Rorug03G0181700

Reverse transcriptase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000710
Physical Location & Seq
Forward (+)
15599476 .. 15600378
903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0181700.1

Sequence Viewer

Length: 903 bp
ATGGAGCACTTGCCCTGGAATTTTCTTGTTTATTTTATCATACCAGCTCTGCTCTTCTTGTTCATCCGCCGGAGATCAAACTCCAGTAACGATCATGAGCTTCCACCCGGCCCACCGGGATGGCCAATAATCGGCAACATGCTCGACCTTGGAGAGATGCCACACAAAACCCTCAACAAGCTGCGTCACACGTTTGGTCCGGTGATATGGCTAAGAATTGGTGCCCGAAACACCATGGCAATCCTCTCCGCCAAATCAGCCACCGAGTTTTTCAAAAACCACGACCTCTCCTTCGCTGAACGCTCCATCACCGAAGGTAGTCGAGTGCTCGACTACCATAAGGGGTCACTAGCTCTGGCCCCTTATGGGTCACACTGGCGCGTGTTAAAGCGCCTGTTGACGGTTGACATGTTGACCAACAAGCGTATCAATGACACTGCACCGATTCGGAGAAAGTGCGTTGACAACATGCAGACATGGATAGAAGAGGAAGCCTCTAAACTCCAAGCGAATGGAACATCATCGCGTGGGGTGCACCTGGCTCGTTTCGTGTTCCTCATGGCGTTTAATCTTCTTGGGAATCTCCTGTTGTCTAGAGACTTGGTGGATCCGAATTCGAAAGAAGGGCTGGAGTTTTTCGAGGTGATGAAGGGACTCATGGAGACGAATGGGCATGCCAACATGGCGGACTACTTTCCATGGCTGAGGTGGCTTGATCCACAAGGTGTGAAGAAGAGGATGAAGAAGGATATGGCAAAGGCTCTGAAACTAGCCTCTGGTTTTGTGAAGATGCGCTTGAATGATAGGCAGTTGCTGGGTGGAGAAAAAACTAGGGACTTTCTTGATGTGTTGCTTGAATTTGAAGGCAATGGAAATGATGAACCAGCAAAGATCGATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3

Protein Analysis

300

Amino Acids

34.35

Weight (kDa)

