) 1776
BsaI GGTCTC 3 cut(s) 572, 1614, 3595
BsaJI CCNNGG 9 cut(s) 283, 393, 728, 729, 789, 879, 2185, 2556, 3615
BsaWI WCCGGW 2 cut(s) 2903, 2933
BsaXI ACNNNNNCTCC 4 cut(s) 770, 800, 2928, 2958
Bse118I RCCGGY 2 cut(s) 597, 1359
Bse1I ACTGG 8 cut(s) 975, 1105, 1156, 1880, 1945, 2552, 2623, 2748
Bse3DI GCAATG 4 cut(s) 1626, 1734, 2614, 3283
Bse8I GATNNNNATC 1 cut(s) 1451
BseAI TCCGGA 1 cut(s) 2903
BseBI CCWGG 2 cut(s) 1559, 2186
BseCI ATCGAT 1 cut(s) 2077
BseDI CCNNGG 9 cut(s) 283, 393, 728, 729, 789, 879, 2185, 2556, 3615
BseGI GGATG 7 cut(s) 176, 363, 435, 822, 2977, 3508, 3744
BseJI GATNNNNATC 1 cut(s) 1451
BseMI GCAATG 4 cut(s) 1626, 1734, 2614, 3283
BseMII CTCAG 8 cut(s) 1086, 1091, 1267, 1947, 2060, 2163, 2371, 3590
BseNI ACTGG 8 cut(s) 975, 1105, 1156, 1880, 1945, 2552, 2623, 2748
BseRI GAGGAG 4 cut(s) 398, 2059, 3533, 3593
BseSI GKGCMC 1 cut(s) 3665
BseX3I CGGCCG 1 cut(s) 595
BseXI GCAGC 9 cut(s) 685, 1342, 1636, 1726, 2591, 2625, 2665, 2692, 3641
BseYI CCCAGC 4 cut(s) 1090, 1324, 1951, 2242
BsgI GTGCAG 1 cut(s) 3579
Bsh1236I CGCG 1 cut(s) 285
Bsh1285I CGRYCG 2 cut(s) 234, 598
BshFI GGCC 8 cut(s) 351, 597, 2089, 2551, 2561, 2631, 3324, 3688
BshNI GGYRCC 1 cut(s) 831
BshVI ATCGAT 1 cut(s) 2077
BsiEI CGRYCG 2 cut(s) 234, 598
BsiHKAI GWGCWC 3 cut(s) 1053, 3480, 3665
BsiHKCI CYCGRG 5 cut(s) 439, 641, 729, 827, 2227
BslFI GGGAC 6 cut(s) 345, 1284, 2244, 2413, 2441, 3275
BsmAI GTCTC 8 cut(s) 470, 572, 686, 1614, 1986, 2791, 3539, 3595
BsmBI CGTCTC 1 cut(s) 3539
BsmFI GGGAC 6 cut(s) 345, 1284, 2244, 2413, 2441, 3275
BsmI GAATGC 2 cut(s) 1590, 3632
BsnI GGCC 8 cut(s) 351, 597, 2089, 2551, 2561, 2631, 3324, 3688
Bso31I GGTCTC 3 cut(s) 572, 1614, 3595
BsoBI CYCGRG 5 cut(s) 439, 641, 729, 827, 2227
Bsp1286I GDGCHC 3 cut(s) 1053, 3480, 3665
Bsp13I TCCGGA 1 cut(s) 2903
Bsp1407I TGTACA 1 cut(s) 2964
Bsp1720I GCTNAGC 4 cut(s) 1095, 1100, 1956, 2790
BspACI CCGC 8 cut(s) 219, 283, 285, 551, 657, 1568, 2794, 3486
BspANI GGCC 8 cut(s) 351, 597, 2089, 2551, 2561, 2631, 3324, 3688
BspCNI CTCAG 8 cut(s) 1087, 1092, 1266, 1948, 2059, 2164, 2370, 3591
BspDI ATCGAT 1 cut(s) 2077
BspEI TCCGGA 1 cut(s) 2903
BspFNI CGCG 1 cut(s) 285
BspHI TCATGA 1 cut(s) 474
BspMAI CTGCAG 1 cut(s) 3660
