FvH4_5g00261

Seed dormancy control

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0016591
Physical Location & Seq
Forward (+)
177044 .. 178499
1456 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g00261.t1

Sequence Viewer

Length: 831 bp
ATGCTGAAAGCCGTAGGATTGTTCGGCGGCCGGAACAAGAAGAGCCCGCCGCTCTTCAAGGACTACTACGCCGAGTGGATCACGACCCTGAAGAGCACCCTCCTCCCCTCCCTCCACCAATCCCTCTCCGACACGTCACCCTCCTCCCTCCCCCGCCTCTCCCTCCACGTCGACGCCCTCCACCGCCACTTCCAGTCCTACTACGACTCCCTCGACGCCGCCGCCCAAAACGACGTCGCTCAGATCCTCTTCCCCGACTGGCGCAACCCTCTCGAGAAGCCCTTCCTCTGGCTCGCCGACCTCCACCCCTACCTCTTCACCAACCTCCTCCGCTCCTTCCTCGACCAAGACTCCGACGACGACGACGACGACAGCCTCGTCGTCCAATTCGGCGACCGCCCCTGGCACGTCGCCACCGCCTGGCGCAGCCCTTCCAATTCTCTCGCGCAGAAGGTCGAACAGGTGGAGTGCGGCCTCAGGCTCATGGTGCCAGCGCTGGCGGCGCGTGCCAGGGACGCGCAGGCCGCCTTCGTGGAGCGCGTGGCCAAGAATTGGGGGCCCTTCGACGGCGGGAAGAAGGAGACGGCGAAGGCGGTGATGGCGGAGGCGATGGAGGCGGAGATGGAGGAGATGGTGAGCGTTGTGCTGGATGCGAATAGGCTGAGGAGGAGTGTTCTTGCCGAGATCATCGGGGCCACCAGTGTTTACCAGGCCGCTCTGTTTCTTGAAGGCCTGGCCCAGTTTCTTGTTGGGTTCAGAAACCCGGAGTTGCTGGCCCAGTTTGATCAGTGCAAGACTCCTCTCACTAAGCAATGCCGCTTGGCAGTTTGA

Protein Analysis

277

Amino Acids

30.94

Weight (kDa)

