Rh7AG197600

Seed dormancy control

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0016591
Physical Location & Seq
Reverse (-)
17821000 .. 17822659
1660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG197600.1

Sequence Viewer

Length: 840 bp
ATGTTGAAAGCAGTAGGTCTTTTCGGCCGCAGCAGCAACAACAAGAAGAGCCCCCGGCCCTTCAAGGATTACTACTCCGAATGGCTGACCACCCTCAAGTCCTCCCTCCTCCCCTCCCTCCGCCAATCCCTCTCCGACGCCTCCTCCCTCCCAAACCTCTCCCTCCACGTCGACGCCCTCCACCGCCACTTCCTCTCCTACTACGACGCCCTCGACTCCGCCGCCCAGTCCGACGTCGCCCAGCTCCTCTTCCCCGACTGGCGCAACCCTCTCGAGAAGCCCTTCCTCTGGCTCGCCGACCTCCACCCCTACCTCTTCACCAACCTCCTCCGCTCCTTCCTCGACTCCGCCTCCGACGAAGATTCCGACGCCGACTCCGACAGCCTCGTCGTCCAGTTCGGCGACCGCCCCTGGCACATCGCCACCGCCTGGCGCAGCCCTTCCTCTTCTCTCACGCAAAAGGTCGAGCAGGTGGAGTGCGGTCTCAGGCTCATGGTGCCTGCGCTGGCGGCGCGTGCGAGGGACGGGCAGGCCGCGTTCGTGGAGCGCGTGGCGAGGAACTGGGGGCCTTACGACGCCGGGAAGAAGGAGGCGGCGAAGGCGGTGATGGCGGAGGCGATGCAGGCGGAGATGGAGGAGATGGTGAGCGTTCTGGTGGATGCGAATAGGCTGAGGAGGAGTGTTCTTGCTGAGATTGTTGGGGCCGCCAGTGTTTACCAGGCCGCTCTGTTTCTTGAAGGCCTGGCCCAATTTCTTGTTGGGTTCAGAAACCCGGAATTGCTTGCTCAGTTTGATCAGTGCAAGACTCCTCTCAGTAAGCAATGCCGCTTGGCTATTTGA

Protein Analysis

279

Amino Acids

31.1

Weight (kDa)

