FvH4_5g24522

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000710
Physical Location & Seq
Reverse (-)
15891100 .. 15896115
5016 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g24522.t1

Sequence Viewer

Length: 1074 bp
ATGTTGACTGATGAAATTGGCAAGCAAGGAAGAAATTTCTTATGGGGAACAGAGATGAAAACAGCTCCTGTAGCTTGGAAAGAAGTGTGTAAGCCAAAAATGTTGGGGGGACTGGTGGTCAGACCCTCAGCTTTCTTCAACAAGGCTGCAATTGCTAAATTAGCATGGAAATTAGTTACTGACAAGAACAACTGGTGGGTGCAGGTGGTCTCCAAGAAATATCTGAGGCGATGTTCCTTCTTCGATGCAAAGAAGAAGCATAAAAACTCAATGGCATGGAATGGCATCCTTGATTCAAGGGACCTTATTCTTAAAGGTATGAGATGGATAGTTGGAGATGTGGTTAAGGAGATCATGGGGGTTCATATACCAGCTAATCAGCTAGAAGATAACTTTATCTGGGGCCCTGCTCCTCATGGCAAGTTCACAGTTAAATCAGCAACTTGGATGCAACTCCAAGATACTCATGAACATAGCCGAAAATCTCTCGCTACATCATGCTTTAACCTTGGCACCACCACTGTTCTAGTTGCGGAAGCGATTGCCTTACGCAATAGCCTCATCTACACGAAGAATCAAGGCCTCGCCAAAATTGAAGTTGAAGGCGATTCTAAGCTGGTGATTGATGATCTGAATGGAGTTTCAAGTCCTCCTTGGAAACTACTTAAGCTTGTTCAAGATATTAAGGCTTTGAGTAGTTCCTTTGAGTTTGTTAAATTCAAGCATGTCTTTAGGGAAGCCAATTTCGTGGTTAATGTTTTGGCCAATCTTGGTCACAGGGTTGATAATATGAATTTGTGGGAGTGTGTTCCTCCAGAGTCTATGGATGAGCTTAGTGCCCCAGGTTCAGATGGTTGCGCAAGAACATCGTTGGACTGTAAGGGAGTCGTCGTTTATACCTCTGAGTCCTACGACGGCGTGATTGGTTGTATGGAGTTTCTTTGTTTCTTGGCGTCGACGACAAAGCTAGGCAGATCGAGGCAGATTGATGATTGTGGCGTGACTCTTGCTTTCATGTTAGGTCATGGTGGATCTGTCTCTTTAGGTGACAATAGGGTTGACAGCGAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

358

Amino Acids

39.73

Weight (kDa)

8.75

Isoelectric Point (pI)

