FvH4_2g33371

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000710
Physical Location & Seq
Forward (+)
25051536 .. 25052639
1104 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g33371.t1

Sequence Viewer

Length: 369 bp
ATGCCGAGCGAGGCAGCTGGGATCATTGGCTGTTTAAACTCTCTTCGAAACCCCCTTGTTGCAGCAGCTTTCAACGTTGGATCAACCACTGTCCTTGTAGCTGAAGCTCTCGCTCTTCGTAATAGCCTCATTGAAGCAAAAAGGAGAGGCTTTACTAAGGTGGAGATAGAAGGTGACTCCAAGTTTGTGTTAGATGCCATTAATGGCCACTCTACACCCTCTTGGAGAATTCACAAGCTTTATCAAGATATCAAGGCTCTTCGAAGCTCGTTTGAGTTTGTTAGTTTCAAGCATGTGTTTAGAGAGGCAAACTTTGTGGTAAATGCCTTTGCCAACCTTGGTCATAGGTTAGAGAACCATCGTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

123

Amino Acids

13.52

Weight (kDa)

9.65

Isoelectric Point (pI)

45.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 26 - 113 1.7e-19 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 75
AclWI GGATC 2 cut(s) 29, 88
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 1 cut(s) 228
AcuI CTGAAG 1 cut(s) 123
AgsI TTSAA 3 cut(s) 73, 134, 289
AluBI AGCT 6 cut(s) 17, 68, 101, 107, 238, 267
AluI AGCT 6 cut(s) 17, 68, 101, 107, 238, 267
AlwI GGATC 2 cut(s) 29, 88
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 3 cut(s) 14, 62, 65
ApoI RAATTY 1 cut(s) 228
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AseI ATTAAT 1 cut(s) 201
AsuHPI GGTGA 1 cut(s) 185
AsuII TTCGAA 2 cut(s) 46, 262
BalI TGGCCA 1 cut(s) 207
BbvI GCAGC 3 cut(s) 26, 74, 77
BccI CCATC 1 cut(s) 366
BfaI CTAG 1 cut(s) 367
BisI GCNGC 3 cut(s) 15, 63, 66
BlsI GCNGC 3 cut(s) 16, 64, 67
BmsI GCATC 1 cut(s) 184
Bpu14I TTCGAA 2 cut(s) 46, 262
BsaJI CCNNGG 1 cut(s) 337
BseDI CCNNGG 1 cut(s) 337
BseXI GCAGC 3 cut(s) 26, 74, 77
BseYI CCCAGC 1 cut(s) 17
BshFI GGCC 1 cut(s) 207
BsnI GGCC 1 cut(s) 207
Bsp119I TTCGAA 2 cut(s) 46, 262
Bsp143I GATC 2 cut(s) 21, 80
BspANI GGCC 1 cut(s) 207
BspPI GGATC 2 cut(s) 29, 88
BspQI GCTCTTC 2 cut(s) 120, 264
BspT104I TTCGAA 2 cut(s) 46, 262
BssECI CCNNGG 1 cut(s) 337
BssMI GATC 2 cut(s) 21, 80
BssT1I CCWWGG 1 cut(s) 337
Bst4CI ACNGT 1 cut(s) 91
Bst6I CTCTTC 3 cut(s) 48, 120, 264
BstBI TTCGAA 2 cut(s) 46, 262
BstDEI CTNAG 1 cut(s) 156
BstKTI GATC 2 cut(s) 24, 83
BstMBI GATC 2 cut(s) 21, 80
BstNSI RCATGY 1 cut(s) 296
BstV1I GCAGC 3 cut(s) 26, 74, 77
BsuRI GGCC 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 87
CspCI CAANNNNNGTGG 4 cut(s) 76, 111, 297, 332
CviAII CATG 1 cut(s) 293
DdeI CTNAG 1 cut(s) 156
DpnI GATC 2 cut(s) 23, 82
DpnII GATC 2 cut(s) 21, 80
DraI TTTAAA 1 cut(s) 36
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 3 cut(s) 48, 120, 264
EarI CTCTTC 3 cut(s) 48, 120, 264
