Rorug05G0353400

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000710
Physical Location & Seq
Reverse (-)
44114038 .. 44114952
915 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0353400.1

Sequence Viewer

Length: 915 bp
ATGCTTGCAAATGTACATGTATGTACATTTGCTAAAGTGTTCAAGCACCGTCCTATCAACACTGGCTCCCCTACTCAATCCACAGCACCCAAGAAAGTTCAAATCCTTGAAGAGGAAGAATATGAACAACCACCAAGAGTGCCAATCACCCTTCGAGAATTCTTACCTAAAGAACTTCTTGAAGACGGACAACAAGAGGATGGTGAAGAGGTGTTTGGTTGTTGTATGGTCACCATCGAGGGTCCTAAGGGATCAGACATCGGGGCGGAAGACAAAAGTTCAGATAAGTCCGAACCTGAGGTTAATAAGATCACTTTACGCTCTGGTTTGAGTATTCCAGGAGTTGACAAGCAGAAAGAAGTAAATGTCCCATCTGATATCCTGAAAAAGTCAAAGGTGAAAATCGTTCCAGAGAACACGGGGGACAGGACTGCAATAGCTTCGACGTCGAAGAACTCTAAACAGGAACAGAACGAGAGCCAAGGAAGGGAGTCATCACACAAATGCAAATACAATGTGTTGGCTCACCTGAAGCGTGTCTCGGCTATGTTGAGTGTCTATGACGCACTACAGACATCAAGAGAATTGAGGGAAACACTCATTGAAGCTTTAATGAGCCCTGACATGTATAAGGAAGAAATGTCAGAGGTCGCCATAACCACGCCAAGAGTTTGTACCAACTACATGGAGTGCATTACCTTTGGTGAGGAAGACCTTCTGCTAGGATCCGAGTACCACAACAGACCTCTCTATGTCACAGGACTTGTGGGCAATACGACTCTCAATCTGATTCTCCTAGATGATGGCTCAGATGTAAACCTCATGCCACTCAAGACGCTCCACATGATGGGAATGAGTGCAAAACAAGTATCCCCATCCATGTTGACAATCCAAGGCTATAATCAATCTGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

304

Amino Acids

33.71

Weight (kDa)

5.25

Isoelectric Point (pI)

