Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010732

Physical Location & Seq

Genomic Coordinates Reverse (-)

12962965 .. 12966629

Gene Structure 3665 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G25350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2238 bp

ATGAGGTTTGGGAAAGAGTTTGTGTCTCAGATGATCCCAGAATGGCAAGAAGCTTATATAGATTACGCTTACCTCAAAACCATTCTTCAAGACATCCAAGCTTCCCGGAATAGGTCAGATTCCAATAACCAGAGCAGTACTCCTTCCTTCGCACGTAACCTAACCAGACGGTACAACCGCGACGCCTTGGTCTCTGAGAATCACGACATTGTGGTCAACACTGTGACACGGCTCGAAGAAGGGCTCGAGACGGCGGCTTACGAGACTACGTTTTTAAAGGCCGGAGAAGCAGGTGGCGACTTTGAGGTTACCTTTTTTAGAACCCTAGATCGAGAATTCAACAAGGTAAATAACTTTTACAGGCTGAAAGTGGAGACAGCTAGAACTGAGGCTCTGGCTCTGAACAAACAAATGGATGCTTTGATTGCTTTCAGACACAAAGTCATGGATCAGAATCAGAAGAACCCTTCTGTCTTTGATTCAGTTTCAGAAGACATCAATGGCTCAGCTTCTGAGGTTGGATCATCTTCAAAGTGTACAGAACACAATGTGGCGTTAGCTGATCTTATGAGGAACGAAGATACATCGAATGAGAGTATTCTTGAGCGCATCAGGATGAACAAAACTCGAGAAATAACTCCTCTGTCCGCAATTAAAACTATACTCAAGGTCCATAAGCAGGACGAGCTCAAATTCACAAGAGATAATCTCAAGGAAGTGGAAAAGCGACTTCAGGTAGCGTTCATTGAGTTTTATCAGAAGCTTCGACATCTTAAAAACTACAGCTTCTTGAACGCTTCCGCTGTTTCCAAGATCATGAAGAAGTATGATAAGATAGCCAAAAGAAATGCTGCGAAACTGTATATGGAGATGGTCGATAAATCTTTCCTCAGTAGCTCCGAAGAAGTTCATAAGTTGTTGCTAAAGGTTGAATCTATTTTCATTGAACATTTCTCCAATTCAAATCGGAGGGAAGGGATGAGCCATTTAAGACCAAAAATAAATAAAGAACGTCATCTTATAACGTTTTCCACCGGCTTCTTTTTCGGCTGTGGTATTTCTCTCATTGTAGCTCTGGGTTTGATCATCCATGCTCGTAATATCATGGGTACACCGGGACAAAGAACTTATATGGAAACAATGTTCCCTCTTTACAGGTTTTTTGGGTTTGTAGTTTTGCACATGGACGTGTACGCTGCTAATATCTACTTCTGGAGGCGATACCGTGTGAATTACTCCTTCATCTTTGGATTCAAACAAGGCACTGAACTTGGTTATAGACATGTTCTACTTCTCAGCTTTGGTCTTGGTACACTTTCTTTATGTGCTGTCTTATTAAACCTTGACATGGAAATGGATGCTCAAACAAAAGATTACAGATTAGTCACCGAACTTATCCCCCTCTTTCTTCTCGTATTAGTGATTATCATTGTTCTCTGCCCCTTTAACATTTTGTATCGGTCAAGCCGATTCTTCTTCCTCTCGGTTCTGTTCCGCTGCATTGCTGCACCATTTTACGCGGTGCATCTTCCGGACTTTTTCTTGGGAGATCAACTAACAAGCCAGGTTCAAGCACTGAGGAGTCTAGAGTTCTACATATGCTACTATGGTTTTGGAGATTTCAGATATAGACGACGAAACACCTGCACATCAAATATTGGATTCAGAACATTCTACTTCATCGTCGCCGTTATACCTTACTGGTTACGGTTTCTTCAGTGCATCCGAAGAATGGTGGAAGACAGAGATCTGAGTCATGGCTACAATGGCATCAAATACCTTTTGACCATCGTTGCTGCTTCTCTTAGAACAGCTTATACTCTAAACCGAGGAAGCAACTGGAATATCACAGCTTGGGTTTTCTCAGGAGTTGCAACGTTTTACGGAACTTACTGGGACATTGTTCTTGACTGGGGATTGCTTCAAAGAGGATGTAAGAATAGTTTCTTGAGAGACAAGCTTCTTGTTCCTCACAAAACCGTGTACTACGCTGCAATGGTCCTGAATGTTTTGTTGAGGTTAGTGTGGTTGCAAACAGTTTTGGATCTGAAATTCTCTTTCTTGCATAGAGAAACTATGGTCGCGCTTATGGCTTGCCTTGAGATCATACGCAGAGGTATCTGGAACTTCTTTAGGCTGGAGAATGAGCATTTGAACAACGTTGGGAGATACAGGGCGTTCAAAACTGTCCCGTTACCGTTCAACTACGAAGAAGATGGAGATCATCATAACAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005794 GO:0005802 GO:0005829 GO:0005886 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008361 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0009791 GO:0009888 GO:0009960 GO:0009987 GO:0010017 GO:0010154 GO:0010468 GO:0012505 GO:0016020 GO:0016043 GO:0019222 GO:0022414 GO:0023052 GO:0031984 GO:0032501 GO:0032502 GO:0032535 GO:0040008 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045793 GO:0048316 GO:0048580 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051239 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0065008 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071840 GO:0071944 GO:0080050 GO:0080113 GO:0090066 GO:0098791 GO:0104004 GO:2000026 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EXS SPX