9.44

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 292 4.9e-30 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 221
AccII CGCG 2 cut(s) 381, 526
AciI CCGC 3 cut(s) 67, 249, 686
AclWI GGATC 3 cut(s) 602, 615, 710
AcoI YGGCCR 1 cut(s) 122
AcsI RAATTY 3 cut(s) 19, 613, 857
AdeI CACNNNGTG 1 cut(s) 725
AfiI CCNNNNNNNGG 3 cut(s) 131, 366, 400
AflIII ACRYGT 2 cut(s) 189, 408
AgsI TTSAA 4 cut(s) 274, 799, 857, 863
AjnI CCWGG 2 cut(s) 14, 537
AluBI AGCT 4 cut(s) 47, 100, 181, 353
AluI AGCT 4 cut(s) 47, 100, 181, 353
Alw21I GWGCWC 3 cut(s) 9, 330, 537
Alw26I GTCTC 2 cut(s) 591, 656
Alw44I GTGCAC 1 cut(s) 533
AlwI GGATC 3 cut(s) 602, 615, 710
AlwNI CAGNNNCTG 1 cut(s) 814
AoxI GGCC 3 cut(s) 109, 122, 357
ApaLI GTGCAC 1 cut(s) 533
ApeKI GCWGC 1 cut(s) 181
ApoI RAATTY 3 cut(s) 19, 613, 857
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AspLEI GCGC 3 cut(s) 381, 393, 795
AspS9I GGNCC 3 cut(s) 110, 197, 358
AsuC2I CCSGG 2 cut(s) 108, 117
AsuHPI GGTGA 3 cut(s) 214, 301, 655
AsuII TTCGAA 1 cut(s) 617
AvaII GGWCC 1 cut(s) 197
BaeGI GKGCMC 2 cut(s) 226, 537
BaeI ACNNNNGTAYC 2 cut(s) 409, 442
BalI TGGCCA 1 cut(s) 124
BamHI GGATCC 1 cut(s) 607
BanI GGYRCC 1 cut(s) 221
Bbv12I GWGCWC 3 cut(s) 9, 330, 537
BbvCI CCTCAGC 1 cut(s) 704
BbvI GCAGC 1 cut(s) 168
BccI CCATC 2 cut(s) 114, 314
BcgI CGANNNNNNTGC 4 cut(s) 124, 158, 524, 558
BciT130I CCWGG 2 cut(s) 16, 539
BcnI CCSGG 2 cut(s) 108, 117
BcoDI GTCTC 2 cut(s) 591, 656
BfaI CTAG 4 cut(s) 350, 594, 770, 831
BfoI RGCGCY 1 cut(s) 394
BglI GCCNNNNNGGC 1 cut(s) 683
BisI GCNGC 1 cut(s) 182
BlsI GCNGC 1 cut(s) 183
Bme1390I CCNGG 4 cut(s) 16, 108, 117, 539
Bme18I GGWCC 1 cut(s) 197
BmgT120I GGNCC 3 cut(s) 110, 197, 358
BmiI GGNNCC 3 cut(s) 223, 360, 609
BmrFI CCNGG 4 cut(s) 16, 108, 117, 539
BmsI GCATC 2 cut(s) 147, 780
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 2 cut(s) 67, 650
Bpu10I CCTNAGC 1 cut(s) 704
Bpu14I TTCGAA 1 cut(s) 617
BpuMI CCSGG 2 cut(s) 108, 117
Bsa29I ATCGAT 1 cut(s) 894
BsaJI CCNNGG 4 cut(s) 14, 148, 234, 698
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 3 cut(s) 131, 366, 400
Bse1I ACTGG 2 cut(s) 84, 380
Bse3DI GCAATG 1 cut(s) 874
BseBI CCWGG 2 cut(s) 16, 539
BseCI ATCGAT 1 cut(s) 894
BseDI CCNNGG 4 cut(s) 14, 148, 234, 698
BseGI GGATG 3 cut(s) 63, 125, 744
BseLI CCNNNNNNNGG 3 cut(s) 131, 366, 400
BseMI GCAATG 1 cut(s) 874
BseMII CTCAG 1 cut(s) 695
BseNI ACTGG 2 cut(s) 84, 380
BseSI GKGCMC 2 cut(s) 226, 537
BseXI GCAGC 1 cut(s) 168
BseYI CCCAGC 1 cut(s) 814
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 2 cut(s) 381, 526
BshFI GGCC 3 cut(s) 111, 124, 359
BshNI GGYRCC 1 cut(s) 221
BshVI ATCGAT 1 cut(s) 894
BsiHKAI GWGCWC 3 cut(s) 9, 330, 537
BsiSI CCGG 4 cut(s) 70, 108, 116, 200
BslFI GGGAC 2 cut(s) 666, 848
BslI CCNNNNNNNGG 3 cut(s) 131, 366, 400
BsmAI GTCTC 2 cut(s) 591, 656
BsmBI CGTCTC 1 cut(s) 656
BsmFI GGGAC 2 cut(s) 666, 848
BsnI GGCC 3 cut(s) 111, 124, 359
Bsp119I TTCGAA 1 cut(s) 617
Bsp1286I GDGCHC 4 cut(s) 9, 226, 330, 537
Bsp143I GATC 5 cut(s) 74, 91, 607, 715, 891
Bsp19I CCATGG 2 cut(s) 234, 698
BspACI CCGC 3 cut(s) 67, 249, 686
BspANI GGCC 3 cut(s) 111, 124, 359
BspCNI CTCAG 1 cut(s) 696
