AT4G22600

Seed dormancy control

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0016591
Physical Location & Seq
Reverse (-)
11900211 .. 11901032
822 bp
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UTR
Exon/CDS
Intron
AT4G22600.1

Sequence Viewer

Length: 822 bp
ATGCCTTTCTCTTTCTTCTCCCGCAAGAAACCATCTCGTCGATTCAATGATTTCTACGAAGACTGGTCAAAAACCCTAACCGAAAACTGCCTCCCTCTCCTCCGTCAATCACTTTCCTCCGCCGCTTCCGCTTCCGTCCTCTCCTCCAACGTAGACCTCGTCCTCCGTCACCTAGTCTTATACTACGAAACCCTAGATCTTGCTGCCGATCACAACACCATTCCTTACCTTCTCTTCCCTTCATGGCGTAACTCTCTCGAGACTCCGTTTCTTTTCCTCGGCGATATCCATCCTTACCTCCTCACCAATCTCCTCCGTTCTTTCATCGACCGTGAGAATCAAGATTCCGACGACGAAGACGAGGAAACTTCTCTTGATCTTGTGAATCAACCGTTAAAGATGACGATGGCGTGGAAAGATCCTTCTGATGAGTTGGTTAAAAGAATCGATCAGATTGAGTGTACAATGAGACTTATGGTTCCTGGTTTAATGGATCGTATGAGGAAAACACAGAGAAGTTTCGTGGCTCGAGTTTCTGAGAGTTGGGTCTCGTCGTATCAAGTTGGGAAGAAGAAGAAGCTTACTGCGACGGTTGCGACGGCTTCAACGTCGGTGGATGAAGCAGCAAAGATTGAGATGGAAGAGCTTGTGAGTATTTTCGTTGATGCGAATCGTTTGAGGAAGAGTGTGATTATGGACATTGTTGGAGCTACAAGTGAGCATCAAGCGGCTCTGTTTCTTGAGGGTTTATGTCAGTTTCTTGCTGGGTTTAAAGATCAGATTCTTCTTCAAGATTTTGAGATTCTTTCTTTGCCCAATTAA

Protein Analysis

273

Amino Acids

31.25

Weight (kDa)

