RLG00000003606

Seed dormancy control

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0016591
Physical Location & Seq
Forward (+)
50634701 .. 50635671
971 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003606

Sequence Viewer

Length: 756 bp
ATGTTGAAAGCAGTAGGTCTGTTCGGGCGCAGCAGCAACAACAAGAAGAGCCCCCGGCCCTTCAAGGACTACTACGCCGAATGGCTGACAGCTCTTAAGTCCTCCCTCCTCCCTTCCCTCCGCCAATCCCTCTCCGACGCCTCCTCCCTCTCCCACCTCTCCCTCCACGTCGACGCCCTCCACCGCCACTTCCTCTCCTACTACGACGCCCTCGACTCCGCCGCCCAGTCCGACGTCGCCCAGCTCCTCTTCCCCGACTGGCGCAACCCTCTCGAGAAGCCCTTCCTCTGGCTCGCCGACCTCCACCCCTACCTCTTCACCAACCTCCTCCGCTCCTTCCTCGACTCCGCCTCCGACGAAGATTCCGACAGCCTCGTCGTCCAGTTCGGCGACCGCCCCTGGCACATCGCCACCGCCTGGCGCAGCCCTTCCTCTTCTCTCACGCAAAAGGTCGAGCAGGTGGAGTGCGGTCTCAGGCTCATGGTGCCTGCGCTGGCGGCGCGTGCGAGGGAGGCCCAGGCCGCGTTCGTGGAGCGCGTGGCGAGGAACTGGGGGCCTTACGACGCCGGGAAGAAGGAGGCGGCGAAGGCGGTGATGGCGGAGGCGATGGAGGCGGAGATGGAGGAGATGGCCTGGCCCAATTTCTCGTTGGGTTCAGAAACCCGGAATTGCTTGCTCAGTTTGATCAGTGCAAGACTCCTCTCAGTAAGCAATGCCGCTTGGCTGTTTGATTCTGGGGGATTGATGAGATTGTGA

Protein Analysis

252

Amino Acids

28.05

Weight (kDa)