Eco130I CCWWGG 1 cut(s) 337
Eco32I GATATC 1 cut(s) 250
Eco57I CTGAAG 1 cut(s) 123
EcoRI GAATTC 1 cut(s) 228
EcoRV GATATC 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 337
FaeI CATG 1 cut(s) 296
FaiI YATR 2 cut(s) 294, 345
FatI CATG 1 cut(s) 292
Fnu4HI GCNGC 3 cut(s) 15, 63, 66
Fsp4HI GCNGC 3 cut(s) 15, 63, 66
FspBI CTAG 1 cut(s) 367
GluI GCNGC 3 cut(s) 15, 63, 66
GsaI CCCAGC 1 cut(s) 21
HaeIII GGCC 1 cut(s) 207
Hin1II CATG 1 cut(s) 296
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 1 cut(s) 176
HphI GGTGA 1 cut(s) 185
Hpy188III TCNNGA 1 cut(s) 245
HpyAV CCTTC 1 cut(s) 164
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 1 cut(s) 62
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 1 cut(s) 75
Hsp92II CATG 1 cut(s) 296
Kzo9I GATC 2 cut(s) 21, 80
LguI GCTCTTC 2 cut(s) 120, 264
LpnPI CCDG 1 cut(s) 3
Lsp1109I GCAGC 3 cut(s) 26, 74, 77
LweI GCATC 1 cut(s) 184
MaeI CTAG 1 cut(s) 367
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 1 cut(s) 173
MalI GATC 2 cut(s) 23, 82
MboI GATC 2 cut(s) 21, 80
MboII GAAGA 3 cut(s) 35, 107, 251
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 1 cut(s) 228
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 1 cut(s) 170
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 5 cut(s) 4, 137, 140, 229, 298
Mox20I TGGCCA 1 cut(s) 207
MscI TGGCCA 1 cut(s) 207
MseI TTAA 2 cut(s) 35, 201
Msp20I TGGCCA 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 17
MssI GTTTAAAC 1 cut(s) 36
NdeII GATC 2 cut(s) 21, 80
NlaIII CATG 1 cut(s) 296
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 173
NspI RCATGY 1 cut(s) 296
NspV TTCGAA 2 cut(s) 46, 262
PciSI GCTCTTC 2 cut(s) 120, 264
PkrI GCNGC 3 cut(s) 16, 64, 67
PleI GAGTC 1 cut(s) 170
PmeI GTTTAAAC 1 cut(s) 36
PpsI GAGTC 1 cut(s) 170
PshBI ATTAAT 1 cut(s) 201
Psp1406I AACGTT 1 cut(s) 75
PspFI CCCAGC 1 cut(s) 17
PvuII CAGCTG 1 cut(s) 17
SapI GCTCTTC 2 cut(s) 120, 264
SaqAI TTAA 2 cut(s) 35, 201
SatI GCNGC 3 cut(s) 15, 63, 66
Sau3AI GATC 2 cut(s) 21, 80
SchI GAGTC 1 cut(s) 170
SfaNI GCATC 1 cut(s) 184
SfuI TTCGAA 2 cut(s) 46, 262
Sse9I AATT 1 cut(s) 228
SspMI CTAG 1 cut(s) 367
StyI CCWWGG 1 cut(s) 337
TaaI ACNGT 1 cut(s) 91
TaiI ACGT 1 cut(s) 78
TaqI TCGA 2 cut(s) 46, 262
TasI AATT 1 cut(s) 228
Tru1I TTAA 2 cut(s) 35, 201
Tru9I TTAA 2 cut(s) 35, 201
TscAI CASTG 1 cut(s) 94
TseFI GTSAC 1 cut(s) 173
TseI GCWGC 3 cut(s) 14, 62, 65
Tsp45I GTSAC 1 cut(s) 173
TspRI CASTG 1 cut(s) 94
VspI ATTAAT 1 cut(s) 201
XapI RAATTY 1 cut(s) 228
XceI RCATGY 1 cut(s) 296
XspI CTAG 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.