5.55

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DOG1 PF14144 35 - 112 1.1e-19 Seed dormancy control
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016591)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22600
fragaria_vesca FvH4_5g00261
malus_domestica MD06G1063700.v1.1
prunus_persica Prupe.5G075300_v2.0.a1
pyrus_communis pycom06g05990
rosa_chinensis RchiOBHm_Chr7g0202371
rosa_laevigata RLG00000003606
rosa_multiflora Rmu_sc0000266.1_g000010
rosa_rugosa Rorug07G0070100
rosa_samantha Rh7AG197600 Rh7BG198600 Rh7CG208700 Rh7DG204400
rosa_wichuraiana Rw7G017320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 377
AatII GACGTC 1 cut(s) 237
AccB1I GGYRCC 1 cut(s) 487
AccB7I CCANNNNNTGG 2 cut(s) 420, 552
AccBSI CCGCTC 3 cut(s) 52, 333, 716
AccI GTMKAC 1 cut(s) 171
AccII CGCG 4 cut(s) 446, 505, 518, 540
AclWI GGATC 2 cut(s) 86, 238
AcoI YGGCCR 2 cut(s) 28, 543
AcuI CTGAAG 1 cut(s) 110
AcyI GRCGYC 3 cut(s) 174, 216, 234
AfeI AGCGCT 1 cut(s) 495
AfiI CCNNNNNNNGG 4 cut(s) 288, 420, 552, 566
AflIII ACRYGT 1 cut(s) 132
AgsI TTSAA 2 cut(s) 58, 728
AjiI CACGTC 3 cut(s) 135, 169, 409
AjnI CCWGG 5 cut(s) 401, 419, 509, 708, 732
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 575
AlwI GGATC 2 cut(s) 86, 238
Ama87I CYCGRG 1 cut(s) 272
Aor51HI AGCGCT 1 cut(s) 495
ApaI GGGCCC 1 cut(s) 561
ApeKI GCWGC 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 281
AspLEI GCGC 7 cut(s) 264, 426, 448, 496, 505, 520, 540
AspS9I GGNCC 5 cut(s) 557, 558, 693, 736, 775
AsuC2I CCSGG 1 cut(s) 764
AsuHPI GGTGA 4 cut(s) 129, 310, 607, 646
AvaI CYCGRG 1 cut(s) 272
AxyI CCTNAGG 1 cut(s) 476
BaeGI GKGCMC 1 cut(s) 561
BalI TGGCCA 1 cut(s) 545
BanI GGYRCC 1 cut(s) 487
BanII GRGCYC 2 cut(s) 47, 561
Bbv12I GWGCWC 1 cut(s) 98
BbvCI CCTCAGC 1 cut(s) 662
BbvI GCAGC 1 cut(s) 438
BccI CCATC 4 cut(s) 592, 604, 616, 625
BceAI ACGGC 2 cut(s) 583, 600
BcgI CGANNNNNNTGC 2 cut(s) 253, 287
BciT130I CCWGG 5 cut(s) 403, 421, 511, 710, 734
BclI TGATCA 1 cut(s) 784
BcnI CCSGG 1 cut(s) 764
BcoDI GTCTC 1 cut(s) 575
BfoI RGCGCY 1 cut(s) 497
Bme1390I CCNGG 6 cut(s) 403, 421, 511, 710, 734, 764
BmeT110I CYCGRG 1 cut(s) 272
BmgBI CACGTC 3 cut(s) 135, 169, 409
BmgT120I GGNCC 5 cut(s) 557, 558, 693, 736, 775
BmiI GGNNCC 4 cut(s) 489, 558, 559, 694
BmrFI CCNGG 6 cut(s) 403, 421, 511, 710, 734, 764
BmrI ACTGGG 2 cut(s) 733, 772
BmsI GCATC 1 cut(s) 640
BmuI ACTGGG 2 cut(s) 733, 772
Bpu10I CCTNAGC 1 cut(s) 662
BpuMI CCSGG 1 cut(s) 764
BsaHI GRCGYC 3 cut(s) 174, 216, 234
BsaJI CCNNGG 2 cut(s) 401, 510
