Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0010732

Physical Location & Seq

Genomic Coordinates Reverse (-)

4116056 .. 4120188

Gene Structure 4133 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1052300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2343 bp

ATGAAGTTCGGAAAAGAGTTTGTTTCGCAAATGGTGCCGGAATGGCAAGATGCATACATGGACTACAACTCTCTCAAGCTTATCTTAAAAGACATCCTGCGTTTCCGGAAGAAAAATGCATCATCGACGCCAATGGCGGCGACTTCGGCAGGGTCGACACTAAAGAGGAGGGTGTCACTCTATAGAGCATTCAGCGGGCTGACAAGCCGGCAACGGGGCTCGCTGAGGAAGAAGGAGGACGAGGAGATACTTGTGGGTGAAGAAGGTGAGGAAGGGCAGTGGCAGACAAGGTTTTTGATGCCGGCCGACGAGGGAGGAGAGTTTGAGGTTGTGTTTTTTAGGAGCCTGGACGATGAGTTTAACAAGGTGAATTGCTTTTACAAGAAGAAAGTTGGGGAAGTCGTGGAGGAGGCTGAGGAGTTGAATAGGCAGATGGATGCTTTGATTGCTCTTAGGTTAAAGGTTGATAATCCAGAGGTGGATATTGGAGGGAGTGATTTGGCAAGCAATGGGATTTCTTCAGTCTCAGCAGTTCATCCCACAAGTGGTAGAAAAACAGGGGGAGAAGATATGGATGTGATTAACGAAGTTGAGATGAGCGATGAAGAAGAAATGGAAGATGATGAAGAGAGGGGAGGCAAAGAATCGGAAACCGATGACTGGAAAGCCAACAGAGGGACGGTGGATATTCAGGGGCTTAAACCACCACAACTAGAGGTTCTAGATCATATAAAGATCAACATGATACCTGAAACTCCAGTGTCAACAATTAAAAGCATATTCCAGCAGAATTACTTATCATTCAGCAAGAAACAGCTGAGGAAAGTAGAAGAGCAAATGAGACAGGCTTTTAGTGAGTTCTATCAAAAGCTCCGGCTTTTAAAAAGCTACTGTTTCCTTAATCAATTGGCTTTCTCAAAGATCGTGAAGAAATATGACAAGTTCTCTTCAAGGAATGCATCGAAGGTTTACTTGAATATGGTGGATAATTCTTATCTCAGTAGCAGCGATGAAGTTACTAGGCTTATGGAAAGGGTGGAGACTACCTTCATAAAGCACTTCGCAAATGGAAATCGAAGAAAAGGAATGAAGACATTAAGGCCGAAAGCTAGAAGGGAAAAGCATAGAAGTACATTTTTCTTGGGGTTGCTCTCCGGGGGCTCCATTGCACTTGTAGTAGCCATCATTGTGCTTATGCATGCAAGAAATATCCTTGAGAGTGACGGGCGTGTTCTGTACATGGAAAACATATTTCCCCTGTACAGCCTGTTTGGATTCATTGTCCTACATCTGATCATGTTCTCAGCGAACATATTCTTTTGGAGGCGCTATCGTGTAAATTATCCATTTATCTTTGGCTTCAAGCAAGGGTCAGAGATTGGTTACAGACAGCTTTGCCTTCTCAGTTCAGGTATTGCAATTCTCGCATTGGCTGGTGTCATCTCAACTTTGGATATGGAGTTGGATCCAAGAACAAAAAGCTTCGTAGCCTTGAAAGAATTAGTCCCCCTGGGCCTAGTCATTGTTGTGCTTCTTATAATGTTTTGTCCTTTCAACGTTATATATCGTTCCAGTCGATATTTCCTCCTTCTATGCATATCCCATTGTCTTCTTGCTCCTCTTTATAAGGTCACCCTGCCGGATTTTTTCTTGGCAGATCAGCTTACTAGCCAGGTTCAAGCTTTCAGGAGTTTGGAATTCTATGTTTGCTATTACGGTTGGGGGGACTTCAAAAAGAGATCACACAACTGCCTCGACAGCCACGTTTTTAAATCCTTCTACTTTGTTGTAGCAATTATTCCTTATTGGATCCGTTCGCTTCAGTGTGTGCGACGCTTGATAGAGGAGAGAGATGGAATGCAAGGGTTAAATGCGCTGAAATACTTCTCAACAATCGTTGCAGTAGCCATGAGAACAAGTTATGGTCTGAAAGAAGGGATGACATCGAAAATCCTTGCTATAGTTAGTTCAGTTGTGGCAACTGTTGTCGGTACATATTGGGACATTGTCATCGATTGGGGTCTTCTGCAACGAAATTCTAAAAATCCTTGGCTGAGAGACAAACTCCTCGTATCAAACCACAGTGTTTATTTCATAGCCATGGTGTTGAATGTTGTACTGAGACTCGCTTGGATGCAGTCAGTACTGGGAATTAGAGAAGCGCCTTTTGTCCATAGAACAGCCTTGACTGCGATGGTTGCCTGCTTAGAGATCATTCGTCGTGGCATATGGAACTTCTTTCGGTTGGAGAATGAGCACTTGAACAATGTCGGAAAGTACCGTGCGTTCAAGTCTGTACCCCTACCCTTCAACTATGAACGTGATTACACCAGGAGCAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005802 GO:0005886 GO:0008150 GO:0012505 GO:0016020 GO:0031984 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050801 GO:0055062 GO:0055065 GO:0055069 GO:0055076 GO:0055080 GO:0055081 GO:0055083 GO:0065007 GO:0065008 GO:0071944 GO:0072505 GO:0072506 GO:0072507 GO:0098771 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EXS SPX

Protein Analysis

781

Amino Acids

89.75

Weight (kDa)

9.08

Isoelectric Point (pI)

41.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	1 - 328	3.9e-70	SPX domain
EXS	PF03124	417 - 754	1.2e-97	EXS family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010732)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G29060 AT4G25350
fragaria_vesca	FvH4_6g48060 FvH4_6g48060
malus_domestica	MD09G1054400.v1.1 MD17G1052300.v1.1
prunus_persica	Prupe.3G264100_v2.0.a1
pyrus_communis	pycom111g04570
rosa_chinensis	RchiOBHm_Chr2g0167381
rosa_laevigata	RLG00000021712 RLG00000021728
rosa_multiflora	Rmu_sc0001840.1_g000006
rosa_roxburghii	Rroxscaffold_2G00083560
rosa_rugosa	Rorug02G0529900.1 Rorug02G0530000
rosa_samantha	Rh2AG596800 Rh2BG608300 Rh2CG579100 Rh2DG619600
rosa_wichuraiana	Rw2G049680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1538, 1626
AccB1I	GGYRCC	1 cut(s)	34
AccI	GTMKAC	1 cut(s)	155
AccIII	TCCGGA	1 cut(s)	105
AciI	CCGC	2 cut(s)	137, 195
AclI	AACGTT	1 cut(s)	1557
AclWI	GGATC	4 cut(s)	1460, 1473, 1804, 1817
AcoI	YGGCCR	1 cut(s)	303
AcsI	RAATTY	2 cut(s)	1697, 2035
