Rw6G040600

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000710
Physical Location & Seq
Forward (+)
64347656 .. 64348261
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G040600.1

Sequence Viewer

Length: 606 bp
ATGTCAATTGTTATGTTAGTTGAAGCAATAATGAAAAGCTTTGCTGATGTTAACACTAGTACTAGTGGAGCAAAAACTTCAAACCTTTCAACCACAATTAAGTGGTATGTTCCTCCTAATTCTTTTGTTAAAATAAATTTTGATGGCTCAGTTAGAAGGGATTCAGCTGCTAGTGGTTTTGTTATTAGAGACCATAATGGAATACTAGTTATTGCTGCAACCAAGTGTGTTGGCAATTCTTCTACTTCTTTTGTAGAAGCTACTGCACTTAGGGATAGTTTAATTGCGGCCAAAGACAAAGGTTTCACAAGAGTTGAAGTTGAAGGAGACTCAAAGCTGGTTATTGATGCAGTTACAGGAATGGTAATTCCACCATGGAGGCTACTCAAATTGATTGAAGACATTAGAACCATTGCAAATTCCTTTTCTCAAATTACCTTTAAGCATATTTGTAGAGAAGCAAACTTTGTAGCTGATGCTATTGCAAATTTAAGACATAATGCTGCTTCTCCTATGTCTTGGAGTGATAGGGTCCCAAGTGAGGGTTATAGAGCCTTAATTTTTGACGTTGTAAACTTTGGTTGCCCAAGAGACTTTCTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

201

Amino Acids

21.98

Weight (kDa)

8.48

Isoelectric Point (pI)

