Rorug07G0205400

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000710
Physical Location & Seq
Reverse (-)
18059081 .. 18062599
3519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0205400.1

Sequence Viewer

Length: 2370 bp
ATGGATTCAAAAGCCAGGAGAAGAGCTTCTATCGAGAATGGGGAAGCCGGGGAGGACTTGGTTCTCGCTACGTTGATTGGGAATGGAGATGACCTGGGTCCTATTGTCAGGCATGCATTTGAGATGGGGAGGCCGGAATCGCTCCTTCAGCAGCTCAAGCATGTGGTGAGAAAGAAGGAAGTCGAGATTGAGGATCTGTGTAAGACTCATTACGAGGAGTTCATTCTTGCAGTTGACGAGCTTCGTGGGGTGTTGGTGGATGCTGAAGAGCTGAAAGGCGAGCTCTCTAGTGATAATTTTAAGTTGCAAGAGGTTGGGAGTAGTTTGTTGATTAAACTTGAGGAGCTTCTCGAGTCTTATTCAATCAAAAAGAATGTGGCTGAAGCTATCAAAATGTCCAAGAATTGTGTACAGGTGCTGGAGCTCTGCGTTAAGTTTAACAAGCACATATCCGAAGGCCAGTTTTACCCAGCTTTGAAAACCTTAGATCTGATTGAGAAAAGTTACTTGAAAAATGTTCCTGTCAGGACACTAAGAATGGTTATAGAGAAGAGGATTCCATTGATTAAATTGCACATCGAGAAGAAAGTAACTAGTCAATTCAATGAATGGCTGGTTCAGATAAGAAGTTCTGCAAAGGATATTGGACAAACAGCAATAGGCCAAGCTGCTTCTGCGCGCCAGAGAGATGAGGAAATGCTGGATCGGCAAAGGAAAGCTGAGGAACAAAATCTTCCGGGGTTGGGAGAGTTTGCGTATACTTTAGATGTTGAAGAGATTGAAGAAGAATCAGTTCTAAAGGTTGACCTAACTCCTCTATACAGGGCTTATCACATCCAGAGTTGCCTAGGAATCCAAGAGCAGTTTTGGGAATATTACTATAGAAATCGATTGTTGCAGCTTAATTCAGACTTACAGATCTCTTCAACACAGCCCTTTGTTGAATCCTATCAAACATTTTTGGCTCAAATTGCAGGATACTTCATAGTGGAGGATCGTGTCCTGAGGACTGCTGGTGGCCTACGTTTAGGTGAACAAGTTGAAACAATGTGGGAAACAGCTGTAGCGAAAATGAAATCTCTGTTAGAGGTGCAGTTCTCCCAGATGAATTCTGCAACCCATCTTCTCTTGGTGAAGGATTATGTGACTCTTCTCGGCTGTACTCTTAGACAATATGGGTATGAAGTGGGACCACTTCTTGAGACACTTAACAAGAGCCGAGACAAATACCACGAGCTTCTTTCAGAAGAGTGTCGTAAACAAATTGTTAATGTTATTGCCAATGATACCTATGAGCAGATGGTGTTGAAAAAGGAAAGTGACTATGAAAATAATGTCTTGTCCTTCAATCTCCAGACAACAGATATCACACCGGCTTTTCCATTCATTGCACCATTCTCCTCCACAGTACCTGATGCCTGCCGAATTGTCAGATCATTCATCAAAGGGTCTGTTGATTACTTGACCTATGGGACAAATACTAATGTTTACGATGTTGTGAAGAAATATTTGGATAAATTCTTGATAGATGTGTTAAATGAGGTGATACTTAATACTATTCAAGGTGGCAGCATTGGTGTCTCTCAGGCCATGCAGATTGCTGCGAACATATCTGTACTTGAAGGAGCTTGTGACTTTTTCCTTCGACATGCAGCCCAACTATGTGGGATTCCAAGTCGTTCAGTTGAGAGGCCTCAAGCTGGTTTAACTGCTAAGGTAGTTCTAAAGACTTCTAGGGATGAAGCTTATCTTGCTTTGCTGAATTTGGTGAATGCGAAGTTGGATCAGTTCATGCAACTTACAGAAAATATCAATTGGACTTCAGAGGAACCAACACAGGGTGAAAATGAGTACATGAATGAAGTTGTTATTTACCTCGATACTCTTATGTCCACTGCGCAACAAATCTTACCTTTGGATGCTCTGTACAAGGTTGGGAGTGGGGCTCTTGATCACATTTCCAACTCTATAGTATCAGCTTTTCTTAGCGGTAGCATAAAGAGGTTCAATGCTAATGCAGTTATGAGTATCAACAATGATTTGAAGATGGTGGAGTCTTTTGCAGATGAGAGGTTTCACAGTACTGGCTTGAGCGAAATTTACAAAGACGGAAGTTTTAGAGGTTGCTTGATAGAGGCAAGACAACTGATAAACCTTTTGTCAAGCAGTCAGCCAGAGAATTTCATGAATCCCGTGATAAGGGAGAAAAACTACAATACTTTGGATTATAAAAAGGTGGCCAGTATCTGCGAGAAATTCAAGGATTCTGCTGATGGGATTTTTGGAAGCCTTTCGAACAAGAATAACAAGCAAAATGCTCGTAAGAAATCAATGGACATGCTTAAGAAAAGATTGAAGGACTTCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

