Rorug05G0147400

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000710
Physical Location & Seq
Forward (+)
13719925 .. 13724311
4387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0147400.1

Sequence Viewer

Length: 498 bp
ATGGAGAAGGGTATCCAAGGGACCATAAATCTGAGAAGAAATGGAGAAGAAGAAGAAGAAACTGTGGATTTGAGCGGTAAGGTGCACCTGCTCCCCTGCTGCATCAAGTACAATGGCCCTTGTGACGTTTCGGACTACTTCAAACCCAAACCCACTGGAATGGAGTCCGAGAGCTTAAAGACGCAGGAGGCTTGTTTCCGAGGAAGGAAGTTGCTGGGAGCATCCGTTCCCATCCCAGATGGGTATTCTGGATTTGTCCTTGGGAAGAAGAGTCTTGACAAGAGAAAAGCTTCAAATATATCTGAAGGGAATTCAAACTGTTGGGAGATAAATGCCAAATATAAAAACATTACATATTGGAATCATGACAGCCTTCCTTCGCAGGATGATGCATTCTTGCGTTCTTTTCATTGGCTTGCTGTAGCAAAAACTATGCATGAACCAGTAGCAGCAGAAGATTTGGTTTCTGCATCCGCTGTTCTGGAAAAGTTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

165

Amino Acids

18.35

Weight (kDa)