29.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 46 - 163 1.3e-24 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 287
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 3 cut(s) 136, 418, 487
AfaI GTAC 1 cut(s) 61
AfiI CCNNNNNNNGG 2 cut(s) 541, 542
AgsI TTSAA 6 cut(s) 23, 81, 90, 317, 323, 398
AhlI ACTAGT 3 cut(s) 56, 62, 205
AluBI AGCT 5 cut(s) 39, 167, 260, 337, 473
AluI AGCT 5 cut(s) 39, 167, 260, 337, 473
Alw26I GTCTC 3 cut(s) 183, 321, 585
AoxI GGCC 1 cut(s) 288
ApeKI GCWGC 3 cut(s) 167, 215, 503
ApoI RAATTY 3 cut(s) 136, 418, 487
Asp700I GAANNNNTTC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 532
AvaII GGWCC 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 405
BbvI GCAGC 3 cut(s) 154, 202, 490
BccI CCATC 1 cut(s) 137
BcoDI GTCTC 3 cut(s) 183, 321, 585
BcuI ACTAGT 3 cut(s) 56, 62, 205
BfaI CTAG 4 cut(s) 57, 63, 171, 206
BisI GCNGC 4 cut(s) 168, 216, 288, 504
BlsI GCNGC 4 cut(s) 169, 217, 289, 505
BmcAI AGTACT 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 532
BmgT120I GGNCC 1 cut(s) 532
BmiI GGNNCC 2 cut(s) 533, 534
BmsI GCATC 2 cut(s) 337, 466
BpiI GAAGAC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 1 cut(s) 374
Bsc4I CCNNNNNNNGG 2 cut(s) 541, 542
Bse3DI GCAATG 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 374
BseLI CCNNNNNNNGG 2 cut(s) 541, 542
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 1 cut(s) 162
BseXI GCAGC 3 cut(s) 154, 202, 490
BsgI GTGCAG 1 cut(s) 249
BshFI GGCC 1 cut(s) 290
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 2 cut(s) 541, 542
BsmAI GTCTC 3 cut(s) 183, 321, 585
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 1 cut(s) 290
Bso31I GGTCTC 1 cut(s) 183
Bsp19I CCATGG 1 cut(s) 374
BspACI CCGC 1 cut(s) 287
BspANI GGCC 1 cut(s) 290
BspCNI CTCAG 1 cut(s) 161
BspLI GGNNCC 2 cut(s) 533, 534
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 411
BssECI CCNNGG 1 cut(s) 374
BssT1I CCWWGG 1 cut(s) 374
BstDEI CTNAG 2 cut(s) 148, 269
BstDSI CCRYGG 1 cut(s) 374
BstMAI GTCTC 3 cut(s) 183, 321, 585
BstV1I GCAGC 3 cut(s) 154, 202, 490
BstV2I GAAGAC 1 cut(s) 405
BsuRI GGCC 1 cut(s) 290
BtgI CCRYGG 1 cut(s) 374
Cfr13I GGNCC 1 cut(s) 532
Csp6I GTAC 1 cut(s) 60
CviAII CATG 1 cut(s) 375
CviJI RGCY 9 cut(s) 39, 147, 167, 260, 290, 337, 382, 473, 554
CviKI_1 RGCY 9 cut(s) 39, 147, 167, 260, 290, 337, 382, 473, 554
CviQI GTAC 1 cut(s) 60
DdeI CTNAG 2 cut(s) 148, 269
EaeI YGGCCR 1 cut(s) 288
Eco130I CCWWGG 1 cut(s) 374
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 532
EcoO109I RGGNCCY 1 cut(s) 532
EcoT14I CCWWGG 1 cut(s) 374
ErhI CCWWGG 1 cut(s) 374
FaeI CATG 1 cut(s) 378
FaiI YATR 8 cut(s) 14, 108, 195, 376, 447, 498, 515, 549
FaqI GGGAC 1 cut(s) 518
FatI CATG 1 cut(s) 374
Fnu4HI GCNGC 4 cut(s) 168, 216, 288, 504
Fsp4HI GCNGC 4 cut(s) 168, 216, 288, 504
FspBI CTAG 4 cut(s) 57, 63, 171, 206
GluI GCNGC 4 cut(s) 168, 216, 288, 504
HaeIII GGCC 1 cut(s) 290
Hin1II CATG 1 cut(s) 378
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 2 cut(s) 161, 329
HpaI GTTAAC 1 cut(s) 52
Hpy166II GTNNAC 2 cut(s) 52, 574
Hpy8I GTNNAC 2 cut(s) 52, 574
HpyAV CCTTC 2 cut(s) 150, 317
HpyCH4IV ACGT 1 cut(s) 567
HpyCH4V TGCA 5 cut(s) 218, 266, 350, 416, 485
HpyF3I CTNAG 2 cut(s) 148, 269
HpySE526I ACGT 1 cut(s) 567
Hsp92II CATG 1 cut(s) 378
KflI GGGWCCC 1 cut(s) 532
KspAI GTTAAC 1 cut(s) 52
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 2 cut(s) 323, 342
Lsp1109I GCAGC 3 cut(s) 154, 202, 490
LweI GCATC 2 cut(s) 337, 466
MaeI CTAG 4 cut(s) 57, 63, 171, 206
MaeII ACGT 1 cut(s) 567
MaeIII GTNAC 1 cut(s) 352
MboII GAAGA 3 cut(s) 231, 410, 590
MfeI CAATTG 1 cut(s) 6
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 3 cut(s) 123, 372, 535
MroXI GAANNNNTTC 1 cut(s) 160
MseI TTAA 8 cut(s) 51, 99, 129, 281, 441, 491, 557, 604
MspA1I CMGCKG 1 cut(s) 167
MunI CAATTG 1 cut(s) 6
NcoI CCATGG 1 cut(s) 374
NlaIII CATG 1 cut(s) 378
NlaIV GGNNCC 2 cut(s) 533, 534
PdmI GAANNNNTTC 1 cut(s) 160
PfeI GAWTC 1 cut(s) 161
PkrI GCNGC 4 cut(s) 169, 217, 289, 505
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
PpuMI RGGWCCY 1 cut(s) 532
Psp5II RGGWCCY 1 cut(s) 532
PspN4I GGNNCC 2 cut(s) 533, 534
PspPI GGNCC 1 cut(s) 532
PspPPI RGGWCCY 1 cut(s) 532
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
SaqAI TTAA 8 cut(s) 51, 99, 129, 281, 441, 491, 557, 604
SatI GCNGC 4 cut(s) 168, 216, 288, 504
Sau96I GGNCC 1 cut(s) 532
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 1 cut(s) 323
SetI ASST 9 cut(s) 41, 87, 169, 262, 304, 339, 440, 475, 570
SfaNI GCATC 2 cut(s) 337, 466
SinI GGWCC 1 cut(s) 532
SpeI ACTAGT 3 cut(s) 56, 62, 205
SsiI CCGC 1 cut(s) 287
SspMI CTAG 4 cut(s) 57, 63, 171, 206
StyI CCWWGG 1 cut(s) 374
TaiI ACGT 1 cut(s) 570
TatI WGTACW 1 cut(s) 59
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 1 cut(s) 161
Tru1I TTAA 8 cut(s) 51, 99, 129, 281, 441, 491, 557, 604
Tru9I TTAA 8 cut(s) 51, 99, 129, 281, 441, 491, 557, 604
TseI GCWGC 3 cut(s) 167, 215, 503
TspDTI ATGAA 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 532
XapI RAATTY 3 cut(s) 136, 418, 487
XmnI GAANNNNTTC 1 cut(s) 160
XspI CTAG 4 cut(s) 57, 63, 171, 206
ZrmI AGTACT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.