Protein Analysis

745

Amino Acids

86.98

Weight (kDa)

9.27

Isoelectric Point (pI)

43.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	1 - 292	1.4e-63	SPX domain
EXS	PF03124	381 - 718	5.9e-102	EXS family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010732)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G29060 AT4G25350
fragaria_vesca	FvH4_6g48060 FvH4_6g48060
malus_domestica	MD09G1054400.v1.1 MD17G1052300.v1.1
prunus_persica	Prupe.3G264100_v2.0.a1
pyrus_communis	pycom111g04570
rosa_chinensis	RchiOBHm_Chr2g0167381
rosa_laevigata	RLG00000021712 RLG00000021728
rosa_multiflora	Rmu_sc0001840.1_g000006
rosa_roxburghii	Rroxscaffold_2G00083560
rosa_rugosa	Rorug02G0529900.1 Rorug02G0530000
rosa_samantha	Rh2AG596800 Rh2BG608300 Rh2CG579100 Rh2DG619600
rosa_wichuraiana	Rw2G049680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1022
AarI	CACCTGC	2 cut(s)	281, 1652
AasI	GACNNNNNNGTC	2 cut(s)	188, 212
Acc36I	ACCTGC	2 cut(s)	281, 1652
AccII	CGCG	3 cut(s)	180, 1520, 2084
AccIII	TCCGGA	1 cut(s)	1531
AciI	CCGC	6 cut(s)	178, 254, 648, 801, 1495, 1520
AclI	AACGTT	3 cut(s)	1025, 1877, 2160
AclWI	GGATC	4 cut(s)	28, 456, 529, 2052
AcsI	RAATTY	3 cut(s)	335, 692, 2051
AcuI	CTGAAG	2 cut(s)	716, 1700
AcyI	GRCGYC	1 cut(s)	183
AdeI	CACNNNGTG	1 cut(s)	550
AfaI	GTAC	7 cut(s)	139, 173, 538, 1111, 1193, 1312, 1985
AfiI	CCNNNNNNNGG	4 cut(s)	111, 679, 1348, 1732
AflIII	ACRYGT	2 cut(s)	1188, 1282
AhdI	GACNNNNNGTC	1 cut(s)	440
AjiI	CACGTC	1 cut(s)	1189
AjnI	CCWGG	1 cut(s)	1563
AloI	GAACNNNNNNTCC	2 cut(s)	1127, 1159
Alw21I	GWGCWC	1 cut(s)	690
Alw26I	GTCTC	6 cut(s)	30, 196, 242, 257, 368, 1947
AlwI	GGATC	4 cut(s)	28, 456, 529, 2052
AlwNI	CAGNNNCTG	1 cut(s)	512
Ama87I	CYCGRG	2 cut(s)	245, 627
Aor13HI	TCCGGA	1 cut(s)	1531
AoxI	GGCC	1 cut(s)	279
ApeKI	GCWGC	6 cut(s)	851, 1196, 1497, 1505, 1796, 1991
ApoI	RAATTY	3 cut(s)	335, 692, 2051
Asp700I	GAANNNNTTC	2 cut(s)	906, 1943
AspLEI	GCGC	2 cut(s)	609, 2086
AspS9I	GGNCC	2 cut(s)	670, 1999
AsuC2I	CCSGG	2 cut(s)	106, 1116
AsuHPI	GGTGA	1 cut(s)	1378
AvaI	CYCGRG	2 cut(s)	245, 627
AvaII	GGWCC	2 cut(s)	670, 1999
BanII	GRGCYC	2 cut(s)	246, 690
BbsI	GAAGAC	2 cut(s)	498, 1746
