FvH4_4g27942

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000710
Physical Location & Seq
Reverse (-)
28731998 .. 28733455
1458 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27942.t1

Sequence Viewer

Length: 909 bp
ATGGACCACTCTAACTGGTGGGTTCAAATAGTGAAGAATAAGTATTTAAAGAACCATTCCTTCTTTGAAACAAAAAAGAAAAGCAGTGACTCCATTGCTTGGAAAGGCATCCTGGATAGCAGAGATCTCATTACAAAAGGGATGAGATGGACTATTGGCACAGGAAAGGAGGTTAAATTCTGGACTTTTAATTGGATTTTTGATTTTCCTCTTAACAATTTGTTAAACGATCAGCAGAAAGCTACTATTAATTGGGATGCAAATGCCAGGAACGATGCTTCTTTCAGAAACATTAGTACCTCCCCTCTAACCATTATTAGTGTTGCTGCTTCTATAGAGAAAAGCTTCAAGGAAAATAACATGGATTGCAGGGTTGGTTCTTTCAAAACACCATCAGCCATTAAATGGCAACCACCTCCTGCATCCACTTTCAAAGTAAACTTTGATGGCTCGGTGACTTCCTCTTCTGCAGCCTGTGGTACTATCATCAGGAATTCTGAAGGTGTACCAATTATTACTTCATCAAAGAACATTGGGAAGGCCTCGGTTCCCACAACTGAAGCGACGGCACTAAGAGACAGCATCCTCATTGCCAAGAACAGGGGGCTGACAAGGATCATTGTAGAGGGTGACTCCAAAATCATTATTGATGCAGTGAATGGCAAAATTAGCACCCCATGGAGGCTCCAACAGATTACAGAAGACATTAAAAACTTGGCTCAAGAGTTCCAGGAGATTAGCTTTAACCACATATACAGGGAAGCAAATTTTGCAGCAGACGCCACAGCAAGTTTGGGACATTCAATAACAGGAAGAAGAGACTGGTTCCATCCTTTGCCCAGGTCTATTGGAAGAATGATCCTTTTTGACCAAATTGGTGAAGGCTGCCCAAGAGGCAGTTTCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

303

Amino Acids

33.82

Weight (kDa)