789

Amino Acids

89.39

Weight (kDa)

5.46

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXOC6_Sec15_N PF20651 51 - 220 9.3e-54 Exocyst complex component EXOC6/Sec15, N-terminal
Sec15_C PF04091 398 - 752 2e-101 Exocyst complex subunit Sec15 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2229
Acc16I TGCGCA 1 cut(s) 1899
AccI GTMKAC 1 cut(s) 758
AccII CGCG 1 cut(s) 679
AciI CCGC 1 cut(s) 1989
AclWI GGATC 4 cut(s) 201, 711, 1002, 1791
AcoI YGGCCR 1 cut(s) 2238
AcsI RAATTY 6 cut(s) 1108, 1517, 1762, 2097, 2179, 2255
AcuI CTGAAG 4 cut(s) 131, 285, 402, 1806
AdeI CACNNNGTG 1 cut(s) 1841
AfaI GTAC 7 cut(s) 411, 1162, 1410, 1617, 1853, 1928, 2083
AfiI CCNNNNNNNGG 4 cut(s) 743, 1700, 1838, 2199
AflII CTTAAG 1 cut(s) 2342
AhlI ACTAGT 1 cut(s) 593
AjnI CCWGG 2 cut(s) 14, 93
AjuI GAANNNNNNNTTGG 4 cut(s) 1493, 1525, 1763, 1795
Alw21I GWGCWC 2 cut(s) 285, 426
Alw26I GTCTC 3 cut(s) 1196, 1215, 1585
AlwI GGATC 4 cut(s) 201, 711, 1002, 1791
AlwNI CAGNNNCTG 3 cut(s) 418, 1412, 2247
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 7 cut(s) 131, 457, 661, 1018, 1587, 1691, 2238
ApeKI GCWGC 6 cut(s) 151, 668, 898, 1569, 1601, 1652
ApoI RAATTY 6 cut(s) 1108, 1517, 1762, 2097, 2179, 2255
Asp700I GAANNNNTTC 4 cut(s) 25, 792, 2290, 2360
AspA2I CCTAGG 1 cut(s) 847
AspLEI GCGC 3 cut(s) 679, 681, 1900
AspS9I GGNCC 2 cut(s) 98, 1190
AsuC2I CCSGG 2 cut(s) 49, 738
AsuHPI GGTGA 6 cut(s) 178, 1043, 1144, 1555, 1780, 1853
AsuII TTCGAA 1 cut(s) 2294
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 2 cut(s) 98, 1190
AvrII CCTAGG 1 cut(s) 847
AxyI CCTNAGG 1 cut(s) 1004
BalI TGGCCA 1 cut(s) 2240
BanII GRGCYC 3 cut(s) 285, 426, 1948
BauI CACGAG 1 cut(s) 1232
Bbv12I GWGCWC 2 cut(s) 285, 426
BbvCI CCTCAGC 1 cut(s) 720
BbvI GCAGC 6 cut(s) 163, 655, 910, 1581, 1588, 1664
BccI CCATC 5 cut(s) 118, 1128, 1294, 2041, 2267
BcgI CGANNNNNNTGC 2 cut(s) 2300, 2334
BciT130I CCWGG 2 cut(s) 16, 95
BciVI GTATCC 1 cut(s) 971
BclI TGATCA 1 cut(s) 1951
BcnI CCSGG 2 cut(s) 49, 738
BcoDI GTCTC 3 cut(s) 1196, 1215, 1585
