pycom05g07740

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000710
Physical Location & Seq
Forward (+)
10592642 .. 10592920
279 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g07740.1

Sequence Viewer

Length: 279 bp
ATGCAGCAAGTGCCTTTGTCATCAGATGCGTGTGTGCCGGGTGCTACGAAGATTGGTATTTCAGACATTCTTGTTGTTGACGGGCGGGCCTTAATCGATGCCACTGAGAGTATCCCAAAGATTGCTTGTCGGTTTCATAACGTGGAGTTTAAACATGTTTTTTGTGAAGCGAATTTCGTTTATGATGCTTTTGCTAAGATTGGTAAGGGATCCTCAAATTCTCATGTTTGGATGGTCTCTCTTCGTTTTGATACTCAAATCAACTTGGTTATGGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

93

Amino Acids

10.06

Weight (kDa)

6.24

Isoelectric Point (pI)

29.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 85
AclWI GGATC 2 cut(s) 204, 217
AcsI RAATTY 2 cut(s) 172, 217
AflIII ACRYGT 1 cut(s) 154
Alw26I GTCTC 1 cut(s) 241
AlwI GGATC 2 cut(s) 204, 217
AoxI GGCC 1 cut(s) 87
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 2 cut(s) 172, 217
AspS9I GGNCC 1 cut(s) 87
AsuC2I CCSGG 1 cut(s) 39
BamHI GGATCC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 16
BccI CCATC 1 cut(s) 226
BciVI GTATCC 1 cut(s) 122
BcnI CCSGG 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 241
BfuI GTATCC 1 cut(s) 122
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme1390I CCNGG 1 cut(s) 39
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 39
BmsI GCATC 3 cut(s) 16, 88, 175
BpuMI CCSGG 1 cut(s) 39
Bsa29I ATCGAT 1 cut(s) 96
BsaI GGTCTC 1 cut(s) 241
BseCI ATCGAT 1 cut(s) 96
BseGI GGATG 1 cut(s) 237
BseMII CTCAG 1 cut(s) 96
BseXI GCAGC 1 cut(s) 16
BshFI GGCC 1 cut(s) 89
BshVI ATCGAT 1 cut(s) 96
BsiSI CCGG 1 cut(s) 38
BsmAI GTCTC 1 cut(s) 241
BsnI GGCC 1 cut(s) 89
Bso31I GGTCTC 1 cut(s) 241
Bsp143I GATC 1 cut(s) 209
BspACI CCGC 1 cut(s) 85
BspANI GGCC 1 cut(s) 89
BspCNI CTCAG 1 cut(s) 97
BspDI ATCGAT 1 cut(s) 96
BspLI GGNNCC 1 cut(s) 211
BspPI GGATC 2 cut(s) 204, 217
BspTNI GGTCTC 1 cut(s) 241
BssMI GATC 1 cut(s) 209
Bst6I CTCTTC 1 cut(s) 246
BstAPI GCANNNNNTGC 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 87
BstDEI CTNAG 2 cut(s) 105, 195
BstF5I GGATG 1 cut(s) 237
BstKTI GATC 1 cut(s) 212
BstMAI GTCTC 1 cut(s) 241
BstMBI GATC 1 cut(s) 209
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 37
BstV1I GCAGC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 209
BstYI RGATCY 1 cut(s) 209
Bsu15I ATCGAT 1 cut(s) 96
BsuI GTATCC 1 cut(s) 122
BsuRI GGCC 1 cut(s) 89
BsuTUI ATCGAT 1 cut(s) 96
BtsCI GGATG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 102
Cac8I GCNNGC 1 cut(s) 87
Cfr13I GGNCC 1 cut(s) 87
ClaI ATCGAT 1 cut(s) 96
CviAII CATG 2 cut(s) 155, 224
CviJI RGCY 1 cut(s) 89
CviKI_1 RGCY 1 cut(s) 89
DdeI CTNAG 2 cut(s) 105, 195
DpnI GATC 1 cut(s) 211
DpnII GATC 1 cut(s) 209
DraI TTTAAA 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 241
FaeI CATG 2 cut(s) 158, 227
FaiI YATR 5 cut(s) 138, 156, 183, 225, 272
FatI CATG 2 cut(s) 154, 223
FauI CCCGC 1 cut(s) 78
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 1 cut(s) 244
Fsp4HI GCNGC 1 cut(s) 5
GluI GCNGC 1 cut(s) 5
HaeIII GGCC 1 cut(s) 89
HapII CCGG 1 cut(s) 38
Hin1II CATG 2 cut(s) 158, 227
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HpaII CCGG 1 cut(s) 38
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 2 cut(s) 25, 64
Hpy8I GTNNAC 1 cut(s) 79
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 2 cut(s) 105, 195
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 2 cut(s) 158, 227
Kzo9I GATC 1 cut(s) 209
LpnPI CCDG 1 cut(s) 51
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 16, 88, 175
MaeII ACGT 1 cut(s) 141
MalI GATC 1 cut(s) 211
MboI GATC 1 cut(s) 209
MboII GAAGA 2 cut(s) 61, 233
MflI RGATCY 1 cut(s) 209
MluCI AATT 2 cut(s) 172, 217
MnlI CCTC 1 cut(s) 223
MseI TTAA 2 cut(s) 92, 150
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 39
MssI GTTTAAAC 1 cut(s) 151
MwoI GCNNNNNNNGC 1 cut(s) 10
NciI CCSGG 1 cut(s) 39
NdeII GATC 1 cut(s) 209
NlaIII CATG 2 cut(s) 158, 227
NlaIV GGNNCC 1 cut(s) 211
NspI RCATGY 1 cut(s) 158
PciI ACATGT 1 cut(s) 154
PkrI GCNGC 1 cut(s) 6
PmeI GTTTAAAC 1 cut(s) 151
PscI ACATGT 1 cut(s) 154
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 87
PsuI RGATCY 1 cut(s) 209
SaqAI TTAA 2 cut(s) 92, 150
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 1 cut(s) 209
Sau96I GGNCC 1 cut(s) 87
ScrFI CCNGG 1 cut(s) 39
SetI ASST 1 cut(s) 144
SfaNI GCATC 3 cut(s) 16, 88, 175
Sse9I AATT 2 cut(s) 172, 217
SsiI CCGC 1 cut(s) 85
StyD4I CCNGG 1 cut(s) 37
TaiI ACGT 1 cut(s) 144
TaqI TCGA 1 cut(s) 96
TasI AATT 2 cut(s) 172, 217
Tru1I TTAA 2 cut(s) 92, 150
Tru9I TTAA 2 cut(s) 92, 150
TscAI CASTG 1 cut(s) 109
TseI GCWGC 1 cut(s) 4
TspDTI ATGAA 1 cut(s) 125
TspRI CASTG 1 cut(s) 109
XapI RAATTY 2 cut(s) 172, 217
XceI RCATGY 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.