MD06G1063700.v1.1

Seed dormancy control

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016591
Physical Location & Seq
Forward (+)
10981758 .. 10983302
1545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1063700.v1.1.491

Sequence Viewer

Length: 879 bp
ATGCTCAAAGCCGTAGGTCTCTTCGGCCGGAAGAAAAGCGGCAGCAGCGGCGGCAACCGCCATTTCAAAGACTTCTACGCCGACTGGTTCAACACACTCAATACCACGCTCCTCCCTTCGCTTCAGAACTCGATGTCCGAGTCCGACTCGTCCCTGACTCACCTCTCCACTCAGGTCGAAACCCTCCATCGCCACTTCCAATCCTACTACGACGCCCTCGACCTCGCCGCCGCAAACGACGTCGCTCAGCTCCTTGACCCCGACTGGCGAAACCCTCTCGAGAAGCCCTTCCTCTTCCTCGGCGACCTCCACCCCTACCTCTTCACCAACCTCGTCCGCTCTTTCCTCAACCAAAACGACTCCGACGACGATGAAGACTACGACAACGACAGCCTAACCGTTCAATTCGCCGACGCCCAAGACAGAGACAGGGACCAACTCGAAGTCCTCTTCGACCGCCCTTGGCACATCGCGACGGCGTGGAGGAGTCAGTCGTACGATCTGATGGGCAAGCTCGAGCAGGTCGAGCGCGGGCTGAGGCTGATGGTGCCGGCGCTGGTGGCGCGCGCGAGGGACGCTCAGGCCGCGTTCTTGGAGCACGTGGGGAGGAATTGGGATTATGGTAGCGGTCTGCAGAAGCCGGCGGTGGCGGAAGCTATGGCGGAGCAGAATGAGGAGATGGTGGGGGTTTTTGTGGATGCGAATCGGCTGAGAAGGAGTGTTCTTGTGGAGATCCTTGGCACCACCAGCGTGTTCCAGGCGGCTCTGTTTCTTGAAGGGTTGGCCCAATTTCTTGTTGGGTTCAGAGACCCTGAGTTACTTGCTCAGTTTGATCTCTGCAAAACGCCTCTCGGGAAGCAGAATCGCTTGGCGATTTGA

Protein Analysis

293

Amino Acids

33.08

Weight (kDa)