BspMI ACCTGC 1 cut(s) 330
BspPI GGATC 7 cut(s) 1162, 2377, 2895, 2899, 2908, 3381, 3394
BspQI GCTCTTC 1 cut(s) 3158
BspT107I GGYRCC 1 cut(s) 831
BspTNI GGTCTC 3 cut(s) 572, 1614, 3595
BsrDI GCAATG 4 cut(s) 1626, 1734, 2614, 3283
BsrFI RCCGGY 2 cut(s) 597, 1359
BsrGI TGTACA 1 cut(s) 2964
BsrI ACTGG 8 cut(s) 975, 1105, 1156, 1880, 1945, 2552, 2623, 2748
BssAI RCCGGY 2 cut(s) 597, 1359
BssECI CCNNGG 9 cut(s) 283, 393, 728, 729, 789, 879, 2185, 2556, 3615
BssNI GRCGYC 1 cut(s) 1776
BssSI CACGAG 1 cut(s) 460
BssT1I CCWWGG 2 cut(s) 393, 879
Bst2BI CACGAG 1 cut(s) 460
Bst2UI CCWGG 2 cut(s) 1559, 2186
Bst6I CTCTTC 4 cut(s) 346, 394, 1617, 3158
BstACI GRCGYC 1 cut(s) 1776
BstAPI GCANNNNNTGC 1 cut(s) 1633
BstAUI TGTACA 1 cut(s) 2964
BstC8I GCNNGC 6 cut(s) 455, 599, 2534, 2553, 3079, 3702
BstDSI CCRYGG 1 cut(s) 283
BstEII GGTNACC 2 cut(s) 524, 541
BstENI CCTNNNNNAGG 2 cut(s) 1242, 2049
BstF5I GGATG 7 cut(s) 176, 363, 435, 822, 2977, 3508, 3744
BstFNI CGCG 1 cut(s) 285
BstH2I RGCGCY 1 cut(s) 2423
BstHHI GCGC 1 cut(s) 2422
BstMAI GTCTC 8 cut(s) 470, 572, 686, 1614, 1986, 2791, 3539, 3595
BstMCI CGRYCG 2 cut(s) 234, 598
BstNI CCWGG 2 cut(s) 1559, 2186
BstNSI RCATGY 3 cut(s) 219, 2350, 3725
BstPI GGTNACC 2 cut(s) 524, 541
BstSCI CCNGG 9 cut(s) 500, 728, 729, 788, 1557, 2184, 2385, 3614, 3675
BstSFI CTRYAG 3 cut(s) 2914, 3451, 3656
BstSLI GKGCMC 1 cut(s) 3665
BstUI CGCG 1 cut(s) 285
BstV1I GCAGC 9 cut(s) 685, 1342, 1636, 1726, 2591, 2625, 2665, 2692, 3641
BstX2I RGATCY 3 cut(s) 2382, 2900, 3386
BstXI CCANNNNNNTGG 1 cut(s) 880
BstYI RGATCY 3 cut(s) 2382, 2900, 3386
BstZI CGGCCG 1 cut(s) 595
Bsu15I ATCGAT 1 cut(s) 2077
BsuI GTATCC 1 cut(s) 3126
BsuRI GGCC 8 cut(s) 351, 597, 2089, 2551, 2561, 2631, 3324, 3688
BsuTUI ATCGAT 1 cut(s) 2077
BtgI CCRYGG 1 cut(s) 283
BtgZI GCGATG 1 cut(s) 2489
BtsCI GGATG 7 cut(s) 176, 363, 435, 822, 2977, 3508, 3744
BtsI GCAGTG 3 cut(s) 2955, 3653, 3665
BveI ACCTGC 1 cut(s) 330
Cac8I GCNNGC 6 cut(s) 455, 599, 2534, 2553, 3079, 3702
CciI TCATGA 1 cut(s) 474
CfoI GCGC 1 cut(s) 2422
Cfr10I RCCGGY 2 cut(s) 597, 1359
Cfr42I CCGCGG 1 cut(s) 286
Cfr9I CCCGGG 1 cut(s) 729
ClaI ATCGAT 1 cut(s) 2077
CseI GACGC 2 cut(s) 3022, 3556