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 335, 365
Bsc4I CCNNNNNNNGG 4 cut(s) 288, 420, 552, 566
Bse1I ACTGG 5 cut(s) 193, 263, 699, 739, 778
Bse21I CCTNAGG 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 818
BseBI CCWGG 5 cut(s) 403, 421, 511, 710, 734
BseDI CCNNGG 2 cut(s) 401, 510
BseGI GGATG 1 cut(s) 655
BseLI CCNNNNNNNGG 4 cut(s) 288, 420, 552, 566
BseMI GCAATG 1 cut(s) 818
BseMII CTCAG 3 cut(s) 254, 490, 653
BseNI ACTGG 5 cut(s) 193, 263, 699, 739, 778
BseRI GAGGAG 7 cut(s) 92, 133, 317, 641, 679, 682, 789
BseSI GKGCMC 1 cut(s) 561
BseX3I CGGCCG 1 cut(s) 28
BseXI GCAGC 1 cut(s) 438
Bsh1236I CGCG 4 cut(s) 446, 505, 518, 540
Bsh1285I CGRYCG 2 cut(s) 31, 397
BshNI GGYRCC 1 cut(s) 487
BsiEI CGRYCG 2 cut(s) 31, 397
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 1 cut(s) 272
BsiSI CCGG 2 cut(s) 31, 764
BslFI GGGAC 1 cut(s) 527
BslI CCNNNNNNNGG 4 cut(s) 288, 420, 552, 566
BsmAI GTCTC 1 cut(s) 575
BsmBI CGTCTC 1 cut(s) 575
BsmFI GGGAC 1 cut(s) 527
BsoBI CYCGRG 1 cut(s) 272
Bsp120I GGGCCC 1 cut(s) 557
Bsp1286I GDGCHC 3 cut(s) 47, 98, 561
Bsp143I GATC 4 cut(s) 78, 243, 684, 784
BspCNI CTCAG 3 cut(s) 253, 489, 654
BspFNI CGCG 4 cut(s) 446, 505, 518, 540
BspLI GGNNCC 4 cut(s) 489, 558, 559, 694
BspPI GGATC 2 cut(s) 86, 238
BspQI GCTCTTC 3 cut(s) 35, 59, 86
BspT107I GGYRCC 1 cut(s) 487
BsrBI CCGCTC 3 cut(s) 52, 333, 716
BsrDI GCAATG 1 cut(s) 818
BsrI ACTGG 5 cut(s) 193, 263, 699, 739, 778
BssECI CCNNGG 2 cut(s) 401, 510
BssMI GATC 4 cut(s) 78, 243, 684, 784
BssNI GRCGYC 3 cut(s) 174, 216, 234
Bst2UI CCWGG 5 cut(s) 403, 421, 511, 710, 734
Bst6I CTCTTC 5 cut(s) 35, 59, 86, 254, 320
BstACI GRCGYC 3 cut(s) 174, 216, 234
BstC8I GCNNGC 7 cut(s) 47, 294, 492, 498, 507, 522, 774
BstDEI CTNAG 4 cut(s) 240, 476, 662, 807
BstF5I GGATG 1 cut(s) 655
BstFNI CGCG 4 cut(s) 446, 505, 518, 540
BstH2I RGCGCY 1 cut(s) 497
BstHHI GCGC 7 cut(s) 264, 426, 448, 496, 505, 520, 540
BstKTI GATC 4 cut(s) 81, 246, 687, 787
BstMAI GTCTC 1 cut(s) 575
BstMBI GATC 4 cut(s) 78, 243, 684, 784
BstMCI CGRYCG 2 cut(s) 31, 397
BstMWI GCNNNNNNNGC 8 cut(s) 487, 500, 502, 506, 515, 524, 599, 614
BstNI CCWGG 5 cut(s) 403, 421, 511, 710, 734
BstSCI CCNGG 6 cut(s) 401, 419, 509, 708, 732, 762
BstSLI GKGCMC 1 cut(s) 561
BstUI CGCG 4 cut(s) 446, 505, 518, 540
BstV1I GCAGC 1 cut(s) 438
BstX2I RGATCY 1 cut(s) 243
BstYI RGATCY 1 cut(s) 243
BstZI CGGCCG 1 cut(s) 28
Bsu36I CCTNAGG 1 cut(s) 476
BtgZI GCGATG 1 cut(s) 623
BtrI CACGTC 3 cut(s) 135, 169, 409
BtsCI GGATG 1 cut(s) 655
BtsIMutI CAGTG 2 cut(s) 706, 794
