MD11G1241500.v1.1

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0014323
Physical Location & Seq
Forward (+)
35144633 .. 35147075
2443 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1241500.v1.1.491

Sequence Viewer

Length: 555 bp
ATGGCATCCAAGCTTCTACTGATTACGGTCTTCATCCTCAATGTCATTGCTTTTGGGCTTGCTGTTGCAGCTGAGCAGAGAAGAAGCTCTGCAAAGGTCGTTCAGGATACTGACGTGAATTACAACTACTGTGTCTATGAGTCAGACATAGCAACTGGCTATGGGGTTGGTGCATTTTTGTTCTTCTTGGTAGGTCAGGTCATTATAATGGTGGCAAGCCGATGCTTCTGTTGCGGGAAGCCATTGAGGCCTGGAGGGTCAAGAGCTTTGGCAGTTATCCTTTTCATAATCTGCAGGGTATTGTTCTTAATCGCTGAGGTGTGCTTGTTGGCGGGTTCGGTGAGGAATGCATACCACACCAAGTACAGGACCATTTTCAGCGAAAGCCCTCCAGATTGCCAGCAACTAAGAAAGGGAGTTTTCGGTGCAGGGGCAGCTTTCATTTTCCTCACCACAATTGTTTCTGAGTTCTACTATGTTTGCTATTCCAGAGCCAGGGAAAGCTTCCAGCCATACGGGGGAGAGGCTGCAGTTGGTCTCGGAACCTTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

185

Amino Acids

20.11

Weight (kDa)

