Rw7G042610

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000710
Physical Location & Seq
Reverse (-)
66334600 .. 66335205
606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G042610.1

Sequence Viewer

Length: 606 bp
ATGTCAATTATTATGTCAGTTGAAGCAATAATGAAAAACTTTGCTGATTTTAACACTAGTACTAGTGGAGCAGAAACTTCAAACCTTTCAACCACAATTAAGTGGTCTGCTCCTCCTAGTTGTTTTGTTAAAATCAATTTTGATGGCTCAGTTAGAAGAGATTCAGCTGCTAGTGATTTTGTTATCAGAGACCATAATGGAAGACCAATTATTATTGCAACCAAGAGTGTTGGCAATTCTTCTACCCCGGTTGCAAAAGCTACTGCACTTAGGGATAGTCTAATTACGGCCAGAGACAAAGGTTTTACAAGAGTTGAAGTTGAAGGAGACTCAAAGCTGGTTATTGATGCAGTTACTGGAATGGTGATTCCACCGTGGAGGCTTCTCAAATTGATTGAAGACATTAGAACCATTGCTGCTTCCTTTTCTCAAATTACCTTTAAGCATATCTATAGGGAAGCAAACTTTGTAGCTGATGCTATTGCAAATTTAGGACATGATGCTGCTTCTCCTATGTCTTGGAGTGATAGGGTCCCAAGTGAGGCCTCTAGAGCCTTAATGTTTGATGTTGTAAACTTTGGTTGCCCAAGAGGCTTCCTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

201

Amino Acids

21.86

Weight (kDa)

7.81

Isoelectric Point (pI)