6.08

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 37 - 112 2.5e-20 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016591)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22600
fragaria_vesca FvH4_5g00261
malus_domestica MD06G1063700.v1.1
prunus_persica Prupe.5G075300_v2.0.a1
pyrus_communis pycom06g05990
rosa_chinensis RchiOBHm_Chr7g0202371
rosa_laevigata RLG00000003606
rosa_multiflora Rmu_sc0000266.1_g000010
rosa_rugosa Rorug07G0070100
rosa_samantha Rh7AG197600 Rh7BG198600 Rh7CG208700 Rh7DG204400
rosa_wichuraiana Rw7G017320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 448
AasI GACNNNNNNGTC 1 cut(s) 374
AatII GACGTC 1 cut(s) 237
Acc36I ACCTGC 1 cut(s) 448
AccB1I GGYRCC 1 cut(s) 484
AccB7I CCANNNNNTGG 1 cut(s) 417
AccBSI CCGCTC 1 cut(s) 333
AccI GTMKAC 1 cut(s) 171
AccII CGCG 3 cut(s) 502, 524, 537
AcyI GRCGYC 5 cut(s) 138, 174, 207, 234, 564
AfiI CCNNNNNNNGG 2 cut(s) 288, 417
AflII CTTAAG 1 cut(s) 95
AgsI TTSAA 2 cut(s) 7, 64
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 4 cut(s) 398, 416, 516, 632
AluBI AGCT 2 cut(s) 92, 244
AluI AGCT 2 cut(s) 92, 244
Alw26I GTCTC 1 cut(s) 476
Ama87I CYCGRG 1 cut(s) 272
AoxI GGCC 6 cut(s) 56, 513, 519, 554, 630, 635
ApeKI GCWGC 3 cut(s) 30, 33, 423
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 6 cut(s) 30, 264, 423, 493, 502, 537
AspS9I GGNCC 4 cut(s) 57, 514, 554, 636
AsuC2I CCSGG 3 cut(s) 55, 568, 664
AsuHPI GGTGA 2 cut(s) 310, 604
AvaI CYCGRG 1 cut(s) 272
BanI GGYRCC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 53
BbvI GCAGC 3 cut(s) 42, 45, 435
BccI CCATC 4 cut(s) 589, 601, 613, 622
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 4 cut(s) 400, 418, 518, 634
BclI TGATCA 1 cut(s) 684
BcnI CCSGG 3 cut(s) 55, 568, 664
BcoDI GTCTC 1 cut(s) 476
BfrI CTTAAG 1 cut(s) 95
BfuAI ACCTGC 1 cut(s) 448
BisI GCNGC 8 cut(s) 31, 34, 222, 424, 498, 522, 582, 717
BlsI GCNGC 8 cut(s) 32, 35, 223, 425, 499, 523, 583, 718
Bme1390I CCNGG 7 cut(s) 55, 400, 418, 518, 568, 634, 664
BmeT110I CYCGRG 1 cut(s) 272
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 4 cut(s) 57, 514, 554, 636
BmiI GGNNCC 2 cut(s) 486, 555
BmrFI CCNGG 7 cut(s) 55, 400, 418, 518, 568, 634, 664
BmrI ACTGGG 2 cut(s) 220, 559
BmuI ACTGGG 2 cut(s) 220, 559
BpuMI CCSGG 3 cut(s) 55, 568, 664
BsaHI GRCGYC 5 cut(s) 138, 174, 207, 234, 564
BsaI GGTCTC 1 cut(s) 476
BsaJI CCNNGG 3 cut(s) 53, 398, 516
BsaXI ACNNNNNCTCC 6 cut(s) 128, 158, 179, 209, 335, 365
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 417
Bse1I ACTGG 4 cut(s) 226, 263, 382, 554
Bse3DI GCAATG 1 cut(s) 718
BseBI CCWGG 4 cut(s) 400, 418, 518, 634
BseDI CCNNGG 3 cut(s) 53, 398, 516
BseLI CCNNNNNNNGG 2 cut(s) 288, 417
BseMI GCAATG 1 cut(s) 718
BseMII CTCAG 3 cut(s) 487, 691, 717
BseNI ACTGG 4 cut(s) 226, 263, 382, 554
BseRI GAGGAG 6 cut(s) 98, 133, 236, 317, 638, 689
BseXI GCAGC 3 cut(s) 42, 45, 435
BseYI CCCAGC 1 cut(s) 240
Bsh1236I CGCG 3 cut(s) 502, 524, 537
Bsh1285I CGRYCG 1 cut(s) 394
BshFI GGCC 6 cut(s) 58, 515, 521, 556, 632, 637
BshNI GGYRCC 1 cut(s) 484
BsiEI CGRYCG 1 cut(s) 394
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 3 cut(s) 55, 567, 664