BspDI ATCGAT 1 cut(s) 894
BspFNI CGCG 2 cut(s) 381, 526
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 3 cut(s) 223, 360, 609
BspPI GGATC 3 cut(s) 602, 615, 710
BspQI GCTCTTC 1 cut(s) 59
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 874
BsrI ACTGG 2 cut(s) 84, 380
BssECI CCNNGG 4 cut(s) 14, 148, 234, 698
BssMI GATC 5 cut(s) 74, 91, 607, 715, 891
BssT1I CCWWGG 3 cut(s) 148, 234, 698
Bst2UI CCWGG 2 cut(s) 16, 539
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 3 cut(s) 59, 480, 728
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 1 cut(s) 675
BstDEI CTNAG 2 cut(s) 212, 704
BstDSI CCRYGG 2 cut(s) 234, 698
BstF5I GGATG 3 cut(s) 63, 125, 744
BstFNI CGCG 2 cut(s) 381, 526
BstH2I RGCGCY 1 cut(s) 394
BstHHI GCGC 3 cut(s) 381, 393, 795
BstKTI GATC 5 cut(s) 77, 94, 610, 718, 894
BstMAI GTCTC 2 cut(s) 591, 656
BstMBI GATC 5 cut(s) 74, 91, 607, 715, 891
BstMWI GCNNNNNNNGC 4 cut(s) 257, 532, 683, 709
BstNI CCWGG 2 cut(s) 16, 539
BstNSI RCATGY 4 cut(s) 142, 412, 472, 677
BstSCI CCNGG 4 cut(s) 14, 106, 115, 537
BstSLI GKGCMC 2 cut(s) 226, 537
BstUI CGCG 2 cut(s) 381, 526
BstV1I GCAGC 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 607
BstXI CCANNNNNNTGG 2 cut(s) 120, 512
BstYI RGATCY 1 cut(s) 607
Bsu15I ATCGAT 1 cut(s) 894
BsuRI GGCC 3 cut(s) 111, 124, 359
BsuTUI ATCGAT 1 cut(s) 894
BtgI CCRYGG 2 cut(s) 234, 698
BtgZI GCGATG 1 cut(s) 507
BtsCI GGATG 3 cut(s) 63, 125, 744
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 2 cut(s) 373, 435
Cac8I GCNNGC 1 cut(s) 675
CaiI CAGNNNCTG 1 cut(s) 814
CciI TCATGA 1 cut(s) 94
CfoI GCGC 3 cut(s) 381, 393, 795
Cfr13I GGNCC 3 cut(s) 110, 197, 358
ClaI ATCGAT 1 cut(s) 894
CseI GACGC 1 cut(s) 173
DdeI CTNAG 2 cut(s) 212, 704
DpnI GATC 5 cut(s) 76, 93, 609, 717, 893
DpnII GATC 5 cut(s) 74, 91, 607, 715, 891
DraIII CACNNNGTG 1 cut(s) 725
EaeI YGGCCR 1 cut(s) 122
Eam1104I CTCTTC 3 cut(s) 59, 480, 728
EarI CTCTTC 3 cut(s) 59, 480, 728
EciI GGCGGA 3 cut(s) 56, 238, 701
Eco130I CCWWGG 3 cut(s) 148, 234, 698
Eco47I GGWCC 1 cut(s) 197
EcoRI GAATTC 1 cut(s) 613
EcoRII CCWGG 2 cut(s) 14, 537
EcoT14I CCWWGG 3 cut(s) 148, 234, 698
ErhI CCWWGG 3 cut(s) 148, 234, 698
Esp3I CGTCTC 1 cut(s) 656
FalI AAGNNNNNCTT 2 cut(s) 779, 811
FaqI GGGAC 2 cut(s) 666, 848
Fnu4HI GCNGC 1 cut(s) 182
FokI GGATG 3 cut(s) 50, 132, 751
Fsp4HI GCNGC 1 cut(s) 182
FspBI CTAG 4 cut(s) 350, 594, 770, 831
GlaI GCGC 3 cut(s) 380, 392, 794
GluI GCNGC 1 cut(s) 182
GsaI CCCAGC 1 cut(s) 818
GsuI CTGGAG 2 cut(s) 67, 650
HaeII RGCGCY 1 cut(s) 394
HaeIII GGCC 3 cut(s) 111, 124, 359
HapII CCGG 4 cut(s) 70, 108, 116, 200
HgaI GACGC 1 cut(s) 173
HhaI GCGC 3 cut(s) 381, 393, 795
Hin6I GCGC 3 cut(s) 379, 391, 793
HinP1I GCGC 3 cut(s) 379, 391, 793
HincII GTYRAC 4 cut(s) 399, 406, 414, 463
HindII GTYRAC 4 cut(s) 399, 406, 414, 463
HinfI GANTC 3 cut(s) 445, 580, 654
HpaII CCGG 4 cut(s) 70, 108, 116, 200
HphI GGTGA 3 cut(s) 214, 301, 655
Hpy166II GTNNAC 5 cut(s) 399, 406, 414, 463, 535
Hpy188I TCNGA 4 cut(s) 450, 612, 765, 902
Hpy188III TCNNGA 3 cut(s) 95, 594, 842
Hpy8I GTNNAC 5 cut(s) 399, 406, 414, 463, 535
HpyAV CCTTC 6 cut(s) 301, 308, 617, 643, 739, 857