AcuI	CTGAAG	2 cut(s)	504, 1805
AcyI	GRCGYC	1 cut(s)	128
AfaI	GTAC	8 cut(s)	1132, 1238, 1262, 1993, 2118, 2145, 2279, 2298
AfiI	CCNNNNNNNGG	4 cut(s)	214, 545, 660, 675
AjnI	CCWGG	4 cut(s)	345, 1509, 1671, 2330
Alw21I	GWGCWC	1 cut(s)	2259
Alw26I	GTCTC	5 cut(s)	529, 835, 1034, 2052, 2116
AlwI	GGATC	4 cut(s)	1460, 1473, 1804, 1817
Aor13HI	TCCGGA	1 cut(s)	105
AoxI	GGCC	3 cut(s)	303, 1100, 1513
ApeKI	GCWGC	2 cut(s)	1005, 2337
ApoI	RAATTY	2 cut(s)	1697, 2035
ArsI	GACNNNNNNTTYG	2 cut(s)	277, 309
Asp700I	GAANNNNTTC	2 cut(s)	1313, 1883
AspLEI	GCGC	3 cut(s)	1329, 1876, 2164
AspS9I	GGNCC	1 cut(s)	1513
AsuC2I	CCSGG	1 cut(s)	1156
AsuHPI	GGTGA	4 cut(s)	269, 278, 379, 1624
BamHI	GGATCC	2 cut(s)	1465, 1809
BanI	GGYRCC	1 cut(s)	34
BanII	GRGCYC	2 cut(s)	221, 1163
BbsI	GAAGAC	3 cut(s)	1097, 1601, 2015
Bbv12I	GWGCWC	1 cut(s)	2259
BbvCI	CCTCAGC	3 cut(s)	224, 414, 818
BbvI	GCAGC	1 cut(s)	1017
BccI	CCATC	4 cut(s)	427, 1190, 1847, 2188
BciT130I	CCWGG	4 cut(s)	347, 1511, 1673, 2332
BclI	TGATCA	1 cut(s)	1293
BcnI	CCSGG	1 cut(s)	1156
BcoDI	GTCTC	5 cut(s)	529, 835, 1034, 2052, 2116
BfaI	CTAG	6 cut(s)	713, 722, 1020, 1110, 1517, 1668
BfmI	CTRYAG	2 cut(s)	181, 1959
BfoI	RGCGCY	2 cut(s)	1330, 2165
BglI	GCCNNNNNGGC	1 cut(s)	43
BisI	GCNGC	3 cut(s)	138, 1006, 2338
BlsI	GCNGC	3 cut(s)	139, 1007, 2339
BmcAI	AGTACT	1 cut(s)	2145
Bme1390I	CCNGG	5 cut(s)	347, 1156, 1511, 1673, 2332
BmgT120I	GGNCC	1 cut(s)	1513
BmiI	GGNNCC	5 cut(s)	36, 344, 1162, 1467, 1811
BmrFI	CCNGG	5 cut(s)	347, 1156, 1511, 1673, 2332
BmrI	ACTGGG	1 cut(s)	2156
BmsI	GCATC	6 cut(s)	40, 128, 288, 427, 968, 2124
BmuI	ACTGGG	1 cut(s)	2156
BpiI	GAAGAC	3 cut(s)	1097, 1601, 2015
BplI	GAGNNNNNCTC	2 cut(s)	2049, 2081
BpmI	CTGGAG	1 cut(s)	741
Bpu10I	CCTNAGC	3 cut(s)	224, 414, 818
BpuEI	CTTGAG	2 cut(s)	59, 1235
BpuMI	CCSGG	1 cut(s)	1156
Bsa29I	ATCGAT	1 cut(s)	2013
BsaHI	GRCGYC	1 cut(s)	128
BsaJI	CCNNGG	5 cut(s)	1155, 1509, 1510, 2048, 2100
BsaWI	WCCGGW	1 cut(s)	105
Bsc4I	CCNNNNNNNGG	4 cut(s)	214, 545, 660, 675
Bse118I	RCCGGY	2 cut(s)	207, 301
Bse1I	ACTGG	4 cut(s)	665, 758, 1572, 2151
Bse3DI	GCAATG	2 cut(s)	514, 1164