Cac8I GCNNGC 7 cut(s) 47, 294, 492, 498, 507, 522, 774
CfoI GCGC 7 cut(s) 264, 426, 448, 496, 505, 520, 540
Cfr13I GGNCC 5 cut(s) 557, 558, 693, 736, 775
CseI GACGC 3 cut(s) 182, 224, 524
CviAII CATG 1 cut(s) 484
DdeI CTNAG 4 cut(s) 240, 476, 662, 807
DpnI GATC 4 cut(s) 80, 245, 686, 786
DpnII GATC 4 cut(s) 78, 243, 684, 784
DrdI GACNNNNNNGTC 1 cut(s) 377
DseDI GACNNNNNNGTC 1 cut(s) 377
EaeI YGGCCR 2 cut(s) 28, 543
EagI CGGCCG 1 cut(s) 28
Eam1104I CTCTTC 5 cut(s) 35, 59, 86, 254, 320
EarI CTCTTC 5 cut(s) 35, 59, 86, 254, 320
EciI GGCGGA 2 cut(s) 617, 632
EclXI CGGCCG 1 cut(s) 28
Eco147I AGGCCT 1 cut(s) 732
Eco24I GRGCYC 2 cut(s) 47, 561
Eco47III AGCGCT 1 cut(s) 495
Eco52I CGGCCG 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 110
Eco81I CCTNAGG 1 cut(s) 476
Eco88I CYCGRG 1 cut(s) 272
EcoO109I RGGNCCY 2 cut(s) 557, 558
EcoRII CCWGG 5 cut(s) 401, 419, 509, 708, 732
EcoT38I GRGCYC 2 cut(s) 47, 561
Esp3I CGTCTC 1 cut(s) 575
FaeI CATG 1 cut(s) 487
FaiI YATR 1 cut(s) 485
FaqI GGGAC 1 cut(s) 527
FatI CATG 1 cut(s) 483
FauI CCCGC 3 cut(s) 54, 161, 563
FbaI TGATCA 1 cut(s) 784
FblI GTMKAC 1 cut(s) 171
FokI GGATG 1 cut(s) 662
FriOI GRGCYC 2 cut(s) 47, 561
GlaI GCGC 7 cut(s) 263, 425, 447, 495, 504, 519, 539
HaeII RGCGCY 1 cut(s) 497
HapII CCGG 2 cut(s) 31, 764
HgaI GACGC 3 cut(s) 182, 224, 524
HhaI GCGC 7 cut(s) 264, 426, 448, 496, 505, 520, 540
Hin1I GRCGYC 3 cut(s) 174, 216, 234
Hin1II CATG 1 cut(s) 487
Hin6I GCGC 7 cut(s) 262, 424, 446, 494, 503, 518, 538
HinP1I GCGC 7 cut(s) 262, 424, 446, 494, 503, 518, 538
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 3 cut(s) 206, 350, 796
HpaII CCGG 2 cut(s) 31, 764
HphI GGTGA 4 cut(s) 129, 310, 607, 646
Hpy166II GTNNAC 2 cut(s) 172, 706
Hpy188I TCNGA 4 cut(s) 130, 243, 355, 758
Hpy188III TCNNGA 4 cut(s) 82, 272, 274, 725
Hpy8I GTNNAC 2 cut(s) 172, 706
HpyAV CCTTC 9 cut(s) 292, 346, 441, 445, 538, 571, 571, 583, 722
HpyCH4IV ACGT 4 cut(s) 134, 168, 234, 408
HpyCH4V TGCA 1 cut(s) 792
HpyF10VI GCNNNNNNNGC 8 cut(s) 487, 500, 502, 506, 515, 524, 599, 614
HpyF3I CTNAG 4 cut(s) 240, 476, 662, 807
HpySE526I ACGT 4 cut(s) 134, 168, 234, 408
Hsp92I GRCGYC 3 cut(s) 174, 216, 234
Hsp92II CATG 1 cut(s) 487
HspAI GCGC 7 cut(s) 262, 424, 446, 494, 503, 518, 538
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 4 cut(s) 78, 243, 684, 784
LguI GCTCTTC 3 cut(s) 35, 59, 86
LmnI GCTCC 2 cut(s) 338, 535
Lsp1109I GCAGC 1 cut(s) 438
LweI GCATC 1 cut(s) 640
MaeII ACGT 4 cut(s) 134, 168, 234, 408
MaeIII GTNAC 1 cut(s) 135
