Rorug03G0234400

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000710
Physical Location & Seq
Reverse (-)
21677732 .. 21678681
950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0234400.1

Sequence Viewer

Length: 264 bp
ATGGTTCGTTGCCTCATACTTGAATCATTTGCATATTCCTGCTGTTGGATTTTCTGGACAGTCGGAATCTTGGCATCCTCAACTGACCGCGGTCGTGCACACGCGATAAGGATCCCCGAGCACTCATGTCATAAGCAGATTGAGTCAAGCAAATGTGAGCTCCAGAAATGCATTCAGGAATGCTCCAAAGAACCATCTGGCGCAGGAGCGTGCAAAAATACCATTTGCTTTTGCACATACTATTGCAAGGATCCTCCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

87

Amino Acids

9.8

Weight (kDa)

8.01

Isoelectric Point (pI)

69.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SLR1-BP PF07333 41 - 82 8.2e-06 S locus-related glycoprotein 1 binding pollen coat protein (SLR1-BP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 90, 104
AciI CCGC 2 cut(s) 88, 90
AclWI GGATC 4 cut(s) 106, 119, 245, 258
AfiI CCNNNNNNNGG 1 cut(s) 45
AgsI TTSAA 1 cut(s) 23
AluBI AGCT 1 cut(s) 160
AluI AGCT 1 cut(s) 160
Alw21I GWGCWC 3 cut(s) 100, 123, 162
Alw44I GTGCAC 1 cut(s) 96
AlwI GGATC 4 cut(s) 106, 119, 245, 258
Ama87I CYCGRG 1 cut(s) 116
ApaLI GTGCAC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 203
AvaI CYCGRG 1 cut(s) 116
BaeGI GKGCMC 1 cut(s) 100
BamHI GGATCC 2 cut(s) 111, 250
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 3 cut(s) 100, 123, 162
BccI CCATC 1 cut(s) 202
BmeT110I CYCGRG 1 cut(s) 116
BmiI GGNNCC 2 cut(s) 113, 252
BmsI GCATC 1 cut(s) 83
BoxI GACNNNNGTC 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 146
BsaBI GATNNNNATC 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 88
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse8I GATNNNNATC 1 cut(s) 110
BseDI CCNNGG 1 cut(s) 88
BseGI GGATG 1 cut(s) 74
BseJI GATNNNNATC 1 cut(s) 110
BseLI CCNNNNNNNGG 1 cut(s) 45
BseSI GKGCMC 1 cut(s) 100
Bsh1236I CGCG 2 cut(s) 90, 104
Bsh1285I CGRYCG 1 cut(s) 94
BsiEI CGRYCG 1 cut(s) 94
BsiHKAI GWGCWC 3 cut(s) 100, 123, 162
BsiHKCI CYCGRG 1 cut(s) 116
BslI CCNNNNNNNGG 1 cut(s) 45
BsmI GAATGC 2 cut(s) 171, 185
BsoBI CYCGRG 1 cut(s) 116
Bsp1286I GDGCHC 3 cut(s) 100, 123, 162
Bsp143I GATC 2 cut(s) 111, 250
BspACI CCGC 2 cut(s) 88, 90
BspFNI CGCG 2 cut(s) 90, 104
BspLI GGNNCC 2 cut(s) 113, 252
BspPI GGATC 4 cut(s) 106, 119, 245, 258
BssECI CCNNGG 1 cut(s) 88
BssMI GATC 2 cut(s) 111, 250
Bst4CI ACNGT 1 cut(s) 61
BstC8I GCNNGC 1 cut(s) 211
BstDSI CCRYGG 1 cut(s) 88
BstF5I GGATG 1 cut(s) 74
BstFNI CGCG 2 cut(s) 90, 104
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 2 cut(s) 114, 253
BstMBI GATC 2 cut(s) 111, 250