35.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 170 - 257 8.5e-17 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 193
Acc16I TGCGCA 1 cut(s) 859
Acc36I ACCTGC 1 cut(s) 193
AccB1I GGYRCC 1 cut(s) 512
AccI GTMKAC 1 cut(s) 956
AciI CCGC 1 cut(s) 533
AclWI GGATC 1 cut(s) 1039
AcoI YGGCCR 1 cut(s) 762
AcsI RAATTY 4 cut(s) 34, 716, 793, 1067
AcyI GRCGYC 1 cut(s) 953
AflII CTTAAG 1 cut(s) 665
AgsI TTSAA 7 cut(s) 139, 297, 596, 602, 645, 677, 721
AhdI GACNNNNNGTC 1 cut(s) 116
AjnI CCWGG 1 cut(s) 841
AluBI AGCT 9 cut(s) 65, 74, 131, 374, 382, 616, 670, 832, 967
AluI AGCT 9 cut(s) 65, 74, 131, 374, 382, 616, 670, 832, 967
Alw26I GTCTC 2 cut(s) 214, 1042
AlwI GGATC 1 cut(s) 1039
AlwNI CAGNNNCTG 1 cut(s) 68
AoxI GGCC 3 cut(s) 403, 580, 762
ApaI GGGCCC 1 cut(s) 407
ApeKI GCWGC 1 cut(s) 146
ApoI RAATTY 4 cut(s) 34, 716, 793, 1067
AspLEI GCGC 1 cut(s) 860
AspS9I GGNCC 3 cut(s) 301, 403, 404
AsuHPI GGTGA 2 cut(s) 631, 1058
AvaII GGWCC 1 cut(s) 301
BaeGI GKGCMC 2 cut(s) 407, 841
BalI TGGCCA 1 cut(s) 764
BanI GGYRCC 1 cut(s) 512
BanII GRGCYC 1 cut(s) 407
BbvCI CCTCAGC 1 cut(s) 127
BbvI GCAGC 1 cut(s) 133
BccI CCATC 2 cut(s) 318, 845
BceAI ACGGC 1 cut(s) 931
BcgI CGANNNNNNTGC 2 cut(s) 849, 883
BciT130I CCWGG 1 cut(s) 843
BcoDI GTCTC 2 cut(s) 214, 1042
BfaI CTAG 3 cut(s) 383, 527, 968
BfmI CTRYAG 1 cut(s) 69
BfrI CTTAAG 1 cut(s) 665
BfuAI ACCTGC 1 cut(s) 193
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
Bme1390I CCNGG 1 cut(s) 843
Bme18I GGWCC 1 cut(s) 301
BmeRI GACNNNNNGTC 1 cut(s) 116
BmgT120I GGNCC 3 cut(s) 301, 403, 404
BmiI GGNNCC 4 cut(s) 302, 404, 405, 514
BmrFI CCNGG 1 cut(s) 843
BmsI GCATC 3 cut(s) 235, 294, 438
BpmI CTGGAG 1 cut(s) 798
Bpu10I CCTNAGC 1 cut(s) 127
BsaHI GRCGYC 1 cut(s) 953
BsaI GGTCTC 1 cut(s) 214
BsaJI CCNNGG 3 cut(s) 508, 653, 841
BsaXI ACNNNNNCTCC 4 cut(s) 327, 357, 876, 906
Bse1I ACTGG 2 cut(s) 117, 197
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 3 cut(s) 508, 653, 841
BseGI GGATG 3 cut(s) 285, 453, 832
BseMII CTCAG 3 cut(s) 141, 215, 894
BseNI ACTGG 2 cut(s) 117, 197
BseRI GAGGAG 1 cut(s) 402
BseSI GKGCMC 2 cut(s) 407, 841
BseXI GCAGC 1 cut(s) 133
BsgI GTGCAG 1 cut(s) 221
BshFI GGCC 3 cut(s) 405, 582, 764
BshNI GGYRCC 1 cut(s) 512
BslFI GGGAC 2 cut(s) 123, 314
BsmAI GTCTC 2 cut(s) 214, 1042
BsmFI GGGAC 2 cut(s) 123, 314
BsnI GGCC 3 cut(s) 405, 582, 764
Bso31I GGTCTC 1 cut(s) 214
Bsp120I GGGCCC 1 cut(s) 403
Bsp1286I GDGCHC 2 cut(s) 407, 841
Bsp143I GATC 4 cut(s) 351, 628, 974, 1031
BspACI CCGC 1 cut(s) 533
BspANI GGCC 3 cut(s) 405, 582, 764
BspCNI CTCAG 3 cut(s) 140, 216, 895
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 4 cut(s) 302, 404, 405, 514
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 1 cut(s) 1039
BspT107I GGYRCC 1 cut(s) 512
BspTI CTTAAG 1 cut(s) 665
BspTNI GGTCTC 1 cut(s) 214
BsrI ACTGG 2 cut(s) 117, 197
BssECI CCNNGG 3 cut(s) 508, 653, 841
BssMI GATC 4 cut(s) 351, 628, 974, 1031
BssNI GRCGYC 1 cut(s) 953
BssT1I CCWWGG 2 cut(s) 508, 653