MalI GATC 4 cut(s) 80, 245, 686, 786
MbiI CCGCTC 3 cut(s) 52, 333, 716
MboI GATC 4 cut(s) 78, 243, 684, 784
MboII GAAGA 6 cut(s) 46, 52, 103, 241, 307, 586
MflI RGATCY 1 cut(s) 243
MhlI GDGCHC 3 cut(s) 47, 98, 561
MlsI TGGCCA 1 cut(s) 545
MluCI AATT 3 cut(s) 386, 436, 550
MluNI TGGCCA 1 cut(s) 545
MlyI GAGTC 3 cut(s) 200, 344, 790
MmeI TCCRAC 2 cut(s) 153, 378
Mox20I TGGCCA 1 cut(s) 545
MroXI GAANNNNTTC 1 cut(s) 281
MscI TGGCCA 1 cut(s) 545
Msp20I TGGCCA 1 cut(s) 545
MspI CCGG 2 cut(s) 31, 764
MspR9I CCNGG 6 cut(s) 403, 421, 511, 710, 734, 764
MvaI CCWGG 5 cut(s) 403, 421, 511, 710, 734
MvnI CGCG 4 cut(s) 446, 505, 518, 540
MwoI GCNNNNNNNGC 8 cut(s) 487, 500, 502, 506, 515, 524, 599, 614
NciI CCSGG 1 cut(s) 764
NdeII GATC 4 cut(s) 78, 243, 684, 784
NlaIII CATG 1 cut(s) 487
NlaIV GGNNCC 4 cut(s) 489, 558, 559, 694
NmeAIII GCCGAG 2 cut(s) 97, 706
NmuCI GTSAC 1 cut(s) 135
PaeR7I CTCGAG 1 cut(s) 272
PceI AGGCCT 1 cut(s) 732
PciSI GCTCTTC 3 cut(s) 35, 59, 86
PcsI WCGNNNNNNNCGW 5 cut(s) 210, 363, 366, 375, 537
PdmI GAANNNNTTC 1 cut(s) 281
PflMI CCANNNNNTGG 2 cut(s) 420, 552
PleI GAGTC 3 cut(s) 200, 344, 790
PpsI GAGTC 3 cut(s) 200, 344, 790
Psp6I CCWGG 5 cut(s) 401, 419, 509, 708, 732
PspGI CCWGG 5 cut(s) 401, 419, 509, 708, 732
PspN4I GGNNCC 4 cut(s) 489, 558, 559, 694
PspOMI GGGCCC 1 cut(s) 557
PspPI GGNCC 5 cut(s) 557, 558, 693, 736, 775
PsuI RGATCY 1 cut(s) 243
SalI GTCGAC 1 cut(s) 170
SapI GCTCTTC 3 cut(s) 35, 59, 86
Sau3AI GATC 4 cut(s) 78, 243, 684, 784
Sau96I GGNCC 5 cut(s) 557, 558, 693, 736, 775
SchI GAGTC 3 cut(s) 200, 344, 790
ScrFI CCNGG 6 cut(s) 403, 421, 511, 710, 734, 764
SduI GDGCHC 3 cut(s) 47, 98, 561
SetI ASST 9 cut(s) 137, 171, 237, 303, 315, 327, 411, 456, 465
SfaNI GCATC 1 cut(s) 640
Sfr274I CTCGAG 1 cut(s) 272
SgrDI CGTCGACG 1 cut(s) 170
SlaI CTCGAG 1 cut(s) 272
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 3 cut(s) 386, 436, 550
SseBI AGGCCT 1 cut(s) 732
StuI AGGCCT 1 cut(s) 732
StyD4I CCNGG 6 cut(s) 401, 419, 509, 708, 732, 762
TaiI ACGT 4 cut(s) 137, 171, 237, 411
TaqI TCGA 6 cut(s) 171, 213, 273, 342, 456, 564
TasI AATT 3 cut(s) 386, 436, 550
TauI GCSGC 9 cut(s) 30, 52, 221, 224, 474, 503, 527, 716, 819
TscAI CASTG 2 cut(s) 706, 794
TseFI GTSAC 1 cut(s) 135
TseI GCWGC 1 cut(s) 426
Tsp45I GTSAC 1 cut(s) 135
TspRI CASTG 2 cut(s) 706, 794
Van91I CCANNNNNTGG 2 cut(s) 420, 552
XcmI CCANNNNNNNNNTGG 1 cut(s) 746
XhoI CTCGAG 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 281
ZraI GACGTC 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.