BcuI ACTAGT 1 cut(s) 593
BfaI CTAG 4 cut(s) 288, 594, 848, 1734
BfmI CTRYAG 3 cut(s) 880, 1062, 1968
BfrI CTTAAG 1 cut(s) 2342
BfuI GTATCC 1 cut(s) 971
BglII AGATCT 2 cut(s) 487, 918
BisI GCNGC 6 cut(s) 152, 669, 899, 1570, 1602, 1653
BlnI CCTAGG 1 cut(s) 847
BlsI GCNGC 6 cut(s) 153, 670, 900, 1571, 1603, 1654
BmcAI AGTACT 1 cut(s) 2083
Bme1390I CCNGG 4 cut(s) 16, 49, 95, 738
Bme18I GGWCC 2 cut(s) 98, 1190
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 2 cut(s) 98, 1190
BmiI GGNNCC 3 cut(s) 99, 1191, 1830
BmrFI CCNGG 4 cut(s) 16, 49, 95, 738
BmsI GCATC 3 cut(s) 250, 1405, 1909
BoxI GACNNNNGTC 1 cut(s) 96
BplI GAGNNNNNCTC 2 cut(s) 1930, 1962
BpmI CTGGAG 2 cut(s) 440, 1337
Bpu10I CCTNAGC 2 cut(s) 720, 1713
Bpu14I TTCGAA 1 cut(s) 2294
BpuEI CTTGAG 5 cut(s) 140, 359, 1220, 1680, 2110
BpuMI CCSGG 2 cut(s) 49, 738
Bsa29I ATCGAT 1 cut(s) 889
BsaJI CCNNGG 4 cut(s) 48, 94, 737, 847
Bsc4I CCNNNNNNNGG 4 cut(s) 743, 1700, 1838, 2199
Bse118I RCCGGY 1 cut(s) 1372
Bse1I ACTGG 3 cut(s) 460, 2089, 2241
Bse21I CCTNAGG 1 cut(s) 1004
Bse3DI GCAATG 1 cut(s) 1386
BseBI CCWGG 2 cut(s) 16, 95
BseCI ATCGAT 1 cut(s) 889
BseDI CCNNGG 4 cut(s) 48, 94, 737, 847
BseGI GGATG 4 cut(s) 265, 834, 1744, 1924
BseLI CCNNNNNNNGG 4 cut(s) 743, 1700, 1838, 2199
BseMI GCAATG 1 cut(s) 1386
BseMII CTCAG 3 cut(s) 711, 995, 1598
BseNI ACTGG 3 cut(s) 460, 2089, 2241
BsePI GCGCGC 1 cut(s) 677
BseRI GAGGAG 4 cut(s) 230, 356, 804, 1390
BseXI GCAGC 6 cut(s) 163, 655, 910, 1581, 1588, 1664
BseYI CCCAGC 1 cut(s) 469
BsgI GTGCAG 1 cut(s) 1112
Bsh1236I CGCG 1 cut(s) 679
BshFI GGCC 7 cut(s) 133, 459, 663, 1020, 1589, 1693, 2240
BshVI ATCGAT 1 cut(s) 889
BsiHKAI GWGCWC 2 cut(s) 285, 426
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 4 cut(s) 48, 134, 737, 1373
BslFI GGGAC 2 cut(s) 1203, 1486
BslI CCNNNNNNNGG 4 cut(s) 743, 1700, 1838, 2199
BsmAI GTCTC 3 cut(s) 1196, 1215, 1585
BsmFI GGGAC 2 cut(s) 1203, 1486
BsmI GAATGC 1 cut(s) 1777
BsnI GGCC 7 cut(s) 133, 459, 663, 1020, 1589, 1693, 2240
BsoBI CYCGRG 1 cut(s) 350
Bsp119I TTCGAA 1 cut(s) 2294
Bsp1286I GDGCHC 3 cut(s) 285, 426, 1948
Bsp1407I TGTACA 2 cut(s) 409, 1926
Bsp143I GATC 8 cut(s) 193, 487, 703, 918, 994, 1433, 1783, 1951
BspACI CCGC 1 cut(s) 1989
BspANI GGCC 7 cut(s) 133, 459, 663, 1020, 1589, 1693, 2240
BspCNI CTCAG 3 cut(s) 712, 996, 1597
BspDI ATCGAT 1 cut(s) 889
BspFNI CGCG 1 cut(s) 679