9.49

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 147 - 264 2.3e-23 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 99, 405
AclWI GGATC 2 cut(s) 623, 853
AcsI RAATTY 3 cut(s) 176, 493, 766
AcuI CTGAAG 2 cut(s) 519, 579
AcyI GRCGYC 1 cut(s) 780
AfaI GTAC 3 cut(s) 298, 481, 507
AfiI CCNNNNNNNGG 4 cut(s) 99, 405, 600, 681
AgsI TTSAA 6 cut(s) 26, 68, 349, 385, 433, 804
AjnI CCWGG 4 cut(s) 111, 266, 729, 839
AjuI GAANNNNNNNTTGG 2 cut(s) 502, 534
AluBI AGCT 3 cut(s) 242, 345, 741
AluI AGCT 3 cut(s) 242, 345, 741
Alw26I GTCTC 2 cut(s) 570, 813
AlwI GGATC 2 cut(s) 623, 853
AoxI GGCC 1 cut(s) 540
ApeKI GCWGC 4 cut(s) 326, 470, 773, 885
ApoI RAATTY 3 cut(s) 176, 493, 766
AseI ATTAAT 1 cut(s) 249
Asp700I GAANNNNTTC 1 cut(s) 344
AspS9I GGNCC 1 cut(s) 4
AsuHPI GGTGA 3 cut(s) 466, 641, 890
AvaII GGWCC 1 cut(s) 4
BaeI ACNNNNGTAYC 2 cut(s) 280, 313
BbsI GAAGAC 1 cut(s) 708
BbvI GCAGC 4 cut(s) 313, 482, 785, 872
BccI CCATC 4 cut(s) 141, 400, 440, 837
BceAI ACGGC 1 cut(s) 582
BciT130I CCWGG 4 cut(s) 113, 268, 731, 841
BcoDI GTCTC 2 cut(s) 570, 813
BfaI CTAG 1 cut(s) 907
BfmI CTRYAG 2 cut(s) 333, 468
BglI GCCNNNNNGGC 1 cut(s) 894
BglII AGATCT 1 cut(s) 124
BisI GCNGC 4 cut(s) 327, 471, 774, 886
BlsI GCNGC 4 cut(s) 328, 472, 775, 887
Bme1390I CCNGG 4 cut(s) 113, 268, 731, 841
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 3 cut(s) 549, 686, 827
BmrFI CCNGG 4 cut(s) 113, 268, 731, 841
BmsI GCATC 6 cut(s) 117, 247, 265, 431, 591, 640
BpiI GAAGAC 1 cut(s) 708
BplI GAGNNNNNCTC 2 cut(s) 617, 649
BpuEI CTTGAG 1 cut(s) 705
BsaHI GRCGYC 1 cut(s) 780
BsaJI CCNNGG 3 cut(s) 543, 677, 839
Bsc4I CCNNNNNNNGG 4 cut(s) 99, 405, 600, 681
Bse1I ACTGG 2 cut(s) 20, 827
Bse3DI GCAATG 2 cut(s) 93, 588
BseBI CCWGG 4 cut(s) 113, 268, 731, 841
BseDI CCNNGG 3 cut(s) 543, 677, 839
BseGI GGATG 6 cut(s) 108, 147, 262, 422, 582, 829
BseLI CCNNNNNNNGG 4 cut(s) 99, 405, 600, 681
BseMI GCAATG 2 cut(s) 93, 588
BseNI ACTGG 2 cut(s) 20, 827
BseXI GCAGC 4 cut(s) 313, 482, 785, 872
BshFI GGCC 1 cut(s) 542
BslFI GGGAC 1 cut(s) 810
BslI CCNNNNNNNGG 4 cut(s) 99, 405, 600, 681
BsmAI GTCTC 2 cut(s) 570, 813
BsmFI GGGAC 1 cut(s) 810
BsnI GGCC 1 cut(s) 542
Bsp143I GATC 4 cut(s) 124, 229, 615, 858
Bsp19I CCATGG 1 cut(s) 677
BspANI GGCC 1 cut(s) 542
BspLI GGNNCC 3 cut(s) 549, 686, 827
BspMAI CTGCAG 1 cut(s) 472
BspPI GGATC 2 cut(s) 623, 853
BsrDI GCAATG 2 cut(s) 93, 588
BsrI ACTGG 2 cut(s) 20, 827
BssECI CCNNGG 3 cut(s) 543, 677, 839
BssMI GATC 4 cut(s) 124, 229, 615, 858
BssNI GRCGYC 1 cut(s) 780