Csp6I GTAC 8 cut(s) 198, 319, 832, 2670, 2767, 2965, 3103, 3641
CspCI CAANNNNNGTGG 2 cut(s) 1420, 1455
CviQI GTAC 8 cut(s) 198, 319, 832, 2670, 2767, 2965, 3103, 3641
DraIII CACNNNGTG 1 cut(s) 1057
DrdI GACNNNNNNGTC 1 cut(s) 220
DriI GACNNNNNGTC 1 cut(s) 2980
DseDI GACNNNNNNGTC 1 cut(s) 220
EaeI YGGCCR 2 cut(s) 595, 2549
EagI CGGCCG 1 cut(s) 595
Eam1104I CTCTTC 4 cut(s) 346, 394, 1617, 3158
Eam1105I GACNNNNNGTC 1 cut(s) 2980
EarI CTCTTC 4 cut(s) 346, 394, 1617, 3158
EciI GGCGGA 1 cut(s) 1583
Ecl136II GAGCTC 1 cut(s) 3478
EclXI CGGCCG 1 cut(s) 595
Eco130I CCWWGG 2 cut(s) 393, 879
Eco147I AGGCCT 2 cut(s) 2561, 3688
Eco24I GRGCYC 1 cut(s) 3480
Eco31I GGTCTC 3 cut(s) 572, 1614, 3595
Eco32I GATATC 1 cut(s) 1062
Eco52I CGGCCG 1 cut(s) 595
Eco53kI GAGCTC 1 cut(s) 3478
Eco57I CTGAAG 2 cut(s) 884, 1139
Eco88I CYCGRG 5 cut(s) 439, 641, 729, 827, 2227
Eco91I GGTNACC 2 cut(s) 524, 541
EcoICRI GAGCTC 1 cut(s) 3478
EcoNI CCTNNNNNAGG 2 cut(s) 1242, 2049
EcoO109I RGGNCCY 3 cut(s) 142, 359, 1298
EcoO65I GGTNACC 2 cut(s) 524, 541
EcoRI GAATTC 2 cut(s) 1756, 1930
EcoRII CCWGG 2 cut(s) 1557, 2184
EcoRV GATATC 1 cut(s) 1062
EcoT14I CCWWGG 2 cut(s) 393, 879
EcoT22I ATGCAT 2 cut(s) 3418, 3508
EcoT38I GRGCYC 1 cut(s) 3480
ErhI CCWWGG 2 cut(s) 393, 879
Esp3I CGTCTC 1 cut(s) 3539
FalI AAGNNNNNCTT 2 cut(s) 1647, 1679
FaqI GGGAC 6 cut(s) 345, 1284, 2244, 2413, 2441, 3275
FauI CCCGC 3 cut(s) 212, 278, 664
FbaI TGATCA 1 cut(s) 3709
FblI GTMKAC 3 cut(s) 2913, 2930, 3450
FokI GGATG 7 cut(s) 183, 350, 422, 829, 2984, 3515, 3751
FriOI GRGCYC 1 cut(s) 3480
GlaI GCGC 1 cut(s) 2421
GsaI CCCAGC 4 cut(s) 1094, 1328, 1955, 2246
GsuI CTGGAG 4 cut(s) 611, 632, 740, 1247
HaeII RGCGCY 1 cut(s) 2423
HaeIII GGCC 8 cut(s) 351, 597, 2089, 2551, 2561, 2631, 3324, 3688
HgaI GACGC 2 cut(s) 3022, 3556
HhaI GCGC 1 cut(s) 2422
Hin1I GRCGYC 1 cut(s) 1776
Hin6I GCGC 1 cut(s) 2420
HinP1I GCGC 1 cut(s) 2420
HincII GTYRAC 6 cut(s) 775, 806, 824, 968, 1191, 3151
HindII GTYRAC 6 cut(s) 775, 806, 824, 968, 1191, 3151
HindIII AAGCTT 2 cut(s) 2492, 2992
HpaI GTTAAC 2 cut(s) 1191, 3151
HphI GGTGA 9 cut(s) 133, 497, 535, 1069, 1382, 1526, 1811, 2315, 3544
HpyAV CCTTC 4 cut(s) 1649, 2015, 2513, 3699
HpyCH4IV ACGT 1 cut(s) 1776