61.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 449
AccB7I CCANNNNNTGG 1 cut(s) 847
AciI CCGC 1 cut(s) 266
AclWI GGATC 3 cut(s) 259, 720, 733
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 551
AcyI GRCGYC 1 cut(s) 446
AfaI GTAC 4 cut(s) 15, 25, 676, 734
AfiI CCNNNNNNNGG 3 cut(s) 112, 487, 847
AflIII ACRYGT 2 cut(s) 16, 624
AgsI TTSAA 5 cut(s) 43, 101, 110, 182, 605
AjnI CCWGG 1 cut(s) 337
AjuI GAANNNNNNNTTGG 2 cut(s) 198, 230
AluBI AGCT 2 cut(s) 440, 608
AluI AGCT 2 cut(s) 440, 608
Alw26I GTCTC 1 cut(s) 544
AlwI GGATC 3 cut(s) 259, 720, 733
ApoI RAATTY 1 cut(s) 158
Asp700I GAANNNNTTC 1 cut(s) 714
AspS9I GGNCC 1 cut(s) 242
AsuHPI GGTGA 6 cut(s) 139, 215, 223, 409, 518, 716
AvaII GGWCC 1 cut(s) 242
AxyI CCTNAGG 2 cut(s) 246, 297
BamHI GGATCC 1 cut(s) 725
BanII GRGCYC 1 cut(s) 620
BbsI GAAGAC 3 cut(s) 189, 276, 717
BccI CCATC 6 cut(s) 194, 242, 379, 797, 841, 883
BcgI CGANNNNNNTGC 2 cut(s) 423, 457
BciT130I CCWGG 1 cut(s) 339
BciVI GTATCC 1 cut(s) 880
BcoDI GTCTC 1 cut(s) 544
BfaI CTAG 2 cut(s) 722, 797
BfmI CTRYAG 1 cut(s) 569
BfuI GTATCC 1 cut(s) 880
Bme1390I CCNGG 1 cut(s) 339
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 242
BmiI GGNNCC 3 cut(s) 67, 243, 727
BmrFI CCNGG 1 cut(s) 339
BpiI GAAGAC 3 cut(s) 189, 276, 717
BpuEI CTTGAG 1 cut(s) 815
BsaHI GRCGYC 1 cut(s) 446
BsaJI CCNNGG 2 cut(s) 481, 892
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 487, 847
Bse1I ACTGG 1 cut(s) 67
Bse21I CCTNAGG 2 cut(s) 246, 297
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 481, 892
BseGI GGATG 2 cut(s) 205, 875
BseLI CCNNNNNNNGG 3 cut(s) 112, 487, 847
BseMII CTCAG 2 cut(s) 288, 822
BseNI ACTGG 1 cut(s) 67
BslFI GGGAC 2 cut(s) 353, 437
BslI CCNNNNNNNGG 3 cut(s) 112, 487, 847
BsmAI GTCTC 1 cut(s) 544
BsmFI GGGAC 2 cut(s) 353, 437
Bsp1286I GDGCHC 1 cut(s) 620
Bsp1407I TGTACA 2 cut(s) 13, 23
Bsp143I GATC 3 cut(s) 251, 309, 725
BspACI CCGC 1 cut(s) 266
BspCNI CTCAG 2 cut(s) 289, 821
BspLI GGNNCC 3 cut(s) 67, 243, 727
BspPI GGATC 3 cut(s) 259, 720, 733
BsrGI TGTACA 2 cut(s) 13, 23
BsrI ACTGG 1 cut(s) 67
BssECI CCNNGG 2 cut(s) 481, 892
BssMI GATC 3 cut(s) 251, 309, 725
BssNI GRCGYC 1 cut(s) 446
BssT1I CCWWGG 2 cut(s) 481, 892
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 1 cut(s) 50
Bst6I CTCTTC 2 cut(s) 105, 201
BstACI GRCGYC 1 cut(s) 446
BstAUI TGTACA 2 cut(s) 13, 23
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 3 cut(s) 246, 297, 808
BstEII GGTNACC 1 cut(s) 229
BstENI CCTNNNNNAGG 1 cut(s) 110
BstF5I GGATG 2 cut(s) 205, 875
BstKTI GATC 3 cut(s) 254, 312, 728
BstMAI GTCTC 1 cut(s) 544
BstMBI GATC 3 cut(s) 251, 309, 725
BstNI CCWGG 1 cut(s) 339
BstNSI RCATGY 2 cut(s) 20, 628
BstPI GGTNACC 1 cut(s) 229
BstSCI CCNGG 1 cut(s) 337
BstSFI CTRYAG 1 cut(s) 569
BstV2I GAAGAC 3 cut(s) 189, 276, 717
BstX2I RGATCY 1 cut(s) 725
BstXI CCANNNNNNTGG 1 cut(s) 685
BstYI RGATCY 1 cut(s) 725
Bsu36I CCTNAGG 2 cut(s) 246, 297
BsuI GTATCC 1 cut(s) 880
BtsCI GGATG 2 cut(s) 205, 875
BtsIMutI CAGTG 1 cut(s) 60
Cac8I GCNNGC 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 242
CseI GACGC 2 cut(s) 572, 844
Csp6I GTAC 4 cut(s) 14, 24, 675, 733
CviAII CATG 6 cut(s) 17, 625, 685, 823, 844, 880
CviJI RGCY 9 cut(s) 66, 440, 480, 524, 545, 608, 618, 807, 897
CviKI_1 RGCY 9 cut(s) 66, 440, 480, 524, 545, 608, 618, 807, 897
CviQI GTAC 4 cut(s) 14, 24, 675, 733
DdeI CTNAG 3 cut(s) 246, 297, 808
DpnI GATC 3 cut(s) 253, 311, 727
DpnII GATC 3 cut(s) 251, 309, 725
Eam1104I CTCTTC 2 cut(s) 105, 201
EarI CTCTTC 2 cut(s) 105, 201
EciI GGCGGA 1 cut(s) 281
Eco130I CCWWGG 2 cut(s) 481, 892
Eco24I GRGCYC 1 cut(s) 620
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 1 cut(s) 242
Eco57I CTGAAG 1 cut(s) 551
Eco81I CCTNAGG 2 cut(s) 246, 297
Eco91I GGTNACC 1 cut(s) 229
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoO109I RGGNCCY 1 cut(s) 242