33.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 46 - 164 6.2e-23 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 487
AfaI GTAC 1 cut(s) 61
AfiI CCNNNNNNNGG 1 cut(s) 541
AgsI TTSAA 6 cut(s) 23, 81, 90, 317, 323, 398
AhlI ACTAGT 2 cut(s) 56, 62
AluBI AGCT 4 cut(s) 167, 260, 337, 473
AluI AGCT 4 cut(s) 167, 260, 337, 473
Alw26I GTCTC 3 cut(s) 183, 288, 321
AlwNI CAGNNNCTG 1 cut(s) 356
AoxI GGCC 2 cut(s) 288, 543
ApeKI GCWGC 3 cut(s) 167, 416, 503
ApoI RAATTY 1 cut(s) 487
Asp700I GAANNNNTTC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 532
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 1 cut(s) 376
AvaII GGWCC 1 cut(s) 532
BbsI GAAGAC 2 cut(s) 208, 405
BbvI GCAGC 3 cut(s) 154, 403, 490
BccI CCATC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 303
BcnI CCSGG 1 cut(s) 248
BcoDI GTCTC 3 cut(s) 183, 288, 321
BcuI ACTAGT 2 cut(s) 56, 62
BfaI CTAG 5 cut(s) 57, 63, 117, 171, 549
BfmI CTRYAG 1 cut(s) 451
BglI GCCNNNNNGGC 1 cut(s) 591
BisI GCNGC 3 cut(s) 168, 417, 504
BlsI GCNGC 3 cut(s) 169, 418, 505
BmcAI AGTACT 1 cut(s) 61
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 532
BmgT120I GGNCC 1 cut(s) 532
BmiI GGNNCC 2 cut(s) 533, 534
BmrFI CCNGG 1 cut(s) 248
BmsI GCATC 3 cut(s) 337, 466, 490
BpiI GAAGAC 2 cut(s) 208, 405
BpuMI CCSGG 1 cut(s) 248
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 2 cut(s) 246, 374
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse1I ACTGG 1 cut(s) 361
Bse3DI GCAATG 1 cut(s) 411
BseDI CCNNGG 2 cut(s) 246, 374
BseLI CCNNNNNNNGG 1 cut(s) 541
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 1 cut(s) 162
BseNI ACTGG 1 cut(s) 361
BseRI GAGGAG 1 cut(s) 102
BseXI GCAGC 3 cut(s) 154, 403, 490
BsgI GTGCAG 1 cut(s) 249
BshFI GGCC 2 cut(s) 290, 545
BsiSI CCGG 1 cut(s) 248
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 3 cut(s) 183, 288, 321
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 2 cut(s) 290, 545
Bso31I GGTCTC 1 cut(s) 183
BspANI GGCC 2 cut(s) 290, 545
BspCNI CTCAG 1 cut(s) 161
BspLI GGNNCC 2 cut(s) 533, 534
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 1 cut(s) 411
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 2 cut(s) 246, 374
Bst4CI ACNGT 1 cut(s) 375
Bst6I CTCTTC 1 cut(s) 151
BstDEI CTNAG 2 cut(s) 148, 269
BstDSI CCRYGG 1 cut(s) 374
BstMAI GTCTC 3 cut(s) 183, 288, 321
BstMWI GCNNNNNNNGC 2 cut(s) 551, 591
BstSCI CCNGG 1 cut(s) 246
BstSFI CTRYAG 1 cut(s) 451
BstV1I GCAGC 3 cut(s) 154, 403, 490
BstV2I GAAGAC 2 cut(s) 208, 405
BsuRI GGCC 2 cut(s) 290, 545
BtgI CCRYGG 1 cut(s) 374
CaiI CAGNNNCTG 1 cut(s) 356
Cfr13I GGNCC 1 cut(s) 532
Csp6I GTAC 1 cut(s) 60
CviAII CATG 1 cut(s) 497
CviQI GTAC 1 cut(s) 60
DdeI CTNAG 2 cut(s) 148, 269
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 151
Eco147I AGGCCT 1 cut(s) 545
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 1 cut(s) 532
EcoO109I RGGNCCY 1 cut(s) 532
FaeI CATG 1 cut(s) 500
FaiI YATR 6 cut(s) 14, 195, 447, 453, 498, 515
FaqI GGGAC 1 cut(s) 518
FatI CATG 1 cut(s) 496
Fnu4HI GCNGC 3 cut(s) 168, 417, 504
Fsp4HI GCNGC 3 cut(s) 168, 417, 504
FspBI CTAG 5 cut(s) 57, 63, 117, 171, 549
GluI GCNGC 3 cut(s) 168, 417, 504
HaeIII GGCC 2 cut(s) 290, 545
HapII CCGG 1 cut(s) 248
Hin1II CATG 1 cut(s) 500
HinfI GANTC 3 cut(s) 161, 329, 367
HpaII CCGG 1 cut(s) 248
HphI GGTGA 1 cut(s) 376
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 1 cut(s) 188
Hpy188III TCNNGA 1 cut(s) 549
Hpy8I GTNNAC 1 cut(s) 574
HpyAV CCTTC 1 cut(s) 317
HpyCH4III ACNGT 1 cut(s) 375
HpyCH4V TGCA 5 cut(s) 218, 254, 266, 350, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 551, 591
HpyF3I CTNAG 2 cut(s) 148, 269
Hsp92II CATG 1 cut(s) 500
KflI GGGWCCC 1 cut(s) 532
LmnI GCTCC 2 cut(s) 68, 115
LpnPI CCDG 4 cut(s) 261, 304, 323, 342
Lsp1109I GCAGC 3 cut(s) 154, 403, 490
LweI GCATC 3 cut(s) 337, 466, 490
MaeI CTAG 5 cut(s) 57, 63, 117, 171, 549
MaeIII GTNAC 1 cut(s) 352
MboII GAAGA 4 cut(s) 168, 213, 231, 410
MluCI AATT 9 cut(s) 6, 96, 136, 207, 235, 282, 389, 432, 487
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 5 cut(s) 123, 372, 535, 556, 584
MroXI GAANNNNTTC 1 cut(s) 160
MseI TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
MspA1I CMGCKG 1 cut(s) 167
MspI CCGG 1 cut(s) 248
MspR9I CCNGG 1 cut(s) 248
MwoI GCNNNNNNNGC 2 cut(s) 551, 591
NciI CCSGG 1 cut(s) 248
NlaIII CATG 1 cut(s) 500
NlaIV GGNNCC 2 cut(s) 533, 534
PceI AGGCCT 1 cut(s) 545
PdmI GAANNNNTTC 1 cut(s) 160
PfeI GAWTC 2 cut(s) 161, 367
PkrI GCNGC 3 cut(s) 169, 418, 505
PleI GAGTC 1 cut(s) 323
PpsI GAGTC 1 cut(s) 323
PpuMI RGGWCCY 1 cut(s) 532
Psp5II RGGWCCY 1 cut(s) 532
PspN4I GGNNCC 2 cut(s) 533, 534
PspPI GGNCC 1 cut(s) 532
PspPPI RGGWCCY 1 cut(s) 532
PstNI CAGNNNCTG 1 cut(s) 356
PvuII CAGCTG 1 cut(s) 167
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
SaqAI TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
SatI GCNGC 3 cut(s) 168, 417, 504
Sau96I GGNCC 1 cut(s) 532
ScaI AGTACT 1 cut(s) 61
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 1 cut(s) 248
SetI ASST 7 cut(s) 87, 169, 262, 304, 339, 440, 475
SfaNI GCATC 3 cut(s) 337, 466, 490
SfcI CTRYAG 1 cut(s) 451
SinI GGWCC 1 cut(s) 532
SpeI ACTAGT 2 cut(s) 56, 62
Sse9I AATT 9 cut(s) 6, 96, 136, 207, 235, 282, 389, 432, 487
SseBI AGGCCT 1 cut(s) 545
SspMI CTAG 5 cut(s) 57, 63, 117, 171, 549
StuI AGGCCT 1 cut(s) 545
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 375
TasI AATT 9 cut(s) 6, 96, 136, 207, 235, 282, 389, 432, 487
TatI WGTACW 1 cut(s) 59
TfiI GAWTC 2 cut(s) 161, 367
Tru1I TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
Tru9I TTAA 6 cut(s) 51, 99, 129, 441, 557, 604
TseI GCWGC 3 cut(s) 167, 416, 503
TspDTI ATGAA 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 532
XapI RAATTY 1 cut(s) 487
XbaI TCTAGA 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 160
XspI CTAG 5 cut(s) 57, 63, 117, 171, 549
ZrmI AGTACT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.