5.99

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 37 - 112 7.4e-20 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016591)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22600
fragaria_vesca FvH4_5g00261
malus_domestica MD06G1063700.v1.1
prunus_persica Prupe.5G075300_v2.0.a1
pyrus_communis pycom06g05990
rosa_chinensis RchiOBHm_Chr7g0202371
rosa_laevigata RLG00000003606
rosa_multiflora Rmu_sc0000266.1_g000010
rosa_rugosa Rorug07G0070100
rosa_samantha Rh7AG197600 Rh7BG198600 Rh7CG208700 Rh7DG204400
rosa_wichuraiana Rw7G017320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 460
AasI GACNNNNNNGTC 1 cut(s) 386
AatII GACGTC 1 cut(s) 237
Acc36I ACCTGC 1 cut(s) 460
AccB1I GGYRCC 1 cut(s) 496
AccB7I CCANNNNNTGG 1 cut(s) 429
AccBSI CCGCTC 2 cut(s) 333, 725
AccI GTMKAC 1 cut(s) 171
AccII CGCG 3 cut(s) 514, 536, 549
AcoI YGGCCR 1 cut(s) 25
AcyI GRCGYC 6 cut(s) 138, 174, 207, 234, 369, 576
AfiI CCNNNNNNNGG 2 cut(s) 288, 429
AgsI TTSAA 3 cut(s) 7, 64, 737
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 4 cut(s) 410, 428, 717, 741
AluBI AGCT 1 cut(s) 244
AluI AGCT 1 cut(s) 244
Alw26I GTCTC 1 cut(s) 488
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 8 cut(s) 25, 56, 531, 566, 702, 720, 739, 744
ApeKI GCWGC 3 cut(s) 30, 33, 435
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 5 cut(s) 264, 435, 505, 514, 549
AspS9I GGNCC 4 cut(s) 57, 566, 702, 745
AsuC2I CCSGG 3 cut(s) 55, 580, 773
AsuHPI GGTGA 3 cut(s) 310, 616, 655
AvaI CYCGRG 1 cut(s) 272
BanI GGYRCC 1 cut(s) 496
BanII GRGCYC 1 cut(s) 53
BbvCI CCTCAGC 1 cut(s) 671
BbvI GCAGC 3 cut(s) 42, 45, 447
BccI CCATC 3 cut(s) 601, 625, 634
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 4 cut(s) 412, 430, 719, 743
BclI TGATCA 1 cut(s) 793
BcnI CCSGG 3 cut(s) 55, 580, 773
BcoDI GTCTC 1 cut(s) 488
BfuAI ACCTGC 1 cut(s) 460
Bme1390I CCNGG 7 cut(s) 55, 412, 430, 580, 719, 743, 773
BmeT110I CYCGRG 1 cut(s) 272
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 4 cut(s) 57, 566, 702, 745
BmiI GGNNCC 3 cut(s) 498, 567, 703
BmrFI CCNGG 7 cut(s) 55, 412, 430, 580, 719, 743, 773
BmrI ACTGGG 2 cut(s) 220, 571
BmsI GCATC 2 cut(s) 609, 649
BmuI ACTGGG 2 cut(s) 220, 571
Bpu10I CCTNAGC 1 cut(s) 671
BpuEI CTTGAG 1 cut(s) 80
BpuMI CCSGG 3 cut(s) 55, 580, 773
BsaHI GRCGYC 6 cut(s) 138, 174, 207, 234, 369, 576
BsaI GGTCTC 1 cut(s) 488
BsaJI CCNNGG 2 cut(s) 53, 410
BsaXI ACNNNNNCTCC 8 cut(s) 128, 158, 179, 209, 335, 359, 365, 389
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 429
Bse1I ACTGG 5 cut(s) 226, 263, 394, 566, 708
Bse3DI GCAATG 1 cut(s) 827
BseBI CCWGG 4 cut(s) 412, 430, 719, 743
BseDI CCNNGG 2 cut(s) 53, 410
BseGI GGATG 1 cut(s) 664
BseLI CCNNNNNNNGG 2 cut(s) 288, 429
BseMI GCAATG 1 cut(s) 827
BseMII CTCAG 5 cut(s) 499, 662, 681, 800, 826
BseNI ACTGG 5 cut(s) 226, 263, 394, 566, 708
BseRI GAGGAG 8 cut(s) 98, 133, 236, 317, 650, 688, 691, 798
BseX3I CGGCCG 1 cut(s) 25
BseXI GCAGC 3 cut(s) 42, 45, 447
BseYI CCCAGC 1 cut(s) 240
Bsh1236I CGCG 3 cut(s) 514, 536, 549
Bsh1285I CGRYCG 2 cut(s) 28, 406
BshFI GGCC 8 cut(s) 27, 58, 533, 568, 704, 722, 741, 746
BshNI GGYRCC 1 cut(s) 496
BsiEI CGRYCG 2 cut(s) 28, 406