Bbv12I	GWGCWC	1 cut(s)	690
BbvI	GCAGC	6 cut(s)	838, 1183, 1484, 1492, 1783, 1978
BccI	CCATC	3 cut(s)	865, 1796, 2210
BceAI	ACGGC	3 cut(s)	245, 267, 1673
BcgI	CGANNNNNNTGC	2 cut(s)	1626, 1660
BciT130I	CCWGG	1 cut(s)	1565
BclI	TGATCA	1 cut(s)	1083
BcnI	CCSGG	2 cut(s)	106, 1116
BcoDI	GTCTC	6 cut(s)	30, 196, 242, 257, 368, 1947
BfaI	CTAG	3 cut(s)	326, 381, 1586
BfmI	CTRYAG	1 cut(s)	781
BfuAI	ACCTGC	2 cut(s)	281, 1652
BglII	AGATCT	1 cut(s)	1747
BisI	GCNGC	7 cut(s)	255, 852, 1197, 1498, 1506, 1797, 1992
BlpI	GCTNAGC	1 cut(s)	505
BlsI	GCNGC	7 cut(s)	256, 853, 1198, 1499, 1507, 1798, 1993
BmcAI	AGTACT	1 cut(s)	139
Bme1390I	CCNGG	3 cut(s)	106, 1116, 1565
Bme18I	GGWCC	2 cut(s)	670, 1999
BmeRI	GACNNNNNGTC	1 cut(s)	440
BmeT110I	CYCGRG	2 cut(s)	245, 627
BmgBI	CACGTC	1 cut(s)	1189
BmgT120I	GGNCC	2 cut(s)	670, 1999
BmrFI	CCNGG	3 cut(s)	106, 1116, 1565
BmrI	ACTGGG	2 cut(s)	1903, 1921
BmsI	GCATC	6 cut(s)	406, 618, 1348, 1534, 1731, 1779
BmuI	ACTGGG	2 cut(s)	1903, 1921
BpiI	GAAGAC	2 cut(s)	498, 1746
BplI	GAGNNNNNCTC	4 cut(s)	124, 156, 693, 725
BpmI	CTGGAG	2 cut(s)	1234, 2159
Bpu1102I	GCTNAGC	1 cut(s)	505
BpuEI	CTTGAG	5 cut(s)	623, 650, 695, 1969, 2120
BpuMI	CCSGG	2 cut(s)	106, 1116
BsaAI	YACGTR	1 cut(s)	155
BsaBI	GATNNNNATC	2 cut(s)	453, 2220
BsaHI	GRCGYC	1 cut(s)	183
BsaI	GGTCTC	1 cut(s)	196
BsaJI	CCNNGG	2 cut(s)	186, 1828
BsaWI	WCCGGW	1 cut(s)	1531
Bsc4I	CCNNNNNNNGG	4 cut(s)	111, 679, 1348, 1732
Bse118I	RCCGGY	1 cut(s)	1034
Bse1I	ACTGG	4 cut(s)	1706, 1844, 1898, 1916
Bse3DI	GCAATG	2 cut(s)	1500, 2001
Bse8I	GATNNNNATC	2 cut(s)	453, 2220
BseAI	TCCGGA	1 cut(s)	1531
BseBI	CCWGG	1 cut(s)	1565
BseDI	CCNNGG	2 cut(s)	186, 1828
BseGI	GGATG	8 cut(s)	93, 421, 621, 984, 1086, 1363, 1722, 1937
BseJI	GATNNNNATC	2 cut(s)	453, 2220
BseLI	CCNNNNNNNGG	4 cut(s)	111, 679, 1348, 1732
BseMI	GCAATG	2 cut(s)	1500, 2001
BseNI	ACTGG	4 cut(s)	1706, 1844, 1898, 1916
BseRI	GAGGAG	2 cut(s)	630, 1594
BseXI	GCAGC	6 cut(s)	838, 1183, 1484, 1492, 1783, 1978
BsgI	GTGCAG	2 cut(s)	1491, 1630
Bsh1236I	CGCG	3 cut(s)	180, 1520, 2084
BshFI	GGCC	1 cut(s)	281
BsiHKAI	GWGCWC	1 cut(s)	690
BsiHKCI	CYCGRG	2 cut(s)	245, 627
BsiSI	CCGG	5 cut(s)	106, 282, 1035, 1115, 1532