HpySE526I ACGT 1 cut(s) 1776
Hsp92I GRCGYC 1 cut(s) 1776
HspAI GCGC 1 cut(s) 2420
KflI GGGWCCC 2 cut(s) 359, 1298
Kpn2I TCCGGA 1 cut(s) 2903
KpnI GGTACC 1 cut(s) 835
KroI GCCGGC 1 cut(s) 597
KroNI GCCGGC 1 cut(s) 599
Ksp22I TGATCA 1 cut(s) 3709
KspAI GTTAAC 2 cut(s) 1191, 3151
KspI CCGCGG 1 cut(s) 286
LguI GCTCTTC 1 cut(s) 3158
LmnI GCTCC 8 cut(s) 330, 411, 1034, 1048, 1209, 3074, 3086, 3475
Lsp1109I GCAGC 9 cut(s) 685, 1342, 1636, 1726, 2591, 2625, 2665, 2692, 3641
LweI GCATC 5 cut(s) 566, 2025, 2399, 3493, 3515
MaeII ACGT 1 cut(s) 1776
MaeIII GTNAC 9 cut(s) 276, 485, 524, 541, 956, 1777, 1882, 2303, 3395
MfeI CAATTG 5 cut(s) 887, 1380, 2110, 2466, 2682
MflI RGATCY 3 cut(s) 2382, 2900, 3386
MhlI GDGCHC 3 cut(s) 1053, 3480, 3665
MlsI TGGCCA 1 cut(s) 2551
MluNI TGGCCA 1 cut(s) 2551
MmeI TCCRAC 5 cut(s) 235, 294, 1014, 1161, 3381
Mox20I TGGCCA 1 cut(s) 2551
Mph1103I ATGCAT 2 cut(s) 3418, 3508
MroI TCCGGA 1 cut(s) 2903
MroNI GCCGGC 1 cut(s) 597
MroXI GAANNNNTTC 2 cut(s) 1433, 1760
MscI TGGCCA 1 cut(s) 2551
MslI CAYNNNNRTG 4 cut(s) 2024, 2583, 3237, 3658
Msp20I TGGCCA 1 cut(s) 2551
MspA1I CMGCKG 5 cut(s) 285, 610, 1094, 1955, 3486
MspR9I CCNGG 9 cut(s) 502, 730, 731, 790, 1559, 2186, 2387, 3616, 3677
MunI CAATTG 5 cut(s) 887, 1380, 2110, 2466, 2682
Mva1269I GAATGC 2 cut(s) 1590, 3632
MvaI CCWGG 2 cut(s) 1559, 2186
MvnI CGCG 1 cut(s) 285
NaeI GCCGGC 1 cut(s) 599
NciI CCSGG 7 cut(s) 502, 730, 731, 790, 2387, 3616, 3677
NgoMIV GCCGGC 1 cut(s) 597
NmeAIII GCCGAG 4 cut(s) 429, 573, 685, 2581
NmuCI GTSAC 5 cut(s) 276, 485, 541, 1777, 2303
NsiI ATGCAT 2 cut(s) 3418, 3508
NspI RCATGY 3 cut(s) 219, 2350, 3725
OliI CACNNNNGTG 1 cut(s) 3658
PaeR7I CTCGAG 1 cut(s) 641
PagI TCATGA 1 cut(s) 474
PceI AGGCCT 2 cut(s) 2561, 3688
PciI ACATGT 1 cut(s) 3721
PciSI GCTCTTC 1 cut(s) 3158
PcsI WCGNNNNNNNCGW 1 cut(s) 3428
PctI GAATGC 2 cut(s) 1590, 3632
PdiI GCCGGC 1 cut(s) 599
PdmI GAANNNNTTC 2 cut(s) 1433, 1760
PflFI GACNNNGTC 2 cut(s) 230, 242
PflMI CCANNNNNTGG 1 cut(s) 2249
PfoI TCCNGGA 1 cut(s) 1557
PpuMI RGGWCCY 3 cut(s) 142, 359, 1298
PscI ACATGT 1 cut(s) 3721
Psp124BI GAGCTC 1 cut(s) 3480
Psp5II RGGWCCY 3 cut(s) 142, 359, 1298
Psp6I CCWGG 2 cut(s) 1557, 2184
PspEI GGTNACC 2 cut(s) 524, 541