4.88

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 38 - 114 3.5e-18 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016591)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22600
fragaria_vesca FvH4_5g00261
malus_domestica MD06G1063700.v1.1
prunus_persica Prupe.5G075300_v2.0.a1
pyrus_communis pycom06g05990
rosa_chinensis RchiOBHm_Chr7g0202371
rosa_laevigata RLG00000003606
rosa_multiflora Rmu_sc0000266.1_g000010
rosa_rugosa Rorug07G0070100
rosa_samantha Rh7AG197600 Rh7BG198600 Rh7CG208700 Rh7DG204400
rosa_wichuraiana Rw7G017320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 243
Acc36I ACCTGC 1 cut(s) 511
AccB1I GGYRCC 2 cut(s) 547, 740
AccBSI CCGCTC 1 cut(s) 339
AccII CGCG 6 cut(s) 473, 531, 565, 567, 569, 587
AclWI GGATC 1 cut(s) 727
AcoI YGGCCR 1 cut(s) 25
AcuI CTGAAG 1 cut(s) 107
AcvI CACGTG 1 cut(s) 601
AcyI GRCGYC 3 cut(s) 213, 240, 414
AfaI GTAC 1 cut(s) 497
AgsI TTSAA 4 cut(s) 67, 91, 404, 776
AjnI CCWGG 1 cut(s) 756
AleI CACNNNNGTG 1 cut(s) 749
AloI GAACNNNNNNTCC 2 cut(s) 119, 151
AluBI AGCT 3 cut(s) 250, 514, 656
AluI AGCT 3 cut(s) 250, 514, 656
Alw21I GWGCWC 1 cut(s) 600
Alw26I GTCTC 3 cut(s) 23, 420, 801
AlwI GGATC 1 cut(s) 727
Ama87I CYCGRG 3 cut(s) 278, 515, 851
AoxI GGCC 3 cut(s) 25, 582, 783
ApeKI GCWGC 2 cut(s) 42, 45
Asp700I GAANNNNTTC 1 cut(s) 287
AspLEI GCGC 5 cut(s) 531, 556, 565, 567, 569
AspS9I GGNCC 2 cut(s) 433, 784
AsuHPI GGTGA 2 cut(s) 152, 316
AvaI CYCGRG 3 cut(s) 278, 515, 851
AvaII GGWCC 1 cut(s) 433
BanI GGYRCC 2 cut(s) 547, 740
BbrPI CACGTG 1 cut(s) 601
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 600
BbvCI CCTCAGC 1 cut(s) 536
BbvI GCAGC 2 cut(s) 54, 57
BccI CCATC 4 cut(s) 195, 499, 538, 673
BceAI ACGGC 1 cut(s) 492
BciT130I CCWGG 1 cut(s) 758
BcoDI GTCTC 3 cut(s) 23, 420, 801
BfmI CTRYAG 1 cut(s) 632
BfoI RGCGCY 1 cut(s) 557
BfuAI ACCTGC 1 cut(s) 511
BisI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 228, 231, 585, 762
BlpI GCTNAGC 1 cut(s) 246
BlsI GCNGC 9 cut(s) 41, 44, 47, 50, 53, 229, 232, 586, 763
Bme1390I CCNGG 1 cut(s) 758
Bme18I GGWCC 1 cut(s) 433
BmeT110I CYCGRG 3 cut(s) 278, 515, 851
BmgT120I GGNCC 2 cut(s) 433, 784
BmiI GGNNCC 3 cut(s) 434, 549, 742
BmrFI CCNGG 1 cut(s) 758
BmsI GCATC 1 cut(s) 688
BpiI GAAGAC 1 cut(s) 381
BplI GAGNNNNNCTC 4 cut(s) 131, 163, 562, 594
Bpu10I CCTNAGC 2 cut(s) 536, 579
Bpu1102I GCTNAGC 1 cut(s) 246
BsaAI YACGTR 1 cut(s) 601
BsaBI GATNNNNATC 1 cut(s) 702
BsaHI GRCGYC 3 cut(s) 213, 240, 414
BsaI GGTCTC 2 cut(s) 23, 801
BsaJI CCNNGG 3 cut(s) 298, 461, 736
Bse118I RCCGGY 2 cut(s) 550, 640