BslFI	GGGAC	3 cut(s)	1131, 1910, 2174
BslI	CCNNNNNNNGG	4 cut(s)	111, 679, 1348, 1732
BsmAI	GTCTC	6 cut(s)	30, 196, 242, 257, 368, 1947
BsmBI	CGTCTC	1 cut(s)	242
BsmFI	GGGAC	3 cut(s)	1131, 1910, 2174
BsnI	GGCC	1 cut(s)	281
Bso31I	GGTCTC	1 cut(s)	196
BsoBI	CYCGRG	2 cut(s)	245, 627
Bsp1286I	GDGCHC	2 cut(s)	246, 690
Bsp13I	TCCGGA	1 cut(s)	1531
Bsp1407I	TGTACA	1 cut(s)	536
Bsp1720I	GCTNAGC	1 cut(s)	505
BspACI	CCGC	6 cut(s)	178, 254, 648, 801, 1495, 1520
BspANI	GGCC	1 cut(s)	281
BspEI	TCCGGA	1 cut(s)	1531
BspFNI	CGCG	3 cut(s)	180, 1520, 2084
BspHI	TCATGA	1 cut(s)	816
BspMI	ACCTGC	2 cut(s)	281, 1652
BspPI	GGATC	4 cut(s)	28, 456, 529, 2052
BspTNI	GGTCTC	1 cut(s)	196
BsrDI	GCAATG	2 cut(s)	1500, 2001
BsrFI	RCCGGY	1 cut(s)	1034
BsrGI	TGTACA	1 cut(s)	536
BsrI	ACTGG	4 cut(s)	1706, 1844, 1898, 1916
BssAI	RCCGGY	1 cut(s)	1034
BssECI	CCNNGG	2 cut(s)	186, 1828
BssNI	GRCGYC	1 cut(s)	183
BssT1I	CCWWGG	1 cut(s)	186
Bst2UI	CCWGG	1 cut(s)	1565
Bst4CI	ACNGT	9 cut(s)	171, 223, 861, 1226, 1710, 1981, 2038, 2188, 2199
BstACI	GRCGYC	1 cut(s)	183
BstAUI	TGTACA	1 cut(s)	536
BstBAI	YACGTR	1 cut(s)	155
BstC8I	GCNNGC	1 cut(s)	2095
BstEII	GGTNACC	1 cut(s)	307
BstF5I	GGATG	8 cut(s)	93, 421, 621, 984, 1086, 1363, 1722, 1937
BstFNI	CGCG	3 cut(s)	180, 1520, 2084
BstHHI	GCGC	2 cut(s)	609, 2086
BstMAI	GTCTC	6 cut(s)	30, 196, 242, 257, 368, 1947
BstMWI	GCNNNNNNNGC	5 cut(s)	287, 425, 685, 1767, 2090
BstNI	CCWGG	1 cut(s)	1565
BstNSI	RCATGY	1 cut(s)	1286
BstPI	GGTNACC	1 cut(s)	307
BstSCI	CCNGG	3 cut(s)	104, 1114, 1563
BstSFI	CTRYAG	1 cut(s)	781
BstUI	CGCG	3 cut(s)	180, 1520, 2084
BstV1I	GCAGC	6 cut(s)	838, 1183, 1484, 1492, 1783, 1978
BstV2I	GAAGAC	2 cut(s)	498, 1746
BstX2I	RGATCY	2 cut(s)	1747, 2044
BstYI	RGATCY	2 cut(s)	1747, 2044
BsuRI	GGCC	1 cut(s)	281
BtrI	CACGTC	1 cut(s)	1189
BtsCI	GGATG	8 cut(s)	93, 421, 621, 984, 1086, 1363, 1722, 1937
BtsIMutI	CAGTG	4 cut(s)	219, 1263, 1574, 1724
BveI	ACCTGC	2 cut(s)	281, 1652
Cac8I	GCNNGC	1 cut(s)	2095
CaiI	CAGNNNCTG	1 cut(s)	512
CciI	TCATGA	1 cut(s)	816
CfoI	GCGC	2 cut(s)	609, 2086
Cfr10I	RCCGGY	1 cut(s)	1034
Cfr13I	GGNCC	2 cut(s)	670, 1999
CseI	GACGC	1 cut(s)	191