BspHI TCATGA 1 cut(s) 2184
BspLI GGNNCC 3 cut(s) 99, 1191, 1830
BspPI GGATC 4 cut(s) 201, 711, 1002, 1791
BspQI GCTCTTC 2 cut(s) 16, 261
BspT104I TTCGAA 1 cut(s) 2294
BspTI CTTAAG 1 cut(s) 2342
BsrDI GCAATG 1 cut(s) 1386
BsrFI RCCGGY 1 cut(s) 1372
BsrGI TGTACA 2 cut(s) 409, 1926
BsrI ACTGG 3 cut(s) 460, 2089, 2241
BssAI RCCGGY 1 cut(s) 1372
BssECI CCNNGG 4 cut(s) 48, 94, 737, 847
BssHII GCGCGC 1 cut(s) 677
BssMI GATC 8 cut(s) 193, 487, 703, 918, 994, 1433, 1783, 1951
BssNAI GTATAC 1 cut(s) 759
BssSI CACGAG 1 cut(s) 1232
BssT1I CCWWGG 1 cut(s) 847
Bst1107I GTATAC 1 cut(s) 759
Bst2BI CACGAG 1 cut(s) 1232
Bst2UI CCWGG 2 cut(s) 16, 95
Bst4CI ACNGT 2 cut(s) 1408, 2081
Bst6I CTCTTC 7 cut(s) 16, 261, 545, 768, 928, 1155, 1242
BstAFI CTTAAG 1 cut(s) 2342
BstAUI TGTACA 2 cut(s) 409, 1926
BstBI TTCGAA 1 cut(s) 2294
BstC8I GCNNGC 4 cut(s) 114, 281, 679, 1420
BstDEI CTNAG 8 cut(s) 484, 533, 720, 1004, 1166, 1584, 1713, 1985
BstF5I GGATG 4 cut(s) 265, 834, 1744, 1924
BstFNI CGCG 1 cut(s) 679
BstHHI GCGC 3 cut(s) 679, 681, 1900
BstKTI GATC 8 cut(s) 196, 490, 706, 921, 997, 1436, 1786, 1954
BstMAI GTCTC 3 cut(s) 1196, 1215, 1585
BstMBI GATC 8 cut(s) 193, 487, 703, 918, 994, 1433, 1783, 1951
BstMWI GCNNNNNNNGC 7 cut(s) 139, 148, 157, 674, 706, 971, 1751
BstNI CCWGG 2 cut(s) 16, 95
BstNSI RCATGY 4 cut(s) 116, 164, 1652, 2341
BstPAI GACNNNNGTC 1 cut(s) 96
BstSCI CCNGG 4 cut(s) 14, 47, 93, 736
BstSFI CTRYAG 3 cut(s) 880, 1062, 1968
BstUI CGCG 1 cut(s) 679
BstV1I GCAGC 6 cut(s) 163, 655, 910, 1581, 1588, 1664
BstX2I RGATCY 3 cut(s) 193, 487, 918
BstXI CCANNNNNNTGG 1 cut(s) 1664
BstYI RGATCY 3 cut(s) 193, 487, 918
BstZ17I GTATAC 1 cut(s) 759
Bsu15I ATCGAT 1 cut(s) 889
Bsu36I CCTNAGG 1 cut(s) 1004
BsuI GTATCC 1 cut(s) 971
BsuRI GGCC 7 cut(s) 133, 459, 663, 1020, 1589, 1693, 2240
BsuTUI ATCGAT 1 cut(s) 889
BtsCI GGATG 4 cut(s) 265, 834, 1744, 1924
BtsI GCAGTG 1 cut(s) 1893
BtsIMutI CAGTG 1 cut(s) 1893
Cac8I GCNNGC 4 cut(s) 114, 281, 679, 1420
CaiI CAGNNNCTG 3 cut(s) 418, 1412, 2247
CciI TCATGA 1 cut(s) 2184
CfoI GCGC 3 cut(s) 679, 681, 1900
Cfr10I RCCGGY 1 cut(s) 1372
Cfr13I GGNCC 2 cut(s) 98, 1190
ClaI ATCGAT 1 cut(s) 889
Csp6I GTAC 7 cut(s) 410, 1161, 1409, 1616, 1852, 1927, 2082
CviAII CATG 8 cut(s) 113, 161, 1591, 1649, 1792, 1855, 2185, 2338
CviQI GTAC 7 cut(s) 410, 1161, 1409, 1616, 1852, 1927, 2082