BslI CCNNNNNNNGG 2 cut(s) 288, 417
BsmAI GTCTC 1 cut(s) 476
BsnI GGCC 6 cut(s) 58, 515, 521, 556, 632, 637
Bso31I GGTCTC 1 cut(s) 476
BsoBI CYCGRG 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 1 cut(s) 684
BspANI GGCC 6 cut(s) 58, 515, 521, 556, 632, 637
BspCNI CTCAG 3 cut(s) 486, 690, 716
BspFNI CGCG 3 cut(s) 502, 524, 537
BspLI GGNNCC 2 cut(s) 486, 555
BspMI ACCTGC 1 cut(s) 448
BspQI GCTCTTC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 484
BspTI CTTAAG 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 476
BsrBI CCGCTC 1 cut(s) 333
BsrDI GCAATG 1 cut(s) 718
BsrI ACTGG 4 cut(s) 226, 263, 382, 554
BssECI CCNNGG 3 cut(s) 53, 398, 516
BssMI GATC 1 cut(s) 684
BssNI GRCGYC 5 cut(s) 138, 174, 207, 234, 564
Bst2UI CCWGG 4 cut(s) 400, 418, 518, 634
Bst6I CTCTTC 4 cut(s) 41, 254, 320, 439
BstACI GRCGYC 5 cut(s) 138, 174, 207, 234, 564
BstAFI CTTAAG 1 cut(s) 95
BstC8I GCNNGC 5 cut(s) 294, 489, 495, 504, 674
BstDEI CTNAG 3 cut(s) 473, 677, 703
BstFNI CGCG 3 cut(s) 502, 524, 537
BstHHI GCGC 6 cut(s) 30, 264, 423, 493, 502, 537
BstKTI GATC 1 cut(s) 687
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 1 cut(s) 684
BstMCI CGRYCG 1 cut(s) 394
BstMWI GCNNNNNNNGC 9 cut(s) 484, 497, 499, 503, 512, 521, 587, 596, 611
BstNI CCWGG 4 cut(s) 400, 418, 518, 634
BstSCI CCNGG 7 cut(s) 53, 398, 416, 516, 566, 632, 662
BstUI CGCG 3 cut(s) 502, 524, 537
BstV1I GCAGC 3 cut(s) 42, 45, 435
BsuRI GGCC 6 cut(s) 58, 515, 521, 556, 632, 637
BtgZI GCGATG 2 cut(s) 391, 620
BtrI CACGTC 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 694
BveI ACCTGC 1 cut(s) 448
Cac8I GCNNGC 5 cut(s) 294, 489, 495, 504, 674
CfoI GCGC 6 cut(s) 30, 264, 423, 493, 502, 537
Cfr13I GGNCC 4 cut(s) 57, 514, 554, 636
CseI GACGC 4 cut(s) 146, 182, 215, 572
CviAII CATG 1 cut(s) 481
DdeI CTNAG 3 cut(s) 473, 677, 703
DpnI GATC 1 cut(s) 686
DpnII GATC 1 cut(s) 684
DrdI GACNNNNNNGTC 1 cut(s) 374
DseDI GACNNNNNNGTC 1 cut(s) 374
Eam1104I CTCTTC 4 cut(s) 41, 254, 320, 439
EarI CTCTTC 4 cut(s) 41, 254, 320, 439
EciI GGCGGA 5 cut(s) 110, 208, 337, 614, 629
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 476
Eco88I CYCGRG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 554
EcoRII CCWGG 4 cut(s) 398, 416, 516, 632
EcoT38I GRGCYC 1 cut(s) 53
FaeI CATG 1 cut(s) 484
FaiI YATR 1 cut(s) 482
FatI CATG 1 cut(s) 480
FbaI TGATCA 1 cut(s) 684
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 8 cut(s) 31, 34, 222, 424, 498, 522, 582, 717
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 8 cut(s) 31, 34, 222, 424, 498, 522, 582, 717
GlaI GCGC 6 cut(s) 29, 263, 422, 492, 501, 536
GluI GCNGC 8 cut(s) 31, 34, 222, 424, 498, 522, 582, 717
GsaI CCCAGC 1 cut(s) 244
HaeIII GGCC 6 cut(s) 58, 515, 521, 556, 632, 637
HapII CCGG 3 cut(s) 55, 567, 664
HgaI GACGC 4 cut(s) 146, 182, 215, 572
HhaI GCGC 6 cut(s) 30, 264, 423, 493, 502, 537
Hin1I GRCGYC 5 cut(s) 138, 174, 207, 234, 564
Hin1II CATG 1 cut(s) 484
Hin6I GCGC 6 cut(s) 28, 262, 421, 491, 500, 535
HinP1I GCGC 6 cut(s) 28, 262, 421, 491, 500, 535
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 5 cut(s) 215, 344, 362, 696, 731
HpaII CCGG 3 cut(s) 55, 567, 664