Csp6I	GTAC	7 cut(s)	138, 172, 537, 1110, 1192, 1311, 1984
CviAII	CATG	8 cut(s)	445, 817, 1091, 1105, 1183, 1283, 1348, 1757
CviQI	GTAC	7 cut(s)	138, 172, 537, 1110, 1192, 1311, 1984
DraI	TTTAAA	1 cut(s)	276
DraIII	CACNNNGTG	1 cut(s)	550
DrdI	GACNNNNNNGTC	2 cut(s)	188, 212
DriI	GACNNNNNGTC	1 cut(s)	440
DseDI	GACNNNNNNGTC	2 cut(s)	188, 212
Eam1105I	GACNNNNNGTC	1 cut(s)	440
Ecl136II	GAGCTC	1 cut(s)	688
Eco130I	CCWWGG	1 cut(s)	186
Eco24I	GRGCYC	2 cut(s)	246, 690
Eco31I	GGTCTC	1 cut(s)	196
Eco47I	GGWCC	2 cut(s)	670, 1999
Eco53kI	GAGCTC	1 cut(s)	688
Eco57I	CTGAAG	2 cut(s)	716, 1700
Eco88I	CYCGRG	2 cut(s)	245, 627
Eco91I	GGTNACC	1 cut(s)	307
EcoICRI	GAGCTC	1 cut(s)	688
EcoO65I	GGTNACC	1 cut(s)	307
EcoRI	GAATTC	1 cut(s)	335
EcoRII	CCWGG	1 cut(s)	1563
EcoT14I	CCWWGG	1 cut(s)	186
EcoT38I	GRGCYC	2 cut(s)	246, 690
ErhI	CCWWGG	1 cut(s)	186
Esp3I	CGTCTC	1 cut(s)	242
FaeI	CATG	8 cut(s)	448, 820, 1094, 1108, 1186, 1286, 1351, 1760
FaqI	GGGAC	3 cut(s)	1131, 1910, 2174
FatI	CATG	8 cut(s)	444, 816, 1090, 1104, 1182, 1282, 1347, 1756
FauNDI	CATATG	1 cut(s)	1598
FbaI	TGATCA	1 cut(s)	1083
Fnu4HI	GCNGC	7 cut(s)	255, 852, 1197, 1498, 1506, 1797, 1992
FokI	GGATG	8 cut(s)	80, 428, 628, 991, 1073, 1370, 1709, 1944
FriOI	GRGCYC	2 cut(s)	246, 690
Fsp4HI	GCNGC	7 cut(s)	255, 852, 1197, 1498, 1506, 1797, 1992
FspBI	CTAG	3 cut(s)	326, 381, 1586
GlaI	GCGC	2 cut(s)	608, 2085
GluI	GCNGC	7 cut(s)	255, 852, 1197, 1498, 1506, 1797, 1992
GsuI	CTGGAG	2 cut(s)	1234, 2159
HaeIII	GGCC	1 cut(s)	281
HapII	CCGG	5 cut(s)	106, 282, 1035, 1115, 1532
HgaI	GACGC	1 cut(s)	191
HhaI	GCGC	2 cut(s)	609, 2086
Hin1I	GRCGYC	1 cut(s)	183
Hin1II	CATG	8 cut(s)	448, 820, 1094, 1108, 1186, 1286, 1351, 1760
Hin6I	GCGC	2 cut(s)	607, 2084
HinP1I	GCGC	2 cut(s)	607, 2084
HincII	GTYRAC	1 cut(s)	217
HindII	GTYRAC	1 cut(s)	217
HindIII	AAGCTT	4 cut(s)	51, 99, 761, 1958
HpaII	CCGG	5 cut(s)	106, 282, 1035, 1115, 1532
HphI	GGTGA	1 cut(s)	1378
Hpy166II	GTNNAC	6 cut(s)	217, 537, 1112, 1192, 1313, 1984
Hpy8I	GTNNAC	6 cut(s)	217, 537, 1112, 1192, 1313, 1984
Hpy99I	CGWCG	3 cut(s)	185, 1638, 1688
HpyAV	CCTTC	6 cut(s)	153, 157, 233, 477, 968, 1249
HpyCH4III	ACNGT	9 cut(s)	171, 223, 861, 1226, 1710, 1981, 2038, 2188, 2199
