AT4G27435

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014323
Physical Location & Seq
Forward (+)
13723610 .. 13725359
1750 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27435.1

Sequence Viewer

Length: 522 bp
ATGGCGTCCAAGATTGTCTCAGCCATAGTATTTGTGTTTAACCTCATTGCCTTTGGTTTAGCCGTTGCTGCTGAACAAAGAAGAAGCACTGCAAGGGTTGTGCAGGACACTGAGGTGCAATATAACTACTGTGTGTATGACTCGGACAGAGCTACAGGGTATGGAGTTGGAGCCTTTTTGTTCTCAGTGGCTAGTCAAATCCTTATCATGCTCGTTAGCCGTTGCTTCTGTTGTGGAAAGCCTCTCAAGCCTGGCGGTTCACGCGCTCTTGCCCTCATTCTCTTCATTGTTAGCTGGATGTTCTTCTTGATAGCGGAGATATGTCTATTAGCTGGATCGGTAGAGAATGCATACCACACAAAGTACAGGACAATGTTCATGGACAATCCTCCTGATTGTCAAACTCTACGTAAAGGCGTCTTTGCAGCAGGCGCCTCATTCGTCTTCTTCAACGCCATTGTCTCTCAGTTCTACTATTTCTTCTATTTCTCTGCTGCTGAGGCCTCTCTTTCACCTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

173

Amino Acids

19.18

Weight (kDa)

8.54

Isoelectric Point (pI)