Bse1I ACTGG 2 cut(s) 89, 269
Bse8I GATNNNNATC 1 cut(s) 702
BseBI CCWGG 1 cut(s) 758
BseDI CCNNGG 3 cut(s) 298, 461, 736
BseGI GGATG 1 cut(s) 703
BseJI GATNNNNATC 1 cut(s) 702
BseMII CTCAG 7 cut(s) 185, 260, 527, 593, 701, 804, 839
BseNI ACTGG 2 cut(s) 89, 269
BsePI GCGCGC 2 cut(s) 563, 565
BseRI GAGGAG 3 cut(s) 101, 499, 689
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 2 cut(s) 54, 57
Bsh1236I CGCG 6 cut(s) 473, 531, 565, 567, 569, 587
Bsh1285I CGRYCG 2 cut(s) 28, 457
BshFI GGCC 3 cut(s) 27, 584, 785
BshNI GGYRCC 2 cut(s) 547, 740
BsiEI CGRYCG 2 cut(s) 28, 457
BsiHKAI GWGCWC 1 cut(s) 600
BsiHKCI CYCGRG 3 cut(s) 278, 515, 851
BsiSI CCGG 3 cut(s) 28, 551, 641
BsiWI CGTACG 1 cut(s) 495
BslFI GGGAC 3 cut(s) 136, 446, 587
BsmAI GTCTC 3 cut(s) 23, 420, 801
BsmFI GGGAC 3 cut(s) 136, 446, 587
BsnI GGCC 3 cut(s) 27, 584, 785
Bso31I GGTCTC 2 cut(s) 23, 801
BsoBI CYCGRG 3 cut(s) 278, 515, 851
Bsp1286I GDGCHC 1 cut(s) 600
Bsp143I GATC 3 cut(s) 499, 732, 832
Bsp1720I GCTNAGC 1 cut(s) 246
Bsp68I TCGCGA 1 cut(s) 473
BspANI GGCC 3 cut(s) 27, 584, 785
BspCNI CTCAG 7 cut(s) 184, 259, 528, 592, 702, 805, 838
BspFNI CGCG 6 cut(s) 473, 531, 565, 567, 569, 587
BspLI GGNNCC 3 cut(s) 434, 549, 742
BspMAI CTGCAG 1 cut(s) 636
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 1 cut(s) 727
BspT107I GGYRCC 2 cut(s) 547, 740
BspTNI GGTCTC 2 cut(s) 23, 801
BsrBI CCGCTC 1 cut(s) 339
BsrFI RCCGGY 2 cut(s) 550, 640
BsrI ACTGG 2 cut(s) 89, 269
BssAI RCCGGY 2 cut(s) 550, 640
BssECI CCNNGG 3 cut(s) 298, 461, 736
BssHII GCGCGC 2 cut(s) 563, 565
BssMI GATC 3 cut(s) 499, 732, 832
BssNI GRCGYC 3 cut(s) 213, 240, 414
BssT1I CCWWGG 2 cut(s) 461, 736
Bst2UI CCWGG 1 cut(s) 758
Bst4CI ACNGT 1 cut(s) 400
Bst6I CTCTTC 4 cut(s) 26, 299, 326, 455
BstACI GRCGYC 3 cut(s) 213, 240, 414
BstBAI YACGTR 1 cut(s) 601
BstC8I GCNNGC 6 cut(s) 512, 533, 552, 565, 567, 642
BstDEI CTNAG 7 cut(s) 171, 246, 536, 579, 710, 813, 825
BstF5I GGATG 1 cut(s) 703
BstFNI CGCG 6 cut(s) 473, 531, 565, 567, 569, 587
BstH2I RGCGCY 1 cut(s) 557
BstHHI GCGC 5 cut(s) 531, 556, 565, 567, 569
BstKTI GATC 3 cut(s) 502, 735, 835
BstMAI GTCTC 3 cut(s) 23, 420, 801
BstMBI GATC 3 cut(s) 499, 732, 832
BstMCI CGRYCG 2 cut(s) 28, 457
BstNI CCWGG 1 cut(s) 758
BstSCI CCNGG 1 cut(s) 756
BstSFI CTRYAG 1 cut(s) 632
BstUI CGCG 6 cut(s) 473, 531, 565, 567, 569, 587
BstV1I GCAGC 2 cut(s) 54, 57
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 1 cut(s) 732
BstYI RGATCY 1 cut(s) 732
BstZI CGGCCG 1 cut(s) 25
BsuRI GGCC 3 cut(s) 27, 584, 785
BtgZI GCGATG 2 cut(s) 173, 454