BseAI	TCCGGA	1 cut(s)	105
BseBI	CCWGG	4 cut(s)	347, 1511, 1673, 2332
BseCI	ATCGAT	1 cut(s)	2013
BseDI	CCNNGG	5 cut(s)	1155, 1509, 1510, 2048, 2100
BseGI	GGATG	6 cut(s)	93, 442, 535, 580, 1944, 2139
BseLI	CCNNNNNNNGG	4 cut(s)	214, 545, 660, 675
BseMI	GCAATG	2 cut(s)	514, 1164
BseMII	CTCAG	9 cut(s)	215, 405, 540, 809, 1012, 1317, 1417, 2045, 2111
BseNI	ACTGG	4 cut(s)	665, 758, 1572, 2151
BseRI	GAGGAG	8 cut(s)	181, 257, 330, 422, 431, 1608, 1859, 2057
BseX3I	CGGCCG	1 cut(s)	303
BseXI	GCAGC	1 cut(s)	1017
Bsh1285I	CGRYCG	1 cut(s)	306
BshFI	GGCC	3 cut(s)	305, 1102, 1515
BshNI	GGYRCC	1 cut(s)	34
BshVI	ATCGAT	1 cut(s)	2013
BsiEI	CGRYCG	1 cut(s)	306
BsiHKAI	GWGCWC	1 cut(s)	2259
BsiSI	CCGG	7 cut(s)	38, 106, 208, 302, 874, 1155, 1640
BslFI	GGGAC	4 cut(s)	691, 1490, 1739, 2015
BslI	CCNNNNNNNGG	4 cut(s)	214, 545, 660, 675
BsmAI	GTCTC	5 cut(s)	529, 835, 1034, 2052, 2116
BsmFI	GGGAC	4 cut(s)	691, 1490, 1739, 2015
BsmI	GAATGC	3 cut(s)	188, 961, 1863
BsnI	GGCC	3 cut(s)	305, 1102, 1515
Bsp1286I	GDGCHC	3 cut(s)	221, 1163, 2259
Bsp13I	TCCGGA	1 cut(s)	105
Bsp1407I	TGTACA	2 cut(s)	1236, 1260
Bsp143I	GATC	9 cut(s)	724, 735, 921, 1293, 1465, 1657, 1739, 1809, 2211
Bsp19I	CCATGG	1 cut(s)	2100
BspACI	CCGC	2 cut(s)	137, 195
BspANI	GGCC	3 cut(s)	305, 1102, 1515
BspCNI	CTCAG	9 cut(s)	216, 406, 539, 810, 1011, 1316, 1416, 2046, 2112
BspDI	ATCGAT	1 cut(s)	2013
BspEI	TCCGGA	1 cut(s)	105
BspLI	GGNNCC	5 cut(s)	36, 344, 1162, 1467, 1811
BspPI	GGATC	4 cut(s)	1460, 1473, 1804, 1817
BspQI	GCTCTTC	1 cut(s)	825
BspT107I	GGYRCC	1 cut(s)	34
BsrDI	GCAATG	2 cut(s)	514, 1164
BsrFI	RCCGGY	2 cut(s)	207, 301
BsrGI	TGTACA	2 cut(s)	1236, 1260
BsrI	ACTGG	4 cut(s)	665, 758, 1572, 2151
BssAI	RCCGGY	2 cut(s)	207, 301
BssECI	CCNNGG	5 cut(s)	1155, 1509, 1510, 2048, 2100
BssMI	GATC	9 cut(s)	724, 735, 921, 1293, 1465, 1657, 1739, 1809, 2211
BssNI	GRCGYC	1 cut(s)	128
BssT1I	CCWWGG	2 cut(s)	2048, 2100
Bst2UI	CCWGG	4 cut(s)	347, 1511, 1673, 2332
Bst4CI	ACNGT	6 cut(s)	682, 893, 1718, 1984, 2084, 2282
Bst6I	CTCTTC	3 cut(s)	621, 825, 952
BstACI	GRCGYC	1 cut(s)	128