BssT1I CCWWGG 1 cut(s) 677
Bst2UI CCWGG 4 cut(s) 113, 268, 731, 841
Bst6I CTCTTC 2 cut(s) 469, 811
BstACI GRCGYC 1 cut(s) 780
BstAPI GCANNNNNTGC 1 cut(s) 770
BstDEI CTNAG 1 cut(s) 572
BstDSI CCRYGG 1 cut(s) 677
BstF5I GGATG 6 cut(s) 108, 147, 262, 422, 582, 829
BstKTI GATC 4 cut(s) 127, 232, 618, 861
BstMAI GTCTC 2 cut(s) 570, 813
BstMBI GATC 4 cut(s) 124, 229, 615, 858
BstMWI GCNNNNNNNGC 4 cut(s) 669, 770, 779, 894
BstNI CCWGG 4 cut(s) 113, 268, 731, 841
BstSCI CCNGG 4 cut(s) 111, 266, 729, 839
BstSFI CTRYAG 2 cut(s) 333, 468
BstV1I GCAGC 4 cut(s) 313, 482, 785, 872
BstV2I GAAGAC 1 cut(s) 708
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BsuRI GGCC 1 cut(s) 542
BtgI CCRYGG 1 cut(s) 677
BtsCI GGATG 6 cut(s) 108, 147, 262, 422, 582, 829
BtsI GCAGTG 2 cut(s) 91, 660
BtsIMutI CAGTG 2 cut(s) 91, 660
Cfr13I GGNCC 1 cut(s) 4
CseI GACGC 1 cut(s) 788
Csp6I GTAC 3 cut(s) 297, 480, 506
CviAII CATG 2 cut(s) 361, 678
CviQI GTAC 3 cut(s) 297, 480, 506
DdeI CTNAG 1 cut(s) 572
DpnI GATC 4 cut(s) 126, 231, 617, 860
DpnII GATC 4 cut(s) 124, 229, 615, 858
DraI TTTAAA 1 cut(s) 48
Eam1104I CTCTTC 2 cut(s) 469, 811
EarI CTCTTC 2 cut(s) 469, 811
Eco130I CCWWGG 1 cut(s) 677
Eco147I AGGCCT 1 cut(s) 542
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 2 cut(s) 519, 579
EcoRI GAATTC 1 cut(s) 493
EcoRII CCWGG 4 cut(s) 111, 266, 729, 839
EcoT14I CCWWGG 1 cut(s) 677
ErhI CCWWGG 1 cut(s) 677
FaeI CATG 2 cut(s) 364, 681
FaiI YATR 5 cut(s) 335, 362, 679, 752, 754
FaqI GGGAC 1 cut(s) 810
FatI CATG 2 cut(s) 360, 677
Fnu4HI GCNGC 4 cut(s) 327, 471, 774, 886
FokI GGATG 6 cut(s) 95, 154, 269, 409, 569, 816
Fsp4HI GCNGC 4 cut(s) 327, 471, 774, 886
FspBI CTAG 1 cut(s) 907
GluI GCNGC 4 cut(s) 327, 471, 774, 886
HaeIII GGCC 1 cut(s) 542
HgaI GACGC 1 cut(s) 788
Hin1I GRCGYC 1 cut(s) 780
Hin1II CATG 2 cut(s) 364, 681
HindIII AAGCTT 1 cut(s) 343
HinfI GANTC 2 cut(s) 89, 632
HphI GGTGA 3 cut(s) 466, 641, 890
Hpy166II GTNNAC 2 cut(s) 439, 506
Hpy188I TCNGA 2 cut(s) 287, 499
Hpy188III TCNNGA 3 cut(s) 181, 490, 722
Hpy8I GTNNAC 2 cut(s) 439, 506
Hpy99I CGWCG 1 cut(s) 568
HpyAV CCTTC 4 cut(s) 70, 494, 532, 875
HpyCH4V TGCA 6 cut(s) 260, 369, 422, 470, 653, 773
HpyF10VI GCNNNNNNNGC 4 cut(s) 669, 770, 779, 894
HpyF3I CTNAG 1 cut(s) 572
Hsp92I GRCGYC 1 cut(s) 780
Hsp92II CATG 2 cut(s) 364, 681
Kzo9I GATC 4 cut(s) 124, 229, 615, 858
LmnI GCTCC 1 cut(s) 690
Lsp1109I GCAGC 4 cut(s) 313, 482, 785, 872
LweI GCATC 6 cut(s) 117, 247, 265, 431, 591, 640
MaeI CTAG 1 cut(s) 907