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4IV ACGT 1 cut(s) 191
HpyCH4V TGCA 3 cut(s) 440, 472, 535
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 532, 683, 709
HpyF3I CTNAG 2 cut(s) 212, 704
HpySE526I ACGT 1 cut(s) 191
HspAI GCGC 3 cut(s) 379, 391, 793
Kzo9I GATC 5 cut(s) 74, 91, 607, 715, 891
LguI GCTCTTC 1 cut(s) 59
LmnI GCTCC 2 cut(s) 4, 308
Lsp1109I GCAGC 1 cut(s) 168
LweI GCATC 2 cut(s) 147, 780
MaeI CTAG 4 cut(s) 350, 594, 770, 831
MaeII ACGT 1 cut(s) 191
MaeIII GTNAC 4 cut(s) 86, 185, 345, 369
MalI GATC 5 cut(s) 76, 93, 609, 717, 893
MboI GATC 5 cut(s) 74, 91, 607, 715, 891
MboII GAAGA 7 cut(s) 46, 497, 563, 742, 745, 754, 799
MflI RGATCY 1 cut(s) 607
MhlI GDGCHC 4 cut(s) 9, 226, 330, 537
MlsI TGGCCA 1 cut(s) 124
MluCI AATT 4 cut(s) 19, 216, 613, 857
MluNI TGGCCA 1 cut(s) 124
MlyI GAGTC 1 cut(s) 648
Mox20I TGGCCA 1 cut(s) 124
MscI TGGCCA 1 cut(s) 124
MseI TTAA 2 cut(s) 386, 567
MslI CAYNNNNRTG 1 cut(s) 118
Msp20I TGGCCA 1 cut(s) 124
MspI CCGG 4 cut(s) 70, 108, 116, 200
MspR9I CCNGG 4 cut(s) 16, 108, 117, 539
MvaI CCWGG 2 cut(s) 16, 539
MvnI CGCG 2 cut(s) 381, 526
MwoI GCNNNNNNNGC 4 cut(s) 257, 532, 683, 709
NciI CCSGG 2 cut(s) 108, 117
NcoI CCATGG 2 cut(s) 234, 698
NdeII GATC 5 cut(s) 74, 91, 607, 715, 891
NlaIV GGNNCC 3 cut(s) 223, 360, 609
NmuCI GTSAC 3 cut(s) 185, 345, 369
NspI RCATGY 4 cut(s) 142, 412, 472, 677
NspV TTCGAA 1 cut(s) 617
PaeI GCATGC 1 cut(s) 677
PagI TCATGA 1 cut(s) 94
PciI ACATGT 1 cut(s) 408
PciSI GCTCTTC 1 cut(s) 59
PfeI GAWTC 2 cut(s) 445, 580
PkrI GCNGC 1 cut(s) 183
PleI GAGTC 1 cut(s) 648
PpsI GAGTC 1 cut(s) 648
PscI ACATGT 1 cut(s) 408
Psp6I CCWGG 2 cut(s) 14, 537
PspFI CCCAGC 1 cut(s) 814
PspGI CCWGG 2 cut(s) 14, 537
PspN4I GGNNCC 3 cut(s) 223, 360, 609
PspPI GGNCC 3 cut(s) 110, 197, 358
PstNI CAGNNNCTG 1 cut(s) 814
PsuI RGATCY 1 cut(s) 607
RseI CAYNNNNRTG 1 cut(s) 118
SapI GCTCTTC 1 cut(s) 59
SaqAI TTAA 2 cut(s) 386, 567
SatI GCNGC 1 cut(s) 182
Sau3AI GATC 5 cut(s) 74, 91, 607, 715, 891
Sau96I GGNCC 3 cut(s) 110, 197, 358
SchI GAGTC 1 cut(s) 648
ScrFI CCNGG 4 cut(s) 16, 108, 117, 539
SduI GDGCHC 4 cut(s) 9, 226, 330, 537
SfaNI GCATC 2 cut(s) 147, 780
SfuI TTCGAA 1 cut(s) 617
SinI GGWCC 1 cut(s) 197
SmiMI CAYNNNNRTG 1 cut(s) 118
SphI GCATGC 1 cut(s) 677
Sse9I AATT 4 cut(s) 19, 216, 613, 857
SsiI CCGC 3 cut(s) 67, 249, 686
SspMI CTAG 4 cut(s) 350, 594, 770, 831
StyD4I CCNGG 4 cut(s) 14, 106, 115, 537
StyI CCWWGG 3 cut(s) 148, 234, 698
TaaI ACNGT 1 cut(s) 403
TaiI ACGT 1 cut(s) 194
TaqI TCGA 6 cut(s) 144, 322, 330, 617, 639, 894
TasI AATT 4 cut(s) 19, 216, 613, 857
TfiI GAWTC 2 cut(s) 445, 580
Tru1I TTAA 2 cut(s) 386, 567
Tru9I TTAA 2 cut(s) 386, 567
TscAI CASTG 2 cut(s) 380, 442
TseFI GTSAC 3 cut(s) 185, 345, 369
TseI GCWGC 1 cut(s) 181
Tsp45I GTSAC 3 cut(s) 185, 345, 369
TspDTI ATGAA 4 cut(s) 52, 662, 755, 894
TspRI CASTG 2 cut(s) 380, 442
VneI GTGCAC 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 197
XapI RAATTY 3 cut(s) 19, 613, 857
XbaI TCTAGA 1 cut(s) 593
XceI RCATGY 4 cut(s) 142, 412, 472, 677
XcmI CCANNNNNNNNNTGG 1 cut(s) 705
XspI CTAG 4 cut(s) 350, 594, 770, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.