BstAPI	GCANNNNNTGC	1 cut(s)	34
BstAUI	TGTACA	2 cut(s)	1236, 1260
BstC8I	GCNNGC	7 cut(s)	197, 209, 221, 303, 505, 1200, 2203
BstDSI	CCRYGG	1 cut(s)	2100
BstEII	GGTNACC	1 cut(s)	1630
BstF5I	GGATG	6 cut(s)	93, 442, 535, 580, 1944, 2139
BstH2I	RGCGCY	2 cut(s)	1330, 2165
BstHHI	GCGC	3 cut(s)	1329, 1876, 2164
BstKTI	GATC	9 cut(s)	727, 738, 924, 1296, 1468, 1660, 1742, 1812, 2214
BstMAI	GTCTC	5 cut(s)	529, 835, 1034, 2052, 2116
BstMBI	GATC	9 cut(s)	724, 735, 921, 1293, 1465, 1657, 1739, 1809, 2211
BstMCI	CGRYCG	1 cut(s)	306
BstMWI	GCNNNNNNNGC	8 cut(s)	34, 43, 146, 446, 1424, 1758, 2189, 2198
BstNI	CCWGG	4 cut(s)	347, 1511, 1673, 2332
BstNSI	RCATGY	1 cut(s)	1202
BstPI	GGTNACC	1 cut(s)	1630
BstSCI	CCNGG	5 cut(s)	345, 1154, 1509, 1671, 2330
BstSFI	CTRYAG	2 cut(s)	181, 1959
BstV1I	GCAGC	1 cut(s)	1017
BstV2I	GAAGAC	3 cut(s)	1097, 1601, 2015
BstX2I	RGATCY	2 cut(s)	1465, 1809
BstYI	RGATCY	2 cut(s)	1465, 1809
BstZI	CGGCCG	1 cut(s)	303
Bsu15I	ATCGAT	1 cut(s)	2013
BsuRI	GGCC	3 cut(s)	305, 1102, 1515
BsuTUI	ATCGAT	1 cut(s)	2013
BtgI	CCRYGG	1 cut(s)	2100
BtgZI	GCGATG	3 cut(s)	615, 1023, 2207
BtsCI	GGATG	6 cut(s)	93, 442, 535, 580, 1944, 2139
BtsI	GCAGTG	1 cut(s)	284
BtsIMutI	CAGTG	4 cut(s)	284, 765, 1829, 2089
Cac8I	GCNNGC	7 cut(s)	197, 209, 221, 303, 505, 1200, 2203
CfoI	GCGC	3 cut(s)	1329, 1876, 2164
Cfr10I	RCCGGY	2 cut(s)	207, 301
Cfr13I	GGNCC	1 cut(s)	1513
ClaI	ATCGAT	1 cut(s)	2013
CseI	GACGC	2 cut(s)	136, 1842
Csp6I	GTAC	8 cut(s)	1131, 1237, 1261, 1992, 2117, 2144, 2278, 2297
CviAII	CATG	7 cut(s)	58, 742, 1199, 1240, 1297, 1909, 2101
CviQI	GTAC	8 cut(s)	1131, 1237, 1261, 1992, 2117, 2144, 2278, 2297
DpnI	GATC	9 cut(s)	726, 737, 923, 1295, 1467, 1659, 1741, 1811, 2213
DpnII	GATC	9 cut(s)	724, 735, 921, 1293, 1465, 1657, 1739, 1809, 2211
DraI	TTTAAA	2 cut(s)	882, 1771
EaeI	YGGCCR	1 cut(s)	303
EagI	CGGCCG	1 cut(s)	303
Eam1104I	CTCTTC	3 cut(s)	621, 825, 952
EarI	CTCTTC	3 cut(s)	621, 825, 952
EclXI	CGGCCG	1 cut(s)	303
Eco130I	CCWWGG	2 cut(s)	2048, 2100
Eco24I	GRGCYC	2 cut(s)	221, 1163
Eco52I	CGGCCG	1 cut(s)	303
Eco57I	CTGAAG	2 cut(s)	504, 1805