DdeI CTNAG 8 cut(s) 484, 533, 720, 1004, 1166, 1584, 1713, 1985
DpnI GATC 8 cut(s) 195, 489, 705, 920, 996, 1435, 1785, 1953
DpnII GATC 8 cut(s) 193, 487, 703, 918, 994, 1433, 1783, 1951
DraIII CACNNNGTG 1 cut(s) 1841
EaeI YGGCCR 1 cut(s) 2238
Eam1104I CTCTTC 7 cut(s) 16, 261, 545, 768, 928, 1155, 1242
EarI CTCTTC 7 cut(s) 16, 261, 545, 768, 928, 1155, 1242
Ecl136II GAGCTC 2 cut(s) 283, 424
Eco130I CCWWGG 1 cut(s) 847
Eco147I AGGCCT 1 cut(s) 1693
Eco24I GRGCYC 3 cut(s) 285, 426, 1948
Eco32I GATATC 1 cut(s) 1366
Eco47I GGWCC 2 cut(s) 98, 1190
Eco53kI GAGCTC 2 cut(s) 283, 424
Eco57I CTGAAG 4 cut(s) 131, 285, 402, 1806
Eco81I CCTNAGG 1 cut(s) 1004
Eco88I CYCGRG 1 cut(s) 350
EcoICRI GAGCTC 2 cut(s) 283, 424
EcoO109I RGGNCCY 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 1108
EcoRII CCWGG 2 cut(s) 14, 93
EcoRV GATATC 1 cut(s) 1366
EcoT14I CCWWGG 1 cut(s) 847
EcoT22I ATGCAT 1 cut(s) 118
EcoT38I GRGCYC 3 cut(s) 285, 426, 1948
ErhI CCWWGG 1 cut(s) 847
FaeI CATG 8 cut(s) 116, 164, 1594, 1652, 1795, 1858, 2188, 2341
FalI AAGNNNNNCTT 2 cut(s) 1734, 1766
FaqI GGGAC 2 cut(s) 1203, 1486
FatI CATG 8 cut(s) 112, 160, 1590, 1648, 1791, 1854, 2184, 2337
FbaI TGATCA 1 cut(s) 1951
FblI GTMKAC 1 cut(s) 758
Fnu4HI GCNGC 6 cut(s) 152, 669, 899, 1570, 1602, 1653
FokI GGATG 4 cut(s) 272, 821, 1751, 1931
FriOI GRGCYC 3 cut(s) 285, 426, 1948
Fsp4HI GCNGC 6 cut(s) 152, 669, 899, 1570, 1602, 1653
FspBI CTAG 4 cut(s) 288, 594, 848, 1734
FspI TGCGCA 1 cut(s) 1899
GlaI GCGC 3 cut(s) 678, 680, 1899
GluI GCNGC 6 cut(s) 152, 669, 899, 1570, 1602, 1653
GsaI CCCAGC 1 cut(s) 473
GsuI CTGGAG 2 cut(s) 440, 1337
HaeIII GGCC 7 cut(s) 133, 459, 663, 1020, 1589, 1693, 2240
HapII CCGG 4 cut(s) 48, 134, 737, 1373
HhaI GCGC 3 cut(s) 679, 681, 1900
Hin1II CATG 8 cut(s) 116, 164, 1594, 1652, 1795, 1858, 2188, 2341
Hin6I GCGC 3 cut(s) 677, 679, 1898
HinP1I GCGC 3 cut(s) 677, 679, 1898
HincII GTYRAC 2 cut(s) 235, 805
HindII GTYRAC 2 cut(s) 235, 805
HindIII AAGCTT 1 cut(s) 1743
HpaII CCGG 4 cut(s) 48, 134, 737, 1373
HphI GGTGA 6 cut(s) 178, 1043, 1144, 1555, 1780, 1853
Hpy166II GTNNAC 8 cut(s) 235, 410, 759, 805, 1034, 1259, 1489, 1893
Hpy188I TCNGA 7 cut(s) 454, 492, 621, 910, 1246, 1433, 1825
Hpy8I GTNNAC 8 cut(s) 235, 410, 759, 805, 1034, 1259, 1489, 1893
HpyAV CCTTC 8 cut(s) 155, 169, 449, 1129, 1354, 1616, 1652, 2350
HpyCH4III ACNGT 2 cut(s) 1408, 2081