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 3 cut(s) 430, 523, 878
BstACI GRCGYC 1 cut(s) 953
BstAFI CTTAAG 1 cut(s) 665
BstC8I GCNNGC 1 cut(s) 23
BstDEI CTNAG 5 cut(s) 127, 224, 612, 833, 903
BstF5I GGATG 3 cut(s) 285, 453, 832
BstHHI GCGC 1 cut(s) 860
BstKTI GATC 4 cut(s) 354, 631, 977, 1034
BstMAI GTCTC 2 cut(s) 214, 1042
BstMBI GATC 4 cut(s) 351, 628, 974, 1031
BstMWI GCNNNNNNNGC 3 cut(s) 71, 152, 161
BstNI CCWGG 1 cut(s) 843
BstNSI RCATGY 1 cut(s) 728
BstSCI CCNGG 1 cut(s) 841
BstSFI CTRYAG 1 cut(s) 69
BstSLI GKGCMC 2 cut(s) 407, 841
BstV1I GCAGC 1 cut(s) 133
BstX2I RGATCY 1 cut(s) 1031
BstXI CCANNNNNNTGG 1 cut(s) 748
BstYI RGATCY 1 cut(s) 1031
BsuRI GGCC 3 cut(s) 405, 582, 764
BtgZI GCGATG 1 cut(s) 244
BtsCI GGATG 3 cut(s) 285, 453, 832
BtsIMutI CAGTG 1 cut(s) 519
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 1 cut(s) 23
CaiI CAGNNNCTG 1 cut(s) 68
CciI TCATGA 1 cut(s) 466
CfoI GCGC 1 cut(s) 860
Cfr13I GGNCC 3 cut(s) 301, 403, 404
CseI GACGC 1 cut(s) 942
CviAII CATG 9 cut(s) 165, 276, 355, 416, 467, 498, 725, 1015, 1025
DdeI CTNAG 5 cut(s) 127, 224, 612, 833, 903
DpnI GATC 4 cut(s) 353, 630, 976, 1033
DpnII GATC 4 cut(s) 351, 628, 974, 1031
DriI GACNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 1 cut(s) 762
Eam1105I GACNNNNNGTC 1 cut(s) 116
Eco130I CCWWGG 2 cut(s) 508, 653
Eco147I AGGCCT 1 cut(s) 582
Eco24I GRGCYC 1 cut(s) 407
Eco31I GGTCTC 1 cut(s) 214
Eco47I GGWCC 1 cut(s) 301
EcoO109I RGGNCCY 3 cut(s) 301, 403, 404
EcoRII CCWGG 1 cut(s) 841
EcoT14I CCWWGG 2 cut(s) 508, 653
EcoT38I GRGCYC 1 cut(s) 407
ErhI CCWWGG 2 cut(s) 508, 653
FaeI CATG 9 cut(s) 168, 279, 358, 419, 470, 501, 728, 1018, 1028
FalI AAGNNNNNCTT 4 cut(s) 637, 669, 713, 745
FaqI GGGAC 2 cut(s) 123, 314
FatI CATG 9 cut(s) 164, 275, 354, 415, 466, 497, 724, 1014, 1024
FblI GTMKAC 1 cut(s) 956
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 3 cut(s) 272, 460, 839
FriOI GRGCYC 1 cut(s) 407
Fsp4HI GCNGC 1 cut(s) 147
FspBI CTAG 3 cut(s) 383, 527, 968
FspI TGCGCA 1 cut(s) 859
GlaI GCGC 1 cut(s) 859
GluI GCNGC 1 cut(s) 147
GsuI CTGGAG 1 cut(s) 798
HaeIII GGCC 3 cut(s) 405, 582, 764
HgaI GACGC 1 cut(s) 942
HhaI GCGC 1 cut(s) 860
Hin1I GRCGYC 1 cut(s) 953
Hin1II CATG 9 cut(s) 168, 279, 358, 419, 470, 501, 728, 1018, 1028
Hin6I GCGC 1 cut(s) 858
HinP1I GCGC 1 cut(s) 858
HincII GTYRAC 3 cut(s) 6, 957, 1060
HindII GTYRAC 3 cut(s) 6, 957, 1060
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 7 cut(s) 293, 574, 608, 818, 885, 905, 1003
HphI GGTGA 2 cut(s) 631, 1058
Hpy166II GTNNAC 4 cut(s) 6, 426, 957, 1060
Hpy188I TCNGA 5 cut(s) 122, 225, 633, 850, 904
Hpy188III TCNNGA 3 cut(s) 467, 677, 815
Hpy8I GTNNAC 4 cut(s) 6, 426, 957, 1060
Hpy99I CGWCG 4 cut(s) 893, 917, 958, 961
HpyAV CCTTC 2 cut(s) 247, 596
HpyCH4III ACNGT 3 cut(s) 430, 523, 878
HpyCH4V TGCA 4 cut(s) 149, 202, 248, 451
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 152, 161
HpyF3I CTNAG 5 cut(s) 127, 224, 612, 833, 903
Hsp92I GRCGYC 1 cut(s) 953
Hsp92II CATG 9 cut(s) 168, 279, 358, 419, 470, 501, 728, 1018, 1028