MaeIII GTNAC 3 cut(s) 86, 454, 629
MalI GATC 4 cut(s) 126, 231, 617, 860
MboI GATC 4 cut(s) 124, 229, 615, 858
MboII GAAGA 6 cut(s) 46, 456, 713, 825, 828, 864
MflI RGATCY 1 cut(s) 124
MluCI AATT 9 cut(s) 176, 190, 217, 250, 493, 510, 666, 766, 873
MlyI GAGTC 2 cut(s) 83, 626
MmeI TCCRAC 1 cut(s) 712
MroXI GAANNNNTTC 1 cut(s) 344
MseI TTAA 9 cut(s) 47, 174, 189, 213, 224, 249, 402, 708, 744
MspR9I CCNGG 4 cut(s) 113, 268, 731, 841
MvaI CCWGG 4 cut(s) 113, 268, 731, 841
MwoI GCNNNNNNNGC 4 cut(s) 669, 770, 779, 894
NcoI CCATGG 1 cut(s) 677
NdeII GATC 4 cut(s) 124, 229, 615, 858
NlaIII CATG 2 cut(s) 364, 681
NlaIV GGNNCC 3 cut(s) 549, 686, 827
NmuCI GTSAC 3 cut(s) 86, 454, 629
PceI AGGCCT 1 cut(s) 542
PdmI GAANNNNTTC 1 cut(s) 344
PflMI CCANNNNNTGG 2 cut(s) 99, 405
PfoI TCCNGGA 2 cut(s) 111, 729
PkrI GCNGC 4 cut(s) 328, 472, 775, 887
PleI GAGTC 2 cut(s) 83, 626
PpsI GAGTC 2 cut(s) 83, 626
PshBI ATTAAT 1 cut(s) 249
Psp6I CCWGG 4 cut(s) 111, 266, 729, 839
PspGI CCWGG 4 cut(s) 111, 266, 729, 839
PspN4I GGNNCC 3 cut(s) 549, 686, 827
PspPI GGNCC 1 cut(s) 4
PstI CTGCAG 1 cut(s) 472
PsuI RGATCY 1 cut(s) 124
RsaI GTAC 3 cut(s) 298, 481, 507
RsaNI GTAC 3 cut(s) 297, 480, 506
SaqAI TTAA 9 cut(s) 47, 174, 189, 213, 224, 249, 402, 708, 744
SatI GCNGC 4 cut(s) 327, 471, 774, 886
Sau3AI GATC 4 cut(s) 124, 229, 615, 858
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 2 cut(s) 83, 626
ScrFI CCNGG 4 cut(s) 113, 268, 731, 841
SetI ASST 8 cut(s) 174, 244, 302, 347, 418, 505, 743, 845
SfaNI GCATC 6 cut(s) 117, 247, 265, 431, 591, 640
SfcI CTRYAG 2 cut(s) 333, 468
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 1 cut(s) 720
SmoI CTYRAG 1 cut(s) 720
Sse9I AATT 9 cut(s) 176, 190, 217, 250, 493, 510, 666, 766, 873
SseBI AGGCCT 1 cut(s) 542
SspMI CTAG 1 cut(s) 907
StuI AGGCCT 1 cut(s) 542
StyD4I CCNGG 4 cut(s) 111, 266, 729, 839
StyI CCWWGG 1 cut(s) 677
TasI AATT 9 cut(s) 176, 190, 217, 250, 493, 510, 666, 766, 873
Tru1I TTAA 9 cut(s) 47, 174, 189, 213, 224, 249, 402, 708, 744
Tru9I TTAA 9 cut(s) 47, 174, 189, 213, 224, 249, 402, 708, 744
TscAI CASTG 2 cut(s) 91, 660
TseFI GTSAC 3 cut(s) 86, 454, 629
TseI GCWGC 4 cut(s) 326, 470, 773, 885
Tsp45I GTSAC 3 cut(s) 86, 454, 629
TspDTI ATGAA 1 cut(s) 510
TspRI CASTG 2 cut(s) 91, 660
Van91I CCANNNNNTGG 2 cut(s) 99, 405
VpaK11BI GGWCC 1 cut(s) 4
VspI ATTAAT 1 cut(s) 249
XapI RAATTY 3 cut(s) 176, 493, 766
XcmI CCANNNNNNNNNTGG 1 cut(s) 790
XmnI GAANNNNTTC 1 cut(s) 344
XspI CTAG 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.