30.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 152 7.5e-31 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014323)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27435
fragaria_vesca FvH4_3g20620
malus_domestica MD03G1223600.v1.1 MD11G1241500.v1.1
prunus_persica Prupe.4G185900_v2.0.a1
pyrus_communis pycom03g17190 pycom11g21310
rosa_chinensis RchiOBHm_Chr5g0034641
rosa_laevigata RLG00000033562
rosa_multiflora Rmu_sc0002460.1_g000087
rosa_roxburghii Rroxscaffold_1G00045680
rosa_rugosa Rorug05G0147400 Rorug05G0147400
rosa_samantha Rh5AG239400 Rh5BG240100 Rh5DG248300
rosa_wichuraiana Rw5G021940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 431
AccII CGCG 1 cut(s) 264
AciI CCGC 2 cut(s) 255, 314
AclWI GGATC 1 cut(s) 343
AcyI GRCGYC 3 cut(s) 5, 417, 432
AfaI GTAC 1 cut(s) 365
AgsI TTSAA 1 cut(s) 451
AjnI CCWGG 1 cut(s) 250
AleI CACNNNNGTG 1 cut(s) 113
AluBI AGCT 3 cut(s) 152, 294, 332
AluI AGCT 3 cut(s) 152, 294, 332
Alw26I GTCTC 2 cut(s) 22, 466
AlwI GGATC 1 cut(s) 343
AoxI GGCC 1 cut(s) 501
ApeKI GCWGC 3 cut(s) 68, 425, 494
AspLEI GCGC 2 cut(s) 266, 434
AsuHPI GGTGA 1 cut(s) 504
BanI GGYRCC 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 436
BbvCI CCTCAGC 1 cut(s) 498
BbvI GCAGC 3 cut(s) 55, 437, 481
BceAI ACGGC 2 cut(s) 47, 204
BciT130I CCWGG 1 cut(s) 252
BcoDI GTCTC 2 cut(s) 22, 466
BfaI CTAG 2 cut(s) 192, 520
BfmI CTRYAG 1 cut(s) 153
BfoI RGCGCY 1 cut(s) 435
BisI GCNGC 3 cut(s) 69, 426, 495
BlsI GCNGC 3 cut(s) 70, 427, 496
Bme1390I CCNGG 1 cut(s) 252
BmiI GGNNCC 2 cut(s) 172, 433
BmrFI CCNGG 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 436
Bpu10I CCTNAGC 1 cut(s) 498
BpuEI CTTGAG 1 cut(s) 230
BsaAI YACGTR 1 cut(s) 410
BsaHI GRCGYC 3 cut(s) 5, 417, 432
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 1 cut(s) 252
BseGI GGATG 1 cut(s) 303
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 5 cut(s) 33, 102, 198, 479, 489
BseXI GCAGC 3 cut(s) 55, 437, 481
BsgI GTGCAG 1 cut(s) 122
Bsh1236I CGCG 1 cut(s) 264
BshFI GGCC 1 cut(s) 503
BshNI GGYRCC 1 cut(s) 431
BsmAI GTCTC 2 cut(s) 22, 466
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 1 cut(s) 503
Bsp143I GATC 1 cut(s) 335
BspACI CCGC 2 cut(s) 255, 314
BspANI GGCC 1 cut(s) 503
BspCNI CTCAG 5 cut(s) 32, 103, 197, 478, 490
BspFNI CGCG 1 cut(s) 264
BspLI GGNNCC 2 cut(s) 172, 433
BspPI GGATC 1 cut(s) 343
BspT107I GGYRCC 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 45
BssMI GATC 1 cut(s) 335
BssNI GRCGYC 3 cut(s) 5, 417, 432
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 1 cut(s) 131
Bst6I CTCTTC 1 cut(s) 287
BstACI GRCGYC 3 cut(s) 5, 417, 432
BstBAI YACGTR 1 cut(s) 410
BstC8I GCNNGC 1 cut(s) 430
BstDEI CTNAG 5 cut(s) 19, 111, 184, 465, 498
BstF5I GGATG 1 cut(s) 303
BstFNI CGCG 1 cut(s) 264
BstH2I RGCGCY 1 cut(s) 435
BstHHI GCGC 2 cut(s) 266, 434
BstKTI GATC 1 cut(s) 338
BstMAI GTCTC 2 cut(s) 22, 466
BstMBI GATC 1 cut(s) 335
BstMWI GCNNNNNNNGC 5 cut(s) 68, 247, 261, 431, 500
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 250
BstSFI CTRYAG 1 cut(s) 153
BstSNI TACGTA 1 cut(s) 410
BstUI CGCG 1 cut(s) 264
BstV1I GCAGC 3 cut(s) 55, 437, 481
BstV2I GAAGAC 1 cut(s) 436
BsuRI GGCC 1 cut(s) 503
BtsCI GGATG 1 cut(s) 303
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 3 cut(s) 87, 108, 192
Cac8I GCNNGC 1 cut(s) 430
CfoI GCGC 2 cut(s) 266, 434
CseI GACGC 1 cut(s) 406
Csp6I GTAC 1 cut(s) 364
CviAII CATG 2 cut(s) 208, 379
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 5 cut(s) 19, 111, 184, 465, 498