HspAI GCGC 1 cut(s) 858
Kzo9I GATC 4 cut(s) 351, 628, 974, 1031
LmnI GCTCC 2 cut(s) 70, 415
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 3 cut(s) 235, 294, 438
MaeI CTAG 3 cut(s) 383, 527, 968
MaeIII GTNAC 4 cut(s) 175, 773, 1000, 1046
MalI GATC 4 cut(s) 353, 630, 976, 1033
MboI GATC 4 cut(s) 351, 628, 974, 1031
MboII GAAGA 6 cut(s) 42, 127, 232, 265, 398, 583
MfeI CAATTG 1 cut(s) 150
MflI RGATCY 1 cut(s) 1031
MhlI GDGCHC 2 cut(s) 407, 841
MlsI TGGCCA 1 cut(s) 764
MluNI TGGCCA 1 cut(s) 764
MlyI GAGTC 4 cut(s) 827, 894, 914, 997
MmeI TCCRAC 2 cut(s) 313, 852
MnlI CCTC 9 cut(s) 136, 219, 423, 569, 593, 660, 822, 910, 972
Mox20I TGGCCA 1 cut(s) 764
MscI TGGCCA 1 cut(s) 764
MseI TTAA 8 cut(s) 312, 345, 432, 504, 666, 684, 714, 753
Msp20I TGGCCA 1 cut(s) 764
MspCI CTTAAG 1 cut(s) 665
MspR9I CCNGG 1 cut(s) 843
MunI CAATTG 1 cut(s) 150
MvaI CCWGG 1 cut(s) 843
MwoI GCNNNNNNNGC 3 cut(s) 71, 152, 161
NdeII GATC 4 cut(s) 351, 628, 974, 1031
NlaIII CATG 9 cut(s) 168, 279, 358, 419, 470, 501, 728, 1018, 1028
NlaIV GGNNCC 4 cut(s) 302, 404, 405, 514
NmuCI GTSAC 3 cut(s) 773, 1000, 1046
NsbI TGCGCA 1 cut(s) 859
NspI RCATGY 1 cut(s) 728
PagI TCATGA 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 193
PceI AGGCCT 1 cut(s) 582
PfeI GAWTC 3 cut(s) 293, 574, 608
PkrI GCNGC 1 cut(s) 148
PleI GAGTC 4 cut(s) 826, 893, 913, 997
PpsI GAGTC 4 cut(s) 826, 893, 913, 997
PpuMI RGGWCCY 1 cut(s) 301
Psp5II RGGWCCY 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 841
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 4 cut(s) 302, 404, 405, 514
PspOMI GGGCCC 1 cut(s) 403
PspPI GGNCC 3 cut(s) 301, 403, 404
PspPPI RGGWCCY 1 cut(s) 301
PstNI CAGNNNCTG 1 cut(s) 68
PsuI RGATCY 1 cut(s) 1031
SalI GTCGAC 1 cut(s) 955
SaqAI TTAA 8 cut(s) 312, 345, 432, 504, 666, 684, 714, 753
SatI GCNGC 1 cut(s) 147
Sau3AI GATC 4 cut(s) 351, 628, 974, 1031
Sau96I GGNCC 3 cut(s) 301, 403, 404
SchI GAGTC 4 cut(s) 827, 894, 914, 997
ScrFI CCNGG 1 cut(s) 843
SduI GDGCHC 2 cut(s) 407, 841
SfaNI GCATC 3 cut(s) 235, 294, 438
SfcI CTRYAG 1 cut(s) 69
SgrDI CGTCGACG 1 cut(s) 955
SinI GGWCC 1 cut(s) 301
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
SseBI AGGCCT 1 cut(s) 582
SsiI CCGC 1 cut(s) 533
SspMI CTAG 3 cut(s) 383, 527, 968
StuI AGGCCT 1 cut(s) 582
StyD4I CCNGG 1 cut(s) 841
StyI CCWWGG 2 cut(s) 508, 653
TaaI ACNGT 3 cut(s) 430, 523, 878
TaqI TCGA 3 cut(s) 243, 956, 977
TfiI GAWTC 3 cut(s) 293, 574, 608
Tru1I TTAA 8 cut(s) 312, 345, 432, 504, 666, 684, 714, 753
Tru9I TTAA 8 cut(s) 312, 345, 432, 504, 666, 684, 714, 753
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 3 cut(s) 773, 1000, 1046
TseI GCWGC 1 cut(s) 146
Tsp45I GTSAC 3 cut(s) 773, 1000, 1046
TspDTI ATGAA 6 cut(s) 27, 71, 353, 483, 806, 1003
TspRI CASTG 1 cut(s) 526
Vha464I CTTAAG 1 cut(s) 665
VpaK11BI GGWCC 1 cut(s) 301
XapI RAATTY 4 cut(s) 34, 716, 793, 1067
XceI RCATGY 1 cut(s) 728
XmiI GTMKAC 1 cut(s) 956
XspI CTAG 3 cut(s) 383, 527, 968
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.