5.06

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 31 - 107 1.8e-15 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016591)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22600
fragaria_vesca FvH4_5g00261
malus_domestica MD06G1063700.v1.1
prunus_persica Prupe.5G075300_v2.0.a1
pyrus_communis pycom06g05990
rosa_chinensis RchiOBHm_Chr7g0202371
rosa_laevigata RLG00000003606
rosa_multiflora Rmu_sc0000266.1_g000010
rosa_rugosa Rorug07G0070100
rosa_samantha Rh7AG197600 Rh7BG198600 Rh7CG208700 Rh7DG204400
rosa_wichuraiana Rw7G017320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 153
AciI CCGC 5 cut(s) 22, 120, 123, 129, 728
AclWI GGATC 2 cut(s) 413, 501
AfaI GTAC 1 cut(s) 463
AgsI TTSAA 3 cut(s) 46, 606, 791
AjnI CCWGG 1 cut(s) 481
AluBI AGCT 3 cut(s) 580, 646, 710
AluI AGCT 3 cut(s) 580, 646, 710
Alw26I GTCTC 3 cut(s) 254, 463, 553
AlwI GGATC 2 cut(s) 413, 501
Ama87I CYCGRG 2 cut(s) 257, 528
ApeKI GCWGC 2 cut(s) 203, 623
AsuHPI GGTGA 2 cut(s) 161, 295
AvaI CYCGRG 2 cut(s) 257, 528
BbsI GAAGAC 2 cut(s) 66, 363
BbvI GCAGC 2 cut(s) 190, 635
BccI CCATC 4 cut(s) 40, 297, 400, 631
BceAI ACGGC 1 cut(s) 615
BciT130I CCWGG 1 cut(s) 483
BcoDI GTCTC 3 cut(s) 254, 463, 553
BfaI CTAG 2 cut(s) 173, 194
BglII AGATCT 1 cut(s) 196
BisI GCNGC 4 cut(s) 123, 204, 624, 729
BlsI GCNGC 4 cut(s) 124, 205, 625, 730
Bme1390I CCNGG 1 cut(s) 483
BmeT110I CYCGRG 2 cut(s) 257, 528
BmiI GGNNCC 1 cut(s) 480
BmrFI CCNGG 1 cut(s) 483
BmsI GCATC 2 cut(s) 655, 730
BpiI GAAGAC 2 cut(s) 66, 363
BpuEI CTTGAG 1 cut(s) 761
Bsa29I ATCGAT 1 cut(s) 447
BsaBI GATNNNNATC 2 cut(s) 288, 669
BsaI GGTCTC 1 cut(s) 553
BsaJI CCNNGG 1 cut(s) 277
Bse1I ACTGG 1 cut(s) 68
Bse8I GATNNNNATC 2 cut(s) 288, 669
BseBI CCWGG 1 cut(s) 483
BseCI ATCGAT 1 cut(s) 447
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 2 cut(s) 289, 622
BseJI GATNNNNATC 2 cut(s) 288, 669
BseMII CTCAG 1 cut(s) 528
BseNI ACTGG 1 cut(s) 68
BseRI GAGGAG 4 cut(s) 89, 133, 290, 302
BseXI GCAGC 2 cut(s) 190, 635
BseYI CCCAGC 1 cut(s) 764
Bsh1285I CGRYCG 1 cut(s) 331
BshVI ATCGAT 1 cut(s) 447
BsiEI CGRYCG 1 cut(s) 331
BsiHKCI CYCGRG 2 cut(s) 257, 528
BsmAI GTCTC 3 cut(s) 254, 463, 553
Bso31I GGTCTC 1 cut(s) 553
BsoBI CYCGRG 2 cut(s) 257, 528
Bsp1407I TGTACA 1 cut(s) 461
Bsp143I GATC 7 cut(s) 196, 208, 376, 418, 448, 493, 775
BspACI CCGC 5 cut(s) 22, 120, 123, 129, 728
BspCNI CTCAG 1 cut(s) 529
BspDI ATCGAT 1 cut(s) 447
BspLI GGNNCC 1 cut(s) 480
BspPI GGATC 2 cut(s) 413, 501
BspQI GCTCTTC 1 cut(s) 636
BspTNI GGTCTC 1 cut(s) 553
BsrGI TGTACA 1 cut(s) 461
BsrI ACTGG 1 cut(s) 68
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 7 cut(s) 196, 208, 376, 418, 448, 493, 775
Bst2UI CCWGG 1 cut(s) 483
Bst4CI ACNGT 3 cut(s) 332, 393, 592
Bst6I CTCTTC 3 cut(s) 239, 636, 677
BstAUI TGTACA 1 cut(s) 461
BstDEI CTNAG 1 cut(s) 537
BstF5I GGATG 2 cut(s) 289, 622
BstKTI GATC 7 cut(s) 199, 211, 379, 421, 451, 496, 778
BstMAI GTCTC 3 cut(s) 254, 463, 553
BstMBI GATC 7 cut(s) 196, 208, 376, 418, 448, 493, 775
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 2 cut(s) 128, 593
BstNI CCWGG 1 cut(s) 483
BstSCI CCNGG 1 cut(s) 481
BstV1I GCAGC 2 cut(s) 190, 635
BstV2I GAAGAC 2 cut(s) 66, 363
BstX2I RGATCY 2 cut(s) 196, 418
BstYI RGATCY 2 cut(s) 196, 418
Bsu15I ATCGAT 1 cut(s) 447
BsuTUI ATCGAT 1 cut(s) 447
BtsCI GGATG 2 cut(s) 289, 622
ClaI ATCGAT 1 cut(s) 447
Csp6I GTAC 1 cut(s) 462
CspCI CAANNNNNGTGG 2 cut(s) 594, 629
CviAII CATG 1 cut(s) 243
CviJI RGCY 6 cut(s) 527, 580, 602, 646, 710, 731
CviKI_1 RGCY 6 cut(s) 527, 580, 602, 646, 710, 731
CviQI GTAC 1 cut(s) 462
DdeI CTNAG 1 cut(s) 537
DpnI GATC 7 cut(s) 198, 210, 378, 420, 450, 495, 777
DpnII GATC 7 cut(s) 196, 208, 376, 418, 448, 493, 775
DraI TTTAAA 1 cut(s) 772
Eam1104I CTCTTC 3 cut(s) 239, 636, 677