HpyCH4IV	ACGT	7 cut(s)	154, 269, 1012, 1025, 1188, 1877, 2160
HpyF10VI	GCNNNNNNNGC	5 cut(s)	287, 425, 685, 1767, 2090
HpySE526I	ACGT	7 cut(s)	154, 269, 1012, 1025, 1188, 1877, 2160
Hsp92I	GRCGYC	1 cut(s)	183
Hsp92II	CATG	8 cut(s)	448, 820, 1094, 1108, 1186, 1286, 1351, 1760
HspAI	GCGC	2 cut(s)	607, 2084
Kpn2I	TCCGGA	1 cut(s)	1531
Ksp22I	TGATCA	1 cut(s)	1083
LmnI	GCTCC	1 cut(s)	902
Lsp1109I	GCAGC	6 cut(s)	838, 1183, 1484, 1492, 1783, 1978
LweI	GCATC	6 cut(s)	406, 618, 1348, 1534, 1731, 1779
MaeI	CTAG	3 cut(s)	326, 381, 1586
MaeII	ACGT	7 cut(s)	154, 269, 1012, 1025, 1188, 1877, 2160
MaeIII	GTNAC	6 cut(s)	155, 223, 307, 1384, 1704, 2193
MflI	RGATCY	2 cut(s)	1747, 2044
MhlI	GDGCHC	2 cut(s)	246, 690
MluCI	AATT	7 cut(s)	335, 651, 692, 958, 1231, 2051, 2233
MlyI	GAGTC	2 cut(s)	1591, 1762
MmeI	TCCRAC	1 cut(s)	499
MroI	TCCGGA	1 cut(s)	1531
MroXI	GAANNNNTTC	2 cut(s)	906, 1943
MseI	TTAA	6 cut(s)	275, 654, 774, 989, 1337, 1446
MslI	CAYNNNNRTG	3 cut(s)	614, 1187, 1352
MspA1I	CMGCKG	2 cut(s)	803, 1497
MspI	CCGG	5 cut(s)	106, 282, 1035, 1115, 1532
MspR9I	CCNGG	3 cut(s)	106, 1116, 1565
MvaI	CCWGG	1 cut(s)	1565
MvnI	CGCG	3 cut(s)	180, 1520, 2084
MwoI	GCNNNNNNNGC	5 cut(s)	287, 425, 685, 1767, 2090
NciI	CCSGG	2 cut(s)	106, 1116
NdeI	CATATG	1 cut(s)	1598
NlaIII	CATG	8 cut(s)	448, 820, 1094, 1108, 1186, 1286, 1351, 1760
NmuCI	GTSAC	2 cut(s)	223, 1384
NspI	RCATGY	1 cut(s)	1286
PaeR7I	CTCGAG	2 cut(s)	245, 627
PagI	TCATGA	1 cut(s)	816
PaqCI	CACCTGC	2 cut(s)	281, 1652
PciI	ACATGT	1 cut(s)	1282
PcsI	WCGNNNNNNNCGW	1 cut(s)	1465
PdmI	GAANNNNTTC	2 cut(s)	906, 1943
PfeI	GAWTC	8 cut(s)	119, 199, 454, 479, 932, 1251, 1470, 1662
PfoI	TCCNGGA	1 cut(s)	104
PkrI	GCNGC	7 cut(s)	256, 853, 1198, 1499, 1507, 1798, 1993
PleI	GAGTC	2 cut(s)	1590, 1761
PpsI	GAGTC	2 cut(s)	1590, 1761
Ppu21I	YACGTR	1 cut(s)	155
PscI	ACATGT	1 cut(s)	1282
PsiI	TTATAA	1 cut(s)	1022
Psp124BI	GAGCTC	1 cut(s)	690
Psp1406I	AACGTT	3 cut(s)	1025, 1877, 2160
Psp6I	CCWGG	1 cut(s)	1563
PspEI	GGTNACC	1 cut(s)	307
PspGI	CCWGG	1 cut(s)	1563
PspPI	GGNCC	2 cut(s)	670, 1999
PsrI	GAACNNNNNNTAC	2 cut(s)	1801, 1833
PstNI	CAGNNNCTG	1 cut(s)	512