PspFI CCCAGC 4 cut(s) 1090, 1324, 1951, 2242
PspGI CCWGG 2 cut(s) 1557, 2184
PspPPI RGGWCCY 3 cut(s) 142, 359, 1298
PspXI VCTCGAGB 1 cut(s) 641
PsrI GAACNNNNNNTAC 2 cut(s) 2750, 2782
PstI CTGCAG 1 cut(s) 3660
PsuI RGATCY 3 cut(s) 2382, 2900, 3386
PsyI GACNNNGTC 2 cut(s) 230, 242
PvuII CAGCTG 3 cut(s) 610, 1094, 1955
RsaI GTAC 8 cut(s) 199, 320, 833, 2671, 2768, 2966, 3104, 3642
RsaNI GTAC 8 cut(s) 198, 319, 832, 2670, 2767, 2965, 3103, 3641
RseI CAYNNNNRTG 4 cut(s) 2024, 2583, 3237, 3658
SacI GAGCTC 1 cut(s) 3480
SacII CCGCGG 1 cut(s) 286
SapI GCTCTTC 1 cut(s) 3158
ScaI AGTACT 1 cut(s) 3642
ScrFI CCNGG 9 cut(s) 502, 730, 731, 790, 1559, 2186, 2387, 3616, 3677
SduI GDGCHC 3 cut(s) 1053, 3480, 3665
SfaNI GCATC 5 cut(s) 566, 2025, 2399, 3493, 3515
SfcI CTRYAG 3 cut(s) 2914, 3451, 3656
Sfr274I CTCGAG 1 cut(s) 641
Sfr303I CCGCGG 1 cut(s) 286
SgrBI CCGCGG 1 cut(s) 286
SlaI CTCGAG 1 cut(s) 641
SmaI CCCGGG 1 cut(s) 731
SmiMI CAYNNNNRTG 4 cut(s) 2024, 2583, 3237, 3658
SmlI CTYRAG 6 cut(s) 641, 857, 1835, 2414, 3210, 3425
SmoI CTYRAG 6 cut(s) 641, 857, 1835, 2414, 3210, 3425
SpeI ACTAGT 1 cut(s) 1040
SseBI AGGCCT 2 cut(s) 2561, 3688
SsiI CCGC 8 cut(s) 219, 283, 285, 551, 657, 1568, 2794, 3486
SspI AATATT 1 cut(s) 2697
SstI GAGCTC 1 cut(s) 3480
StuI AGGCCT 2 cut(s) 2561, 3688
StyD4I CCNGG 9 cut(s) 500, 728, 729, 788, 1557, 2184, 2385, 3614, 3675
StyI CCWWGG 2 cut(s) 393, 879
TaiI ACGT 1 cut(s) 1779
TaqII GACCGA 3 cut(s) 220, 232, 593
TatI WGTACW 6 cut(s) 197, 2669, 2766, 2964, 3102, 3640
TseFI GTSAC 5 cut(s) 276, 485, 541, 1777, 2303
Tsp45I GTSAC 5 cut(s) 276, 485, 541, 1777, 2303
TspDTI ATGAA 7 cut(s) 1478, 1621, 1749, 1953, 2652, 3681, 3773
TspGWI ACGGA 2 cut(s) 3129, 3515
TspMI CCCGGG 1 cut(s) 729
Tth111I GACNNNGTC 2 cut(s) 230, 242
Van91I CCANNNNNTGG 1 cut(s) 2249
VneI GTGCAC 1 cut(s) 3661
XagI CCTNNNNNAGG 2 cut(s) 1242, 2049
XceI RCATGY 3 cut(s) 219, 2350, 3725
XcmI CCANNNNNNNNNTGG 1 cut(s) 2835
XhoI CTCGAG 1 cut(s) 641
XmaI CCCGGG 1 cut(s) 729
XmiI GTMKAC 3 cut(s) 2913, 2930, 3450
XmnI GAANNNNTTC 2 cut(s) 1433, 1760
ZraI GACGTC 1 cut(s) 1777
ZrmI AGTACT 1 cut(s) 3642
Zsp2I ATGCAT 2 cut(s) 3418, 3508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.