FvH4_5g27791

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000710
Physical Location & Seq
Reverse (-)
18995355 .. 18996918
1564 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g27791.t1

Sequence Viewer

Length: 825 bp
ATGAATCCAAAAAAGAAAATAGGCATTGTAATCAAAGAGATTGAGGCTGGAAGGAAAGAGGATGCAGCCCCAATCGACAATGCTGCACTAAAAAATCGCTCGCCTGAACTGCAAGATATCAATAGTGGTACAGGATATTGTGGTATCAAATGGCAAACAAATGGTGTCACCAAGATAGAAGTGGAAAGCGACTTGAAGCTAGTGATCGATACTATGAATGAAGTTTCTACATCACCATGGAGGCTGCTCAAGTTAATCCAAGACATCAAGACATTGAGCAACTCGTTTCAGTTTGTGTGCTTTAAACATGTGTTTAGGGAAGCAAACTTTGTAGCCAATGCTTTGGCCAATTTAGGTCATAAGCTTAGCAATCTATGTTTTTGGGAGGAGAATATTCCGCCAGAAGCATCTTCTGCTTTGGCCTTTGACGATAATGAGGAAGAAGAGGATGTGGTGGTGGAAAAGTGCAGTGATTCGGGCTCATCCTCAACTGAAAAGAAAAAGGGTGACTCTGACTCATCTCAAATTGAAATGAAGAAGGGTGCGCAGATTGATGATGAAGTGAACCATGCCACCAAGCGTGTCTCACCACCATCTCTGGTCAAGATGAATTTAAAAAGGGAAGTTAAACGCCTCCTCGCCGAGATCGTCAATGCCGTAAGGTCCAACTACGTCAGGTGGAAAAGCACGTCGGTTGTTCGCGCTCGCTCGGTTCCATGTGGGAAATACCTTGCCGTCCTCACCGACAGCGTTAGAGGAGGAGGTCAAAATGATAGTTTTTTTTATTTGGAATCTTGTCCTCAGTTGGAAATGAGCAGTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

275

Amino Acids

30.39

Weight (kDa)

6.05

Isoelectric Point (pI)