BtsCI GGATG 1 cut(s) 703
BtuMI TCGCGA 1 cut(s) 473
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 6 cut(s) 512, 533, 552, 565, 567, 642
CfoI GCGC 5 cut(s) 531, 556, 565, 567, 569
Cfr10I RCCGGY 2 cut(s) 550, 640
Cfr13I GGNCC 2 cut(s) 433, 784
CseI GACGC 3 cut(s) 221, 422, 584
Csp6I GTAC 1 cut(s) 496
CviQI GTAC 1 cut(s) 496
DdeI CTNAG 7 cut(s) 171, 246, 536, 579, 710, 813, 825
DpnI GATC 3 cut(s) 501, 734, 834
DpnII GATC 3 cut(s) 499, 732, 832
EaeI YGGCCR 1 cut(s) 25
EagI CGGCCG 1 cut(s) 25
Eam1104I CTCTTC 4 cut(s) 26, 299, 326, 455
EarI CTCTTC 4 cut(s) 26, 299, 326, 455
EciI GGCGGA 2 cut(s) 665, 677
EclXI CGGCCG 1 cut(s) 25
Eco130I CCWWGG 2 cut(s) 461, 736
Eco31I GGTCTC 2 cut(s) 23, 801
Eco47I GGWCC 1 cut(s) 433
Eco52I CGGCCG 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 107
Eco72I CACGTG 1 cut(s) 601
Eco88I CYCGRG 3 cut(s) 278, 515, 851
EcoRII CCWGG 1 cut(s) 756
EcoT14I CCWWGG 2 cut(s) 461, 736
ErhI CCWWGG 2 cut(s) 461, 736
FaiI YATR 2 cut(s) 621, 659
FaqI GGGAC 3 cut(s) 136, 446, 587
FauI CCCGC 1 cut(s) 524
Fnu4HI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 228, 231, 585, 762
FokI GGATG 1 cut(s) 710
Fsp4HI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 228, 231, 585, 762
GlaI GCGC 5 cut(s) 530, 555, 564, 566, 568
GluI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 228, 231, 585, 762
HaeII RGCGCY 1 cut(s) 557
HaeIII GGCC 3 cut(s) 27, 584, 785
HapII CCGG 3 cut(s) 28, 551, 641
HgaI GACGC 3 cut(s) 221, 422, 584
HhaI GCGC 5 cut(s) 531, 556, 565, 567, 569
Hin1I GRCGYC 3 cut(s) 213, 240, 414
Hin6I GCGC 5 cut(s) 529, 554, 563, 565, 567
HinP1I GCGC 5 cut(s) 529, 554, 563, 565, 567
HinfI GANTC 7 cut(s) 140, 146, 157, 359, 487, 703, 862
HpaII CCGG 3 cut(s) 28, 551, 641
HphI GGTGA 2 cut(s) 152, 316
Hpy188I TCNGA 6 cut(s) 126, 139, 145, 364, 504, 806
Hpy188III TCNNGA 5 cut(s) 278, 280, 472, 773, 853
Hpy99I CGWCG 7 cut(s) 215, 242, 245, 368, 371, 416, 478
HpyAV CCTTC 4 cut(s) 126, 298, 708, 770
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 2 cut(s) 240, 600
HpyCH4V TGCA 2 cut(s) 634, 840
HpyF3I CTNAG 7 cut(s) 171, 246, 536, 579, 710, 813, 825
HpySE526I ACGT 2 cut(s) 240, 600
Hsp92I GRCGYC 3 cut(s) 213, 240, 414
HspAI GCGC 5 cut(s) 529, 554, 563, 565, 567
KroI GCCGGC 2 cut(s) 550, 640
KroNI GCCGGC 2 cut(s) 552, 642
Kzo9I GATC 3 cut(s) 499, 732, 832
LmnI GCTCC 4 cut(s) 114, 255, 595, 664
Lsp1109I GCAGC 2 cut(s) 54, 57
LweI GCATC 1 cut(s) 688
MaeII ACGT 2 cut(s) 240, 600
MaeIII GTNAC 1 cut(s) 816
MalI GATC 3 cut(s) 501, 734, 834
MauBI CGCGCGCG 1 cut(s) 565
MbiI CCGCTC 1 cut(s) 339