Eco91I	GGTNACC	1 cut(s)	1630
EcoO65I	GGTNACC	1 cut(s)	1630
EcoRI	GAATTC	1 cut(s)	1697
EcoRII	CCWGG	4 cut(s)	345, 1509, 1671, 2330
EcoT14I	CCWWGG	2 cut(s)	2048, 2100
EcoT22I	ATGCAT	5 cut(s)	55, 121, 961, 1200, 1598
EcoT38I	GRGCYC	2 cut(s)	221, 1163
ErhI	CCWWGG	2 cut(s)	2048, 2100
FaeI	CATG	7 cut(s)	61, 745, 1202, 1243, 1300, 1912, 2104
FalI	AAGNNNNNCTT	4 cut(s)	68, 100, 956, 988
FaqI	GGGAC	4 cut(s)	691, 1490, 1739, 2015
FatI	CATG	7 cut(s)	57, 741, 1198, 1239, 1296, 1908, 2100
FauI	CCCGC	1 cut(s)	188
FauNDI	CATATG	1 cut(s)	2228
FbaI	TGATCA	1 cut(s)	1293
FblI	GTMKAC	1 cut(s)	155
Fnu4HI	GCNGC	3 cut(s)	138, 1006, 2338
FokI	GGATG	6 cut(s)	80, 449, 522, 587, 1951, 2146
FriOI	GRGCYC	2 cut(s)	221, 1163
Fsp4HI	GCNGC	3 cut(s)	138, 1006, 2338
FspBI	CTAG	6 cut(s)	713, 722, 1020, 1110, 1517, 1668
GlaI	GCGC	3 cut(s)	1328, 1875, 2163
GluI	GCNGC	3 cut(s)	138, 1006, 2338
GsuI	CTGGAG	1 cut(s)	741
HaeII	RGCGCY	2 cut(s)	1330, 2165
HaeIII	GGCC	3 cut(s)	305, 1102, 1515
HapII	CCGG	7 cut(s)	38, 106, 208, 302, 874, 1155, 1640
HgaI	GACGC	2 cut(s)	136, 1842
HhaI	GCGC	3 cut(s)	1329, 1876, 2164
Hin1I	GRCGYC	1 cut(s)	128
Hin1II	CATG	7 cut(s)	61, 745, 1202, 1243, 1300, 1912, 2104
Hin6I	GCGC	3 cut(s)	1327, 1874, 2162
HinP1I	GCGC	3 cut(s)	1327, 1874, 2162
HincII	GTYRAC	2 cut(s)	156, 765
HindII	GTYRAC	2 cut(s)	156, 765
HindIII	AAGCTT	3 cut(s)	77, 1480, 1680
HinfI	GANTC	3 cut(s)	644, 1275, 2124
HpaII	CCGG	7 cut(s)	38, 106, 208, 302, 874, 1155, 1640
HphI	GGTGA	4 cut(s)	269, 278, 379, 1624
Hpy166II	GTNNAC	3 cut(s)	156, 765, 970
Hpy188I	TCNGA	6 cut(s)	11, 649, 1293, 1375, 1929, 2273
Hpy188III	TCNNGA	5 cut(s)	106, 473, 722, 925, 1687
Hpy8I	GTNNAC	3 cut(s)	156, 765, 970
Hpy99I	CGWCG	4 cut(s)	130, 311, 1836, 2223
HpyCH4III	ACNGT	6 cut(s)	682, 893, 1718, 1984, 2084, 2282
HpyCH4IV	ACGT	3 cut(s)	1557, 1764, 2320
HpyF10VI	GCNNNNNNNGC	8 cut(s)	34, 43, 146, 446, 1424, 1758, 2189, 2198
HpySE526I	ACGT	3 cut(s)	1557, 1764, 2320
Hsp92I	GRCGYC	1 cut(s)	128
Hsp92II	CATG	7 cut(s)	61, 745, 1202, 1243, 1300, 1912, 2104
HspAI	GCGC	3 cut(s)	1327, 1874, 2162
Kpn2I	TCCGGA	1 cut(s)	105