EcoO65I GGTNACC 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 337
EcoRV GATATC 1 cut(s) 379
EcoT14I CCWWGG 2 cut(s) 481, 892
EcoT38I GRGCYC 1 cut(s) 620
ErhI CCWWGG 2 cut(s) 481, 892
FaeI CATG 6 cut(s) 20, 628, 688, 826, 847, 883
FalI AAGNNNNNCTT 2 cut(s) 162, 194
FaqI GGGAC 2 cut(s) 353, 437
FatI CATG 6 cut(s) 16, 624, 684, 822, 843, 879
FokI GGATG 2 cut(s) 212, 862
FriOI GRGCYC 1 cut(s) 620
FspBI CTAG 2 cut(s) 722, 797
HgaI GACGC 2 cut(s) 572, 844
Hin1I GRCGYC 1 cut(s) 446
Hin1II CATG 6 cut(s) 20, 628, 688, 826, 847, 883
HincII GTYRAC 2 cut(s) 346, 885
HindII GTYRAC 2 cut(s) 346, 885
HindIII AAGCTT 1 cut(s) 606
HinfI GANTC 3 cut(s) 491, 778, 790
HphI GGTGA 6 cut(s) 139, 215, 223, 409, 518, 716
Hpy166II GTNNAC 3 cut(s) 346, 817, 885
Hpy188I TCNGA 8 cut(s) 256, 283, 292, 376, 646, 730, 789, 811
Hpy188III TCNNGA 6 cut(s) 155, 179, 382, 410, 579, 832
Hpy8I GTNNAC 3 cut(s) 346, 817, 885
Hpy99I CGWCG 2 cut(s) 448, 451
HpyAV CCTTC 3 cut(s) 161, 480, 725
HpyCH4III ACNGT 1 cut(s) 50
HpyCH4IV ACGT 1 cut(s) 446
HpyCH4V TGCA 5 cut(s) 8, 434, 507, 693, 860
HpyF3I CTNAG 3 cut(s) 246, 297, 808
HpySE526I ACGT 1 cut(s) 446
Hsp92I GRCGYC 1 cut(s) 446
Hsp92II CATG 6 cut(s) 20, 628, 688, 826, 847, 883
Kzo9I GATC 3 cut(s) 251, 309, 725
LmnI GCTCC 2 cut(s) 71, 843
MaeI CTAG 2 cut(s) 722, 797
MaeII ACGT 1 cut(s) 446
MaeIII GTNAC 2 cut(s) 229, 754
MalI GATC 3 cut(s) 253, 311, 727
MboI GATC 3 cut(s) 251, 309, 725
MboII GAAGA 8 cut(s) 122, 128, 194, 218, 281, 463, 647, 722
MflI RGATCY 1 cut(s) 725
MhlI GDGCHC 1 cut(s) 620
MluCI AATT 2 cut(s) 158, 584
MlyI GAGTC 2 cut(s) 500, 772
MroXI GAANNNNTTC 1 cut(s) 714
MseI TTAA 2 cut(s) 303, 611
MslI CAYNNNNRTG 1 cut(s) 502
MspR9I CCNGG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 339
NdeII GATC 3 cut(s) 251, 309, 725
NlaIII CATG 6 cut(s) 20, 628, 688, 826, 847, 883
NlaIV GGNNCC 3 cut(s) 67, 243, 727
NmeAIII GCCGAG 1 cut(s) 521
NmuCI GTSAC 2 cut(s) 229, 754
NspI RCATGY 2 cut(s) 20, 628
PciI ACATGT 2 cut(s) 16, 624
PdmI GAANNNNTTC 1 cut(s) 714
PfeI GAWTC 1 cut(s) 790
PflMI CCANNNNNTGG 1 cut(s) 847
PfoI TCCNGGA 1 cut(s) 337
PleI GAGTC 2 cut(s) 499, 772
PpsI GAGTC 2 cut(s) 499, 772
PpuMI RGGWCCY 1 cut(s) 242
PscI ACATGT 2 cut(s) 16, 624
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 337
PspEI GGTNACC 1 cut(s) 229
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 3 cut(s) 67, 243, 727
PspPI GGNCC 1 cut(s) 242
PspPPI RGGWCCY 1 cut(s) 242
PsuI RGATCY 1 cut(s) 725
RsaI GTAC 4 cut(s) 15, 25, 676, 734
RsaNI GTAC 4 cut(s) 14, 24, 675, 733
RseI CAYNNNNRTG 1 cut(s) 502
SaqAI TTAA 2 cut(s) 303, 611
Sau3AI GATC 3 cut(s) 251, 309, 725
Sau96I GGNCC 1 cut(s) 242
SchI GAGTC 2 cut(s) 500, 772
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 1 cut(s) 620
SfcI CTRYAG 1 cut(s) 569
SinI GGWCC 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 502
SmlI CTYRAG 1 cut(s) 830
SmoI CTYRAG 1 cut(s) 830
Sse9I AATT 2 cut(s) 158, 584
SsiI CCGC 1 cut(s) 266
SspMI CTAG 2 cut(s) 722, 797
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 2 cut(s) 481, 892
TaaI ACNGT 1 cut(s) 50
TaiI ACGT 1 cut(s) 449
TaqI TCGA 4 cut(s) 154, 237, 443, 449
TasI AATT 2 cut(s) 158, 584
TatI WGTACW 2 cut(s) 13, 23
TfiI GAWTC 1 cut(s) 790
Tru1I TTAA 2 cut(s) 303, 611
Tru9I TTAA 2 cut(s) 303, 611
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 2 cut(s) 229, 754
Tsp45I GTSAC 2 cut(s) 229, 754
TspDTI ATGAA 1 cut(s) 138
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 1 cut(s) 67
Van91I CCANNNNNTGG 1 cut(s) 847
VpaK11BI GGWCC 1 cut(s) 242
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 1 cut(s) 158
XceI RCATGY 2 cut(s) 20, 628
XmnI GAANNNNTTC 1 cut(s) 714
XspI CTAG 2 cut(s) 722, 797
ZraI GACGTC 1 cut(s) 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.