BstMCI CGRYCG 1 cut(s) 94
BstPAI GACNNNNGTC 1 cut(s) 90
BstSLI GKGCMC 1 cut(s) 100
BstUI CGCG 2 cut(s) 90, 104
BstX2I RGATCY 2 cut(s) 111, 250
BstYI RGATCY 2 cut(s) 111, 250
BtgI CCRYGG 1 cut(s) 88
BtsCI GGATG 1 cut(s) 74
Cac8I GCNNGC 1 cut(s) 211
CfoI GCGC 1 cut(s) 203
Cfr42I CCGCGG 1 cut(s) 91
CviAII CATG 1 cut(s) 126
CviJI RGCY 1 cut(s) 160
CviKI_1 RGCY 1 cut(s) 160
DpnI GATC 2 cut(s) 113, 252
DpnII GATC 2 cut(s) 111, 250
Ecl136II GAGCTC 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 162
Eco53kI GAGCTC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 116
EcoICRI GAGCTC 1 cut(s) 160
EcoT22I ATGCAT 1 cut(s) 173
EcoT38I GRGCYC 1 cut(s) 162
FaeI CATG 1 cut(s) 129
FaiI YATR 5 cut(s) 17, 34, 127, 132, 238
FatI CATG 1 cut(s) 125
FokI GGATG 1 cut(s) 61
FriOI GRGCYC 1 cut(s) 162
GlaI GCGC 1 cut(s) 202
GsuI CTGGAG 1 cut(s) 146
HhaI GCGC 1 cut(s) 203
Hin1II CATG 1 cut(s) 129
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HinfI GANTC 3 cut(s) 23, 66, 143
Hpy166II GTNNAC 1 cut(s) 98
Hpy188I TCNGA 1 cut(s) 65
Hpy188III TCNNGA 3 cut(s) 55, 163, 176
Hpy8I GTNNAC 1 cut(s) 98
HpyCH4III ACNGT 1 cut(s) 61
HpyCH4V TGCA 6 cut(s) 32, 98, 171, 213, 234, 246
Hsp92II CATG 1 cut(s) 129
HspAI GCGC 1 cut(s) 201
KspI CCGCGG 1 cut(s) 91
Kzo9I GATC 2 cut(s) 111, 250
LmnI GCTCC 3 cut(s) 165, 188, 206
LpnPI CCDG 6 cut(s) 40, 52, 161, 176, 183, 189
LweI GCATC 1 cut(s) 83
MalI GATC 2 cut(s) 113, 252
MboI GATC 2 cut(s) 111, 250
MflI RGATCY 2 cut(s) 111, 250
MhlI GDGCHC 3 cut(s) 100, 123, 162
MluCI AATT 1 cut(s) 259
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 2 cut(s) 26, 43
MnlI CCTC 3 cut(s) 23, 88, 264
Mph1103I ATGCAT 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 90
Mva1269I GAATGC 2 cut(s) 171, 185
MvnI CGCG 2 cut(s) 90, 104
NdeII GATC 2 cut(s) 111, 250
NlaIII CATG 1 cut(s) 129
NlaIV GGNNCC 2 cut(s) 113, 252
NsiI ATGCAT 1 cut(s) 173
PctI GAATGC 2 cut(s) 171, 185
PfeI GAWTC 2 cut(s) 23, 66
PleI GAGTC 1 cut(s) 151
PpsI GAGTC 1 cut(s) 151
PshAI GACNNNNGTC 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 113, 252
PsuI RGATCY 2 cut(s) 111, 250
SacI GAGCTC 1 cut(s) 162
SacII CCGCGG 1 cut(s) 91
Sau3AI GATC 2 cut(s) 111, 250
SchI GAGTC 1 cut(s) 152
SduI GDGCHC 3 cut(s) 100, 123, 162
SetI ASST 1 cut(s) 162
SfaNI GCATC 1 cut(s) 83
Sfr303I CCGCGG 1 cut(s) 91
SgrBI CCGCGG 1 cut(s) 91
Sse9I AATT 1 cut(s) 259
SsiI CCGC 2 cut(s) 88, 90
SstI GAGCTC 1 cut(s) 162
TaaI ACNGT 1 cut(s) 61
TasI AATT 1 cut(s) 259
TfiI GAWTC 2 cut(s) 23, 66
VneI GTGCAC 1 cut(s) 96
Zsp2I ATGCAT 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.