8.94

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 153 2.3e-29 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014323)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27435
fragaria_vesca FvH4_3g20620
malus_domestica MD03G1223600.v1.1 MD11G1241500.v1.1
prunus_persica Prupe.4G185900_v2.0.a1
pyrus_communis pycom03g17190 pycom11g21310
rosa_chinensis RchiOBHm_Chr5g0034641
rosa_laevigata RLG00000033562
rosa_multiflora Rmu_sc0002460.1_g000087
rosa_roxburghii Rroxscaffold_1G00045680
rosa_rugosa Rorug05G0147400 Rorug05G0147400
rosa_samantha Rh5AG239400 Rh5BG240100 Rh5DG248300
rosa_wichuraiana Rw5G021940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 206
AciI CCGC 2 cut(s) 234, 332
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 3 cut(s) 366, 495, 518
AgsI TTSAA 1 cut(s) 550
AjiI CACGTC 1 cut(s) 115
AjnI CCWGG 2 cut(s) 250, 494
AluBI AGCT 6 cut(s) 13, 71, 87, 266, 437, 504
AluI AGCT 6 cut(s) 13, 71, 87, 266, 437, 504
Alw26I GTCTC 1 cut(s) 542
AoxI GGCC 1 cut(s) 248
ApeKI GCWGC 3 cut(s) 68, 434, 527
AspS9I GGNCC 1 cut(s) 369
AsuHPI GGTGA 2 cut(s) 352, 442
AvaII GGWCC 1 cut(s) 369
BaeI ACNNNNGTAYC 2 cut(s) 99, 132
BbsI GAAGAC 1 cut(s) 22
BbvCI CCTCAGC 1 cut(s) 315
BbvI GCAGC 3 cut(s) 80, 446, 514
BciT130I CCWGG 2 cut(s) 252, 496
BciVI GTATCC 1 cut(s) 100
BcoDI GTCTC 1 cut(s) 542
BfmI CTRYAG 2 cut(s) 292, 528
BfuI GTATCC 1 cut(s) 100
BglI GCCNNNNNGGC 1 cut(s) 247
BisI GCNGC 3 cut(s) 69, 435, 528
BlpI GCTNAGC 1 cut(s) 72
BlsI GCNGC 3 cut(s) 70, 436, 529
Bme1390I CCNGG 2 cut(s) 252, 496
Bme18I GGWCC 1 cut(s) 369
BmgBI CACGTC 1 cut(s) 115
BmgT120I GGNCC 1 cut(s) 369
BmiI GGNNCC 1 cut(s) 544
BmrFI CCNGG 2 cut(s) 252, 496
BmsI GCATC 2 cut(s) 14, 212
BpiI GAAGAC 1 cut(s) 22
BpmI CTGGAG 2 cut(s) 273, 375
Bpu10I CCTNAGC 1 cut(s) 315
Bpu1102I GCTNAGC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 542
BsaJI CCNNGG 1 cut(s) 495
Bsc4I CCNNNNNNNGG 3 cut(s) 366, 495, 518
Bse1I ACTGG 1 cut(s) 160
Bse3DI GCAATG 1 cut(s) 45
BseBI CCWGG 2 cut(s) 252, 496
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 2 cut(s) 5, 33
BseLI CCNNNNNNNGG 3 cut(s) 366, 495, 518
BseMI GCAATG 1 cut(s) 45
BseMII CTCAG 3 cut(s) 63, 306, 456
BseNI ACTGG 1 cut(s) 160
BseXI GCAGC 3 cut(s) 80, 446, 514
BsgI GTGCAG 1 cut(s) 447
BshFI GGCC 1 cut(s) 250
BslI CCNNNNNNNGG 3 cut(s) 366, 495, 518
BsmAI GTCTC 1 cut(s) 542
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 1 cut(s) 250
Bso31I GGTCTC 1 cut(s) 542
Bsp1720I GCTNAGC 1 cut(s) 72
BspACI CCGC 2 cut(s) 234, 332
BspANI GGCC 1 cut(s) 250
BspCNI CTCAG 3 cut(s) 64, 307, 457
BspLI GGNNCC 1 cut(s) 544
BspMAI CTGCAG 2 cut(s) 296, 532
BspTNI GGTCTC 1 cut(s) 542
BsrDI GCAATG 1 cut(s) 45
BsrI ACTGG 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 495
Bst2UI CCWGG 2 cut(s) 252, 496
Bst4CI ACNGT 2 cut(s) 28, 131
BstC8I GCNNGC 3 cut(s) 60, 217, 401
BstDEI CTNAG 4 cut(s) 72, 315, 407, 465
BstF5I GGATG 2 cut(s) 5, 33
BstMAI GTCTC 1 cut(s) 542
BstMWI GCNNNNNNNGC 4 cut(s) 68, 231, 247, 434
BstNI CCWGG 2 cut(s) 252, 496
BstSCI CCNGG 2 cut(s) 250, 494
BstSFI CTRYAG 2 cut(s) 292, 528
BstV1I GCAGC 3 cut(s) 80, 446, 514
BstV2I GAAGAC 1 cut(s) 22
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 1 cut(s) 250
BtrI CACGTC 1 cut(s) 115
BtsCI GGATG 2 cut(s) 5, 33
Cac8I GCNNGC 3 cut(s) 60, 217, 401
Cfr13I GGNCC 1 cut(s) 369
Csp6I GTAC 1 cut(s) 364
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 4 cut(s) 72, 315, 407, 465
Eco147I AGGCCT 1 cut(s) 250
Eco31I GGTCTC 1 cut(s) 542
Eco47I GGWCC 1 cut(s) 369
EcoRII CCWGG 2 cut(s) 250, 494
EcoT22I ATGCAT 1 cut(s) 352
FaiI YATR 8 cut(s) 138, 149, 162, 206, 287, 352, 477, 514
FauI CCCGC 2 cut(s) 227, 325
Fnu4HI GCNGC 3 cut(s) 69, 435, 528
FokI GGATG 1 cut(s) 20
Fsp4HI GCNGC 3 cut(s) 69, 435, 528
GluI GCNGC 3 cut(s) 69, 435, 528
GsuI CTGGAG 2 cut(s) 273, 375
HaeIII GGCC 1 cut(s) 250
HindIII AAGCTT 2 cut(s) 11, 502
HinfI GANTC 1 cut(s) 140
HphI GGTGA 2 cut(s) 352, 442
Hpy188I TCNGA 3 cut(s) 145, 466, 542
Hpy188III TCNNGA 4 cut(s) 104, 261, 392, 489
HpyCH4III ACNGT 2 cut(s) 28, 131
HpyCH4IV ACGT 1 cut(s) 114
HpyCH4V TGCA 7 cut(s) 68, 92, 173, 294, 350, 428, 530
HpyF10VI GCNNNNNNNGC 4 cut(s) 68, 231, 247, 434
HpyF3I CTNAG 4 cut(s) 72, 315, 407, 465
HpySE526I ACGT 1 cut(s) 114
Lsp1109I GCAGC 3 cut(s) 80, 446, 514
LweI GCATC 2 cut(s) 14, 212
MaeII ACGT 1 cut(s) 114
MboII GAAGA 3 cut(s) 22, 93, 175
MfeI CAATTG 1 cut(s) 456
MluCI AATT 2 cut(s) 118, 456
MlyI GAGTC 1 cut(s) 149
MnlI CCTC 8 cut(s) 47, 240, 248, 310, 336, 399, 458, 517
Mph1103I ATGCAT 1 cut(s) 352
MseI TTAA 1 cut(s) 308
MslI CAYNNNNRTG 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 71
MspR9I CCNGG 2 cut(s) 252, 496
MunI CAATTG 1 cut(s) 456
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 2 cut(s) 252, 496
MwoI GCNNNNNNNGC 4 cut(s) 68, 231, 247, 434
NlaIV GGNNCC 1 cut(s) 544
NsiI ATGCAT 1 cut(s) 352
PceI AGGCCT 1 cut(s) 250
PctI GAATGC 1 cut(s) 352
PkrI GCNGC 3 cut(s) 70, 436, 529
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PsiI TTATAA 1 cut(s) 206
Psp6I CCWGG 2 cut(s) 250, 494
PspGI CCWGG 2 cut(s) 250, 494
PspN4I GGNNCC 1 cut(s) 544
PspPI GGNCC 1 cut(s) 369
PstI CTGCAG 2 cut(s) 296, 532
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 1 cut(s) 206
SaqAI TTAA 1 cut(s) 308
SatI GCNGC 3 cut(s) 69, 435, 528
Sau96I GGNCC 1 cut(s) 369
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 2 cut(s) 252, 496
SfaNI GCATC 2 cut(s) 14, 212
SfcI CTRYAG 2 cut(s) 292, 528
SinI GGWCC 1 cut(s) 369
SmiMI CAYNNNNRTG 1 cut(s) 206
Sse9I AATT 2 cut(s) 118, 456
SseBI AGGCCT 1 cut(s) 250
SsiI CCGC 2 cut(s) 234, 332
StuI AGGCCT 1 cut(s) 250
StyD4I CCNGG 2 cut(s) 250, 494
TaaI ACNGT 2 cut(s) 28, 131
TaiI ACGT 1 cut(s) 117
TasI AATT 2 cut(s) 118, 456
TatI WGTACW 1 cut(s) 363
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TseI GCWGC 3 cut(s) 68, 434, 527
TspDTI ATGAA 3 cut(s) 22, 274, 430
VpaK11BI GGWCC 1 cut(s) 369
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.