KroI	GCCGGC	2 cut(s)	207, 301
KroNI	GCCGGC	2 cut(s)	209, 303
Ksp22I	TGATCA	1 cut(s)	1293
Kzo9I	GATC	9 cut(s)	724, 735, 921, 1293, 1465, 1657, 1739, 1809, 2211
LguI	GCTCTTC	1 cut(s)	825
LmnI	GCTCC	5 cut(s)	342, 876, 1166, 1621, 2334
Lsp1109I	GCAGC	1 cut(s)	1017
LweI	GCATC	6 cut(s)	40, 128, 288, 427, 968, 2124
MaeI	CTAG	6 cut(s)	713, 722, 1020, 1110, 1517, 1668
MaeII	ACGT	3 cut(s)	1557, 1764, 2320
MaeIII	GTNAC	5 cut(s)	174, 1015, 1220, 1382, 1630
MalI	GATC	9 cut(s)	726, 737, 923, 1295, 1467, 1659, 1741, 1811, 2213
MboI	GATC	9 cut(s)	724, 735, 921, 1293, 1465, 1657, 1739, 1809, 2211
MfeI	CAATTG	1 cut(s)	905
MflI	RGATCY	2 cut(s)	1465, 1809
MhlI	GDGCHC	3 cut(s)	221, 1163, 2259
MlyI	GAGTC	1 cut(s)	2118
MmeI	TCCRAC	3 cut(s)	1443, 2226, 2251
Mph1103I	ATGCAT	5 cut(s)	55, 121, 961, 1200, 1598
MroI	TCCGGA	1 cut(s)	105
MroNI	GCCGGC	2 cut(s)	207, 301
MroXI	GAANNNNTTC	2 cut(s)	1313, 1883
MslI	CAYNNNNRTG	3 cut(s)	1187, 1526, 2099
MspA1I	CMGCKG	3 cut(s)	195, 817, 2340
MspI	CCGG	7 cut(s)	38, 106, 208, 302, 874, 1155, 1640
MspR9I	CCNGG	5 cut(s)	347, 1156, 1511, 1673, 2332
MunI	CAATTG	1 cut(s)	905
Mva1269I	GAATGC	3 cut(s)	188, 961, 1863
MvaI	CCWGG	4 cut(s)	347, 1511, 1673, 2332
MwoI	GCNNNNNNNGC	8 cut(s)	34, 43, 146, 446, 1424, 1758, 2189, 2198
NaeI	GCCGGC	2 cut(s)	209, 303
NciI	CCSGG	1 cut(s)	1156
NcoI	CCATGG	1 cut(s)	2100
NdeI	CATATG	1 cut(s)	2228
NdeII	GATC	9 cut(s)	724, 735, 921, 1293, 1465, 1657, 1739, 1809, 2211
NgoMIV	GCCGGC	2 cut(s)	207, 301
NlaIII	CATG	7 cut(s)	61, 745, 1202, 1243, 1300, 1912, 2104
NlaIV	GGNNCC	5 cut(s)	36, 344, 1162, 1467, 1811
NmuCI	GTSAC	3 cut(s)	174, 1220, 1630
NsiI	ATGCAT	5 cut(s)	55, 121, 961, 1200, 1598
NspI	RCATGY	1 cut(s)	1202
PaeI	GCATGC	1 cut(s)	1202
PasI	CCCWGGG	1 cut(s)	1510
PciSI	GCTCTTC	1 cut(s)	825
PcsI	WCGNNNNNNNCGW	3 cut(s)	152, 1573, 1761
PctI	GAATGC	3 cut(s)	188, 961, 1863
PdiI	GCCGGC	2 cut(s)	209, 303
PdmI	GAANNNNTTC	2 cut(s)	1313, 1883
PfeI	GAWTC	2 cut(s)	644, 1275
PflFI	GACNNNGTC	1 cut(s)	2006
PkrI	GCNGC	3 cut(s)	139, 1007, 2339
PleI	GAGTC	1 cut(s)	2118
PpsI	GAGTC	1 cut(s)	2118
PsiI	TTATAA	2 cut(s)	1538, 1626