6.1

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNase_H2_suC PF08615 28 - 145 4.8e-28 Ribonuclease H2 non-catalytic subunit (Ylr154p-like)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 96
Acc36I ACCTGC 1 cut(s) 96
AccBSI CCGCTC 1 cut(s) 75
AciI CCGC 2 cut(s) 75, 474
AcsI RAATTY 1 cut(s) 310
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 1 cut(s) 110
AgsI TTSAA 4 cut(s) 142, 294, 315, 493
AloI GAACNNNNNNTCC 2 cut(s) 210, 242
AluBI AGCT 2 cut(s) 174, 290
AluI AGCT 2 cut(s) 174, 290
Alw21I GWGCWC 1 cut(s) 87
Alw44I GTGCAC 1 cut(s) 83
AoxI GGCC 1 cut(s) 115
ApaLI GTGCAC 1 cut(s) 83
ApeKI GCWGC 2 cut(s) 99, 449
ApoI RAATTY 1 cut(s) 310
Asp700I GAANNNNTTC 1 cut(s) 289
AspS9I GGNCC 2 cut(s) 21, 116
AvaII GGWCC 1 cut(s) 21
BaeGI GKGCMC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 87
BbvI GCAGC 2 cut(s) 86, 461
BccI CCATC 2 cut(s) 233, 239
BciVI GTATCC 1 cut(s) 23
BfmI CTRYAG 1 cut(s) 420
BfuAI ACCTGC 1 cut(s) 96
BfuI GTATCC 1 cut(s) 23
BisI GCNGC 2 cut(s) 100, 450
BlsI GCNGC 2 cut(s) 101, 451
Bme18I GGWCC 1 cut(s) 21
BmgT120I GGNCC 2 cut(s) 21, 116
BmiI GGNNCC 1 cut(s) 22
BmsI GCATC 4 cut(s) 111, 230, 379, 479
BsaJI CCNNGG 3 cut(s) 16, 199, 259
BsaXI ACNNNNNCTCC 2 cut(s) 210, 240
Bse1I ACTGG 2 cut(s) 160, 443
BseDI CCNNGG 3 cut(s) 16, 199, 259
BseGI GGATG 4 cut(s) 221, 231, 391, 470
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 2 cut(s) 160, 443
BseSI GKGCMC 1 cut(s) 87
BseXI GCAGC 2 cut(s) 86, 461
BseYI CCCAGC 1 cut(s) 214
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 87
BslFI GGGAC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 34
BsmI GAATGC 1 cut(s) 392
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 1 cut(s) 87
BspACI CCGC 2 cut(s) 75, 474
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 1 cut(s) 24
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 96
BsrBI CCGCTC 1 cut(s) 75
BsrI ACTGG 2 cut(s) 160, 443
BssECI CCNNGG 3 cut(s) 16, 199, 259
BssT1I CCWWGG 2 cut(s) 16, 259
Bst4CI ACNGT 2 cut(s) 64, 320
Bst6I CTCTTC 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 417
BstDEI CTNAG 1 cut(s) 32
BstF5I GGATG 4 cut(s) 221, 231, 391, 470
BstSFI CTRYAG 1 cut(s) 420
BstSLI GKGCMC 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 86, 461
BstXI CCANNNNNNTGG 1 cut(s) 160
BsuI GTATCC 1 cut(s) 23
BsuRI GGCC 1 cut(s) 117
BtsCI GGATG 4 cut(s) 221, 231, 391, 470
BtsIMutI CAGTG 1 cut(s) 153
BveI ACCTGC 1 cut(s) 96
Cac8I GCNNGC 1 cut(s) 417
CciI TCATGA 1 cut(s) 364
Cfr13I GGNCC 2 cut(s) 21, 116
CseI GACGC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 109
CviAII CATG 2 cut(s) 365, 437
CviJI RGCY 6 cut(s) 117, 174, 191, 290, 372, 415
CviKI_1 RGCY 6 cut(s) 117, 174, 191, 290, 372, 415
CviQI GTAC 1 cut(s) 109
DdeI CTNAG 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 263
EarI CTCTTC 1 cut(s) 263
Eco130I CCWWGG 2 cut(s) 16, 259
Eco47I GGWCC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 324
EcoRI GAATTC 1 cut(s) 310
EcoT14I CCWWGG 2 cut(s) 16, 259
EcoT22I ATGCAT 2 cut(s) 394, 438
ErhI CCWWGG 2 cut(s) 16, 259
FaeI CATG 2 cut(s) 368, 440
FaiI YATR 7 cut(s) 26, 299, 342, 355, 366, 434, 438
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 364, 436
Fnu4HI GCNGC 2 cut(s) 100, 450
FokI GGATG 4 cut(s) 208, 218, 398, 457
Fsp4HI GCNGC 2 cut(s) 100, 450
GluI GCNGC 2 cut(s) 100, 450
GsaI CCCAGC 1 cut(s) 218
HaeIII GGCC 1 cut(s) 117
HgaI GACGC 1 cut(s) 190
Hin1II CATG 2 cut(s) 368, 440
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 3 cut(s) 164, 271, 361
Hpy166II GTNNAC 1 cut(s) 85
Hpy188I TCNGA 5 cut(s) 33, 133, 169, 200, 304
Hpy188III TCNNGA 4 cut(s) 249, 275, 365, 482
Hpy8I GTNNAC 1 cut(s) 85
HpyAV CCTTC 4 cut(s) 198, 299, 383, 387
HpyCH4III ACNGT 2 cut(s) 64, 320
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 5 cut(s) 85, 102, 392, 436, 470
HpyF3I CTNAG 1 cut(s) 32
HpySE526I ACGT 1 cut(s) 126
Hsp92II CATG 2 cut(s) 368, 440
LmnI GCTCC 2 cut(s) 96, 218
Lsp1109I GCAGC 2 cut(s) 86, 461
LweI GCATC 4 cut(s) 111, 230, 379, 479
MaeII ACGT 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 122
MbiI CCGCTC 1 cut(s) 75
MboII GAAGA 8 cut(s) 48, 59, 62, 65, 68, 277, 280, 467
MhlI GDGCHC 1 cut(s) 87
MluCI AATT 1 cut(s) 310
MlyI GAGTC 2 cut(s) 173, 280
MnlI CCTC 2 cut(s) 181, 194
Mph1103I ATGCAT 2 cut(s) 394, 438
MroXI GAANNNNTTC 1 cut(s) 289
MseI TTAA 1 cut(s) 176
MslI CAYNNNNRTG 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 476
Mva1269I GAATGC 1 cut(s) 392
NlaIII CATG 2 cut(s) 368, 440
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 1 cut(s) 122
NsiI ATGCAT 2 cut(s) 394, 438
PagI TCATGA 1 cut(s) 364
PaqCI CACCTGC 1 cut(s) 96
PctI GAATGC 1 cut(s) 392
PdmI GAANNNNTTC 1 cut(s) 289
PfeI GAWTC 1 cut(s) 361
PkrI GCNGC 2 cut(s) 101, 451
PleI GAGTC 2 cut(s) 172, 279
PpsI GAGTC 2 cut(s) 172, 279
PspFI CCCAGC 1 cut(s) 214
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 2 cut(s) 21, 116
RsaI GTAC 1 cut(s) 110
RsaNI GTAC 1 cut(s) 109
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 1 cut(s) 176
SatI GCNGC 2 cut(s) 100, 450
Sau96I GGNCC 2 cut(s) 21, 116
SchI GAGTC 2 cut(s) 173, 280
SduI GDGCHC 1 cut(s) 87
SetI ASST 5 cut(s) 84, 90, 129, 176, 292
SfaNI GCATC 4 cut(s) 111, 230, 379, 479
SfcI CTRYAG 1 cut(s) 420
SinI GGWCC 1 cut(s) 21
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 1 cut(s) 310
SsiI CCGC 2 cut(s) 75, 474
StyI CCWWGG 2 cut(s) 16, 259
TaaI ACNGT 2 cut(s) 64, 320
TaiI ACGT 1 cut(s) 129
TasI AATT 1 cut(s) 310
TatI WGTACW 1 cut(s) 108
TfiI GAWTC 1 cut(s) 361
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TscAI CASTG 1 cut(s) 160
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 2 cut(s) 99, 449
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 2 cut(s) 398, 453
TspGWI ACGGA 1 cut(s) 214
TspRI CASTG 1 cut(s) 160
VneI GTGCAC 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 21
XapI RAATTY 1 cut(s) 310
XmnI GAANNNNTTC 1 cut(s) 289
Zsp2I ATGCAT 2 cut(s) 394, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.