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 3 cut(s) 55, 579, 773
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 2 cut(s) 288, 429
BsmAI GTCTC 1 cut(s) 488
BsmFI GGGAC 1 cut(s) 536
BsnI GGCC 8 cut(s) 27, 58, 533, 568, 704, 722, 741, 746
Bso31I GGTCTC 1 cut(s) 488
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 1 cut(s) 793
BspANI GGCC 8 cut(s) 27, 58, 533, 568, 704, 722, 741, 746
BspCNI CTCAG 5 cut(s) 498, 663, 682, 799, 825
BspFNI CGCG 3 cut(s) 514, 536, 549
BspLI GGNNCC 3 cut(s) 498, 567, 703
BspMI ACCTGC 1 cut(s) 460
BspQI GCTCTTC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 496
BspTNI GGTCTC 1 cut(s) 488
BsrBI CCGCTC 2 cut(s) 333, 725
BsrDI GCAATG 1 cut(s) 827
BsrI ACTGG 5 cut(s) 226, 263, 394, 566, 708
BssECI CCNNGG 2 cut(s) 53, 410
BssMI GATC 1 cut(s) 793
BssNI GRCGYC 6 cut(s) 138, 174, 207, 234, 369, 576
Bst2UI CCWGG 4 cut(s) 412, 430, 719, 743
Bst6I CTCTTC 4 cut(s) 41, 254, 320, 451
BstACI GRCGYC 6 cut(s) 138, 174, 207, 234, 369, 576
BstC8I GCNNGC 7 cut(s) 294, 501, 507, 516, 531, 624, 783
BstDEI CTNAG 5 cut(s) 485, 671, 690, 786, 812
BstF5I GGATG 1 cut(s) 664
BstFNI CGCG 3 cut(s) 514, 536, 549
BstHHI GCGC 5 cut(s) 264, 435, 505, 514, 549
BstKTI GATC 1 cut(s) 796
BstMAI GTCTC 1 cut(s) 488
BstMBI GATC 1 cut(s) 793
BstMCI CGRYCG 2 cut(s) 28, 406
BstMWI GCNNNNNNNGC 8 cut(s) 33, 496, 509, 511, 515, 599, 608, 623
BstNI CCWGG 4 cut(s) 412, 430, 719, 743
BstSCI CCNGG 7 cut(s) 53, 410, 428, 578, 717, 741, 771
BstUI CGCG 3 cut(s) 514, 536, 549
BstV1I GCAGC 3 cut(s) 42, 45, 447
BstZI CGGCCG 1 cut(s) 25
BsuRI GGCC 8 cut(s) 27, 58, 533, 568, 704, 722, 741, 746
BtgZI GCGATG 2 cut(s) 403, 632
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 1 cut(s) 664
BtsIMutI CAGTG 2 cut(s) 715, 803
BveI ACCTGC 1 cut(s) 460
Cac8I GCNNGC 7 cut(s) 294, 501, 507, 516, 531, 624, 783
CfoI GCGC 5 cut(s) 264, 435, 505, 514, 549
Cfr13I GGNCC 4 cut(s) 57, 566, 702, 745
CseI GACGC 5 cut(s) 146, 182, 215, 377, 584
CviAII CATG 1 cut(s) 493
DdeI CTNAG 5 cut(s) 485, 671, 690, 786, 812
DpnI GATC 1 cut(s) 795
DpnII GATC 1 cut(s) 793
DrdI GACNNNNNNGTC 1 cut(s) 386
DseDI GACNNNNNNGTC 1 cut(s) 386
EaeI YGGCCR 1 cut(s) 25
EagI CGGCCG 1 cut(s) 25
Eam1104I CTCTTC 4 cut(s) 41, 254, 320, 451
EarI CTCTTC 4 cut(s) 41, 254, 320, 451
EciI GGCGGA 5 cut(s) 110, 208, 337, 626, 641
EclXI CGGCCG 1 cut(s) 25
Eco147I AGGCCT 1 cut(s) 741
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 488
Eco52I CGGCCG 1 cut(s) 25
Eco88I CYCGRG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 566
EcoRII CCWGG 4 cut(s) 410, 428, 717, 741
EcoT38I GRGCYC 1 cut(s) 53
FaeI CATG 1 cut(s) 496
FaiI YATR 1 cut(s) 494
FaqI GGGAC 1 cut(s) 536
FatI CATG 1 cut(s) 492
FbaI TGATCA 1 cut(s) 793
FblI GTMKAC 1 cut(s) 171
FokI GGATG 1 cut(s) 671
FriOI GRGCYC 1 cut(s) 53
GlaI GCGC 5 cut(s) 263, 434, 504, 513, 548
GsaI CCCAGC 1 cut(s) 244
HaeIII GGCC 8 cut(s) 27, 58, 533, 568, 704, 722, 741, 746
HapII CCGG 3 cut(s) 55, 579, 773
HgaI GACGC 5 cut(s) 146, 182, 215, 377, 584
HhaI GCGC 5 cut(s) 264, 435, 505, 514, 549
Hin1I GRCGYC 6 cut(s) 138, 174, 207, 234, 369, 576
Hin1II CATG 1 cut(s) 496
Hin6I GCGC 5 cut(s) 262, 433, 503, 512, 547