53.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 52 - 118 1.8e-11 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 546
AccII CGCG 1 cut(s) 702
AciI CCGC 1 cut(s) 398
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 1 cut(s) 610
AfaI GTAC 1 cut(s) 130
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 2 cut(s) 196, 530
AjiI CACGTC 1 cut(s) 690
AluBI AGCT 2 cut(s) 199, 364
AluI AGCT 2 cut(s) 199, 364
Alw26I GTCTC 1 cut(s) 589
AoxI GGCC 2 cut(s) 345, 420
ApeKI GCWGC 3 cut(s) 65, 83, 244
ApoI RAATTY 1 cut(s) 610
AspLEI GCGC 2 cut(s) 547, 704
AspS9I GGNCC 1 cut(s) 663
AsuHPI GGTGA 5 cut(s) 160, 225, 518, 579, 733
AvaII GGWCC 1 cut(s) 663
BalI TGGCCA 1 cut(s) 347
BanII GRGCYC 1 cut(s) 482
BbvI GCAGC 3 cut(s) 70, 77, 231
BccI CCATC 1 cut(s) 601
BceAI ACGGC 2 cut(s) 641, 719
BcgI CGANNNNNNTGC 2 cut(s) 65, 99
BcoDI GTCTC 1 cut(s) 589
BfaI CTAG 1 cut(s) 200
BisI GCNGC 3 cut(s) 66, 84, 245
BlpI GCTNAGC 1 cut(s) 365
BlsI GCNGC 3 cut(s) 67, 85, 246
Bme18I GGWCC 1 cut(s) 663
BmgBI CACGTC 1 cut(s) 690
BmgT120I GGNCC 1 cut(s) 663
BmiI GGNNCC 1 cut(s) 714
BmsI GCATC 2 cut(s) 52, 416
Bpu1102I GCTNAGC 1 cut(s) 365
BpuEI CTTGAG 1 cut(s) 233
Bsa29I ATCGAT 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 236
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 236
BseGI GGATG 3 cut(s) 67, 454, 482
BseMII CTCAG 1 cut(s) 815
BseRI GAGGAG 4 cut(s) 401, 626, 771, 774
BseXI GCAGC 3 cut(s) 70, 77, 231
BsgI GTGCAG 2 cut(s) 69, 487
Bsh1236I CGCG 1 cut(s) 702
BshFI GGCC 2 cut(s) 347, 422
BshVI ATCGAT 1 cut(s) 207
BsmAI GTCTC 1 cut(s) 589
BsnI GGCC 2 cut(s) 347, 422
Bsp1286I GDGCHC 1 cut(s) 482
Bsp143I GATC 2 cut(s) 204, 645
Bsp1720I GCTNAGC 1 cut(s) 365
Bsp19I CCATGG 1 cut(s) 236
BspACI CCGC 1 cut(s) 398
BspANI GGCC 2 cut(s) 347, 422
BspCNI CTCAG 1 cut(s) 814
BspDI ATCGAT 1 cut(s) 207
BspFNI CGCG 1 cut(s) 702
BspLI GGNNCC 1 cut(s) 714
BssECI CCNNGG 1 cut(s) 236
BssMI GATC 2 cut(s) 204, 645
BssT1I CCWWGG 1 cut(s) 236
Bst6I CTCTTC 1 cut(s) 438
BstAPI GCANNNNNTGC 1 cut(s) 413
BstC8I GCNNGC 2 cut(s) 101, 706
BstDEI CTNAG 2 cut(s) 365, 801
BstDSI CCRYGG 1 cut(s) 236
BstF5I GGATG 3 cut(s) 67, 454, 482
BstFNI CGCG 1 cut(s) 702
BstHHI GCGC 2 cut(s) 547, 704
BstKTI GATC 2 cut(s) 207, 648
BstMAI GTCTC 1 cut(s) 589
BstMBI GATC 2 cut(s) 204, 645
BstMWI GCNNNNNNNGC 2 cut(s) 109, 413
BstNSI RCATGY 1 cut(s) 311
BstUI CGCG 1 cut(s) 702
BstV1I GCAGC 3 cut(s) 70, 77, 231
BstXI CCANNNNNNTGG 1 cut(s) 343
Bsu15I ATCGAT 1 cut(s) 207
BsuRI GGCC 2 cut(s) 347, 422
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 236
BtrI CACGTC 1 cut(s) 690
BtsCI GGATG 3 cut(s) 67, 454, 482
BtsI GCAGTG 2 cut(s) 475, 823
BtsIMutI CAGTG 2 cut(s) 475, 823
Cac8I GCNNGC 2 cut(s) 101, 706
CfoI GCGC 2 cut(s) 547, 704
Cfr13I GGNCC 1 cut(s) 663
ClaI ATCGAT 1 cut(s) 207
Csp6I GTAC 1 cut(s) 129
CviAII CATG 4 cut(s) 237, 308, 569, 717
CviJI RGCY 9 cut(s) 47, 68, 199, 244, 335, 347, 364, 422, 480
CviKI_1 RGCY 9 cut(s) 47, 68, 199, 244, 335, 347, 364, 422, 480
CviQI GTAC 1 cut(s) 129
DdeI CTNAG 2 cut(s) 365, 801
DpnI GATC 2 cut(s) 206, 647
DpnII GATC 2 cut(s) 204, 645
DraI TTTAAA 2 cut(s) 304, 615
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
EciI GGCGGA 1 cut(s) 387
Eco130I CCWWGG 1 cut(s) 236
Eco24I GRGCYC 1 cut(s) 482
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 663
EcoRV GATATC 1 cut(s) 118
EcoT14I CCWWGG 1 cut(s) 236
EcoT38I GRGCYC 1 cut(s) 482
ErhI CCWWGG 1 cut(s) 236
FaeI CATG 4 cut(s) 240, 311, 572, 720
