FvH4_6g21761

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000710
Physical Location & Seq
Forward (+)
15300888 .. 15301550
663 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g21761.t1

Sequence Viewer

Length: 474 bp
ATGGTCCTTTTGATGCTTCTAGATTTGAGAAGACTTGTGCTGCAATTTACCATTGTCCGGCTTCAGCCTGCATCAATACCCCTTCAAGAAGTACAAGGGCCAACTCAAGCAACATTAAGTGGAGGCCCCTTTCCTGTGGAAGAATCAAGATCAATTTTGACGCTCGGTTATTCACAAAGCTCTAACGCTGCTGCTGGTTTTATATTGAGAGATGATTCTGGGTGCCATTTGGTTGCCTCTGCTCGAAAAATTGGGAAGACAACTGTTCCCAATGCAGAGGCCACGTCCCTCAGAGACAGTCTTATTAAAGCTAAAGATATGGGGATCCAAGATTTGGTAGTGGAAGGCATAAGCTTTCAACATATTCTTCGTGAAGCTAATTTTGCTGCTAACTTTGGCTATCCTATGCCACCAGCTTTCGAGTCCGCACTACTGGGAGGTGGGGTTTCCCCCGGCTGTGAGTTCGGCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

16.75

Weight (kDa)

5.63

Isoelectric Point (pI)