HphI GGTGA 2 cut(s) 310, 604
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 5 cut(s) 136, 232, 355, 367, 658
Hpy188III TCNNGA 2 cut(s) 272, 274
Hpy8I GTNNAC 1 cut(s) 172
Hpy99I CGWCG 9 cut(s) 140, 173, 176, 209, 236, 239, 359, 380, 566
HpyAV CCTTC 7 cut(s) 70, 123, 292, 346, 438, 568, 580
HpyCH4IV ACGT 2 cut(s) 168, 234
HpyCH4V TGCA 1 cut(s) 692
HpyF10VI GCNNNNNNNGC 9 cut(s) 484, 497, 499, 503, 512, 521, 587, 596, 611
HpyF3I CTNAG 3 cut(s) 473, 677, 703
HpySE526I ACGT 2 cut(s) 168, 234
Hsp92I GRCGYC 5 cut(s) 138, 174, 207, 234, 564
Hsp92II CATG 1 cut(s) 484
HspAI GCGC 6 cut(s) 28, 262, 421, 491, 500, 535
Ksp22I TGATCA 1 cut(s) 684
Kzo9I GATC 1 cut(s) 684
LguI GCTCTTC 1 cut(s) 41
LmnI GCTCC 3 cut(s) 249, 338, 532
Lsp1109I GCAGC 3 cut(s) 42, 45, 435
MaeII ACGT 2 cut(s) 168, 234
MalI GATC 1 cut(s) 686
MbiI CCGCTC 1 cut(s) 333
MboI GATC 1 cut(s) 684
MboII GAAGA 6 cut(s) 58, 241, 307, 371, 426, 583
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 2 cut(s) 640, 667
MlyI GAGTC 3 cut(s) 209, 338, 690
MmeI TCCRAC 4 cut(s) 159, 255, 378, 390
MroXI GAANNNNTTC 1 cut(s) 281
MseI TTAA 1 cut(s) 96
MspCI CTTAAG 1 cut(s) 95
MspI CCGG 3 cut(s) 55, 567, 664
MspR9I CCNGG 7 cut(s) 55, 400, 418, 518, 568, 634, 664
MvaI CCWGG 4 cut(s) 400, 418, 518, 634
MvnI CGCG 3 cut(s) 502, 524, 537
MwoI GCNNNNNNNGC 9 cut(s) 484, 497, 499, 503, 512, 521, 587, 596, 611
NciI CCSGG 3 cut(s) 55, 568, 664
NdeII GATC 1 cut(s) 684
NlaIII CATG 1 cut(s) 484
NlaIV GGNNCC 2 cut(s) 486, 555
PaeR7I CTCGAG 1 cut(s) 272
PaqCI CACCTGC 1 cut(s) 448
PciSI GCTCTTC 1 cut(s) 41
PcsI WCGNNNNNNNCGW 3 cut(s) 210, 363, 534
PdmI GAANNNNTTC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 362, 731
PflMI CCANNNNNTGG 1 cut(s) 417
PkrI GCNGC 8 cut(s) 32, 35, 223, 425, 499, 523, 583, 718
PleI GAGTC 3 cut(s) 209, 338, 690
PpsI GAGTC 3 cut(s) 209, 338, 690
Psp6I CCWGG 4 cut(s) 398, 416, 516, 632
PspFI CCCAGC 1 cut(s) 240
PspGI CCWGG 4 cut(s) 398, 416, 516, 632
PspN4I GGNNCC 2 cut(s) 486, 555
PspPI GGNCC 4 cut(s) 57, 514, 554, 636
SalI GTCGAC 1 cut(s) 170
SapI GCTCTTC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 96
SatI GCNGC 8 cut(s) 31, 34, 222, 424, 498, 522, 582, 717
Sau3AI GATC 1 cut(s) 684
Sau96I GGNCC 4 cut(s) 57, 514, 554, 636
SchI GAGTC 3 cut(s) 209, 338, 690
ScrFI CCNGG 7 cut(s) 55, 400, 418, 518, 568, 634, 664
SduI GDGCHC 1 cut(s) 53
Sfr274I CTCGAG 1 cut(s) 272
SgrDI CGTCGACG 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 2 cut(s) 95, 272
SmoI CTYRAG 2 cut(s) 95, 272
Sse9I AATT 2 cut(s) 640, 667
StyD4I CCNGG 7 cut(s) 53, 398, 416, 516, 566, 632, 662
TaiI ACGT 2 cut(s) 171, 237
TaqI TCGA 5 cut(s) 171, 213, 273, 342, 453
TasI AATT 2 cut(s) 640, 667
TauI GCSGC 5 cut(s) 224, 500, 524, 584, 719
TfiI GAWTC 2 cut(s) 362, 731
Tru1I TTAA 1 cut(s) 96
Tru9I TTAA 1 cut(s) 96
TscAI CASTG 1 cut(s) 694
TseI GCWGC 3 cut(s) 30, 33, 423
TspRI CASTG 1 cut(s) 694
Van91I CCANNNNNTGG 1 cut(s) 417
Vha464I CTTAAG 1 cut(s) 95
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XhoI CTCGAG 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 281
ZraI GACGTC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.