HinP1I GCGC 5 cut(s) 262, 433, 503, 512, 547
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 5 cut(s) 215, 344, 362, 374, 805
HpaII CCGG 3 cut(s) 55, 579, 773
HphI GGTGA 3 cut(s) 310, 616, 655
Hpy166II GTNNAC 2 cut(s) 172, 715
Hpy188I TCNGA 7 cut(s) 79, 136, 232, 355, 367, 379, 767
Hpy188III TCNNGA 3 cut(s) 272, 274, 734
Hpy8I GTNNAC 2 cut(s) 172, 715
HpyAV CCTTC 7 cut(s) 70, 292, 346, 450, 580, 592, 731
HpyCH4IV ACGT 2 cut(s) 168, 234
HpyCH4V TGCA 2 cut(s) 622, 801
HpyF10VI GCNNNNNNNGC 8 cut(s) 33, 496, 509, 511, 515, 599, 608, 623
HpyF3I CTNAG 5 cut(s) 485, 671, 690, 786, 812
HpySE526I ACGT 2 cut(s) 168, 234
Hsp92I GRCGYC 6 cut(s) 138, 174, 207, 234, 369, 576
Hsp92II CATG 1 cut(s) 496
HspAI GCGC 5 cut(s) 262, 433, 503, 512, 547
Ksp22I TGATCA 1 cut(s) 793
Kzo9I GATC 1 cut(s) 793
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 3 cut(s) 249, 338, 544
Lsp1109I GCAGC 3 cut(s) 42, 45, 447
LweI GCATC 2 cut(s) 609, 649
MaeII ACGT 2 cut(s) 168, 234
MalI GATC 1 cut(s) 795
MbiI CCGCTC 2 cut(s) 333, 725
MboI GATC 1 cut(s) 793
MboII GAAGA 6 cut(s) 58, 241, 307, 371, 438, 595
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 2 cut(s) 749, 776
MlyI GAGTC 4 cut(s) 209, 338, 368, 799
MmeI TCCRAC 5 cut(s) 159, 255, 378, 390, 402
MroXI GAANNNNTTC 1 cut(s) 281
MspI CCGG 3 cut(s) 55, 579, 773
MspR9I CCNGG 7 cut(s) 55, 412, 430, 580, 719, 743, 773
MvaI CCWGG 4 cut(s) 412, 430, 719, 743
MvnI CGCG 3 cut(s) 514, 536, 549
MwoI GCNNNNNNNGC 8 cut(s) 33, 496, 509, 511, 515, 599, 608, 623
NciI CCSGG 3 cut(s) 55, 580, 773
NdeII GATC 1 cut(s) 793
NlaIII CATG 1 cut(s) 496
NlaIV GGNNCC 3 cut(s) 498, 567, 703
PaeR7I CTCGAG 1 cut(s) 272
PaqCI CACCTGC 1 cut(s) 460
PceI AGGCCT 1 cut(s) 741
PciSI GCTCTTC 1 cut(s) 41
PcsI WCGNNNNNNNCGW 4 cut(s) 210, 363, 375, 546
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 1 cut(s) 362
PflMI CCANNNNNTGG 1 cut(s) 429
PleI GAGTC 4 cut(s) 209, 338, 368, 799
PpsI GAGTC 4 cut(s) 209, 338, 368, 799
Psp6I CCWGG 4 cut(s) 410, 428, 717, 741
PspFI CCCAGC 1 cut(s) 240
PspGI CCWGG 4 cut(s) 410, 428, 717, 741
PspN4I GGNNCC 3 cut(s) 498, 567, 703
PspPI GGNCC 4 cut(s) 57, 566, 702, 745
SalI GTCGAC 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 41
Sau3AI GATC 1 cut(s) 793
Sau96I GGNCC 4 cut(s) 57, 566, 702, 745
SchI GAGTC 4 cut(s) 209, 338, 368, 799
ScrFI CCNGG 7 cut(s) 55, 412, 430, 580, 719, 743, 773
SduI GDGCHC 1 cut(s) 53
SfaNI GCATC 2 cut(s) 609, 649
Sfr274I CTCGAG 1 cut(s) 272
SgrDI CGTCGACG 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 2 cut(s) 95, 272
SmoI CTYRAG 2 cut(s) 95, 272
Sse9I AATT 2 cut(s) 749, 776
SseBI AGGCCT 1 cut(s) 741
StuI AGGCCT 1 cut(s) 741
StyD4I CCNGG 7 cut(s) 53, 410, 428, 578, 717, 741, 771
TaiI ACGT 2 cut(s) 171, 237
TaqI TCGA 5 cut(s) 171, 213, 273, 342, 465
TasI AATT 2 cut(s) 749, 776
TauI GCSGC 8 cut(s) 30, 224, 512, 536, 596, 707, 725, 828
TfiI GAWTC 1 cut(s) 362
TscAI CASTG 2 cut(s) 715, 803
TseI GCWGC 3 cut(s) 30, 33, 435
TspRI CASTG 2 cut(s) 715, 803
Van91I CCANNNNNTGG 1 cut(s) 429
XcmI CCANNNNNNNNNTGG 1 cut(s) 755
XhoI CTCGAG 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 281
ZraI GACGTC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.