62.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 58 - 116 2.1e-07 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 222
AccB7I CCANNNNNTGG 1 cut(s) 334
AciI CCGC 1 cut(s) 426
AclWI GGATC 2 cut(s) 319, 332
AcuI CTGAAG 1 cut(s) 47
AfaI GTAC 1 cut(s) 93
AfiI CCNNNNNNNGG 2 cut(s) 57, 334
AgsI TTSAA 2 cut(s) 86, 359
AjiI CACGTC 1 cut(s) 285
AluBI AGCT 5 cut(s) 180, 311, 354, 377, 416
AluI AGCT 5 cut(s) 180, 311, 354, 377, 416
Alw26I GTCTC 1 cut(s) 288
AlwI GGATC 2 cut(s) 319, 332
AoxI GGCC 3 cut(s) 98, 124, 279
ApeKI GCWGC 4 cut(s) 40, 188, 191, 386
AspS9I GGNCC 3 cut(s) 4, 98, 125
AsuC2I CCSGG 1 cut(s) 453
AvaII GGWCC 1 cut(s) 4
BamHI GGATCC 1 cut(s) 324
BanI GGYRCC 1 cut(s) 222
BbsI GAAGAC 2 cut(s) 37, 263
BbvI GCAGC 4 cut(s) 27, 175, 178, 373
BcnI CCSGG 1 cut(s) 453
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 20
BisI GCNGC 4 cut(s) 41, 189, 192, 387
BlsI GCNGC 4 cut(s) 42, 190, 193, 388
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 1 cut(s) 4
BmgBI CACGTC 1 cut(s) 285
BmgT120I GGNCC 3 cut(s) 4, 98, 125
BmiI GGNNCC 3 cut(s) 127, 224, 326
BmrFI CCNGG 1 cut(s) 453
BmrI ACTGGG 1 cut(s) 443
BmsI GCATC 2 cut(s) 3, 80
BmuI ACTGGG 1 cut(s) 443
BpiI GAAGAC 2 cut(s) 37, 263
BpuEI CTTGAG 1 cut(s) 90
BpuMI CCSGG 1 cut(s) 453
BsaJI CCNNGG 1 cut(s) 451
BsaXI ACNNNNNCTCC 2 cut(s) 429, 459
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 334
Bse1I ACTGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 451
BseLI CCNNNNNNNGG 2 cut(s) 57, 334
BseMII CTCAG 1 cut(s) 304
BseNI ACTGG 1 cut(s) 438
BseXI GCAGC 4 cut(s) 27, 175, 178, 373
BshFI GGCC 3 cut(s) 100, 126, 281
BshNI GGYRCC 1 cut(s) 222
BsiSI CCGG 2 cut(s) 58, 453
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 2 cut(s) 57, 334
BsmAI GTCTC 1 cut(s) 288
BsmFI GGGAC 1 cut(s) 271
BsnI GGCC 3 cut(s) 100, 126, 281
Bsp143I GATC 2 cut(s) 149, 324
BspACI CCGC 1 cut(s) 426
BspANI GGCC 3 cut(s) 100, 126, 281
BspCNI CTCAG 1 cut(s) 303
BspLI GGNNCC 3 cut(s) 127, 224, 326
BspPI GGATC 2 cut(s) 319, 332
BspT107I GGYRCC 1 cut(s) 222
BsrI ACTGG 1 cut(s) 438
BssECI CCNNGG 1 cut(s) 451
BssMI GATC 2 cut(s) 149, 324
Bst4CI ACNGT 2 cut(s) 265, 299
BstC8I GCNNGC 1 cut(s) 69
BstDEI CTNAG 1 cut(s) 290
BstKTI GATC 2 cut(s) 152, 327
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 2 cut(s) 149, 324
BstMWI GCNNNNNNNGC 1 cut(s) 383
BstSCI CCNGG 1 cut(s) 451
BstV1I GCAGC 4 cut(s) 27, 175, 178, 373
BstV2I GAAGAC 2 cut(s) 37, 263
BstX2I RGATCY 1 cut(s) 324
BstYI RGATCY 1 cut(s) 324
BsuRI GGCC 3 cut(s) 100, 126, 281
BtrI CACGTC 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 69
Cfr13I GGNCC 3 cut(s) 4, 98, 125
CseI GACGC 1 cut(s) 169
Csp6I GTAC 1 cut(s) 92
CviQI GTAC 1 cut(s) 92
DdeI CTNAG 1 cut(s) 290
DpnI GATC 2 cut(s) 151, 326
DpnII GATC 2 cut(s) 149, 324
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 125
FaiI YATR 5 cut(s) 203, 320, 350, 363, 407
FaqI GGGAC 1 cut(s) 271
Fnu4HI GCNGC 4 cut(s) 41, 189, 192, 387
Fsp4HI GCNGC 4 cut(s) 41, 189, 192, 387
FspBI CTAG 1 cut(s) 20
GluI GCNGC 4 cut(s) 41, 189, 192, 387
HaeIII GGCC 3 cut(s) 100, 126, 281
HapII CCGG 2 cut(s) 58, 453
HgaI GACGC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 352
HinfI GANTC 3 cut(s) 143, 215, 422
HpaII CCGG 2 cut(s) 58, 453
Hpy188I TCNGA 1 cut(s) 293
Hpy188III TCNNGA 4 cut(s) 20, 86, 147, 371
HpyAV CCTTC 2 cut(s) 92, 338
HpyCH4III ACNGT 2 cut(s) 265, 299
HpyCH4IV ACGT 1 cut(s) 284
HpyCH4V TGCA 3 cut(s) 43, 71, 275
HpyF10VI GCNNNNNNNGC 1 cut(s) 383
HpyF3I CTNAG 1 cut(s) 290
HpySE526I ACGT 1 cut(s) 284
Kzo9I GATC 2 cut(s) 149, 324
LpnPI CCDG 8 cut(s) 71, 81, 147, 180, 204, 419, 426, 466
Lsp1109I GCAGC 4 cut(s) 27, 175, 178, 373
LweI GCATC 2 cut(s) 3, 80
MaeI CTAG 1 cut(s) 20
MaeII ACGT 1 cut(s) 284
MalI GATC 2 cut(s) 151, 326
MboI GATC 2 cut(s) 149, 324
MboII GAAGA 4 cut(s) 42, 152, 268, 359
MflI RGATCY 1 cut(s) 324
MluCI AATT 4 cut(s) 44, 153, 249, 379
MlyI GAGTC 1 cut(s) 431
MnlI CCTC 5 cut(s) 116, 247, 271, 299, 431
MseI TTAA 3 cut(s) 116, 306, 472
MspI CCGG 2 cut(s) 58, 453
MspR9I CCNGG 1 cut(s) 453
MwoI GCNNNNNNNGC 1 cut(s) 383
NciI CCSGG 1 cut(s) 453
NdeII GATC 2 cut(s) 149, 324
NlaIV GGNNCC 3 cut(s) 127, 224, 326
PfeI GAWTC 2 cut(s) 143, 215
PflMI CCANNNNNTGG 1 cut(s) 334
PkrI GCNGC 4 cut(s) 42, 190, 193, 388
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PspN4I GGNNCC 3 cut(s) 127, 224, 326
PspPI GGNCC 3 cut(s) 4, 98, 125
PsuI RGATCY 1 cut(s) 324
RsaI GTAC 1 cut(s) 93
RsaNI GTAC 1 cut(s) 92
SaqAI TTAA 3 cut(s) 116, 306, 472
SatI GCNGC 4 cut(s) 41, 189, 192, 387
Sau3AI GATC 2 cut(s) 149, 324
Sau96I GGNCC 3 cut(s) 4, 98, 125
SchI GAGTC 1 cut(s) 431
ScrFI CCNGG 1 cut(s) 453
SetI ASST 7 cut(s) 182, 287, 313, 356, 379, 418, 442
SfaNI GCATC 2 cut(s) 3, 80
SinI GGWCC 1 cut(s) 4
SmlI CTYRAG 1 cut(s) 105
SmoI CTYRAG 1 cut(s) 105
Sse9I AATT 4 cut(s) 44, 153, 249, 379
SsiI CCGC 1 cut(s) 426
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 1 cut(s) 451
TaaI ACNGT 2 cut(s) 265, 299
TaiI ACGT 1 cut(s) 287
TaqI TCGA 2 cut(s) 244, 420
TasI AATT 4 cut(s) 44, 153, 249, 379
TatI WGTACW 1 cut(s) 91
TfiI GAWTC 2 cut(s) 143, 215
Tru1I TTAA 3 cut(s) 116, 306, 472
Tru9I TTAA 3 cut(s) 116, 306, 472
TseI GCWGC 4 cut(s) 40, 188, 191, 386
Van91I CCANNNNNTGG 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 4
XbaI TCTAGA 1 cut(s) 19
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.