FaiI YATR 7 cut(s) 215, 238, 309, 360, 376, 570, 718
FatI CATG 4 cut(s) 236, 307, 568, 716
Fnu4HI GCNGC 3 cut(s) 66, 84, 245
FokI GGATG 3 cut(s) 74, 461, 469
FriOI GRGCYC 1 cut(s) 482
Fsp4HI GCNGC 3 cut(s) 66, 84, 245
FspBI CTAG 1 cut(s) 200
FspI TGCGCA 1 cut(s) 546
GlaI GCGC 2 cut(s) 546, 703
GluI GCNGC 3 cut(s) 66, 84, 245
HaeIII GGCC 2 cut(s) 347, 422
HhaI GCGC 2 cut(s) 547, 704
Hin1II CATG 4 cut(s) 240, 311, 572, 720
Hin6I GCGC 2 cut(s) 545, 702
HinP1I GCGC 2 cut(s) 545, 702
HindIII AAGCTT 1 cut(s) 362
HinfI GANTC 5 cut(s) 4, 473, 509, 515, 791
HphI GGTGA 5 cut(s) 160, 225, 518, 579, 733
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 1 cut(s) 514
Hpy188III TCNNGA 2 cut(s) 268, 604
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 1 cut(s) 694
HpyAV CCTTC 2 cut(s) 45, 532
HpyCH4IV ACGT 2 cut(s) 672, 689
HpyCH4V TGCA 4 cut(s) 65, 86, 112, 468
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 413
HpyF3I CTNAG 2 cut(s) 365, 801
HpySE526I ACGT 2 cut(s) 672, 689
Hsp92II CATG 4 cut(s) 240, 311, 572, 720
HspAI GCGC 2 cut(s) 545, 702
Kzo9I GATC 2 cut(s) 204, 645
LpnPI CCDG 6 cut(s) 33, 117, 117, 414, 584, 661
Lsp1109I GCAGC 3 cut(s) 70, 77, 231
LweI GCATC 2 cut(s) 52, 416
MaeI CTAG 1 cut(s) 200
MaeII ACGT 2 cut(s) 672, 689
MaeIII GTNAC 2 cut(s) 166, 506
MalI GATC 2 cut(s) 206, 647
MboI GATC 2 cut(s) 204, 645
MboII GAAGA 4 cut(s) 402, 452, 455, 547
MhlI GDGCHC 1 cut(s) 482
MlsI TGGCCA 1 cut(s) 347
MluCI AATT 3 cut(s) 349, 525, 610
MluNI TGGCCA 1 cut(s) 347
MlyI GAGTC 2 cut(s) 503, 509
MmeI TCCRAC 2 cut(s) 690, 786
Mox20I TGGCCA 1 cut(s) 347
MscI TGGCCA 1 cut(s) 347
MseI TTAA 4 cut(s) 254, 303, 614, 627
MslI CAYNNNNRTG 1 cut(s) 235
Msp20I TGGCCA 1 cut(s) 347
MvnI CGCG 1 cut(s) 702
MwoI GCNNNNNNNGC 2 cut(s) 109, 413
NcoI CCATGG 1 cut(s) 236
NdeII GATC 2 cut(s) 204, 645
NlaIII CATG 4 cut(s) 240, 311, 572, 720
NlaIV GGNNCC 1 cut(s) 714
NmeAIII GCCGAG 1 cut(s) 667
NmuCI GTSAC 2 cut(s) 166, 506
NsbI TGCGCA 1 cut(s) 546
NspI RCATGY 1 cut(s) 311
PciI ACATGT 1 cut(s) 307
PcsI WCGNNNNNNNCGW 2 cut(s) 645, 654
PfeI GAWTC 3 cut(s) 4, 473, 791
PkrI GCNGC 3 cut(s) 67, 85, 246
PleI GAGTC 2 cut(s) 503, 509
PpsI GAGTC 2 cut(s) 503, 509
PscI ACATGT 1 cut(s) 307
PspN4I GGNNCC 1 cut(s) 714
PspPI GGNCC 1 cut(s) 663
RsaI GTAC 1 cut(s) 130
RsaNI GTAC 1 cut(s) 129
RseI CAYNNNNRTG 1 cut(s) 235
SaqAI TTAA 4 cut(s) 254, 303, 614, 627
SatI GCNGC 3 cut(s) 66, 84, 245
Sau3AI GATC 2 cut(s) 204, 645
Sau96I GGNCC 1 cut(s) 663
SchI GAGTC 2 cut(s) 503, 509
SduI GDGCHC 1 cut(s) 482
SetI ASST 9 cut(s) 201, 358, 366, 665, 675, 680, 692, 732, 766
SfaNI GCATC 2 cut(s) 52, 416
SinI GGWCC 1 cut(s) 663
SmiMI CAYNNNNRTG 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 3 cut(s) 349, 525, 610
SsiI CCGC 1 cut(s) 398
SspI AATATT 1 cut(s) 394
SspMI CTAG 1 cut(s) 200
StyI CCWWGG 1 cut(s) 236
TaiI ACGT 2 cut(s) 675, 692
TaqI TCGA 2 cut(s) 75, 207
TasI AATT 3 cut(s) 349, 525, 610
TfiI GAWTC 3 cut(s) 4, 473, 791
Tru1I TTAA 4 cut(s) 254, 303, 614, 627
Tru9I TTAA 4 cut(s) 254, 303, 614, 627
TscAI CASTG 2 cut(s) 475, 823
TseFI GTSAC 2 cut(s) 166, 506
TseI GCWGC 3 cut(s) 65, 83, 244
Tsp45I GTSAC 2 cut(s) 166, 506
TspDTI ATGAA 6 cut(s) 17, 230, 234, 548, 573, 623
TspRI CASTG 2 cut(s) 475, 823
VpaK11BI GGWCC 1 cut(s) 663
XapI RAATTY 1 cut(s) 610
XceI RCATGY 1 cut(s) 311
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XspI CTAG 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.