DinI GGCGCC 1 cut(s) 433
DpnI GATC 1 cut(s) 337
DpnII GATC 1 cut(s) 335
Eam1104I CTCTTC 1 cut(s) 287
EarI CTCTTC 1 cut(s) 287
Eco105I TACGTA 1 cut(s) 410
Eco147I AGGCCT 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 250
EcoT22I ATGCAT 1 cut(s) 352
EgeI GGCGCC 1 cut(s) 433
EheI GGCGCC 1 cut(s) 433
FaeI CATG 2 cut(s) 211, 382
FaiI YATR 8 cut(s) 26, 123, 138, 162, 209, 322, 352, 380
FatI CATG 2 cut(s) 207, 378
Fnu4HI GCNGC 3 cut(s) 69, 426, 495
FokI GGATG 1 cut(s) 310
Fsp4HI GCNGC 3 cut(s) 69, 426, 495
FspBI CTAG 2 cut(s) 192, 520
GlaI GCGC 2 cut(s) 265, 433
GluI GCNGC 3 cut(s) 69, 426, 495
HaeII RGCGCY 1 cut(s) 435
HaeIII GGCC 1 cut(s) 503
HgaI GACGC 1 cut(s) 406
HhaI GCGC 2 cut(s) 266, 434
Hin1I GRCGYC 3 cut(s) 5, 417, 432
Hin1II CATG 2 cut(s) 211, 382
Hin6I GCGC 2 cut(s) 264, 432
HinP1I GCGC 2 cut(s) 264, 432
HinfI GANTC 1 cut(s) 140
HphI GGTGA 1 cut(s) 504
Hpy166II GTNNAC 1 cut(s) 260
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 2 cut(s) 307, 392
Hpy8I GTNNAC 1 cut(s) 260
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4IV ACGT 1 cut(s) 409
HpyCH4V TGCA 5 cut(s) 92, 103, 118, 350, 425
HpyF10VI GCNNNNNNNGC 5 cut(s) 68, 247, 261, 431, 500
HpyF3I CTNAG 5 cut(s) 19, 111, 184, 465, 498
HpySE526I ACGT 1 cut(s) 409
Hsp92I GRCGYC 3 cut(s) 5, 417, 432
Hsp92II CATG 2 cut(s) 211, 382
HspAI GCGC 2 cut(s) 264, 432
KasI GGCGCC 1 cut(s) 431
Kzo9I GATC 1 cut(s) 335
LmnI GCTCC 1 cut(s) 170
LpnPI CCDG 9 cut(s) 89, 141, 237, 264, 280, 318, 352, 405, 414
Lsp1109I GCAGC 3 cut(s) 55, 437, 481
MaeI CTAG 2 cut(s) 192, 520
MaeII ACGT 1 cut(s) 409
MalI GATC 1 cut(s) 337
MboI GATC 1 cut(s) 335
MboII GAAGA 6 cut(s) 93, 274, 295, 436, 439, 472
Mly113I GGCGCC 1 cut(s) 432
MlyI GAGTC 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 148
MnlI CCTC 8 cut(s) 53, 106, 252, 284, 399, 445, 493, 514
Mph1103I ATGCAT 1 cut(s) 352
MseI TTAA 1 cut(s) 39
MslI CAYNNNNRTG 1 cut(s) 113
MspR9I CCNGG 1 cut(s) 252
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 1 cut(s) 252
MvnI CGCG 1 cut(s) 264
MwoI GCNNNNNNNGC 5 cut(s) 68, 247, 261, 431, 500
NarI GGCGCC 1 cut(s) 432
NdeII GATC 1 cut(s) 335
NlaIII CATG 2 cut(s) 211, 382
NlaIV GGNNCC 2 cut(s) 172, 433
NsiI ATGCAT 1 cut(s) 352
OliI CACNNNNGTG 1 cut(s) 113
PceI AGGCCT 1 cut(s) 503
PctI GAATGC 1 cut(s) 352
PkrI GCNGC 3 cut(s) 70, 427, 496
PleI GAGTC 1 cut(s) 134
PluTI GGCGCC 1 cut(s) 435
PpsI GAGTC 1 cut(s) 134
Ppu21I YACGTR 1 cut(s) 410
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 2 cut(s) 172, 433
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 1 cut(s) 113
SaqAI TTAA 1 cut(s) 39
SatI GCNGC 3 cut(s) 69, 426, 495
Sau3AI GATC 1 cut(s) 335
SchI GAGTC 1 cut(s) 134
ScrFI CCNGG 1 cut(s) 252
SetI ASST 7 cut(s) 45, 117, 154, 296, 334, 412, 517
SfcI CTRYAG 1 cut(s) 153
SfoI GGCGCC 1 cut(s) 433
SmiMI CAYNNNNRTG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 245
SmoI CTYRAG 1 cut(s) 245
SnaBI TACGTA 1 cut(s) 410
SseBI AGGCCT 1 cut(s) 503
SsiI CCGC 2 cut(s) 255, 314
SspDI GGCGCC 1 cut(s) 431
SspMI CTAG 2 cut(s) 192, 520
StuI AGGCCT 1 cut(s) 503
StyD4I CCNGG 1 cut(s) 250
TaaI ACNGT 1 cut(s) 131
TaiI ACGT 1 cut(s) 412
TatI WGTACW 1 cut(s) 363
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TscAI CASTG 3 cut(s) 94, 115, 192
TseI GCWGC 3 cut(s) 68, 425, 494
TspDTI ATGAA 2 cut(s) 274, 367
TspRI CASTG 3 cut(s) 94, 115, 192
XspI CTAG 2 cut(s) 192, 520
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.