EarI CTCTTC 3 cut(s) 239, 636, 677
EciI GGCGGA 1 cut(s) 109
Eco31I GGTCTC 1 cut(s) 553
Eco32I GATATC 1 cut(s) 286
Eco88I CYCGRG 2 cut(s) 257, 528
EcoRII CCWGG 1 cut(s) 481
EcoRV GATATC 1 cut(s) 286
FaeI CATG 1 cut(s) 246
FaiI YATR 6 cut(s) 181, 244, 476, 500, 695, 751
FatI CATG 1 cut(s) 242
FauI CCCGC 1 cut(s) 29
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 4 cut(s) 123, 204, 624, 729
FokI GGATG 2 cut(s) 276, 629
Fsp4HI GCNGC 4 cut(s) 123, 204, 624, 729
FspBI CTAG 2 cut(s) 173, 194
GluI GCNGC 4 cut(s) 123, 204, 624, 729
GsaI CCCAGC 1 cut(s) 768
Hin1II CATG 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 9 cut(s) 42, 262, 337, 344, 385, 444, 670, 781, 802
HphI GGTGA 2 cut(s) 161, 295
Hpy166II GTNNAC 2 cut(s) 154, 462
Hpy188I TCNGA 5 cut(s) 349, 427, 453, 538, 780
Hpy188III TCNNGA 6 cut(s) 257, 259, 341, 374, 740, 791
Hpy8I GTNNAC 2 cut(s) 154, 462
Hpy99I CGWCG 7 cut(s) 42, 353, 356, 556, 592, 601, 613
HpyAV CCTTC 3 cut(s) 239, 249, 432
HpyCH4III ACNGT 3 cut(s) 332, 393, 592
HpyCH4IV ACGT 2 cut(s) 150, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 593
HpyF3I CTNAG 1 cut(s) 537
HpySE526I ACGT 2 cut(s) 150, 608
Hsp92II CATG 1 cut(s) 246
Kzo9I GATC 7 cut(s) 196, 208, 376, 418, 448, 493, 775
LguI GCTCTTC 1 cut(s) 636
LmnI GCTCC 1 cut(s) 707
LpnPI CCDG 4 cut(s) 49, 468, 495, 750
Lsp1109I GCAGC 2 cut(s) 190, 635
LweI GCATC 2 cut(s) 655, 730
MaeI CTAG 2 cut(s) 173, 194
MaeII ACGT 2 cut(s) 150, 608
MaeIII GTNAC 2 cut(s) 167, 248
MalI GATC 7 cut(s) 198, 210, 378, 420, 450, 495, 777
MboI GATC 7 cut(s) 196, 208, 376, 418, 448, 493, 775
MflI RGATCY 2 cut(s) 196, 418
MluCI AATT 1 cut(s) 817
MlyI GAGTC 1 cut(s) 256
MmeI TCCRAC 3 cut(s) 171, 372, 685
MseI TTAA 5 cut(s) 395, 438, 488, 771, 820
MspR9I CCNGG 1 cut(s) 483
MvaI CCWGG 1 cut(s) 483
MwoI GCNNNNNNNGC 2 cut(s) 128, 593
NdeII GATC 7 cut(s) 196, 208, 376, 418, 448, 493, 775
NlaIII CATG 1 cut(s) 246
NlaIV GGNNCC 1 cut(s) 480
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 167
PaeR7I CTCGAG 2 cut(s) 257, 528
PciSI GCTCTTC 1 cut(s) 636
PcsI WCGNNNNNNNCGW 3 cut(s) 156, 357, 605
PfeI GAWTC 8 cut(s) 42, 337, 344, 385, 444, 670, 781, 802
PflFI GACNNNGTC 1 cut(s) 158
PkrI GCNGC 4 cut(s) 124, 205, 625, 730
PleI GAGTC 1 cut(s) 256
PpsI GAGTC 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 481
PspFI CCCAGC 1 cut(s) 764
PspGI CCWGG 1 cut(s) 481
PspN4I GGNNCC 1 cut(s) 480
PspXI VCTCGAGB 1 cut(s) 528
PsuI RGATCY 2 cut(s) 196, 418
PsyI GACNNNGTC 1 cut(s) 158
RsaI GTAC 1 cut(s) 463
RsaNI GTAC 1 cut(s) 462
SapI GCTCTTC 1 cut(s) 636
SaqAI TTAA 5 cut(s) 395, 438, 488, 771, 820
SatI GCNGC 4 cut(s) 123, 204, 624, 729
Sau3AI GATC 7 cut(s) 196, 208, 376, 418, 448, 493, 775
SchI GAGTC 1 cut(s) 256
ScrFI CCNGG 1 cut(s) 483
SetI ASST 9 cut(s) 153, 159, 174, 231, 300, 582, 611, 648, 712
SfaNI GCATC 2 cut(s) 655, 730
Sfr274I CTCGAG 2 cut(s) 257, 528
SlaI CTCGAG 2 cut(s) 257, 528
SmlI CTYRAG 3 cut(s) 257, 528, 740
SmoI CTYRAG 3 cut(s) 257, 528, 740
Sse9I AATT 1 cut(s) 817
SsiI CCGC 5 cut(s) 22, 120, 123, 129, 728
SspMI CTAG 2 cut(s) 173, 194
StyD4I CCNGG 1 cut(s) 481
TaaI ACNGT 3 cut(s) 332, 393, 592
TaiI ACGT 2 cut(s) 153, 611
TaqI TCGA 5 cut(s) 40, 258, 327, 447, 529
TasI AATT 1 cut(s) 817
TatI WGTACW 1 cut(s) 461
TauI GCSGC 2 cut(s) 125, 731
TfiI GAWTC 8 cut(s) 42, 337, 344, 385, 444, 670, 781, 802
Tru1I TTAA 5 cut(s) 395, 438, 488, 771, 820
Tru9I TTAA 5 cut(s) 395, 438, 488, 771, 820
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 2 cut(s) 203, 623
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 3 cut(s) 231, 313, 633
TspGWI ACGGA 5 cut(s) 92, 124, 155, 255, 305
Tth111I GACNNNGTC 1 cut(s) 158
XhoI CTCGAG 2 cut(s) 257, 528
XmiI GTMKAC 1 cut(s) 153
XspI CTAG 2 cut(s) 173, 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.