MboI GATC 3 cut(s) 499, 732, 832
MboII GAAGA 6 cut(s) 13, 43, 286, 313, 386, 442
MflI RGATCY 1 cut(s) 732
MhlI GDGCHC 1 cut(s) 600
MluCI AATT 3 cut(s) 404, 610, 788
MlyI GAGTC 5 cut(s) 140, 149, 151, 353, 496
MmeI TCCRAC 2 cut(s) 168, 387
MroNI GCCGGC 2 cut(s) 550, 640
MroXI GAANNNNTTC 1 cut(s) 287
MslI CAYNNNNRTG 1 cut(s) 749
MspA1I CMGCKG 1 cut(s) 48
MspI CCGG 3 cut(s) 28, 551, 641
MspR9I CCNGG 1 cut(s) 758
MvaI CCWGG 1 cut(s) 758
MvnI CGCG 6 cut(s) 473, 531, 565, 567, 569, 587
NaeI GCCGGC 2 cut(s) 552, 642
NdeII GATC 3 cut(s) 499, 732, 832
NgoMIV GCCGGC 2 cut(s) 550, 640
NlaIV GGNNCC 3 cut(s) 434, 549, 742
NmeAIII GCCGAG 1 cut(s) 279
NruI TCGCGA 1 cut(s) 473
OliI CACNNNNGTG 1 cut(s) 749
PaeR7I CTCGAG 2 cut(s) 278, 515
PauI GCGCGC 2 cut(s) 563, 565
PcsI WCGNNNNNNNCGW 3 cut(s) 216, 363, 522
PdiI GCCGGC 2 cut(s) 552, 642
PdmI GAANNNNTTC 1 cut(s) 287
PfeI GAWTC 2 cut(s) 703, 862
Pfl23II CGTACG 1 cut(s) 495
PkrI GCNGC 9 cut(s) 41, 44, 47, 50, 53, 229, 232, 586, 763
PleI GAGTC 5 cut(s) 140, 148, 151, 353, 495
PmaCI CACGTG 1 cut(s) 601
PmlI CACGTG 1 cut(s) 601
PpsI GAGTC 5 cut(s) 140, 148, 151, 353, 495
Ppu21I YACGTR 1 cut(s) 601
Psp6I CCWGG 1 cut(s) 756
PspCI CACGTG 1 cut(s) 601
PspGI CCWGG 1 cut(s) 756
PspLI CGTACG 1 cut(s) 495
PspN4I GGNNCC 3 cut(s) 434, 549, 742
PspPI GGNCC 2 cut(s) 433, 784
PspXI VCTCGAGB 1 cut(s) 515
PstI CTGCAG 1 cut(s) 636
PsuI RGATCY 1 cut(s) 732
PteI GCGCGC 2 cut(s) 563, 565
RruI TCGCGA 1 cut(s) 473
RsaI GTAC 1 cut(s) 497
RsaNI GTAC 1 cut(s) 496
RseI CAYNNNNRTG 1 cut(s) 749
SatI GCNGC 9 cut(s) 40, 43, 46, 49, 52, 228, 231, 585, 762
Sau3AI GATC 3 cut(s) 499, 732, 832
Sau96I GGNCC 2 cut(s) 433, 784
SchI GAGTC 5 cut(s) 140, 149, 151, 353, 496
ScrFI CCNGG 1 cut(s) 758
SduI GDGCHC 1 cut(s) 600
SfaNI GCATC 1 cut(s) 688
SfcI CTRYAG 1 cut(s) 632
Sfr274I CTCGAG 2 cut(s) 278, 515
SinI GGWCC 1 cut(s) 433
SlaI CTCGAG 2 cut(s) 278, 515
SmiMI CAYNNNNRTG 1 cut(s) 749
SmlI CTYRAG 2 cut(s) 278, 515
SmoI CTYRAG 2 cut(s) 278, 515
Sse9I AATT 3 cut(s) 404, 610, 788
StyD4I CCNGG 1 cut(s) 756
StyI CCWWGG 2 cut(s) 461, 736
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 2 cut(s) 243, 603
TaqI TCGA 8 cut(s) 131, 177, 219, 279, 441, 453, 516, 525
TasI AATT 3 cut(s) 404, 610, 788
TauI GCSGC 7 cut(s) 42, 51, 54, 230, 233, 587, 764
TfiI GAWTC 2 cut(s) 703, 862
TseI GCWGC 2 cut(s) 42, 45
TspDTI ATGAA 1 cut(s) 387
VpaK11BI GGWCC 1 cut(s) 433
XcmI CCANNNNNNNNNTGG 1 cut(s) 794
XhoI CTCGAG 2 cut(s) 278, 515
XmnI GAANNNNTTC 1 cut(s) 287
ZraI GACGTC 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.