HpyCH4IV ACGT 2 cut(s) 71, 1024
HpyF10VI GCNNNNNNNGC 7 cut(s) 139, 148, 157, 674, 706, 971, 1751
HpyF3I CTNAG 8 cut(s) 484, 533, 720, 1004, 1166, 1584, 1713, 1985
HpySE526I ACGT 2 cut(s) 71, 1024
Hsp92II CATG 8 cut(s) 116, 164, 1594, 1652, 1795, 1858, 2188, 2341
HspAI GCGC 3 cut(s) 677, 679, 1898
Ksp22I TGATCA 1 cut(s) 1951
Kzo9I GATC 8 cut(s) 193, 487, 703, 918, 994, 1433, 1783, 1951
LguI GCTCTTC 2 cut(s) 16, 261
LmnI GCTCC 4 cut(s) 147, 343, 421, 1625
Lsp1109I GCAGC 6 cut(s) 163, 655, 910, 1581, 1588, 1664
LweI GCATC 3 cut(s) 250, 1405, 1909
MaeI CTAG 4 cut(s) 288, 594, 848, 1734
MaeII ACGT 2 cut(s) 71, 1024
MaeIII GTNAC 5 cut(s) 503, 589, 1144, 1319, 1631
MalI GATC 8 cut(s) 195, 489, 705, 920, 996, 1435, 1785, 1953
MboI GATC 8 cut(s) 193, 487, 703, 918, 994, 1433, 1783, 1951
MfeI CAATTG 1 cut(s) 1813
MflI RGATCY 3 cut(s) 193, 487, 918
MhlI GDGCHC 3 cut(s) 285, 426, 1948
MlsI TGGCCA 1 cut(s) 2240
MluNI TGGCCA 1 cut(s) 2240
MlyI GAGTC 4 cut(s) 199, 362, 1141, 2063
MmeI TCCRAC 2 cut(s) 1761, 1986
Mox20I TGGCCA 1 cut(s) 2240
Mph1103I ATGCAT 1 cut(s) 118
MroXI GAANNNNTTC 4 cut(s) 25, 792, 2290, 2360
MscI TGGCCA 1 cut(s) 2240
Msp20I TGGCCA 1 cut(s) 2240
MspA1I CMGCKG 1 cut(s) 1061
MspCI CTTAAG 1 cut(s) 2342
MspI CCGG 4 cut(s) 48, 134, 737, 1373
MspR9I CCNGG 4 cut(s) 16, 49, 95, 738
MunI CAATTG 1 cut(s) 1813
Mva1269I GAATGC 1 cut(s) 1777
MvaI CCWGG 2 cut(s) 16, 95
MvnI CGCG 1 cut(s) 679
MwoI GCNNNNNNNGC 7 cut(s) 139, 148, 157, 674, 706, 971, 1751
NciI CCSGG 2 cut(s) 49, 738
NdeII GATC 8 cut(s) 193, 487, 703, 918, 994, 1433, 1783, 1951
NlaIII CATG 8 cut(s) 116, 164, 1594, 1652, 1795, 1858, 2188, 2341
NlaIV GGNNCC 3 cut(s) 99, 1191, 1830
NmeAIII GCCGAG 2 cut(s) 1134, 1244
NmuCI GTSAC 3 cut(s) 1144, 1319, 1631
NsbI TGCGCA 1 cut(s) 1899
NsiI ATGCAT 1 cut(s) 118
NspI RCATGY 4 cut(s) 116, 164, 1652, 2341
NspV TTCGAA 1 cut(s) 2294
PaeI GCATGC 1 cut(s) 116
PaeR7I CTCGAG 1 cut(s) 350
PagI TCATGA 1 cut(s) 2184
PauI GCGCGC 1 cut(s) 677
PceI AGGCCT 1 cut(s) 1693
PciSI GCTCTTC 2 cut(s) 16, 261
PctI GAATGC 1 cut(s) 1777
PdmI GAANNNNTTC 4 cut(s) 25, 792, 2290, 2360
PfeI GAWTC 9 cut(s) 5, 137, 556, 788, 852, 944, 1669, 2188, 2264
PkrI GCNGC 6 cut(s) 153, 670, 900, 1571, 1603, 1654
PleI GAGTC 4 cut(s) 199, 361, 1141, 2062
PpsI GAGTC 4 cut(s) 199, 361, 1141, 2062
PpuMI RGGWCCY 1 cut(s) 98
PshAI GACNNNNGTC 1 cut(s) 96