PsuI	RGATCY	2 cut(s)	1747, 2044
RsaI	GTAC	7 cut(s)	139, 173, 538, 1111, 1193, 1312, 1985
RsaNI	GTAC	7 cut(s)	138, 172, 537, 1110, 1192, 1311, 1984
RseI	CAYNNNNRTG	3 cut(s)	614, 1187, 1352
SacI	GAGCTC	1 cut(s)	690
SaqAI	TTAA	6 cut(s)	275, 654, 774, 989, 1337, 1446
SatI	GCNGC	7 cut(s)	255, 852, 1197, 1498, 1506, 1797, 1992
Sau96I	GGNCC	2 cut(s)	670, 1999
ScaI	AGTACT	1 cut(s)	139
SchI	GAGTC	2 cut(s)	1591, 1762
ScrFI	CCNGG	3 cut(s)	106, 1116, 1565
SduI	GDGCHC	2 cut(s)	246, 690
SfaNI	GCATC	6 cut(s)	406, 618, 1348, 1534, 1731, 1779
SfcI	CTRYAG	1 cut(s)	781
Sfr274I	CTCGAG	2 cut(s)	245, 627
SinI	GGWCC	2 cut(s)	670, 1999
SlaI	CTCGAG	2 cut(s)	245, 627
SmiMI	CAYNNNNRTG	3 cut(s)	614, 1187, 1352
SmlI	CTYRAG	7 cut(s)	245, 602, 627, 665, 710, 1948, 2099
SmoI	CTYRAG	7 cut(s)	245, 602, 627, 665, 710, 1948, 2099
Sse9I	AATT	7 cut(s)	335, 651, 692, 958, 1231, 2051, 2233
SsiI	CCGC	6 cut(s)	178, 254, 648, 801, 1495, 1520
SspI	AATATT	1 cut(s)	1657
SspMI	CTAG	3 cut(s)	326, 381, 1586
SstI	GAGCTC	1 cut(s)	690
StyD4I	CCNGG	3 cut(s)	104, 1114, 1563
StyI	CCWWGG	1 cut(s)	186
TaaI	ACNGT	9 cut(s)	171, 223, 861, 1226, 1710, 1981, 2038, 2188, 2199
TaiI	ACGT	7 cut(s)	157, 272, 1015, 1028, 1191, 1880, 2163
TaqI	TCGA	7 cut(s)	234, 246, 331, 587, 628, 766, 876
TaqII	GACCGA	1 cut(s)	1449
TasI	AATT	7 cut(s)	335, 651, 692, 958, 1231, 2051, 2233
TatI	WGTACW	3 cut(s)	137, 536, 1983
TauI	GCSGC	1 cut(s)	257
TfiI	GAWTC	8 cut(s)	119, 199, 454, 479, 932, 1251, 1470, 1662
Tru1I	TTAA	6 cut(s)	275, 654, 774, 989, 1337, 1446
Tru9I	TTAA	6 cut(s)	275, 654, 774, 989, 1337, 1446
TscAI	CASTG	4 cut(s)	226, 1270, 1581, 1724
TseFI	GTSAC	2 cut(s)	223, 1384
TseI	GCWGC	6 cut(s)	851, 1196, 1497, 1505, 1796, 1991
Tsp45I	GTSAC	2 cut(s)	223, 1384
TspDTI	ATGAA	7 cut(s)	632, 733, 833, 899, 931, 1231, 1669
TspGWI	ACGGA	1 cut(s)	1899
TspRI	CASTG	4 cut(s)	226, 1270, 1581, 1724
VpaK11BI	GGWCC	2 cut(s)	670, 1999
XapI	RAATTY	3 cut(s)	335, 692, 2051
XbaI	TCTAGA	1 cut(s)	1585
XceI	RCATGY	1 cut(s)	1286
XhoI	CTCGAG	2 cut(s)	245, 627
XmnI	GAANNNNTTC	2 cut(s)	906, 1943
XspI	CTAG	3 cut(s)	326, 381, 1586
ZrmI	AGTACT	1 cut(s)	139

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.