Psp1406I	AACGTT	1 cut(s)	1557
Psp6I	CCWGG	4 cut(s)	345, 1509, 1671, 2330
PspEI	GGTNACC	1 cut(s)	1630
PspGI	CCWGG	4 cut(s)	345, 1509, 1671, 2330
PspN4I	GGNNCC	5 cut(s)	36, 344, 1162, 1467, 1811
PspPI	GGNCC	1 cut(s)	1513
PsrI	GAACNNNNNNTAC	2 cut(s)	2270, 2302
PsuI	RGATCY	2 cut(s)	1465, 1809
PsyI	GACNNNGTC	1 cut(s)	2006
PvuII	CAGCTG	2 cut(s)	817, 2340
RsaI	GTAC	8 cut(s)	1132, 1238, 1262, 1993, 2118, 2145, 2279, 2298
RsaNI	GTAC	8 cut(s)	1131, 1237, 1261, 1992, 2117, 2144, 2278, 2297
RseI	CAYNNNNRTG	3 cut(s)	1187, 1526, 2099
SalI	GTCGAC	1 cut(s)	154
SapI	GCTCTTC	1 cut(s)	825
SatI	GCNGC	3 cut(s)	138, 1006, 2338
Sau3AI	GATC	9 cut(s)	724, 735, 921, 1293, 1465, 1657, 1739, 1809, 2211
Sau96I	GGNCC	1 cut(s)	1513
ScaI	AGTACT	1 cut(s)	2145
SchI	GAGTC	1 cut(s)	2118
ScrFI	CCNGG	5 cut(s)	347, 1156, 1511, 1673, 2332
SduI	GDGCHC	3 cut(s)	221, 1163, 2259
SfaNI	GCATC	6 cut(s)	40, 128, 288, 427, 968, 2124
SfcI	CTRYAG	2 cut(s)	181, 1959
SmiMI	CAYNNNNRTG	3 cut(s)	1187, 1526, 2099
SmlI	CTYRAG	2 cut(s)	74, 1214
SmoI	CTYRAG	2 cut(s)	74, 1214
SphI	GCATGC	1 cut(s)	1202
SsiI	CCGC	2 cut(s)	137, 195
SspMI	CTAG	6 cut(s)	713, 722, 1020, 1110, 1517, 1668
StyD4I	CCNGG	5 cut(s)	345, 1154, 1509, 1671, 2330
StyI	CCWWGG	2 cut(s)	2048, 2100
TaaI	ACNGT	6 cut(s)	682, 893, 1718, 1984, 2084, 2282
TaiI	ACGT	3 cut(s)	1560, 1767, 2323
TaqI	TCGA	8 cut(s)	125, 155, 962, 1075, 1576, 1755, 1946, 2013
TatI	WGTACW	5 cut(s)	1130, 1236, 1260, 2116, 2143
TauI	GCSGC	1 cut(s)	140
TfiI	GAWTC	2 cut(s)	644, 1275
TscAI	CASTG	4 cut(s)	284, 765, 1829, 2089
TseFI	GTSAC	3 cut(s)	174, 1220, 1630
TseI	GCWGC	2 cut(s)	1005, 2337
Tsp45I	GTSAC	3 cut(s)	174, 1220, 1630
TspGWI	ACGGA	1 cut(s)	1802
TspRI	CASTG	4 cut(s)	284, 765, 1829, 2089
Tth111I	GACNNNGTC	1 cut(s)	2006
XapI	RAATTY	2 cut(s)	1697, 2035
XbaI	TCTAGA	1 cut(s)	721
XceI	RCATGY	1 cut(s)	1202
XmiI	GTMKAC	1 cut(s)	155
XmnI	GAANNNNTTC	2 cut(s)	1313, 1883
XspI	CTAG	6 cut(s)	713, 722, 1020, 1110, 1517, 1668
ZrmI	AGTACT	1 cut(s)	2145
Zsp2I	ATGCAT	5 cut(s)	55, 121, 961, 1200, 1598

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.