PsiI TTATAA 1 cut(s) 2229
Psp124BI GAGCTC 2 cut(s) 285, 426
Psp5II RGGWCCY 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 14, 93
PspFI CCCAGC 1 cut(s) 469
PspGI CCWGG 2 cut(s) 14, 93
PspN4I GGNNCC 3 cut(s) 99, 1191, 1830
PspPI GGNCC 2 cut(s) 98, 1190
PspPPI RGGWCCY 1 cut(s) 98
PstNI CAGNNNCTG 3 cut(s) 418, 1412, 2247
PsuI RGATCY 3 cut(s) 193, 487, 918
PteI GCGCGC 1 cut(s) 677
PvuII CAGCTG 1 cut(s) 1061
RsaI GTAC 7 cut(s) 411, 1162, 1410, 1617, 1853, 1928, 2083
RsaNI GTAC 7 cut(s) 410, 1161, 1409, 1616, 1852, 1927, 2082
SacI GAGCTC 2 cut(s) 285, 426
SapI GCTCTTC 2 cut(s) 16, 261
SatI GCNGC 6 cut(s) 152, 669, 899, 1570, 1602, 1653
Sau3AI GATC 8 cut(s) 193, 487, 703, 918, 994, 1433, 1783, 1951
Sau96I GGNCC 2 cut(s) 98, 1190
ScaI AGTACT 1 cut(s) 2083
SchI GAGTC 4 cut(s) 199, 362, 1141, 2063
ScrFI CCNGG 4 cut(s) 16, 49, 95, 738
SduI GDGCHC 3 cut(s) 285, 426, 1948
SfaNI GCATC 3 cut(s) 250, 1405, 1909
SfcI CTRYAG 3 cut(s) 880, 1062, 1968
Sfr274I CTCGAG 1 cut(s) 350
SfuI TTCGAA 1 cut(s) 2294
SinI GGWCC 2 cut(s) 98, 1190
SlaI CTCGAG 1 cut(s) 350
SmlI CTYRAG 7 cut(s) 155, 338, 350, 1199, 1695, 2089, 2342
SmoI CTYRAG 7 cut(s) 155, 338, 350, 1199, 1695, 2089, 2342
SpeI ACTAGT 1 cut(s) 593
SphI GCATGC 1 cut(s) 116
SseBI AGGCCT 1 cut(s) 1693
SsiI CCGC 1 cut(s) 1989
SspI AATATT 2 cut(s) 875, 1508
SspMI CTAG 4 cut(s) 288, 594, 848, 1734
SstI GAGCTC 2 cut(s) 285, 426
StuI AGGCCT 1 cut(s) 1693
StyD4I CCNGG 4 cut(s) 14, 47, 93, 736
StyI CCWWGG 1 cut(s) 847
TaaI ACNGT 2 cut(s) 1408, 2081
TaiI ACGT 2 cut(s) 74, 1027
TaqI TCGA 8 cut(s) 33, 183, 351, 579, 889, 1645, 1878, 2294
TatI WGTACW 6 cut(s) 409, 1160, 1615, 1851, 1926, 2081
TfiI GAWTC 9 cut(s) 5, 137, 556, 788, 852, 944, 1669, 2188, 2264
TscAI CASTG 1 cut(s) 1900
TseFI GTSAC 3 cut(s) 1144, 1319, 1631
TseI GCWGC 6 cut(s) 151, 668, 898, 1569, 1601, 1652
Tsp45I GTSAC 3 cut(s) 1144, 1319, 1631
TspGWI ACGGA 1 cut(s) 2124
TspRI CASTG 1 cut(s) 1900
Vha464I CTTAAG 1 cut(s) 2342
VpaK11BI GGWCC 2 cut(s) 98, 1190
XapI RAATTY 6 cut(s) 1108, 1517, 1762, 2097, 2179, 2255
XceI RCATGY 4 cut(s) 116, 164, 1652, 2341
XhoI CTCGAG 1 cut(s) 350
XmaJI CCTAGG 1 cut(s) 847
XmiI GTMKAC 1 cut(s) 758
XmnI GAANNNNTTC 4 cut(s) 25, 792, 2290, 2360
XspI CTAG 4 cut(s) 288, 594, 848, 1734
ZrmI AGTACT 1 cut(s) 2083
Zsp2I ATGCAT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.